##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937388_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1170862 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.24144006723252 33.0 31.0 34.0 31.0 34.0 2 32.43280249935518 34.0 31.0 34.0 31.0 34.0 3 32.47042606216616 34.0 31.0 34.0 31.0 34.0 4 36.11419791572363 37.0 35.0 37.0 35.0 37.0 5 36.057027215846105 37.0 35.0 37.0 35.0 37.0 6 36.11958283726007 37.0 35.0 37.0 35.0 37.0 7 36.06123949705431 37.0 35.0 37.0 35.0 37.0 8 36.0496967191693 37.0 35.0 37.0 35.0 37.0 9 37.748982373669996 39.0 37.0 39.0 35.0 39.0 10-11 37.67896259337138 39.0 37.0 39.0 35.0 39.0 12-13 37.582453354878716 39.0 37.0 39.0 35.0 39.0 14-15 38.72630677227547 40.0 38.0 41.0 34.5 41.0 16-17 38.71767253527743 40.0 38.0 41.0 34.5 41.0 18-19 38.792121958010426 40.0 38.0 41.0 35.0 41.0 20-21 38.74365211271696 40.0 38.0 41.0 34.5 41.0 22-23 38.593770657857206 40.0 38.0 41.0 34.0 41.0 24-25 38.535833001668856 40.0 38.0 41.0 34.0 41.0 26-27 38.44941419227885 40.0 38.0 41.0 34.0 41.0 28-29 38.31734098467625 40.0 38.0 41.0 34.0 41.0 30-31 38.27833852324185 40.0 37.5 41.0 34.0 41.0 32-33 38.12780327656034 40.0 37.0 41.0 34.0 41.0 34-35 37.97012628302909 40.0 37.0 41.0 33.5 41.0 36-37 37.77080390344891 40.0 37.0 41.0 33.0 41.0 38-39 37.61908747572301 40.0 36.0 41.0 33.0 41.0 40-41 37.398786962084344 40.0 36.0 41.0 33.0 41.0 42-43 37.128565962513086 40.0 36.0 41.0 32.5 41.0 44-45 36.73150294398486 40.0 36.0 41.0 31.0 41.0 46-47 36.17654215441273 40.0 35.0 41.0 30.0 41.0 48-49 35.68667187080971 39.0 35.0 41.0 27.5 41.0 50-51 35.384067891860866 39.0 35.0 41.0 26.5 41.0 52-53 35.119963753200636 39.0 35.0 41.0 26.0 41.0 54-55 34.89668039444444 39.0 35.0 40.0 25.0 41.0 56-57 34.64800377841283 38.5 35.0 40.0 24.0 41.0 58-59 34.399338265312224 38.0 34.0 40.0 23.0 41.0 60-61 34.08861804380021 38.0 34.0 40.0 22.5 41.0 62-63 33.72287810177459 37.5 34.0 40.0 21.5 41.0 64-65 33.33137252724916 37.0 33.0 40.0 20.0 41.0 66-67 32.920349281127926 36.0 33.0 39.0 18.5 41.0 68-69 32.5110115453401 36.0 32.0 39.0 16.0 40.0 70-71 32.11174587611521 35.0 32.0 38.5 12.0 40.0 72-73 31.642140149735837 35.0 31.5 38.0 7.5 40.0 74-75 31.177805753368034 35.0 31.0 37.0 7.0 39.0 76-77 29.89878226469046 33.5 29.5 36.0 2.0 38.5 78-79 30.46135411346512 34.0 31.0 36.0 2.0 39.0 80-81 30.26796368829119 34.0 31.0 36.0 2.0 37.5 82-83 29.926453758000516 34.0 30.5 35.5 2.0 37.0 84-85 29.57416202763434 34.0 30.0 35.0 2.0 37.0 86-87 29.15590906528694 34.0 30.0 35.0 2.0 36.0 88-89 28.703815650349913 34.0 29.0 35.0 2.0 36.0 90-91 28.21059527083465 34.0 29.0 35.0 2.0 35.5 92-93 27.902752843631447 34.0 28.5 35.0 2.0 35.0 94-95 27.52121471189602 34.0 27.0 35.0 2.0 35.0 96-97 27.085270510102813 33.0 26.5 35.0 2.0 35.0 98-99 26.412646409226706 33.0 25.0 35.0 2.0 35.0 100-101 24.110573235786966 31.0 12.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.04037911030759034 1101 2 -0.050658456341835745 1101 3 -0.05354517813174198 1101 4 0.02714683150285424 1101 5 -0.09128604098499693 1101 6 -0.0911007559494692 1101 7 -0.23508613673662637 1101 8 -0.1440543215565384 1101 9 -0.17556208280402075 1101 10-11 -0.1271744909388346 1101 12-13 -0.05440579308476856 1101 14-15 -0.20275800969106683 1101 16-17 -0.24225993118280087 1101 18-19 -0.1434200699323256 1101 20-21 -0.08401933212061152 1101 22-23 -0.15286719581895625 1101 24-25 -0.10386521273826332 1101 26-27 -0.19139050033774652 1101 28-29 -0.2782662658628041 1101 30-31 -0.26440211155364324 1101 32-33 -0.14490354627636037 1101 34-35 -0.18360776045856397 1101 36-37 -0.21931843442499854 1101 38-39 -0.32805975365111095 1101 40-41 -0.4121214230087702 1101 42-43 -0.35543872933245524 1101 44-45 -0.14584962386515343 1101 46-47 -0.3016349601833568 1101 48-49 -0.14609210846414555 1101 50-51 -0.14518463920928326 1101 52-53 -0.18499116488270317 1101 54-55 -0.36327133421348634 1101 56-57 -0.18338199816996337 1101 58-59 -0.24562242240585164 1101 60-61 -0.23179350832442935 1101 62-63 -0.19683632166482568 1101 64-65 -0.21005501007136473 1101 66-67 -0.06763882770471241 1101 68-69 -0.14425037275063346 1101 70-71 -0.08679988953038986 1101 72-73 -0.11623736648941474 1101 74-75 -0.2119235420634844 1101 76-77 -0.1504538732380567 1101 78-79 -0.36079673919463673 1101 80-81 -0.21876927875352337 1101 82-83 -0.12802497227095344 1101 84-85 -0.3767794102982158 1101 86-87 -0.361944439174799 1101 88-89 -0.07727013872402111 1101 90-91 -0.28035548674148103 1101 92-93 -0.33282862640054844 1101 94-95 -0.23408273765318555 1101 96-97 -0.18942094051937985 1101 98-99 -0.15196475886060057 1101 100-101 0.43508817991871496 1102 1 -0.13407614242158417 1102 2 -0.15631750798507227 1102 3 -0.16702555023640997 1102 4 -0.1396674193549785 1102 5 -0.1132797750735719 1102 6 -0.15563579355937662 1102 7 -0.13396866774133542 1102 8 -0.16581561054402982 1102 9 -0.3230554776093797 1102 10-11 -0.14308398653778198 1102 12-13 -0.17744192073226372 1102 14-15 -0.1908291966048381 1102 16-17 -0.27341527477323524 1102 18-19 -0.35462772570182466 1102 20-21 -0.22056903831578012 1102 22-23 -0.2372002516298437 1102 24-25 -0.4427482731210546 1102 26-27 -0.41633984848083827 1102 28-29 -0.47238487542721685 1102 30-31 -0.3182391401063782 1102 32-33 -0.3124432857260899 1102 34-35 -0.34469149363276586 1102 36-37 -0.20897919932819775 1102 38-39 -0.412278030669853 1102 40-41 -0.22288175430319512 1102 42-43 -0.13025043917551926 1102 44-45 -0.09616171630419501 1102 46-47 0.1518502725745563 1102 48-49 0.3727133258424402 1102 50-51 0.1889910469368914 1102 52-53 0.1748713893183904 1102 54-55 0.15289057847763132 1102 56-57 0.24410186783000398 1102 58-59 0.3352636602445003 1102 60-61 0.21318606249432293 1102 62-63 0.3691020491019543 1102 64-65 0.17344523777382648 1102 66-67 0.1964038882935739 1102 68-69 -0.008704319264197125 1102 70-71 0.18734696957322683 1102 72-73 0.22857352522389363 1102 74-75 0.21660680814417788 1102 76-77 0.2583292309387346 1102 78-79 0.15943729404068208 1102 80-81 0.1933539604628045 1102 82-83 0.29569333121668606 1102 84-85 0.3172865020848974 1102 86-87 0.09258655591160903 1102 88-89 0.35728116523043596 1102 90-91 0.24857310015593015 1102 92-93 0.4608510806127484 1102 94-95 0.37166943640632866 1102 96-97 0.3277783125762781 1102 98-99 0.20322290566769752 1102 100-101 0.729681304741252 1103 1 -0.05800217356609494 1103 2 -0.07974493190926069 1103 3 -0.059486619606587965 1103 4 -0.009647417088579857 1103 5 -0.08207940190876428 1103 6 -0.15696506143426348 1103 7 -0.18931775867918077 1103 8 -0.20415302426943782 1103 9 -0.1666409168027556 1103 10-11 -0.24199848804914836 1103 12-13 -0.16359950907560972 1103 14-15 -0.18890117841041842 1103 16-17 -0.2515152966465948 1103 18-19 -0.3038614992255688 1103 20-21 -0.31823860298317896 1103 22-23 -0.2899406148330499 1103 24-25 -0.23570584143578088 1103 26-27 -0.25259428574250364 1103 28-29 -0.3278067018792328 1103 30-31 -0.3426511288165628 1103 32-33 -0.3096445496374187 1103 34-35 -0.27426717678000045 1103 36-37 -0.26730280384728644 1103 38-39 -0.23460677938182783 1103 40-41 -0.3132110933323915 1103 42-43 -0.15814081937670466 1103 44-45 -0.25322404484207794 1103 46-47 -0.295571437922284 1103 48-49 -0.15536548150595308 1103 50-51 -0.12701287445604947 1103 52-53 -0.16743854303231132 1103 54-55 -0.18459061174945646 1103 56-57 -0.03016399101566236 1103 58-59 -0.17098948569825012 1103 60-61 -0.13527898097568425 1103 62-63 -0.06133618237091554 1103 64-65 -0.3516862000438934 1103 66-67 -0.13397715619895223 1103 68-69 -0.28052563235105765 1103 70-71 -0.3286970090593968 1103 72-73 -0.32915949854428206 1103 74-75 -0.14988399318508883 1103 76-77 -0.0059046611755242395 1103 78-79 -0.19240072713167677 1103 80-81 -0.17184947497257141 1103 82-83 -0.2443473057988399 1103 84-85 -0.12649711503113892 1103 86-87 -0.12368538003897456 1103 88-89 -0.10722653919984282 1103 90-91 -0.05787980491631828 1103 92-93 0.015458058478486691 1103 94-95 0.04434328350889771 1103 96-97 0.1130907458691155 1103 98-99 0.27434664300815825 1103 100-101 0.8151465880276341 1104 1 -0.030333827042390737 1104 2 -0.01829687246988243 1104 3 0.012915199274424083 1104 4 0.041903111515786406 1104 5 0.03766071511813607 1104 6 0.07031550159554456 1104 7 -0.06296814124495143 1104 8 0.007137667485352495 1104 9 0.0485074308509752 1104 10-11 0.052747524140137614 1104 12-13 0.0018397915073364857 1104 14-15 0.023505125982900665 1104 16-17 -0.04708547848280631 1104 18-19 -0.024968429111481782 1104 20-21 0.07189892005695953 1104 22-23 -0.15689357403160642 1104 24-25 -0.022296638755769038 1104 26-27 -0.1169813358724241 1104 28-29 -0.12090033896668473 1104 30-31 -0.015028559886395954 1104 32-33 -0.07140878580597843 1104 34-35 -0.1492591772320111 1104 36-37 -0.14259114791490646 1104 38-39 -0.12238623746505795 1104 40-41 -0.02656984841245702 1104 42-43 -0.14601494598461073 1104 44-45 -0.10329377783878613 1104 46-47 -0.04928077787742069 1104 48-49 -0.11433486298378881 1104 50-51 -0.06606842620001174 1104 52-53 -0.04674770495066127 1104 54-55 -0.06430018347528232 1104 56-57 -0.08957425568971189 1104 58-59 -0.13758798564607133 1104 60-61 -0.0449189696062362 1104 62-63 -0.01844015573919222 1104 64-65 -0.1611709830298551 1104 66-67 -0.10693608954643707 1104 68-69 -0.0673706855747156 1104 70-71 -0.13829104713321172 1104 72-73 0.08978043306606764 1104 74-75 0.10126087906141024 1104 76-77 0.059028606739286005 1104 78-79 -0.013913082757852635 1104 80-81 -0.1343219377898066 1104 82-83 -0.044585476124925805 1104 84-85 -0.07827756938240427 1104 86-87 -0.010455597851528609 1104 88-89 0.135780991041333 1104 90-91 0.16472815727161816 1104 92-93 0.310681737371155 1104 94-95 0.34999952606787943 1104 96-97 0.24895996954685629 1104 98-99 0.5627748860537451 1104 100-101 0.6823157512259073 1105 1 0.06997985540603935 1105 2 0.07580139195389535 1105 3 0.050001252257523277 1105 4 0.03225677333045951 1105 5 0.03402451617535718 1105 6 -0.007603971479113625 1105 7 -0.01367711769200497 1105 8 0.014476530993782433 1105 9 0.07932983411848937 1105 10-11 -0.0552605834894635 1105 12-13 0.00518660030456175 1105 14-15 0.08564166048885369 1105 16-17 0.05488072757120932 1105 18-19 -0.03986202235480363 1105 20-21 -0.06704040624043017 1105 22-23 -0.07826944771634459 1105 24-25 -0.09980378969213888 1105 26-27 0.05162459768430949 1105 28-29 0.08911191305919175 1105 30-31 0.06376643601684862 1105 32-33 0.08396019545247668 1105 34-35 -0.1094968535938392 1105 36-37 0.08027426155156547 1105 38-39 0.08014392645798551 1105 40-41 -0.037568144422110095 1105 42-43 0.20583788618616694 1105 44-45 0.05976109982623257 1105 46-47 -0.06345207213956172 1105 48-49 0.02837212302373615 1105 50-51 0.039246059129617095 1105 52-53 0.051748760313401476 1105 54-55 0.2339704751284586 1105 56-57 0.19065039811556517 1105 58-59 0.23079684678114631 1105 60-61 0.17731766330413024 1105 62-63 0.26915071247241684 1105 64-65 0.3075566473177602 1105 66-67 0.39042528404506527 1105 68-69 0.33116630607355546 1105 70-71 0.2544728463876851 1105 72-73 0.23951659983478635 1105 74-75 0.3790765951814379 1105 76-77 0.5709991830550543 1105 78-79 0.38779190751640513 1105 80-81 0.2691061590523738 1105 82-83 0.09539436067550255 1105 84-85 0.1686516306126542 1105 86-87 0.2437398240198938 1105 88-89 0.2771618996440388 1105 90-91 0.31365350226055 1105 92-93 0.46565692451893526 1105 94-95 0.560167942897916 1105 96-97 0.5346559793155379 1105 98-99 0.5175132673629044 1105 100-101 1.084688926876904 1106 1 -0.15021416625857142 1106 2 -0.0409772542365161 1106 3 -0.13518202851399508 1106 4 -0.11174546626001103 1106 5 -0.12126708764566274 1106 6 -0.12154313688886731 1106 7 -0.10851691841416056 1106 8 -0.015757940417159944 1106 9 0.022311688865052304 1106 10-11 -0.1255762971875285 1106 12-13 -0.10197094802946083 1106 14-15 -0.11571866219499327 1106 16-17 0.020481303465899714 1106 18-19 0.05922271643581212 1106 20-21 -0.02463958810432132 1106 22-23 -0.006414015566797104 1106 24-25 0.09866848966249364 1106 26-27 -0.06252881426323142 1106 28-29 -0.04025300307043267 1106 30-31 0.07015955270407659 1106 32-33 0.002892162178561364 1106 34-35 0.04782580740529596 1106 36-37 0.04752310747687716 1106 38-39 0.12301608697720923 1106 40-41 0.13607153289403584 1106 42-43 0.13172572383870573 1106 44-45 0.07304724178884925 1106 46-47 0.10642543597324305 1106 48-49 -0.07626302940553842 1106 50-51 0.06429862094688588 1106 52-53 0.13622759846871446 1106 54-55 0.15998400408317082 1106 56-57 -0.011444076037740558 1106 58-59 0.22147569407764678 1106 60-61 0.10077737352854399 1106 62-63 0.10316106486511956 1106 64-65 0.0907991401190742 1106 66-67 0.08077047806673221 1106 68-69 0.09805750311181782 1106 70-71 0.18602536249902357 1106 72-73 0.0864503033861368 1106 74-75 0.2562790495897751 1106 76-77 0.4204495692701471 1106 78-79 0.2889499814685941 1106 80-81 0.16377399122020364 1106 82-83 -9.6749521492967E-5 1106 84-85 -0.004522417561393155 1106 86-87 0.20001639059058007 1106 88-89 0.32004548664527377 1106 90-91 0.1594102781643869 1106 92-93 0.16786280687187727 1106 94-95 0.22171268900148533 1106 96-97 0.20740153441403564 1106 98-99 0.2392955700275614 1106 100-101 1.0428108702929642 1107 1 0.005448993314196571 1107 2 -0.006304848688927223 1107 3 0.015172666209423369 1107 4 -0.09228508047968376 1107 5 -0.0697486754027139 1107 6 -0.0963677821941431 1107 7 -0.10022742375450377 1107 8 -0.14201332061801963 1107 9 -0.11242448202606425 1107 10-11 -0.07958355049535726 1107 12-13 -0.019418740154435454 1107 14-15 -0.07323699473126055 1107 16-17 -0.006017182107193264 1107 18-19 0.0399217440143218 1107 20-21 0.06074021422310949 1107 22-23 0.14500077799291233 1107 24-25 0.10480988931178103 1107 26-27 -0.02840837747062608 1107 28-29 -0.03647889179990926 1107 30-31 0.01669629748329271 1107 32-33 -0.04504941282542774 1107 34-35 0.01137793557363409 1107 36-37 -0.0029491545986815026 1107 38-39 0.06196874120963258 1107 40-41 0.14352610008676692 1107 42-43 -0.09609987794672037 1107 44-45 -0.03704561649267646 1107 46-47 -0.13363514110311314 1107 48-49 -0.07683093949641062 1107 50-51 -0.2585811813805492 1107 52-53 -0.16377040886429484 1107 54-55 0.02806147897556599 1107 56-57 0.02445182703893778 1107 58-59 -0.0214204481190734 1107 60-61 -0.09120811985579991 1107 62-63 -0.047641979930034495 1107 64-65 0.01313494435947149 1107 66-67 0.06979686097761828 1107 68-69 0.0019491817344814422 1107 70-71 0.11290046213237304 1107 72-73 0.05032922894010028 1107 74-75 -0.005196719579682707 1107 76-77 0.20542884553349694 1107 78-79 0.12264797286027473 1107 80-81 0.16807615924188113 1107 82-83 0.15626384349620182 1107 84-85 0.07711714883427234 1107 86-87 -0.022552555687116893 1107 88-89 -0.12857558780076417 1107 90-91 -0.017727853511047442 1107 92-93 0.290589173841429 1107 94-95 0.17772057996274881 1107 96-97 0.07644346012740755 1107 98-99 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0.22836993988903487 2313 8 0.2782645229371141 2313 9 0.33667173709146425 2313 10-11 0.2446843475234246 2313 12-13 0.2929145142460641 2313 14-15 0.4077480869642187 2313 16-17 0.27599402635441095 2313 18-19 0.3589958619399667 2313 20-21 0.46284772073507696 2313 22-23 0.3612885670843937 2313 24-25 0.5146133465696536 2313 26-27 0.044620275146002086 2313 28-29 0.3030190954129708 2313 30-31 0.5681210350316448 2313 32-33 0.5955812604715973 2313 34-35 0.6981803667047473 2313 36-37 0.6214213560967181 2313 38-39 0.6673716932974045 2313 40-41 0.6432222086983401 2313 42-43 0.7624288141363138 2313 44-45 0.5493187374421282 2313 46-47 0.7783113424393946 2313 48-49 1.0338526187954855 2313 50-51 1.1012854221398598 2313 52-53 1.0953739592784189 2313 54-55 1.004215871587455 2313 56-57 0.882814760182832 2313 58-59 0.993300524333371 2313 60-61 1.1070198381519347 2313 62-63 1.254879923929785 2313 64-65 1.0911600449988228 2313 66-67 0.8530236349929368 2313 68-69 0.9427771871019104 2313 70-71 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28-29 0.3394555864797866 2314 30-31 0.10180958944462049 2314 32-33 0.039760805099241736 2314 34-35 0.13764916278294947 2314 36-37 0.04834251808135548 2314 38-39 -0.28148269478401744 2314 40-41 0.06243334981015636 2314 42-43 -0.0530453246201219 2314 44-45 -0.1424384100655871 2314 46-47 0.023691553465610582 2314 48-49 -0.08321714241169076 2314 50-51 -0.12088334138439194 2314 52-53 -0.9099615379353523 2314 54-55 -0.685908790904648 2314 56-57 -0.33917447786316046 2314 58-59 -0.39077389017420217 2314 60-61 -0.8477181982701936 2314 62-63 -0.8242836309619221 2314 64-65 -0.2105385053927833 2314 66-67 -0.25609913171011556 2314 68-69 -0.13324108141075186 2314 70-71 -0.0069953862343936635 2314 72-73 0.22107777370365156 2314 74-75 0.179140847168771 2314 76-77 0.16960357012098015 2314 78-79 -0.04285330197496151 2314 80-81 0.16430817925439456 2314 82-83 -0.04898583178161786 2314 84-85 0.17044470932880884 2314 86-87 0.2627315160885111 2314 88-89 0.23610786820539076 2314 90-91 0.05861793953268091 2314 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0.0329046275562348 2315 50-51 0.16265968254324292 2315 52-53 -0.1992224906578457 2315 54-55 0.08556332672131362 2315 56-57 0.05765376511892839 2315 58-59 0.08349044947475193 2315 60-61 0.14189937788584217 2315 62-63 0.23062442394612503 2315 64-65 0.21256830232941581 2315 66-67 0.29245843607822053 2315 68-69 0.18962266452992083 2315 70-71 0.2880133799282163 2315 72-73 0.03432364464183024 2315 74-75 0.15374636985121626 2315 76-77 0.2637601258159954 2315 78-79 0.0783513480758451 2315 80-81 0.13915277909898904 2315 82-83 0.17166993695107635 2315 84-85 0.13057859253961723 2315 86-87 0.4041904844705577 2315 88-89 0.7087858312679707 2315 90-91 0.500133688740739 2315 92-93 0.330135038997053 2315 94-95 0.836326969056941 2315 96-97 0.8542622989218565 2315 98-99 0.9183161681658056 2315 100-101 1.1514462436342185 2316 1 -0.8814682887026564 2316 2 -0.5230423738623458 2316 3 -0.12133811174238929 2316 4 -0.04163243633856695 2316 5 -0.06549716499770142 2316 6 0.0745727712480857 2316 7 0.06728208620297949 2316 8 0.15173621320759167 2316 9 0.05858320052782062 2316 10-11 0.26623952700147413 2316 12-13 -0.006444174628967403 2316 14-15 0.05326781618840215 2316 16-17 0.04945384049294432 2316 18-19 -0.06368458103975883 2316 20-21 -0.012147083457200836 2316 22-23 0.11557585071611243 2316 24-25 0.2630070997242555 2316 26-27 0.39622800650789713 2316 28-29 0.14643674928310446 2316 30-31 -0.07436434248089796 2316 32-33 -0.08139982678827806 2316 34-35 -0.3440184991246582 2316 36-37 -0.2589236455362567 2316 38-39 -0.23710263941243426 2316 40-41 -0.5401764433606857 2316 42-43 -0.3430119388104558 2316 44-45 -0.3909428343639405 2316 46-47 -0.41317940902285244 2316 48-49 -0.8009414787669371 2316 50-51 -0.7090883791130764 2316 52-53 -0.5362996846157699 2316 54-55 -0.2584455695811627 2316 56-57 -0.2775545875206191 2316 58-59 -0.5147411015090952 2316 60-61 -0.3051784338708714 2316 62-63 -0.3198729040741384 2316 64-65 -0.4982508988567602 2316 66-67 -0.32799533712784523 2316 68-69 -0.10521871563256724 2316 70-71 0.05474800446100403 2316 72-73 -0.17555650239427933 2316 74-75 0.025000192481183348 2316 76-77 -0.27301898022916404 2316 78-79 -0.3843073489865212 2316 80-81 -0.17844058574208077 2316 82-83 -0.16727609832523527 2316 84-85 -0.00541436455425881 2316 86-87 -0.4206542935852333 2316 88-89 -0.41317691546541724 2316 90-91 -0.1867813330366701 2316 92-93 0.06484143012546184 2316 94-95 -0.11731186577455688 2316 96-97 -0.17753580443588035 2316 98-99 -0.3335727516130227 2316 100-101 -0.4498526783069039 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 11.0 5 27.0 6 57.0 7 143.0 8 309.0 9 624.0 10 1138.0 11 1926.0 12 3281.0 13 4814.0 14 6038.0 15 8862.0 16 12704.0 17 19800.0 18 17590.0 19 9089.0 20 6761.0 21 6493.0 22 6555.0 23 6881.0 24 7779.0 25 8928.0 26 10649.0 27 12652.0 28 15767.0 29 19489.0 30 24513.0 31 30749.0 32 39302.0 33 51145.0 34 71121.0 35 104140.0 36 157064.0 37 250038.0 38 229232.0 39 25181.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.80101609529825 19.956255251207512 15.373227837660655 19.869500815833582 2 15.273021073363044 22.090391523510032 40.44737979369046 22.18920760943647 3 19.35283577398532 28.01406143507946 30.543309117556127 22.0897936733791 4 12.104415379438397 17.824474617845656 37.86090931296771 32.210200689748234 5 12.161587381302558 39.61082442088289 33.29327641663976 14.934311781174786 6 28.839521651569527 37.87807615244154 18.049778710044396 15.232623485944544 7 26.675133363282775 35.55286617893484 20.647864564739482 17.1241358930429 8 26.96372416219845 34.258606052634725 19.604189050460256 19.173480734706565 9 27.821212064274015 16.580604716866716 20.60259876911199 34.99558444974728 10-11 24.392295758294214 28.398405789241508 26.399663837268537 20.809634615195733 12-13 26.480036282914487 25.99703279555244 27.83833945877986 19.684591462753215 14-15 23.286775042660874 26.155943228151568 28.127354034890534 22.42992769429702 16-17 20.14157091100403 29.98910204618478 28.49379346156934 21.375533581241854 18-19 20.123080260526006 27.24403046644267 32.97839540441145 19.65449386861987 20-21 21.586745491782978 26.015106818736967 33.00384673855672 19.394300950923338 22-23 20.9684734121192 26.281480254139506 32.81176202307206 19.93828431066923 24-25 20.50403445352852 26.404393380037195 33.408606122462054 19.682966043972232 26-27 20.5929590859341 26.51648600831955 33.229093402991744 19.661461502754605 28-29 20.123339905596843 26.539474791163016 33.64533435483134 19.6918509484088 30-31 20.13882987468586 26.77789568756232 33.47593537215246 19.60733906559936 32-33 19.998684729711954 26.862730193652197 33.12098266063806 20.017602415997786 34-35 20.38139422066819 26.518112296752307 33.20194864979818 19.89854483278132 36-37 20.634412936793574 26.50688125500699 32.97843810799223 19.880267700207195 38-39 20.741457848556035 26.48610353711453 33.03191555234613 19.74052306198331 40-41 20.437020464863235 26.849868109821795 33.03753948484896 19.675571940466007 42-43 20.253051026618405 27.157393467653247 32.46779204971332 20.121763456015035 44-45 20.407982863848563 27.413700038774984 31.929875356362597 20.248441741013853 46-47 20.738011823767447 27.57622162133539 31.070997265262683 20.614769289634474 48-49 20.966561388105514 27.878007826712288 30.65754119614438 20.497889589037822 50-51 21.350253061419703 28.149132861088667 30.161624512538626 20.338989564953 52-53 21.012254219540814 28.567969581385338 29.958355468022706 20.461420731051142 54-55 20.693514692594004 28.625491304696883 30.050894127574384 20.63009987513473 56-57 21.027755619364193 28.92736291723534 29.549383274886367 20.4954981885141 58-59 21.002005360153458 28.233173508064997 29.936832863309252 20.82798826847229 60-61 20.885296473879926 28.32571216761668 30.237295257681946 20.551696100821445 62-63 21.231665217591825 28.175053934622525 30.325221930509315 20.26805891727633 64-65 21.015884023907173 28.210113574443447 30.399184532421415 20.37481786922797 66-67 20.740616742195066 28.54405557614817 30.090096014731028 20.625231666925735 68-69 20.726738078441354 28.74271263393978 29.89276276794362 20.637786519675245 70-71 20.838109017117304 28.71999432896447 29.577140602393794 20.864756051524434 72-73 20.837169538340127 28.567756063481436 29.412945334292175 21.182129063886265 74-75 20.832344033711912 28.85852474501692 29.263952540948463 21.045178680322703 76-77 20.93884676417887 28.47120326733637 29.782075086560155 20.807874881924597 78-79 20.790622635289214 28.56280244811088 29.57333998370431 21.073234932895595 80-81 20.627503765389843 29.241545648225685 29.280021590939665 20.850928995444807 82-83 20.67117475867046 29.076680979538498 29.0932073288167 21.15893693297434 84-85 20.694389403991607 28.646776375574795 29.05780044685256 21.601033773581037 86-87 20.744460064465326 28.581933652300613 29.24947602706382 21.42413025617024 88-89 21.013919659191263 28.1980711646633 29.28265670933039 21.505352466815047 90-91 20.596293112247405 28.4718601019076 29.34047907930403 21.59136770654097 92-93 20.364360799185185 28.730737283991743 29.162615010452097 21.742286906370975 94-95 20.22497100426865 28.650515603034343 29.104326555990372 22.020186836706632 96-97 20.28765986085465 28.54922270942263 28.81488168545909 22.34823574426363 98-99 20.28217708258157 28.526711764552655 28.746849005496383 22.444262147369393 100-101 20.185465320385298 28.857782018243306 28.98928105602397 21.967471605347427 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1714.0 1 1168.0 2 981.0 3 1230.0 4 916.5 5 817.0 6 1354.0 7 2669.0 8 3979.5 9 4607.5 10 4236.0 11 3526.5 12 3519.5 13 3668.0 14 3792.5 15 3962.5 16 4072.0 17 4206.0 18 4313.5 19 4464.0 20 4683.5 21 5043.0 22 5620.5 23 6310.5 24 7132.0 25 8263.5 26 9735.0 27 11602.0 28 13157.5 29 15134.0 30 17633.0 31 19089.0 32 20900.5 33 23291.0 34 26082.5 35 28414.5 36 31271.0 37 34461.5 38 35932.5 39 36204.0 40 36525.0 41 37614.5 42 37943.5 43 39090.0 44 41396.5 45 42603.0 46 42001.5 47 42725.0 48 50865.5 49 49693.5 50 40708.5 51 36790.5 52 32621.5 53 30367.0 54 28896.0 55 26680.5 56 23110.0 57 19766.0 58 17930.5 59 16486.0 60 14237.0 61 11751.0 62 9210.5 63 6605.0 64 4716.5 65 3441.5 66 2372.5 67 1743.0 68 1271.0 69 853.0 70 624.0 71 457.5 72 361.5 73 324.5 74 258.5 75 186.0 76 130.5 77 72.5 78 46.0 79 25.0 80 16.5 81 10.5 82 11.5 83 9.5 84 5.0 85 3.5 86 1.0 87 0.5 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07626859527425094 2 0.0 3 0.0 4 0.0 5 0.0023914005237167146 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.270358078065562E-5 12-13 0.005380651178362608 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.686644540518011E-4 24-25 8.113680348324568E-4 26-27 4.6973938858721183E-4 28-29 0.002775732750742615 30-31 3.8433222702590055E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 5.55146550148523E-4 40-41 1.2811074234196686E-4 42-43 0.012768370653416029 44-45 3.4162864624524494E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 4.270358078065562E-5 82-83 3.8433222702590055E-4 84-85 5.124429693678674E-4 86-87 0.0 88-89 0.0 90-91 0.008156383929105223 92-93 0.00380061868947835 94-95 0.0 96-97 0.0 98-99 1.2811074234196686E-4 100-101 0.011188338164531773 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1170862.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.844553770416205 #Duplication Level Percentage of deduplicated Percentage of total 1 77.4073335372902 41.67963332873164 2 12.887923613507407 13.878889919930328 3 4.026304043234083 6.503836336558852 4 1.790737994246274 3.8568595287968312 5 0.9555254805915203 2.5724921559356453 6 0.6000609888849187 1.9386009708925873 7 0.4154017030654142 1.565698353591971 8 0.2850927162422239 1.2280552071406747 9 0.22014861088675539 1.06684233447369 >10 1.2663921345250864 12.65874688452536 >50 0.09801279305715453 3.6207306034390414 >100 0.0419781303010476 3.985992445108337 >500 0.0028621429695354504 1.0734674158838644 >1k 0.001749087370271664 1.7540378807794486 >5k 1.5900794275196944E-4 0.6357927125820366 >10k+ 3.180158855039389E-4 1.980323921629748 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12867 1.0989339478093918 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10263 0.8765336991037372 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7426 0.6342335817542972 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3210 0.2741569886118091 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3052 0.26066265708512193 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2454 0.20958917447145778 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2284 0.19506995700603488 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2017 0.17226624486916475 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1547 0.1321248789353485 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1514 0.12930644260382523 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1202 0.1026594081966961 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 8.540716156131124E-5 0.0 22-23 0.0 0.0 0.0 4.6973938858721183E-4 0.0 24-25 0.0 0.0 0.0 8.113680348324568E-4 0.0 26-27 0.0 0.0 0.0 0.0011957002618583573 0.0 28-29 0.0 0.0 0.0 0.002775732750742615 0.0 30-31 0.0 0.0 0.0 0.006362833536317687 0.0 32-33 0.0 0.0 0.0 0.009565602094866859 0.0 34-35 0.0 0.0 0.0 0.01238403842639013 0.0 36-37 0.0 0.0 0.0 0.01793550392787536 0.0 38-39 0.0 0.0 0.0 0.025494037726051404 0.0 40-41 0.0 0.0 0.0 0.03471801117467302 0.0 42-43 0.0 0.0 0.0 0.04441172401188184 0.0 44-45 0.0 0.0 0.0 0.04842586060526347 0.0 46-47 0.0 0.0 0.0 0.054447065495335914 0.0 48-49 0.0 0.0 0.0 0.06597703230611293 0.0 50-51 0.0 0.0 0.0 0.07921514234811618 0.0 52-53 0.0 0.0 0.0 0.10599028749758724 0.0 54-55 0.0 0.0 0.0 0.12670152417620523 0.0 56-57 0.0 0.0 0.0 0.17679282443191427 0.0 58-59 0.0 0.0 0.0 0.2633529826743032 0.0 60-61 0.0 0.0 0.0 0.3362052914861017 0.0 62-63 0.0 0.0 0.0 0.37702991471240843 0.0 64-65 0.0 0.0 0.0 0.4559888355758407 0.0 66-67 0.0 0.0 0.0 0.5904624114541253 0.0 68-69 0.0 0.0 0.0 0.7773759845310549 0.0 70-71 0.0 0.0 0.0 0.9621543785689517 0.0 72-73 0.0 0.0 0.0 1.1723414031713388 0.0 74-75 0.0 0.0 0.0 1.4075954296919706 0.0 76-77 0.0 0.0 0.0 1.5870358761322856 0.0 78-79 0.0 0.0 0.0 1.8208806844871557 0.0 80-81 0.0 0.0 0.0 2.1332146743168705 0.0 82-83 0.0 0.0 0.0 2.4334635507856603 0.0 84-85 0.0 0.0 0.0 2.7952909907401553 0.0 86-87 0.0 0.0 0.0 3.2473938004649563 0.0 88-89 0.0 0.0 0.0 3.810440513057901 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1285 0.0 33.63774 2 CTCGACT 70 0.008392111 27.14254 4 GTACCTG 1610 0.0 26.569506 1 GTCGGGA 270 0.0 26.38858 2 GGTATCA 11245 0.0 25.233744 1 CGTACAC 135 1.3428777E-5 24.629343 3 GTATCAA 15235 0.0 24.583889 1 ACCTGGG 1770 0.0 24.15226 3 GTACATG 10505 0.0 23.97986 1 ACGTGCC 140 1.7790908E-5 23.750738 8 GAGTACT 9135 0.0 23.425741 12-13 TACATGG 10680 0.0 23.30497 2 ACATGGG 10685 0.0 22.227163 3 GTACTTT 9770 0.0 21.949932 14-15 CGGGAGT 350 0.0 21.714033 4 AGTACTT 9400 0.0 21.70413 12-13 TATCAAC 18335 0.0 20.388363 2 CATGGGG 2680 0.0 20.382225 4 TATAACG 140 4.852194E-4 20.356905 2 ATCAACG 18340 0.0 20.305107 3 >>END_MODULE