FastQCFastQC Report
Thu 26 May 2016
SRR937387_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937387_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1123186
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33650.29959419009852334No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25960.23112823699725604No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15830.14093836639701707No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15770.1404041717044194No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22000.042.3341671
GTATCAA36000.037.8823621
ATCAACG44200.030.5278033
TCAACGC45350.029.7563294
TATCAAC45750.029.493532
CAACGCA46150.029.140155
AACGCAG47300.028.5232186
GTACATG36250.025.954631
CGCAGAG54550.024.1248788
ACGCAGA54850.024.0795447
TACATGG37550.023.7874242
ACATGGG37400.022.8665373
GCAGAGT58300.021.9201979
GAGTACT32650.021.0226412-13
GTATAGG3451.8189894E-1220.6599771
GTACTTT33300.020.32563814-15
AGTACTT33550.019.32603312-13
AGAGTAC51100.019.24465810-11
GCATAAG4001.8189894E-1219.007181
GAGTACA28800.018.6441941
Produced by FastQC (version 0.11.5)