##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937387_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1123186 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.787838345563426 33.0 31.0 34.0 30.0 34.0 2 31.91022145931306 33.0 31.0 34.0 30.0 34.0 3 31.944868436750458 34.0 31.0 34.0 30.0 34.0 4 35.55015732033697 37.0 35.0 37.0 33.0 37.0 5 35.54184970254259 37.0 35.0 37.0 33.0 37.0 6 35.57539089696631 37.0 35.0 37.0 33.0 37.0 7 35.511771870375874 37.0 35.0 37.0 33.0 37.0 8 35.48799753558182 37.0 35.0 37.0 33.0 37.0 9 37.11159149063468 39.0 37.0 39.0 34.0 39.0 10-11 36.995449106381315 39.0 37.0 39.0 33.0 39.0 12-13 36.857732824305145 39.0 37.0 39.0 33.0 39.0 14-15 38.00501742365022 40.0 38.0 41.0 33.0 41.0 16-17 37.92120806349082 40.0 38.0 41.0 33.0 41.0 18-19 37.82695297127991 40.0 38.0 41.0 33.0 41.0 20-21 37.68529833883257 40.0 37.5 41.0 32.0 41.0 22-23 37.74533069322445 40.0 38.0 41.0 32.5 41.0 24-25 37.70981297843812 40.0 38.0 41.0 32.0 41.0 26-27 37.62369011009753 40.0 37.0 41.0 32.0 41.0 28-29 37.482001645319656 40.0 37.0 41.0 32.0 41.0 30-31 37.247155858424165 39.5 37.0 41.0 31.5 41.0 32-33 37.01414547545998 39.0 36.0 41.0 31.0 41.0 34-35 37.06632294205946 39.5 36.5 41.0 31.0 41.0 36-37 36.95692966258483 39.5 36.0 41.0 31.0 41.0 38-39 36.82116497178562 39.0 36.0 41.0 30.5 41.0 40-41 36.606434731202135 39.0 36.0 41.0 30.0 41.0 42-43 36.359812622308326 39.0 35.5 41.0 30.0 41.0 44-45 36.13933311134576 39.0 35.0 41.0 29.5 41.0 46-47 35.78317927751948 39.0 35.0 40.0 28.5 41.0 48-49 35.47188088170614 39.0 35.0 40.0 27.0 41.0 50-51 34.50983229847951 38.0 34.0 39.5 25.0 40.5 52-53 34.60853945829096 38.0 34.0 39.5 26.0 40.5 54-55 34.92067297847373 38.0 34.0 40.0 26.0 41.0 56-57 34.65051024496388 38.0 34.0 40.0 25.0 41.0 58-59 34.36708301207458 38.0 34.0 40.0 24.0 41.0 60-61 34.018794304772314 38.0 33.5 40.0 23.0 41.0 62-63 33.622664011125494 37.0 33.0 40.0 22.0 41.0 64-65 33.184897692813124 36.5 32.5 39.0 21.0 41.0 66-67 32.733097634763965 36.0 32.0 39.0 19.5 40.5 68-69 32.22316651026633 35.5 31.0 39.0 18.0 40.0 70-71 31.75654388498432 35.0 31.0 38.0 14.5 40.0 72-73 31.25055378183133 35.0 30.5 37.5 10.0 39.5 74-75 30.687991570407753 34.5 30.0 37.0 7.5 39.0 76-77 30.157227298061052 34.0 29.5 36.0 4.5 39.0 78-79 29.607920682772043 34.0 29.0 36.0 2.0 38.5 80-81 29.038534579312774 34.0 29.0 35.0 2.0 37.0 82-83 28.475065572398517 34.0 27.5 35.0 2.0 37.0 84-85 27.89875586056094 33.0 26.5 35.0 2.0 36.0 86-87 27.294504650164797 33.0 26.0 35.0 2.0 36.0 88-89 26.742846242741628 32.5 25.0 35.0 2.0 35.0 90-91 26.173657346156382 32.0 24.5 34.5 2.0 35.0 92-93 25.48902363455385 32.0 21.5 34.0 2.0 35.0 94-95 24.69750958434311 31.0 18.0 34.0 2.0 35.0 96-97 23.80781054963292 31.0 4.5 34.0 2.0 35.0 98-99 22.593897181766867 31.0 2.0 34.0 2.0 35.0 100-101 19.481681573666336 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23655001223860594 1101 2 0.2937260053578541 1101 3 0.25334102408810466 1101 4 0.16207065012619637 1101 5 0.14096724803228255 1101 6 0.20873421102682244 1101 7 0.19498094430813495 1101 8 0.2954161553882244 1101 9 0.37178403624331935 1101 10-11 0.3317572153046058 1101 12-13 0.4673611468335963 1101 14-15 0.5393283862634917 1101 16-17 0.5191402166946446 1101 18-19 0.472679686437381 1101 20-21 0.61672891216719 1101 22-23 0.5256876360915328 1101 24-25 0.43795280405684167 1101 26-27 0.319791742630386 1101 28-29 0.2650171168744393 1101 30-31 0.34085012011001936 1101 32-33 0.38348577998068123 1101 34-35 0.5186888318135416 1101 36-37 0.4914360618585718 1101 38-39 0.32182932300779754 1101 40-41 0.5135787987946543 1101 42-43 0.6635359018860001 1101 44-45 0.6577349198747839 1101 46-47 0.8596442859467999 1101 48-49 0.9175310294284742 1101 50-51 0.7953955278511771 1101 52-53 0.8803779128476137 1101 54-55 0.9923215637906324 1101 56-57 0.9444661158343877 1101 58-59 0.9261965501575276 1101 60-61 0.999030421782301 1101 62-63 1.0738298484181286 1101 64-65 1.0807071780203472 1101 66-67 1.0779683328465808 1101 68-69 1.1570103349839194 1101 70-71 1.144171245204717 1101 72-73 1.1751092699695356 1101 74-75 1.1659150943244647 1101 76-77 1.3032029888047916 1101 78-79 1.2713401144161587 1101 80-81 1.257862575981946 1101 82-83 0.8966195419536653 1101 84-85 1.1281105964899574 1101 86-87 1.174058523764561 1101 88-89 1.2078842255484403 1101 90-91 1.1520423736902394 1101 92-93 1.2826103446897363 1101 94-95 1.5102707185742439 1101 96-97 1.4243039412706757 1101 98-99 1.5640693880049135 1101 100-101 1.8538101875885253 1102 1 0.2835952883860493 1102 2 0.30151415618006183 1102 3 0.18795338966022612 1102 4 0.3277399904085385 1102 5 0.3232201776536314 1102 6 0.2293206532850931 1102 7 0.13041252803693482 1102 8 0.16267731305185862 1102 9 0.3434723986437618 1102 10-11 0.34396659407745034 1102 12-13 0.40089530018199326 1102 14-15 0.49101722680682514 1102 16-17 0.6243109336300634 1102 18-19 0.6047009159818302 1102 20-21 0.5161866184360449 1102 22-23 0.4239894319165529 1102 24-25 0.41713884547198177 1102 26-27 0.5210731655836369 1102 28-29 0.4124437119790514 1102 30-31 0.3882882703345558 1102 32-33 0.42681708120672823 1102 34-35 0.604376022763887 1102 36-37 0.6190045857119202 1102 38-39 0.48320613546820823 1102 40-41 0.6206772284937045 1102 42-43 0.6562637469358279 1102 44-45 0.5935880662542488 1102 46-47 0.6653604842326573 1102 48-49 0.6563681859126902 1102 50-51 0.5116615506187472 1102 52-53 0.5921585568263978 1102 54-55 0.8125896425726395 1102 56-57 0.8655235288422674 1102 58-59 0.9967429479485119 1102 60-61 0.7606769865248495 1102 62-63 0.9215481106874748 1102 64-65 0.6400000843720761 1102 66-67 0.9150068751482792 1102 68-69 0.9959423231419144 1102 70-71 1.165656197649426 1102 72-73 1.162925790603392 1102 74-75 1.2884603286344039 1102 76-77 1.2161499029900966 1102 78-79 1.1727502374981071 1102 80-81 1.2998941254438385 1102 82-83 1.3348952789910093 1102 84-85 1.3240898132122076 1102 86-87 1.3682120315403594 1102 88-89 1.5366034799642918 1102 90-91 1.3026342747465378 1102 92-93 1.537219841404493 1102 94-95 1.6175086066170898 1102 96-97 1.5165719722383955 1102 98-99 1.7446583803586115 1102 100-101 1.5834534531451006 1103 1 0.22416444892379062 1103 2 0.22328878470979419 1103 3 0.24278049814416747 1103 4 0.23437306789168133 1103 5 0.25079523045123864 1103 6 0.2541043128392175 1103 7 0.1916476853447051 1103 8 0.24231009647238722 1103 9 0.2972073869048444 1103 10-11 0.3535624702818154 1103 12-13 0.28778907124402764 1103 14-15 0.35580503783307194 1103 16-17 0.4146326569942005 1103 18-19 0.3417172655483611 1103 20-21 0.1997807554251949 1103 22-23 0.147572466862826 1103 24-25 0.3251901853438852 1103 26-27 0.17804866089042548 1103 28-29 0.21833141151311253 1103 30-31 0.24283877126312348 1103 32-33 0.31463486932124596 1103 34-35 0.36647669910788494 1103 36-37 0.4464407843472813 1103 38-39 0.4214314440373741 1103 40-41 0.5648776691158588 1103 42-43 0.5684101051984456 1103 44-45 0.4862766004858017 1103 46-47 0.47050154779578435 1103 48-49 0.7508784930429968 1103 50-51 0.7273448373266476 1103 52-53 0.5989696444020183 1103 54-55 0.8099345874476995 1103 56-57 0.6666551195008239 1103 58-59 0.8326726811377938 1103 60-61 0.7604019514967959 1103 62-63 0.8663936020157763 1103 64-65 0.837793514038438 1103 66-67 1.0566167342655604 1103 68-69 1.0613297388347966 1103 70-71 1.0437703914382581 1103 72-73 1.1116189417751663 1103 74-75 1.0840725056569482 1103 76-77 1.0564756362988987 1103 78-79 1.160415175957251 1103 80-81 1.0695572429163427 1103 82-83 1.2473457640370604 1103 84-85 1.1508216768615078 1103 86-87 1.176152036478097 1103 88-89 1.3110873371851 1103 90-91 1.2013540802563583 1103 92-93 1.2623274415862795 1103 94-95 1.385251196475231 1103 96-97 1.5620005566964785 1103 98-99 1.6425348394673982 1103 100-101 1.4118298658643305 1104 1 0.27276555108572254 1104 2 0.27464705798943356 1104 3 0.20857285366204437 1104 4 0.2823400736850914 1104 5 0.2737993282327835 1104 6 0.3444798250619101 1104 7 0.3453568841577592 1104 8 0.3621454786964975 1104 9 0.32491686832351974 1104 10-11 0.36357112507904077 1104 12-13 0.4093880891894841 1104 14-15 0.7103496895216352 1104 16-17 0.6262830738374987 1104 18-19 0.6600230448333733 1104 20-21 0.5241537241972622 1104 22-23 0.46154289924943015 1104 24-25 0.4982128291194954 1104 26-27 0.5281564042845233 1104 28-29 0.658657467751631 1104 30-31 0.5646126514383454 1104 32-33 0.6171323965220452 1104 34-35 0.8211794333172975 1104 36-37 0.7545939565954143 1104 38-39 0.8528130322809844 1104 40-41 0.930856367716963 1104 42-43 0.8963836462469104 1104 44-45 0.8987280276442036 1104 46-47 0.9719719801823885 1104 48-49 1.1062215056189544 1104 50-51 1.1283215930140855 1104 52-53 1.081819015604502 1104 54-55 1.2675251978758482 1104 56-57 1.49410584014516 1104 58-59 1.4195862744683083 1104 60-61 1.2452083666444551 1104 62-63 1.1559257130797818 1104 64-65 1.273861939925112 1104 66-67 1.3896851248265278 1104 68-69 1.2579658487683751 1104 70-71 1.0982125985881765 1104 72-73 1.2934050093179046 1104 74-75 1.3052008086101772 1104 76-77 1.407150357225845 1104 78-79 1.1484547760702917 1104 80-81 1.196725357936831 1104 82-83 1.325112649723085 1104 84-85 1.24660996992354 1104 86-87 1.3552771954437581 1104 88-89 1.372232596475758 1104 90-91 1.4224214463719456 1104 92-93 1.5055113471959984 1104 94-95 1.6547412699526909 1104 96-97 1.3598615853809513 1104 98-99 1.4039503403858689 1104 100-101 1.5775100622752412 1105 1 -0.046218119834236404 1105 2 -0.06169815779832177 1105 3 0.09969707661793237 1105 4 0.0952230188681682 1105 5 0.20349207978686934 1105 6 0.04321015729474453 1105 7 0.1256192201977342 1105 8 0.10853984531259897 1105 9 0.17045685821079815 1105 10-11 0.21745021106696072 1105 12-13 0.14869518148299932 1105 14-15 0.27027614917482623 1105 16-17 0.11171033656020057 1105 18-19 0.16917655008306554 1105 20-21 0.1768592505052311 1105 22-23 0.21265771588188898 1105 24-25 0.09070617405272685 1105 26-27 0.1479680547410851 1105 28-29 0.03504759594532203 1105 30-31 0.18534095368991643 1105 32-33 0.16010033322826445 1105 34-35 0.2047215275441161 1105 36-37 0.1026651026053429 1105 38-39 0.1573552083019294 1105 40-41 0.18691563870830663 1105 42-43 0.2440660843064535 1105 44-45 0.22626977982290697 1105 46-47 0.3046686206749385 1105 48-49 0.31533894709897226 1105 50-51 0.21951719992643604 1105 52-53 0.3039919134957074 1105 54-55 0.40945825310918593 1105 56-57 0.4870605782552744 1105 58-59 0.47311569060702396 1105 60-61 0.46032466300685115 1105 62-63 0.6170833600861201 1105 64-65 0.60443639487886 1105 66-67 0.682427012337314 1105 68-69 0.6172057955278483 1105 70-71 0.7520007815690519 1105 72-73 0.7529243573444369 1105 74-75 0.6517959426038153 1105 76-77 0.6063465441328404 1105 78-79 0.5635567584622514 1105 80-81 0.6870418639895952 1105 82-83 0.7077408805906984 1105 84-85 0.8359739463073481 1105 86-87 0.8964071811669676 1105 88-89 0.9981977670536715 1105 90-91 1.0690026381865856 1105 92-93 1.3665888731470446 1105 94-95 1.4111024355932145 1105 96-97 1.2334119705568831 1105 98-99 1.1682631954466913 1105 100-101 1.2215474570582643 1106 1 0.11910048955549257 1106 2 -0.5250386027917351 1106 3 -0.049780543876750016 1106 4 0.13918196774609015 1106 5 -0.04318836572120688 1106 6 0.031003367062979237 1106 7 0.13056287666712763 1106 8 0.19466286767455898 1106 9 0.21341339052791142 1106 10-11 -0.34848411836665605 1106 12-13 -0.10468695093891967 1106 14-15 -0.013988191698267372 1106 16-17 0.06245214820881273 1106 18-19 0.08872823477451419 1106 20-21 0.11224227422401611 1106 22-23 0.01689669807232974 1106 24-25 0.2383646482741284 1106 26-27 0.30280193665477384 1106 28-29 0.12308363147554502 1106 30-31 0.01454911478772658 1106 32-33 0.1316455051362695 1106 34-35 0.2853280421990547 1106 36-37 0.16952172545576616 1106 38-39 0.39079457321196287 1106 40-41 0.5052109691080844 1106 42-43 0.3149931968087074 1106 44-45 0.4860240415553889 1106 46-47 0.5199755917393745 1106 48-49 0.5981356308195913 1106 50-51 0.43539088140359894 1106 52-53 0.61991425369974 1106 54-55 0.8828977232905046 1106 56-57 0.507913357534143 1106 58-59 0.38054801871655997 1106 60-61 0.5533678101971589 1106 62-63 0.6662821871300793 1106 64-65 0.6843454872766301 1106 66-67 0.8270045469774132 1106 68-69 0.8944994228721832 1106 70-71 0.8569003995512645 1106 72-73 0.7997586743794365 1106 74-75 0.5292511028851905 1106 76-77 0.5272413149572515 1106 78-79 0.7952144068889133 1106 80-81 0.9400406334852569 1106 82-83 0.9244137465663194 1106 84-85 0.7166106387303905 1106 86-87 0.6956229685844733 1106 88-89 0.9806060582200047 1106 90-91 0.7987490279663803 1106 92-93 1.2045950818134443 1106 94-95 1.0452943732164677 1106 96-97 0.8737660797629694 1106 98-99 1.0513085533339677 1106 100-101 0.9011490403383782 1107 1 0.014588859356400974 1107 2 0.031218173277650862 1107 3 0.07651614940138884 1107 4 0.010057805514669838 1107 5 0.16779995479920018 1107 6 0.24944536390903238 1107 7 0.18022530521039215 1107 8 0.15903614285689827 1107 9 0.17103215654407222 1107 10-11 0.3439439320965789 1107 12-13 0.1894006205178087 1107 14-15 0.1727987810003313 1107 16-17 0.19104654501544616 1107 18-19 0.23587674157523253 1107 20-21 0.19418035326994953 1107 22-23 0.34281639549003273 1107 24-25 0.32667069252801895 1107 26-27 0.2535245120539429 1107 28-29 0.2061120416864668 1107 30-31 0.3794466113380963 1107 32-33 0.30449533511853843 1107 34-35 0.3651408994827108 1107 36-37 0.3290966195026783 1107 38-39 0.5992572678699517 1107 40-41 0.6137158601981625 1107 42-43 0.4429022865977785 1107 44-45 0.5450312857895696 1107 46-47 0.7679658711598307 1107 48-49 0.6237183727868683 1107 50-51 0.5380529526632145 1107 52-53 0.6003144729979937 1107 54-55 0.8504434309585562 1107 56-57 0.7143478514233621 1107 58-59 0.6850507168241933 1107 60-61 0.8238346197772941 1107 62-63 0.7913345476662528 1107 64-65 0.52587478453664 1107 66-67 0.6414434268315432 1107 68-69 0.8654454853598601 1107 70-71 0.9191986574854241 1107 72-73 0.9291153789920941 1107 74-75 0.9283015291615548 1107 76-77 1.1478615101080507 1107 78-79 1.1400470379750551 1107 80-81 1.0827646749794404 1107 82-83 1.0569813840589255 1107 84-85 0.9827315238082512 1107 86-87 1.2076150713836071 1107 88-89 1.2303427155233777 1107 90-91 1.0125216969985082 1107 92-93 1.1547555514566525 1107 94-95 1.2323924291005603 1107 96-97 1.1616982081879677 1107 98-99 1.5227982413883723 1107 100-101 1.3297970296937862 1108 1 0.04239556136321454 1108 2 0.005757883614645465 1108 3 0.07180084269938192 1108 4 0.003790056555942556 1108 5 0.020698951391395326 1108 6 0.06203292543874994 1108 7 0.04319861077646436 1108 8 -0.025256028669041086 1108 9 0.017037457566416947 1108 10-11 -0.020856332954537038 1108 12-13 0.019727674663961636 1108 14-15 -0.010046850389294093 1108 16-17 0.10512856887004318 1108 18-19 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88-89 0.91176126508606 2315 90-91 1.083360464836474 2315 92-93 1.5099695590521733 2315 94-95 1.7502891319546343 2315 96-97 2.2687415659446053 2315 98-99 2.786518367596752 2315 100-101 3.180893201245116 2316 1 -0.2901579557253129 2316 2 -0.33675851891125674 2316 3 -0.40623824192533675 2316 4 -0.4703159398205514 2316 5 -0.7230804016542791 2316 6 -0.4264932218585713 2316 7 -0.6160123089962752 2316 8 -0.28056903987376813 2316 9 -0.3652566093805163 2316 10-11 -0.5560448080913005 2316 12-13 -0.5262046748109768 2316 14-15 -0.8027382718242748 2316 16-17 -0.7314477446512697 2316 18-19 -0.5525453566103309 2316 20-21 -0.6078741603061673 2316 22-23 -0.7748546732269901 2316 24-25 -0.5554058983225758 2316 26-27 -0.8808915058330697 2316 28-29 -0.48745820345572355 2316 30-31 -0.5656055055065821 2316 32-33 -0.32306727747958774 2316 34-35 -0.43564688522968 2316 36-37 -0.7025605433463795 2316 38-39 -0.370948464982952 2316 40-41 -0.20347606329945478 2316 42-43 -0.015785261658557204 2316 44-45 -0.17857651886877335 2316 46-47 -0.3340771815360952 2316 48-49 -0.2837380356547712 2316 50-51 -0.45756879003399575 2316 52-53 -0.14282005762869687 2316 54-55 -0.26887237460058344 2316 56-57 -0.12451317836688958 2316 58-59 -0.2732286632427261 2316 60-61 -0.34213300022013726 2316 62-63 -0.21003874727648508 2316 64-65 0.1163574283405211 2316 66-67 0.30113872827280375 2316 68-69 -0.007425203740641706 2316 70-71 -0.11996035518991022 2316 72-73 -0.27290073058196285 2316 74-75 -0.08985391254478614 2316 76-77 -0.030377294421612078 2316 78-79 -0.011599508479712028 2316 80-81 0.10415128062864554 2316 82-83 -0.058855943826802815 2316 84-85 0.6581312471385843 2316 86-87 0.5744507022547936 2316 88-89 0.32300393190479326 2316 90-91 0.3867777451261638 2316 92-93 0.0554691420498834 2316 94-95 0.368860346678737 2316 96-97 0.391208366147044 2316 98-99 0.9389558707153611 2316 100-101 1.6291029427036925 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4533.0 3 875.0 4 920.0 5 1006.0 6 1119.0 7 1302.0 8 1594.0 9 1960.0 10 2636.0 11 3013.0 12 4195.0 13 5065.0 14 5662.0 15 7061.0 16 8489.0 17 10809.0 18 9764.0 19 7690.0 20 8050.0 21 8655.0 22 9636.0 23 10733.0 24 12116.0 25 13823.0 26 16072.0 27 18913.0 28 22322.0 29 26880.0 30 32814.0 31 40030.0 32 49798.0 33 64518.0 34 86306.0 35 117365.0 36 162208.0 37 200937.0 38 131905.0 39 12412.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.38397272256637 18.51661102879483 14.738382990877769 23.361033257761036 2 15.176930188066404 19.496229034825657 40.79192053311061 24.53492024399733 3 19.08663966455272 23.760075598780354 29.99527953791227 27.158005198754655 4 12.66182312692073 15.981271884269024 37.544214700517905 33.81269028829234 5 13.919867933788426 36.42252264310891 34.97809307098559 14.679516352117075 6 32.51661974243414 35.40165715692677 17.660282830012953 14.421440270626142 7 28.63023084032444 31.865884947383822 21.25334984316846 18.25053436912328 8 26.34885884833027 34.46427026154199 20.193071996531447 18.993798893596296 9 25.881434491865296 16.518783206862864 20.63810709171451 36.961675209557335 10-11 24.67306614699888 26.121604402899575 28.0113338258756 21.19399562422594 12-13 25.4580365001635 23.34394638779182 28.478808911039945 22.719208201004736 14-15 22.63182639757482 24.798511204329355 26.473540154125324 26.096122243970505 16-17 22.133249832507694 28.026213549793166 27.130007038691396 22.710529579007748 18-19 22.157987810725047 26.727204204920756 29.245652023629265 21.869155960724935 20-21 22.658689910054086 25.845467512691712 29.500797051222694 21.995045526031507 22-23 22.586417525738707 25.54125664402817 29.537731300492272 22.334594529740848 24-25 22.357942419172616 25.79116881077915 29.525015488189055 22.32587328185917 26-27 22.156991649019773 26.021572324912185 29.410980263427845 22.410455762640204 28-29 22.19081051424901 26.213764156455877 29.322541939685358 22.272883389609753 30-31 22.259208684988497 25.911098957424873 29.34297380379995 22.486718553786677 32-33 22.043183181709708 25.981011176062573 29.368756537584 22.60704910464372 34-35 22.426538153412995 26.031918367201285 29.152697511512844 22.388845967872875 36-37 22.314535426995032 25.90346587095033 29.222013331816832 22.5599853702378 38-39 22.160782683624337 26.100024441023095 29.246270959961322 22.492921915391243 40-41 22.42417701217913 26.06658829112174 29.12162897445183 22.387605722247297 42-43 22.070276521846953 26.05434630863383 29.038429030132974 22.836948139386244 44-45 22.27904771631833 26.115459869491165 29.01437773705292 22.591114677137583 46-47 22.455375343120767 26.264562281827825 28.570128666385358 22.709933708666046 48-49 22.472282997785317 26.37909231417732 28.402104507204974 22.74652018083239 50-51 22.54812086725292 26.481610599817373 28.447307154082687 22.522961378847025 52-53 22.50080968011613 26.614163111955342 28.154964298237083 22.730062909691448 54-55 22.4696712942374 26.50825009489918 28.1191942558232 22.90288435504023 56-57 22.370293658662334 26.763555337908386 28.25649005579885 22.609660947630434 58-59 22.570600858751902 26.43141669089748 28.26612032873037 22.731862121620246 60-61 22.42571767006087 26.42900721416288 28.468248050178037 22.677027065598217 62-63 22.27469656410755 26.33405198750844 28.759398328571546 22.63185311981247 64-65 22.31045527482123 26.314970293815172 28.54902167155601 22.82555275980759 66-67 22.22998228119867 26.487890963143734 28.297278047818907 22.984848707838683 68-69 22.139249121185824 26.325981413113574 28.631681195383518 22.903088270317088 70-71 22.27141622991932 26.199890015935644 28.51316798259522 23.015525771549807 72-73 22.21451132802709 26.364746505344456 28.29531456136955 23.125427605258906 74-75 22.231617970081384 26.54127803281584 28.14911051852486 23.07799347857792 76-77 22.362009529641753 26.380908246311314 28.223858715456384 23.03322350859055 78-79 22.300043978237465 26.248986404212634 28.186392794348663 23.264576823201242 80-81 22.298075422046786 26.2655605361165 28.17372493922972 23.26263910260699 82-83 22.357013962541178 26.167521949992462 28.259985317611463 23.215478769854897 84-85 22.22234609914782 26.05663920612531 28.243834979880656 23.47717971484622 86-87 22.278453013049727 25.957769229191214 28.40596411080181 23.357813646957247 88-89 22.25043350126304 25.632635330888125 28.4314390101186 23.685492157730238 90-91 22.121721896008697 25.732464078108098 28.67819746983578 23.467616556047428 92-93 22.03337745839637 25.664061384979874 28.714355036679702 23.58820611994405 94-95 21.890559245324756 25.872878680031896 28.584680649137734 23.65188142550561 96-97 21.935547175533706 25.924477221182773 28.55405968944363 23.585915913839898 98-99 21.842987429574574 26.100447440064794 28.30473201765497 23.751833112705654 100-101 22.372950143384042 25.288797313388905 28.62217524518002 23.716077298047033 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1417.0 1 801.0 2 167.0 3 145.0 4 128.5 5 127.0 6 199.0 7 342.0 8 571.0 9 753.5 10 780.0 11 797.0 12 844.5 13 910.0 14 973.0 15 1056.5 16 1112.5 17 1194.5 18 1311.0 19 1422.5 20 1571.5 21 1795.0 22 2151.5 23 2578.0 24 3139.5 25 4018.0 26 5265.0 27 6714.0 28 8176.5 29 9945.5 30 11873.5 31 13712.5 32 16036.5 33 18200.0 34 20652.5 35 23429.5 36 26212.0 37 28899.0 38 30724.0 39 31975.0 40 33435.5 41 35191.5 42 36856.0 43 38920.0 44 41263.5 45 43153.5 46 44553.0 47 45601.5 48 45895.0 49 45301.5 50 43624.0 51 41699.5 52 40370.5 53 39730.0 54 39051.5 55 36223.0 56 32508.0 57 29031.0 58 26109.5 59 23094.5 60 19003.5 61 15280.5 62 11987.5 63 9056.0 64 6725.0 65 4973.5 66 3583.0 67 2660.5 68 1999.0 69 1471.0 70 1081.5 71 733.5 72 498.5 73 368.0 74 251.5 75 146.0 76 93.5 77 60.5 78 48.0 79 38.5 80 23.5 81 16.0 82 12.0 83 8.5 84 7.0 85 6.0 86 5.5 87 5.0 88 4.0 89 3.5 90 2.5 91 2.0 92 2.0 93 1.5 94 1.0 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25338634918882535 2 0.2241837059934864 3 0.22551919272498053 4 0.2241837059934864 5 0.22578629007127937 6 0.1985423607487985 7 0.1992546203389287 8 0.20094623686548801 9 0.2110959360248436 10-11 0.21991014845270507 12-13 0.2133662634683837 14-15 0.20299398318711237 16-17 0.199343652787695 18-19 0.1886152427113586 20-21 0.19400170586171836 22-23 0.19235460555954223 24-25 0.193244930047205 26-27 0.18874879138450798 28-29 0.19863139319756476 30-31 0.2097159330689663 32-33 0.2015249477824688 34-35 0.2015694640068519 36-37 0.1944023518811666 38-39 0.18874879138450798 40-41 0.18589975302398712 42-43 0.19333396249597126 44-45 0.1880810480187609 46-47 0.19809719850496713 48-49 0.2010797855386374 50-51 0.20802431654240705 52-53 0.21127400092237617 54-55 0.2011688179874037 56-57 0.18745782087739696 58-59 0.18020167630294537 60-61 0.1832287795609988 62-63 0.18776943444807895 64-65 0.18153716303443954 66-67 0.18345136068291448 68-69 0.18456426629249295 70-71 0.18825911291629346 72-73 0.18848169403820916 74-75 0.18799201556999465 76-77 0.19208750821324339 78-79 0.1938236409641858 80-81 0.19239912178392538 82-83 0.1883036291406766 84-85 0.17819844620570413 86-87 0.17922231936651634 88-89 0.18536555833138946 90-91 0.18456426629249295 92-93 0.18963911587217078 94-95 0.18411910404866158 96-97 0.18995072944285274 98-99 0.1907075052573661 100-101 0.1843862013949604 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1123186.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.337612766125474 #Duplication Level Percentage of deduplicated Percentage of total 1 80.17972008320535 49.98212342243189 2 11.403190888175597 14.216953957706016 3 3.5563979696247876 6.650920784181145 4 1.5522375024664015 3.87051121399233 5 0.8366359812209665 2.6076944911780013 6 0.516723115121857 1.9326771274663435 7 0.3466708206902915 1.5127441964254416 8 0.25998418972360837 1.2965434995444158 9 0.19856545278459686 1.1140286673974915 >10 1.074566100521278 11.843637596300425 >50 0.056710857090663654 2.420024530380592 >100 0.017881770241614237 1.6763396732277895 >500 1.430538265971385E-4 0.06242343832987416 >1k 5.72215306388554E-4 0.8133774014382602 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3365 0.29959419009852334 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2596 0.23112823699725604 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1583 0.14093836639701707 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1577 0.1404041717044194 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 8.903244876627735E-5 0.0 10-11 0.0 0.0 0.0 8.903244876627735E-5 0.0 12-13 0.0 0.0 0.0 1.780648975325547E-4 0.0 14-15 0.0 0.0 0.0 1.780648975325547E-4 0.0 16-17 0.0 0.0 0.0 2.2258112191569338E-4 0.0 18-19 0.0 0.0 0.0 3.561297950651094E-4 0.0 20-21 0.0 0.0 0.0 4.451622438313868E-4 0.0 22-23 0.0 0.0 0.0 8.012920388964963E-4 0.0 24-25 0.0 0.0 0.0 0.001380002955877299 0.0 26-27 0.0 0.0 0.0 0.001825165199708686 0.0 28-29 0.0 0.0 0.0 0.0027154896873714594 0.0 30-31 0.0 0.0 0.0 0.005119365804060949 0.0 32-33 0.0 0.0 0.0 0.008814212427861458 0.0 34-35 0.0 0.0 0.0 0.01344389976370788 0.0 36-37 0.0 0.0 0.0 0.020076817196795542 0.0 38-39 0.0 0.0 0.0 0.029425224317254667 0.0 40-41 0.0 4.4516224383138675E-5 0.0 0.041978799593299775 0.0 42-43 0.0 8.903244876627735E-5 0.0 0.0619220681169459 0.0 44-45 0.0 8.903244876627735E-5 0.0 0.0786156522606229 0.0 46-47 0.0 8.903244876627735E-5 0.0 0.11173572320167809 0.0 48-49 0.0 8.903244876627735E-5 0.0 0.15585130156536853 0.0 50-51 0.0 8.903244876627735E-5 4.4516224383138675E-5 0.17802038130817158 0.0 52-53 0.0 8.903244876627735E-5 8.903244876627735E-5 0.21505787999494297 0.0 54-55 0.0 8.903244876627735E-5 8.903244876627735E-5 0.27978447024802655 0.0 56-57 0.0 8.903244876627735E-5 8.903244876627735E-5 0.3774085503202497 0.0 58-59 0.0 8.903244876627735E-5 8.903244876627735E-5 0.505259146748624 0.0 60-61 0.0 8.903244876627735E-5 8.903244876627735E-5 0.6446839615166143 0.0 62-63 0.0 8.903244876627735E-5 8.903244876627735E-5 0.7918100831028877 0.0 64-65 0.0 8.903244876627735E-5 8.903244876627735E-5 0.955318175262156 0.0 66-67 0.0 8.903244876627735E-5 8.903244876627735E-5 1.1664141112869997 0.0 68-69 0.0 8.903244876627735E-5 8.903244876627735E-5 1.4211804634317022 0.0 70-71 0.0 8.903244876627735E-5 8.903244876627735E-5 1.579302092440611 0.0 72-73 0.0 8.903244876627735E-5 8.903244876627735E-5 1.8002361140541283 0.0 74-75 0.0 8.903244876627735E-5 8.903244876627735E-5 2.055358595993896 0.0 76-77 0.0 8.903244876627735E-5 8.903244876627735E-5 2.332160479208252 0.0 78-79 0.0 8.903244876627735E-5 8.903244876627735E-5 2.64444179325597 0.0 80-81 0.0 8.903244876627735E-5 8.903244876627735E-5 3.095124048910866 0.0 82-83 0.0 8.903244876627735E-5 8.903244876627735E-5 3.5964657679137737 0.0 84-85 0.0 8.903244876627735E-5 8.903244876627735E-5 4.233893584855936 0.0 86-87 0.0 8.903244876627735E-5 8.903244876627735E-5 4.967476446465679 0.0 88-89 0.0 8.903244876627735E-5 8.903244876627735E-5 5.883976474065738 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2200 0.0 42.334167 1 GTATCAA 3600 0.0 37.882362 1 ATCAACG 4420 0.0 30.527803 3 TCAACGC 4535 0.0 29.756329 4 TATCAAC 4575 0.0 29.49353 2 CAACGCA 4615 0.0 29.14015 5 AACGCAG 4730 0.0 28.523218 6 GTACATG 3625 0.0 25.95463 1 CGCAGAG 5455 0.0 24.124878 8 ACGCAGA 5485 0.0 24.079544 7 TACATGG 3755 0.0 23.787424 2 ACATGGG 3740 0.0 22.866537 3 GCAGAGT 5830 0.0 21.920197 9 GAGTACT 3265 0.0 21.02264 12-13 GTATAGG 345 1.8189894E-12 20.659977 1 GTACTTT 3330 0.0 20.325638 14-15 AGTACTT 3355 0.0 19.326033 12-13 AGAGTAC 5110 0.0 19.244658 10-11 GCATAAG 400 1.8189894E-12 19.00718 1 GAGTACA 2880 0.0 18.644194 1 >>END_MODULE