FastQCFastQC Report
Thu 26 May 2016
SRR937387_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937387_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1123186
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32030.28517093339838634No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26610.23691534616706406No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16150.14378740475753793No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24900.038.7390251
GTATCAA38500.035.422011
TCAACGC50050.027.140674
ATCAACG50000.026.9778253
CAACGCA50800.026.7399715
TATCAAC51300.026.2941762
AACGCAG51900.025.9901986
GTACATG38700.023.5744881
TACATGG39350.022.6919442
ACGCAGA60650.022.0878937
CGCAGAG60950.021.6674128
ACATGGG39150.021.5946833
AAGACCG2651.8562787E-819.7153915
GAGTACT34600.019.63341712-13
GCAGAGT66200.019.5902799
GTACTTT35400.019.18630614-15
AGAGTAC56500.019.17001210-11
GGACCAT5700.019.1651216
AGTACTT36800.019.04057912-13
AGCGACC1559.618037E-418.388898
Produced by FastQC (version 0.11.5)