##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937387_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1123186 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.28474713894226 33.0 31.0 34.0 31.0 34.0 2 32.49995014182869 34.0 31.0 34.0 31.0 34.0 3 32.57131766243525 34.0 31.0 34.0 31.0 34.0 4 36.17171243231308 37.0 35.0 37.0 35.0 37.0 5 36.09275400512471 37.0 35.0 37.0 35.0 37.0 6 36.125609649692926 37.0 35.0 37.0 35.0 37.0 7 36.066297122649324 37.0 35.0 37.0 35.0 37.0 8 36.03976901421492 37.0 35.0 37.0 35.0 37.0 9 37.75893218042248 39.0 37.0 39.0 35.0 39.0 10-11 37.679062060958735 39.0 37.0 39.0 35.0 39.0 12-13 37.575497735904825 39.0 37.0 39.0 35.0 39.0 14-15 38.84687442685362 40.0 38.0 41.0 35.0 41.0 16-17 38.79800763186151 40.0 38.0 41.0 35.0 41.0 18-19 38.75537577925651 40.0 38.0 41.0 35.0 41.0 20-21 38.630791338211125 40.0 38.0 41.0 34.0 41.0 22-23 38.570965539100385 40.0 38.0 41.0 34.0 41.0 24-25 38.60425477169409 40.0 38.0 41.0 34.0 41.0 26-27 38.46032446985628 40.0 38.0 41.0 34.0 41.0 28-29 38.315575069489825 40.0 38.0 41.0 34.0 41.0 30-31 38.41576907119568 40.0 38.0 41.0 34.0 41.0 32-33 38.348028287389624 40.0 38.0 41.0 34.0 41.0 34-35 38.23438994075781 40.0 38.0 41.0 34.0 41.0 36-37 38.10842460643206 40.0 38.0 41.0 33.5 41.0 38-39 38.00590641265116 40.0 38.0 41.0 33.0 41.0 40-41 37.8376128263707 40.0 37.0 41.0 33.0 41.0 42-43 37.65865582370151 40.0 37.0 41.0 33.0 41.0 44-45 37.39179797468985 40.0 37.0 41.0 32.5 41.0 46-47 37.102126005844084 40.0 37.0 41.0 31.5 41.0 48-49 36.832347447350664 39.5 36.0 41.0 31.0 41.0 50-51 36.604429275293676 39.0 36.0 41.0 31.0 41.0 52-53 36.36041181068852 39.0 35.5 40.5 30.0 41.0 54-55 36.149858082276666 39.0 35.0 40.0 30.0 41.0 56-57 35.83843459587281 38.5 35.0 40.0 29.0 41.0 58-59 35.570580918921706 38.0 35.0 40.0 28.5 41.0 60-61 35.20897607341972 38.0 34.0 40.0 28.0 41.0 62-63 34.828877407659995 37.5 34.0 40.0 27.5 41.0 64-65 34.40406442031863 37.0 34.0 39.5 26.5 41.0 66-67 33.96469507276622 36.0 33.0 39.0 26.0 40.5 68-69 33.50051015593143 36.0 33.0 39.0 26.0 40.0 70-71 33.05471845268727 35.0 32.0 38.0 25.0 40.0 72-73 32.5680813329226 35.0 32.0 37.5 24.5 39.5 74-75 32.08605431335504 35.0 31.0 37.0 23.0 39.0 76-77 30.77258219030508 33.5 29.5 35.5 21.5 38.0 78-79 31.433304011980205 34.0 31.0 36.0 23.0 38.0 80-81 31.261943257839754 34.0 31.0 35.5 22.0 37.0 82-83 30.879700690713737 34.0 31.0 35.0 20.5 37.0 84-85 30.475503166884202 34.0 30.5 35.0 19.0 36.0 86-87 30.114474806487973 34.0 30.0 35.0 18.0 36.0 88-89 29.692427167005285 34.0 30.0 35.0 13.5 35.5 90-91 29.156477199680197 34.0 29.0 35.0 7.0 35.0 92-93 28.663449775905327 33.5 29.0 35.0 2.0 35.0 94-95 28.19550991554382 33.0 29.0 35.0 2.0 35.0 96-97 27.543332092814545 33.0 27.0 35.0 2.0 35.0 98-99 26.57433274631272 32.5 25.5 34.0 2.0 35.0 100-101 23.597511454024534 29.5 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15687640808938852 1101 2 0.1852404804458061 1101 3 0.14791520173627504 1101 4 0.12402213815092011 1101 5 0.0873638810988453 1101 6 0.19782490455794033 1101 7 0.24213203573224007 1101 8 0.15395881457008898 1101 9 0.21655180677225871 1101 10-11 0.2143466038959687 1101 12-13 0.21699093187173446 1101 14-15 0.3559364194450012 1101 16-17 0.23985771336786144 1101 18-19 0.28274982343646116 1101 20-21 0.320376867876476 1101 22-23 0.2548687465084427 1101 24-25 0.282610842826692 1101 26-27 0.1450503314737972 1101 28-29 0.12709691701704173 1101 30-31 0.3328533996763525 1101 32-33 0.30098369643950207 1101 34-35 0.27069313491939084 1101 36-37 0.27738238308459984 1101 38-39 0.25873094517121586 1101 40-41 0.3511659037573338 1101 42-43 0.2635533539636228 1101 44-45 0.28207033363425893 1101 46-47 0.3464920696799041 1101 48-49 0.31894510420382716 1101 50-51 0.3685586577455595 1101 52-53 0.3053845834940887 1101 54-55 0.2526026470825755 1101 56-57 0.38054495310128544 1101 58-59 0.4053439475207483 1101 60-61 0.2930968515461032 1101 62-63 0.3967560191773458 1101 64-65 0.5637185666071858 1101 66-67 0.5257597809003514 1101 68-69 0.40986080843457273 1101 70-71 0.3220132789661747 1101 72-73 0.3664838256980545 1101 74-75 0.5285255774643396 1101 76-77 0.5590171167489828 1101 78-79 0.5477192016989427 1101 80-81 0.5976679936545857 1101 82-83 0.4962274332216978 1101 84-85 0.4421775803718013 1101 86-87 0.5921010733145593 1101 88-89 0.9858197746498938 1101 90-91 1.0138881492622644 1101 92-93 1.069407568989007 1101 94-95 0.959990001156001 1101 96-97 1.0957334554129083 1101 98-99 1.582290767622876 1101 100-101 2.258734247148542 1102 1 0.2070957561415554 1102 2 0.23655238500848697 1102 3 0.20732445619196938 1102 4 0.10470675908321425 1102 5 0.13194751825214013 1102 6 0.19408024880090124 1102 7 0.17676910535382717 1102 8 0.14149602006394701 1102 9 0.16678669446844907 1102 10-11 0.2105222576567627 1102 12-13 0.18480434457678996 1102 14-15 0.29402886837336695 1102 16-17 0.23215522981399062 1102 18-19 0.2443688949626477 1102 20-21 0.19744194710872875 1102 22-23 0.17404626104199394 1102 24-25 0.12910426238513395 1102 26-27 0.23293777663678839 1102 28-29 0.1768488803912902 1102 30-31 0.28769200652733673 1102 32-33 0.3048180039889772 1102 34-35 0.2980094383966829 1102 36-37 0.297197421475488 1102 38-39 0.34580196134439944 1102 40-41 0.42470428070129174 1102 42-43 0.42725155016955085 1102 44-45 0.5154630363768078 1102 46-47 0.611258118346889 1102 48-49 0.6181792786702047 1102 50-51 0.558295718999652 1102 52-53 0.5444871092839207 1102 54-55 0.5568581188303128 1102 56-57 0.6214142168807584 1102 58-59 0.46563418025679937 1102 60-61 0.6386212348413878 1102 62-63 0.5788674981928494 1102 64-65 0.39890510210332053 1102 66-67 0.5141417055051818 1102 68-69 0.5876136165279391 1102 70-71 0.4545744115273038 1102 72-73 0.48756315358853186 1102 74-75 0.4312055683796281 1102 76-77 0.39501370599634456 1102 78-79 0.313740773912464 1102 80-81 0.5854183711882435 1102 82-83 0.5161066224507351 1102 84-85 0.7900356994373503 1102 86-87 0.7857513356954726 1102 88-89 0.7867975768660891 1102 90-91 0.9081568097847992 1102 92-93 0.9353331141715167 1102 94-95 1.18041068721136 1102 96-97 1.1595715084150484 1102 98-99 1.3519478196328691 1102 100-101 2.0267405108204386 1103 1 0.24649511360689047 1103 2 0.2690404849080039 1103 3 0.2922642048077577 1103 4 0.17111018467672778 1103 5 0.100750463281301 1103 6 0.1866560328237057 1103 7 0.20869235344045478 1103 8 0.1275143651676487 1103 9 0.12015056628231235 1103 10-11 0.19666813472601063 1103 12-13 0.19434455331406753 1103 14-15 0.23721974645689414 1103 16-17 0.2989371627331181 1103 18-19 0.22917969633829927 1103 20-21 0.30475844496459104 1103 22-23 0.34764966021704424 1103 24-25 0.24833375544950087 1103 26-27 0.2064939999922757 1103 28-29 0.253904291536422 1103 30-31 0.28406748831039863 1103 32-33 0.22396399585272064 1103 34-35 0.2890974543232403 1103 36-37 0.3787188102058181 1103 38-39 0.41763265047304543 1103 40-41 0.46091282298418434 1103 42-43 0.43543781288540373 1103 44-45 0.5210401617654128 1103 46-47 0.5027960343393332 1103 48-49 0.4293858946071367 1103 50-51 0.29349265445841155 1103 52-53 0.3046691450463328 1103 54-55 0.41570476439365933 1103 56-57 0.38201375864630194 1103 58-59 0.3417599132510958 1103 60-61 0.5037540583613591 1103 62-63 0.34490829898666675 1103 64-65 0.5290502935506609 1103 66-67 0.4981291315767393 1103 68-69 0.6717030996224835 1103 70-71 0.6546118376774928 1103 72-73 0.5466672218739532 1103 74-75 0.3067084233029753 1103 76-77 0.49751543970771195 1103 78-79 0.636210459517077 1103 80-81 0.7174027905875704 1103 82-83 0.6206572020056775 1103 84-85 0.6647161225730045 1103 86-87 0.6776576948386968 1103 88-89 0.9248349684206723 1103 90-91 1.1476684457006954 1103 92-93 1.2658712801958067 1103 94-95 1.2558438791744884 1103 96-97 1.3049121885960133 1103 98-99 1.8050970414677252 1103 100-101 2.604242676976824 1104 1 0.2026470847811197 1104 2 0.22581065588440197 1104 3 0.2214114423377822 1104 4 0.14754970707322457 1104 5 0.14463205152490843 1104 6 0.16536876420705937 1104 7 0.21108566981745014 1104 8 0.1403623233420177 1104 9 0.25292398722339016 1104 10-11 0.15980399737966877 1104 12-13 0.21811875142060444 1104 14-15 0.09345208360540624 1104 16-17 0.21206949281648946 1104 18-19 0.21492400890011965 1104 20-21 0.24038000070855503 1104 22-23 0.1481018292152072 1104 24-25 0.1803865320497522 1104 26-27 0.2299970733284411 1104 28-29 0.11783939822004896 1104 30-31 0.17036906383674477 1104 32-33 0.32592103979789755 1104 34-35 0.27298010233793235 1104 36-37 0.3854024332099186 1104 38-39 0.4648869602088155 1104 40-41 0.3605330716771391 1104 42-43 0.31032653692101064 1104 44-45 0.2825872509274916 1104 46-47 0.29071199449193585 1104 48-49 0.4623191643542057 1104 50-51 0.5468168031089675 1104 52-53 0.5263432301106263 1104 54-55 0.6269604165061367 1104 56-57 0.5678569831764761 1104 58-59 0.6124554763428023 1104 60-61 0.5789677788644028 1104 62-63 0.6654590266961478 1104 64-65 0.43613398014102955 1104 66-67 0.5406250691208996 1104 68-69 0.544368953797985 1104 70-71 0.669334707537601 1104 72-73 0.6584324472519398 1104 74-75 0.5032279584167192 1104 76-77 0.5995872921875787 1104 78-79 0.7365036628518276 1104 80-81 0.7722450613237584 1104 82-83 0.4958514933720757 1104 84-85 0.6176631693442332 1104 86-87 0.821596687349647 1104 88-89 0.7772671430709472 1104 90-91 0.8566199788361999 1104 92-93 0.9848367669839924 1104 94-95 0.808783860805125 1104 96-97 1.1314320769667923 1104 98-99 1.0108621961943065 1104 100-101 1.4142636957700958 1105 1 -0.028187438284824395 1105 2 0.014625310415802062 1105 3 -0.004642958185698376 1105 4 -0.0026380476039165046 1105 5 0.008379411805684356 1105 6 0.06784786952813704 1105 7 -0.06416900731885988 1105 8 -0.005639431513579041 1105 9 0.08342166724479938 1105 10-11 0.03885838303149569 1105 12-13 0.12114215940965778 1105 14-15 -0.00856321189443321 1105 16-17 0.09846675940581662 1105 18-19 0.19573027581662927 1105 20-21 0.26602189862948933 1105 22-23 0.18091762886423624 1105 24-25 0.037626426758414766 1105 26-27 -0.021706660944786904 1105 28-29 0.04497515414639963 1105 30-31 0.027462658613821134 1105 32-33 0.08494813595803663 1105 34-35 0.15562078821879055 1105 36-37 0.2160320991924891 1105 38-39 0.21607464796348808 1105 40-41 0.10112637645688949 1105 42-43 0.09837307033762244 1105 44-45 0.13846992098489608 1105 46-47 0.19076609014643964 1105 48-49 0.3266429872625096 1105 50-51 0.23647877033183562 1105 52-53 0.1013770631316504 1105 54-55 0.18464887758700854 1105 56-57 0.33431804917688623 1105 58-59 0.32602697927251967 1105 60-61 0.38790899120191824 1105 62-63 0.35110676174269173 1105 64-65 0.27516520318614823 1105 66-67 0.37346038782052204 1105 68-69 0.398949238441503 1105 70-71 0.3688143854174939 1105 72-73 0.19567516757554415 1105 74-75 0.17920521221643426 1105 76-77 0.12404633564256073 1105 78-79 0.3316978867000131 1105 80-81 0.5300000730723688 1105 82-83 0.39154019983529764 1105 84-85 0.5249144036918132 1105 86-87 0.6212767608156362 1105 88-89 0.6961939299006055 1105 90-91 0.8250251422757202 1105 92-93 0.8022747149926772 1105 94-95 0.8811268532800653 1105 96-97 1.2456350676571333 1105 98-99 1.3056065524002456 1105 100-101 1.8416150907315796 1106 1 -0.13723386759983214 1106 2 0.11205329900906946 1106 3 0.08680737599214439 1106 4 -2.097899840265427E-4 1106 5 0.10166789957199285 1106 6 -0.0037056071145045166 1106 7 -0.012315881814437546 1106 8 -0.15793890726258297 1106 9 0.04985416378839602 1106 10-11 -0.20125137982739005 1106 12-13 -0.2943478082369637 1106 14-15 -0.13933617214633642 1106 16-17 -0.17419990258212437 1106 18-19 -0.009092845032469654 1106 20-21 0.05014792473208729 1106 22-23 0.028492412414777846 1106 24-25 0.07972441326965907 1106 26-27 0.028000684885427063 1106 28-29 0.15419908254321513 1106 30-31 0.003217512204855666 1106 32-33 0.04682794188457251 1106 34-35 -0.3768393198129445 1106 36-37 0.017572042626717632 1106 38-39 0.042338137308867374 1106 40-41 0.013696528070923364 1106 42-43 -0.03325960540108497 1106 44-45 0.06752906473166576 1106 46-47 0.13091731819445585 1106 48-49 0.33337654798180694 1106 50-51 0.28818919476226057 1106 52-53 0.2496110203727966 1106 54-55 0.14850602913320898 1106 56-57 0.2076041741877077 1106 58-59 0.19057880432568197 1106 60-61 0.44926606671886304 1106 62-63 0.17085629796420676 1106 64-65 0.11035482016242781 1106 66-67 0.17595479089724364 1106 68-69 0.1091596172543774 1106 70-71 0.17701595419356408 1106 72-73 0.13989980947773262 1106 74-75 -0.0502598692986318 1106 76-77 -0.015761824987382766 1106 78-79 0.1248664236864947 1106 80-81 0.0734522576854495 1106 82-83 -0.046122824444939425 1106 84-85 0.4432349991997704 1106 86-87 0.41911875797564946 1106 88-89 0.5157703533438216 1106 90-91 0.6323690425066175 1106 92-93 0.46977858078816936 1106 94-95 0.1072422610191417 1106 96-97 0.6805592840293926 1106 98-99 0.9764320196292964 1106 100-101 1.6595586395903226 1107 1 -0.027310621985797923 1107 2 -0.03320406842637169 1107 3 -0.13854313159705356 1107 4 -0.023753551072140056 1107 5 -0.01056844972822546 1107 6 -0.02628223829920273 1107 7 -0.04562642291438124 1107 8 -0.11604431826199146 1107 9 0.03278770902790029 1107 10-11 -0.18194882216919694 1107 12-13 -0.09904916837889743 1107 14-15 -0.01959224596853204 1107 16-17 0.009336096826508822 1107 18-19 -0.06618892969637358 1107 20-21 0.032955188678478464 1107 22-23 0.024503771571097843 1107 24-25 -0.15873470855174787 1107 26-27 -0.17014390411516445 1107 28-29 -0.09835734363458926 1107 30-31 -0.02831123064946439 1107 32-33 -0.014441585515392319 1107 34-35 0.05378758980661047 1107 36-37 0.03425503346053915 1107 38-39 -0.03374634179619562 1107 40-41 -0.023990737846673937 1107 42-43 0.1327771948157519 1107 44-45 -0.022350875638927903 1107 46-47 0.10004000201073637 1107 48-49 0.15533686485545672 1107 50-51 0.15059186576561245 1107 52-53 0.4257949844965907 1107 54-55 0.2225392072329484 1107 56-57 0.1840850533519145 1107 58-59 0.1723434775959305 1107 60-61 0.2847400219721621 1107 62-63 0.22432102544301813 1107 64-65 0.24893159918864427 1107 66-67 0.3687539851609998 1107 68-69 0.4170780703393291 1107 70-71 0.46956966994361693 1107 72-73 0.44388044223941137 1107 74-75 0.17400646718865076 1107 76-77 0.22785326963118635 1107 78-79 0.21137834956861568 1107 80-81 0.3435248232285204 1107 82-83 0.4868720134222002 1107 84-85 0.5338834074394718 1107 86-87 0.5088108164223293 1107 88-89 0.5552394175070354 1107 90-91 0.5226013384853232 1107 92-93 0.555885482522843 1107 94-95 0.4239311039128921 1107 96-97 0.8174204854880962 1107 98-99 1.2768043954424257 1107 100-101 2.057246151910448 1108 1 -0.10258158942237827 1108 2 -0.058974373235159305 1108 3 -0.03933710168414706 1108 4 -0.011849916085395762 1108 5 -0.0348300930451515 1108 6 -0.07233250335032437 1108 7 -0.1494187837625418 1108 8 -0.02249310659974668 1108 9 -0.10179081236551468 1108 10-11 0.001723319065177975 1108 12-13 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0.7956990334409291 2316 92-93 0.9044701643527198 2316 94-95 0.8154428141174996 2316 96-97 1.2375059796329957 2316 98-99 1.2907434396452295 2316 100-101 1.8259334186714824 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 5.0 4 8.0 5 22.0 6 46.0 7 113.0 8 235.0 9 453.0 10 757.0 11 1143.0 12 1860.0 13 2504.0 14 3172.0 15 4609.0 16 6170.0 17 8583.0 18 7877.0 19 5364.0 20 4944.0 21 5241.0 22 5660.0 23 6223.0 24 7088.0 25 8454.0 26 10327.0 27 13067.0 28 16075.0 29 20133.0 30 25579.0 31 32422.0 32 42224.0 33 55650.0 34 78223.0 35 113502.0 36 178533.0 37 260982.0 38 182702.0 39 13232.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.56220689163731 19.72155540610957 14.816084940743677 22.900152761509442 2 15.881786275826087 20.58296666803183 39.096641161837844 24.43860589430424 3 19.513508893451306 24.180411792882033 29.745117905671904 26.56096140799476 4 13.444612023298012 16.999855767432997 36.40002635360484 33.15550585566416 5 14.080938195014504 37.320264463869485 34.110087626360205 14.48870971475581 6 32.87995042673253 36.16293294254024 16.868621937951506 14.088494692775729 7 28.991636291762894 32.63822732833208 20.34017518024619 18.02996119965883 8 26.775351544623955 35.29379817768384 19.22041407211272 18.710436205579487 9 26.243471695694215 16.784931436111204 19.84746960877361 37.124127259420966 10-11 24.997640640107694 26.909656993587884 27.09426577610476 20.998436590199667 12-13 25.80721416567428 23.934691878777087 27.74727048112987 22.51082347441876 14-15 22.83820311150602 25.539447607074873 25.735764156604517 25.886585124814587 16-17 22.469252643818567 28.871353453479653 26.273698212050363 22.385695690651414 18-19 22.45954810690304 27.55394030908505 28.314989681139185 21.67152190287272 20-21 23.007138634453483 26.627070898445847 28.657236926451944 21.70855354064873 22-23 22.85859027875776 26.436780585828 28.531637196013637 22.172991939400603 24-25 22.79882655128051 26.602652279010137 28.52237164848177 22.07614952122758 26-27 22.53364815739779 26.80994901025404 28.496163081714478 22.160239750633696 28-29 22.43954274989338 27.140254765841398 28.427350244713743 21.992852239551475 30-31 22.58572289644697 26.88141647443026 28.374539144556145 22.158321484566628 32-33 22.619446145956495 26.776334064587815 28.403048832604455 22.20117095685124 34-35 22.76742231473683 26.85472397269909 28.352383309621025 22.025470402943057 36-37 22.878712875694678 26.682179086990043 28.285430908148783 22.153677129166496 38-39 22.622453113244926 26.916002261426215 28.362691809452585 22.09885281587628 40-41 22.778729435730146 26.970644220992785 28.21927979871544 22.031346544561632 42-43 22.61586337693328 26.944590371208005 28.06119723263496 22.378349019223748 44-45 22.742092583062824 27.061234737612473 27.95783601291327 22.23883666641144 46-47 22.844079253124594 27.307231393553693 27.56315516753236 22.285534185789352 48-49 22.865714138174802 27.366705069329566 27.408016125557122 22.35956466693851 50-51 22.95269884061945 27.452710414837796 27.442516199454055 22.152074545088706 52-53 23.025660932383417 27.60798300548618 27.030162412993043 22.336193649137364 54-55 22.867806400720806 27.588707480328278 27.100052885274568 22.443433233676345 56-57 22.93547106178318 27.751725894019337 27.11153807116542 22.20126497303207 58-59 22.866604462662462 27.37667670359139 27.257862900712794 22.498855933033354 60-61 22.796669474156552 27.5194847514125 27.40392063291387 22.279925141517076 62-63 22.628798792007736 27.56613775456603 27.565781624770963 22.239281828655272 64-65 22.6827524559601 27.46134656236812 27.442694264351587 22.413206717320193 66-67 22.59696969157379 27.614037212002284 27.206580210223418 22.582412886200505 68-69 22.546844422918376 27.457073004827336 27.456583326359123 22.539499245895158 70-71 22.624703299364487 27.45311106085724 27.37169088646048 22.550494753317793 72-73 22.563815148966935 27.37067919769263 27.19150131478727 22.87400433855316 74-75 22.498366254565138 27.66696700279384 26.98079392015214 22.853872822488885 76-77 22.636411066377253 27.501589229210477 27.15983817462112 22.702161529791148 78-79 22.590960001282067 27.44122522894694 27.067778622596794 22.900036147174198 80-81 22.618738125297146 27.443718137512395 27.027624988203204 22.909918748987256 82-83 22.542244840451414 27.484576220558598 27.069595422657635 22.903583516332358 84-85 22.469915881992623 27.429744369577914 27.01022272397043 23.09011702445904 86-87 22.47677588573932 27.244285452275935 27.080154132975302 23.19878452900944 88-89 22.33441300016204 27.277672620563294 27.03497016522642 23.35294421404825 90-91 22.42826843419323 27.176967542713047 27.1616969822167 23.23306704087702 92-93 21.99093282239942 27.13912027721489 27.369895260921744 23.500051639463948 94-95 21.856353266511512 27.500966002069116 27.25016159389451 23.39251913752486 96-97 21.920456629623235 27.614037212002284 27.028960474934692 23.436545683439785 98-99 21.697497957811844 27.677202947873564 26.719477912093538 23.905821182221054 100-101 21.606629823431174 27.586480168007295 26.864025665900744 23.942864342660787 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 101.0 1 74.5 2 55.5 3 86.0 4 85.5 5 81.5 6 136.0 7 265.5 8 459.0 9 616.5 10 710.5 11 790.0 12 864.5 13 951.5 14 1038.5 15 1104.5 16 1135.0 17 1194.5 18 1308.0 19 1420.5 20 1541.0 21 1725.5 22 2078.0 23 2571.0 24 3084.0 25 3828.5 26 5026.5 27 6556.0 28 8104.5 29 9880.0 30 11738.0 31 13586.5 32 15998.0 33 18025.5 34 20659.5 35 23330.5 36 25964.5 37 28967.0 38 31096.5 39 32273.0 40 33330.0 41 35287.5 42 37099.0 43 39276.0 44 41815.5 45 43251.0 46 43845.5 47 45466.5 48 46640.5 49 45488.0 50 43513.5 51 41718.0 52 40518.5 53 39997.0 54 39663.5 55 37239.5 56 32729.5 57 29019.0 58 26455.5 59 23286.5 60 19027.0 61 15296.0 62 12069.5 63 8887.5 64 6405.0 65 4651.5 66 3409.5 67 2691.0 68 2045.0 69 1431.0 70 1020.5 71 702.5 72 497.5 73 349.0 74 228.0 75 134.0 76 88.5 77 56.0 78 35.0 79 23.5 80 15.0 81 9.0 82 5.0 83 2.5 84 1.0 85 0.5 86 1.0 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0463859058072305 2 0.0 3 0.0 4 0.0 5 7.122595901302189E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004318073765164451 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.7806489753255473E-4 22-23 5.787109169808029E-4 24-25 8.903244876627736E-5 26-27 0.0016916165265592697 28-29 0.003294200604352262 30-31 2.6709734629883205E-4 32-33 1.7806489753255473E-4 34-35 0.0 36-37 0.0 38-39 8.903244876627736E-5 40-41 0.0 42-43 0.008547115081562626 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 4.451622438313868E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.3354867314941603E-4 84-85 1.7806489753255473E-4 86-87 0.0 88-89 0.0 90-91 0.00979356936429051 92-93 0.001246454282727883 94-95 0.0 96-97 0.0 98-99 3.1161357068197073E-4 100-101 0.01242002660289569 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1123186.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.6005477930877 #Duplication Level Percentage of deduplicated Percentage of total 1 78.51427229300113 46.79493638238515 2 12.183747145651667 14.52316008106616 3 3.8346135984606136 6.856352131292273 4 1.75056515088785 4.173385677616203 5 0.9449011060585198 2.8158311765691124 6 0.6110337652021909 2.1850768275674106 7 0.38064873918671843 1.5880811360593612 8 0.281600840129186 1.342685146455456 9 0.21000097967732986 1.1264556083268544 >10 1.1971079042525967 12.828063949736269 >50 0.06823377928973334 2.785316694607516 >100 0.022523902569235723 2.1765366647839386 >500 3.0031825332331284E-4 0.13478046704980542 >1k 4.504773799849693E-4 0.6693380564843923 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3203 0.28517093339838634 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2661 0.23691534616706406 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1615 0.14378740475753793 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 8.903244876627735E-5 0.0 18-19 0.0 0.0 0.0 3.561297950651094E-4 0.0 20-21 0.0 0.0 0.0 3.561297950651094E-4 0.0 22-23 0.0 0.0 0.0 6.232271413639415E-4 0.0 24-25 0.0 0.0 0.0 0.001246454282727883 0.0 26-27 0.0 0.0 0.0 0.0016471003021761312 0.0 28-29 0.0 0.0 0.0 0.0025819410142220436 0.0 30-31 0.0 0.0 0.0 0.005208398252827226 0.0 32-33 0.0 0.0 0.0 0.008858728652244598 0.0 34-35 0.0 0.0 0.0 0.013310351090558465 0.0 36-37 0.0 0.0 0.0 0.019987784748029265 0.0 38-39 0.0 0.0 0.0 0.029425224317254667 0.0 40-41 0.0 0.0 0.0 0.0424684780615143 0.0 42-43 0.0 0.0 0.0 0.0629459412777581 0.0 44-45 0.0 0.0 0.0 0.08012920388964961 4.4516224383138675E-5 46-47 0.0 0.0 0.0 0.11347185595262049 8.903244876627735E-5 48-49 0.0 0.0 0.0 0.15758743431631092 8.903244876627735E-5 50-51 0.0 0.0 0.0 0.18002361140541281 8.903244876627735E-5 52-53 0.0 0.0 0.0 0.21741723988724931 8.903244876627735E-5 54-55 0.0 0.0 0.0 0.2829896384036126 8.903244876627735E-5 56-57 0.0 0.0 0.0 0.3812369456171997 8.903244876627735E-5 58-59 0.0 0.0 0.0 0.5108681910208995 8.903244876627735E-5 60-61 0.0 0.0 0.0 0.6515839762960008 8.903244876627735E-5 62-63 0.0 0.0 0.0 0.7995559061455537 8.903244876627735E-5 64-65 0.0 0.0 0.0 0.963642709221803 8.903244876627735E-5 66-67 0.0 0.0 0.0 1.176474777997589 8.903244876627735E-5 68-69 0.0 0.0 0.0 1.4334669413614485 8.903244876627735E-5 70-71 0.0 0.0 0.0 1.59247889485802 8.903244876627735E-5 72-73 0.0 0.0 0.0 1.8163064710564414 8.903244876627735E-5 74-75 0.0 0.0 0.0 2.074367023805496 8.903244876627735E-5 76-77 0.0 0.0 0.0 2.3538398804828407 8.903244876627735E-5 78-79 0.0 0.0 0.0 2.673110241758711 8.903244876627735E-5 80-81 0.0 0.0 0.0 3.1311376744368253 8.903244876627735E-5 82-83 0.0 0.0 0.0 3.6392903757703534 8.903244876627735E-5 84-85 0.0 0.0 0.0 4.287535635237619 8.903244876627735E-5 86-87 0.0 0.0 0.0 5.0375449836447395 8.903244876627735E-5 88-89 0.0 0.0 0.0 5.979908937611402 8.903244876627735E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2490 0.0 38.739025 1 GTATCAA 3850 0.0 35.42201 1 TCAACGC 5005 0.0 27.14067 4 ATCAACG 5000 0.0 26.977825 3 CAACGCA 5080 0.0 26.739971 5 TATCAAC 5130 0.0 26.294176 2 AACGCAG 5190 0.0 25.990198 6 GTACATG 3870 0.0 23.574488 1 TACATGG 3935 0.0 22.691944 2 ACGCAGA 6065 0.0 22.087893 7 CGCAGAG 6095 0.0 21.667412 8 ACATGGG 3915 0.0 21.594683 3 AAGACCG 265 1.8562787E-8 19.715391 5 GAGTACT 3460 0.0 19.633417 12-13 GCAGAGT 6620 0.0 19.590279 9 GTACTTT 3540 0.0 19.186306 14-15 AGAGTAC 5650 0.0 19.170012 10-11 GGACCAT 570 0.0 19.165121 6 AGTACTT 3680 0.0 19.040579 12-13 AGCGACC 155 9.618037E-4 18.38889 8 >>END_MODULE