FastQCFastQC Report
Thu 26 May 2016
SRR937386_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937386_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1126611
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34240.3039203416263466No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26780.2377040522416344No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16860.14965236448073027No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14130.1254203979900782No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23550.040.3706361
GTATCAA37450.038.841511
ATCAACG47100.030.4689853
TATCAAC47750.030.3527742
TATTCCG1102.720868E-630.2450685
TCAACGC47450.030.0452844
CAACGCA47950.029.8350725
TACCGCG650.005838646629.236279
AACGCAG49200.029.1619916
TCCGTTG856.4000447E-427.9464348
CGCAGAG55100.025.5220438
ACGCAGA55700.025.247127
GCAGAGT60550.022.7540769
CCGTTGC1050.002193790922.6233069
GTACATG37200.021.8514211
AGAGTAC51550.021.28869210-11
GAGTACT33950.020.56905212-13
ATTCCGT1856.5412332E-620.5444076
TCTATAC3052.0736479E-1020.2542173
TACATGG38350.020.073432
Produced by FastQC (version 0.11.5)