##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937386_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1126611 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.807154377154138 33.0 31.0 34.0 30.0 34.0 2 31.917365443795596 33.0 31.0 34.0 30.0 34.0 3 31.962367667278237 34.0 31.0 34.0 30.0 34.0 4 35.56630904544692 37.0 35.0 37.0 33.0 37.0 5 35.553682681954996 37.0 35.0 37.0 33.0 37.0 6 35.58408803038493 37.0 35.0 37.0 33.0 37.0 7 35.52537566205194 37.0 35.0 37.0 33.0 37.0 8 35.51533670450581 37.0 35.0 37.0 33.0 37.0 9 37.150770762934144 39.0 37.0 39.0 34.0 39.0 10-11 37.04154095779288 39.0 37.0 39.0 33.0 39.0 12-13 36.9044190940795 39.0 37.0 39.0 33.0 39.0 14-15 38.12415731783197 40.0 38.0 41.0 33.0 41.0 16-17 38.08408270467801 40.0 38.0 41.0 33.0 41.0 18-19 38.040903204389096 40.0 38.0 41.0 33.0 41.0 20-21 37.96057645451713 40.0 38.0 41.0 33.0 41.0 22-23 37.81327538964203 40.0 38.0 41.0 33.0 41.0 24-25 37.664130742554434 40.0 37.0 41.0 32.0 41.0 26-27 37.5090963074211 40.0 37.0 41.0 32.0 41.0 28-29 37.55603531298736 40.0 37.0 41.0 32.0 41.0 30-31 37.41708318132878 40.0 37.0 41.0 32.0 41.0 32-33 37.234628900303655 40.0 37.0 41.0 31.5 41.0 34-35 37.137222608335975 39.5 36.5 41.0 31.0 41.0 36-37 37.195761447385124 40.0 37.0 41.0 31.0 41.0 38-39 37.12342636455706 40.0 37.0 41.0 31.0 41.0 40-41 36.964720298310596 40.0 36.5 41.0 31.0 41.0 42-43 36.75015777406753 40.0 36.0 41.0 30.0 41.0 44-45 36.55843853823547 39.5 36.0 41.0 30.0 41.0 46-47 36.26039733324102 39.0 36.0 41.0 29.5 41.0 48-49 35.976127074917606 39.0 35.5 41.0 28.5 41.0 50-51 35.0982126927573 38.0 34.0 40.0 27.0 40.5 52-53 35.17466499084422 38.5 35.0 40.0 27.5 40.5 54-55 35.50452685088287 39.0 35.0 40.0 28.0 41.0 56-57 35.30506714385001 39.0 35.0 40.0 27.0 41.0 58-59 35.07221259156887 38.0 34.5 40.0 26.0 41.0 60-61 34.79092916721034 38.0 34.0 40.0 26.0 41.0 62-63 34.46492533802706 38.0 34.0 40.0 26.0 41.0 64-65 34.09863830550208 37.0 34.0 40.0 25.0 41.0 66-67 33.7104803698881 37.0 33.5 39.5 23.5 41.0 68-69 33.242489199910175 36.0 33.0 39.0 22.5 41.0 70-71 32.81188671156238 35.5 32.0 39.0 22.0 40.0 72-73 32.4158209887885 35.0 32.0 38.0 20.5 40.0 74-75 31.950498441786916 35.0 31.5 37.0 19.5 39.5 76-77 31.529342869899192 35.0 31.0 37.0 18.0 39.0 78-79 31.06741368582412 35.0 31.0 36.0 17.0 39.0 80-81 30.6078895022328 34.5 31.0 36.0 11.5 37.5 82-83 30.14638371185795 34.0 30.0 35.0 7.0 37.0 84-85 29.727349990369348 34.0 30.0 35.0 6.0 36.5 86-87 29.324126073684706 34.0 29.0 35.0 2.0 36.0 88-89 28.95196478642584 34.0 29.0 35.0 2.0 36.0 90-91 28.57001218699267 34.0 29.0 35.0 2.0 35.5 92-93 28.1481988903002 34.0 28.5 35.0 2.0 35.0 94-95 27.65256907663781 33.5 27.0 35.0 2.0 35.0 96-97 27.060040688400875 33.0 26.0 35.0 2.0 35.0 98-99 26.325514751764363 33.0 25.0 35.0 2.0 35.0 100-101 24.45191729887246 31.0 20.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.08786084149864237 1101 2 -0.009971031097627758 1101 3 0.047333864209310406 1101 4 -0.0012820742235746252 1101 5 0.06211766633426663 1101 6 0.011118981699866026 1101 7 -0.0627883077644924 1101 8 0.05449308850555212 1101 9 0.05492265294111576 1101 10-11 0.06302599458653191 1101 12-13 0.04430858186987763 1101 14-15 0.07279909470310741 1101 16-17 0.18832263393516513 1101 18-19 0.17442826900082764 1101 20-21 0.12371042694349654 1101 22-23 0.10127954355444757 1101 24-25 0.039524685252551706 1101 26-27 0.10949986717270832 1101 28-29 0.10066392703401306 1101 30-31 0.07163106115540074 1101 32-33 0.07239548331939716 1101 34-35 0.0742070992424928 1101 36-37 0.08139035297514852 1101 38-39 0.07658213668504743 1101 40-41 -0.027826333030866124 1101 42-43 0.13639564491305833 1101 44-45 0.23135527152370372 1101 46-47 0.2647017504727103 1101 48-49 0.30039610571312636 1101 50-51 0.14094228597751624 1101 52-53 0.11831881580462067 1101 54-55 0.36411976876016183 1101 56-57 0.3561117639854814 1101 58-59 0.5362226548840212 1101 60-61 0.2409456087128703 1101 62-63 0.24249304728189003 1101 64-65 0.1325760718439355 1101 66-67 0.29210785822368024 1101 68-69 0.5671542277585715 1101 70-71 0.5718873526507338 1101 72-73 0.4809304799563492 1101 74-75 0.34375812909301473 1101 76-77 0.2648615668778582 1101 78-79 0.15772557950065647 1101 80-81 0.18964514210743033 1101 82-83 0.2513619068579054 1101 84-85 0.28309793041315956 1101 86-87 0.15607744519575917 1101 88-89 0.18972776523003532 1101 90-91 0.3514601518072382 1101 92-93 0.5232500540367226 1101 94-95 0.5333813005574832 1101 96-97 0.5967624742501059 1101 98-99 0.4980385430764791 1101 100-101 0.9434767177586245 1102 1 0.09655456726035894 1102 2 0.18266578546802847 1102 3 0.17618980591230482 1102 4 0.1155782734758759 1102 5 0.09938983944657309 1102 6 0.04463271755129483 1102 7 0.09470892690762156 1102 8 0.08748108781630748 1102 9 0.15795126597864595 1102 10-11 0.345655705602816 1102 12-13 0.21628325758076272 1102 14-15 0.2920842690253025 1102 16-17 0.3250509592303601 1102 18-19 0.42719915533242414 1102 20-21 0.4157864887460221 1102 22-23 0.3475922081076632 1102 24-25 0.3789346374684257 1102 26-27 0.32192568505201535 1102 28-29 0.284725911140562 1102 30-31 0.33942017919645906 1102 32-33 0.3718661337875133 1102 34-35 0.34515341575163916 1102 36-37 0.4158776046746482 1102 38-39 0.3613103934260522 1102 40-41 0.5763781233621827 1102 42-43 0.47459130784135795 1102 44-45 0.3913151302650064 1102 46-47 0.4978360296068587 1102 48-49 0.36665720639700083 1102 50-51 0.33956879347493896 1102 52-53 0.423846090182586 1102 54-55 0.44163533704662683 1102 56-57 0.41377672482622074 1102 58-59 0.357170222043969 1102 60-61 0.41813526666451395 1102 62-63 0.2951211278200887 1102 64-65 0.3003233162433361 1102 66-67 0.18596466148612478 1102 68-69 0.21731523746918668 1102 70-71 0.21188450857817287 1102 72-73 0.18381568503529166 1102 74-75 0.16340085058327602 1102 76-77 0.277923456490786 1102 78-79 0.31032906390643333 1102 80-81 0.29235599720054495 1102 82-83 0.3832212633007934 1102 84-85 0.5373255933266385 1102 86-87 0.36625811328415736 1102 88-89 0.40587820647816386 1102 90-91 0.6125895543296629 1102 92-93 0.5635494968949502 1102 94-95 0.26198729738175786 1102 96-97 0.5127488486412233 1102 98-99 0.6495238530248031 1102 100-101 1.0303791151172454 1103 1 0.03307526072872946 1103 2 0.013160527250366982 1103 3 0.09433443358699023 1103 4 -0.03485600031400793 1103 5 -0.09211664343929726 1103 6 -0.17387588634709772 1103 7 -0.03745100106254995 1103 8 -0.16621205222893565 1103 9 -0.10462726486264984 1103 10-11 8.450262816239729E-5 1103 12-13 0.03673340371509681 1103 14-15 0.001301357578078921 1103 16-17 -0.12500528889386686 1103 18-19 -0.004514010455025641 1103 20-21 -0.2699481409630522 1103 22-23 -0.36202722826296707 1103 24-25 -0.2510151471067985 1103 26-27 -0.1754170065189271 1103 28-29 -0.2057250004114053 1103 30-31 -0.2621514951143098 1103 32-33 -0.27136817867610574 1103 34-35 -0.24526028240389763 1103 36-37 -0.09665938211787051 1103 38-39 -0.19904882915135147 1103 40-41 -0.04595600761688701 1103 42-43 0.04736263328283741 1103 44-45 0.048331743856003584 1103 46-47 0.11346092259945806 1103 48-49 0.21521253098443793 1103 50-51 0.25119075370947996 1103 52-53 0.22195334332028693 1103 54-55 0.31331886304362655 1103 56-57 0.04145316869593074 1103 58-59 0.21013802817576988 1103 60-61 0.21045203474901797 1103 62-63 -0.02243730073723782 1103 64-65 0.11066164090529185 1103 66-67 0.12073697995625565 1103 68-69 0.371583145723811 1103 70-71 0.3670716733302868 1103 72-73 0.323319090136593 1103 74-75 0.3084439657524385 1103 76-77 0.4398878404000932 1103 78-79 0.5014044958134711 1103 80-81 0.338462337879875 1103 82-83 0.289502421337378 1103 84-85 0.4407293853666978 1103 86-87 0.5853065380345406 1103 88-89 0.5794995986922551 1103 90-91 0.6729472662143827 1103 92-93 0.5810052990626957 1103 94-95 0.45210192874252897 1103 96-97 0.5761093140151381 1103 98-99 0.8088802777993571 1103 100-101 0.8019722009789767 1104 1 0.06838884012395852 1104 2 0.08893845657310351 1104 3 0.1328734417340236 1104 4 0.046643366519901974 1104 5 0.10823836205376836 1104 6 -0.039034979545661486 1104 7 0.010879006896196586 1104 8 0.04937163935351663 1104 9 0.10337399250340695 1104 10-11 0.16947502291083794 1104 12-13 0.14946966749193535 1104 14-15 0.24904202793773322 1104 16-17 0.2568791726670767 1104 18-19 0.2682377752526719 1104 20-21 0.17372842587907655 1104 22-23 0.15024052952740163 1104 24-25 0.14997083568774627 1104 26-27 0.04880646181775461 1104 28-29 -0.0050787279690851506 1104 30-31 0.16100123425083268 1104 32-33 0.04329202922178155 1104 34-35 0.21583652687149169 1104 36-37 0.26044774241147195 1104 38-39 0.19326375395004902 1104 40-41 0.1786498392407765 1104 42-43 0.30393189627328354 1104 44-45 0.3428209573534886 1104 46-47 0.6624865957570663 1104 48-49 0.5103376795187131 1104 50-51 0.3698545438348333 1104 52-53 0.2849888380679957 1104 54-55 0.465560629642944 1104 56-57 0.5474588848592532 1104 58-59 0.5817930731724914 1104 60-61 0.4963775516324773 1104 62-63 0.25011591184887294 1104 64-65 0.3608840797207691 1104 66-67 0.5645968247791373 1104 68-69 0.5998909440832207 1104 70-71 0.4778218103272778 1104 72-73 0.6028416127589011 1104 74-75 0.4551167188303289 1104 76-77 0.3923007016093507 1104 78-79 0.3695792369959534 1104 80-81 0.5525380076883728 1104 82-83 0.5624783599711627 1104 84-85 0.6927002170056973 1104 86-87 0.7091448325313969 1104 88-89 0.6085523231524022 1104 90-91 0.6273611537803312 1104 92-93 0.659657000752599 1104 94-95 0.6658757832287812 1104 96-97 0.7936633098179406 1104 98-99 0.8825005374264627 1104 100-101 1.07701535700998 1105 1 -0.08095730674059354 1105 2 -0.036417123418708286 1105 3 -0.037860761003202015 1105 4 0.016592075390612138 1105 5 0.02700455461378226 1105 6 0.04705850862617211 1105 7 -0.049258679557333096 1105 8 -0.08911380965763982 1105 9 -0.09306368179866809 1105 10-11 -0.0209322307559745 1105 12-13 0.020734933691237245 1105 14-15 -0.1650523063352125 1105 16-17 -0.15849001395072548 1105 18-19 0.009917255950611548 1105 20-21 -0.12475384275201407 1105 22-23 0.12917891266418735 1105 24-25 -0.030132286635371486 1105 26-27 0.06690537463783386 1105 28-29 0.009478261591169712 1105 30-31 -0.02109382610888133 1105 32-33 0.030947954104796338 1105 34-35 0.0680484661982419 1105 36-37 -0.04120110462574189 1105 38-39 0.11735768317459616 1105 40-41 0.054596564874493936 1105 42-43 0.10539997170830162 1105 44-45 0.22146635547856874 1105 46-47 0.3325313161011465 1105 48-49 0.3425309266117438 1105 50-51 0.4430052353424685 1105 52-53 0.40449146863234375 1105 54-55 0.47877030819953603 1105 56-57 0.40019753712820716 1105 58-59 0.5184942089582236 1105 60-61 0.4670921020212475 1105 62-63 0.43341456603804573 1105 64-65 0.3798299937574967 1105 66-67 0.3139509225938255 1105 68-69 0.3547319475831401 1105 70-71 0.30126766210656797 1105 72-73 0.4714796150001703 1105 74-75 0.35277268871872636 1105 76-77 0.3812269782994129 1105 78-79 0.4341598543797325 1105 80-81 0.6049808246049935 1105 82-83 0.5486122029653977 1105 84-85 0.5723382837546751 1105 86-87 0.5611426962761179 1105 88-89 0.657892919353646 1105 90-91 0.7394585994250811 1105 92-93 0.4992206286061247 1105 94-95 0.6328548872317086 1105 96-97 0.5797270944402051 1105 98-99 0.7100543301046862 1105 100-101 1.0548680604080367 1106 1 -0.1291526865430228 1106 2 -0.283653943218674 1106 3 -0.20159960734079263 1106 4 -0.1534891402237406 1106 5 -0.2877379287038835 1106 6 -0.11529142741845533 1106 7 -0.20989260826635814 1106 8 -0.27882508583695653 1106 9 -0.23890157419953795 1106 10-11 -0.6231962893791589 1106 12-13 -0.535134705230611 1106 14-15 -0.5332890259641161 1106 16-17 -0.5439170508907338 1106 18-19 -0.3894896157841927 1106 20-21 -0.2901191203799698 1106 22-23 -0.16350763667390567 1106 24-25 -0.18185503625394261 1106 26-27 -0.33493661707107947 1106 28-29 -0.27306770151505333 1106 30-31 -0.3427196024346131 1106 32-33 -0.1837766140038113 1106 34-35 -0.42428343905535115 1106 36-37 -0.33968062349643446 1106 38-39 -0.43171554187125594 1106 40-41 -0.4159978347926341 1106 42-43 -0.4874705456367039 1106 44-45 -0.30943757276812534 1106 46-47 -0.0983209855216245 1106 48-49 -0.12650215295490597 1106 50-51 -0.29252832966855635 1106 52-53 -0.34695889275402436 1106 54-55 -0.28419683569234877 1106 56-57 -0.3670742654885757 1106 58-59 -0.4178727022285713 1106 60-61 -0.5294937985165902 1106 62-63 -0.5262434653694399 1106 64-65 -0.35256417166345244 1106 66-67 -0.3468750648616705 1106 68-69 -0.5568751815142434 1106 70-71 -0.45802185537713314 1106 72-73 -0.3614288902934888 1106 74-75 -0.2922775123939445 1106 76-77 -0.32719316995375536 1106 78-79 -0.37850118986505876 1106 80-81 -0.4470863593553034 1106 82-83 -0.7819474835762321 1106 84-85 -0.698520028131778 1106 86-87 -0.479431784760731 1106 88-89 -0.3215533273137403 1106 90-91 -0.4729121413784405 1106 92-93 -0.25007278013803713 1106 94-95 0.14475752096007355 1106 96-97 0.1533510908032234 1106 98-99 0.38780672265195903 1106 100-101 0.5082212866867586 1107 1 0.04217939648406954 1107 2 0.05216521856926448 1107 3 0.10467262149904144 1107 4 0.09108934606090457 1107 5 0.12613772496128917 1107 6 0.15753194148101812 1107 7 0.1809474201189687 1107 8 0.2331214635712584 1107 9 0.09542123290692928 1107 10-11 -0.05181531346445212 1107 12-13 0.13960509590311432 1107 14-15 0.1549744634040522 1107 16-17 0.32250187773198746 1107 18-19 0.08346142374406895 1107 20-21 -0.27560701437427326 1107 22-23 0.06035473248744694 1107 24-25 0.04191018073331776 1107 26-27 0.22225750142077771 1107 28-29 0.13755757832544901 1107 30-31 0.20740775139360323 1107 32-33 0.21375477037242518 1107 34-35 0.11434827817269166 1107 36-37 0.08732482969733724 1107 38-39 0.1364573002944809 1107 40-41 0.26487628133378394 1107 42-43 0.020121051967436188 1107 44-45 0.05661597919984018 1107 46-47 0.14200498114250593 1107 48-49 -0.08075351097333794 1107 50-51 0.09063192495462147 1107 52-53 0.16218344362903991 1107 54-55 0.3133956911934632 1107 56-57 0.3334541578583554 1107 58-59 0.3696380689907244 1107 60-61 0.49500069337754127 1107 62-63 0.4392590648110968 1107 64-65 0.4359028092693791 1107 66-67 0.45497304405014205 1107 68-69 0.25268438121648984 1107 70-71 0.37527187788523264 1107 72-73 0.44465114620194157 1107 74-75 0.36276626944450996 1107 76-77 0.4320225585608277 1107 78-79 0.7003773994853759 1107 80-81 0.6036635075884575 1107 82-83 0.5841287724324182 1107 84-85 0.6802963627071961 1107 86-87 0.5799561776624991 1107 88-89 0.4708477886690652 1107 90-91 0.9555680184580062 1107 92-93 0.9674424896493292 1107 94-95 1.0637368317709992 1107 96-97 0.8833498869610636 1107 98-99 0.8150661443008431 1107 100-101 1.125728425259556 1108 1 0.054676008627613015 1108 2 0.0632639584087471 1108 3 0.04920211843592881 1108 4 0.06401823647992444 1108 5 0.018200007802740004 1108 6 0.15454818610012921 1108 7 0.16494208744978067 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-1.7323717440622275 2316 56-57 -1.6978920267095106 2316 58-59 -1.6552194846596322 2316 60-61 -2.156454833185826 2316 62-63 -2.0873832839117696 2316 64-65 -2.0268791990022734 2316 66-67 -1.9253036835001396 2316 68-69 -1.9715705489359792 2316 70-71 -2.287823423604017 2316 72-73 -2.2973478952805664 2316 74-75 -2.338682156989268 2316 76-77 -2.574671088598233 2316 78-79 -2.5270388633614225 2316 80-81 -2.2556447622874707 2316 82-83 -2.4550435299621007 2316 84-85 -2.751024083890954 2316 86-87 -2.792181138843528 2316 88-89 -2.621309230696543 2316 90-91 -2.7787779708563 2316 92-93 -3.25736713812638 2316 94-95 -3.1065162878484323 2316 96-97 -3.412398022938291 2316 98-99 -3.4421653376737034 2316 100-101 -3.9232882067939805 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4157.0 3 947.0 4 812.0 5 903.0 6 998.0 7 1155.0 8 1413.0 9 1731.0 10 2122.0 11 2642.0 12 3458.0 13 4188.0 14 4886.0 15 5945.0 16 7444.0 17 9470.0 18 8518.0 19 6240.0 20 6133.0 21 6668.0 22 7154.0 23 7840.0 24 9015.0 25 10183.0 26 11869.0 27 13934.0 28 16711.0 29 20349.0 30 24799.0 31 31218.0 32 39970.0 33 52763.0 34 72373.0 35 104953.0 36 159908.0 37 235918.0 38 199951.0 39 27865.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.455640891008926 18.503030249094486 14.75442078190216 23.28690807799443 2 15.195954577967255 19.464412171852146 40.82137938642534 24.518253863755255 3 19.01923559376618 23.682056683706058 30.139173817814317 27.15953390471344 4 12.722528367084395 16.001757985220824 37.48165058442333 33.79406306327145 5 13.875699398843953 36.46215617245892 34.97292262195496 14.689221806742164 6 32.44406739568688 35.399275959673574 17.73267420942035 14.423982435219198 7 28.637291210037034 31.9557011830505 21.19629924375464 18.210708363157824 8 26.299020893706814 34.574518694252255 20.10595795649464 19.0205024555463 9 25.852596311929098 16.460988411536714 20.629651990436162 37.05676328609802 10-11 24.69010745076137 26.158195054881528 27.997984742383196 21.153712751973902 12-13 25.518953369562027 23.32629034710717 28.466558709781836 22.688197573548962 14-15 22.625945469128755 24.867105232737018 26.450238045695524 26.056711252438703 16-17 22.146745956760963 28.040993719779916 27.165586617017727 22.646673706441394 18-19 22.13663791033787 26.707818271383633 29.315805666259422 21.839738152019077 20-21 22.718557012234296 25.797380823559696 29.529287126450377 21.954775037755635 22-23 22.544530619594138 25.5457376644682 29.56535269940137 22.34437901653629 24-25 22.271256604971366 25.845387433861376 29.525944656497032 22.357411304670226 26-27 22.12662405499626 26.014709294933635 29.410321223474767 22.44834542659534 28-29 22.067802849437555 26.2299236290983 29.416490388505505 22.285783132958635 30-31 22.24650858791778 25.933599811479308 29.407627084732045 22.412264515870863 32-33 22.100300100033344 25.963854618206067 29.356318772924304 22.579526508836278 34-35 22.330292639374527 26.01602989353718 29.218940636215855 22.43473683087244 36-37 22.35326126581108 25.7968446304124 29.268658096570952 22.581236007205565 38-39 22.203001846311977 26.052826840121295 29.315148516304053 22.429022797262675 40-41 22.41098007276659 26.017122910094496 29.18043339865492 22.391463618483996 42-43 22.14020778604146 26.02660433115061 29.099248532787275 22.733939350020652 44-45 22.211922808636746 26.11048054989088 29.059862118267795 22.61773452320458 46-47 22.438434486468704 26.28366151972008 28.58191469969723 22.695989294113986 48-49 22.449215507471454 26.3952202621333 28.4467852679288 22.70877896246645 50-51 22.53020206740382 26.45665979429114 28.470442190012808 22.54269594829223 52-53 22.47506007103589 26.58510167167333 28.19674842730382 22.743089829986964 54-55 22.454380182706018 26.518026291277693 28.186851515681 22.84074201033529 56-57 22.454767852244835 26.796404989484103 28.19772468926863 22.551102469002437 58-59 22.54775739200405 26.427209154481883 28.32830233974544 22.696731113768625 60-61 22.432848514000664 26.376160350026606 28.49580938095846 22.695181755014275 62-63 22.30458331869921 26.48871275578411 28.62759185154196 22.57911207397472 64-65 22.32999134458552 26.388611661737333 28.545085729502073 22.736311264175075 66-67 22.254416844890425 26.491600626446704 28.32008528068075 22.93389724798212 68-69 22.156197321346013 26.40956519883636 28.610243753245186 22.823993726572446 70-71 22.2445155281298 26.303191430241785 28.489822411811648 22.962470629816767 72-73 22.214195308300923 26.335736517266785 28.328154862435245 23.121913311997055 74-75 22.2403808450043 26.579036986387848 28.180503033369288 23.000079135238565 76-77 22.306876707395958 26.386266590498376 28.29450454675792 23.01235215534775 78-79 22.263640122164283 26.25229502585099 28.272451243203832 23.21161360878089 80-81 22.220513062181517 26.324068006391272 28.24020436416727 23.21521456725994 82-83 22.321478584898866 26.295837901230286 28.28561825985132 23.097065254019526 84-85 22.231162444782395 26.201895613665027 28.248786735238667 23.31815520631391 86-87 22.254969687109085 26.101937375585564 28.354177613358832 23.288915323946515 88-89 22.18157814021097 25.831455230017646 28.433283991447407 23.553682638323973 90-91 22.078147818027034 25.828167267182494 28.722912883342854 23.37077203144762 92-93 21.966096511096005 25.813079600249477 28.727189961515943 23.49363392713857 94-95 21.706511162074683 26.022554863511914 28.694704732573857 23.576229241839545 96-97 21.845971525006636 26.121532363422673 28.558385116083894 23.4741109954868 98-99 21.766889099996312 26.259417038265614 28.346579904183706 23.627113957554368 100-101 21.85178319715254 26.101237429336905 28.44380491851156 23.603174454998992 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1274.0 1 716.5 2 153.5 3 149.0 4 129.0 5 122.5 6 177.5 7 362.0 8 635.0 9 807.0 10 817.0 11 839.5 12 906.5 13 986.0 14 1067.0 15 1091.0 16 1170.0 17 1267.0 18 1356.0 19 1504.0 20 1642.5 21 1835.5 22 2192.5 23 2652.5 24 3184.0 25 4082.5 26 5418.0 27 6901.0 28 8268.5 29 9878.0 30 11869.0 31 13838.0 32 16114.5 33 18416.5 34 20847.5 35 23306.5 36 26274.5 37 28875.5 38 30821.0 39 32434.5 40 33605.0 41 35206.5 42 36834.0 43 38879.5 44 41461.5 45 43463.5 46 44377.0 47 45603.5 48 46278.0 49 45401.5 50 43835.0 51 41820.0 52 40303.5 53 39750.5 54 39442.5 55 36743.0 56 32578.5 57 29063.5 58 26362.0 59 23341.5 60 19028.5 61 15218.5 62 11932.5 63 8906.5 64 6411.0 65 4635.5 66 3411.0 67 2529.5 68 1873.5 69 1386.0 70 1003.5 71 670.5 72 441.5 73 320.0 74 229.5 75 138.0 76 89.5 77 59.0 78 44.0 79 31.0 80 21.5 81 19.0 82 11.5 83 9.5 84 11.5 85 7.0 86 4.0 87 5.5 88 6.5 89 6.0 90 4.0 91 5.5 92 4.0 93 3.5 94 5.0 95 4.5 96 6.0 97 6.0 98 6.5 99 5.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26104840091211606 2 0.22856158869387924 3 0.22625378236143617 4 0.23051434789825415 5 0.23166825106447567 6 0.18533460085158054 7 0.175393281265672 8 0.17876622898231953 9 0.17379556918936526 10-11 0.19429954083530163 12-13 0.18533460085158054 14-15 0.1808077499687115 16-17 0.18182851046190746 18-19 0.17654718443189354 20-21 0.17259728513213524 22-23 0.174283758990459 24-25 0.16775976801220652 26-27 0.1668721501920361 28-29 0.17468318700953567 30-31 0.18338184164720564 32-33 0.1762808990858424 34-35 0.1852458390695635 36-37 0.17086643038280294 38-39 0.1718871908759989 40-41 0.1703338596907007 42-43 0.17259728513213524 44-45 0.1732186176062545 46-47 0.17716851690601282 48-49 0.18023079838560072 50-51 0.18289365184611192 52-53 0.1871985982739384 54-55 0.17907689521937917 56-57 0.16736033999312985 58-59 0.17006757434464959 60-61 0.1683367195953173 62-63 0.1736624265163397 64-65 0.16700529286506166 66-67 0.16460872475060156 68-69 0.16571824702581459 70-71 0.17383995008037378 72-73 0.17064452592776033 74-75 0.17344052206129712 76-77 0.16940186097952176 78-79 0.16829233870430876 80-81 0.173262998497263 82-83 0.1698900507806155 84-85 0.16478624831463567 86-87 0.16349920247538857 88-89 0.16030377832277512 90-91 0.16718281642909574 92-93 0.16505253366068678 94-95 0.1635435833663971 96-97 0.15972682673966437 98-99 0.16460872475060156 100-101 0.16150206238000517 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1126611.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.363860777545895 #Duplication Level Percentage of deduplicated Percentage of total 1 80.23908197641488 50.040189372952305 2 11.38262854220753 14.197293233775014 3 3.5525234782830295 6.646472388258179 4 1.5253840396180924 3.805153515161331 5 0.8197691549902679 2.556198472576976 6 0.5279553970854886 1.975520212835602 7 0.3532039126566545 1.541901174450286 8 0.25707113628971945 1.2825558842798073 9 0.19996432509309237 1.1223492597523386 >10 1.0678755474701018 11.862354241733252 >50 0.05446269757169382 2.3016500493801755 >100 0.019367744703673015 1.7837050982014884 >500 1.4240952316997354E-4 0.06749717834040384 >1k 5.696380926798942E-4 0.8171599183027048 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3424 0.3039203416263466 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2678 0.2377040522416344 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1686 0.14965236448073027 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1413 0.1254203979900782 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 8.876178201704049E-5 14-15 0.0 0.0 0.0 0.0 8.876178201704049E-5 16-17 0.0 0.0 0.0 0.0 8.876178201704049E-5 18-19 0.0 0.0 0.0 0.0 8.876178201704049E-5 20-21 0.0 0.0 0.0 4.4380891008520246E-5 8.876178201704049E-5 22-23 0.0 0.0 0.0 3.106662370596417E-4 8.876178201704049E-5 24-25 0.0 0.0 0.0 5.769515831107632E-4 8.876178201704049E-5 26-27 0.0 0.0 0.0 8.87617820170405E-4 8.876178201704049E-5 28-29 0.0 0.0 0.0 0.0023078063324430526 8.876178201704049E-5 30-31 0.0 0.0 0.0 0.0049262789019457465 8.876178201704049E-5 32-33 0.0 0.0 0.0 0.00918684443876369 8.876178201704049E-5 34-35 0.0 0.0 0.0 0.013580552648607195 8.876178201704049E-5 36-37 0.0 0.0 0.0 0.019527592043748907 8.876178201704049E-5 38-39 0.0 0.0 0.0 0.028891960046546677 8.876178201704049E-5 40-41 0.0 0.0 0.0 0.04256127447717091 8.876178201704049E-5 42-43 0.0 0.0 0.0 0.06257705632201355 8.876178201704049E-5 44-45 0.0 0.0 0.0 0.07899798599516603 8.876178201704049E-5 46-47 0.0 0.0 0.0 0.10944327722701092 8.876178201704049E-5 48-49 0.0 0.0 0.0 0.14698951102021904 8.876178201704049E-5 50-51 0.0 0.0 0.0 0.1642092967315249 8.876178201704049E-5 52-53 0.0 0.0 0.0 0.19678487073177875 8.876178201704049E-5 54-55 0.0 0.0 0.0 0.25448002904285505 8.876178201704049E-5 56-57 0.0 0.0 0.0 0.33867057928601796 8.876178201704049E-5 58-59 0.0 0.0 0.0 0.45268508828690646 8.876178201704049E-5 60-61 0.0 0.0 0.0 0.5790374849881637 8.876178201704049E-5 62-63 0.0 0.0 0.0 0.7061443568365656 8.876178201704049E-5 64-65 0.0 0.0 0.0 0.8537552003309039 8.876178201704049E-5 66-67 0.0 0.0 0.0 1.0489867398773844 8.876178201704049E-5 68-69 0.0 0.0 0.0 1.278790993519502 8.876178201704049E-5 70-71 0.0 0.0 0.0 1.4211648918748352 8.876178201704049E-5 72-73 0.0 0.0 0.0 1.6227428988355341 8.876178201704049E-5 74-75 0.0 0.0 0.0 1.8639974223578504 8.876178201704049E-5 76-77 0.0 0.0 0.0 2.1231818258476087 8.876178201704049E-5 78-79 0.0 0.0 0.0 2.4207557000597366 8.876178201704049E-5 80-81 0.0 0.0 0.0 2.8469453964145566 8.876178201704049E-5 82-83 0.0 0.0 0.0 3.3252826397043878 8.876178201704049E-5 84-85 0.0 0.0 0.0 3.9421326438318105 8.876178201704049E-5 86-87 0.0 0.0 0.0 4.690483228017479 8.876178201704049E-5 88-89 0.0 0.0 0.0 5.633444019275508 8.876178201704049E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2355 0.0 40.370636 1 GTATCAA 3745 0.0 38.84151 1 ATCAACG 4710 0.0 30.468985 3 TATCAAC 4775 0.0 30.352774 2 TATTCCG 110 2.720868E-6 30.245068 5 TCAACGC 4745 0.0 30.045284 4 CAACGCA 4795 0.0 29.835072 5 TACCGCG 65 0.0058386466 29.23627 9 AACGCAG 4920 0.0 29.161991 6 TCCGTTG 85 6.4000447E-4 27.946434 8 CGCAGAG 5510 0.0 25.522043 8 ACGCAGA 5570 0.0 25.24712 7 GCAGAGT 6055 0.0 22.754076 9 CCGTTGC 105 0.0021937909 22.623306 9 GTACATG 3720 0.0 21.851421 1 AGAGTAC 5155 0.0 21.288692 10-11 GAGTACT 3395 0.0 20.569052 12-13 ATTCCGT 185 6.5412332E-6 20.544407 6 TCTATAC 305 2.0736479E-10 20.254217 3 TACATGG 3835 0.0 20.07343 2 >>END_MODULE