FastQCFastQC Report
Thu 26 May 2016
SRR937386_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937386_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1126611
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32830.2914049303619439No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28020.24871051321174745No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16960.15053998230090065No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27550.038.1106261
GTATCAA40250.032.341491
ATCAACG50700.025.4823763
TCAACGC51050.025.3076694
CAACGCA51500.025.0865355
TATCAAC52300.024.884442
AACGCAG52350.024.5873876
GTACATG34300.022.7156471
TAGACTG4600.021.6840535
GAGTACT34650.021.66064512-13
ACATGGG34400.021.4018973
TACATGG36250.021.2268732
ACGCAGA60650.021.1470857
CGCAGAG60850.020.8433868
GTACTTT36150.020.5620214-15
GTAAGGT3700.020.539854
CTATTAC2601.4868419E-820.099941
AGTACTT35900.019.98021112-13
AGAGTAC54000.019.4848710-11
TCTAGAC5150.019.368283
Produced by FastQC (version 0.11.5)