##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937386_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1126611 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.25681180105644 33.0 31.0 34.0 31.0 34.0 2 32.490491394101426 34.0 31.0 34.0 31.0 34.0 3 32.57311441127417 34.0 31.0 34.0 31.0 34.0 4 36.16315125628988 37.0 35.0 37.0 35.0 37.0 5 36.02021993394348 37.0 35.0 37.0 35.0 37.0 6 36.09904217161026 37.0 35.0 37.0 35.0 37.0 7 36.063979492477884 37.0 35.0 37.0 35.0 37.0 8 36.038697474105966 37.0 35.0 37.0 35.0 37.0 9 37.7599650633626 39.0 37.0 39.0 35.0 39.0 10-11 37.68673259891835 39.0 37.0 39.0 35.0 39.0 12-13 37.6022917404499 39.0 37.0 39.0 35.0 39.0 14-15 38.874864971139104 40.0 38.0 41.0 35.0 41.0 16-17 38.84482354601544 40.0 38.0 41.0 35.0 41.0 18-19 38.82671880533742 40.0 38.0 41.0 35.0 41.0 20-21 38.71579276254182 40.0 38.0 41.0 34.0 41.0 22-23 38.58925929180525 40.0 38.0 41.0 34.0 41.0 24-25 38.45203757108709 40.0 38.0 41.0 34.0 41.0 26-27 38.24229303637192 40.0 38.0 41.0 34.0 41.0 28-29 38.22617877865563 40.0 38.0 41.0 34.0 41.0 30-31 38.40243216158905 40.0 38.0 41.0 34.0 41.0 32-33 38.39505516988561 40.0 38.0 41.0 34.0 41.0 34-35 38.312378008025846 40.0 38.0 41.0 34.0 41.0 36-37 38.189203726929705 40.0 38.0 41.0 34.0 41.0 38-39 38.05765876598045 40.0 38.0 41.0 33.0 41.0 40-41 37.921427626749605 40.0 37.5 41.0 33.0 41.0 42-43 37.74398261689261 40.0 37.0 41.0 33.0 41.0 44-45 37.5115297116751 40.0 37.0 41.0 33.0 41.0 46-47 37.243289831183965 40.0 37.0 41.0 32.0 41.0 48-49 36.98067167815688 40.0 36.5 41.0 31.0 41.0 50-51 36.78338441573888 39.5 36.0 41.0 31.0 41.0 52-53 36.560020273191014 39.0 36.0 41.0 31.0 41.0 54-55 36.35778276618993 39.0 35.5 41.0 30.0 41.0 56-57 36.1136319457204 39.0 35.0 40.0 30.0 41.0 58-59 35.856507703191255 38.5 35.0 40.0 29.0 41.0 60-61 35.52797549464722 38.0 35.0 40.0 28.5 41.0 62-63 35.163345644592496 38.0 34.0 40.0 28.0 41.0 64-65 34.80547544804729 37.0 34.0 40.0 28.0 41.0 66-67 34.40880081944877 36.5 34.0 39.0 27.5 41.0 68-69 33.97449918383542 36.0 33.5 39.0 26.0 40.0 70-71 33.580890387187765 35.5 33.0 39.0 26.0 40.0 72-73 33.149954154539586 35.0 33.0 38.0 26.0 40.0 74-75 32.714937098963176 35.0 32.0 37.0 25.5 39.0 76-77 31.38668937193051 34.0 30.5 36.0 23.5 38.5 78-79 32.012289512529165 35.0 32.0 36.0 25.0 38.5 80-81 31.861141955830362 35.0 32.0 36.0 25.0 37.5 82-83 31.540588100062934 35.0 32.0 35.5 24.0 37.0 84-85 31.23287896177119 34.0 31.5 35.0 23.5 36.5 86-87 30.925476939245222 34.0 31.0 35.0 22.0 36.0 88-89 30.600468573447266 34.0 31.0 35.0 20.0 36.0 90-91 30.189321780099785 34.0 31.0 35.0 18.5 35.5 92-93 29.817560808477815 34.0 30.0 35.0 16.0 35.0 94-95 29.44943596325617 34.0 30.0 35.0 7.5 35.0 96-97 29.057844278104866 34.0 29.5 35.0 2.0 35.0 98-99 28.466910051472958 34.0 29.0 35.0 2.0 35.0 100-101 26.914817536842797 32.5 26.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06506708507605907 1101 2 0.16807038526548013 1101 3 0.08377647639274954 1101 4 0.03824440621008307 1101 5 -0.04419211802051137 1101 6 -0.03067157798775355 1101 7 0.03338850664508897 1101 8 0.04922951384666163 1101 9 0.017680855672985274 1101 10-11 0.102813956111099 1101 12-13 0.1396486841287654 1101 14-15 0.06609276431566258 1101 16-17 0.10638420032940132 1101 18-19 0.14906136133085113 1101 20-21 -0.009971005717190451 1101 22-23 0.04794476464457631 1101 24-25 -0.08478914888013378 1101 26-27 -0.0681582624413366 1101 28-29 -0.014992672258209438 1101 30-31 0.06820733133744028 1101 32-33 0.05666603073395038 1101 34-35 0.15614208591265566 1101 36-37 0.055970565257020155 1101 38-39 0.06702826199147438 1101 40-41 -0.03948281581703128 1101 42-43 0.002105009635819499 1101 44-45 0.05944103337527906 1101 46-47 -0.1006996294291369 1101 48-49 -0.10503864300199695 1101 50-51 -0.04587240692680439 1101 52-53 0.06775306581148044 1101 54-55 -0.013784880362280205 1101 56-57 -0.025229987408231125 1101 58-59 0.07536044639022066 1101 60-61 0.02663569859070236 1101 62-63 -0.025671680712385125 1101 64-65 -0.0663640346932155 1101 66-67 -0.07493399971762926 1101 68-69 -0.17235421539925255 1101 70-71 -0.3202561562794415 1101 72-73 -0.2703632171405985 1101 74-75 -0.1502065128909038 1101 76-77 -0.13202946048444986 1101 78-79 -0.14455722408416705 1101 80-81 -0.24229026524689345 1101 82-83 -0.18544886377848968 1101 84-85 -0.22265656785870647 1101 86-87 -0.17555523522414873 1101 88-89 -0.29204272838750356 1101 90-91 -0.12042090777510595 1101 92-93 -0.24848633907924622 1101 94-95 -0.4768753514038515 1101 96-97 -0.3064943902072059 1101 98-99 -0.3324225525166078 1101 100-101 -0.020138010014999708 1102 1 0.1655110197902374 1102 2 0.16359166578367024 1102 3 0.11222507649762292 1102 4 0.06171819534850442 1102 5 -0.03542829832915828 1102 6 0.07071775609164632 1102 7 0.16136667676695282 1102 8 0.19795968639941464 1102 9 0.2167219307509427 1102 10-11 0.23373231362677416 1102 12-13 0.18463343545307254 1102 14-15 0.2312043779516486 1102 16-17 0.20911960523454098 1102 18-19 0.1663861173613128 1102 20-21 0.1570339542712489 1102 22-23 0.24719821875681447 1102 24-25 0.14086948088832685 1102 26-27 0.13648016977986543 1102 28-29 0.10940632055100963 1102 30-31 0.1268318192654263 1102 32-33 0.16042673769442217 1102 34-35 0.2882179902258244 1102 36-37 0.13784534639312795 1102 38-39 0.06203978650700748 1102 40-41 0.15307942490453286 1102 42-43 0.26388544538109215 1102 44-45 0.16207823903229723 1102 46-47 0.1451537683574955 1102 48-49 0.27918884389396226 1102 50-51 0.24809260079018003 1102 52-53 0.22751583125053543 1102 54-55 0.26885201031602435 1102 56-57 0.18535482617068766 1102 58-59 0.11970435497969589 1102 60-61 0.13853513144371732 1102 62-63 0.19775615858917917 1102 64-65 0.2800692481817535 1102 66-67 0.39570164586831424 1102 68-69 0.19640874273266462 1102 70-71 0.157574205445826 1102 72-73 0.21570754375534307 1102 74-75 0.1665101489275571 1102 76-77 0.1182922346939641 1102 78-79 0.19057596559416723 1102 80-81 0.32083355474091846 1102 82-83 0.24508118982347682 1102 84-85 0.23463903411194664 1102 86-87 0.3850123934863454 1102 88-89 0.25525665332779823 1102 90-91 0.34810044315009847 1102 92-93 0.1680950208506431 1102 94-95 0.10367999313654863 1102 96-97 0.18062043250609605 1102 98-99 0.24924216560522794 1102 100-101 0.3881621887921547 1103 1 0.06950549266927908 1103 2 0.07961477736746048 1103 3 4.25485649131474E-5 1103 4 0.07144252414774854 1103 5 0.04256574349890485 1103 6 -0.023370702014830158 1103 7 0.023206277460658953 1103 8 -0.021321385024123174 1103 9 -0.020112886082607417 1103 10-11 0.057199061266558715 1103 12-13 0.04146030604310624 1103 14-15 0.11726617333940226 1103 16-17 -0.04240065516859204 1103 18-19 -0.025100222955458662 1103 20-21 -0.0442849811536874 1103 22-23 0.08488562018411727 1103 24-25 0.16160172370296522 1103 26-27 0.10233045812074693 1103 28-29 0.049290823059770617 1103 30-31 0.06461142330280012 1103 32-33 0.08920790076665241 1103 34-35 0.06813499276982782 1103 36-37 0.20778714443969903 1103 38-39 0.29380345978648137 1103 40-41 0.18601151826131712 1103 42-43 0.13494304104319355 1103 44-45 0.032928618014736344 1103 46-47 0.09581404177272645 1103 48-49 0.07433916788718875 1103 50-51 0.09564155226873794 1103 52-53 0.06641908850538414 1103 54-55 -0.059693434341255625 1103 56-57 0.0385575450146618 1103 58-59 0.07503468304171435 1103 60-61 0.08898491501181383 1103 62-63 0.3121427871461009 1103 64-65 0.11919785042846343 1103 66-67 0.1956169463652344 1103 68-69 0.14189363479870565 1103 70-71 0.21338599126585933 1103 72-73 0.29765380942374975 1103 74-75 0.06262082025924798 1103 76-77 -0.20123764781442688 1103 78-79 0.2767917005662959 1103 80-81 0.15813875091946628 1103 82-83 0.26788459230624895 1103 84-85 0.1525401884825861 1103 86-87 0.20351928526636343 1103 88-89 0.07148013311775259 1103 90-91 0.06930437457673477 1103 92-93 0.06306688168160335 1103 94-95 0.08571936783063094 1103 96-97 0.1683120638729072 1103 98-99 0.16289046829164633 1103 100-101 0.4937930943416369 1104 1 0.014700629658356945 1104 2 0.10801337084740936 1104 3 0.06089769461505057 1104 4 0.0852643209892463 1104 5 -0.028080402362945733 1104 6 0.053340758317744985 1104 7 0.008015829223374737 1104 8 0.01945704270923443 1104 9 0.0022422808005657657 1104 10-11 0.040125992159424584 1104 12-13 0.10631948262656721 1104 14-15 0.12454151804704594 1104 16-17 -0.04980653599201901 1104 18-19 0.015492157849251953 1104 20-21 -0.030227691885130525 1104 22-23 -0.20898361269073717 1104 24-25 -0.1695333286987193 1104 26-27 -0.17125359421606134 1104 28-29 -0.09562377994957671 1104 30-31 -0.0723160835312271 1104 32-33 -0.03197339042804259 1104 34-35 0.04872664573679941 1104 36-37 0.15112550407275904 1104 38-39 -0.006364338456897656 1104 40-41 0.2126457645035842 1104 42-43 0.16099303927634168 1104 44-45 0.3710474844654712 1104 46-47 0.3517129556595009 1104 48-49 0.269497253716132 1104 50-51 0.1644753557958012 1104 52-53 0.2240095394852446 1104 54-55 0.4249120064040426 1104 56-57 0.4099803939508888 1104 58-59 0.24980118162005738 1104 60-61 0.040437874278744346 1104 62-63 0.055789633947334494 1104 64-65 0.11920220163231932 1104 66-67 0.33289835111315824 1104 68-69 0.2419200321021222 1104 70-71 0.28167615801981327 1104 72-73 0.27004924472082337 1104 74-75 0.17107800467698553 1104 76-77 0.1140396399035204 1104 78-79 0.2599926254476479 1104 80-81 0.30444244279727073 1104 82-83 0.1200471669953238 1104 84-85 0.0820013723159363 1104 86-87 0.2988205388877496 1104 88-89 0.4203774589469731 1104 90-91 0.43909262126309656 1104 92-93 0.3367280689959209 1104 94-95 0.19817108924599225 1104 96-97 0.27768471521173765 1104 98-99 0.4302306749448199 1104 100-101 0.5502680285066042 1105 1 -0.04008021665277539 1105 2 -0.013329229593345815 1105 3 -0.07816142294949913 1105 4 -0.03773207708032089 1105 5 -0.07108754171609633 1105 6 -0.10599058177739096 1105 7 -0.05015812256386454 1105 8 0.011816029608169742 1105 9 0.02115384073197646 1105 10-11 -0.04798358693527405 1105 12-13 -0.1049582650642904 1105 14-15 -0.04060181658144302 1105 16-17 -0.10430789890488512 1105 18-19 -0.12667998172143058 1105 20-21 -0.06657883584683333 1105 22-23 -0.10837138953007042 1105 24-25 0.020550186281326432 1105 26-27 -0.0010368033095460305 1105 28-29 -0.1458649337674629 1105 30-31 -0.027596939593529157 1105 32-33 -0.09255092944395926 1105 34-35 -0.22603868872067068 1105 36-37 -0.21003065408813626 1105 38-39 -0.061911176304015214 1105 40-41 0.028943262054546892 1105 42-43 -0.12654302128290595 1105 44-45 0.03355170371967375 1105 46-47 0.07100901153818029 1105 48-49 -0.04007238985627026 1105 50-51 -0.06219753161424535 1105 52-53 -0.02218653094517009 1105 54-55 -0.011141802026060077 1105 56-57 0.030061943696075843 1105 58-59 -0.12099467848522494 1105 60-61 -0.047585894561628095 1105 62-63 -0.06128555653792489 1105 64-65 0.09335366273192136 1105 66-67 -0.036839132516135464 1105 68-69 -0.0013881932308095202 1105 70-71 -0.07903624711260449 1105 72-73 0.009618573255579577 1105 74-75 0.03861727561985617 1105 76-77 0.037474690392055976 1105 78-79 -0.03871887057612966 1105 80-81 0.05692951500239829 1105 82-83 0.0971872852394533 1105 84-85 -0.006191916782110951 1105 86-87 -0.05364328053866885 1105 88-89 0.0843385022916543 1105 90-91 -0.02251434076886838 1105 92-93 0.027359749481735918 1105 94-95 -0.03009573863993964 1105 96-97 0.09527701749185624 1105 98-99 0.11694373715575423 1105 100-101 0.2523403761521479 1106 1 -0.1906966539533954 1106 2 -0.08182620134510898 1106 3 -0.16196800033549863 1106 4 -6.402741553301894E-4 1106 5 -0.19966480442626278 1106 6 -0.11741547659777751 1106 7 -0.1656803990668152 1106 8 -0.1897793871805007 1106 9 -0.22720142749530226 1106 10-11 -0.3047553433192576 1106 12-13 -0.41948383950679613 1106 14-15 -0.20679928456522845 1106 16-17 -0.1228272165087958 1106 18-19 -0.18050247089524873 1106 20-21 -0.4843649783363162 1106 22-23 -0.3379765614390138 1106 24-25 -0.18361808569081006 1106 26-27 -0.1815494865070022 1106 28-29 -0.22657179471461575 1106 30-31 -0.2321553007949646 1106 32-33 -0.27690382438763095 1106 34-35 -0.2599282925055988 1106 36-37 -0.26669665294772926 1106 38-39 -0.2258020129635483 1106 40-41 -0.1836588353733717 1106 42-43 -0.4090787746463391 1106 44-45 -0.16616088238890825 1106 46-47 0.040617882070932865 1106 48-49 -0.09979726618353624 1106 50-51 0.15566720663106537 1106 52-53 0.1556827920525663 1106 54-55 0.06395239729893376 1106 56-57 0.06029568901501392 1106 58-59 -0.06348261943053046 1106 60-61 0.04894433398249731 1106 62-63 -0.1011569137787589 1106 64-65 0.003102040731270961 1106 66-67 0.06117670992249202 1106 68-69 0.19323859251100117 1106 70-71 0.09927825919817934 1106 72-73 0.028949083819767907 1106 74-75 -0.013777775929710856 1106 76-77 -0.0442645103969852 1106 78-79 -0.052634557364338974 1106 80-81 0.11088203391932083 1106 82-83 0.011654876048375229 1106 84-85 -0.08629698081453441 1106 86-87 0.021798627951213234 1106 88-89 -0.19481763357113024 1106 90-91 0.01909652547965024 1106 92-93 0.010657958731382422 1106 94-95 -0.1277688804667534 1106 96-97 0.009266869392796195 1106 98-99 -0.0018389147575135212 1106 100-101 0.0958379258138784 1107 1 -0.14907539536810077 1107 2 -0.14256743019338813 1107 3 -0.09997329724098591 1107 4 -0.12685573233343206 1107 5 -0.200549734012057 1107 6 -0.061631120632213765 1107 7 -0.11371348994211417 1107 8 -0.19765399329179445 1107 9 -0.18557911290811546 1107 10-11 -0.10173317150352545 1107 12-13 0.00883475204012285 1107 14-15 0.017001836486379318 1107 16-17 -0.0915526644647997 1107 18-19 -0.009438084347863196 1107 20-21 0.005103514791223063 1107 22-23 -0.1013962334870726 1107 24-25 -0.1283501146506083 1107 26-27 -0.049185457310663594 1107 28-29 -0.11573964410339954 1107 30-31 -0.07664793412576643 1107 32-33 -0.2059394412194564 1107 34-35 -0.25341962484205993 1107 36-37 -0.21551435322904666 1107 38-39 -0.32107503169279283 1107 40-41 -0.17100529269822573 1107 42-43 -0.19595700073211475 1107 44-45 -0.10102246166085393 1107 46-47 -0.03990648622873749 1107 48-49 -0.22593405222065854 1107 50-51 -0.1358980838539381 1107 52-53 -0.1139181870723931 1107 54-55 -0.00911290163408296 1107 56-57 0.015148968112093542 1107 58-59 0.0655608118152955 1107 60-61 -0.09404254624998742 1107 62-63 0.17364804267172929 1107 64-65 0.1257318444838873 1107 66-67 0.05050897880592231 1107 68-69 0.002965531541676114 1107 70-71 -0.1663083287329883 1107 72-73 0.0036720945234094415 1107 74-75 0.059649731652385185 1107 76-77 0.013590665279490821 1107 78-79 0.02774725067986239 1107 80-81 0.07912700101430303 1107 82-83 -0.07473294275859743 1107 84-85 -0.1858541992210725 1107 86-87 -0.2166022472631539 1107 88-89 -0.07414882533262457 1107 90-91 -0.1751639986352096 1107 92-93 -0.032721015003740916 1107 94-95 0.059011982244552286 1107 96-97 -0.10875665410535973 1107 98-99 -0.1675462037049762 1107 100-101 0.11769769298657806 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scores pass #Quality Count 2 4.0 3 1.0 4 3.0 5 23.0 6 41.0 7 81.0 8 221.0 9 477.0 10 817.0 11 1189.0 12 1809.0 13 2434.0 14 3116.0 15 4242.0 16 5843.0 17 8297.0 18 7491.0 19 4749.0 20 4449.0 21 4372.0 22 4655.0 23 5124.0 24 6030.0 25 7170.0 26 8635.0 27 10867.0 28 13860.0 29 17558.0 30 22146.0 31 28290.0 32 36728.0 33 48922.0 34 68813.0 35 102189.0 36 164771.0 37 270294.0 38 236674.0 39 24225.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.588142519940966 19.72685631672519 14.81678823949403 22.868212923839817 2 15.818592220384852 20.654866675365323 39.11678476421764 24.409756340032185 3 19.57463578821794 24.142317090814842 29.756943612302738 26.526103508664484 4 13.395395571319648 16.968589868197633 36.43458123522671 33.20143332525601 5 14.077589321459607 37.413772135628186 34.078104180925166 14.430534361987037 6 32.91011715667608 36.08423848160545 16.82701482588045 14.178629535838013 7 29.027321764122664 32.51699122412261 20.40633368571761 18.049353326037114 8 26.738066644121172 35.37618574645552 19.288023994084917 18.597723615338392 9 26.315383038156025 16.697067577007502 19.897196104067863 37.0903532807686 10-11 25.02003797229035 26.841252215715983 27.115215455911578 21.02349435608209 12-13 25.755279265476517 23.908673667073955 27.802629879539676 22.53341718790985 14-15 22.866144569864844 25.580746149291993 25.660498610434303 25.89261067040886 16-17 22.468935595338586 28.874695879944362 26.26070578043353 22.39566274428352 18-19 22.4585948477336 27.56834435310857 28.33830843121539 21.63475236794244 20-21 22.976786131149083 26.600663405558794 28.700412120953906 21.722138342338216 22-23 22.74675825841353 26.42998561592979 28.65763028101973 22.165625844636953 24-25 22.772103658198315 26.61053349150746 28.524333917832003 22.09302893246222 26-27 22.49746023475464 26.830217636467168 28.433875786062334 22.238446342715857 28-29 22.357227436618018 27.162470197464543 28.450392425939704 22.02990993997774 30-31 22.61750747937264 26.826549121093272 28.410210026020806 22.14573337351328 32-33 22.587343812549317 26.79225571204258 28.40487976772817 22.215520707679936 34-35 22.704154317683745 26.893089096413934 28.353353553267276 22.049403032635045 36-37 22.916553864834853 26.63342230254206 28.321277961170132 22.128745871452956 38-39 22.65252683948678 26.93561629852522 28.306504941838533 22.105351920149477 40-41 22.79899628176895 26.981984021104 28.22908705844342 21.989932638683626 42-43 22.588173571697105 26.90531746746851 28.08078754675493 22.42572141407945 44-45 22.70011565660197 27.11659126353284 27.965775232089868 22.21751784777532 46-47 22.86361485907736 27.300416914090135 27.552811041255588 22.283157185576922 48-49 22.882032928845895 27.42570416940719 27.313509276937648 22.37875362480927 50-51 22.984508406184567 27.499509591154357 27.33383572501955 22.18214627764153 52-53 22.990506462537784 27.555934470581395 27.06774358617035 22.38581548071047 54-55 22.842622697630325 27.543979243944893 27.1820086968794 22.431389361545378 56-57 22.94274598774555 27.751726194755776 27.105496040780714 22.200031776717964 58-59 22.870671420747712 27.404445722614106 27.25847697208708 22.4664058845511 60-61 22.825935482611122 27.527558314271737 27.396545924014585 22.24996027910255 62-63 22.639446978593323 27.522632035369792 27.558802461541738 22.279118524495146 64-65 22.61423863250048 27.508785197375136 27.46103135864997 22.415944811474414 66-67 22.587610097895368 27.61414543262936 27.248757556956214 22.54948691251905 68-69 22.573630117227687 27.452155180448262 27.455084319254823 22.51913038306922 70-71 22.609844924290638 27.401072774897457 27.40617657736344 22.582905723448466 72-73 22.545137585200216 27.387980412049945 27.183606408955708 22.88327559379413 74-75 22.507014399823895 27.770410549870363 26.91093021459936 22.811644835706378 76-77 22.603143409748352 27.55063637759617 27.215960078500924 22.630260134154558 78-79 22.637671742952982 27.441237481260167 27.032267570616654 22.888823205170198 80-81 22.635186413056505 27.49422826512434 26.983137924270224 22.887447397548932 82-83 22.524441698742997 27.458183159293437 27.196202410861968 22.821172731101594 84-85 22.44572438934113 27.476609051393957 27.07891188706661 22.998754672198302 86-87 22.41905147384501 27.2585657338691 27.12475734747841 23.19762544480748 88-89 22.317507995217515 27.3070296668504 27.052993446717633 23.32246889121445 90-91 22.355577076970672 27.2058402532031 27.246363046053514 23.19221962377272 92-93 22.031089462647156 27.219323843021513 27.301119531328016 23.44846716300332 94-95 21.821507157306293 27.543934863053888 27.349945988455644 23.28461199118418 96-97 21.97755037009225 27.61774028480105 27.114816027892502 23.289893317214194 98-99 21.661476245445897 27.787859784509866 26.740854850613953 23.809809119430284 100-101 21.35275371067396 27.858151054612602 26.856579788367302 23.93251544634614 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 99.0 1 72.0 2 54.0 3 79.5 4 91.0 5 98.0 6 158.0 7 295.0 8 489.0 9 674.5 10 780.0 11 836.0 12 888.0 13 962.0 14 1048.5 15 1121.0 16 1208.0 17 1243.0 18 1309.0 19 1430.5 20 1575.5 21 1784.5 22 2058.5 23 2553.0 24 3146.0 25 3909.0 26 5095.0 27 6646.0 28 8103.0 29 9661.0 30 11725.0 31 13635.0 32 15961.0 33 18218.5 34 20723.0 35 23163.5 36 25998.0 37 29156.0 38 31210.0 39 32617.0 40 33842.5 41 35376.0 42 36956.0 43 39247.5 44 41888.5 45 43403.0 46 43956.5 47 45749.5 48 47009.0 49 45589.0 50 43727.0 51 41987.0 52 40364.5 53 39878.5 54 39784.0 55 37342.0 56 32872.5 57 29089.5 58 26332.5 59 23226.0 60 19113.5 61 15422.0 62 12157.5 63 8940.5 64 6386.0 65 4564.5 66 3377.5 67 2674.0 68 1975.5 69 1437.5 70 1065.0 71 675.5 72 469.5 73 347.5 74 232.5 75 124.0 76 79.5 77 48.5 78 27.5 79 17.0 80 10.5 81 7.0 82 5.0 83 2.0 84 1.0 85 0.5 86 1.0 87 1.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04766507694315074 2 0.0 3 0.0 4 0.0 5 0.007988560381533644 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005103802465979828 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0015533311852982087 24-25 3.994280190766822E-4 26-27 0.0027072343515197345 28-29 0.002574091678494174 30-31 0.0011095222752130062 32-33 0.0 34-35 0.0 36-37 3.550471280681619E-4 38-39 5.325706921022429E-4 40-41 0.0 42-43 0.01113960364313858 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.219044550426012E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.3314267302556073E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.00750037058043992 92-93 0.0020859018774004516 94-95 0.0 96-97 0.0 98-99 0.001464569403281168 100-101 0.011627793444232305 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1126611.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.76747851541989 #Duplication Level Percentage of deduplicated Percentage of total 1 78.73944216702624 47.06057918033888 2 12.013701111453281 14.360572461389202 3 3.8219228577024187 6.8528007685596455 4 1.7510001433253108 4.186114537867707 5 0.9341745886347982 2.7916629827940755 6 0.5750301577167019 2.0620861558230894 7 0.38543070291228115 1.6125354878845068 8 0.2896853399188336 1.3851009863864796 9 0.2307445907615896 1.2411920133803398 >10 1.1676152745321897 12.588940910934785 >50 0.06555830433013277 2.6831353222816814 >100 0.024952140100391124 2.3448429581368746 >500 2.970486343610614E-4 0.13972278275428923 >1k 4.4557295154159214E-4 0.6907134514683129 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3283 0.2914049303619439 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2802 0.24871051321174745 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1696 0.15053998230090065 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 8.876178201704049E-5 0.0 10-11 0.0 0.0 0.0 8.876178201704049E-5 0.0 12-13 0.0 0.0 0.0 8.876178201704049E-5 0.0 14-15 0.0 0.0 0.0 8.876178201704049E-5 0.0 16-17 0.0 0.0 0.0 1.7752356403408098E-4 0.0 18-19 0.0 0.0 0.0 2.6628534605112146E-4 0.0 20-21 0.0 0.0 0.0 3.994280190766822E-4 0.0 22-23 0.0 0.0 0.0 6.657133651278037E-4 0.0 24-25 0.0 0.0 0.0 9.319987111789251E-4 0.0 26-27 0.0 0.0 0.0 0.0011982840572300466 0.0 28-29 0.0 0.0 0.0 0.002574091678494174 0.0 30-31 0.0 0.0 0.0 0.005281326030013909 0.0 32-33 0.0 0.0 0.0 0.009808176912882974 0.0 34-35 0.0 0.0 0.0 0.014157504231717959 0.0 36-37 0.0 0.0 0.0 0.01997140095383411 0.0 38-39 0.0 0.0 0.0 0.029335768956631883 0.0 40-41 0.0 0.0 0.0 0.042916321605239074 0.0 42-43 0.0 0.0 0.0 0.0631096270141158 0.0 44-45 0.0 0.0 0.0 0.08001874648836199 0.0 46-47 0.0 0.0 0.0 0.11046403772020688 0.0 48-49 0.0 0.0 0.0 0.14836531864148317 0.0 50-51 0.0 0.0 0.0 0.16523005722472087 0.0 52-53 0.0 0.0 0.0 0.1977612503339662 0.0 54-55 0.0 0.0 0.0 0.2561221220101703 0.0 56-57 0.0 0.0 0.0 0.3408452429454355 0.0 58-59 0.0 0.0 0.0 0.4547709901643069 0.0 60-61 0.0 0.0 0.0 0.5811233868655641 0.0 62-63 0.0 0.0 0.0 0.7083634013869916 0.0 64-65 0.0 0.0 0.0 0.8556635786442703 0.0 66-67 0.0 0.0 0.0 1.052182164029998 0.0 68-69 0.0 0.0 0.0 1.2827852737102692 0.0 70-71 0.0 0.0 0.0 1.4263130752318236 0.0 72-73 0.0 0.0 0.0 1.6301988885249656 0.0 74-75 0.0 0.0 0.0 1.8738943610527503 0.0 76-77 0.0 0.0 0.0 2.134143905926713 0.0 78-79 0.0 0.0 0.0 2.4338924437982588 0.0 80-81 0.0 0.0 0.0 2.8646533719269565 0.0 82-83 0.0 0.0 0.0 3.3494258444130227 0.0 84-85 0.0 0.0 0.0 3.9747082178320645 0.0 86-87 0.0 0.0 0.0 4.732156884674479 0.0 88-89 0.0 0.0 0.0 5.691449843823644 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2755 0.0 38.110626 1 GTATCAA 4025 0.0 32.34149 1 ATCAACG 5070 0.0 25.482376 3 TCAACGC 5105 0.0 25.307669 4 CAACGCA 5150 0.0 25.086535 5 TATCAAC 5230 0.0 24.88444 2 AACGCAG 5235 0.0 24.587387 6 GTACATG 3430 0.0 22.715647 1 TAGACTG 460 0.0 21.684053 5 GAGTACT 3465 0.0 21.660645 12-13 ACATGGG 3440 0.0 21.401897 3 TACATGG 3625 0.0 21.226873 2 ACGCAGA 6065 0.0 21.147085 7 CGCAGAG 6085 0.0 20.843386 8 GTACTTT 3615 0.0 20.56202 14-15 GTAAGGT 370 0.0 20.53985 4 CTATTAC 260 1.4868419E-8 20.09994 1 AGTACTT 3590 0.0 19.980211 12-13 AGAGTAC 5400 0.0 19.48487 10-11 TCTAGAC 515 0.0 19.36828 3 >>END_MODULE