FastQCFastQC Report
Thu 26 May 2016
SRR937385_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937385_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1287132
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38080.29585155213295916No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28920.22468558003374944No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18550.14411886271182756No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16510.12826967241899043No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25150.040.82671
GTATCAA40800.039.613871
ATCAACG51300.030.9413453
TCAACGC51700.030.6112254
TATCAAC52950.030.066922
CAACGCA53050.029.7414955
AACGCAG55000.028.84866
GTACATG38500.025.311721
CGCAGAG63300.024.5396358
ACGCAGA64050.024.4747857
ACATGGG37700.023.320643
TACATGG40250.023.1419682
GCAGAGT68750.021.7634669
GTATTAG3301.8189894E-1221.6075651
ACCGTGC2257.148083E-821.112598
GAGTACT38950.021.03601312-13
CATGGGG23050.020.8246384
AGAGTAC59850.020.15857310-11
AGTACTT40250.019.94355612-13
GTACTTT40500.019.8775414-15
Produced by FastQC (version 0.11.5)