FastQCFastQC Report
Thu 26 May 2016
SRR937385_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937385_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1287132
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37340.2901023360463418No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32260.25063474453280626No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19740.15336422371598252No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33000.040.1708031
GTATCAA49750.034.381881
GCGTCCG753.0797007E-431.664839
ATCAACG60900.027.534453
TCAACGC62000.027.0459374
TATCAAC63150.026.8543032
CAACGCA62800.026.7014055
AACGCAG65350.025.7291856
GTACATG43600.022.8851131
GTACCGT1050.002197104522.6177356
ACGCAGA74500.022.314147
CGCAGAG75300.021.887848
TACATGG44750.021.6548982
GAGTACT41450.021.6000712-13
TACCGTC1100.002878520921.5896577
ACATGGG44750.020.9118373
GTACTTT42700.020.800914-15
GCAGAGT81300.019.8635469
GTCTACG1456.134525E-419.6609061
AGTACTT41850.019.57771712-13
Produced by FastQC (version 0.11.5)