##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937385_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1287132 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.20385011016741 33.0 31.0 34.0 31.0 34.0 2 32.43092860716694 34.0 31.0 34.0 31.0 34.0 3 32.51711168706861 34.0 31.0 34.0 31.0 34.0 4 36.11248885118232 37.0 35.0 37.0 35.0 37.0 5 35.995844249074686 37.0 35.0 37.0 35.0 37.0 6 36.071912593269374 37.0 35.0 37.0 35.0 37.0 7 36.0229067414997 37.0 35.0 37.0 35.0 37.0 8 36.000486352604085 37.0 35.0 37.0 35.0 37.0 9 37.7145995904072 39.0 37.0 39.0 35.0 39.0 10-11 37.6353769465758 39.0 37.0 39.0 35.0 39.0 12-13 37.56096732891421 39.0 37.0 39.0 35.0 39.0 14-15 38.815125410602796 40.0 38.0 41.0 35.0 41.0 16-17 38.78590929290857 40.0 38.0 41.0 35.0 41.0 18-19 38.75951495262335 40.0 38.0 41.0 35.0 41.0 20-21 38.640653794638 40.0 38.0 41.0 34.0 41.0 22-23 38.50935607225988 40.0 38.0 41.0 34.0 41.0 24-25 38.3786348253326 40.0 38.0 41.0 34.0 41.0 26-27 38.32853157251937 40.0 38.0 41.0 34.0 41.0 28-29 38.23660821112365 40.0 38.0 41.0 34.0 41.0 30-31 38.36679532479963 40.0 38.0 41.0 34.0 41.0 32-33 38.33125273864685 40.0 38.0 41.0 34.0 41.0 34-35 38.24562709962925 40.0 38.0 41.0 34.0 41.0 36-37 38.10977040427866 40.0 38.0 41.0 33.5 41.0 38-39 37.99252368832412 40.0 38.0 41.0 33.0 41.0 40-41 37.84984329501559 40.0 37.0 41.0 33.0 41.0 42-43 37.6654519505381 40.0 37.0 41.0 33.0 41.0 44-45 37.42274957036263 40.0 37.0 41.0 32.5 41.0 46-47 37.15162625123142 40.0 37.0 41.0 31.5 41.0 48-49 36.90144289785352 40.0 36.5 41.0 31.0 41.0 50-51 36.68857156841723 39.0 36.0 41.0 31.0 41.0 52-53 36.461456944586885 39.0 36.0 41.0 30.5 41.0 54-55 36.23595909355062 39.0 35.0 40.5 30.0 41.0 56-57 35.98398998704096 39.0 35.0 40.0 29.5 41.0 58-59 35.742871748973684 38.5 35.0 40.0 29.0 41.0 60-61 35.409698072924925 38.0 34.5 40.0 28.0 41.0 62-63 35.037685334526685 38.0 34.0 40.0 28.0 41.0 64-65 34.6798832598366 37.0 34.0 40.0 27.5 41.0 66-67 34.25296162320571 36.5 34.0 39.0 26.5 41.0 68-69 33.843955398513906 36.0 33.0 39.0 26.0 40.0 70-71 33.42671575254131 35.5 33.0 38.5 26.0 40.0 72-73 32.98081898360075 35.0 32.5 38.0 25.5 40.0 74-75 32.52683563146593 35.0 32.0 37.0 25.0 39.0 76-77 31.195714192483756 34.0 30.5 36.0 23.0 38.5 78-79 31.841199659397795 34.5 31.5 36.0 24.0 38.5 80-81 31.713154517174615 35.0 32.0 36.0 24.0 37.5 82-83 31.3977995263889 34.5 31.5 35.0 23.5 37.0 84-85 31.05397193139476 34.0 31.0 35.0 22.0 36.5 86-87 30.704476697028742 34.0 31.0 35.0 20.0 36.0 88-89 30.33385076278113 34.0 31.0 35.0 19.0 36.0 90-91 29.912743603608646 34.0 30.0 35.0 17.5 35.0 92-93 29.567702069407023 34.0 30.0 35.0 9.5 35.0 94-95 29.167741148537992 34.0 29.5 35.0 4.5 35.0 96-97 28.709271077092325 34.0 29.0 35.0 2.0 35.0 98-99 28.2337910952412 34.0 29.0 35.0 2.0 35.0 100-101 26.363226149299372 32.0 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16226852832659233 1101 2 0.14615118651978776 1101 3 0.06747567280422118 1101 4 0.05991630489259592 1101 5 -0.020553222375681912 1101 6 0.01057839492572299 1101 7 0.001302588872121646 1101 8 0.08181271527477207 1101 9 0.11407685644010712 1101 10-11 0.13169054935872282 1101 12-13 0.0858581942861818 1101 14-15 0.17264787523996006 1101 16-17 0.060872870757563646 1101 18-19 -0.015703188971393445 1101 20-21 0.08395877751478764 1101 22-23 -0.0036229247276580168 1101 24-25 0.08848038305816175 1101 26-27 0.036318175309169476 1101 28-29 -0.07319041743341614 1101 30-31 0.09137368875563112 1101 32-33 0.16865150284606045 1101 34-35 -0.016380425740607052 1101 36-37 -0.03324446697536132 1101 38-39 -0.11161744551814934 1101 40-41 -0.020006027973266782 1101 42-43 0.04064385286272909 1101 44-45 0.008384845186611756 1101 46-47 0.09452867263557607 1101 48-49 -0.018767633459432886 1101 50-51 0.05693690342083357 1101 52-53 -0.011479082401812946 1101 54-55 0.015877742074543733 1101 56-57 -0.014786347593364724 1101 58-59 -0.0801622213356481 1101 60-61 -0.12328678243098778 1101 62-63 -0.15972463147422644 1101 64-65 -0.06573693384500956 1101 66-67 -0.0785534368542713 1101 68-69 0.08708117358014533 1101 70-71 -0.38753627794625345 1101 72-73 -0.15000974090355612 1101 74-75 -0.2798462517393929 1101 76-77 -0.23731311867173588 1101 78-79 -0.10927783177863759 1101 80-81 -0.10732453721479018 1101 82-83 -0.13628438283008748 1101 84-85 -0.27142230696519576 1101 86-87 -0.1761883952321348 1101 88-89 -0.18931116158180217 1101 90-91 -0.33537724040225214 1101 92-93 -0.36929874793309025 1101 94-95 -0.512508082925585 1101 96-97 -0.5322577594616789 1101 98-99 -0.488861216506411 1101 100-101 -0.17776280112554232 1102 1 0.07867763947223239 1102 2 0.06350524934911306 1102 3 0.10407277599898634 1102 4 0.10428173612466907 1102 5 -0.11968982434955677 1102 6 0.04741440283795839 1102 7 0.028488214818580104 1102 8 0.10933727596783882 1102 9 0.12971656155882982 1102 10-11 0.06793811100097713 1102 12-13 0.1511167185755653 1102 14-15 0.17566870161278558 1102 16-17 0.08999408783118668 1102 18-19 0.07308624238304873 1102 20-21 0.017760232599492554 1102 22-23 0.1474540182902544 1102 24-25 0.1080385683322973 1102 26-27 0.0909621448871647 1102 28-29 0.057389124755104604 1102 30-31 0.20572743438711427 1102 32-33 0.19123504301546745 1102 34-35 0.15634496787856733 1102 36-37 0.12335566328506786 1102 38-39 0.08636975707868544 1102 40-41 0.08002279355625319 1102 42-43 0.14584646096963638 1102 44-45 0.20682228354839083 1102 46-47 0.21478358618306004 1102 48-49 0.2707700909439481 1102 50-51 0.22356137096722506 1102 52-53 0.2739487562974503 1102 54-55 0.20008283945991678 1102 56-57 0.23063541684778954 1102 58-59 0.16909681175839353 1102 60-61 0.1567818015382798 1102 62-63 0.10603553234154361 1102 64-65 0.0588465427238134 1102 66-67 0.04565819770483159 1102 68-69 0.09379767680405138 1102 70-71 0.09937085795853307 1102 72-73 0.145248614320991 1102 74-75 0.22961071200102623 1102 76-77 0.2549609201342804 1102 78-79 0.15691073026050972 1102 80-81 0.2601184657953688 1102 82-83 0.3021738022025211 1102 84-85 0.15451441287101986 1102 86-87 0.09076739914315723 1102 88-89 0.13727561255643295 1102 90-91 0.03654265989779759 1102 92-93 -0.030018525379357897 1102 94-95 0.013323615443425751 1102 96-97 -0.1218635324623456 1102 98-99 0.003210648930856763 1102 100-101 0.48664752084636476 1103 1 0.03350142061419348 1103 2 0.10803180855345573 1103 3 0.07999155012572601 1103 4 0.1353648493767139 1103 5 0.015617833632681766 1103 6 -0.08169767393068383 1103 7 0.03929364884372433 1103 8 0.015232730932815741 1103 9 -0.0027972847226820363 1103 10-11 -0.02728285795507901 1103 12-13 0.045835974157476755 1103 14-15 -0.006333799650967364 1103 16-17 -0.0011388305441357716 1103 18-19 0.0472708972907796 1103 20-21 0.007970566527518486 1103 22-23 -0.0161733179654604 1103 24-25 -0.005973843151842573 1103 26-27 0.015002769678126526 1103 28-29 -0.22003777424514936 1103 30-31 -0.13828100589626757 1103 32-33 -0.10600869810966174 1103 34-35 -0.04431720232760483 1103 36-37 0.0018911115438839943 1103 38-39 -0.019531011166613155 1103 40-41 0.12774727012404696 1103 42-43 -7.862105815803488E-4 1103 44-45 -0.010853920491733504 1103 46-47 0.016471537018077242 1103 48-49 0.10031349599880457 1103 50-51 -0.002972981612742842 1103 52-53 -0.04745157037402947 1103 54-55 -0.019282606690516957 1103 56-57 -0.09174141030675287 1103 58-59 0.00879260423309347 1103 60-61 -0.08698578414946923 1103 62-63 -0.04853668297035085 1103 64-65 -0.08182541153879441 1103 66-67 -0.11472327465382648 1103 68-69 0.03719674098932302 1103 70-71 0.023924711759079287 1103 72-73 0.044015594591144236 1103 74-75 0.13686359366178635 1103 76-77 0.07670131791481083 1103 78-79 0.17144255194089553 1103 80-81 0.054032092602415815 1103 82-83 0.015827260322442527 1103 84-85 0.12103503136225058 1103 86-87 0.034123173853298994 1103 88-89 0.07356652375648665 1103 90-91 -0.014908733536429963 1103 92-93 0.08594223001245638 1103 94-95 0.23556024397019115 1103 96-97 0.2202005859183842 1103 98-99 0.32505170267923944 1103 100-101 0.6405031842104698 1104 1 0.07441838756184183 1104 2 0.05989768500213444 1104 3 0.05028710436280903 1104 4 0.034653014526469406 1104 5 -0.005152925093206306 1104 6 0.07279122207506106 1104 7 0.030365515423632417 1104 8 0.012337676706373202 1104 9 0.10828037613089236 1104 10-11 0.08210881735826803 1104 12-13 0.003423580150567318 1104 14-15 0.016305056970381315 1104 16-17 -0.05769755958038303 1104 18-19 -0.07480084248154384 1104 20-21 -0.16117606006671537 1104 22-23 -0.1323645873093966 1104 24-25 -0.053188843100933525 1104 26-27 -0.10045530653045631 1104 28-29 -0.06709856876410214 1104 30-31 0.046257354324282574 1104 32-33 0.02914204093791284 1104 34-35 0.04254726720478885 1104 36-37 -0.08353277289645433 1104 38-39 -0.08977558782827799 1104 40-41 0.0866984047742676 1104 42-43 0.11273793423082878 1104 44-45 0.16715639442611518 1104 46-47 0.056496338545692026 1104 48-49 0.028394388131829373 1104 50-51 -0.013056267354663476 1104 52-53 0.06852438061344657 1104 54-55 0.10343867486212588 1104 56-57 0.10953370014080122 1104 58-59 0.11532972044345513 1104 60-61 0.031530020981513474 1104 62-63 0.04631608601676618 1104 64-65 -0.04475686972421755 1104 66-67 0.11922422700531854 1104 68-69 0.10284801364691987 1104 70-71 0.031720023025414434 1104 72-73 0.08040937194849107 1104 74-75 -0.028502408206080077 1104 76-77 0.11666919486887295 1104 78-79 0.1622639272571078 1104 80-81 0.18205164040841382 1104 82-83 0.33089771720925043 1104 84-85 0.2365694698036478 1104 86-87 0.24709327595654784 1104 88-89 0.408493937983188 1104 90-91 0.4829519669412754 1104 92-93 0.41009342908463253 1104 94-95 0.18312125739421248 1104 96-97 0.2500015257754953 1104 98-99 0.6000614909290825 1104 100-101 1.0559493664227908 1105 1 0.002479176628703783 1105 2 0.10802825160251928 1105 3 0.0569863771334127 1105 4 0.046034101551491347 1105 5 -0.026620662298753928 1105 6 0.014441670807812557 1105 7 0.036555685862097675 1105 8 0.09496386101169207 1105 9 0.09412636769681626 1105 10-11 -0.08015960130565247 1105 12-13 -0.11656091872731622 1105 14-15 0.03971685844423689 1105 16-17 -0.03122263230814326 1105 18-19 0.05774611779209948 1105 20-21 0.03510135798467218 1105 22-23 0.013228883075775855 1105 24-25 -0.046009697217257894 1105 26-27 0.09350497320922813 1105 28-29 -0.04679470051785728 1105 30-31 0.031949330389728914 1105 32-33 0.014099753324323672 1105 34-35 -0.05549299395667617 1105 36-37 -0.009757902765734627 1105 38-39 0.02149619476544018 1105 40-41 0.1162835136755973 1105 42-43 -0.07132632765269875 1105 44-45 -0.12726551425473787 1105 46-47 -0.13589327216929092 1105 48-49 -0.07137489375765682 1105 50-51 -0.006562241517961809 1105 52-53 0.0232674316878132 1105 54-55 -0.07791449665267436 1105 56-57 -0.16418701563459592 1105 58-59 -0.15623124512291753 1105 60-61 -0.1010301085007157 1105 62-63 -0.24774816623074258 1105 64-65 -0.26234126427628013 1105 66-67 -0.3015912489346988 1105 68-69 -0.08078391275454067 1105 70-71 0.07870486847833291 1105 72-73 -0.06192166790827969 1105 74-75 -0.21165638869573655 1105 76-77 -0.08378045330447748 1105 78-79 0.01317290779012481 1105 80-81 -0.05021369152148836 1105 82-83 -0.03427817363149899 1105 84-85 0.02763098189217672 1105 86-87 0.2977240649880706 1105 88-89 0.16846375158749538 1105 90-91 0.13920061187788946 1105 92-93 0.1521489434016523 1105 94-95 0.09985411807073064 1105 96-97 -0.04305142761225511 1105 98-99 0.17721089046134963 1105 100-101 0.5651337652146928 1106 1 -0.16445611540308747 1106 2 -0.11642616854997101 1106 3 -0.10507508309725466 1106 4 0.09980895970097947 1106 5 -0.0240474967116171 1106 6 -0.060774585387093794 1106 7 -0.0764786366950645 1106 8 -0.060042288769984964 1106 9 -0.2383119873565107 1106 10-11 -0.19599260617255965 1106 12-13 -0.25409743537426266 1106 14-15 -0.2677482396283324 1106 16-17 -0.11876738328278691 1106 18-19 -0.2096457466921393 1106 20-21 -0.18967254419151658 1106 22-23 -5.128003768746225E-4 1106 24-25 -0.16067679051936068 1106 26-27 -0.1787377263050729 1106 28-29 -0.0967602548166937 1106 30-31 0.09422483368518897 1106 32-33 -0.021020519788294223 1106 34-35 -0.23566824418875143 1106 36-37 -0.09914041720794842 1106 38-39 0.034267068291605085 1106 40-41 -0.14899437881128108 1106 42-43 -0.25387791089383427 1106 44-45 -0.21010977468105807 1106 46-47 0.06143435466779579 1106 48-49 -1.6329032880690875E-4 1106 50-51 -0.013654890810279596 1106 52-53 0.0934661880817842 1106 54-55 -0.13663539151885828 1106 56-57 -0.13163298435008386 1106 58-59 -0.08661062260775054 1106 60-61 0.08075343204259156 1106 62-63 0.1833018267713058 1106 64-65 0.0647954695788755 1106 66-67 -0.05049642953847666 1106 68-69 0.1330907563268653 1106 70-71 0.12837715212693723 1106 72-73 0.1693538912718111 1106 74-75 -0.01804261674556784 1106 76-77 0.07278552047524656 1106 78-79 0.25088954108262485 1106 80-81 0.3090074943898493 1106 82-83 -0.026108879810411878 1106 84-85 -0.26279078878006246 1106 86-87 -0.10775549681710928 1106 88-89 -0.14045805592903804 1106 90-91 -0.0022700835593028046 1106 92-93 0.17673471715397682 1106 94-95 -0.4171565687513308 1106 96-97 -0.3752254434369995 1106 98-99 0.03446251420648139 1106 100-101 0.45004803176853514 1107 1 -0.008478295866339636 1107 2 0.026785278750175223 1107 3 0.0443161707761206 1107 4 0.02850738924443874 1107 5 0.016108053827650792 1107 6 0.0030506797025680044 1107 7 -0.008777800310426187 1107 8 -0.01630652533813759 1107 9 -0.05737161897202725 1107 10-11 -0.050297729724718465 1107 12-13 -0.05768043328403394 1107 14-15 -0.1566161594550266 1107 16-17 -0.03089642632156142 1107 18-19 0.02219850635159304 1107 20-21 -0.047531496262834594 1107 22-23 0.04928074190114984 1107 24-25 0.018882429206769302 1107 26-27 -0.013068046933838673 1107 28-29 0.018846175348265604 1107 30-31 -0.08688080348277083 1107 32-33 0.011787349204496422 1107 34-35 0.06349170667768789 1107 36-37 -0.12089323373981387 1107 38-39 -0.09833786089566843 1107 40-41 -0.09598817262033776 1107 42-43 0.01513716044657798 1107 44-45 -0.015162235610191033 1107 46-47 0.14884065349720288 1107 48-49 0.07318710001333528 1107 50-51 0.1121593602692883 1107 52-53 0.00728089942207788 1107 54-55 -0.0158176811547861 1107 56-57 0.1267667740863203 1107 58-59 0.04454792988690315 1107 60-61 0.024864366606415444 1107 62-63 0.04104534720894293 1107 64-65 0.0492979482441811 1107 66-67 0.26378469889185396 1107 68-69 0.1787544112661692 1107 70-71 0.0658139395656292 1107 72-73 0.0822510127366769 1107 74-75 0.16276619622147592 1107 76-77 -0.14180335235500507 1107 78-79 -0.2814822528495533 1107 80-81 -0.19145753019159528 1107 82-83 -0.17081397580555446 1107 84-85 -0.19948551571645723 1107 86-87 -0.2581086809712403 1107 88-89 -0.36494949713145886 1107 90-91 -0.28054570351878994 1107 92-93 -0.32529101640489344 1107 94-95 -0.5443563658848163 1107 96-97 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100-101 -1.0482701496645284 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 7.0 5 22.0 6 50.0 7 122.0 8 266.0 9 529.0 10 888.0 11 1406.0 12 2166.0 13 2805.0 14 3811.0 15 5092.0 16 6992.0 17 9484.0 18 8875.0 19 5755.0 20 5131.0 21 5266.0 22 5727.0 23 6066.0 24 7240.0 25 8617.0 26 10585.0 27 13316.0 28 16675.0 29 21347.0 30 26686.0 31 33831.0 32 43790.0 33 58416.0 34 81092.0 35 118700.0 36 189445.0 37 304257.0 38 257484.0 39 25184.0 40 2.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.457123890056195 19.77948429735106 14.867397521374896 22.89599429121785 2 15.796903503292592 20.636422682366685 39.09987476031984 24.46679905402088 3 19.54321701270732 24.106307666968114 29.72500100999742 26.625474310327146 4 13.429858009901082 16.994760444150252 36.481883753958414 33.09349779199026 5 14.08205668534629 37.30438458263013 34.11750445233899 14.496054279684593 6 32.82250771482645 36.19232526267702 16.839298533483746 14.14586848901278 7 28.87396164495949 32.59160676605041 20.444911632994906 18.08951995599519 8 26.84013760826395 35.212161612018036 19.320939888061208 18.626760891656797 9 26.317347404928164 16.736278796580304 19.880478459085783 37.06589533940575 10-11 24.974400449992697 26.931969681431273 27.07507854672248 21.018551321853547 12-13 25.803992956211736 23.933341180483282 27.763643843502965 22.499022019802013 14-15 22.863505840892774 25.51412753315122 25.711815105210654 25.910551520745344 16-17 22.433130401543895 28.845332102690325 26.317930095747755 22.403607400018025 18-19 22.476948751177037 27.504521680760014 28.401632466600162 21.616897101462786 20-21 22.969283647675606 26.526222640723716 28.712944748479565 21.79154896312111 22-23 22.766984483139048 26.490813570804804 28.552205084856375 22.189996861199766 24-25 22.743713443420503 26.535206387226168 28.651346035739163 22.069734133614162 26-27 22.52718203074408 26.726086630546973 28.548270368605 22.19846097010394 28-29 22.46956005573842 27.024353897785186 28.506153641158477 21.999932405317914 30-31 22.587815391117616 26.760619734417297 28.506827582563403 22.144737291901688 32-33 22.530750536852477 26.76625241233999 28.45893039719314 22.244066653614393 34-35 22.752367278569718 26.758949354067802 28.453530795598276 22.0351525717642 36-37 22.890348464648536 26.577344048629044 28.368380243828916 22.163927242893504 38-39 22.623359531112584 26.845537209858815 28.44137198049617 22.089731278532426 40-41 22.737955392298538 26.85653064332174 28.381238287914528 22.024275676465198 42-43 22.638454010030955 26.83484126592518 28.119151475639644 22.407553248404227 44-45 22.719348132126306 26.99311337143354 28.06409909783923 22.22343939860092 46-47 22.9046438127558 27.186566723537293 27.62750828974806 22.28128117395885 48-49 22.921425308359982 27.357644748168795 27.395364267223567 22.325565676247656 50-51 23.002419332282937 27.336395956281095 27.477135212239308 22.184049499196664 52-53 22.956697525972473 27.510892433720862 27.141233377773222 22.391176662533447 54-55 22.930204516708468 27.46773446701659 27.157121414120695 22.444939602154246 56-57 22.911286488099122 27.750611436900023 27.131444172004116 22.20665790299674 58-59 22.83406053147618 27.37881584794722 27.266356519766425 22.520767100810176 60-61 22.843461276698893 27.41669075122054 27.45258450570726 22.2872634663733 62-63 22.68737782915816 27.448971822625808 27.5939452985397 22.269705049676336 64-65 22.703498941833473 27.434288013972147 27.46621947088566 22.39599357330872 66-67 22.613609171398117 27.57370650407262 27.290946072353105 22.521738252176156 68-69 22.56858659407116 27.36405434718428 27.524954705500292 22.542404353244265 70-71 22.644414092727086 27.328626745353233 27.478883284698075 22.548075877221606 72-73 22.52549372421833 27.4163730872666 27.19891261997132 22.859220568543748 74-75 22.530672844743197 27.77722098432795 26.93216391170447 22.759942259224385 76-77 22.673043634996255 27.53664736794672 27.15863641025163 22.631672586805394 78-79 22.7546203497388 27.453050658362933 27.006592952393383 22.785736039504883 80-81 22.638431800312635 27.494460552608434 26.990510685772712 22.876596961306223 82-83 22.580185272876797 27.476394159557493 27.120019485233993 22.823401082331717 84-85 22.492448209635214 27.462878133371454 27.05836788096141 22.986305776031923 86-87 22.529624001267933 27.199424767622897 27.11252614339477 23.15842508771439 88-89 22.402869324979875 27.234425062852917 27.074495855902892 23.288209756264315 90-91 22.449919151121744 27.17971450749785 27.15007121383654 23.220295127543864 92-93 22.09515440817709 27.111905139273475 27.32901978699534 23.463920665554095 94-95 21.898220229160646 27.409387692948357 27.367317415773982 23.325074662117018 96-97 21.97964155968463 27.505104371579602 27.146399903040248 23.368854165695517 98-99 21.66703700020511 27.59259374359038 26.898995282520467 23.841373973684046 100-101 21.39370168987107 27.717731893475378 27.009847058800673 23.87871935785288 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 113.0 1 86.0 2 72.5 3 103.0 4 98.5 5 100.5 6 178.0 7 365.5 8 609.5 9 761.0 10 834.5 11 935.5 12 1042.5 13 1113.5 14 1194.5 15 1270.5 16 1365.0 17 1504.0 18 1590.0 19 1650.0 20 1733.5 21 2013.5 22 2381.5 23 2876.0 24 3599.0 25 4581.0 26 5821.5 27 7504.0 28 9319.0 29 11120.0 30 13197.0 31 15505.5 32 18379.5 33 20670.0 34 23632.0 35 26755.5 36 29678.0 37 32885.0 38 35324.5 39 37016.5 40 38246.5 41 40240.0 42 42265.0 43 44715.0 44 47593.0 45 49511.0 46 50336.5 47 52086.0 48 53500.0 49 52282.5 50 50287.5 51 48139.0 52 46444.0 53 45686.0 54 45454.0 55 42861.5 56 37634.0 57 33179.5 58 30314.0 59 26916.5 60 21814.5 61 17424.0 62 13861.5 63 10289.0 64 7350.5 65 5289.0 66 3940.5 67 3036.5 68 2246.5 69 1668.5 70 1227.5 71 797.5 72 541.0 73 388.0 74 255.5 75 152.5 76 91.0 77 46.0 78 27.0 79 25.0 80 18.5 81 10.0 82 8.0 83 4.5 84 1.5 85 1.0 86 0.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05384063173007896 2 0.0 3 0.0 4 0.0 5 0.013052274358807021 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0031465304257838355 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0012430737484578115 24-25 3.884605463930661E-5 26-27 0.0017480724587687975 28-29 0.003768067300012741 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.01674264954954115 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.1653816391791983E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.719223824751463E-4 84-85 0.0015538421855722645 86-87 0.0 88-89 0.0 90-91 0.012663813812413955 92-93 0.0018646106226867173 94-95 0.0 96-97 0.0 98-99 3.1076843711445287E-4 100-101 0.015499575801083338 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1287132.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.44102988024525 #Duplication Level Percentage of deduplicated Percentage of total 1 77.89045179657485 45.51998220829433 2 12.4112925335052 14.506574356060845 3 4.0171303458100684 7.042957037169785 4 1.839430034232574 4.299927423728255 5 0.9772878876494852 2.8556855321862664 6 0.6142333918007675 2.1537859202203826 7 0.393298009780791 1.608931851900814 8 0.29512843537734146 1.3798087768317793 9 0.2194128549081362 1.1540441888816673 >10 1.2427163324291115 13.116346955858871 >50 0.07146754524448416 2.8898218655110166 >100 0.02695576081123377 2.4731714729991454 >500 7.967145839102964E-4 0.30567178858041855 >1k 3.983572919551482E-4 0.6932906217764558 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3734 0.2901023360463418 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3226 0.25063474453280626 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1974 0.15336422371598252 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.9423027319653307E-4 0.0 18-19 0.0 0.0 0.0 3.8846054639306613E-4 0.0 20-21 0.0 0.0 0.0 3.8846054639306613E-4 0.0 22-23 0.0 0.0 0.0 7.769210927861323E-4 0.0 24-25 0.0 0.0 0.0 0.001398457967015038 0.0 26-27 0.0 0.0 0.0 0.0019034566773260242 0.0 28-29 0.0 0.0 0.0 0.0029523001525873028 0.0 30-31 0.0 0.0 0.0 0.005749216086617379 0.0 32-33 0.0 0.0 0.0 0.010022282096941105 0.0 34-35 0.0 0.0 0.0 0.014528424435100674 0.0 36-37 0.0 0.0 0.0 0.020938023450586263 0.0 38-39 0.0 0.0 0.0 0.03037761472793777 0.0 40-41 0.0 0.0 0.0 0.04269181404859797 0.0 42-43 0.0 0.0 0.0 0.06036676890948248 0.0 44-45 0.0 0.0 0.0 0.07606057498376234 0.0 46-47 0.0 0.0 0.0 0.10698203447665042 0.0 48-49 0.0 0.0 0.0 0.14396347849327032 0.0 50-51 0.0 0.0 0.0 0.16218227811910513 0.0 52-53 0.0 7.769210927861323E-5 0.0 0.19426911925117235 0.0 54-55 0.0 7.769210927861323E-5 0.0 0.25164474195342823 0.0 56-57 0.0 7.769210927861323E-5 0.0 0.3374945227062959 0.0 58-59 0.0 7.769210927861323E-5 0.0 0.4493711600674989 0.0 60-61 0.0 7.769210927861323E-5 0.0 0.5773300640493749 0.0 62-63 0.0 7.769210927861323E-5 0.0 0.7045897390477434 0.0 64-65 0.0 7.769210927861323E-5 0.0 0.8513889795296831 0.0 66-67 0.0 7.769210927861323E-5 0.0 1.0448811776880693 0.0 68-69 0.0 7.769210927861323E-5 0.0 1.280870959621857 0.0 70-71 0.0 7.769210927861323E-5 0.0 1.4188132996460348 0.0 72-73 0.0 7.769210927861323E-5 0.0 1.609158967378637 0.0 74-75 0.0 7.769210927861323E-5 0.0 1.840759145138183 0.0 76-77 0.0 7.769210927861323E-5 0.0 2.0920542725998574 0.0 78-79 0.0 7.769210927861323E-5 0.0 2.3828169915750674 0.0 80-81 0.0 7.769210927861323E-5 0.0 2.790778257397066 0.0 82-83 0.0 7.769210927861323E-5 0.0 3.2467532467532467 0.0 84-85 0.0 7.769210927861323E-5 0.0 3.8229567752180817 0.0 86-87 0.0 7.769210927861323E-5 0.0 4.537996102963799 0.0 88-89 0.0 7.769210927861323E-5 0.0 5.426405372564741 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3300 0.0 40.170803 1 GTATCAA 4975 0.0 34.38188 1 GCGTCCG 75 3.0797007E-4 31.66483 9 ATCAACG 6090 0.0 27.53445 3 TCAACGC 6200 0.0 27.045937 4 TATCAAC 6315 0.0 26.854303 2 CAACGCA 6280 0.0 26.701405 5 AACGCAG 6535 0.0 25.729185 6 GTACATG 4360 0.0 22.885113 1 GTACCGT 105 0.0021971045 22.617735 6 ACGCAGA 7450 0.0 22.31414 7 CGCAGAG 7530 0.0 21.88784 8 TACATGG 4475 0.0 21.654898 2 GAGTACT 4145 0.0 21.60007 12-13 TACCGTC 110 0.0028785209 21.589657 7 ACATGGG 4475 0.0 20.911837 3 GTACTTT 4270 0.0 20.8009 14-15 GCAGAGT 8130 0.0 19.863546 9 GTCTACG 145 6.134525E-4 19.660906 1 AGTACTT 4185 0.0 19.577717 12-13 >>END_MODULE