FastQCFastQC Report
Thu 26 May 2016
SRR937384_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937384_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1711225
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44620.2607488787272276No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34350.2007333927449634No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23020.13452351385703226No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20430.11938815760639308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29800.044.48361
GTATCAA48950.037.952121
ATCAACG62700.029.472553
TCAACGC63400.029.2995764
CAACGCA65050.028.7016165
TATCAAC64450.028.6722852
AACGCAG67000.028.0056216
GTACATG54450.025.6543751
ACGCAGA76300.024.0933197
TACATGG57050.024.0645542
CGCAGAG75850.023.986468
ACATGGG54600.023.578293
GCAGAGT82100.021.293179
GAGTACT45000.020.90049412-13
AGAGTAC72850.020.18123210-11
GTATAGG4150.019.4631351
GTACTTT47550.019.27987714-15
AGTACTT46900.019.14224212-13
CATGGGG34650.019.0583534
CAGAGTA79350.018.4682110-11
Produced by FastQC (version 0.11.5)