##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937384_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1711225 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.763659951204545 33.0 31.0 34.0 30.0 34.0 2 31.8657347806396 33.0 31.0 34.0 30.0 34.0 3 31.914711390962616 33.0 31.0 34.0 30.0 34.0 4 35.515112273371415 37.0 35.0 37.0 33.0 37.0 5 35.51736738301509 37.0 35.0 37.0 33.0 37.0 6 35.5363619629213 37.0 35.0 37.0 33.0 37.0 7 35.41522009087058 37.0 35.0 37.0 33.0 37.0 8 35.422386302210406 37.0 35.0 37.0 33.0 37.0 9 37.055286709813146 39.0 37.0 39.0 33.0 39.0 10-11 36.94336600972987 39.0 37.0 39.0 33.0 39.0 12-13 36.80220865169689 39.0 37.0 39.0 33.0 39.0 14-15 38.004545574077056 40.0 38.0 41.0 33.0 41.0 16-17 37.93069818404943 40.0 38.0 41.0 33.0 41.0 18-19 37.87170974009847 40.0 38.0 41.0 33.0 41.0 20-21 37.73463103916785 40.0 37.5 41.0 32.0 41.0 22-23 37.58440620023667 40.0 37.0 41.0 32.0 41.0 24-25 37.520155152010986 40.0 37.0 41.0 32.0 41.0 26-27 37.5329755000073 40.0 37.0 41.0 32.0 41.0 28-29 37.44715247848764 40.0 37.0 41.0 32.0 41.0 30-31 37.26005610016216 40.0 37.0 41.0 31.5 41.0 32-33 37.04996157723268 39.0 36.0 41.0 31.0 41.0 34-35 37.13737936273722 40.0 37.0 41.0 31.0 41.0 36-37 37.04504521614633 40.0 36.5 41.0 31.0 41.0 38-39 36.93708921971103 40.0 36.0 41.0 31.0 41.0 40-41 36.74159710149162 39.5 36.0 41.0 30.0 41.0 42-43 36.49835819369166 39.0 36.0 41.0 30.0 41.0 44-45 36.30962702157811 39.0 36.0 41.0 30.0 41.0 46-47 36.03597744305979 39.0 35.0 41.0 29.0 41.0 48-49 35.75758740083858 39.0 35.0 40.5 28.0 41.0 50-51 34.78253005887595 38.0 34.0 39.5 26.0 40.5 52-53 34.900150769185814 38.0 34.5 39.5 26.5 40.5 54-55 35.287215006793375 39.0 35.0 40.0 27.0 41.0 56-57 35.063881429969754 38.5 34.5 40.0 26.0 41.0 58-59 34.7917675933907 38.0 34.0 40.0 26.0 41.0 60-61 34.47316629899633 38.0 34.0 40.0 25.5 41.0 62-63 34.15386404476325 37.5 34.0 40.0 24.5 41.0 64-65 33.715021694984586 37.0 33.0 40.0 23.0 41.0 66-67 33.35575567210624 36.0 33.0 39.0 22.5 41.0 68-69 32.870046604040965 36.0 32.0 39.0 21.5 40.5 70-71 32.396930269251556 35.0 32.0 38.5 20.0 40.0 72-73 31.93487034142208 35.0 31.0 37.5 18.5 40.0 74-75 31.448077546786656 35.0 31.0 37.0 17.5 39.0 76-77 30.966993528028166 35.0 30.5 36.5 13.0 39.0 78-79 30.464810296717264 34.0 30.0 36.0 7.5 38.5 80-81 29.990417391050272 34.0 30.0 35.5 7.0 37.0 82-83 29.51964908179813 34.0 29.0 35.0 2.0 37.0 84-85 29.00859939517013 34.0 29.0 35.0 2.0 36.5 86-87 28.56148665429736 34.0 28.5 35.0 2.0 36.0 88-89 28.124230010664874 33.5 27.5 35.0 2.0 35.5 90-91 27.64124647547809 33.0 26.5 35.0 2.0 35.0 92-93 27.140512498356443 33.0 26.0 35.0 2.0 35.0 94-95 26.605527911291617 33.0 25.0 35.0 2.0 35.0 96-97 25.88160966559044 32.0 23.5 34.5 2.0 35.0 98-99 24.97014273400634 32.0 19.0 34.0 2.0 35.0 100-101 22.44038276673143 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.09362521502660925 1101 2 -0.07889422654289646 1101 3 -0.21039231358953359 1101 4 -0.1417155517511972 1101 5 -0.12297368336884773 1101 6 -0.10494966355602742 1101 7 -0.14701404234373427 1101 8 -0.20516453624637165 1101 9 -0.20109176160503495 1101 10-11 -0.20704256702804713 1101 12-13 -0.2261269334949887 1101 14-15 -0.21284404199609952 1101 16-17 -0.17720844121851798 1101 18-19 -0.05740599596386886 1101 20-21 -0.03935245068335291 1101 22-23 -0.1890637383323437 1101 24-25 -0.2008227168495509 1101 26-27 -0.14181976424020348 1101 28-29 -0.19402883159765594 1101 30-31 -0.04796897939588973 1101 32-33 -0.2776684821925315 1101 34-35 -0.20805276317597077 1101 36-37 -0.20445160806279006 1101 38-39 -0.2024145600059839 1101 40-41 -0.2687563200442611 1101 42-43 -0.21246251273888817 1101 44-45 -0.07660873837441073 1101 46-47 -0.09460925130559872 1101 48-49 -0.3047763718968852 1101 50-51 -0.32906061613675064 1101 52-53 -0.4317136541158817 1101 54-55 -0.5030674736647356 1101 56-57 -0.476066997141011 1101 58-59 -0.38624317765397365 1101 60-61 -0.453518103190234 1101 62-63 -0.34790291912411675 1101 64-65 -0.5652023972346498 1101 66-67 -0.5498132440710819 1101 68-69 -0.5628721946089357 1101 70-71 -0.5495100932475019 1101 72-73 -0.4583279118855117 1101 74-75 -0.5843184706555533 1101 76-77 -0.5438389914744022 1101 78-79 -0.6218675472700745 1101 80-81 -0.5663753742231918 1101 82-83 -0.4609748890397505 1101 84-85 -0.37383849471638086 1101 86-87 -0.3228611341932961 1101 88-89 -0.409230735478026 1101 90-91 -0.39216379708608784 1101 92-93 -0.18174398677225057 1101 94-95 -0.2687879158428963 1101 96-97 -0.3351756177114531 1101 98-99 -0.36619614058359673 1101 100-101 0.074949502116894 1102 1 -0.07879434599608004 1102 2 -0.1315780486125746 1102 3 -0.0992892682067108 1102 4 -0.2005355660048096 1102 5 -0.1384231180036295 1102 6 -0.2399023099326243 1102 7 -0.09900335173335151 1102 8 -0.17962086493986362 1102 9 -0.19049152302881112 1102 10-11 -0.25363544966006657 1102 12-13 -0.10428976290256031 1102 14-15 -0.02168440995907872 1102 16-17 -0.012975596922316868 1102 18-19 -0.0627849900534656 1102 20-21 -0.10685978904782445 1102 22-23 -0.18396755719621893 1102 24-25 -0.13105648438198614 1102 26-27 -0.22823593255849772 1102 28-29 -0.161333488459519 1102 30-31 -0.21265889648243075 1102 32-33 -0.19042646796538776 1102 34-35 0.033126059501306315 1102 36-37 -0.08911906855753671 1102 38-39 0.0023384756378064253 1102 40-41 -0.1314667737227566 1102 42-43 -0.24678133992673423 1102 44-45 0.028272488874726776 1102 46-47 0.06540539534397993 1102 48-49 -0.052207025238260485 1102 50-51 -0.05989594440097079 1102 52-53 -0.14473089735066935 1102 54-55 -0.01985009297389695 1102 56-57 -7.024718984567357E-4 1102 58-59 -0.12230342677268169 1102 60-61 0.14958783943784226 1102 62-63 0.037992849296259124 1102 64-65 -0.027133541137175143 1102 66-67 -0.11822316416823497 1102 68-69 -0.12749726056632227 1102 70-71 -0.2200627475917898 1102 72-73 -0.09696307568908935 1102 74-75 -0.26555465001899137 1102 76-77 -0.16752250085228226 1102 78-79 -0.160832233951961 1102 80-81 -0.23446893952330683 1102 82-83 -0.34836907815933316 1102 84-85 -0.2802816836700579 1102 86-87 -0.27225860423724413 1102 88-89 -0.3802562776787859 1102 90-91 -0.3076061998317847 1102 92-93 -0.2108608202724902 1102 94-95 -0.17597707325814937 1102 96-97 -0.14689832492458166 1102 98-99 -0.07836454389960323 1102 100-101 -0.015526913715874002 1103 1 0.03087176013170989 1103 2 -0.06189162012290339 1103 3 0.027294344604339216 1103 4 0.05838182033676986 1103 5 -0.02983239598521692 1103 6 -0.08228637814192297 1103 7 0.009460694832547745 1103 8 0.007925309393613134 1103 9 0.07355938602590584 1103 10-11 0.1269387792144201 1103 12-13 0.08285059757054114 1103 14-15 0.06721936329547873 1103 16-17 0.1602313685988861 1103 18-19 -0.035552934649246026 1103 20-21 0.023318239728311596 1103 22-23 -0.01878887468426882 1103 24-25 -0.09868570352282546 1103 26-27 -0.1277189609885383 1103 28-29 -0.21392189774609705 1103 30-31 -0.25064547159893635 1103 32-33 -0.16569625274831168 1103 34-35 -0.06981063941783106 1103 36-37 0.07267014077500278 1103 38-39 -0.012759202446389395 1103 40-41 0.1673144727003546 1103 42-43 0.13820730226171207 1103 44-45 0.05378153191859525 1103 46-47 0.18704934412547658 1103 48-49 0.3648277919622842 1103 50-51 0.2640294745215286 1103 52-53 0.20685569007145688 1103 54-55 0.23954685043791812 1103 56-57 0.1809308994827461 1103 58-59 0.1825505503614906 1103 60-61 0.20869664143641842 1103 62-63 0.298202099047316 1103 64-65 0.17695554246997602 1103 66-67 0.13612123833758716 1103 68-69 0.24642650705118285 1103 70-71 0.15306907224134392 1103 72-73 0.06200104484622315 1103 74-75 0.20052392585937895 1103 76-77 0.27676359927651717 1103 78-79 0.16812242664152421 1103 80-81 0.10754890090453273 1103 82-83 0.07070272555886348 1103 84-85 0.11866704579777121 1103 86-87 0.2583500305949755 1103 88-89 0.17214460669683973 1103 90-91 0.2563280320829193 1103 92-93 0.2558237619224606 1103 94-95 -0.061216578083808315 1103 96-97 -0.07522965180922725 1103 98-99 0.2807508633189997 1103 100-101 0.764211166689563 1104 1 0.10971752936803014 1104 2 0.12557869817490896 1104 3 0.11553447997008348 1104 4 0.01886692673729584 1104 5 0.06319169095235821 1104 6 0.059190445903915645 1104 7 0.05978093076041091 1104 8 0.06822014362624174 1104 9 0.15861417217675466 1104 10-11 0.11224586412935622 1104 12-13 0.10902659434309925 1104 14-15 0.10793913641215624 1104 16-17 0.23899165796927946 1104 18-19 0.22221714220875555 1104 20-21 0.19370444033985024 1104 22-23 0.061668898777419656 1104 24-25 0.1645924553491085 1104 26-27 0.12918171260998434 1104 28-29 0.2408878610133982 1104 30-31 0.05051366974678473 1104 32-33 0.12311813549762718 1104 34-35 0.23181121002626526 1104 36-37 0.20442760338207222 1104 38-39 0.22800502020056967 1104 40-41 0.3094833829772483 1104 42-43 0.2994842700855358 1104 44-45 0.37629861242808005 1104 46-47 0.37946529551036434 1104 48-49 0.43206883178233113 1104 50-51 0.2700374634096647 1104 52-53 0.552169091046423 1104 54-55 0.48424031190685213 1104 56-57 0.38709527786628684 1104 58-59 0.264966800711143 1104 60-61 0.20906785600995192 1104 62-63 0.37257843107276756 1104 64-65 0.46300656853764366 1104 66-67 0.5290683160773568 1104 68-69 0.40887459199291243 1104 70-71 0.22609808694284794 1104 72-73 0.4050768492504204 1104 74-75 0.5062715748623816 1104 76-77 0.43541465998285744 1104 78-79 0.5737875338805267 1104 80-81 0.4221076770321588 1104 82-83 0.5481384464178731 1104 84-85 0.7607320915943632 1104 86-87 0.8878913739845196 1104 88-89 0.7852336961326607 1104 90-91 0.9335555428886053 1104 92-93 1.0037062667110526 1104 94-95 1.0194915354073366 1104 96-97 1.1207508529394978 1104 98-99 1.3990527641163055 1104 100-101 1.5116681621556083 1105 1 0.15581399940900198 1105 2 0.11240692353407766 1105 3 0.10948059128992682 1105 4 0.1381691649424397 1105 5 0.07499925182759881 1105 6 0.18773480745187499 1105 7 0.16559239194517517 1105 8 0.03626390581470673 1105 9 0.15956277039120437 1105 10-11 0.02735902677941482 1105 12-13 0.029137788947075194 1105 14-15 0.11532744979665921 1105 16-17 0.06254925910001674 1105 18-19 0.12827273056507948 1105 20-21 0.19959677032750278 1105 22-23 0.25469289104837145 1105 24-25 0.23345564036333144 1105 26-27 0.20612202713953565 1105 28-29 0.01466293123686313 1105 30-31 -0.04394815910476524 1105 32-33 0.020660224846913877 1105 34-35 0.1362993391300833 1105 36-37 0.15518789536854882 1105 38-39 0.2390189217624865 1105 40-41 0.16341339210183037 1105 42-43 0.32537096430484524 1105 44-45 0.34250466685350744 1105 46-47 0.36024410502140114 1105 48-49 0.3733579914266443 1105 50-51 0.16232290165774543 1105 52-53 0.3153517793973819 1105 54-55 0.43993485326265613 1105 56-57 0.4335289994414424 1105 58-59 0.4496182776402762 1105 60-61 0.6431854557093786 1105 62-63 0.45235434218864157 1105 64-65 0.47050392956675324 1105 66-67 0.3212167067524021 1105 68-69 0.1495617084475711 1105 70-71 0.3180893917574039 1105 72-73 0.4712965905418187 1105 74-75 0.5537349423681519 1105 76-77 0.45719690462887996 1105 78-79 0.4821139790525528 1105 80-81 0.4974826792685789 1105 82-83 0.3508804713583835 1105 84-85 0.4641531818918949 1105 86-87 0.45215317778821884 1105 88-89 0.49600678041870694 1105 90-91 0.7187005521026393 1105 92-93 0.8640037994969099 1105 94-95 0.6763834538944486 1105 96-97 0.6644076614844003 1105 98-99 0.7150123654975182 1105 100-101 0.9575221152445437 1106 1 -0.2139735010292192 1106 2 -0.14877211598593476 1106 3 -0.1352738297326539 1106 4 0.09251145801454186 1106 5 0.025171685480962935 1106 6 -0.19187244648084345 1106 7 -0.5068619955222076 1106 8 -0.45499241082616493 1106 9 -0.6321518000772173 1106 10-11 -0.8008445681342167 1106 12-13 -0.37958606178738563 1106 14-15 -0.6140061038991718 1106 16-17 -0.5583749612424569 1106 18-19 -0.6696492510947536 1106 20-21 -0.6143921414323401 1106 22-23 -0.41680762730432264 1106 24-25 -0.3419726391564808 1106 26-27 -0.6692284243531716 1106 28-29 -0.41821875914381224 1106 30-31 -0.423257823502702 1106 32-33 -0.3199502794326179 1106 34-35 -0.2297025339511336 1106 36-37 -0.6100851788385242 1106 38-39 -0.4442660820688147 1106 40-41 -0.4206580053469722 1106 42-43 -0.4577133093252712 1106 44-45 -0.22178188452351577 1106 46-47 -0.13464118127455293 1106 48-49 -0.05954263009576266 1106 50-51 -0.2208485153231905 1106 52-53 -3.402339646001451E-4 1106 54-55 -0.24799789611006418 1106 56-57 -0.18878741105381636 1106 58-59 -0.2998255574684734 1106 60-61 -0.4866917923637004 1106 62-63 -0.7650902541723639 1106 64-65 -0.6463638178777913 1106 66-67 -0.593661267515273 1106 68-69 -0.6604091936864407 1106 70-71 -0.3683309697372579 1106 72-73 -0.3385397903046794 1106 74-75 -0.2428511417402035 1106 76-77 -0.14041717052474922 1106 78-79 -0.2649172873807295 1106 80-81 -0.5308355041431341 1106 82-83 -0.42729723096192984 1106 84-85 -0.5486888432394466 1106 86-87 -0.44946758837211576 1106 88-89 -0.16999268870800677 1106 90-91 0.02304910547293204 1106 92-93 -0.09100178468908027 1106 94-95 0.04781686185053502 1106 96-97 0.22887661290949168 1106 98-99 0.13423969445364747 1106 100-101 0.5330249173540125 1107 1 0.026006784291613627 1107 2 -0.019377768200985912 1107 3 0.04054440753984778 1107 4 0.02747437586054957 1107 5 -0.002957101165975473 1107 6 -0.1190266610217563 1107 7 0.03472899562272147 1107 8 -0.04742127583573108 1107 9 -0.060493856191477846 1107 10-11 0.025507210274987813 1107 12-13 -0.02457634224845151 1107 14-15 -0.09099500359775448 1107 16-17 -0.08778127471687469 1107 18-19 0.048692658032145175 1107 20-21 0.03618549591126197 1107 22-23 0.126623921143171 1107 24-25 0.13657455618696446 1107 26-27 0.11636571232118342 1107 28-29 0.17755406822229247 1107 30-31 0.07593604271492183 1107 32-33 0.103280635866831 1107 34-35 0.0470062170329939 1107 36-37 0.03166368591381996 1107 38-39 0.11206085088381457 1107 40-41 0.1355302082881238 1107 42-43 0.01938445288409696 1107 44-45 0.14657382763481763 1107 46-47 0.1065789653637026 1107 48-49 0.051913059187668864 1107 50-51 0.03995126584938902 1107 52-53 -0.01573589636771544 1107 54-55 0.023295863633592262 1107 56-57 -0.06485364355658163 1107 58-59 -0.06338674876425188 1107 60-61 0.13510089758759847 1107 62-63 0.2174408726970114 1107 64-65 0.21591017757845776 1107 66-67 0.16998237450069809 1107 68-69 0.03375398432718413 1107 70-71 0.15774676911949115 1107 72-73 0.2132922003910238 1107 74-75 0.1483519176178838 1107 76-77 0.0985549882326211 1107 78-79 0.17985623266329398 1107 80-81 0.2630142692666695 1107 82-83 0.2376565736679872 1107 84-85 0.34887291212031357 1107 86-87 0.49761891552442705 1107 88-89 0.4221418570872828 1107 90-91 0.6375088488513825 1107 92-93 0.49878842760659836 1107 94-95 0.5698243141094999 1107 96-97 0.573624433132931 1107 98-99 0.7840668924428655 1107 100-101 1.161326276343992 1108 1 0.08163194939676188 1108 2 0.19806662856091606 1108 3 0.12880400869800823 1108 4 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2315 74-75 0.5563508697875079 2315 76-77 0.22525386027404082 2315 78-79 0.35986347502029403 2315 80-81 -0.06662854384970274 2315 82-83 0.14322086114333032 2315 84-85 0.38033093726824063 2315 86-87 0.12522693450864608 2315 88-89 0.3663680035369872 2315 90-91 0.6988685628553277 2315 92-93 0.42945826858293046 2315 94-95 0.8920899391094999 2315 96-97 0.9582937501940769 2315 98-99 1.5883498990996685 2315 100-101 1.506289756373576 2316 1 0.31680033621876547 2316 2 0.4417528690430075 2316 3 0.383024127549934 2316 4 0.3494813162885464 2316 5 0.5339547049766011 2316 6 0.7977808904011781 2316 7 0.6835032982431812 2316 8 0.4726807543877527 2316 9 0.6499694322070297 2316 10-11 0.3853620989760387 2316 12-13 0.6020660774101643 2316 14-15 0.7373146759264984 2316 16-17 0.8076507224357741 2316 18-19 0.7870511281719956 2316 20-21 0.8149278399370132 2316 22-23 0.5505721052128152 2316 24-25 0.48426330468542744 2316 26-27 0.9227603972583793 2316 28-29 0.5639490497589748 2316 30-31 0.5916965979491593 2316 32-33 0.6862710260434639 2316 34-35 0.7772007862963903 2316 36-37 0.6429589358730325 2316 38-39 0.43890977793517294 2316 40-41 0.5990039814784964 2316 42-43 0.5996591040089498 2316 44-45 0.7696014410470511 2316 46-47 0.7759317111334525 2316 48-49 0.5741988031333278 2316 50-51 0.5254419897004325 2316 52-53 0.36066182871422114 2316 54-55 0.49205466893086225 2316 56-57 0.23550894931612731 2316 58-59 0.11217790250889692 2316 60-61 0.29474742514910446 2316 62-63 0.37372470357598786 2316 64-65 0.4169304798133382 2316 66-67 0.5316298363505894 2316 68-69 0.31582009635281594 2316 70-71 0.561754198936633 2316 72-73 0.1892398357516143 2316 74-75 0.4922942438476561 2316 76-77 0.03624128978531971 2316 78-79 0.29135724851653677 2316 80-81 0.2763871986315003 2316 82-83 0.2663117915944575 2316 84-85 0.5510017549374169 2316 86-87 0.6314093828169156 2316 88-89 0.8539443663189452 2316 90-91 1.1827736380432974 2316 92-93 1.3493801435829305 2316 94-95 1.6299746900493481 2316 96-97 1.657747462600092 2316 98-99 1.6636994056786136 2316 100-101 2.0374663306216974 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6716.0 3 1659.0 4 1298.0 5 1396.0 6 1671.0 7 1897.0 8 2282.0 9 3038.0 10 3602.0 11 4515.0 12 5786.0 13 7109.0 14 7859.0 15 9512.0 16 11442.0 17 14590.0 18 13098.0 19 10264.0 20 10546.0 21 11467.0 22 12440.0 23 13998.0 24 15734.0 25 17750.0 26 20854.0 27 24624.0 28 29213.0 29 34999.0 30 42618.0 31 53137.0 32 67540.0 33 87371.0 34 119072.0 35 167630.0 36 247030.0 37 340775.0 38 257398.0 39 29292.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.50694796246396 18.45978779241209 14.528612064921443 23.50465218020251 2 15.259332473371131 19.61088564591144 40.376279330400564 24.753502550316863 3 19.117857151221475 23.612474032924027 29.77355070342379 27.496118112430707 4 12.836982877191586 16.03318579795871 37.06362250774224 34.066208817107466 5 14.020369374971455 36.58893186670086 34.6433954110939 14.747303347233787 6 32.766341971630716 35.28661808637048 17.561818065756164 14.385221876242642 7 29.043625025767884 31.753820674275218 20.872043721069694 18.330510578887203 8 26.515409705508613 34.51850160157874 19.926274675145958 19.03981401776669 9 25.977437401260815 16.478039937181677 20.407991183886466 37.136531477671035 10-11 24.84342630590323 26.170507135087178 27.72486834457486 21.261198214434735 12-13 25.67840242170185 23.289232381067833 28.19444944671035 22.837915750519965 14-15 22.81730149314561 24.78581303795608 26.124953666753136 26.271931802145176 16-17 22.39252791811584 28.07304242312087 26.728481133859965 22.80594852490333 18-19 22.42192497946839 26.85080947982193 28.720101069693293 22.00716447101639 20-21 22.957159825263076 25.928893747571834 28.963895294861974 22.150051132303116 22-23 22.838098717851878 25.68737531743369 28.91525947733888 22.559266487375552 24-25 22.5805299485544 25.920468780601908 28.95892987993294 22.54007139091076 26-27 22.35169017008124 26.113661335933948 28.88321622858394 22.651432265400867 28-29 22.391445208088545 26.28071099029704 28.798784545883894 22.529059255730527 30-31 22.48196156935152 26.05240404562113 28.820321917509407 22.645312467517943 32-33 22.339264638410768 26.051822622424847 28.834674667335797 22.77423807182859 34-35 22.635064700811455 26.086584771649708 28.6708398924308 22.60751063510804 36-37 22.628932018142237 25.981884698665798 28.66264247545727 22.72654080773469 38-39 22.461197047125662 26.13173754375076 28.765375894228196 22.641689514895386 40-41 22.64417245626367 26.125864487274107 28.58740078688359 22.642562269578626 42-43 22.407470298566018 26.04758670359462 28.559282832600434 22.98566016523893 44-45 22.500715175212804 26.209231718818582 28.464383395988634 22.82566970997998 46-47 22.72720617861192 26.338570411251556 28.103068223344547 22.831155186791978 48-49 22.74638335552924 26.442675409160177 27.91955242743578 22.891388807874804 50-51 22.816377625346814 26.494154508385737 27.968931413386432 22.720536452881017 52-53 22.748398637377466 26.533506784998035 27.80197458437201 22.916119993252487 54-55 22.72395319064703 26.50100555073375 27.770037516354108 23.005003742265107 56-57 22.70001873711823 26.75836143901068 27.806556820311034 22.73506300356005 58-59 22.817694097644754 26.474373967565718 27.82378734437192 22.884144590417606 60-61 22.714557700143786 26.434219400587327 27.984476064240855 22.86674683502803 62-63 22.535102415650424 26.45564184075039 28.21504403043034 22.794211713168846 64-65 22.583107859090966 26.494489529535297 28.016678507540544 22.905724103833194 66-67 22.52131574348453 26.56468402760194 27.846394919173324 23.067605309740213 68-69 22.37926562586908 26.491684645942442 28.14746616627195 22.981583561916526 70-71 22.51095443517035 26.37969676530928 28.038465231524416 23.070883567995953 72-73 22.470919382248198 26.38621636535824 27.910367146915853 23.23249710547771 74-75 22.435810737623957 26.6980082051246 27.649115288031258 23.217065769220184 76-77 22.594060675580366 26.480329753624577 27.804473596610418 23.12113597418464 78-79 22.56599096006626 26.335079469033438 27.839353954187875 23.25957561671243 80-81 22.507125509675937 26.47188321892704 27.70968436978175 23.31130690161527 82-83 22.59788034360179 26.43519438770865 27.76139213584818 23.20553313284138 84-85 22.52771895146629 26.333808953088123 27.727060617220555 23.411411478225027 86-87 22.55374511966911 26.247397352032582 27.875033082027034 23.323824446271274 88-89 22.533175639873416 26.09120263707182 27.822366514340246 23.553255208714514 90-91 22.47227196022105 26.089828192135318 28.047068867187637 23.39083098045599 92-93 22.33940901322457 26.009963951083076 28.096374791637025 23.554252244055323 94-95 22.20668726999464 26.181302845794423 28.095263605793168 23.516746278417767 96-97 22.331573133675537 26.204734534039915 27.954560558548426 23.50913177373613 98-99 22.15836546746414 26.206080223537025 27.898534707057053 23.73701960194178 100-101 22.426104354775205 25.772020133864732 28.170871467316132 23.631004044043927 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2168.0 1 1245.5 2 302.0 3 251.0 4 192.5 5 186.5 6 264.0 7 442.0 8 729.5 9 983.0 10 1027.0 11 1029.5 12 1144.5 13 1281.0 14 1340.5 15 1401.0 16 1552.5 17 1699.0 18 1844.5 19 1951.0 20 2129.0 21 2455.0 22 2870.5 23 3508.0 24 4370.5 25 5592.0 26 7426.5 27 9493.5 28 11505.0 29 13928.0 30 16832.0 31 19860.5 32 23295.0 33 26564.5 34 30469.0 35 34366.5 36 38383.5 37 42441.5 38 45654.5 39 48008.5 40 49508.5 41 52451.0 42 55315.5 43 58418.5 44 62625.0 45 65106.0 46 67061.5 47 69375.0 48 70338.5 49 69493.5 50 67380.5 51 64720.5 52 62972.0 53 62465.0 54 61804.0 55 57805.5 56 51612.5 57 46430.0 58 42136.0 59 36944.0 60 30263.5 61 24514.5 62 19349.5 63 14531.5 64 10706.0 65 7861.0 66 5712.5 67 4322.0 68 3239.0 69 2297.5 70 1653.5 71 1147.0 72 802.0 73 579.5 74 405.0 75 254.5 76 163.0 77 95.5 78 68.5 79 57.5 80 30.0 81 14.0 82 15.5 83 12.0 84 9.5 85 7.0 86 7.0 87 10.0 88 9.0 89 6.5 90 4.0 91 2.0 92 0.5 93 1.0 94 1.5 95 1.5 96 2.0 97 1.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21890750777951465 2 0.19857119899487208 3 0.19243524375812648 4 0.19786994696781546 5 0.19675963125830911 6 0.21469999561717482 7 0.21464155794825346 8 0.20657715963710208 9 0.20120089409633451 10-11 0.21285920904615113 12-13 0.21420327543134307 14-15 0.20383058919779692 16-17 0.21282999021169044 18-19 0.20409355870794313 20-21 0.19871729316717554 22-23 0.19798682230565823 24-25 0.19310727695072244 26-27 0.20006135955236748 28-29 0.20748294350538357 30-31 0.20754138117430493 32-33 0.20654794080264138 34-35 0.21464155794825346 36-37 0.19792838463673681 38-39 0.19854198016041139 40-41 0.19567853438326346 42-43 0.19839588598810792 44-45 0.2104632646203743 46-47 0.21370655524551124 48-49 0.21639468801589504 50-51 0.21694984587064822 52-53 0.22960160119212844 54-55 0.21639468801589504 56-57 0.19781150929889407 58-59 0.1862116320179988 60-61 0.18425397010913236 62-63 0.19041914418033865 64-65 0.18457537728819998 66-67 0.1844585019503572 68-69 0.189338047305293 70-71 0.18939648497421438 72-73 0.19036070651141726 74-75 0.19097430203509183 76-77 0.1989510438428611 78-79 0.19626291107247731 80-81 0.1915002410553843 82-83 0.19047758184926003 84-85 0.19155867872430568 86-87 0.19570775321772413 88-89 0.1910911773729346 90-91 0.19792838463673681 92-93 0.19097430203509183 94-95 0.1898347674911248 96-97 0.19690572543061258 98-99 0.19924323218746803 100-101 0.1845169396192786 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1711225.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.59639262707316 #Duplication Level Percentage of deduplicated Percentage of total 1 77.43286791548881 44.5985386270079 2 12.696497056679501 14.625448589299827 3 4.035695646036483 6.973245328574608 4 1.8629072297755376 4.291869449358603 5 0.9954556265476853 2.8667326554734793 6 0.6500285863159296 2.246358100576416 7 0.4110597745944987 1.6572892119518658 8 0.30211790406850175 1.3920721137918282 9 0.2255918908861855 1.1693951208868127 >10 1.2773535134260658 13.421164140433694 >50 0.07912034372056834 3.100882176643353 >100 0.03059546919245324 2.8281029777630673 >500 3.0387568620940896E-4 0.11469737930721233 >1k 4.0516758161254535E-4 0.7142041289312783 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4462 0.2607488787272276 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3435 0.2007333927449634 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2302 0.13452351385703226 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2043 0.11938815760639308 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 5.843766892138672E-5 14-15 0.0 0.0 0.0 0.0 5.843766892138672E-5 16-17 0.0 0.0 0.0 0.0 5.843766892138672E-5 18-19 0.0 0.0 0.0 0.0 5.843766892138672E-5 20-21 0.0 0.0 0.0 1.1687533784277346E-4 5.843766892138672E-5 22-23 0.0 0.0 0.0 3.506260135283203E-4 5.843766892138672E-5 24-25 0.0 0.0 0.0 5.551578547531739E-4 5.843766892138672E-5 26-27 0.0 0.0 0.0 0.0010226592061242678 5.843766892138672E-5 28-29 0.0 0.0 0.0 0.0021037560811699224 5.843766892138672E-5 30-31 0.0 0.0 0.0 0.0046457946792502446 5.843766892138672E-5 32-33 0.0 0.0 0.0 0.007947522973308595 5.843766892138672E-5 34-35 0.0 0.0 0.0 0.011804409122120118 5.843766892138672E-5 36-37 0.0 0.0 0.0 0.01779427018656226 5.843766892138672E-5 38-39 0.0 0.0 0.0 0.026530701690309574 5.843766892138672E-5 40-41 0.0 0.0 0.0 0.03792604712997999 5.843766892138672E-5 42-43 0.0 0.0 0.0 0.05338281055968677 5.843766892138672E-5 44-45 0.0 0.0 0.0 0.06714488159067335 5.843766892138672E-5 46-47 0.0 0.0 0.0 0.0951073061695569 5.843766892138672E-5 48-49 0.0 0.0 0.0 0.12976084383993924 5.843766892138672E-5 50-51 0.0 0.0 0.0 0.14857777323262578 5.843766892138672E-5 52-53 0.0 0.0 0.0 0.18028020862247807 5.843766892138672E-5 54-55 0.0 0.0 0.0 0.2315884819354556 5.843766892138672E-5 56-57 0.0 0.0 0.0 0.3101871466347207 5.843766892138672E-5 58-59 0.0 0.0 0.0 0.41435229148709257 5.843766892138672E-5 60-61 0.0 0.0 0.0 0.5245072974039066 5.843766892138672E-5 62-63 0.0 0.0 0.0 0.6362109015471373 5.843766892138672E-5 64-65 0.0 0.0 0.0 0.7624362664173326 5.843766892138672E-5 66-67 0.0 0.0 0.0 0.9269090855965756 5.843766892138672E-5 68-69 0.0 0.0 0.0 1.1178541687972068 5.843766892138672E-5 70-71 0.0 0.0 0.0 1.2478487633128315 5.843766892138672E-5 72-73 0.0 0.0 0.0 1.4218177036917998 5.843766892138672E-5 74-75 0.0 0.0 0.0 1.6188987421291765 1.1687533784277345E-4 76-77 0.0 0.0 0.0 1.8397054741486363 1.1687533784277345E-4 78-79 0.0 0.0 0.0 2.0789785095472544 1.1687533784277345E-4 80-81 0.0 0.0 0.0 2.4133588511154294 1.1687533784277345E-4 82-83 0.0 0.0 0.0 2.788528685590732 1.1687533784277345E-4 84-85 0.0 0.0 0.0 3.2625166182120995 1.1687533784277345E-4 86-87 0.0 0.0 0.0 3.827784189688673 1.1687533784277345E-4 88-89 0.0 0.0 0.0 4.491022513111952 1.1687533784277345E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2980 0.0 44.4836 1 GTATCAA 4895 0.0 37.95212 1 ATCAACG 6270 0.0 29.47255 3 TCAACGC 6340 0.0 29.299576 4 CAACGCA 6505 0.0 28.701616 5 TATCAAC 6445 0.0 28.672285 2 AACGCAG 6700 0.0 28.005621 6 GTACATG 5445 0.0 25.654375 1 ACGCAGA 7630 0.0 24.093319 7 TACATGG 5705 0.0 24.064554 2 CGCAGAG 7585 0.0 23.98646 8 ACATGGG 5460 0.0 23.57829 3 GCAGAGT 8210 0.0 21.29317 9 GAGTACT 4500 0.0 20.900494 12-13 AGAGTAC 7285 0.0 20.181232 10-11 GTATAGG 415 0.0 19.463135 1 GTACTTT 4755 0.0 19.279877 14-15 AGTACTT 4690 0.0 19.142242 12-13 CATGGGG 3465 0.0 19.058353 4 CAGAGTA 7935 0.0 18.46821 10-11 >>END_MODULE