FastQCFastQC Report
Thu 26 May 2016
SRR937384_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937384_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1711225
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48030.2806761238294204No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39830.23275723531388331No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25170.1470876126751304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34400.043.3884541
GTATCAA54650.038.096661
ATCAACG66650.030.9991173
TCAACGC67350.030.676934
TATCAAC68600.030.1871832
CAACGCA68650.030.096015
AACGCAG71150.029.0385256
ACGCAGA81350.025.2852177
CGCAGTA950.001227349924.9980772
GTACATG58200.024.9103491
CGCAGAG82150.024.8076788
TACATGG59450.024.0477712
ACATGGG58200.023.4217383
GCAGAGT91300.021.9052249
GGACCGA3500.021.7126166
GTACTTT49600.021.16268714-15
GAGTACT50200.020.72415212-13
GTGTAGG6200.019.9335481
AGAGTAC78100.019.7682710-11
CATGGGG36600.019.4657154
Produced by FastQC (version 0.11.5)