##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937384_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1711225 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.25448085435872 33.0 31.0 34.0 31.0 34.0 2 32.47036129088811 34.0 31.0 34.0 31.0 34.0 3 32.530498911598414 34.0 31.0 34.0 31.0 34.0 4 36.150922292509755 37.0 35.0 37.0 35.0 37.0 5 36.063483761632746 37.0 35.0 37.0 35.0 37.0 6 36.09936273722041 37.0 35.0 37.0 35.0 37.0 7 36.0391006442753 37.0 35.0 37.0 35.0 37.0 8 36.015010299639144 37.0 35.0 37.0 35.0 37.0 9 37.73580505193648 39.0 37.0 39.0 35.0 39.0 10-11 37.65570716884103 39.0 37.0 39.0 35.0 39.0 12-13 37.558364037458546 39.0 37.0 39.0 35.0 39.0 14-15 38.80552937223334 40.0 38.0 41.0 35.0 41.0 16-17 38.76691200748002 40.0 38.0 41.0 35.0 41.0 18-19 38.73199725342956 40.0 38.0 41.0 34.5 41.0 20-21 38.61502929188154 40.0 38.0 41.0 34.0 41.0 22-23 38.45961168169002 40.0 38.0 41.0 34.0 41.0 24-25 38.40583558561849 40.0 38.0 41.0 34.0 41.0 26-27 38.362548758930004 40.0 38.0 41.0 34.0 41.0 28-29 38.23237943578431 40.0 38.0 41.0 34.0 41.0 30-31 38.3672760741574 40.0 38.0 41.0 34.0 41.0 32-33 38.348222472205585 40.0 38.0 41.0 34.0 41.0 34-35 38.24579058861342 40.0 38.0 41.0 34.0 41.0 36-37 38.11289456383585 40.0 38.0 41.0 33.5 41.0 38-39 37.999850983944256 40.0 38.0 41.0 33.0 41.0 40-41 37.843876170579556 40.0 37.0 41.0 33.0 41.0 42-43 37.661658168855645 40.0 37.0 41.0 33.0 41.0 44-45 37.42876857222166 40.0 37.0 41.0 32.5 41.0 46-47 37.146235007085565 40.0 37.0 41.0 31.5 41.0 48-49 36.879720083565864 40.0 36.0 41.0 31.0 41.0 50-51 36.6614410729156 39.0 36.0 41.0 31.0 41.0 52-53 36.42339639731771 39.0 36.0 41.0 30.0 41.0 54-55 36.19990883723648 39.0 35.0 40.0 30.0 41.0 56-57 35.923896331575335 39.0 35.0 40.0 29.0 41.0 58-59 35.64522987918012 38.0 35.0 40.0 28.5 41.0 60-61 35.31517275635875 38.0 34.0 40.0 28.0 41.0 62-63 34.9442177387544 37.5 34.0 40.0 28.0 41.0 64-65 34.539376177884265 37.0 34.0 40.0 27.0 41.0 66-67 34.119563762801505 36.5 33.5 39.0 26.0 40.5 68-69 33.67346287016611 36.0 33.0 39.0 26.0 40.0 70-71 33.26219813291648 35.0 32.5 38.5 26.0 40.0 72-73 32.79149585823022 35.0 32.0 37.5 25.0 39.5 74-75 32.316642171543776 35.0 32.0 37.0 24.0 39.0 76-77 31.056597758915395 34.0 30.5 35.5 22.5 38.0 78-79 31.66800654501892 34.0 31.0 36.0 24.0 38.5 80-81 31.480139958217066 34.0 31.0 36.0 23.0 37.0 82-83 31.156990460050547 34.0 31.0 35.0 22.0 37.0 84-85 30.81197942994054 34.0 31.0 35.0 20.5 36.5 86-87 30.42041549182603 34.0 31.0 35.0 19.5 36.0 88-89 29.981240631711202 34.0 30.0 35.0 17.5 36.0 90-91 29.50161112653216 34.0 29.5 35.0 10.0 35.0 92-93 29.14880889421321 34.0 29.0 35.0 4.5 35.0 94-95 28.738282809098745 34.0 29.0 35.0 2.0 35.0 96-97 28.27847536121784 34.0 29.0 35.0 2.0 35.0 98-99 27.53803941620769 33.0 28.0 35.0 2.0 35.0 100-101 25.069017224502915 31.0 21.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.00661679019317063 1101 2 -0.07201786344238315 1101 3 -0.03220958122248874 1101 4 -0.040873474773583496 1101 5 -0.062202870986162395 1101 6 -0.10205398990238734 1101 7 -0.0390688909852841 1101 8 -0.058274255142841014 1101 9 -0.07554007299290078 1101 10-11 -0.025527615148604355 1101 12-13 -0.05201906180851523 1101 14-15 -0.13307051654671653 1101 16-17 -0.17262767759668662 1101 18-19 -0.12706450542133751 1101 20-21 -0.15317664342717308 1101 22-23 -0.16959120507985403 1101 24-25 -0.11790861184625356 1101 26-27 -0.1717202565972471 1101 28-29 -0.2530028554822721 1101 30-31 -0.208297131060192 1101 32-33 -0.152917629303424 1101 34-35 -0.11497216851106629 1101 36-37 -0.17841990685534626 1101 38-39 -0.19373116209651187 1101 40-41 -0.0721209084933534 1101 42-43 -0.12913448989228726 1101 44-45 -0.26428765876027427 1101 46-47 -0.3947492324509838 1101 48-49 -0.3577476926835246 1101 50-51 -0.23046878414678673 1101 52-53 -0.2576954723608438 1101 54-55 -0.3445508674827167 1101 56-57 -0.31579901494745144 1101 58-59 -0.1982433749942345 1101 60-61 -0.3528972003821522 1101 62-63 -0.3920774932428799 1101 64-65 -0.41740189619445545 1101 66-67 -0.3956510870002319 1101 68-69 -0.4905450590692695 1101 70-71 -0.46098489920852614 1101 72-73 -0.4835349735463197 1101 74-75 -0.5499840674415317 1101 76-77 -0.42765478122326783 1101 78-79 -0.5901363311162662 1101 80-81 -0.6402924227502069 1101 82-83 -0.6931766364319216 1101 84-85 -0.635246139424428 1101 86-87 -0.6207726760075012 1101 88-89 -0.6644028279431211 1101 90-91 -0.7521586979776984 1101 92-93 -0.7421816998365465 1101 94-95 -0.7862413190915198 1101 96-97 -0.7607552880287898 1101 98-99 -0.7227805819204214 1101 100-101 -0.15860324194384035 1102 1 -0.011661034277793192 1102 2 -0.05267401890541379 1102 3 -0.014572106119857153 1102 4 -0.012690037653044328 1102 5 -0.08884530838329852 1102 6 -0.14767504139974363 1102 7 -0.14678740526743894 1102 8 -0.09542321715714053 1102 9 -0.0910079563100652 1102 10-11 -0.08198332380594309 1102 12-13 -0.07783431643576222 1102 14-15 -0.012562319516391085 1102 16-17 -0.060699454735534175 1102 18-19 -0.14951808927173005 1102 20-21 -0.08888378743878889 1102 22-23 -0.08951028755468826 1102 24-25 -0.14532248490782962 1102 26-27 -0.1870075299876035 1102 28-29 -0.11744267260502994 1102 30-31 -0.11626648057970357 1102 32-33 -0.06135260752395766 1102 34-35 -0.07812319811465329 1102 36-37 -0.10962450287666314 1102 38-39 -0.16601435632276917 1102 40-41 -0.1505044803962292 1102 42-43 0.03269768618728364 1102 44-45 -0.042971583233509136 1102 46-47 0.024719929440074395 1102 48-49 0.18747243418725645 1102 50-51 0.037517377737195545 1102 52-53 0.07956021127045432 1102 54-55 0.035596799514287625 1102 56-57 0.0051690221755720245 1102 58-59 0.09466314673530718 1102 60-61 -0.05716615864253782 1102 62-63 0.01618289092552061 1102 64-65 -0.08694007353682309 1102 66-67 -0.013420189441092134 1102 68-69 0.04932882680745365 1102 70-71 0.07198409938496297 1102 72-73 -0.001577718073917822 1102 74-75 0.018056143734384023 1102 76-77 -0.011235133055677693 1102 78-79 -0.011987044111052114 1102 80-81 -0.19549408890685882 1102 82-83 -0.07536054332543429 1102 84-85 -0.1672223181648853 1102 86-87 -0.17672378646443931 1102 88-89 0.03391188492118147 1102 90-91 0.0298904399541442 1102 92-93 0.06982522403416525 1102 94-95 0.16178680418684976 1102 96-97 0.1886377035306701 1102 98-99 0.3300851869371044 1102 100-101 0.6325233161509907 1103 1 0.05880128135852658 1103 2 0.104321203167963 1103 3 0.04875883395224889 1103 4 0.0054774842991349715 1103 5 0.06129089034877211 1103 6 0.06370285726261216 1103 7 0.04171969285581412 1103 8 -0.06639010826555847 1103 9 0.04918240975032262 1103 10-11 0.012007373691716339 1103 12-13 -0.0011891076859242844 1103 14-15 -0.04619035424175166 1103 16-17 -0.2116680921555485 1103 18-19 -0.06954108991461538 1103 20-21 -0.04487537799838748 1103 22-23 0.04809227997576926 1103 24-25 -0.003604322407653626 1103 26-27 -0.018463804562074415 1103 28-29 -0.08105922058132364 1103 30-31 -0.1366999096887298 1103 32-33 0.004045178279618256 1103 34-35 -0.016463921603403264 1103 36-37 -0.024015196212126 1103 38-39 -0.07658239167245995 1103 40-41 -0.0031562726502940563 1103 42-43 0.014758406255801049 1103 44-45 -0.08400402436448218 1103 46-47 0.09424959705726366 1103 48-49 0.029904231897418754 1103 50-51 0.050852457382127625 1103 52-53 0.15295981396194946 1103 54-55 -0.040729232336445875 1103 56-57 -0.07182703233648624 1103 58-59 -0.051816203130080396 1103 60-61 -0.017202767848317535 1103 62-63 0.06311614608200955 1103 64-65 -0.15536291438766625 1103 66-67 0.03235217567556958 1103 68-69 -0.0410674547251233 1103 70-71 0.06071857994547969 1103 72-73 0.09747049686536968 1103 74-75 0.14362069777119757 1103 76-77 0.3364014423096542 1103 78-79 0.2691275008096454 1103 80-81 0.10481201327589318 1103 82-83 0.15366007898582268 1103 84-85 0.12836670685487306 1103 86-87 0.10576224643588716 1103 88-89 0.24286485349255216 1103 90-91 0.2527926379089678 1103 92-93 0.2823970388364607 1103 94-95 0.3705018038724255 1103 96-97 0.3970258353075238 1103 98-99 0.6472217064295158 1103 100-101 1.0009563815205027 1104 1 0.07293252070146394 1104 2 0.0753733391852407 1104 3 0.044274534412927835 1104 4 0.05892997742466122 1104 5 0.027199114353784637 1104 6 0.09312236392265305 1104 7 0.07768498132931967 1104 8 0.06020428453014404 1104 9 0.17918414168408958 1104 10-11 0.047175422812657075 1104 12-13 0.06362207029706468 1104 14-15 0.031393491627135006 1104 16-17 -0.008752292314859744 1104 18-19 0.05612599667725959 1104 20-21 0.05376546636055224 1104 22-23 -0.05221984610351171 1104 24-25 -0.04501875903778085 1104 26-27 -0.18398667330417595 1104 28-29 0.07029246094079866 1104 30-31 -0.028442107911288872 1104 32-33 -0.020169420684929662 1104 34-35 0.010553471959987348 1104 36-37 0.12374263683632591 1104 38-39 0.17736179538738384 1104 40-41 0.08807986758410635 1104 42-43 0.12414265225637422 1104 44-45 0.08607075462496283 1104 46-47 0.09413318980266183 1104 48-49 0.21475946766377518 1104 50-51 0.010166103027096085 1104 52-53 0.18630074168738275 1104 54-55 0.13670961562520034 1104 56-57 0.06668926742825221 1104 58-59 0.12389655290574098 1104 60-61 0.16611305714664582 1104 62-63 0.17787686087581278 1104 64-65 0.2967400105872997 1104 66-67 0.2880401520003062 1104 68-69 0.19538643494382057 1104 70-71 0.2246545300108096 1104 72-73 0.22853544369431944 1104 74-75 0.19765428216619796 1104 76-77 0.2962685163931198 1104 78-79 0.09655026652917797 1104 80-81 0.11706707694851559 1104 82-83 0.13537279997060736 1104 84-85 0.11730483901928679 1104 86-87 0.17200914252879684 1104 88-89 0.3333266062782485 1104 90-91 0.26439410276755027 1104 92-93 0.6015715559052843 1104 94-95 0.5899165064780938 1104 96-97 0.5763557212679338 1104 98-99 0.7625363651182901 1104 100-101 1.3512306215486518 1105 1 0.0450698187530989 1105 2 0.04975564108351449 1105 3 -2.457096247852064E-4 1105 4 0.04810822872625664 1105 5 -0.027509525368543564 1105 6 -0.05785181889093849 1105 7 -0.029141454897043673 1105 8 -0.04349421080916471 1105 9 -0.053023290373587884 1105 10-11 -0.07651323229620743 1105 12-13 -0.0691996605100087 1105 14-15 -0.044909891622999965 1105 16-17 -0.04870237550954215 1105 18-19 -0.11211027620127112 1105 20-21 -0.20327566580268552 1105 22-23 -0.147048357723925 1105 24-25 -0.1565022185049756 1105 26-27 -0.011076827706339998 1105 28-29 -0.06905298482163857 1105 30-31 -0.13820102226550546 1105 32-33 -0.09767125454606429 1105 34-35 0.011839232251979581 1105 36-37 -0.04695906105070691 1105 38-39 -0.14679731219316494 1105 40-41 -0.09393827422255896 1105 42-43 0.004312709329710174 1105 44-45 0.0960348825121713 1105 46-47 -0.19550030634176352 1105 48-49 -0.01894761551300661 1105 50-51 0.02110521883766836 1105 52-53 -0.04273777834195158 1105 54-55 0.12119479265989241 1105 56-57 0.07305204988760039 1105 58-59 0.17713237850107788 1105 60-61 0.0359752044638455 1105 62-63 0.05150164394346035 1105 64-65 0.21153452868437483 1105 66-67 0.232749239005841 1105 68-69 0.2742336072194149 1105 70-71 0.29682975767795483 1105 72-73 0.30942983118470124 1105 74-75 0.3004982876767315 1105 76-77 0.4075706472502958 1105 78-79 0.2712258072945666 1105 80-81 0.2727252989083304 1105 82-83 0.253624369934645 1105 84-85 0.21301181484810527 1105 86-87 0.1968368490394532 1105 88-89 0.30862387069418773 1105 90-91 0.35476436380202614 1105 92-93 0.5808125163117772 1105 94-95 0.7409357754384267 1105 96-97 0.5243368638081414 1105 98-99 0.7261155252993525 1105 100-101 1.2220617631942972 1106 1 -0.27416151679387823 1106 2 -0.09565255539970252 1106 3 -0.13587061259016053 1106 4 -0.0903349102373383 1106 5 -0.20376065848251557 1106 6 -0.08724617260279643 1106 7 -0.13315655974452056 1106 8 -0.16920881132701737 1106 9 -0.2600967261754832 1106 10-11 -0.261601402335927 1106 12-13 -0.25756972927312205 1106 14-15 -0.2948089756161707 1106 16-17 -0.15758919432649066 1106 18-19 -0.06999569771966918 1106 20-21 -0.15954072181983037 1106 22-23 -0.14720611723833343 1106 24-25 -0.20043228346182929 1106 26-27 -0.21403769833852238 1106 28-29 -0.29625012411577956 1106 30-31 -0.11619301910030089 1106 32-33 -0.12341199806915881 1106 34-35 -0.05248548190281355 1106 36-37 -0.11597679522342474 1106 38-39 -0.18809174152942632 1106 40-41 -0.1261218161490305 1106 42-43 -0.4157474896976865 1106 44-45 -0.0840293231060727 1106 46-47 -0.2667103468584102 1106 48-49 -0.3014499318233419 1106 50-51 -0.025312338043399052 1106 52-53 0.04562908514848374 1106 54-55 -0.15027307197743767 1106 56-57 -0.19241574463439548 1106 58-59 -0.247745145025128 1106 60-61 -0.06483693149981917 1106 62-63 -0.08580783014140536 1106 64-65 -0.0864051821399272 1106 66-67 -0.0740028682105276 1106 68-69 0.01054303623013908 1106 70-71 -0.04158683762130977 1106 72-73 -0.033561689867426026 1106 74-75 0.017348906945422016 1106 76-77 0.05634177575668531 1106 78-79 0.004313837103605778 1106 80-81 0.04783405280849706 1106 82-83 -0.22530256487311462 1106 84-85 -0.06466346098063269 1106 86-87 -0.01608861334617373 1106 88-89 0.1674873010371023 1106 90-91 0.10420879989941056 1106 92-93 0.2611492702720426 1106 94-95 0.15326535760108229 1106 96-97 0.46943545098404726 1106 98-99 0.6210238494926479 1106 100-101 1.2876557653278056 1107 1 -0.07387654505679109 1107 2 -0.04335054966535523 1107 3 -0.007385749072518877 1107 4 -0.049360117650273594 1107 5 -0.09934129988021567 1107 6 -0.06598950419984106 1107 7 -0.15612815719391904 1107 8 -0.04144992754900301 1107 9 -0.10828956256293765 1107 10-11 -0.047721478144651996 1107 12-13 -0.048677614317988116 1107 14-15 -0.030511469532271462 1107 16-17 0.017017545595003014 1107 18-19 -0.029230591783125703 1107 20-21 -0.16655110004585794 1107 22-23 -0.06771546976820986 1107 24-25 -0.03985273068923334 1107 26-27 -0.13178529624358504 1107 28-29 -0.0924343553121929 1107 30-31 0.01211201457358868 1107 32-33 0.09618826062858687 1107 34-35 0.031026418101689046 1107 36-37 0.08472605697436109 1107 38-39 0.006485757094864653 1107 40-41 -0.041709159251503536 1107 42-43 -0.12162131115766073 1107 44-45 0.10680665829635672 1107 46-47 0.24639954731988212 1107 48-49 0.3224715484495775 1107 50-51 0.35548964334616784 1107 52-53 0.25928232727616773 1107 54-55 0.3109721038515971 1107 56-57 0.4254847660548009 1107 58-59 0.3679248325317417 1107 60-61 0.45817600614355314 1107 62-63 0.4011109841483318 1107 64-65 0.274144123390748 1107 66-67 0.2157214983428588 1107 68-69 0.29761784085179244 1107 70-71 0.29497126050928557 1107 72-73 0.498473859943374 1107 74-75 0.4977487171479993 1107 76-77 0.3740518459523372 1107 78-79 0.22120608342869374 1107 80-81 0.4923349572400717 1107 82-83 0.44933506535997836 1107 84-85 0.38103688324538254 1107 86-87 0.3805102599957415 1107 88-89 0.39151119015718905 1107 90-91 0.35423245567706374 1107 92-93 0.6615636138494736 1107 94-95 0.4878114514580254 1107 96-97 0.6863388361947322 1107 98-99 1.0756084310701723 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20-21 0.4211200007831337 2316 22-23 0.5601430077866212 2316 24-25 0.36891697463918405 2316 26-27 0.4909160577330667 2316 28-29 0.5640200202717409 2316 30-31 0.5116930677983618 2316 32-33 0.5025777550747605 2316 34-35 0.2624722664503736 2316 36-37 0.3453313423772144 2316 38-39 0.019024500402835542 2316 40-41 -0.15070761693417722 2316 42-43 -0.07663643773149431 2316 44-45 -0.03207975893014492 2316 46-47 -0.009258087892412448 2316 48-49 -0.23452931168893087 2316 50-51 0.3375527893769217 2316 52-53 0.656647782333927 2316 54-55 0.36113819956425175 2316 56-57 0.5342793115902467 2316 58-59 0.43525106230736554 2316 60-61 0.6311009219834531 2316 62-63 0.6464098822812403 2316 64-65 0.5886563655345682 2316 66-67 0.44903592345890786 2316 68-69 0.6171036856412826 2316 70-71 0.6435053175453618 2316 72-73 0.5143623237063295 2316 74-75 0.8402080459433563 2316 76-77 0.7474057188001382 2316 78-79 0.5130303185727634 2316 80-81 0.3434957734818589 2316 82-83 0.4739954589475026 2316 84-85 0.5987705665661096 2316 86-87 0.2643192647561605 2316 88-89 0.4436533799512006 2316 90-91 0.7058622698574908 2316 92-93 0.8866399882173468 2316 94-95 1.2333272379657139 2316 96-97 1.0263920016527557 2316 98-99 0.8987535720290758 2316 100-101 0.3349310331433628 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 4.0 4 10.0 5 31.0 6 71.0 7 179.0 8 381.0 9 744.0 10 1219.0 11 1906.0 12 2808.0 13 3778.0 14 4920.0 15 6683.0 16 8962.0 17 12378.0 18 11836.0 19 7713.0 20 7252.0 21 7531.0 22 7853.0 23 8821.0 24 10417.0 25 12233.0 26 15039.0 27 18798.0 28 23999.0 29 29917.0 30 37861.0 31 47894.0 32 61396.0 33 81901.0 34 112925.0 35 164122.0 36 258137.0 37 398208.0 38 315316.0 39 27978.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.57992870275505 19.502533297778278 14.867452152718325 23.05008584674835 2 15.849406127189585 20.56047568262502 38.92761033762363 24.66250785256176 3 19.46815877514646 23.98270244999927 29.626787827433564 26.92235094742071 4 13.474499262224429 16.838755862028666 36.24999634764569 33.43674852810121 5 14.110122195665006 37.190610043303195 34.11731017595942 14.581957585072377 6 33.103420064573626 35.96382708293766 16.789785095472542 14.142967757016173 7 29.15840991102865 32.34180192552119 20.290961153559582 18.208827009890573 8 26.868354541337347 35.22073368493331 19.19770924337828 18.713202530351065 9 26.32061827053719 16.70832298499613 19.855951146108783 37.1151075983579 10-11 25.129132789823416 26.72930412926895 26.997562561980526 21.144000518927104 12-13 25.850794538528316 23.769709327145684 27.70112856817679 22.67836756614921 14-15 22.92065625502199 25.36951599000716 25.58646583587781 26.123361919093046 16-17 22.54706423760756 28.697249046735525 26.189601016815438 22.566085698841473 18-19 22.610498327221727 27.37419100352087 28.218060161580155 21.797250507677248 20-21 23.105523820654795 26.45715788397201 28.516092273079224 21.921226022293972 22-23 22.940114439791127 26.24018564690214 28.427269547696056 22.39243036561068 24-25 22.84592191447765 26.467211718467627 28.45714459358812 22.229721773466608 26-27 22.668700694855744 26.585354237346078 28.380186399340584 22.365758668457595 28-29 22.576993620426325 26.90544947607317 28.31162638074832 22.205930522752197 30-31 22.71261944325702 26.692381256535906 28.302986085077876 22.292013215129202 32-33 22.73158117722684 26.595100001460942 28.256307615889202 22.417011205423016 34-35 22.900349165071805 26.68196759631258 28.20190214612339 22.21578109249222 36-37 23.00583587951201 26.502980760268454 28.13537908088623 22.35580427933331 38-39 22.77375611224172 26.68290267017489 28.242961517165412 22.300379700417977 40-41 22.916338563408832 26.76241486724857 28.074340917863204 22.24690565147939 42-43 22.742092293180185 26.698436883140715 27.920473934951122 22.638996888727974 44-45 22.866641499466464 26.792724731215472 27.906256464678457 22.434377304639607 46-47 23.013718242779298 27.054624611024263 27.432599453607796 22.499057692588643 48-49 23.01617262487399 27.15335505266695 27.32787915090067 22.502593171558384 50-51 23.118906046837793 27.18710280646905 27.280427763736505 22.413563382956653 52-53 23.12630516919317 27.279646826000914 27.02065100166577 22.57339700314015 54-55 23.05985478239273 27.26517553214802 27.05225788543295 22.622711800026295 56-57 23.043784423439348 27.4740025420386 27.071922161024997 22.410290873497058 58-59 23.007319318032405 27.197300179695834 27.15478677555552 22.64059372671624 60-61 23.01015354497509 27.205072389662377 27.32820055807974 22.456573507282794 62-63 22.802173881283874 27.23440809946091 27.478502242545545 22.484915776709666 64-65 22.875688468786983 27.185232801063563 27.351254218469222 22.58782451168023 66-67 22.797878712618154 27.340998407573526 27.155604902920423 22.7055179768879 68-69 22.75314467705883 27.17819106195854 27.353854694736228 22.7148095662464 70-71 22.858566231793016 27.099504740755894 27.30973425470058 22.732194772750518 72-73 22.725269908983332 27.1294540460781 27.15762100249821 22.98765504244036 74-75 22.71983520577364 27.50219871729317 26.825987231369343 22.95197884556385 76-77 22.892752268112023 27.279989481219598 27.05696211778112 22.77029613288726 78-79 22.875104092097768 27.223567911875996 26.968487487034142 22.932840508992097 80-81 22.86751387675901 27.259982544663192 26.883906816434667 22.988596762143132 82-83 22.745533229372388 27.33430749240527 26.96945073381878 22.950708544403568 84-85 22.69599118990357 27.26069172347073 26.914155030465132 23.129162056160567 86-87 22.700726088036348 27.13158702099373 27.007348536866864 23.160338354103054 88-89 22.71381612587474 27.079402182646934 26.915250770646757 23.29153092083157 90-91 22.694979505831494 27.058789495507707 27.026324932579783 23.21990606608102 92-93 22.46189107461194 26.945725480158288 27.12487081286866 23.46751263236111 94-95 22.264401233034814 27.228593551403236 27.148533944980933 23.358471270581017 96-97 22.38224079241479 27.22242837733203 26.946339610512936 23.448991219740243 98-99 22.11763812128464 27.307524299148707 26.713037024700174 23.861800554866477 100-101 22.034969602132016 27.16176984116548 26.949328120182987 23.85393243651952 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 139.0 1 98.0 2 76.0 3 103.5 4 109.5 5 114.5 6 201.0 7 426.0 8 709.0 9 905.0 10 971.0 11 1048.0 12 1209.5 13 1359.5 14 1456.5 15 1535.0 16 1625.5 17 1711.0 18 1850.0 19 2003.5 20 2179.0 21 2497.5 22 2959.0 23 3644.5 24 4434.0 25 5600.0 26 7447.5 27 9516.5 28 11662.5 29 14170.0 30 16918.0 31 19762.5 32 23194.5 33 26225.5 34 30110.0 35 34189.0 36 38170.0 37 42476.0 38 45915.5 39 48182.5 40 49938.5 41 52573.5 42 55184.5 43 58780.0 44 63133.0 45 65603.5 46 66670.0 47 69153.5 48 71189.0 49 69759.5 50 67002.5 51 64719.0 52 62923.0 53 62224.5 54 62025.0 55 58472.0 56 51637.0 57 46036.0 58 42113.5 59 37060.0 60 30485.0 61 24503.5 62 19312.5 63 14678.0 64 10650.0 65 7566.0 66 5643.0 67 4435.0 68 3263.0 69 2426.0 70 1791.0 71 1123.5 72 753.0 73 551.5 74 398.5 75 225.5 76 135.5 77 89.5 78 59.0 79 40.0 80 24.0 81 14.0 82 8.0 83 4.5 84 3.0 85 2.5 86 1.0 87 3.0 88 2.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07018364037458547 2 0.0 3 0.0 4 0.0 5 0.0020453184122485356 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.1687533784277345E-4 12-13 0.004353606334643311 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0011395345439670413 24-25 6.720331925959474E-4 26-27 3.7984484798901376E-4 28-29 0.003535478969743897 30-31 2.629695101462403E-4 32-33 0.0 34-35 0.0 36-37 2.921883446069336E-5 38-39 4.3828251691040046E-4 40-41 2.921883446069336E-5 42-43 0.011015500591681398 44-45 1.7531300676416016E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.921883446069336E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.921883446069336E-5 82-83 3.21407179067627E-4 84-85 3.7984484798901376E-4 86-87 0.0 88-89 0.0 90-91 0.007509240456398194 92-93 0.0026004762670017094 94-95 0.0 96-97 0.0 98-99 1.460941723034668E-4 100-101 0.009788309544332277 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1711225.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.25916121333674 #Duplication Level Percentage of deduplicated Percentage of total 1 75.83213699830851 41.90420283541367 2 13.340418708599547 14.743606961438315 3 4.452049357233913 7.3804953948336305 4 2.0314344280150918 4.490214502080338 5 1.1110665458449567 3.069830268779582 6 0.6868344184544887 2.277233631374698 7 0.4556553777254705 1.762539378281893 8 0.3352727464897653 1.4821512598972895 9 0.24134428623682355 1.2002834538970486 >10 1.3879921522270975 14.133899886374135 >50 0.08779968394542016 3.3116434185487718 >100 0.03704289525147289 3.3207878000111832 >500 5.291120374448921E-4 0.20232894864942375 >1k 4.2328962995591375E-4 0.7207822604198836 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4803 0.2806761238294204 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3983 0.23275723531388331 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2517 0.1470876126751304 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 8.76565033820801E-5 0.0 18-19 0.0 0.0 0.0 2.337506756855469E-4 0.0 20-21 0.0 0.0 0.0 3.506260135283203E-4 0.0 22-23 2.921883446069336E-5 0.0 0.0 5.843766892138672E-4 0.0 24-25 5.843766892138672E-5 0.0 0.0 7.596896959780274E-4 0.0 26-27 5.843766892138672E-5 0.0 0.0 0.001285628716270508 0.0 28-29 5.843766892138672E-5 0.0 0.0 0.0024543820946982425 0.0 30-31 5.843766892138672E-5 0.0 0.0 0.005113296030621339 0.0 32-33 5.843766892138672E-5 0.0 0.0 0.008298148986836914 0.0 34-35 5.843766892138672E-5 0.0 0.0 0.012125816301187746 0.0 36-37 5.843766892138672E-5 0.0 0.0 0.01817411503455127 0.0 38-39 5.843766892138672E-5 0.0 0.0 0.026793671200455817 0.0 40-41 5.843766892138672E-5 0.0 0.0 0.03827667314350831 0.0 42-43 5.843766892138672E-5 0.0 0.0 0.05428859442796827 0.0 44-45 5.843766892138672E-5 0.0 0.0 0.06831363496910109 0.0 46-47 5.843766892138672E-5 0.0 0.0 0.0964221537202881 0.0 48-49 5.843766892138672E-5 0.0 0.0 0.13110491022513113 0.0 50-51 5.843766892138672E-5 0.0 0.0 0.14983418311443558 0.0 52-53 5.843766892138672E-5 0.0 0.0 0.18174115034551272 0.0 54-55 5.843766892138672E-5 0.0 0.0 0.2333123931686365 0.0 56-57 5.843766892138672E-5 0.0 0.0 0.31191105786790163 0.0 58-59 5.843766892138672E-5 0.0 0.0 0.41587167087904864 0.0 60-61 5.843766892138672E-5 0.0 0.0 0.525997457961402 0.0 62-63 5.843766892138672E-5 0.0 0.0 0.6380224692837002 1.1687533784277345E-4 64-65 5.843766892138672E-5 0.0 0.0 0.7647445543397273 1.1687533784277345E-4 66-67 5.843766892138672E-5 0.0 0.0 0.9297140937048021 1.1687533784277345E-4 68-69 5.843766892138672E-5 0.0 0.0 1.1216526172770966 1.1687533784277345E-4 70-71 5.843766892138672E-5 0.0 0.0 1.2516764306271821 1.1687533784277345E-4 72-73 5.843766892138672E-5 0.0 0.0 1.4264050607021286 1.1687533784277345E-4 74-75 5.843766892138672E-5 0.0 0.0 1.6245379771800903 1.1687533784277345E-4 76-77 5.843766892138672E-5 0.0 0.0 1.8463673684056743 1.1687533784277345E-4 78-79 5.843766892138672E-5 0.0 0.0 2.0890297886017324 1.1687533784277345E-4 80-81 5.843766892138672E-5 0.0 0.0 2.4270917033119552 1.1687533784277345E-4 82-83 5.843766892138672E-5 0.0 0.0 2.806527487618519 1.1687533784277345E-4 84-85 5.843766892138672E-5 0.0 0.0 3.288609037385499 1.1687533784277345E-4 86-87 5.843766892138672E-5 0.0 0.0 3.859282093237301 1.1687533784277345E-4 88-89 5.843766892138672E-5 0.0 0.0 4.52716621133983 1.1687533784277345E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3440 0.0 43.388454 1 GTATCAA 5465 0.0 38.09666 1 ATCAACG 6665 0.0 30.999117 3 TCAACGC 6735 0.0 30.67693 4 TATCAAC 6860 0.0 30.187183 2 CAACGCA 6865 0.0 30.09601 5 AACGCAG 7115 0.0 29.038525 6 ACGCAGA 8135 0.0 25.285217 7 CGCAGTA 95 0.0012273499 24.998077 2 GTACATG 5820 0.0 24.910349 1 CGCAGAG 8215 0.0 24.807678 8 TACATGG 5945 0.0 24.047771 2 ACATGGG 5820 0.0 23.421738 3 GCAGAGT 9130 0.0 21.905224 9 GGACCGA 350 0.0 21.712616 6 GTACTTT 4960 0.0 21.162687 14-15 GAGTACT 5020 0.0 20.724152 12-13 GTGTAGG 620 0.0 19.933548 1 AGAGTAC 7810 0.0 19.76827 10-11 CATGGGG 3660 0.0 19.465715 4 >>END_MODULE