##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937383_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 982680 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.800817153091547 33.0 31.0 34.0 30.0 34.0 2 31.88929865266414 33.0 31.0 34.0 30.0 34.0 3 31.91875686897057 33.0 31.0 34.0 30.0 34.0 4 35.53574917572353 37.0 35.0 37.0 33.0 37.0 5 35.53668437334636 37.0 35.0 37.0 33.0 37.0 6 35.60241380713966 37.0 35.0 37.0 33.0 37.0 7 35.5669373549884 37.0 35.0 37.0 33.0 37.0 8 35.56999023079741 37.0 35.0 37.0 33.0 37.0 9 37.154656653234014 39.0 37.0 39.0 34.0 39.0 10-11 37.04242988561892 39.0 37.0 39.0 33.0 39.0 12-13 36.9091245369805 39.0 37.0 39.0 33.0 39.0 14-15 37.91674095331135 40.0 37.5 41.0 33.0 41.0 16-17 37.86855079985346 40.0 37.5 41.0 33.0 41.0 18-19 37.89721883013799 40.0 38.0 41.0 33.0 41.0 20-21 37.820411018846414 40.0 37.5 41.0 33.0 41.0 22-23 37.920220214108355 40.0 38.0 41.0 33.0 41.0 24-25 37.88120293483128 40.0 38.0 41.0 33.0 41.0 26-27 37.74369479382912 40.0 37.0 41.0 33.0 41.0 28-29 37.60707402206212 40.0 37.0 41.0 33.0 41.0 30-31 37.353914295599786 39.0 37.0 41.0 32.0 41.0 32-33 37.103489945862336 39.0 36.0 41.0 32.0 41.0 34-35 37.083363353278784 39.0 36.0 41.0 32.0 41.0 36-37 36.890433304839824 39.0 36.0 41.0 31.0 41.0 38-39 36.69724477958236 39.0 35.0 41.0 31.0 41.0 40-41 36.429542679203806 39.0 35.0 40.5 30.0 41.0 42-43 36.16812085317703 39.0 35.0 40.0 30.0 41.0 44-45 35.882670859282776 39.0 35.0 40.0 29.5 41.0 46-47 35.36115571701876 38.5 35.0 40.0 26.5 41.0 48-49 34.9356799772052 38.0 35.0 40.0 24.5 41.0 50-51 33.95594496682541 37.5 33.5 39.5 23.5 40.5 52-53 34.009481214637525 38.0 33.5 39.5 23.0 40.5 54-55 34.326908047380634 38.0 34.0 40.0 23.0 41.0 56-57 34.105953107827574 38.0 34.0 40.0 22.5 41.0 58-59 33.87416656490414 38.0 33.5 40.0 20.5 41.0 60-61 33.514226401269994 38.0 33.0 40.0 18.5 41.0 62-63 33.090666341026576 37.0 32.5 40.0 16.0 41.0 64-65 32.610742052346644 36.5 32.0 39.5 12.0 41.0 66-67 32.17155482964953 36.0 31.0 39.0 8.0 40.5 68-69 31.711721007856067 36.0 31.0 39.0 7.0 40.0 70-71 31.195993609313305 35.0 30.5 38.5 2.0 40.0 72-73 30.62223867383075 35.0 29.5 37.5 2.0 40.0 74-75 30.06040470956975 34.5 29.0 37.0 2.0 39.0 76-77 29.470646599096348 34.0 29.0 36.5 2.0 39.0 78-79 28.8957539585623 34.0 28.0 36.0 2.0 38.5 80-81 28.283021431188182 34.0 26.5 35.5 2.0 37.5 82-83 27.688727764887858 33.5 26.0 35.0 2.0 37.0 84-85 27.08288354296414 33.0 25.5 35.0 2.0 36.5 86-87 26.41350897545488 32.5 24.5 35.0 2.0 36.0 88-89 25.733810090772174 32.0 21.5 35.0 2.0 35.5 90-91 25.078088492693453 31.5 19.0 34.0 2.0 35.0 92-93 24.398198803272685 31.0 13.5 34.0 2.0 35.0 94-95 23.605168009932022 31.0 4.5 34.0 2.0 35.0 96-97 22.648844486506288 30.5 2.0 34.0 2.0 35.0 98-99 21.415767085928277 29.5 2.0 34.0 2.0 35.0 100-101 18.372973399275452 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1871035595635746 1101 2 0.10952091025095001 1101 3 0.10580944122821023 1101 4 0.15333673508601464 1101 5 0.0840636377570192 1101 6 0.0399963705028199 1101 7 0.16277968708833868 1101 8 0.15817668280192265 1101 9 0.07598378721856136 1101 10-11 0.26694288500926433 1101 12-13 0.3519986243889548 1101 14-15 0.41029174781405686 1101 16-17 0.45836338377429087 1101 18-19 0.37518551794168076 1101 20-21 0.4427605783084587 1101 22-23 0.4751128128095061 1101 24-25 0.35944088795812235 1101 26-27 0.39945483864610054 1101 28-29 0.28832008224765104 1101 30-31 0.3738100438101313 1101 32-33 0.25373362247030684 1101 34-35 0.3368970624176981 1101 36-37 0.5060371831577868 1101 38-39 0.41771697586427337 1101 40-41 0.533498994085285 1101 42-43 0.7567325483608087 1101 44-45 0.7703116241412147 1101 46-47 0.9251867109504133 1101 48-49 0.9663381409589107 1101 50-51 0.7257842133737213 1101 52-53 0.8169357807471584 1101 54-55 0.7802500892259374 1101 56-57 0.8401863607125719 1101 58-59 0.8060659142093485 1101 60-61 0.9322280299401271 1101 62-63 0.8145284190987852 1101 64-65 0.7403638759114415 1101 66-67 0.8195578183506171 1101 68-69 0.8398478909208684 1101 70-71 0.8819421165531232 1101 72-73 1.0644907187619381 1101 74-75 1.127716675986644 1101 76-77 1.1268022581880928 1101 78-79 1.0571026173354774 1101 80-81 1.254711045724303 1101 82-83 1.3290069752859317 1101 84-85 1.684867965335755 1101 86-87 1.6964125074211331 1101 88-89 1.6434409668087504 1101 90-91 1.7148378292179842 1101 92-93 1.876903432447694 1101 94-95 1.9375534531433125 1101 96-97 1.9720990072145774 1101 98-99 2.368998646765519 1101 100-101 2.2060542930351623 1102 1 0.5419916701194332 1102 2 0.5053643187634407 1102 3 0.39372508816462215 1102 4 0.38269177707203283 1102 5 0.40053905920481725 1102 6 0.34792315509182714 1102 7 0.3001172019263265 1102 8 0.34166462433901756 1102 9 0.4198417137589061 1102 10-11 0.5499345171150125 1102 12-13 0.4543563384346072 1102 14-15 0.5985473862506794 1102 16-17 0.7899198218748396 1102 18-19 0.7526041715557952 1102 20-21 0.6749159094826425 1102 22-23 0.6822096468829102 1102 24-25 0.7824469794725033 1102 26-27 0.7819489400866715 1102 28-29 0.6909059227740499 1102 30-31 0.7205012328522429 1102 32-33 0.8481740775508797 1102 34-35 1.0879204563730767 1102 36-37 0.9986890191607358 1102 38-39 0.8185894253174055 1102 40-41 1.0223821024673398 1102 42-43 1.1879990655484463 1102 44-45 1.1274223802803576 1102 46-47 1.2539636208380074 1102 48-49 1.2548815020875281 1102 50-51 0.9704132269587262 1102 52-53 0.879081928242897 1102 54-55 1.1458704027410818 1102 56-57 1.1204509776603615 1102 58-59 1.2318381003644703 1102 60-61 1.1512538761330475 1102 62-63 1.1360304390195566 1102 64-65 1.2266848357035371 1102 66-67 1.3633842370579643 1102 68-69 1.3206757409142718 1102 70-71 1.409195170858947 1102 72-73 1.5921797535475655 1102 74-75 1.7694164380902926 1102 76-77 1.7628867871093554 1102 78-79 1.62898444097954 1102 80-81 1.7684499263902147 1102 82-83 2.027100873513188 1102 84-85 1.728967940422585 1102 86-87 1.9699383560565273 1102 88-89 2.2360788807459606 1102 90-91 2.198120359223701 1102 92-93 2.349012894078971 1102 94-95 2.396257430946168 1102 96-97 2.558459779229757 1102 98-99 2.680976263991756 1102 100-101 2.6220541660268424 1103 1 0.2969491409360536 1103 2 0.28323506150165656 1103 3 0.167243241935374 1103 4 0.22571602172411076 1103 5 0.26013880390170385 1103 6 0.3213083537289876 1103 7 0.27458414693923316 1103 8 0.2951339852216748 1103 9 0.3003969797514685 1103 10-11 0.36995039126301066 1103 12-13 0.279261102690711 1103 14-15 0.5090285302134774 1103 16-17 0.4663235775373167 1103 18-19 0.41662225125938335 1103 20-21 0.24032133119913368 1103 22-23 0.27547666724314723 1103 24-25 0.3764372977052872 1103 26-27 0.15886531368511214 1103 28-29 0.1943957664283218 1103 30-31 0.129096864535434 1103 32-33 0.44154625499751887 1103 34-35 0.4136335585802797 1103 36-37 0.6474355466908648 1103 38-39 0.5904762726348025 1103 40-41 0.6859530359513712 1103 42-43 0.5404371554568428 1103 44-45 0.6229975529665808 1103 46-47 0.6728952797047612 1103 48-49 0.7501713790263125 1103 50-51 0.5837721088920063 1103 52-53 0.6608251949889308 1103 54-55 0.8874418336638499 1103 56-57 0.9681473973816139 1103 58-59 1.0773929731609755 1103 60-61 1.0053141284003289 1103 62-63 0.8546309902255658 1103 64-65 0.9483838633205295 1103 66-67 1.0365354383176637 1103 68-69 0.9609932421624414 1103 70-71 1.2013508179773673 1103 72-73 1.163149356748729 1103 74-75 1.0697194733810136 1103 76-77 0.863946404776474 1103 78-79 1.1919342161262563 1103 80-81 1.0484356981792224 1103 82-83 1.2757269733191485 1103 84-85 1.6237994239056022 1103 86-87 1.783449578603669 1103 88-89 1.703884352937859 1103 90-91 1.6125595244776036 1103 92-93 1.9741041064213007 1103 94-95 1.7975647621324704 1103 96-97 1.9545261726708816 1103 98-99 1.8019933250432203 1103 100-101 1.7488923576175708 1104 1 0.22934671938007156 1104 2 0.17455281170992265 1104 3 0.06622053838478337 1104 4 0.08804514029099408 1104 5 0.28105623258851864 1104 6 0.20830370844635837 1104 7 0.3845169747086956 1104 8 0.20437343481128778 1104 9 0.2239985662881523 1104 10-11 0.28312204040663147 1104 12-13 0.2326717078260785 1104 14-15 0.4446915609043387 1104 16-17 0.2946315123667915 1104 18-19 0.33964878653407027 1104 20-21 0.4004475010553321 1104 22-23 0.4114697061476349 1104 24-25 0.5343691031613886 1104 26-27 0.5190883496826402 1104 28-29 0.39265383421706446 1104 30-31 0.48493751241676364 1104 32-33 0.5042912248841489 1104 34-35 0.7128431191611284 1104 36-37 0.7233423132737045 1104 38-39 0.851798295488571 1104 40-41 0.6378813842714806 1104 42-43 0.6470221020877958 1104 44-45 0.6262473973759484 1104 46-47 0.8316011363506846 1104 48-49 0.9290747528554704 1104 50-51 0.7149652500278307 1104 52-53 0.779726676367801 1104 54-55 0.8983180576597292 1104 56-57 0.8977614155608435 1104 58-59 1.1413182857566895 1104 60-61 1.0855769448736154 1104 62-63 1.0564861768075033 1104 64-65 1.0134665946140515 1104 66-67 1.023591663601188 1104 68-69 0.9169103684474074 1104 70-71 0.9826284365335276 1104 72-73 1.2412958480093117 1104 74-75 1.1336457614665783 1104 76-77 1.1280251619243806 1104 78-79 1.1910513978190451 1104 80-81 1.196557977781925 1104 82-83 1.2443352224514506 1104 84-85 1.2755656458139342 1104 86-87 1.3168893703951134 1104 88-89 1.35308601205098 1104 90-91 1.6858368581907257 1104 92-93 1.9723155457955812 1104 94-95 1.871350326053392 1104 96-97 2.162418178141426 1104 98-99 2.4757061174219146 1104 100-101 2.5031547604831665 1105 1 0.20389766645779162 1105 2 0.10397473229097898 1105 3 0.09664359780207832 1105 4 0.1398381832114879 1105 5 0.16076113380935908 1105 6 0.08122840506077011 1105 7 0.20887597122688817 1105 8 0.11853149298988086 1105 9 0.4060241584157751 1105 10-11 0.20143886733617222 1105 12-13 0.3444572225205249 1105 14-15 0.42687972046432066 1105 16-17 0.3116074700550868 1105 18-19 0.2960105216365392 1105 20-21 0.4835129294056344 1105 22-23 0.48467018165496967 1105 24-25 0.562689252496817 1105 26-27 0.5173752941638909 1105 28-29 0.4684762256963424 1105 30-31 0.59132315731879 1105 32-33 0.5782200370568304 1105 34-35 0.4965914651599306 1105 36-37 0.522212887320137 1105 38-39 0.47649894287760475 1105 40-41 0.3862115392338694 1105 42-43 0.5319554715258832 1105 44-45 0.5821136522947512 1105 46-47 0.5969600618116431 1105 48-49 0.6959172381687111 1105 50-51 0.8419142827852113 1105 52-53 0.6963861412876184 1105 54-55 0.7815724598475242 1105 56-57 0.7641176389263862 1105 58-59 0.778336320851281 1105 60-61 0.836010009917878 1105 62-63 0.6546680718079543 1105 64-65 0.7877215979076482 1105 66-67 0.7887945512136056 1105 68-69 1.0660743896723979 1105 70-71 0.946392127054299 1105 72-73 1.0379694091483032 1105 74-75 0.9931382399621604 1105 76-77 1.027575196780834 1105 78-79 1.088728018260099 1105 80-81 1.0971441039526901 1105 82-83 1.2478847234184052 1105 84-85 1.4510871002979329 1105 86-87 1.4412001632210227 1105 88-89 1.6511159021811288 1105 90-91 1.5406712468200325 1105 92-93 1.578864430293006 1105 94-95 1.5967578008904901 1105 96-97 1.5194995117897179 1105 98-99 1.4985572601951027 1105 100-101 1.5451178990620633 1106 1 0.22017428023079688 1106 2 -0.388110297107481 1106 3 0.17459020584016116 1106 4 0.3103896667143431 1106 5 0.09669279963991784 1106 6 -0.004621389503519424 1106 7 0.11656513649910494 1106 8 0.12079066857597098 1106 9 0.3118996983789444 1106 10-11 -0.21180396429857495 1106 12-13 0.04600868021064741 1106 14-15 -0.013273450069213766 1106 16-17 0.04219488937626181 1106 18-19 0.12216679194302316 1106 20-21 0.2536134756255777 1106 22-23 0.2513179938011021 1106 24-25 0.3049058707365404 1106 26-27 0.25022750053559406 1106 28-29 0.3582687474099018 1106 30-31 0.300120137902006 1106 32-33 0.23494823904261608 1106 34-35 0.3294079826177594 1106 36-37 0.4254190536461948 1106 38-39 0.4832315779203 1106 40-41 0.3655192640880571 1106 42-43 0.26374654997079716 1106 44-45 0.37099520887009163 1106 46-47 0.33023705604193765 1106 48-49 0.41207365437327326 1106 50-51 0.38207833955976866 1106 52-53 0.3370936122791477 1106 54-55 0.5382307429863076 1106 56-57 0.3526373682742303 1106 58-59 0.20792927644327364 1106 60-61 0.19639789678626585 1106 62-63 0.2028256596863045 1106 64-65 0.5098519791617662 1106 66-67 0.7756064874718547 1106 68-69 0.7273731294497061 1106 70-71 0.5977265894142789 1106 72-73 0.7374917612946312 1106 74-75 0.6402270683220515 1106 76-77 0.7918061511899275 1106 78-79 1.0133228424697514 1106 80-81 0.9832302533994515 1106 82-83 0.8969695496818559 1106 84-85 0.8833516741128236 1106 86-87 0.7318190291889515 1106 88-89 1.002217376537299 1106 90-91 1.1497059290371894 1106 92-93 1.509186315116576 1106 94-95 1.6877678981596098 1106 96-97 1.5486987670361572 1106 98-99 1.702140629161235 1106 100-101 1.6273892627489275 1107 1 0.10732526326646408 1107 2 0.35315743064380456 1107 3 0.10945069433290655 1107 4 0.3106278629022512 1107 5 0.24979935432261158 1107 6 0.18463024571331488 1107 7 0.11849214365928873 1107 8 0.1050125530889261 1107 9 0.09153845004036043 1107 10-11 0.2860975272859534 1107 12-13 0.2497349022761881 1107 14-15 0.45308610231251834 1107 16-17 0.5377257334006345 1107 18-19 0.4074216142248446 1107 20-21 0.36212791746045525 1107 22-23 0.3839301484761748 1107 24-25 0.4829247692220875 1107 26-27 0.39307851834790597 1107 28-29 0.31151679062822524 1107 30-31 0.4247807083342323 1107 32-33 0.38759507508265045 1107 34-35 0.41640616279887865 1107 36-37 0.26199205363190003 1107 38-39 0.3225009587531318 1107 40-41 0.10784934683740488 1107 42-43 -0.12517482623150755 1107 44-45 -0.3170023234173982 1107 46-47 -0.13177184296765176 1107 48-49 -0.14777432881960095 1107 50-51 -0.25410497146252453 1107 52-53 -0.17921568070692473 1107 54-55 -0.07413830865939275 1107 56-57 0.06264720577679128 1107 58-59 0.41794685532194364 1107 60-61 0.26536462554273754 1107 62-63 0.21006205987495008 1107 64-65 -0.15072321699279456 1107 66-67 0.16833483424739626 1107 68-69 0.4691678662667407 1107 70-71 0.30021478679316616 1107 72-73 0.33230396742008494 1107 74-75 0.36754960025225003 1107 76-77 0.46029839314008925 1107 78-79 0.5701070271956574 1107 80-81 0.4732843715733459 1107 82-83 0.5733639501658736 1107 84-85 0.7647448597962807 1107 86-87 0.7208983294638287 1107 88-89 0.7999185451277988 1107 90-91 0.6115087883442314 1107 92-93 0.7334206334705478 1107 94-95 0.7703213374743676 1107 96-97 0.9964665114611755 1107 98-99 1.1624607171985488 1107 100-101 1.317962291318178 1108 1 0.284114327098667 1108 2 0.2507161187583904 1108 3 0.36749082392990573 1108 4 0.3164085711384814 1108 5 0.20292559771487362 1108 6 0.37034047137124304 1108 7 0.3949430047972413 1108 8 0.34933131623336067 1108 9 0.33218814025806154 1108 10-11 0.3091009945740737 1108 12-13 0.3375496581449937 1108 14-15 0.40038900687682855 1108 16-17 0.39256998963890766 1108 18-19 0.6222186926082927 1108 20-21 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-0.2721335802988776 2316 56-57 -0.26494133036138834 2316 58-59 -0.3742103385408413 2316 60-61 -0.5519807377690427 2316 62-63 -0.35666089679782687 2316 64-65 -0.0408201966318984 2316 66-67 -0.006013809967598149 2316 68-69 -0.06180530752463298 2316 70-71 -0.08887862245125788 2316 72-73 -0.0755726127208014 2316 74-75 -0.09558848717416879 2316 76-77 0.02014834006665467 2316 78-79 0.20801352145408103 2316 80-81 0.5326421830179093 2316 82-83 0.342656143546165 2316 84-85 0.528534303007703 2316 86-87 0.9130868849014426 2316 88-89 0.7643038582610586 2316 90-91 0.8727434694211382 2316 92-93 1.2001242907126546 2316 94-95 1.5716964219093796 2316 96-97 1.7032357867675536 2316 98-99 1.831863882305175 2316 100-101 2.3300919834922595 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3678.0 3 516.0 4 482.0 5 538.0 6 639.0 7 782.0 8 1001.0 9 1231.0 10 1716.0 11 2539.0 12 3710.0 13 5231.0 14 6035.0 15 7926.0 16 10656.0 17 14776.0 18 12299.0 19 7910.0 20 7701.0 21 8329.0 22 9110.0 23 10143.0 24 11424.0 25 13343.0 26 15418.0 27 17851.0 28 21198.0 29 25370.0 30 30332.0 31 36830.0 32 45831.0 33 57908.0 34 75348.0 35 100246.0 36 130323.0 37 159657.0 38 113019.0 39 11631.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.83060802378749 19.007031553727458 14.681671940416996 20.480688482068057 2 13.820659685917281 21.78473245844448 41.46208106577988 22.93252678985836 3 20.3795302947924 27.54619169105504 30.95529303276389 21.11898498138867 4 11.157195507213409 16.77215867766572 40.737733429763736 31.33291238535713 5 11.926725294319864 39.6412975066076 34.62513426861476 13.806842930457783 6 27.555658442614007 37.32325493407466 19.691631168931824 15.429455454379504 7 25.36389660267988 36.023851506322366 21.229553921353666 17.38269796964409 8 24.30531405301713 33.106182136370265 21.60020233521765 20.98830147539496 9 26.361771764329113 17.900616949643485 22.246394327612602 33.491216958414796 10-11 22.983828399165027 26.81653196707642 28.378153013362517 21.821486620396037 12-13 27.28391869722176 24.81991843611943 27.992209767165054 19.90395309949376 14-15 23.849202767634644 25.398182751796572 29.486098851816102 21.266515628752682 16-17 19.52693224909864 29.389317453251813 30.45172876388373 20.632021533765823 18-19 18.98723657658411 29.107121188792007 33.07895539174849 18.826686842875397 20-21 20.86443326696528 27.341156435704146 31.483695638318753 20.310714659011822 22-23 19.78586968326069 27.314132407158613 30.7998301151923 22.100167794388398 24-25 20.18924308375062 27.077424160767904 32.26952506185989 20.463807693621586 26-27 21.386880368070006 26.02630003783078 32.746208509783486 19.840611084315725 28-29 19.51080219608195 26.658776653652055 33.942387267742205 19.888033882523793 30-31 19.738796075311587 28.282081879933905 32.822858658180856 19.15626338657365 32-33 19.10384165475997 28.12447414233571 32.03858890143299 20.73309530147133 34-35 19.980368110672863 26.794112472878673 32.17569776068982 21.049821655758645 36-37 20.85760757060476 26.787897637755126 32.64231849770812 19.712176293931993 38-39 21.591989579111317 26.006388147513693 32.916548768892326 19.485073504482664 40-41 20.10876408939517 26.868818802820098 33.64534056455975 19.377076543224984 42-43 19.146630907797693 28.39867739772649 32.284140244788496 20.17055144968732 44-45 19.86472658526401 28.62588784012734 30.912820230442023 20.596565344166624 46-47 19.699263455512867 28.101785400153478 29.77944457050202 22.419506573831637 48-49 20.452519698048246 27.70571534051159 30.453906669087065 21.387858292353098 50-51 21.620166174812475 28.32534484613812 30.280675612344442 19.773813366704964 52-53 20.033374252152065 28.750255636367534 30.833953405586414 20.382416705893995 54-55 19.19338973448692 28.1153002619487 31.615683470919713 21.075626532644662 56-57 20.50792750913732 29.526001281697674 29.168359707381384 20.79771150178362 58-59 20.137375129738647 28.30086723530767 30.04252572852456 21.519231906429123 60-61 20.257128014963364 27.327307272515018 30.93988502190314 21.47567969061848 62-63 22.069296366026904 27.286024031400185 30.23975830951248 20.404921293060422 64-65 21.258264202652597 27.827467357387224 31.02145519297031 19.892813246989867 66-67 19.925160843011103 28.009829011898812 31.159242228045315 20.90576791704477 68-69 19.793101022310566 29.336586564515482 30.651626760035626 20.21868565313833 70-71 19.849990976210844 29.121348041939715 29.376512249411412 21.65214873243802 72-73 19.90037094514539 28.166085131808906 29.263523999274017 22.67001992377169 74-75 20.29459284324257 28.452207305017442 29.87515671052437 21.37804314121562 76-77 20.994699369186183 27.333429869126018 30.346825230105303 21.3250455315825 78-79 20.108114833004294 27.314801595718645 31.29634803961559 21.280735531661474 80-81 19.789904527086346 29.04786085814477 30.275304196913012 20.886930417855872 82-83 19.488201721628435 28.958687545086327 29.77662674004532 21.77648399323992 84-85 19.987775933576117 27.449248204622105 29.760962917952966 22.80201294384882 86-87 20.596889385064763 27.708534055167284 30.208037029673697 21.486539530094255 88-89 21.48541276335411 26.29347812233141 30.57780496819995 21.64330414611453 90-91 19.880684184740687 26.852736937788457 31.59314958779353 21.673429289677326 92-93 19.46714468452402 28.146717221541596 31.093236424593652 21.292901669340733 94-95 19.333706175914745 28.496202154771815 30.157220953073328 22.012870716240116 96-97 18.999047605451693 27.749758842279793 29.541004969990393 23.710188582278118 98-99 19.8863928954006 27.246840267350425 29.619781769531862 23.246985067717116 100-101 21.074769666360964 26.54695553807559 30.79626898503322 21.582005810530223 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1857.0 1 1138.5 2 493.0 3 557.0 4 495.0 5 528.0 6 928.0 7 1845.5 8 2904.5 9 3247.5 10 2676.5 11 2085.5 12 2001.5 13 2066.5 14 2128.0 15 2187.0 16 2231.5 17 2332.0 18 2488.5 19 2688.5 20 2946.5 21 3310.0 22 3832.0 23 4388.5 24 5091.0 25 6201.0 26 7611.0 27 9398.5 28 11152.5 29 13099.5 30 15493.5 31 17609.0 32 19955.5 33 23028.0 34 26182.0 35 28409.0 36 30065.5 37 31579.0 38 31940.0 39 31336.5 40 30657.0 41 30342.0 42 30667.0 43 31393.5 44 32744.5 45 33266.0 46 34359.0 47 42259.0 48 71573.0 49 70034.5 50 40163.5 51 30133.0 52 22887.5 53 19239.0 54 16908.5 55 14727.0 56 13137.0 57 11689.0 58 10099.0 59 8594.0 60 7326.5 61 6143.5 62 4857.0 63 3277.5 64 2091.0 65 1500.0 66 1022.5 67 702.5 68 497.0 69 361.0 70 292.0 71 224.5 72 177.5 73 162.5 74 153.5 75 135.0 76 99.0 77 61.0 78 40.0 79 29.5 80 19.5 81 15.0 82 9.5 83 4.0 84 5.0 85 3.5 86 2.5 87 3.5 88 3.0 89 2.5 90 2.5 91 1.5 92 0.5 93 0.0 94 1.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27160418447510887 2 0.2406683762771197 3 0.24046485122318556 4 0.2394472259535149 5 0.241075426384988 6 0.21380306915781333 7 0.21502421948141817 8 0.2166524199128913 9 0.22499694712419097 10-11 0.23288354296413888 12-13 0.22606545365734523 14-15 0.21690682623030896 16-17 0.21171693735498842 18-19 0.20220214108356738 20-21 0.20734114869540443 22-23 0.20510237310212887 24-25 0.20723938616843732 26-27 0.20321976635323807 28-29 0.21527862579883583 30-31 0.22591280986689463 32-33 0.21767004518256197 34-35 0.217008588757276 36-37 0.20810436764765744 38-39 0.19920014653803886 40-41 0.19675784589082917 42-43 0.20734114869540443 44-45 0.20347417267065573 46-47 0.21242927504375786 48-49 0.21634713233199004 50-51 0.22453901575283916 52-53 0.23237473032930353 54-55 0.21822973908088086 56-57 0.19838604632230228 58-59 0.18948182521268367 60-61 0.19177148206944275 62-63 0.19553669556722433 64-65 0.19014328163796962 66-67 0.19436642650710304 68-69 0.19451907029755364 70-71 0.19731753978914804 72-73 0.19762282737004924 74-75 0.2005230593886107 76-77 0.20779908006675624 78-79 0.2092237554442952 80-81 0.20179509097569912 82-83 0.19563845809419136 84-85 0.18602189929580332 86-87 0.1877518622542435 88-89 0.19767370863353279 90-91 0.19553669556722433 92-93 0.19981072169984126 94-95 0.19604550820205968 96-97 0.20291447877233687 98-99 0.20550942320999716 100-101 0.19075385679977205 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 982680.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.868767106842114 #Duplication Level Percentage of deduplicated Percentage of total 1 78.79699380054595 44.810878891625286 2 13.07642113753965 14.872798965234596 3 4.05221888550895 6.913340761978673 4 1.6190697962416198 3.6829801268874793 5 0.7941762777134774 2.2581912889533253 6 0.43611916774459936 1.4880935628778453 7 0.2700761018668562 1.0751226456733007 8 0.17562444201744754 0.7990036393087455 9 0.13232907044461473 0.6772851979721728 >10 0.5706342702824472 5.775894198733536 >50 0.03655877856885896 1.4088222468015634 >100 0.029206568997437104 3.3318691163479244 >500 0.00447953496952214 1.728092461967995 >1k 0.004837897767083912 5.601346932860468 >5k 8.959069939044282E-4 3.328812108471635 >10k+ 3.583627975617713E-4 2.247467854305448 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 11572 1.17759596206293 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 10484 1.0668783327227582 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8216 0.8360809215614442 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 8060 0.8202059673545814 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6009 0.6114910245451215 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 5256 0.5348638417389181 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 5127 0.521736475760166 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 4793 0.4877477917531648 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4154 0.4227215370212073 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 3973 0.4043025196401677 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 3200 0.3256400862946229 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2804 0.2853421256156633 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2569 0.26142793177840196 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2306 0.2346643871860626 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2243 0.2282533479871372 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1983 0.20179509097569912 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1982 0.20169332844873203 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1978 0.20128627834086377 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1965 0.19996336549029187 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1923 0.19568933935767494 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1836 0.18683599951153987 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1690 0.1719786705743477 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1655 0.16841698213050027 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1520 0.15467904098994587 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1444 0.14694508894044858 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1408 0.14328163796963408 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1405 0.14297635038873285 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1399 0.14236577522693045 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1319 0.13422477306956487 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1159 0.11794276875483371 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1138 0.11580575568852526 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1083 0.11020881670533642 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1031 0.1049171653030488 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1010 0.10278015223674035 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 5.0881263483534825E-5 0.0 0.0 0.0 12-13 0.0 1.0176252696706965E-4 0.0 1.526437904506045E-4 0.0 14-15 0.0 1.0176252696706965E-4 0.0 2.035250539341393E-4 0.0 16-17 0.0 1.0176252696706965E-4 0.0 2.035250539341393E-4 0.0 18-19 0.0 1.0176252696706965E-4 0.0 4.070501078682786E-4 0.0 20-21 0.0 1.526437904506045E-4 0.0 6.614564252859527E-4 0.0 22-23 0.0 2.035250539341393E-4 0.0 0.001119387796637766 0.0 24-25 0.0 2.035250539341393E-4 0.0 0.0015264379045060448 0.0 26-27 0.0 2.035250539341393E-4 0.0 0.002238775593275532 0.0 28-29 0.0 2.035250539341393E-4 0.0 0.0054442951927382265 0.0 30-31 0.0 2.035250539341393E-4 0.0 0.011855334391663613 0.0 32-33 0.0 2.035250539341393E-4 0.0 0.0192839988602597 0.0 34-35 0.0 2.035250539341393E-4 0.0 0.026814425855822853 0.0 36-37 0.0 2.035250539341393E-4 0.0 0.03510807180363903 0.0 38-39 0.0 2.035250539341393E-4 0.0 0.04487727439247771 0.0 40-41 0.0 2.035250539341393E-4 0.0 0.05993812838360402 0.0 42-43 0.0 2.035250539341393E-4 0.0 0.0775939268123906 0.0 44-45 0.0 2.035250539341393E-4 0.0 0.09631823177433142 0.0 46-47 0.0 2.035250539341393E-4 0.0 0.11646721211381121 0.0 48-49 0.0 2.035250539341393E-4 0.0 0.13854968046566535 0.0 50-51 0.0 2.035250539341393E-4 0.0 0.17014694508894046 0.0 52-53 0.0 2.035250539341393E-4 0.0 0.2500305287580901 0.0 54-55 0.0 2.035250539341393E-4 0.0 0.3575935197622827 0.0 56-57 0.0 2.035250539341393E-4 0.0 0.43635771563479464 0.0 58-59 0.0 2.035250539341393E-4 0.0 0.5688016444824358 0.0 60-61 0.0 2.035250539341393E-4 0.0 0.7497862986933692 0.0 62-63 0.0 2.035250539341393E-4 0.0 0.8566878332722758 0.0 64-65 0.0 2.035250539341393E-4 0.0 0.9346888101925347 0.0 66-67 0.0 2.035250539341393E-4 0.0 1.1501709610453048 0.0 68-69 0.0 2.035250539341393E-4 0.0 1.3688077502340539 0.0 70-71 0.0 2.035250539341393E-4 0.0 1.477846297879269 0.0 72-73 0.0 2.035250539341393E-4 0.0 1.730064720967151 0.0 74-75 0.0 3.05287580901209E-4 0.0 2.073258843163593 0.0 76-77 0.0 4.070501078682786E-4 0.0 2.3842451255749584 0.0 78-79 0.0 4.579313713518134E-4 0.0 2.713803069157813 0.0 80-81 0.0 5.088126348353482E-4 0.0 3.144563845809419 0.0 82-83 0.0 5.088126348353482E-4 0.0 3.604174298856189 0.0 84-85 0.0 5.088126348353482E-4 0.0 4.143006879146823 0.0 86-87 0.0 5.088126348353482E-4 0.0 4.853716367484838 0.0 88-89 0.0 5.088126348353482E-4 0.0 5.579995522448813 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCGCCG 60 0.003944656 31.676525 5 GAGTACT 5840 0.0 22.408653 12-13 AGTACTT 5955 0.0 21.975908 12-13 CATGGGT 975 0.0 21.928785 4 CTAGACT 305 9.094947E-12 21.808954 4 CATTCCG 110 0.0028744258 21.594328 9 GTACATG 9415 0.0 21.05338 1 TACATGG 9430 0.0 20.910538 2 GTATAGG 255 1.1814336E-8 20.504944 1 ACATGGG 9475 0.0 20.158281 3 ATGGGAT 2095 0.0 19.958479 5 GTACTTT 6565 0.0 19.93194 14-15 GTATAGC 555 0.0 19.698853 1 CATGGGG 1735 0.0 19.4431 4 GTTAAGC 150 7.706449E-4 19.004946 3 TAGCCCT 730 0.0 18.874777 4 TACCTGG 885 0.0 18.79116 2 TACTTTT 7030 0.0 18.681105 14-15 ATAGCCC 510 0.0 18.632301 3 GCTAGAC 230 2.0521147E-6 18.591797 3 >>END_MODULE