##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937383_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 982680 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.155093214474704 33.0 31.0 34.0 31.0 34.0 2 32.32249765946188 33.0 31.0 34.0 31.0 34.0 3 32.39137053771319 34.0 31.0 34.0 31.0 34.0 4 36.0083679325925 37.0 35.0 37.0 35.0 37.0 5 35.9542007571132 37.0 35.0 37.0 35.0 37.0 6 36.03031709203403 37.0 35.0 37.0 35.0 37.0 7 35.97885883502259 37.0 35.0 37.0 35.0 37.0 8 35.980922579069485 37.0 35.0 37.0 35.0 37.0 9 37.63484959498514 39.0 37.0 39.0 35.0 39.0 10-11 37.558692555053526 39.0 37.0 39.0 35.0 39.0 12-13 37.454710587373306 39.0 37.0 39.0 34.5 39.0 14-15 38.539385150812066 40.0 38.0 41.0 34.0 41.0 16-17 38.51864289494037 40.0 38.0 41.0 34.0 41.0 18-19 38.6143637806814 40.0 38.0 41.0 34.0 41.0 20-21 38.565112752879884 40.0 38.0 41.0 34.0 41.0 22-23 38.533629971099444 40.0 38.0 41.0 34.0 41.0 24-25 38.560910469328775 40.0 38.0 41.0 34.0 41.0 26-27 38.316238246428135 40.0 38.0 41.0 34.0 41.0 28-29 38.14408861480848 40.0 37.5 41.0 34.0 41.0 30-31 38.170881670533646 40.0 37.0 41.0 34.0 41.0 32-33 38.02963630072862 40.0 37.0 41.0 34.0 41.0 34-35 37.89780803516913 40.0 37.0 41.0 33.0 41.0 36-37 37.69073197785647 40.0 37.0 41.0 33.0 41.0 38-39 37.50752584768185 40.0 36.0 41.0 33.0 41.0 40-41 37.272950502706884 40.0 36.0 41.0 33.0 41.0 42-43 37.08538740994016 40.0 36.0 41.0 32.5 41.0 44-45 36.743898318883055 39.5 36.0 41.0 31.0 41.0 46-47 36.26231784507673 39.0 36.0 41.0 30.0 41.0 48-49 35.839080372043796 39.0 35.0 41.0 29.0 41.0 50-51 35.55376775756096 39.0 35.0 40.0 27.5 41.0 52-53 35.234986465583916 39.0 35.0 40.0 27.0 41.0 54-55 35.04428196360972 39.0 35.0 40.0 26.0 41.0 56-57 34.82482191557781 38.0 34.5 40.0 26.0 41.0 58-59 34.61519874221517 38.0 34.0 40.0 25.0 41.0 60-61 34.25283408637603 38.0 34.0 40.0 23.5 41.0 62-63 33.834671001750316 37.5 33.5 40.0 22.5 41.0 64-65 33.36765986892986 37.0 33.0 39.5 22.0 41.0 66-67 32.939032543656126 36.0 32.0 39.0 20.0 40.5 68-69 32.53977388366508 36.0 32.0 39.0 18.5 40.0 70-71 32.086919953596286 35.5 31.5 38.5 17.5 40.0 72-73 31.573932511092117 35.0 31.0 37.5 13.5 39.5 74-75 31.087142813530345 35.0 31.0 37.0 9.5 39.0 76-77 29.699108051451134 33.5 29.0 35.5 7.5 38.0 78-79 30.304587454715676 34.0 30.0 36.0 7.0 38.5 80-81 30.150145520413563 34.0 30.0 36.0 2.0 37.5 82-83 29.75862844466154 34.0 30.0 35.0 2.0 37.0 84-85 29.326608356738717 34.0 29.0 35.0 2.0 36.5 86-87 28.94170482761428 34.0 29.0 35.0 2.0 36.0 88-89 28.40848852118696 34.0 29.0 35.0 2.0 36.0 90-91 27.80587932999552 33.0 27.0 35.0 2.0 35.0 92-93 27.376346827044408 33.0 26.0 35.0 2.0 35.0 94-95 26.913003724508485 33.0 25.5 35.0 2.0 35.0 96-97 26.20179712622624 32.5 25.0 34.0 2.0 35.0 98-99 25.22518317254854 32.0 21.0 34.0 2.0 35.0 100-101 22.375250844628972 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1271947793457997 1101 2 0.21936343317684504 1101 3 0.1855524209341155 1101 4 0.0709250859530286 1101 5 0.08495734907243246 1101 6 0.11533466383580304 1101 7 0.055425285787904954 1101 8 0.14515741789823977 1101 9 0.17112347429191033 1101 10-11 0.14307552465103157 1101 12-13 0.18313936302921974 1101 14-15 0.31462844743701623 1101 16-17 0.12814338116182 1101 18-19 0.17052817541839005 1101 20-21 0.24792048422368396 1101 22-23 0.3191748578503777 1101 24-25 0.16909442717765444 1101 26-27 0.12597848861931027 1101 28-29 0.011589648220571291 1101 30-31 0.19318884087876143 1101 32-33 0.16454650618344147 1101 34-35 0.02486898280174188 1101 36-37 0.010568302783802608 1101 38-39 -0.09099161492883212 1101 40-41 0.031704182124478564 1101 42-43 0.14887259544263998 1101 44-45 0.14938252764526538 1101 46-47 0.07773882180163838 1101 48-49 0.05434082901375348 1101 50-51 0.0814988120575677 1101 52-53 0.06290348019982162 1101 54-55 0.03467797902804648 1101 56-57 0.03812790040234404 1101 58-59 0.08582503380478812 1101 60-61 0.08453539896191842 1101 62-63 0.027965549881862728 1101 64-65 0.1330621003408865 1101 66-67 0.054113965375599093 1101 68-69 0.2812251467224627 1101 70-71 0.13259620060265576 1101 72-73 0.3134343373444395 1101 74-75 0.06984701061876208 1101 76-77 0.2508888022454627 1101 78-79 0.40126097948929385 1101 80-81 0.42696426993985526 1101 82-83 0.23152503701342297 1101 84-85 0.2736179198307198 1101 86-87 0.3423599808428577 1101 88-89 0.5275006289728523 1101 90-91 0.6110987879533276 1101 92-93 0.7159150137049259 1101 94-95 0.720338100842639 1101 96-97 0.6903873953386572 1101 98-99 0.8183972308979754 1101 100-101 1.4868467859179084 1102 1 0.3393255392951886 1102 2 0.40562113305619363 1102 3 0.3196121441536732 1102 4 0.27525704661952943 1102 5 0.26539464848477934 1102 6 0.27405598315914403 1102 7 0.23917457136610665 1102 8 0.3406378757143784 1102 9 0.34947490631070366 1102 10-11 0.33515118252969955 1102 12-13 0.2539782529765162 1102 14-15 0.5251251965125334 1102 16-17 0.5252263420188328 1102 18-19 0.4326232753397363 1102 20-21 0.3420544096111868 1102 22-23 0.5545493614188217 1102 24-25 0.424973446868826 1102 26-27 0.5284238872544549 1102 28-29 0.37247890185130217 1102 30-31 0.4788623024904979 1102 32-33 0.4965926590526095 1102 34-35 0.5603079675167706 1102 36-37 0.7500339397060927 1102 38-39 0.551149012589967 1102 40-41 0.6277762056112479 1102 42-43 0.7140913672721894 1102 44-45 0.7631160348822945 1102 46-47 0.7145621971942617 1102 48-49 0.8681490757616075 1102 50-51 0.8524930429489217 1102 52-53 0.9151879202152529 1102 54-55 0.9845280115128645 1102 56-57 0.9703616347888726 1102 58-59 0.9741163322694462 1102 60-61 0.9841035706771635 1102 62-63 1.003864500109259 1102 64-65 0.9438319173023615 1102 66-67 0.8791771031649702 1102 68-69 0.7878759861985998 1102 70-71 0.8298743146268137 1102 72-73 0.8102322622239058 1102 74-75 0.8143966440043648 1102 76-77 0.8951507129390741 1102 78-79 0.9782841975870014 1102 80-81 0.9775624302732062 1102 82-83 0.9341863497909486 1102 84-85 1.1156659778246727 1102 86-87 1.2089035471387568 1102 88-89 1.422644270496754 1102 90-91 1.5761739426836563 1102 92-93 1.5702865423477448 1102 94-95 1.5151793648685121 1102 96-97 1.9094254538699502 1102 98-99 2.451690019267925 1102 100-101 3.2304695028212507 1103 1 0.2097144010565941 1103 2 0.16984797243651428 1103 3 0.13367155516426266 1103 4 0.044116284258080896 1103 5 -0.09713669289901361 1103 6 0.018811387776182187 1103 7 0.09442812796165612 1103 8 0.056360780390853904 1103 9 0.13670920178565638 1103 10-11 0.1854605272456027 1103 12-13 0.14586579524508636 1103 14-15 0.22337465106730292 1103 16-17 0.08337409595490897 1103 18-19 0.03193774257736237 1103 20-21 0.1748861893628657 1103 22-23 0.08882005587621933 1103 24-25 0.22432321031110547 1103 26-27 0.19984822627358767 1103 28-29 0.12003697142322522 1103 30-31 0.2881448385502239 1103 32-33 0.1373074712054887 1103 34-35 0.11839994247659291 1103 36-37 0.14197954697454662 1103 38-39 0.22005826842526943 1103 40-41 0.2581069840845842 1103 42-43 0.022835316167672204 1103 44-45 0.24136772059610934 1103 46-47 0.0723472963436862 1103 48-49 0.13331409923449655 1103 50-51 0.0963651280402118 1103 52-53 0.06442685723256147 1103 54-55 0.23227490663558825 1103 56-57 0.18491866742049012 1103 58-59 0.13390755430452117 1103 60-61 0.3090956610354425 1103 62-63 0.2366041073886862 1103 64-65 0.31848649913376903 1103 66-67 0.25511368704100335 1103 68-69 0.08415196228497024 1103 70-71 0.3312889060412374 1103 72-73 0.2538689783235455 1103 74-75 0.06425353892592156 1103 76-77 0.08982482655627777 1103 78-79 0.18670188519181607 1103 80-81 0.19347096006740827 1103 82-83 0.10365751023140035 1103 84-85 0.36067650267740703 1103 86-87 0.24329174321009006 1103 88-89 0.24107501586847135 1103 90-91 0.47273137399778875 1103 92-93 0.6021797722430833 1103 94-95 0.524773805990602 1103 96-97 0.6722596476625746 1103 98-99 1.1362553364491284 1103 100-101 1.5919049244828791 1104 1 0.18129350450339388 1104 2 0.1530061403105094 1104 3 0.2599584701386135 1104 4 0.06826648628202747 1104 5 -0.00600740732826921 1104 6 0.1020616418912823 1104 7 0.20463604386662837 1104 8 0.031491642353188354 1104 9 0.15896305155632007 1104 10-11 0.030188394844316235 1104 12-13 0.2412316332006199 1104 14-15 0.24332829578999338 1104 16-17 0.2888883449350388 1104 18-19 0.2864152409531755 1104 20-21 0.263854277188166 1104 22-23 0.05563389144374753 1104 24-25 0.10384582509211526 1104 26-27 0.2857098666963296 1104 28-29 0.013687605589176144 1104 30-31 0.20499359665411276 1104 32-33 0.2945408259345612 1104 34-35 0.31800369850267884 1104 36-37 0.31850857852774794 1104 38-39 0.4481638866614688 1104 40-41 0.47470279767237855 1104 42-43 0.3391261916307897 1104 44-45 0.3838760561568151 1104 46-47 0.48380062013021785 1104 48-49 0.5633436760865109 1104 50-51 0.52407401455757 1104 52-53 0.42373390826823965 1104 54-55 0.41173967486895435 1104 56-57 0.3022920439615149 1104 58-59 0.25408156218257716 1104 60-61 0.6454109042348222 1104 62-63 0.732503148091979 1104 64-65 0.4277150232371554 1104 66-67 0.7414987753130831 1104 68-69 0.7914669655138411 1104 70-71 0.7302730309195411 1104 72-73 0.6945421248440766 1104 74-75 0.3286800582025755 1104 76-77 0.6444171400865137 1104 78-79 0.6590569908081001 1104 80-81 0.664091644744218 1104 82-83 0.5313327423492069 1104 84-85 0.5427175395840926 1104 86-87 0.6579568673021505 1104 88-89 0.7085034100366023 1104 90-91 0.6878307861676092 1104 92-93 0.9751436810699019 1104 94-95 1.2748967177107247 1104 96-97 0.9368163948331656 1104 98-99 0.7490439107317037 1104 100-101 1.1915268246200306 1105 1 0.11712401955312401 1105 2 0.16057757683815765 1105 3 0.03893781936956486 1105 4 0.1490979327576838 1105 5 0.0353891938313069 1105 6 0.05950988053604789 1105 7 0.08839120751912333 1105 8 0.15366207601357473 1105 9 0.29981865749209646 1105 10-11 0.1368448254281205 1105 12-13 0.1416285934873045 1105 14-15 0.35738380109336276 1105 16-17 0.38381985152127385 1105 18-19 0.3964564925591674 1105 20-21 0.4162984540477055 1105 22-23 0.4012668481737336 1105 24-25 0.26774846131555563 1105 26-27 0.2637435785534734 1105 28-29 0.2901055335217748 1105 30-31 0.2673621647739992 1105 32-33 0.10887211013085363 1105 34-35 0.08251137748528947 1105 36-37 0.05469658829139945 1105 38-39 0.14691193498806854 1105 40-41 0.11733675496505924 1105 42-43 0.10154728449107608 1105 44-45 0.31060932457840096 1105 46-47 0.46964150569542795 1105 48-49 0.3274687022528582 1105 50-51 0.4432968212415602 1105 52-53 0.4779515392968179 1105 54-55 0.6483525876970901 1105 56-57 0.6997838195666048 1105 58-59 0.6161695502449618 1105 60-61 0.7131126059595871 1105 62-63 0.9127974402754901 1105 64-65 0.8503548187459558 1105 66-67 0.761000145565454 1105 68-69 0.7379551056771163 1105 70-71 0.6093172918954863 1105 72-73 0.7042985713677439 1105 74-75 0.7454033786111722 1105 76-77 0.8019193438210515 1105 78-79 0.5799054718156498 1105 80-81 0.7751353795125233 1105 82-83 0.8199086593236302 1105 84-85 0.8084312711068087 1105 86-87 1.1308831002892248 1105 88-89 1.3274052108574104 1105 90-91 1.38067052137378 1105 92-93 1.393954067717285 1105 94-95 1.444750877535732 1105 96-97 1.5433696003786999 1105 98-99 1.6784307828113931 1105 100-101 1.7616055829236466 1106 1 -0.02263960311854163 1106 2 0.2681381273452601 1106 3 0.1695581218723916 1106 4 0.19926218032203025 1106 5 0.17051101311297145 1106 6 0.12111478181355295 1106 7 0.1036061322188786 1106 8 -0.008028905128533381 1106 9 0.023255387669316008 1106 10-11 -0.11334792837561025 1106 12-13 -0.23663180764261682 1106 14-15 0.008361374314560521 1106 16-17 0.11405599316810111 1106 18-19 -0.020499445099709135 1106 20-21 0.03430025645008783 1106 22-23 0.05652260188880831 1106 24-25 0.2712758278665106 1106 26-27 0.17131546421876465 1106 28-29 0.059381194468116405 1106 30-31 0.09120538943570722 1106 32-33 -0.06159071223375179 1106 34-35 -0.48886869485770745 1106 36-37 -0.060983751269539255 1106 38-39 -0.03850207324731514 1106 40-41 -0.23377874805222376 1106 42-43 -0.1102759588680371 1106 44-45 -0.2053491191604948 1106 46-47 -0.12532411286191092 1106 48-49 -0.11871959829268519 1106 50-51 -0.26987429587756395 1106 52-53 -0.20170847329530517 1106 54-55 -0.26830313661390903 1106 56-57 -0.3185370507041938 1106 58-59 -0.23780218817550036 1106 60-61 -0.328538488893237 1106 62-63 -0.06631107759085353 1106 64-65 -0.10098648960065759 1106 66-67 -0.3036529031992927 1106 68-69 -0.34339710013343705 1106 70-71 -0.21704155227751443 1106 72-73 -0.3313325444270774 1106 74-75 -0.3036436263819091 1106 76-77 -0.3022737668718172 1106 78-79 -0.14808776749198316 1106 80-81 -0.22715241454224255 1106 82-83 -0.5377453220665096 1106 84-85 -0.36343553966226594 1106 86-87 -0.24987776939797968 1106 88-89 -0.06453257371397925 1106 90-91 0.027570804528277648 1106 92-93 0.10289919096187816 1106 94-95 -0.03769533938328351 1106 96-97 0.5454093677049414 1106 98-99 0.6466036787647447 1106 100-101 1.277172346444189 1107 1 -0.004629589350798824 1107 2 -0.0032721370048633958 1107 3 -0.028916469740245532 1107 4 0.029202011964962082 1107 5 0.04506499075111492 1107 6 -0.025812963754802354 1107 7 0.07177240268588747 1107 8 -0.03156626143668717 1107 9 0.0688913953106507 1107 10-11 0.0257455445724446 1107 12-13 0.12469037175721809 1107 14-15 0.11238343604548362 1107 16-17 0.035733513641062586 1107 18-19 0.05749432368801166 1107 20-21 0.1505096261353387 1107 22-23 0.20605800022428866 1107 24-25 0.009901959893902301 1107 26-27 0.09804631681820553 1107 28-29 0.02750935354505657 1107 30-31 0.10678520659631374 1107 32-33 0.2790244770607586 1107 34-35 0.15961506730815955 1107 36-37 0.19734716040255051 1107 38-39 0.06491913859961329 1107 40-41 0.06207104066775315 1107 42-43 0.11253573275364914 1107 44-45 0.07566045156233514 1107 46-47 0.2863271345715219 1107 48-49 0.31846925793685443 1107 50-51 0.34042125795239997 1107 52-53 0.33896412128049036 1107 54-55 0.2857084444847544 1107 56-57 0.4350221958336249 1107 58-59 0.5918482350991212 1107 60-61 0.6509135169568623 1107 62-63 0.6631427147306539 1107 64-65 0.6225350266609979 1107 66-67 0.6216243571634266 1107 68-69 0.6990607748707234 1107 70-71 0.7078325610371792 1107 72-73 0.6800264362837964 1107 74-75 0.8880929905652764 1107 76-77 0.7002808872434976 1107 78-79 0.7848844207211201 1107 80-81 0.8068762928623237 1107 82-83 0.7739653553158092 1107 84-85 0.6766266771820177 1107 86-87 0.8473985762913863 1107 88-89 1.053342978305686 1107 90-91 1.236616542960281 1107 92-93 1.2021305318634958 1107 94-95 1.2883648718490974 1107 96-97 1.315818017084915 1107 98-99 1.5298137058714687 1107 100-101 2.1249727237469536 1108 1 0.1631757877878357 1108 2 0.1528069853733527 1108 3 0.2542018159415633 1108 4 9.009831466855189E-4 1108 5 0.06510705293494112 1108 6 0.05213565217198379 1108 7 0.10587403401653006 1108 8 0.05085004050158659 1108 9 0.12185564245439906 1108 10-11 0.20140916078343452 1108 12-13 0.228203132514345 1108 14-15 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2316 92-93 0.6457255333109551 2316 94-95 0.6572903760467206 2316 96-97 0.8695789936361074 2316 98-99 1.1213653800970782 2316 100-101 1.5528965874929703 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 11.0 5 31.0 6 41.0 7 124.0 8 240.0 9 488.0 10 860.0 11 1587.0 12 2619.0 13 3566.0 14 4753.0 15 6909.0 16 9830.0 17 14659.0 18 12874.0 19 6980.0 20 5573.0 21 5485.0 22 5722.0 23 6193.0 24 7110.0 25 8258.0 26 10027.0 27 12280.0 28 15082.0 29 18833.0 30 23435.0 31 29570.0 32 37722.0 33 49974.0 34 68801.0 35 97829.0 36 143201.0 37 201608.0 38 157400.0 39 12999.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.344380158474024 20.059191055573823 15.015327164459306 19.58110162149285 2 14.37010458145539 23.495973761523345 40.095799340374626 22.038122316646636 3 21.19041804046078 28.115154475515936 30.60253592217202 20.091891561851263 4 11.710424553262506 17.75135344160866 39.58084015142264 30.95738185370619 5 11.663708745135711 41.18027174886432 33.5967647394044 13.559254766595568 6 27.523812431310297 38.52118695811455 18.4165750803924 15.538425530182764 7 25.661354662758985 36.71673382993447 20.593275532218016 17.028635975088534 8 24.95003459925917 34.16615785403183 20.368583872674726 20.515223674034274 9 26.72670655757724 18.52444335897749 21.469959702039322 33.27889038140595 10-11 23.325294093702933 28.06111857369642 27.21654048113323 21.397046851467415 12-13 27.812339080471464 25.68326559024011 27.297549240103642 19.206846089184783 14-15 23.994026539667033 26.53101721821956 28.71341637155534 20.761539870558067 16-17 19.39390238938413 30.382932388977085 29.798255790287786 20.424909431350997 18-19 19.106779419546545 29.69354215003867 32.62842430903245 18.571254121382342 20-21 21.09648328343567 27.843098146086508 31.412680450768203 19.647738119709608 22-23 20.1000742364811 27.87241501602012 30.65198354891085 21.37552719858793 24-25 20.426548025068193 27.952094248429933 31.822990100027525 19.79836762647435 26-27 21.604603451469185 26.829026290026803 32.49857019866606 19.06780005983795 28-29 19.79762638937228 27.403580972850634 33.48893488751707 19.309857750260015 30-31 19.782685570800187 29.191927158197885 32.27376224651526 18.75162502448667 32-33 19.467353768297567 28.86432337738131 31.645090433951694 20.023232420369432 34-35 20.21955265193145 27.561515447551592 31.856809948304637 20.362121952212316 36-37 21.120812472015306 27.634530060650462 32.20392192778931 19.04073553954492 38-39 21.828265559490372 26.63364472666585 32.79806244148655 18.740027272357228 40-41 20.256543330483982 27.7438230146131 33.13082590466887 18.868807750234055 42-43 19.356972609459376 29.216457935155844 31.78072098367396 19.645848471710824 44-45 20.180628485366547 29.124893149346686 30.414377009809908 20.28010135547686 46-47 20.172487483209185 28.82469369479383 29.33772947449831 21.66508934749868 48-49 21.04906989050352 28.41443806732609 29.877935848902997 20.65855619326739 50-51 21.92163267798266 29.393037407904917 29.54761468636789 19.137715227744536 52-53 20.530396736677623 29.80951571697588 29.94704784214996 19.713039704196536 54-55 19.30659014124639 29.234084340782353 30.549212358041274 20.91011315992999 56-57 20.89388203687874 30.415343753816092 28.23197785647413 20.458796352831033 58-59 20.780060650466073 29.349991858997843 28.92528595270078 20.94466153783531 60-61 20.567529612895346 28.560619937314286 29.99267309805837 20.879177351731997 62-63 22.347152684495462 28.34381487361094 29.50828346969512 19.800748972198477 64-65 21.623722880286564 28.82555867627305 30.01984369275858 19.53087475068181 66-67 20.17528595270078 29.142803354092887 30.17910204746204 20.50280864574429 68-69 20.25878210607726 30.178847641144625 29.57595555012822 19.986414702649896 70-71 20.36603980950055 30.282187487279682 28.39449261204054 20.95728009117922 72-73 20.46946178226144 29.010352826483942 28.305035675452352 22.21514971580227 74-75 20.741950584116907 29.83692555053527 28.56901534578907 20.852108519558758 76-77 21.389516424471854 28.49304961940815 29.445903040664305 20.671530915455694 78-79 20.480268246021087 28.215848495949853 30.20947816176171 21.09440509626735 80-81 20.04116294215818 30.339174502381244 29.135832620995643 20.483829934464932 82-83 19.971445304172132 30.136092738650756 28.680271361197057 21.212190595980058 84-85 20.28081440757522 28.69359479585113 28.647801542215017 22.377789254358625 86-87 20.78550494565881 28.88183335368584 28.93525868034355 21.3974030203118 88-89 21.648807343183947 27.638142630357798 29.65426181462938 21.058788211828876 90-91 20.14557474750353 28.172591157229682 30.243092730625655 21.438741364641132 92-93 19.382649777264437 29.417020507954117 29.829163984765955 21.37116573001549 94-95 19.345819595392193 29.78009117922416 28.781648145886756 22.092441079496886 96-97 19.196330443277567 29.00842593723287 28.353380551145847 23.441863068343714 98-99 20.126379460971602 28.343792024936004 28.363737571558467 23.16609094253393 100-101 20.59287801392888 28.93529473428167 28.707217756011573 21.76460949577788 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1094.0 1 788.0 2 541.0 3 647.0 4 577.5 5 571.5 6 1024.0 7 1887.0 8 2666.5 9 3010.5 10 2715.0 11 2292.0 12 2251.5 13 2272.0 14 2323.0 15 2376.0 16 2412.0 17 2552.5 18 2717.0 19 2856.5 20 3089.5 21 3496.0 22 3942.0 23 4497.5 24 5291.5 25 6268.0 26 7657.0 27 9406.0 28 11052.0 29 13049.0 30 15673.0 31 17856.0 32 20342.5 33 23268.0 34 26082.5 35 28266.0 36 29815.5 37 31553.5 38 31828.5 39 31273.0 40 30846.5 41 30283.0 42 30379.0 43 31138.0 44 32744.0 45 33036.0 46 33105.5 47 40624.0 48 75867.0 49 73308.5 50 38707.5 51 29779.0 52 22150.0 53 18895.0 54 16647.5 55 14361.0 56 12623.0 57 11227.5 58 9729.5 59 8376.5 60 7250.5 61 6108.5 62 4699.5 63 3096.0 64 1879.5 65 1332.5 66 941.0 67 645.5 68 458.5 69 335.5 70 288.0 71 228.5 72 184.5 73 187.0 74 149.5 75 106.0 76 88.5 77 50.0 78 30.5 79 21.5 80 10.5 81 6.0 82 4.5 83 2.5 84 2.0 85 2.0 86 1.0 87 1.5 88 2.5 89 1.0 90 0.5 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.044470224284609436 2 1.0176252696706965E-4 3 0.0 4 0.0 5 8.141002157365572E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004681076240485204 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.035250539341393E-4 22-23 9.667440061871616E-4 24-25 5.0881263483534825E-5 26-27 0.002645825701143811 28-29 0.004274026132616925 30-31 2.544063174176741E-4 32-33 1.526437904506045E-4 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.007937477103431432 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 5.0881263483534825E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.526437904506045E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.579313713518134E-4 84-85 2.544063174176741E-4 86-87 0.0 88-89 0.0 90-91 0.009362152480970408 92-93 0.0012720315870883706 94-95 0.0 96-97 0.0 98-99 4.579313713518134E-4 100-101 0.011906215655147148 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 982680.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.16853209131393 #Duplication Level Percentage of deduplicated Percentage of total 1 77.76851147608815 42.90374621062285 2 13.39540405489356 14.78009556957024 3 4.333407686132025 7.172032229913632 4 1.778184718695851 3.9239936287062434 5 0.907505221049559 2.503286545525377 6 0.4981001147768404 1.648767130005196 7 0.3017788092173783 1.1654085744551206 8 0.18745572453249948 0.8273325723657361 9 0.13284188505967331 0.6595822619086702 >10 0.6102740234479341 5.885597213118145 >50 0.04457083906481202 1.6658709267069243 >100 0.030638490251482756 3.4328060693568223 >500 0.004827883222112712 1.7899558300159502 >1k 0.005570634487053128 6.804761973595806 >5k 5.570634487053128E-4 2.212321072799431 >10k+ 3.7137563247020854E-4 2.6244421913339795 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 12985 1.3213864126673993 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 12634 1.285667765701958 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8420 0.8568404770627265 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6960 0.7082671876908048 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 6216 0.6325558676273049 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4614 0.46953229942605934 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 4269 0.43442422762242033 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 4250 0.43249073961004597 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4179 0.42526560019538406 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 4036 0.4107135588390931 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 3484 0.3545406439532706 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 3448 0.3508771929824561 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 3405 0.34650140432287213 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 2769 0.28178043717181583 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2644 0.26906012130093215 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 2598 0.26437904506044696 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 2356 0.2397525135344161 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2034 0.20698497985101966 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1839 0.18714128709244107 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1691 0.17208043310131477 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1688 0.17177514552041356 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1661 0.1690275572923027 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1603 0.16312533072821264 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1372 0.13961818699881953 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1337 0.13605649855497212 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1331 0.13544592339316971 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1143 0.1163145683233606 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1140 0.11600928074245939 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1125 0.11448284283795335 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1116 0.11356698009524972 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1093 0.11122644197500713 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1067 0.1085806162738633 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1053 0.10715594089632434 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1049 0.10674889078845606 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1032 0.10501892783001587 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.0176252696706965E-4 0.0 0.0 0.0 6 0.0 1.0176252696706965E-4 0.0 0.0 0.0 7 0.0 1.0176252696706965E-4 0.0 0.0 0.0 8 0.0 1.0176252696706965E-4 0.0 0.0 0.0 9 0.0 1.0176252696706965E-4 0.0 0.0 0.0 10-11 0.0 1.526437904506045E-4 0.0 1.0176252696706965E-4 0.0 12-13 0.0 2.035250539341393E-4 0.0 2.544063174176741E-4 0.0 14-15 0.0 2.035250539341393E-4 0.0 3.052875809012089E-4 0.0 16-17 0.0 2.035250539341393E-4 0.0 3.052875809012089E-4 0.0 18-19 0.0 2.544063174176741E-4 0.0 5.088126348353482E-4 0.0 20-21 0.0 3.561688443847438E-4 0.0 8.141002157365572E-4 0.0 22-23 0.0 4.579313713518134E-4 0.0 0.0012211503236048357 0.0 24-25 0.0 5.088126348353482E-4 0.0 0.0016282004314731144 0.0 26-27 0.0 5.088126348353482E-4 0.0 0.0026458257011438106 0.0 28-29 0.0 5.088126348353482E-4 0.0 0.006003989091057109 0.0 30-31 0.0 5.088126348353482E-4 0.0 0.012516790816949567 0.0 32-33 0.0 5.088126348353482E-4 0.0 0.019996336549029187 0.0 34-35 0.0 5.088126348353482E-4 0.0 0.02752676354459234 0.0 36-37 0.0 5.088126348353482E-4 0.0 0.036430984654210935 0.0 38-39 0.0 5.088126348353482E-4 0.0 0.04630194977001669 0.0 40-41 0.0 6.105751618024178E-4 0.0 0.06141368502462653 0.0 42-43 0.0 6.105751618024178E-4 0.0 0.0799344649326332 0.0 44-45 0.0 6.105751618024178E-4 0.0 0.09886229494850816 0.0 46-47 0.0 7.123376887694876E-4 0.0 0.11916391907843857 0.0 48-49 0.0 7.123376887694876E-4 0.0 0.14195872511906216 0.0 50-51 0.0 7.123376887694876E-4 0.0 0.17381039605975496 0.0 52-53 0.0 7.632189522530224E-4 0.0 0.2545589612081247 0.0 54-55 0.0 8.141002157365572E-4 0.0 0.36268164611063625 0.0 56-57 0.0 9.158627427036268E-4 0.0 0.4424125859893353 0.0 58-59 0.0 0.0010176252696706964 0.0 0.5759759026336142 0.0 60-61 0.0 0.0010176252696706964 0.0 0.7587414010664713 0.0 62-63 0.0 0.0010176252696706964 0.0 0.8660499857532462 0.0 64-65 0.0 0.0012211503236048357 0.0 0.9442544877274393 0.0 66-67 0.0 0.0012211503236048357 0.0 1.1622298204909025 0.0 68-69 0.0 0.0012211503236048357 0.0 1.3837159604347296 0.0 70-71 0.0 0.0012211503236048357 0.0 1.4948406398827694 0.0 72-73 0.0 0.0012211503236048357 0.0 1.7509260389954004 0.0 74-75 0.0 0.0013737941140554402 0.0 2.09854683111491 0.0 76-77 0.0 0.0014246753775389752 0.0 2.415435340090365 0.0 78-79 0.0 0.0015264379045060448 0.0 2.7512516790816948 0.0 80-81 0.0 0.0015264379045060448 0.0 3.1901534578906663 0.0 82-83 0.0 0.0015264379045060448 0.0 3.6593804697358245 0.0 84-85 0.0 0.0015264379045060448 0.0 4.2056925957585385 0.0 86-87 0.0 0.0015264379045060448 0.0 4.936347539382098 0.0 88-89 0.0 0.0016282004314731144 0.0 5.6859811942850165 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 75 3.0780202E-4 31.666853 9 ATGGGCG 130 3.037312E-7 29.229452 5 CGACCGT 35 0.008394838 27.141634 18-19 GTTCGAC 35 0.008394838 27.141634 16-17 TCGACCG 35 0.008394838 27.141634 18-19 GTATAGC 415 0.0 26.330818 1 ATAGCCC 405 0.0 24.628521 3 GAACGCA 155 1.4102152E-6 24.516272 9 GGAACGC 160 1.8590035E-6 23.75014 8 GGGAACG 205 2.6680937E-8 23.170868 7 GAGTACT 6645 0.0 22.945961 12-13 AGTACTT 6750 0.0 21.639015 12-13 CTATACG 110 0.002877823 21.589937 4 CGCTCCG 55 0.0028788364 21.589937 34-35 TATAGCC 490 0.0 21.325571 2 GTACTTT 7215 0.0 21.09919 14-15 TAGCCCT 705 0.0 20.885586 4 CATGGGC 990 0.0 19.670832 4 ACTTTTT 8125 0.0 19.028374 16-17 GTACATG 10565 0.0 18.887062 1 >>END_MODULE