##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937382_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 985323 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.797157886297185 33.0 31.0 34.0 30.0 34.0 2 31.885636486715523 33.0 31.0 34.0 30.0 34.0 3 31.918456181374026 33.0 31.0 34.0 30.0 34.0 4 35.53047680811267 37.0 35.0 37.0 33.0 37.0 5 35.53524681754105 37.0 35.0 37.0 33.0 37.0 6 35.60816402337102 37.0 35.0 37.0 33.0 37.0 7 35.57243360806558 37.0 35.0 37.0 33.0 37.0 8 35.58150576004011 37.0 35.0 37.0 33.0 37.0 9 37.18444814543048 39.0 37.0 39.0 34.0 39.0 10-11 37.07286798339225 39.0 37.0 39.0 33.0 39.0 12-13 36.95193149860502 39.0 37.0 39.0 33.0 39.0 14-15 38.03586336663206 40.0 38.0 41.0 33.0 41.0 16-17 38.020537935276046 40.0 38.0 41.0 33.0 41.0 18-19 38.09979823875014 40.0 38.0 41.0 33.5 41.0 20-21 38.0891854752198 40.0 38.0 41.0 33.0 41.0 22-23 37.95670708995933 40.0 38.0 41.0 33.0 41.0 24-25 37.79766584155652 40.0 37.5 41.0 33.0 41.0 26-27 37.570535245802645 40.0 37.0 41.0 32.5 41.0 28-29 37.64687721691263 40.0 37.0 41.0 33.0 41.0 30-31 37.49444953583749 40.0 37.0 41.0 33.0 41.0 32-33 37.284600075305256 39.0 36.0 41.0 32.0 41.0 34-35 37.16762828026952 39.0 36.0 41.0 32.0 41.0 36-37 37.102225361632684 39.0 36.0 41.0 32.0 41.0 38-39 36.961839924572956 39.0 36.0 41.0 31.0 41.0 40-41 36.74264378280016 39.0 35.5 41.0 31.0 41.0 42-43 36.52180401756581 39.0 35.0 41.0 30.0 41.0 44-45 36.27002109968 39.0 35.0 41.0 30.0 41.0 46-47 35.807309379766835 39.0 35.0 41.0 28.5 41.0 48-49 35.41643349439727 39.0 35.0 41.0 27.0 41.0 50-51 34.514021797928194 38.0 34.0 39.5 25.0 40.5 52-53 34.563276712306525 38.0 34.5 40.0 24.5 40.5 54-55 34.91554444583147 39.0 35.0 40.0 25.0 41.0 56-57 34.776058713741584 39.0 35.0 40.0 24.0 41.0 58-59 34.593437380432604 38.5 34.5 40.0 23.0 41.0 60-61 34.29771607888986 38.0 34.0 40.0 22.5 41.0 62-63 33.93614885677083 38.0 34.0 40.0 22.0 41.0 64-65 33.54189996579802 37.5 33.0 40.0 20.0 41.0 66-67 33.1804220544938 37.0 33.0 39.5 18.5 41.0 68-69 32.76756150013752 36.0 32.5 39.0 17.0 41.0 70-71 32.296238898310506 36.0 32.0 39.0 12.0 40.0 72-73 31.858079533310395 35.0 31.5 38.5 8.0 40.0 74-75 31.40986508992483 35.0 31.0 37.5 7.0 40.0 76-77 30.91982172343485 35.0 31.0 37.0 2.0 39.0 78-79 30.44212506964721 35.0 30.5 36.5 2.0 39.0 80-81 29.96418281111879 34.0 30.0 36.0 2.0 38.5 82-83 29.497737290208388 34.0 29.0 35.5 2.0 37.0 84-85 29.05011656076231 34.0 29.0 35.0 2.0 37.0 86-87 28.57217277989045 34.0 29.0 35.0 2.0 36.0 88-89 28.09684083290454 34.0 28.0 35.0 2.0 36.0 90-91 27.616310590537317 33.5 26.5 35.0 2.0 36.0 92-93 27.20939123515842 33.0 26.0 35.0 2.0 35.0 94-95 26.723795141288697 33.0 25.5 35.0 2.0 35.0 96-97 26.05755676057496 33.0 24.5 35.0 2.0 35.0 98-99 25.28318784804577 32.0 20.0 34.5 2.0 35.0 100-101 23.380724391899918 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1671403683087611 1101 2 0.03407302586218819 1101 3 0.10290423326310005 1101 4 0.07750113909657586 1101 5 -0.047530419506337296 1101 6 0.03736530529449311 1101 7 0.05176785090529279 1101 8 0.15229584377453165 1101 9 0.056137147845866764 1101 10-11 0.17143923420769624 1101 12-13 0.17131170666785778 1101 14-15 0.2653171824367391 1101 16-17 0.3051964380934109 1101 18-19 0.3383724352431656 1101 20-21 0.2978396026083203 1101 22-23 0.28323069922492294 1101 24-25 0.18408083453796564 1101 26-27 0.22413760498659485 1101 28-29 0.2626069362915828 1101 30-31 0.38913320909316695 1101 32-33 0.359579254576623 1101 34-35 0.28493575699154405 1101 36-37 0.36479308830236334 1101 38-39 0.40543877600899947 1101 40-41 0.3285511868905502 1101 42-43 0.515969937349908 1101 44-45 0.48004523384726383 1101 46-47 0.6515774644949701 1101 48-49 0.5671535527978335 1101 50-51 0.39291495187168834 1101 52-53 0.5472365541639377 1101 54-55 0.659196032584056 1101 56-57 0.518029224860868 1101 58-59 0.6039170690911888 1101 60-61 0.5653020601079888 1101 62-63 0.5073252254452285 1101 64-65 0.5524888094178735 1101 66-67 0.6478188995562348 1101 68-69 0.7565054966458007 1101 70-71 0.9552907302704696 1101 72-73 0.9044496891419875 1101 74-75 0.8266963522126396 1101 76-77 0.8096740256172623 1101 78-79 0.7375974673298025 1101 80-81 0.7293869864803924 1101 82-83 0.7096393613429015 1101 84-85 0.9468188993449935 1101 86-87 1.0939682449307 1101 88-89 0.8679738001082242 1101 90-91 0.9284402563325322 1101 92-93 1.1469015244332859 1101 94-95 1.142753962771554 1101 96-97 1.247724126895836 1101 98-99 1.1151099742054669 1101 100-101 1.4869507180532047 1102 1 -0.07106477791139909 1102 2 0.06563708752833364 1102 3 0.07723666419662578 1102 4 -0.10235944985009837 1102 5 -0.1762210528355368 1102 6 -0.1575874345677164 1102 7 -0.029714217448869817 1102 8 -0.0759639696364971 1102 9 -0.013652091223306684 1102 10-11 0.10526681953829353 1102 12-13 0.07291748999922021 1102 14-15 0.11958957961622474 1102 16-17 0.5116719664158538 1102 18-19 0.22941289075824756 1102 20-21 0.18281202983457945 1102 22-23 0.2911093820102124 1102 24-25 0.08965847256253312 1102 26-27 0.19364274507780976 1102 28-29 0.2203093745648914 1102 30-31 0.16010204565515807 1102 32-33 0.1637613619467544 1102 34-35 0.21133573486755353 1102 36-37 0.2815138192312574 1102 38-39 0.3580168191418167 1102 40-41 0.5062692614454178 1102 42-43 0.45766760999553924 1102 44-45 0.3493058888669438 1102 46-47 0.48380098558826035 1102 48-49 0.37331785035357967 1102 50-51 0.3247745482284117 1102 52-53 0.4374073723181695 1102 54-55 0.2644696151910111 1102 56-57 0.06626251937755967 1102 58-59 0.12520356433677193 1102 60-61 0.08257148101247225 1102 62-63 0.06518245692647895 1102 64-65 0.1409470361798384 1102 66-67 0.2273142931316059 1102 68-69 0.11535853204269841 1102 70-71 0.22125550827563956 1102 72-73 0.21527161043139742 1102 74-75 0.34349376968491185 1102 76-77 0.5735968188610698 1102 78-79 0.35285412807182226 1102 80-81 0.43055873593513994 1102 82-83 0.36750825276641663 1102 84-85 0.4656269094263621 1102 86-87 0.42757900018104067 1102 88-89 0.5132424795599668 1102 90-91 0.6037269339515703 1102 92-93 0.8407779619322575 1102 94-95 0.829240538141871 1102 96-97 1.0498810667290392 1102 98-99 0.9653555804253031 1102 100-101 1.2799747838750797 1103 1 0.24727420739347394 1103 2 0.10787726307192003 1103 3 0.12214822746546261 1103 4 0.0038194849195036795 1103 5 0.1770255061112067 1103 6 0.12334381086083823 1103 7 0.11161998030363662 1103 8 0.22251664742581312 1103 9 0.08427496509692389 1103 10-11 0.24758180058756096 1103 12-13 0.36138248102255943 1103 14-15 0.4274690928817364 1103 16-17 0.26250978807953373 1103 18-19 0.35360665364141397 1103 20-21 0.1519314859154406 1103 22-23 0.1323313715042005 1103 24-25 0.06888747715060362 1103 26-27 0.2776867307151534 1103 28-29 0.21449116856715733 1103 30-31 0.11915101832621389 1103 32-33 0.20949329226126423 1103 34-35 0.1254706507529093 1103 36-37 0.1810708044031415 1103 38-39 0.12348161068380392 1103 40-41 0.06916240593641021 1103 42-43 0.10391902298842126 1103 44-45 0.055355160760015565 1103 46-47 0.12825623527240992 1103 48-49 0.13423114246077006 1103 50-51 0.12639284066806766 1103 52-53 0.07256845350707408 1103 54-55 0.00777784712132501 1103 56-57 -0.04842928193901486 1103 58-59 -0.08619263886822637 1103 60-61 -0.11256283642884313 1103 62-63 -0.12032688545001236 1103 64-65 -0.008407974126697582 1103 66-67 0.020206107746936652 1103 68-69 0.08577046102733021 1103 70-71 0.19071491382121764 1103 72-73 0.2696469013416909 1103 74-75 0.20647169374901253 1103 76-77 0.23355343166777232 1103 78-79 0.11628420985917387 1103 80-81 0.19517822526288953 1103 82-83 0.037521219054067245 1103 84-85 0.24182961572332218 1103 86-87 0.24964329966903875 1103 88-89 0.3471867245230662 1103 90-91 0.3889137814813246 1103 92-93 0.4500239224349514 1103 94-95 0.5428792441728554 1103 96-97 0.6176149586101509 1103 98-99 0.7878271543331401 1103 100-101 1.1220761536470043 1104 1 -0.056422547522792854 1104 2 -0.08291906796831228 1104 3 0.07244181373465963 1104 4 -0.06267519925810916 1104 5 -0.04372183597759971 1104 6 -0.12068544353427058 1104 7 0.09965243114096722 1104 8 0.022624353631080396 1104 9 0.06957654247167255 1104 10-11 0.09788928443163769 1104 12-13 0.04416859734526213 1104 14-15 0.006039032427281654 1104 16-17 -0.010692213171246578 1104 18-19 -0.11618349297800279 1104 20-21 -0.09345017623451213 1104 22-23 -0.03918120580106432 1104 24-25 -0.0976488997516256 1104 26-27 -0.34438174958503254 1104 28-29 -0.29720016629490686 1104 30-31 -0.34268189807988136 1104 32-33 -0.23838124738156097 1104 34-35 -0.0976601254266356 1104 36-37 0.021913171160143463 1104 38-39 -0.09837058860741621 1104 40-41 -0.10858062790418899 1104 42-43 -0.08510710445234082 1104 44-45 -0.09527544169985447 1104 46-47 -0.19977256745151095 1104 48-49 -0.02565177047092959 1104 50-51 -0.21926329858563065 1104 52-53 -0.3662083861844039 1104 54-55 -0.16514359683670676 1104 56-57 -0.09433297174514621 1104 58-59 -0.08489408421601752 1104 60-61 -0.18949459933621426 1104 62-63 -0.4046278374338925 1104 64-65 -0.23717247972047772 1104 66-67 -0.040522820055166164 1104 68-69 0.2123443613817173 1104 70-71 0.01928485336676289 1104 72-73 0.2210053892855477 1104 74-75 -0.08127238240969703 1104 76-77 -0.10124175282253134 1104 78-79 -0.11770057229633935 1104 80-81 -0.23948666946298047 1104 82-83 -0.03955299399575196 1104 84-85 0.14779080674548695 1104 86-87 0.20124673237003066 1104 88-89 0.008211844389645506 1104 90-91 0.002747080070722774 1104 92-93 0.3263841475756628 1104 94-95 0.2813989787291753 1104 96-97 0.19923889023408137 1104 98-99 0.3028990742831077 1104 100-101 0.5651126000371072 1105 1 0.2688338902434779 1105 2 0.16889062247237518 1105 3 0.15445788316221254 1105 4 0.2814099052518628 1105 5 0.43550546701052184 1105 6 0.24764868758506964 1105 7 0.20803303558766117 1105 8 0.3294950334364515 1105 9 0.3399581409413912 1105 10-11 0.42108294102418853 1105 12-13 0.30534631288961833 1105 14-15 0.31841076769437393 1105 16-17 0.24325270108051456 1105 18-19 0.23261175845297544 1105 20-21 0.18653694914993935 1105 22-23 0.03919164086446614 1105 24-25 0.2567744110052814 1105 26-27 0.3820977766584619 1105 28-29 0.1758520896054918 1105 30-31 0.11067513041484034 1105 32-33 0.23464670814048816 1105 34-35 0.27095506478571707 1105 36-37 0.12592933595522737 1105 38-39 0.2525708905795412 1105 40-41 -0.014067745035674761 1105 42-43 0.23741256051421544 1105 44-45 0.1399022445006466 1105 46-47 0.2022203386719994 1105 48-49 0.5005692547666243 1105 50-51 0.26881301010436687 1105 52-53 0.29020856429234954 1105 54-55 0.4051697771745424 1105 56-57 0.4656958402506248 1105 58-59 0.5081309736005437 1105 60-61 0.3039273340935864 1105 62-63 0.22912190351490835 1105 64-65 0.5307144327376463 1105 66-67 0.4069879042061544 1105 68-69 0.4928981899292566 1105 70-71 0.7263291204031717 1105 72-73 0.5410436646739463 1105 74-75 0.4465634717440139 1105 76-77 0.6460778695954374 1105 78-79 0.639019628562334 1105 80-81 0.6943677006620383 1105 82-83 0.8169037540817676 1105 84-85 0.905774168780777 1105 86-87 0.7549028847673327 1105 88-89 0.6966287251747438 1105 90-91 0.8579292045290074 1105 92-93 0.9928880475571411 1105 94-95 1.1770040948299645 1105 96-97 1.1700261674729724 1105 98-99 1.1047382002764436 1105 100-101 1.3991520198357712 1106 1 0.0656200956546158 1106 2 0.09116351542596846 1106 3 -0.027082783206225258 1106 4 0.051630219382779785 1106 5 0.018197822495380933 1106 6 0.05430740157019187 1106 7 0.18024835444048648 1106 8 0.13568362796567612 1106 9 0.17999844281192878 1106 10-11 -0.16221104310358214 1106 12-13 -0.07092684101133528 1106 14-15 -0.13989201822937503 1106 16-17 -0.022200663897869788 1106 18-19 0.15223738182722713 1106 20-21 0.12216061334795825 1106 22-23 0.3208749998783347 1106 24-25 0.28916760897734406 1106 26-27 0.050462154443970064 1106 28-29 0.24158234873036832 1106 30-31 0.09004320288364909 1106 32-33 0.28129570908487267 1106 34-35 0.17778546556773023 1106 36-37 0.11319572593485105 1106 38-39 0.18006117753879636 1106 40-41 -0.026439445915443116 1106 42-43 -0.04523744279940445 1106 44-45 0.04863435096717694 1106 46-47 0.1300492359001595 1106 48-49 0.3070379535091021 1106 50-51 0.1433537634552593 1106 52-53 0.13430338759370386 1106 54-55 0.02491924699577197 1106 56-57 0.10245348642256147 1106 58-59 0.223149012104777 1106 60-61 0.16919720751340606 1106 62-63 0.3366332526541953 1106 64-65 0.5768399856975321 1106 66-67 0.3556149275837228 1106 68-69 0.15955812581076145 1106 70-71 0.38659532813382924 1106 72-73 0.4802361982657395 1106 74-75 0.3356344370007385 1106 76-77 0.675573205679072 1106 78-79 0.6065855281265939 1106 80-81 0.6370685265811566 1106 82-83 0.45232818703900435 1106 84-85 0.3496254412098274 1106 86-87 0.5050654591166222 1106 88-89 0.6295548977810661 1106 90-91 0.5889059346514465 1106 92-93 0.842820532604442 1106 94-95 0.9973009328106421 1106 96-97 0.9791851092692845 1106 98-99 1.3783285667625194 1106 100-101 1.468372449943299 1107 1 0.16296621519074606 1107 2 0.058900654884787684 1107 3 0.13328370504831 1107 4 0.053600186673889993 1107 5 0.216718488567345 1107 6 0.21915277772243513 1107 7 0.1751190056447669 1107 8 0.18301859674355114 1107 9 0.29587340564273745 1107 10-11 0.21154378889166026 1107 12-13 0.08418949856642399 1107 14-15 0.2148228940513235 1107 16-17 0.3159089108865487 1107 18-19 0.32613345676032424 1107 20-21 0.23151238260160056 1107 22-23 0.2376067126348005 1107 24-25 0.2472500502500239 1107 26-27 0.37746743246953685 1107 28-29 0.3048907787036086 1107 30-31 0.35899019961276224 1107 32-33 0.22461268797277256 1107 34-35 0.02537318486597684 1107 36-37 0.023236983740375194 1107 38-39 0.14932225395865828 1107 40-41 0.13584125583894036 1107 42-43 0.24314904248159763 1107 44-45 -0.07607758777798779 1107 46-47 -0.0034810941622893665 1107 48-49 -0.05022304857237003 1107 50-51 -0.11936739325005874 1107 52-53 -0.05882774532333457 1107 54-55 0.02421303230650551 1107 56-57 -0.07770559772848884 1107 58-59 0.008227439104487644 1107 60-61 0.08084603296686765 1107 62-63 0.10743870909190179 1107 64-65 0.0641188289922141 1107 66-67 0.18485035725960586 1107 68-69 0.17911841424371033 1107 70-71 0.17105117113116108 1107 72-73 0.07642078048399625 1107 74-75 -0.049364076231494636 1107 76-77 -0.06984569113924266 1107 78-79 0.01306783559016722 1107 80-81 0.1647688685377453 1107 82-83 0.23605923074997648 1107 84-85 0.2512107073412544 1107 86-87 0.2462563600978882 1107 88-89 -0.0038732169974018404 1107 90-91 0.2986238204676823 1107 92-93 0.5842344487507383 1107 94-95 0.46628324027421897 1107 96-97 0.6884848416510856 1107 98-99 0.6099762771401558 1107 100-101 0.8603680639783882 1108 1 0.019406095379018495 1108 2 -0.088938455085259 1108 3 0.010449943815959983 1108 4 0.10016094833413547 1108 5 0.07580599391608445 1108 6 0.02338897797917383 1108 7 0.01444292519975221 1108 8 0.11971628608886675 1108 9 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-1.5461346798787048 2316 64-65 -1.333028828273676 2316 66-67 -1.465987132881299 2316 68-69 -1.5392565404577496 2316 70-71 -1.8018056748111562 2316 72-73 -1.358058872475862 2316 74-75 -1.8098833307773354 2316 76-77 -2.1013848024351027 2316 78-79 -2.4540107813653442 2316 80-81 -2.4791112981608983 2316 82-83 -2.0689625125511704 2316 84-85 -2.2210292790158874 2316 86-87 -2.4689663488616347 2316 88-89 -2.5457777010203415 2316 90-91 -2.9679254696774855 2316 92-93 -2.6175945465808432 2316 94-95 -2.4188163365840865 2316 96-97 -2.8920313219352813 2316 98-99 -2.869078475476499 2316 100-101 -3.3479246338963087 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3282.0 3 629.0 4 512.0 5 532.0 6 657.0 7 758.0 8 905.0 9 1124.0 10 1475.0 11 2135.0 12 3323.0 13 4825.0 14 5434.0 15 7403.0 16 9711.0 17 13525.0 18 11062.0 19 6670.0 20 6126.0 21 6325.0 22 6722.0 23 7528.0 24 8240.0 25 9292.0 26 10876.0 27 12705.0 28 15116.0 29 18286.0 30 22707.0 31 28455.0 32 36427.0 33 47503.0 34 64904.0 35 91415.0 36 130477.0 37 186252.0 38 174573.0 39 27420.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.76137124801241 19.078919240267687 14.66112754569164 20.49858196602826 2 13.834744986629655 21.827748450301797 41.399531122172796 22.93797544089575 3 20.36459319043462 27.606192141995166 30.94890689198347 21.080307775586746 4 11.213554396468743 16.741726921769782 40.62136866761772 31.423350014143754 5 11.865772713535778 39.69917100058916 34.60856310499811 13.826493180876948 6 27.490481846359106 37.38244673494789 19.712543624870854 15.414527793822149 7 25.465567573008823 35.93230955070632 21.197648750301227 17.404474125983626 8 24.36040576102563 33.08400343286193 21.53287046640554 21.022720339706904 9 26.36636593889393 17.973407300231926 22.200033960242113 33.46019280063203 10-11 22.99655696166486 26.83454550724431 28.36715775685366 21.801739774237163 12-13 27.36343501685008 24.887022081030896 27.845599555816786 19.903943346302235 14-15 23.77617862270651 25.494134849649598 29.516562338576176 21.213124189067717 16-17 19.53090999312614 29.301906783969674 30.480326121882868 20.686857101021317 18-19 18.93582244727283 29.18902979971682 33.09636927957539 18.77877847343496 20-21 20.922605778474363 27.30797601902129 31.450200527793015 20.319217674711332 22-23 19.72654998896805 27.404431285186583 30.796272929425005 22.072745796420364 24-25 20.206811396688597 27.082563416180232 32.29605688889251 20.414568298238667 26-27 21.372468781533 25.99867323103826 32.779021800186555 19.849836187242175 28-29 19.466524585513316 26.695784490818532 33.999677673719376 19.83801324994878 30-31 19.679279464337014 28.3382966596314 32.83315342652557 19.149270449506016 32-33 19.039171961892677 28.21203884758799 31.9931810301261 20.755608160393237 34-35 19.978715593344784 26.80396278432174 32.18206354000462 21.03525808232886 36-37 20.93601255459209 26.822019745929136 32.64016015543781 19.601807544040966 38-39 21.63158135538938 26.02798013329199 32.892924137440204 19.44751437387843 40-41 20.057535714539892 26.906309001050268 33.70780519397334 19.328350090436505 42-43 19.191925354058828 28.45129193725007 32.26869122531959 20.08809148337151 44-45 19.833074222336556 28.683943218595797 30.895554712752837 20.587427846314814 46-47 19.69403017703767 28.061198031852747 29.826694039328373 22.418077751781208 48-49 20.354624821602076 27.751929392300532 30.518590754271763 21.37485503182563 50-51 21.567647435675802 28.37784656561363 30.356638037458637 19.69786796125193 52-53 20.08916697984106 28.6410401017482 30.95524208168843 20.31455083672231 54-55 19.233798331214004 28.121092993305435 31.600315026446257 21.044793649034304 56-57 20.499004167348687 29.59508905560091 29.158427367250273 20.747479409800132 58-59 20.1414729864373 28.396876715671322 29.966804937066634 21.494845360824744 60-61 20.24711214495294 27.367929304739114 30.995107777332702 21.389850772975247 62-63 22.10187365535641 27.254375048295326 30.20110174351216 20.442649552836105 64-65 21.243227801064847 27.84152774911373 31.097622346909414 19.817622102912008 66-67 19.906743057451177 28.171668819414684 31.146985161542517 20.774602961591622 68-69 19.696774075489806 29.46812706881267 30.622138227185115 20.21296062851241 70-71 19.779875652620397 29.213703630272438 29.399512981236054 21.60690773587112 72-73 19.826965221727814 28.282071394765644 29.239207185737108 22.65175619776943 74-75 20.250697355919176 28.54942343268399 29.924868065394623 21.275011146002207 76-77 20.951006916802147 27.385930718104984 30.40825705329791 21.254805311794964 78-79 20.083023916906946 27.31015116331087 31.35794837591961 21.248876543862576 80-81 19.768002834639745 29.15540851398485 30.319207073585236 20.75738157779017 82-83 19.419501700918882 29.030710075173904 29.863682484297254 21.686105739609957 84-85 19.95626419799651 27.53037589712022 29.78212921784384 22.73123068703943 86-87 20.560646001478165 27.87904449850608 30.144837955738367 21.41547154427739 88-89 21.563755677153885 26.41493609310151 30.531307899345045 21.490000330399557 90-91 19.804404085157778 26.980676782808626 31.61686624620848 21.598052885825116 92-93 19.428621389742595 28.34445380893657 31.073706234413457 21.153218566907377 94-95 19.241406618922696 28.716834604195906 30.158760818767256 21.882997958114146 96-97 18.841411406862658 27.943743385745844 29.522828262701655 23.69201694468984 98-99 19.75535637671641 27.495135187892565 29.630721618879335 23.11878681651169 100-101 20.726054120873815 27.315446885035588 30.563685399929042 21.39481359416156 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1766.0 1 1103.0 2 517.0 3 633.0 4 562.5 5 546.5 6 993.5 7 2002.5 8 3067.0 9 3333.0 10 2766.5 11 2217.5 12 2092.5 13 2149.5 14 2255.5 15 2316.5 16 2342.5 17 2420.5 18 2557.0 19 2793.0 20 3054.5 21 3442.0 22 3909.0 23 4409.5 24 5098.5 25 6094.5 26 7634.5 27 9490.5 28 11173.5 29 13085.0 30 15285.0 31 17467.0 32 20066.5 33 23166.5 34 26332.5 35 28628.5 36 30198.0 37 31465.5 38 31777.5 39 31245.0 40 30671.0 41 30294.0 42 30467.5 43 31230.0 44 32722.0 45 33021.0 46 33713.5 47 41914.5 48 75353.0 49 72193.5 50 38447.5 51 29526.5 52 22223.5 53 19004.0 54 16840.0 55 14841.5 56 13110.5 57 11475.0 58 9943.0 59 8568.0 60 7508.0 61 6291.0 62 4911.5 63 3260.5 64 1967.0 65 1382.5 66 939.0 67 648.0 68 472.5 69 355.5 70 282.5 71 225.0 72 201.0 73 190.5 74 145.5 75 109.0 76 88.5 77 57.5 78 36.0 79 24.5 80 19.0 81 12.5 82 9.5 83 6.0 84 2.5 85 4.0 86 6.0 87 5.5 88 4.5 89 2.5 90 1.5 91 1.0 92 1.0 93 1.0 94 1.0 95 2.5 96 4.0 97 4.0 98 2.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30213442698485676 2 0.2588998734425158 3 0.2566671030717846 4 0.25950881081635163 5 0.2610311542509411 6 0.19678826131126545 7 0.18734973201681074 8 0.1915108040713553 9 0.18471100339685567 10-11 0.20648051451148505 12-13 0.19739719868510125 14-15 0.1915108040713553 16-17 0.1915108040713553 18-19 0.18679153942412793 20-21 0.18202152999574758 22-23 0.1843050451476318 24-25 0.17471428150971813 26-27 0.1756276875704718 28-29 0.18750196636026967 30-31 0.1972449643416423 32-33 0.19181527275827318 34-35 0.20034039599197423 36-37 0.18049918656115813 38-39 0.17943354615694548 40-41 0.17862162965849776 42-43 0.18268121215073635 44-45 0.18592887814452722 46-47 0.18877058588909423 48-49 0.1946062357216872 50-51 0.19739719868510125 52-53 0.21429521080904435 54-55 0.19420027747246332 56-57 0.17496800541548305 58-59 0.17689630709929638 60-61 0.17466353672856513 62-63 0.18176780608998266 64-65 0.17314119329397568 66-67 0.17217704245206902 68-69 0.16918310036404305 70-71 0.18146333740306478 72-73 0.17897684312656864 74-75 0.18131110305960582 76-77 0.1802454626553932 78-79 0.17770822359774407 80-81 0.1802962074365462 82-83 0.17628736972546058 84-85 0.17146661551592726 86-87 0.1696905481755729 88-89 0.16903086602058412 90-91 0.17390236501127043 92-93 0.17273523504475183 94-95 0.16984278251903184 96-97 0.1676607569294536 98-99 0.17547545322701286 100-101 0.16603692393255814 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 985323.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.6843019932537 #Duplication Level Percentage of deduplicated Percentage of total 1 78.71721155054747 44.62030191598071 2 13.001791340345866 14.739949335788715 3 4.074098107353024 6.928122224020263 4 1.6923529701219253 3.837193873502842 5 0.8703962021359609 2.466890058782795 6 0.4329466764844579 1.4724768094092309 7 0.2653019243976316 1.0526918081362695 8 0.18170958866253342 0.824006495905356 9 0.1111437700764912 0.5670096324916123 >10 0.57737505672898 5.826516115659841 >50 0.03721289900783252 1.4701415250861125 >100 0.02790579839663027 3.2241764223016416 >500 0.004650966258814831 1.8227441095877983 >1k 0.004829849576461556 6.015380037671704 >5k 7.155332705868971E-4 2.859140835449886 >10k+ 3.577666352934486E-4 2.2732588002252365 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 11798 1.197373856085771 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 10621 1.0779206412516504 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8497 0.8623568109137815 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 8238 0.8360710142765367 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6058 0.6148237684495338 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 5404 0.548449594701433 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 4924 0.49973460479456994 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 4593 0.4661415596712956 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4192 0.42544424518660373 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 3972 0.4031165414792916 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 3313 0.33623491991966087 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2956 0.3000031461764315 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2619 0.26580116367932144 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2287 0.23210662899374115 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2170 0.22023235020394324 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2075 0.21059084178487666 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 2045 0.2075461549156977 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2018 0.20480593673343667 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1978 0.20074635424119805 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1969 0.19983294818044436 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1816 0.1843050451476318 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1740 0.1765918384123785 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1708 0.1733441724185876 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1519 0.1541626451427603 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1460 0.14817476096670837 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1433 0.14543454278444734 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1426 0.14472411584830558 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1297 0.13163196231083613 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1293 0.13122600406161228 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1254 0.12726791113167965 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1164 0.11813385052414284 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1080 0.10960872729044181 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1023 0.10382382223900183 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 5.074478115298232E-5 0.0 1.0148956230596464E-4 0.0 12-13 0.0 1.0148956230596464E-4 0.0 2.0297912461192928E-4 0.0 14-15 0.0 1.0148956230596464E-4 0.0 3.552134680708763E-4 0.0 16-17 0.0 1.0148956230596464E-4 0.0 4.0595824922385856E-4 0.0 18-19 0.0 1.0148956230596464E-4 0.0 4.0595824922385856E-4 0.0 20-21 0.0 1.0148956230596464E-4 0.0 4.567030303768409E-4 0.0 22-23 0.0 1.0148956230596464E-4 0.0 0.0011671299665185933 0.0 24-25 0.0 1.0148956230596464E-4 0.0 0.0019790464649663106 0.0 26-27 0.0 1.0148956230596464E-4 0.0 0.002537239057649116 0.0 28-29 0.0 1.0148956230596464E-4 0.0 0.004871498990686303 0.0 30-31 0.0 1.0148956230596464E-4 0.0 0.009895232324831553 0.0 32-33 0.0 1.5223434345894694E-4 0.0 0.01963823030620416 0.0 34-35 0.0 2.0297912461192928E-4 0.0 0.028975270038352905 0.0 36-37 0.0 2.0297912461192928E-4 0.0 0.03790635152127779 0.0 38-39 0.0 2.0297912461192928E-4 0.0 0.045416579131919176 0.0 40-41 0.0 2.0297912461192928E-4 0.0 0.0576968161709409 0.0 42-43 0.0 2.0297912461192928E-4 0.0 0.07576195826140261 0.0 44-45 0.0 2.0297912461192928E-4 0.0 0.09169581954343906 0.0 46-47 0.0 2.0297912461192928E-4 0.0 0.10813712863700534 0.0 48-49 0.0 2.0297912461192928E-4 0.0 0.12762312459975053 0.0 50-51 0.0 2.0297912461192928E-4 0.0 0.1547208377354431 0.0 52-53 0.0 2.0297912461192928E-4 0.0 0.22865598387533834 0.0 54-55 0.0 2.0297912461192928E-4 0.0 0.3240054276617921 0.0 56-57 0.0 3.0446868691789394E-4 0.0 0.38971991925490423 0.0 58-59 0.0 3.0446868691789394E-4 0.0 0.5115581388032148 0.0 60-61 0.0 3.0446868691789394E-4 0.0 0.6848008216594964 0.0 62-63 0.0 3.0446868691789394E-4 0.0 0.780454734132868 0.0 64-65 0.0 3.0446868691789394E-4 0.0 0.8549480728654462 0.0 66-67 0.0 3.0446868691789394E-4 0.0 1.0734043557290351 0.0 68-69 0.0 3.0446868691789394E-4 0.0 1.2647121806757784 0.0 70-71 0.0 3.0446868691789394E-4 0.0 1.3588944944957135 0.0 72-73 0.0 3.0446868691789394E-4 0.0 1.5910011234894545 0.0 74-75 0.0 3.0446868691789394E-4 0.0 1.917391555865437 0.0 76-77 0.0 3.0446868691789394E-4 0.0 2.2128276717381 0.0 78-79 0.0 3.0446868691789394E-4 0.0 2.5102935788568823 0.0 80-81 0.0 3.0446868691789394E-4 0.0 2.9229501391929347 0.0 82-83 0.0 3.0446868691789394E-4 0.0 3.3540270550875197 0.0 84-85 0.0 3.552134680708763E-4 0.0 3.8545228315993842 0.0 86-87 0.0 4.0595824922385856E-4 0.0 4.571445099728718 0.0 88-89 0.0 5.074478115298232E-4 0.0 5.269287330144531 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 50 0.0016118351 38.01854 2 CGGGAGT 285 0.0 28.348598 4 GTGTAGC 570 0.0 27.524622 1 CGCGGAA 35 0.008399058 27.13884 82-83 GTCGGGA 295 0.0 25.775284 2 TAGCCCT 800 0.0 24.356867 4 TACCTGG 755 0.0 23.289503 2 GTAGCCC 430 0.0 23.207813 3 GAGTACT 5700 0.0 22.54005 12-13 AGTACTT 5705 0.0 22.187279 12-13 GGTCGGG 355 0.0 21.427616 1 ATGGGAT 2185 0.0 20.87975 5 GTACTTT 6185 0.0 20.849354 14-15 GTACATG 9500 0.0 20.718533 1 TACATGG 9485 0.0 20.692749 2 GTATAGC 530 0.0 20.631662 1 GTATTAG 305 2.055458E-10 20.264027 1 ATAGCCC 470 0.0 20.2216 3 ACATGGG 9445 0.0 20.02463 3 TACTTTT 6695 0.0 19.119242 14-15 >>END_MODULE