##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937382_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 985323 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11974448987794 31.0 31.0 34.0 31.0 34.0 2 32.309059059820996 33.0 31.0 34.0 31.0 34.0 3 32.399375636212696 34.0 31.0 34.0 31.0 34.0 4 35.9956603063158 37.0 35.0 37.0 35.0 37.0 5 35.87865603462012 37.0 35.0 37.0 35.0 37.0 6 36.003599834774995 37.0 35.0 37.0 35.0 37.0 7 35.97195640414362 37.0 35.0 37.0 35.0 37.0 8 35.97014177076959 37.0 35.0 37.0 35.0 37.0 9 37.63430671972541 39.0 37.0 39.0 35.0 39.0 10-11 37.563196028104485 39.0 37.0 39.0 35.0 39.0 12-13 37.48182271194319 39.0 37.0 39.0 35.0 39.0 14-15 38.5620918216666 40.0 38.0 41.0 34.0 41.0 16-17 38.56566526915539 40.0 38.0 41.0 34.0 41.0 18-19 38.686010577242186 40.0 38.0 41.0 34.0 41.0 20-21 38.647613523687156 40.0 38.0 41.0 34.0 41.0 22-23 38.53895829083458 40.0 38.0 41.0 34.0 41.0 24-25 38.383855852344865 40.0 38.0 41.0 34.0 41.0 26-27 38.08151083451823 40.0 37.5 41.0 33.5 41.0 28-29 38.05908164124861 40.0 37.0 41.0 33.5 41.0 30-31 38.15877737554081 40.0 37.0 41.0 34.0 41.0 32-33 38.07450298024099 40.0 37.0 41.0 34.0 41.0 34-35 37.95773162709081 40.0 37.0 41.0 33.5 41.0 36-37 37.76306297528831 40.0 37.0 41.0 33.0 41.0 38-39 37.55970884674366 40.0 36.5 41.0 33.0 41.0 40-41 37.35073930071662 40.0 36.0 41.0 33.0 41.0 42-43 37.15446457659062 40.0 36.0 41.0 32.5 41.0 44-45 36.824076977803216 40.0 36.0 41.0 31.5 41.0 46-47 36.358512386293626 40.0 36.0 41.0 30.5 41.0 48-49 35.95366037329891 39.0 35.5 41.0 29.5 41.0 50-51 35.709173540047274 39.0 35.0 41.0 28.0 41.0 52-53 35.404278089519885 39.0 35.0 41.0 27.0 41.0 54-55 35.21722064744252 39.0 35.0 40.0 27.0 41.0 56-57 35.06192842347129 39.0 35.0 40.0 26.0 41.0 58-59 34.86310884857047 38.5 35.0 40.0 26.0 41.0 60-61 34.521205736596016 38.0 34.0 40.0 25.0 41.0 62-63 34.116860156517205 38.0 34.0 40.0 23.5 41.0 64-65 33.7282627118214 37.0 33.5 40.0 22.0 41.0 66-67 33.354823748151624 37.0 33.0 39.5 22.0 41.0 68-69 32.97567193701964 36.0 33.0 39.0 21.0 40.0 70-71 32.5917602654155 36.0 32.5 39.0 19.5 40.0 72-73 32.12040873906323 35.0 32.0 38.0 18.0 40.0 74-75 31.68678900218507 35.0 31.5 37.0 15.5 39.5 76-77 30.30004983137509 34.0 29.5 36.0 12.5 38.5 78-79 30.856107083666977 34.5 31.0 36.0 9.5 39.0 80-81 30.719165187456298 34.5 31.0 36.0 7.0 38.0 82-83 30.40070108989641 34.0 31.0 35.5 4.5 37.0 84-85 30.068288774341003 34.0 31.0 35.0 2.0 37.0 86-87 29.69994052711649 34.0 30.0 35.0 2.0 36.0 88-89 29.258671521927326 34.0 29.5 35.0 2.0 36.0 90-91 28.763169031880913 34.0 29.0 35.0 2.0 35.5 92-93 28.430961217793556 34.0 29.0 35.0 2.0 35.0 94-95 28.089349380862927 34.0 29.0 35.0 2.0 35.0 96-97 27.63991655528187 34.0 28.5 35.0 2.0 35.0 98-99 27.024777154293567 33.0 26.5 35.0 2.0 35.0 100-101 25.492155871729373 32.0 22.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2237344887019148 1101 2 0.20797471099578502 1101 3 0.2722781017197917 1101 4 0.1721342827936354 1101 5 0.013517351457643656 1101 6 0.08298111918923468 1101 7 0.09907039808762619 1101 8 0.15068592612626475 1101 9 0.1695192794138407 1101 10-11 0.06216758739582673 1101 12-13 0.15978948957536687 1101 14-15 0.24353887798442742 1101 16-17 0.2564556725225273 1101 18-19 0.2081563228265466 1101 20-21 0.14344685967078874 1101 22-23 0.15436161164981144 1101 24-25 0.29809738389317175 1101 26-27 0.22960427738875921 1101 28-29 0.2941388202977393 1101 30-31 0.2252209349289913 1101 32-33 0.18482600629964452 1101 34-35 0.16947130073266692 1101 36-37 0.3168452428634012 1101 38-39 0.20842362102857237 1101 40-41 0.2573486281296411 1101 42-43 0.05379439312186918 1101 44-45 0.07861188712588074 1101 46-47 0.12263175622499745 1101 48-49 0.08217182567860704 1101 50-51 -0.11773369596282635 1101 52-53 0.06691115191247832 1101 54-55 0.021544647475323586 1101 56-57 0.09873035316039136 1101 58-59 0.07678158868547058 1101 60-61 0.05636827815862233 1101 62-63 0.032804649081263904 1101 64-65 0.10977929733501668 1101 66-67 0.15089991694794236 1101 68-69 -0.03578858306176613 1101 70-71 -0.1503731745899799 1101 72-73 -0.17224987854079643 1101 74-75 -0.22798094015186976 1101 76-77 -0.13393203828053757 1101 78-79 -0.05679744909933149 1101 80-81 -0.23909834526859086 1101 82-83 -0.03821902729626814 1101 84-85 -0.001661124815882431 1101 86-87 -0.17693900341308932 1101 88-89 -0.17376462001085002 1101 90-91 0.04592406725683773 1101 92-93 -0.06505136330262928 1101 94-95 -0.2759312523484212 1101 96-97 -0.26564751450709423 1101 98-99 -0.2543693699420473 1101 100-101 -0.10731766994100056 1102 1 0.14803605068572523 1102 2 0.1715867219322078 1102 3 0.08364714337717771 1102 4 0.06983548032950182 1102 5 0.014092874249094223 1102 6 0.09857778683753082 1102 7 0.1415193148341416 1102 8 0.1900374838497001 1102 9 0.14696626031096827 1102 10-11 0.2442932696145661 1102 12-13 0.142892663620934 1102 14-15 0.1712494782173266 1102 16-17 0.23137222171776983 1102 18-19 0.25114974392834455 1102 20-21 0.19834052249216683 1102 22-23 0.2985950975091427 1102 24-25 0.2655366213927479 1102 26-27 0.41733271135387895 1102 28-29 0.2476661023203448 1102 30-31 0.2307534280488781 1102 32-33 0.10960480374103554 1102 34-35 0.29162302348913016 1102 36-37 0.23405068357184433 1102 38-39 0.2539250508663571 1102 40-41 0.28389966205259753 1102 42-43 0.10464519519136672 1102 44-45 0.33299370837895736 1102 46-47 0.28461675675567477 1102 48-49 0.20829390826389016 1102 50-51 0.11607355921202611 1102 52-53 0.2585508985628593 1102 54-55 0.31474398614731314 1102 56-57 0.08297859333128343 1102 58-59 0.10889837646106315 1102 60-61 0.04154893999488962 1102 62-63 0.1866332957655743 1102 64-65 0.16606318404222975 1102 66-67 -0.04134338120126557 1102 68-69 -0.02655667428436459 1102 70-71 -0.019370359760323197 1102 72-73 0.07449953883580207 1102 74-75 -0.15976317727622913 1102 76-77 -0.04212008325203698 1102 78-79 0.11414253657986606 1102 80-81 0.06443798068360351 1102 82-83 0.029201599141604362 1102 84-85 0.24094826562326332 1102 86-87 0.21582241473486263 1102 88-89 0.3386775100513191 1102 90-91 0.09802009136601342 1102 92-93 -0.013874307079287718 1102 94-95 0.017838377089645974 1102 96-97 0.09902913885029818 1102 98-99 0.2506253457123222 1102 100-101 0.10785871658309532 1103 1 0.11399954559895065 1103 2 0.07320584554415177 1103 3 0.05384130522304531 1103 4 0.18892584538036772 1103 5 -0.03871150104181709 1103 6 -0.011587450212807937 1103 7 0.06513956884039374 1103 8 0.11484465187949411 1103 9 -0.0041824197874404945 1103 10-11 0.08925304087595265 1103 12-13 0.13722573097913227 1103 14-15 0.2621672586548627 1103 16-17 -0.004975773494678037 1103 18-19 0.17801323219219256 1103 20-21 0.10723822180727893 1103 22-23 0.0803866062530787 1103 24-25 0.11670109376458271 1103 26-27 0.01919204375629846 1103 28-29 0.09289101754077222 1103 30-31 0.1027512337540415 1103 32-33 0.2838110342084832 1103 34-35 0.22153940755722346 1103 36-37 0.241140583166235 1103 38-39 0.26302472504682584 1103 40-41 0.32749464842137854 1103 42-43 0.3878555150264589 1103 44-45 0.37033329212788146 1103 46-47 0.20095344802503945 1103 48-49 0.22569034419712608 1103 50-51 0.2659488833692194 1103 52-53 0.30636824014142405 1103 54-55 0.3410796800099405 1103 56-57 0.5493078040500805 1103 58-59 0.5185083904247563 1103 60-61 0.29039277934590757 1103 62-63 0.5514048896215513 1103 64-65 0.3244156343381306 1103 66-67 0.3091295558291236 1103 68-69 0.4061059030146552 1103 70-71 0.37432769197183546 1103 72-73 0.37403408235598334 1103 74-75 0.3565959556448455 1103 76-77 0.23558372019234142 1103 78-79 0.4940240114119696 1103 80-81 0.41464660492723837 1103 82-83 0.14936500761291427 1103 84-85 0.29531940067213824 1103 86-87 0.42912615833483514 1103 88-89 0.4891570964599907 1103 90-91 0.550103840285491 1103 92-93 0.5412689870227148 1103 94-95 0.3518665704535664 1103 96-97 0.3734672663853722 1103 98-99 0.19318552258578237 1103 100-101 0.33757894399183286 1104 1 -0.055872249272844954 1104 2 0.06486382622652087 1104 3 0.07831840896844255 1104 4 0.04664942261614158 1104 5 -0.07660845052413379 1104 6 -0.045638245848969916 1104 7 -0.047078136669995274 1104 8 -0.017500216440986094 1104 9 -0.029988479130082624 1104 10-11 0.14128903559487327 1104 12-13 0.07036276106738626 1104 14-15 0.15188565904399098 1104 16-17 -0.06145638637773487 1104 18-19 -0.023720490273234418 1104 20-21 -0.028034691819286195 1104 22-23 -0.03180243550913531 1104 24-25 -0.1096877951243087 1104 26-27 0.04500592048481167 1104 28-29 -0.09670925346193826 1104 30-31 -0.08068111980026771 1104 32-33 0.025890285129200663 1104 34-35 -0.05423103924981376 1104 36-37 0.05872622697138752 1104 38-39 -0.03981385462102338 1104 40-41 0.2171565899125838 1104 42-43 0.1282800859388331 1104 44-45 0.3481875065674629 1104 46-47 0.2643897971321181 1104 48-49 -0.035088962313885474 1104 50-51 -0.02948055169436259 1104 52-53 -0.0578090234260884 1104 54-55 0.0184257643682173 1104 56-57 -0.06492157542319887 1104 58-59 -0.04775698376744941 1104 60-61 0.029134875100197632 1104 62-63 0.10197816458506992 1104 64-65 0.12486991131679304 1104 66-67 0.19658612590764335 1104 68-69 0.2690019838985407 1104 70-71 0.14094050553587323 1104 72-73 0.25082860671148666 1104 74-75 0.11250416216280357 1104 76-77 0.31827515928621963 1104 78-79 0.3715108087158363 1104 80-81 0.27701978955651896 1104 82-83 0.32023655927714856 1104 84-85 0.32324049719079184 1104 86-87 0.18849833538994076 1104 88-89 0.2111491400983745 1104 90-91 0.34669632864627076 1104 92-93 0.46440150039913064 1104 94-95 0.40066095583331673 1104 96-97 0.361082789976507 1104 98-99 0.378738124906679 1104 100-101 0.5467643723845761 1105 1 0.07282163748555348 1105 2 0.036836778529924175 1105 3 0.040943510067187106 1105 4 0.09361792749180609 1105 5 -0.06487366026848917 1105 6 0.08302262907469071 1105 7 0.09009612363566077 1105 8 0.07940694144178195 1105 9 0.05893531671739538 1105 10-11 0.02720242721243693 1105 12-13 -5.851737672557533E-4 1105 14-15 0.08971016079453165 1105 16-17 0.16627300460893224 1105 18-19 0.10974452360090226 1105 20-21 0.10472790357115969 1105 22-23 0.07083273419920744 1105 24-25 0.07286352452378964 1105 26-27 0.03008457118107799 1105 28-29 -0.029612240849580473 1105 30-31 -0.08239865220229348 1105 32-33 0.034105647003102035 1105 34-35 0.09520482517424966 1105 36-37 0.020859818530631458 1105 38-39 0.08706621521734803 1105 40-41 -0.12005884348695162 1105 42-43 -0.1393629057403487 1105 44-45 -0.10698288034958381 1105 46-47 -0.0957216361500457 1105 48-49 -0.2233022701180687 1105 50-51 -0.32910650926682194 1105 52-53 -0.22603916726598783 1105 54-55 -0.018897782790105566 1105 56-57 -0.019104816781890577 1105 58-59 0.02612487787995832 1105 60-61 -0.011384401060304583 1105 62-63 0.050736919598740826 1105 64-65 0.24151539538949862 1105 66-67 0.046920095635790915 1105 68-69 0.004122357885954386 1105 70-71 -0.19480177473827354 1105 72-73 0.09703288761286188 1105 74-75 0.20089105719801026 1105 76-77 -0.05316804476910164 1105 78-79 -0.15414993232649365 1105 80-81 -0.20583102079232773 1105 82-83 -0.20048157650786536 1105 84-85 -0.26441096752597204 1105 86-87 -0.055165684827976236 1105 88-89 0.043701193342798206 1105 90-91 -0.14689708292511128 1105 92-93 -0.17260632523969477 1105 94-95 -0.27668779035886004 1105 96-97 -0.43473030068316376 1105 98-99 -0.30297093445776824 1105 100-101 -0.15871870996711124 1106 1 -0.15789310976828475 1106 2 -0.07762591842320177 1106 3 -0.10168286301298224 1106 4 0.03073375498488673 1106 5 -0.1099999505082323 1106 6 -0.18732505222159546 1106 7 -0.09624618131654472 1106 8 -0.06954015665785818 1106 9 -0.24625712160790414 1106 10-11 -0.32002583545800434 1106 12-13 -0.42448173920291055 1106 14-15 -0.3367420319917258 1106 16-17 -0.19000402208224898 1106 18-19 -0.27897436981659496 1106 20-21 -0.34980823142561235 1106 22-23 -0.2992077126420938 1106 24-25 0.06457067945196115 1106 26-27 -0.011649921089905035 1106 28-29 -0.11969137417298015 1106 30-31 -0.022648954569874036 1106 32-33 -0.12321972536464898 1106 34-35 -0.0812069829010369 1106 36-37 0.008187821082117352 1106 38-39 -0.18342850784708276 1106 40-41 -0.04405274643109891 1106 42-43 -0.43419407693838963 1106 44-45 -0.26750019814205217 1106 46-47 -0.1748979330170144 1106 48-49 -0.20331546245698462 1106 50-51 -0.12934301870234322 1106 52-53 -0.09662532545807778 1106 54-55 -0.12676793455377577 1106 56-57 -0.4814517690823763 1106 58-59 -0.4357053572211953 1106 60-61 -0.2929248603276662 1106 62-63 -0.24899529870236137 1106 64-65 -0.2988698333329296 1106 66-67 -0.19329903801896364 1106 68-69 -0.1553936261755524 1106 70-71 -0.08984760620769805 1106 72-73 -0.0383325215360415 1106 74-75 -0.12073847624467504 1106 76-77 -0.14053052965072155 1106 78-79 -0.2615354675585273 1106 80-81 -0.3448559060583243 1106 82-83 -0.2558805981623564 1106 84-85 -0.3277973601564881 1106 86-87 -0.04546972992630671 1106 88-89 -0.16917231345840378 1106 90-91 -0.10669151439121904 1106 92-93 -0.11051224336021193 1106 94-95 -0.01950034731165573 1106 96-97 0.17791249551280686 1106 98-99 -0.04934821878270412 1106 100-101 0.18305916998053462 1107 1 -0.05047413861157679 1107 2 -0.028451074533819565 1107 3 0.018875418283478496 1107 4 -0.018310996724892448 1107 5 -0.042853801236745426 1107 6 0.08207726713612118 1107 7 0.09091929593591175 1107 8 0.009630226928223351 1107 9 0.1623624145205511 1107 10-11 0.08612194925802186 1107 12-13 0.21034951265554724 1107 14-15 0.13911657639365416 1107 16-17 0.1041251036983013 1107 18-19 0.08308145831305325 1107 20-21 0.09472603857141593 1107 22-23 0.11972627486905907 1107 24-25 0.154018345226568 1107 26-27 0.07479633225532467 1107 28-29 0.02998897075714524 1107 30-31 -0.08416397092431538 1107 32-33 -0.07070894629833901 1107 34-35 0.12049164927262268 1107 36-37 0.11729799057363977 1107 38-39 0.25763973479341473 1107 40-41 0.11373977512702993 1107 42-43 0.047407171946531435 1107 44-45 0.04216565080234602 1107 46-47 -0.003018286867749964 1107 48-49 -0.1661834374792832 1107 50-51 -0.14812275405768105 1107 52-53 -0.1909514479677341 1107 54-55 -0.06833796131559922 1107 56-57 -0.1499490185620047 1107 58-59 -0.145599796709746 1107 60-61 -0.4212422109075007 1107 62-63 -0.10601470102172783 1107 64-65 0.03374799301259657 1107 66-67 0.02167828877259126 1107 68-69 -0.16279029581575344 1107 70-71 -0.04835749321335214 1107 72-73 -0.1879664049734444 1107 74-75 -0.16086262135320695 1107 76-77 -0.11004249813124645 1107 78-79 -0.30113924394865776 1107 80-81 -0.2202461825971298 1107 82-83 -5.375265001461571E-4 1107 84-85 -0.008225920965291067 1107 86-87 0.05345608036212823 1107 88-89 -1.8257215599248866E-4 1107 90-91 0.057791462117847914 1107 92-93 0.14237766148665543 1107 94-95 0.018728168894110553 1107 96-97 -0.04530710008636163 1107 98-99 -0.040269643106224606 1107 100-101 0.2552324527637637 1108 1 0.094690602509516 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2316 50-51 -0.8900083192769301 2316 52-53 -0.7292793454757884 2316 54-55 -0.6387338972368113 2316 56-57 -0.4961278199092405 2316 58-59 -0.788009093036834 2316 60-61 -0.7358386008800082 2316 62-63 -0.7575123508266799 2316 64-65 -0.7358556180807483 2316 66-67 -0.5666405490266442 2316 68-69 -0.47705360825846554 2316 70-71 -0.4744156139099829 2316 72-73 -0.5672624389526817 2316 74-75 -0.6331086031058888 2316 76-77 -0.5022819249181403 2316 78-79 -0.555443271788608 2316 80-81 -0.6537892215432173 2316 82-83 -0.973676822187727 2316 84-85 -0.7800738084543646 2316 86-87 -0.8377441445318219 2316 88-89 -0.7651490778795988 2316 90-91 -0.6971560715096601 2316 92-93 -0.7237258376926441 2316 94-95 -0.9301324394919845 2316 96-97 -0.8580355912718005 2316 98-99 -1.122657036254953 2316 100-101 -1.4334366493657988 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 5.0 5 29.0 6 41.0 7 97.0 8 235.0 9 534.0 10 959.0 11 1574.0 12 2563.0 13 3643.0 14 4753.0 15 6862.0 16 9747.0 17 14573.0 18 12679.0 19 6749.0 20 5026.0 21 4970.0 22 4947.0 23 5378.0 24 5968.0 25 7110.0 26 8411.0 27 10506.0 28 13041.0 29 16189.0 30 20383.0 31 25834.0 32 33174.0 33 44160.0 34 60607.0 35 88512.0 36 134113.0 37 206864.0 38 201055.0 39 24022.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.44615459598431 20.093355455415 14.960836198440758 19.499653750159926 2 14.312464034636358 23.48285790547871 40.06513600108797 22.139542058796962 3 21.213957250566565 28.28422760861159 30.45671317933307 20.045101961488772 4 11.725620381581232 17.684795107381248 39.65692398720823 30.93266052382929 5 11.714451304735292 41.19134933451345 33.460580845477 13.633618515274268 6 27.489158377506666 38.46484858264751 18.497284646760505 15.548708393085315 7 25.61738637989776 36.706846384383596 20.58807111982568 17.08769611589296 8 25.01159518249346 34.185236719329595 20.353833210023513 20.449334888153427 9 26.832013461575542 18.427764296580918 21.51030677249998 33.22991546934356 10-11 23.342954543839937 28.075717302853988 27.18164500371959 21.399683149586483 12-13 27.83681671821755 25.608955897987613 27.31680829415212 19.237419089642714 14-15 24.007457453038242 26.498721738962754 28.7956842578525 20.6981365501465 16-17 19.419368065091348 30.293670197488538 29.866246905836967 20.420714831583144 18-19 19.06364714920894 29.601257658656095 32.759156134587336 18.57593905754763 20-21 21.011993021577695 27.86279220113607 31.53701882529891 19.588195951987316 22-23 20.15806144849403 27.744077262758125 30.753730032994593 21.344131255753247 24-25 20.357133433064252 27.92291422667606 31.902695373473332 19.817256966786356 26-27 21.50793763561777 26.807306562631304 32.60900674511269 19.07574905663824 28-29 19.797269867045568 27.272911803511622 33.621232112047096 19.308586217395717 30-31 19.745534187405298 29.089513142444222 32.44456871327966 18.72038395687082 32-33 19.384455655658094 28.79527829960328 31.900046989667345 19.920219055071282 34-35 20.175922007301157 27.46845450679625 32.01001093042586 20.345612555476734 36-37 21.06884855915122 27.65781135506971 32.341863878001305 18.931476207777763 38-39 21.74268919195958 26.598766697587184 32.89944809920006 18.759096011253174 40-41 20.196422898886965 27.737148123001287 33.23509143702116 18.831337541090587 42-43 19.31273709986424 29.116865650811373 31.973660504713454 19.596736744610936 44-45 20.175008601240403 29.10345135554534 30.50278944061998 20.218750602594277 46-47 20.1290338295158 28.814256847754493 29.438569890279638 21.61813943245007 48-49 21.001082893629803 28.39119760728208 29.936985130764228 20.670734368323888 50-51 21.932351117349334 29.333477448511807 29.608412672798668 19.12575876134019 52-53 20.613718155080267 29.759713068418243 29.985629063392476 19.640939713109017 54-55 19.31869041928383 29.19438600337148 30.626048514040573 20.860875063304114 56-57 20.896599389235814 30.418451614343724 28.269613111639536 20.41533588478093 58-59 20.810333261275744 29.321247956253938 28.972377585827186 20.896041196643132 60-61 20.508198834290887 28.6042241985623 30.024824346940036 20.862752620206777 62-63 22.45598651406696 28.21988322610961 29.584664115219073 19.73946614460436 64-65 21.67827199811635 28.766150795221467 30.069682733479276 19.485894473182906 66-67 20.131926282041523 29.06432712927639 30.35639074699363 20.44735584168846 68-69 20.212813463199378 30.152041513290566 29.633632829031697 20.00151219447836 70-71 20.280151787789386 30.333860064161698 28.47660107396255 20.909387074086368 72-73 20.495360404659184 29.006681057886603 28.323504069223997 22.174454468230216 74-75 20.655155720509924 29.91491115096268 28.648879605976923 20.781053522550476 76-77 21.350765180555005 28.497152710329505 29.469371972439497 20.682710136675993 78-79 20.460803208694 28.252410630828674 30.27311856112158 21.013667599355745 80-81 20.017953503571924 30.39008528167921 29.135065354203647 20.45689586054522 82-83 20.036384118864937 30.028620143709656 28.849409328948973 21.085586408476434 84-85 20.293000515059788 28.672236754971088 28.77854712543355 22.25621560453557 86-87 20.76024816227775 28.975473017477515 28.959995859225856 21.304282961018874 88-89 21.646150551646514 27.666257663730576 29.700260726685563 20.987331057937347 90-91 20.209135798709966 28.154824434531513 30.293581799450898 21.342457967307624 92-93 19.459221365236935 29.429063083736295 29.87476422191583 21.236951329110937 94-95 19.451946214591562 29.8200184102066 28.807863005329214 21.92017236987262 96-97 19.154886265722002 29.10609008416529 28.372574272598932 23.36644937751377 98-99 20.063756810697992 28.411108624575192 28.400705770234946 23.124428794491866 100-101 20.455232894509802 29.207275606215934 28.59111255468377 21.746378944590493 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1175.0 1 825.5 2 578.0 3 717.5 4 622.5 5 611.0 6 1053.5 7 1995.5 8 2906.5 9 3251.5 10 2893.5 11 2330.0 12 2244.0 13 2356.5 14 2419.0 15 2451.5 16 2480.0 17 2546.5 18 2777.5 19 2987.5 20 3185.5 21 3628.0 22 4083.0 23 4638.0 24 5369.0 25 6383.5 26 7784.0 27 9470.0 28 11173.0 29 13157.5 30 15525.5 31 17621.5 32 20105.5 33 23135.5 34 26250.0 35 28378.5 36 30034.0 37 31545.0 38 31799.0 39 31322.5 40 30850.5 41 30383.0 42 30100.0 43 30758.5 44 32377.0 45 32614.5 46 32884.0 47 40320.5 48 77282.0 49 74209.0 50 37851.5 51 29614.5 52 21986.5 53 18752.0 54 16551.5 55 14465.0 56 12814.0 57 11285.5 58 9742.0 59 8566.0 60 7543.0 61 6309.5 62 4756.5 63 3109.5 64 1900.0 65 1334.0 66 939.5 67 640.0 68 460.0 69 328.0 70 249.5 71 203.5 72 184.0 73 206.5 74 167.5 75 107.5 76 82.0 77 57.5 78 43.0 79 26.5 80 15.0 81 10.5 82 7.0 83 3.5 84 1.5 85 0.5 86 1.0 87 2.5 88 2.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04922243771839285 2 0.0 3 0.0 4 2.029791246119293E-4 5 0.010554914479820323 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005125222896451214 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010148956230596464 24-25 5.074478115298232E-4 26-27 0.0017253225592013988 28-29 0.002334259933037187 30-31 4.059582492238586E-4 32-33 0.0 34-35 0.0 36-37 1.0148956230596465E-4 38-39 1.0148956230596465E-4 40-41 0.0 42-43 0.011214596634809093 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.0148956230596465E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.0446868691789394E-4 84-85 5.0744781152982326E-5 86-87 0.0 88-89 0.0 90-91 0.007916185859865242 92-93 0.0021820255895782397 94-95 0.0 96-97 0.0 98-99 0.0016745777780484165 100-101 0.011468320540574005 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 985323.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.91275994348669 #Duplication Level Percentage of deduplicated Percentage of total 1 77.62428242684916 42.62563586690981 2 13.553172983117845 14.884842689889993 3 4.293890004278626 7.073680530860677 4 1.8001702838302207 3.954092746134689 5 0.9099269457200178 2.4983299968216692 6 0.4962400457542683 1.634994630410939 7 0.30109856479527697 1.157390724555199 8 0.20055016345651663 0.8810210386011772 9 0.13665548616669698 0.6753716916149068 >10 0.6006541767723514 5.8942544638701335 >50 0.04140317976230867 1.5930914958060056 >100 0.030445757994829497 3.4406687006413943 >500 0.005198056162147752 1.9817664365646241 >1k 0.0053837010250816 6.783880107388347 >5k 5.569345888015449E-4 2.3066578786084233 >10k+ 3.712897258676966E-4 2.6143210013220055 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 12900 1.309215353746944 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 12745 1.2934844715895193 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8989 0.9122896755683162 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7217 0.7324501711621468 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 6421 0.651664479566599 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4906 0.49790779267306257 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 4407 0.4472645010823862 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 4402 0.44675705327085635 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4269 0.433258941484163 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 4079 0.41397592464602984 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 3512 0.3564313428185478 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 3411 0.3461808970256454 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 3381 0.34313621015646645 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 2849 0.28914376300969324 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 2616 0.2654966949924035 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2429 0.2465181468411881 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 2351 0.23860196098132286 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2179 0.22114575626469693 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1872 0.1899884606367658 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1826 0.18531994077069144 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1734 0.1759829010385427 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1720 0.17456204716625917 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1548 0.15710584244963327 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1474 0.14959561483899186 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1457 0.14787029227979048 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1347 0.13670644042613436 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1171 0.11884427746028459 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1161 0.11782938183722495 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1126 0.11427724715651619 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1117 0.11336384109576252 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1073 0.10889830035430006 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1069 0.1084923421050762 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1035 0.1050416969866734 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1025 0.10402680136361375 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 997 0.10118509361904675 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.0148956230596464E-4 0.0 0.0 0.0 6 0.0 1.0148956230596464E-4 0.0 1.0148956230596464E-4 0.0 7 0.0 1.0148956230596464E-4 0.0 1.0148956230596464E-4 0.0 8 0.0 1.0148956230596464E-4 0.0 1.0148956230596464E-4 0.0 9 0.0 1.0148956230596464E-4 0.0 1.0148956230596464E-4 0.0 10-11 0.0 1.0148956230596464E-4 0.0 1.5223434345894694E-4 0.0 12-13 0.0 1.0148956230596464E-4 0.0 2.0297912461192928E-4 0.0 14-15 0.0 1.0148956230596464E-4 0.0 3.552134680708763E-4 0.0 16-17 0.0 1.5223434345894694E-4 0.0 4.0595824922385856E-4 0.0 18-19 0.0 2.0297912461192928E-4 0.0 4.0595824922385856E-4 0.0 20-21 0.0 4.0595824922385856E-4 0.0 4.567030303768409E-4 0.0 22-23 0.0 5.581925926828055E-4 0.0 0.0011671299665185933 0.0 24-25 0.0 6.089373738357879E-4 0.0 0.0018775569026603458 0.0 26-27 0.0 6.089373738357879E-4 0.0 0.0023850047141901693 0.0 28-29 0.0 6.089373738357879E-4 0.0 0.0047700094283803385 0.0 30-31 0.0 6.089373738357879E-4 0.0 0.010098211449443482 0.0 32-33 0.0 6.089373738357879E-4 0.0 0.020044188555428017 0.0 34-35 0.0 6.089373738357879E-4 0.0 0.029431973068729744 0.0 36-37 0.0 6.089373738357879E-4 0.0 0.03841379933280761 0.0 38-39 0.0 6.089373738357879E-4 0.0 0.046329985192672854 0.0 40-41 0.0 6.089373738357879E-4 0.0 0.05891469091861247 0.0 42-43 0.0 6.089373738357879E-4 0.0 0.07718281213368611 0.0 44-45 0.0 6.089373738357879E-4 0.0 0.09276145994765168 0.0 46-47 0.0 6.089373738357879E-4 0.0 0.10930425860352391 0.0 48-49 0.0 6.089373738357879E-4 0.0 0.12899323369088106 0.0 50-51 0.0 6.089373738357879E-4 0.0 0.15619243638887959 0.0 52-53 0.0 6.089373738357879E-4 0.0 0.23063503034030466 0.0 54-55 0.0 6.089373738357879E-4 0.0 0.3278620310294188 0.0 56-57 0.0 6.089373738357879E-4 0.0 0.3941347152152137 0.0 58-59 0.0 6.089373738357879E-4 0.0 0.5170893199488898 0.0 60-61 0.0 7.104269361417526E-4 0.0 0.6902812580240185 0.0 62-63 0.0 7.104269361417526E-4 0.0 0.786341128746614 0.0 64-65 0.0 7.104269361417526E-4 0.0 0.861037446603804 0.0 66-67 0.0 7.104269361417526E-4 0.0 1.0802549011846878 0.0 68-69 0.0 7.611717172947348E-4 0.0 1.2722224082864197 0.0 70-71 0.0 8.119164984477171E-4 0.0 1.3677748311974853 0.0 72-73 0.0 8.119164984477171E-4 0.0 1.6021649753431109 0.0 74-75 0.0 8.119164984477171E-4 0.0 1.931650839369425 0.0 76-77 0.0 8.119164984477171E-4 0.0 2.231095792953174 0.0 78-79 0.0 8.626612796006994E-4 0.0 2.532063089971512 0.0 80-81 0.0 9.134060607536818E-4 0.0 2.9493881701736386 0.0 82-83 0.0 9.134060607536818E-4 0.0 3.3860470119950516 0.0 84-85 0.0 9.134060607536818E-4 0.0 3.89273365180758 0.0 86-87 0.0 0.0010148956230596464 0.0 4.619500407480593 0.0 88-89 0.0 0.0010148956230596464 0.0 5.327542338908155 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTAATA 50 0.0016149323 38.003696 2 GGAACGC 170 3.6379788E-9 27.942476 8 GGGAACG 205 9.567884E-10 25.48899 7 GAACGCA 175 1.485023E-7 24.429708 9 GAGTACT 6695 0.0 23.591463 12-13 TAGGACG 270 4.0017767E-11 22.872595 4 AGTACTT 6790 0.0 22.281963 12-13 GTACTTT 7200 0.0 21.934557 14-15 GTCCTAC 220 5.5952114E-8 21.606167 1 CGGTATA 110 0.0028754552 21.593008 2 CATGGGT 905 0.0 21.521431 4 GTACATG 10025 0.0 20.435879 1 GTATAGA 375 0.0 20.280989 1 TACATGG 9955 0.0 20.042128 2 CTGGTCG 120 0.004761744 19.792587 9 ACTTTTT 8155 0.0 19.744469 16-17 ACATGGG 10025 0.0 19.523096 3 TACCTGG 880 0.0 19.433708 2 TACTTTT 7825 0.0 19.302452 14-15 TATACTA 425 0.0 19.001846 5 >>END_MODULE