##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937381_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1073358 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.71999742863052 33.0 31.0 34.0 30.0 34.0 2 31.77881284715817 33.0 31.0 34.0 30.0 34.0 3 31.830575632733908 33.0 31.0 34.0 30.0 34.0 4 35.449787489355835 37.0 35.0 37.0 33.0 37.0 5 35.42274991195855 37.0 35.0 37.0 33.0 37.0 6 35.52680093687288 37.0 35.0 37.0 33.0 37.0 7 35.48533201410899 37.0 35.0 37.0 33.0 37.0 8 35.4923129095791 37.0 35.0 37.0 33.0 37.0 9 37.088952614132474 39.0 37.0 39.0 33.0 39.0 10-11 36.99305637075421 39.0 37.0 39.0 33.0 39.0 12-13 36.893377139780014 39.0 37.0 39.0 33.0 39.0 14-15 37.94607856838073 40.0 38.0 41.0 33.0 41.0 16-17 37.91922126634357 40.0 38.0 41.0 33.0 41.0 18-19 37.97911693954859 40.0 38.0 41.0 33.0 41.0 20-21 37.9564693233758 40.0 38.0 41.0 33.0 41.0 22-23 37.84265734265735 40.0 37.5 41.0 33.0 41.0 24-25 37.69971202525159 40.0 37.0 41.0 32.5 41.0 26-27 37.468792332101685 39.5 37.0 41.0 32.0 41.0 28-29 37.302346001986294 39.0 36.5 41.0 32.0 41.0 30-31 37.29373983330818 39.0 37.0 41.0 32.0 41.0 32-33 37.142184154774085 39.0 36.0 41.0 32.0 41.0 34-35 36.952760868228495 39.0 36.0 41.0 31.0 41.0 36-37 36.88547017863564 39.0 36.0 41.0 31.0 41.0 38-39 36.80862489495583 39.0 35.5 41.0 31.0 41.0 40-41 36.609946075773415 39.0 35.0 41.0 30.5 41.0 42-43 36.39565131111894 39.0 35.0 41.0 30.0 41.0 44-45 36.14951302361374 39.0 35.0 41.0 30.0 41.0 46-47 35.7113083426033 39.0 35.0 41.0 28.0 41.0 48-49 35.3438880597154 39.0 35.0 40.5 26.5 41.0 50-51 34.37938972831059 38.0 34.0 39.5 24.5 40.5 52-53 34.46590373388935 38.0 34.5 39.5 24.0 40.5 54-55 34.845886926822175 39.0 35.0 40.0 24.5 41.0 56-57 34.69743272980683 38.5 34.5 40.0 23.5 41.0 58-59 34.518996923673186 38.0 34.0 40.0 23.0 41.0 60-61 34.227195399857266 38.0 34.0 40.0 22.5 41.0 62-63 33.87892809295687 38.0 34.0 40.0 22.0 41.0 64-65 33.4824494716581 37.5 33.0 40.0 20.0 41.0 66-67 33.111266697597635 37.0 33.0 39.5 18.5 41.0 68-69 32.67794668693949 36.0 32.0 39.0 15.5 41.0 70-71 32.23203535074039 36.0 32.0 39.0 12.0 40.0 72-73 31.759971509971507 35.0 31.0 38.5 8.0 40.0 74-75 31.30871666303321 35.0 31.0 37.5 7.0 39.5 76-77 30.820235187141662 35.0 30.5 37.0 2.0 39.0 78-79 30.346612686540745 34.5 30.0 36.5 2.0 39.0 80-81 29.90486491925341 34.0 30.0 36.0 2.0 38.5 82-83 29.462849766806603 34.0 29.0 35.5 2.0 37.0 84-85 29.001889397572853 34.0 29.0 35.0 2.0 37.0 86-87 28.51664216412418 34.0 29.0 35.0 2.0 36.0 88-89 28.060816148945644 34.0 27.5 35.0 2.0 36.0 90-91 27.576380387531465 33.5 26.5 35.0 2.0 36.0 92-93 27.181888987644385 33.0 26.0 35.0 2.0 35.0 94-95 26.68438955129603 33.0 25.0 35.0 2.0 35.0 96-97 26.02286096530701 33.0 24.5 35.0 2.0 35.0 98-99 25.29502458639149 32.0 19.5 35.0 2.0 35.0 100-101 23.494354167016038 30.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19956194875151567 1101 2 0.11512768402701568 1101 3 0.23774700792572645 1101 4 0.2076634643354467 1101 5 0.28005213563404396 1101 6 0.25190917427146786 1101 7 0.2771550516948835 1101 8 0.1255468893765297 1101 9 0.1568165956478893 1101 10-11 0.21489516604894732 1101 12-13 0.2904731674213039 1101 14-15 0.35691706991028127 1101 16-17 0.40930550627746953 1101 18-19 0.36286194178192943 1101 20-21 0.34731350501710523 1101 22-23 0.35689328572394885 1101 24-25 0.4024780237188992 1101 26-27 0.4042277910050913 1101 28-29 0.3855097015637625 1101 30-31 0.4488981226417579 1101 32-33 0.5712031791830796 1101 34-35 0.2967928098868171 1101 36-37 0.4659715487697369 1101 38-39 0.4174178501367578 1101 40-41 0.47444167632860257 1101 42-43 0.6560363396329336 1101 44-45 0.6254379213943224 1101 46-47 0.6334999191964314 1101 48-49 0.7875627140314876 1101 50-51 0.6416052858492023 1101 52-53 0.5898524851785041 1101 54-55 0.8523383988826012 1101 56-57 0.8542491717651259 1101 58-59 0.808939223468947 1101 60-61 0.8141178160501354 1101 62-63 0.7447890610723391 1101 64-65 0.6253460986126669 1101 66-67 0.7681857709837914 1101 68-69 0.599565385589031 1101 70-71 0.8491743813044508 1101 72-73 0.7784186538109346 1101 74-75 0.8794662749185811 1101 76-77 0.9341674927170089 1101 78-79 0.9936298683140947 1101 80-81 1.1163349610486115 1101 82-83 1.0433573914466905 1101 84-85 0.8690273614306108 1101 86-87 1.0035982462918014 1101 88-89 1.167800569542191 1101 90-91 1.0787562673911708 1101 92-93 1.0561801635594428 1101 94-95 0.9051841544142007 1101 96-97 0.9447273114646926 1101 98-99 1.2236853196442894 1101 100-101 1.5370346386230622 1102 1 0.014388701370371848 1102 2 0.04075218040090789 1102 3 -0.012753740318778739 1102 4 -0.0723871860616967 1102 5 -0.055241982013015445 1102 6 0.04692471473423154 1102 7 -0.04133234326310031 1102 8 0.0741252248202997 1102 9 0.20425529715588908 1102 10-11 0.006169483995307701 1102 12-13 0.20717628702530533 1102 14-15 0.21120508648677827 1102 16-17 0.21339207241077673 1102 18-19 0.1939716459375589 1102 20-21 0.34160957961132254 1102 22-23 0.3348661186927515 1102 24-25 0.25515443674986926 1102 26-27 0.3317574329988773 1102 28-29 0.2847154112448891 1102 30-31 0.22901208603533263 1102 32-33 0.09103165852002348 1102 34-35 -0.008813268160835719 1102 36-37 0.19900942145304867 1102 38-39 0.2500700891145442 1102 40-41 0.29114479593260967 1102 42-43 0.19266578593200023 1102 44-45 0.14552540844393036 1102 46-47 0.24528764798657932 1102 48-49 0.40921000308409106 1102 50-51 0.36583689630160166 1102 52-53 0.43635875892088194 1102 54-55 0.5162384794627783 1102 56-57 0.5918168015567673 1102 58-59 0.6620588274749295 1102 60-61 0.6475240552581383 1102 62-63 0.5477877948124075 1102 64-65 0.4903397673130243 1102 66-67 0.6695441225435914 1102 68-69 0.7057470363315232 1102 70-71 0.6000262288928013 1102 72-73 0.46759213142115996 1102 74-75 0.5362868113523369 1102 76-77 0.6715336720654648 1102 78-79 0.8611272782369674 1102 80-81 0.8187869007285933 1102 82-83 0.732732319500105 1102 84-85 0.6950892930525718 1102 86-87 0.7158003874222238 1102 88-89 0.9259506789010032 1102 90-91 0.8423743173508811 1102 92-93 0.7102494625119 1102 94-95 0.7200396856677997 1102 96-97 0.9941229601351189 1102 98-99 1.1365666365546154 1102 100-101 1.4009750092918765 1103 1 0.12097357249291463 1103 2 0.0027518835622402094 1103 3 0.15867271083359213 1103 4 -0.0653379744510687 1103 5 -0.0019445991406001895 1103 6 0.017976583757700837 1103 7 0.16930366892142246 1103 8 0.09395051406130506 1103 9 0.085072451603871 1103 10-11 0.1954119093085751 1103 12-13 0.22365295338570945 1103 14-15 0.1680426238566426 1103 16-17 0.21940093516528947 1103 18-19 0.13146908393715506 1103 20-21 0.07442050044728177 1103 22-23 0.01669075771572892 1103 24-25 0.18574648854114884 1103 26-27 0.11756465748664624 1103 28-29 -0.13192380962428274 1103 30-31 0.057474320382389976 1103 32-33 0.19930532963691405 1103 34-35 0.14969434009693572 1103 36-37 0.141491982181293 1103 38-39 0.20807271825703566 1103 40-41 0.46139873434565004 1103 42-43 0.38365602559388634 1103 44-45 0.3589594784554535 1103 46-47 0.38441805204055157 1103 48-49 0.4081374967055993 1103 50-51 0.32901688654833094 1103 52-53 0.36814381945476526 1103 54-55 0.559863869063534 1103 56-57 0.4200514595915621 1103 58-59 0.5696879346266215 1103 60-61 0.5183292654835228 1103 62-63 0.5069480947608085 1103 64-65 0.4251334997765781 1103 66-67 0.480112992648408 1103 68-69 0.45080007857449544 1103 70-71 0.5178729470586205 1103 72-73 0.5396705675054285 1103 74-75 0.45160722042429313 1103 76-77 0.5171704970225264 1103 78-79 0.6829756643533997 1103 80-81 0.6099566571088566 1103 82-83 0.7639830704312587 1103 84-85 0.4509006167410057 1103 86-87 0.41065589059910224 1103 88-89 0.6390868501062386 1103 90-91 0.7548093838922192 1103 92-93 0.746730934831696 1103 94-95 0.7813160212651198 1103 96-97 0.6896456435566805 1103 98-99 0.8522262898187805 1103 100-101 1.197370893129012 1104 1 0.07131669148060027 1104 2 0.1854518816474311 1104 3 0.029847328129616102 1104 4 0.14329810732009918 1104 5 0.12408221701861777 1104 6 0.09584964372509575 1104 7 0.27251922188134614 1104 8 0.22777575683695517 1104 9 0.36537319440235194 1104 10-11 0.2355422488630694 1104 12-13 0.2915513330156827 1104 14-15 0.28093532534239074 1104 16-17 0.1607982152531946 1104 18-19 0.28272276810271535 1104 20-21 0.18266463560920698 1104 22-23 0.30911813123156406 1104 24-25 0.26369172206678826 1104 26-27 0.34672233314456946 1104 28-29 0.39466846426995517 1104 30-31 0.21631211988424326 1104 32-33 0.42062444134243293 1104 34-35 0.3750275536319734 1104 36-37 0.2352307050628113 1104 38-39 0.4594624274859669 1104 40-41 0.3421367163435036 1104 42-43 0.4318907929225375 1104 44-45 0.49194331010547643 1104 46-47 0.6616190141582692 1104 48-49 0.7505321711807582 1104 50-51 0.5962295437283487 1104 52-53 0.5631862324672241 1104 54-55 0.8220950777938825 1104 56-57 0.8339432493337782 1104 58-59 0.465656378579439 1104 60-61 0.7807070307250328 1104 62-63 0.8610072682410035 1104 64-65 0.8712923935474066 1104 66-67 0.9000927693227965 1104 68-69 0.8880300721377878 1104 70-71 0.7702082771937313 1104 72-73 0.9103771613700893 1104 74-75 0.9576234020306451 1104 76-77 0.9659162236850598 1104 78-79 0.8878620351870339 1104 80-81 0.9769658290424736 1104 82-83 0.9691971681365779 1104 84-85 0.8283326078916424 1104 86-87 0.8206981784125276 1104 88-89 0.9054669337391879 1104 90-91 0.9592693415602156 1104 92-93 1.1533935853098818 1104 94-95 1.4040703557291678 1104 96-97 1.5321007081520115 1104 98-99 1.5604963485444898 1104 100-101 1.649141281054522 1105 1 0.17169568666448853 1105 2 0.1684176215773796 1105 3 0.2178364645131552 1105 4 0.1691470838962843 1105 5 0.16791978269287 1105 6 0.1810961958266688 1105 7 0.1345194818419415 1105 8 0.2450203797572712 1105 9 0.32574459600042616 1105 10-11 0.35678906776631436 1105 12-13 0.39278717200429725 1105 14-15 0.4273692474636661 1105 16-17 0.3076237885347197 1105 18-19 0.49633427067495717 1105 20-21 0.4625301744117465 1105 22-23 0.5149992266234236 1105 24-25 0.5453650131679311 1105 26-27 0.37757298578248566 1105 28-29 0.3118154309402712 1105 30-31 0.3412240411298697 1105 32-33 0.22912498178683194 1105 34-35 0.3303280133517674 1105 36-37 0.1917369030706979 1105 38-39 0.23230731994437548 1105 40-41 0.22932921032336395 1105 42-43 0.28300520294880727 1105 44-45 0.2864786647464541 1105 46-47 0.19081925906851183 1105 48-49 0.33481071211770796 1105 50-51 0.14678161794025613 1105 52-53 0.08835537788096559 1105 54-55 0.10561118166866379 1105 56-57 0.3632888669454246 1105 58-59 0.35677308036358824 1105 60-61 0.38024628477494815 1105 62-63 0.34642553115694596 1105 64-65 0.28621686815336034 1105 66-67 0.3958333491717454 1105 68-69 0.3210782469407647 1105 70-71 0.27002442348313593 1105 72-73 0.26193709596947556 1105 74-75 0.15733214354771619 1105 76-77 0.16149723561588303 1105 78-79 0.3386696232999924 1105 80-81 0.21462625209413844 1105 82-83 0.23428086860724662 1105 84-85 0.07399964625635391 1105 86-87 0.15223601636130013 1105 88-89 0.321007959625792 1105 90-91 0.17143566845382452 1105 92-93 0.33573199927660724 1105 94-95 0.4425547854577161 1105 96-97 0.4990976843998247 1105 98-99 0.6366724992121782 1105 100-101 0.8827535019389359 1106 1 0.21528667117776124 1106 2 0.196556449011009 1106 3 0.11805424659280206 1106 4 0.23304711155115143 1106 5 0.4148777472946392 1106 6 0.24390731308330516 1106 7 0.1606792578375149 1106 8 0.21535189247850894 1106 9 0.19141972044732825 1106 10-11 0.20046560952516757 1106 12-13 0.1938536128228847 1106 14-15 0.3748466050410997 1106 16-17 0.3796949652493211 1106 18-19 0.226885121062125 1106 20-21 0.3972344401750192 1106 22-23 0.26207386019643053 1106 24-25 0.1932623790418262 1106 26-27 0.3183613223422199 1106 28-29 0.5935277857522152 1106 30-31 0.3749154473798697 1106 32-33 0.3436809483244403 1106 34-35 0.2579413983075227 1106 36-37 0.2553863084910475 1106 38-39 0.1041918263049979 1106 40-41 0.24472087238088847 1106 42-43 0.18934162440898916 1106 44-45 0.372969262091587 1106 46-47 0.24567528091585444 1106 48-49 0.22037141455133735 1106 50-51 0.36772672302875264 1106 52-53 0.45542620890972785 1106 54-55 0.7413360848766288 1106 56-57 0.660963265452061 1106 58-59 0.4401654683613785 1106 60-61 0.4094653804607944 1106 62-63 0.6426955809357011 1106 64-65 0.667715761958668 1106 66-67 0.6874496033199762 1106 68-69 0.5749585705027087 1106 70-71 0.6679995686711919 1106 72-73 0.8393404767770001 1106 74-75 0.8226434336472757 1106 76-77 0.6866718069654425 1106 78-79 0.8620466282778665 1106 80-81 0.8640748831782084 1106 82-83 0.7083328251891068 1106 84-85 0.5050050735240532 1106 86-87 0.6681159596688246 1106 88-89 0.9119103725129598 1106 90-91 0.9482306421816986 1106 92-93 0.8066771043651038 1106 94-95 0.8989810852364783 1106 96-97 1.0856833453511534 1106 98-99 1.1989866930728006 1106 100-101 1.1632602773814114 1107 1 -0.02698360587558568 1107 2 -0.06912669099968483 1107 3 -0.17912475234874492 1107 4 -0.11357919924181914 1107 5 -0.025090389933517088 1107 6 -0.03313103136198947 1107 7 -0.0707814101895039 1107 8 -0.102336037859871 1107 9 -0.27723036739353546 1107 10-11 0.024052790215449704 1107 12-13 0.15649396724165854 1107 14-15 0.2921623029121889 1107 16-17 0.10892824002208101 1107 18-19 0.10384585456059625 1107 20-21 -0.1549156149301112 1107 22-23 -0.019857793066769602 1107 24-25 0.1400211331201504 1107 26-27 0.06183804724209807 1107 28-29 -0.13889585817039318 1107 30-31 0.010473400524141141 1107 32-33 0.04537529486481873 1107 34-35 0.2124469251890062 1107 36-37 0.0964925577408593 1107 38-39 0.0906160485587435 1107 40-41 0.09164112270006086 1107 42-43 -0.05317553456660562 1107 44-45 -0.1633421583115009 1107 46-47 -0.20665673025794007 1107 48-49 -0.15898311880091143 1107 50-51 -0.3015340224728149 1107 52-53 -0.4869043564362343 1107 54-55 -0.2026851442407036 1107 56-57 -0.3049622553187561 1107 58-59 -0.3061135709542313 1107 60-61 -0.3496119631442909 1107 62-63 -0.3105782643116086 1107 64-65 -0.28520344627327177 1107 66-67 -0.3360532828797318 1107 68-69 -0.14937321152100935 1107 70-71 -0.15005459258498632 1107 72-73 0.07027737321207539 1107 74-75 0.21292215106158707 1107 76-77 0.24757321241921204 1107 78-79 0.3307451377112116 1107 80-81 0.21227455472977041 1107 82-83 0.23341930206131778 1107 84-85 0.2746362783078311 1107 86-87 0.5159609942050096 1107 88-89 0.33811586556752715 1107 90-91 0.47310979279876975 1107 92-93 0.23760131570693943 1107 94-95 0.1374458343171483 1107 96-97 0.2661110648714633 1107 98-99 0.5668073394942361 1107 100-101 0.8826932946331425 1108 1 -0.03757389512848874 1108 2 -0.2223662277756695 1108 3 -0.27226072240921084 1108 4 -0.1904986820464174 1108 5 -0.2585474076953034 1108 6 -0.11045735084616126 1108 7 -4.898325064885967E-4 1108 8 -0.07023240732683433 1108 9 0.00970117236364132 1108 10-11 -0.08948826154284006 1108 12-13 -0.14559480292638938 1108 14-15 -0.06705568177616783 1108 16-17 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11 2558.0 12 3655.0 13 5130.0 14 6050.0 15 8036.0 16 10593.0 17 14542.0 18 11862.0 19 7304.0 20 6903.0 21 7040.0 22 7683.0 23 8468.0 24 9454.0 25 10719.0 26 12361.0 27 14594.0 28 17389.0 29 20958.0 30 25493.0 31 32073.0 32 40738.0 33 53217.0 34 71332.0 35 100356.0 36 141337.0 37 198579.0 38 184430.0 39 29459.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.87103242643713 19.0479972566055 14.652118702269748 20.428851614687627 2 13.851887832308929 21.89736021073477 41.37808956227698 22.87266239467932 3 20.535879428529793 27.62694511546919 30.79013397452784 21.047041481473176 4 11.226769897037421 16.78433907589637 40.52729321074727 31.461597816318942 5 11.92424301862261 39.86983413566168 34.38896952646749 13.81695331924822 6 27.60295800653808 37.454516262128216 19.52659573475023 15.415929996583468 7 25.534546690216843 36.018514023682116 21.163138496697126 17.283800789403912 8 24.402040279082097 33.17227177084948 21.36607961345225 21.059608336616176 9 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28.24756646648226 31.7628052827772 20.806699907298405 34-35 20.095771939568465 26.92825038854844 31.917072328350265 21.058905343532828 36-37 20.98702627322021 26.87658857912536 32.47557450208575 19.660810645568688 38-39 21.680536025720425 26.115479623452853 32.75629768151235 19.447686669314376 40-41 20.13502375936537 27.014179081491218 33.52646665428373 19.32433050485968 42-43 19.25174318999686 28.55858803126985 31.998766081426496 20.1909026973068 44-45 19.90226142967164 28.785642605428365 30.69140470022638 20.62069126467362 46-47 19.84021938798369 28.196498976985918 29.536918114069806 22.42636352096059 48-49 20.5340319463289 27.82004087688813 30.302852094088724 21.343075082694252 50-51 21.639603509341875 28.5000998881626 30.15548946133208 19.704807141163442 52-53 20.130233252890573 28.832615261096667 30.774817836821 20.26233364919176 54-55 19.203309693774905 28.231962369644396 31.432241693907475 21.132486242673224 56-57 20.579000813864063 29.730024863920434 28.958067334184527 20.732906988030972 58-59 20.23649372229136 28.510134345344667 29.748551019827264 21.504820912536708 60-61 20.33374305732496 27.512592712886146 30.796824499068087 21.356839730720807 62-63 22.190297615936565 27.385230081161193 30.07504797246343 20.349424330438808 64-65 21.298618755783572 27.956160813990984 30.85906493140444 19.886155498821005 66-67 19.952518228025447 28.21991891603044 30.998540637891054 20.829022218053062 68-69 19.752579436373903 29.591770069671615 30.435389260398242 20.22026123355624 70-71 19.923007423917447 29.34300103975912 29.164356609377247 21.569634926946186 72-73 19.965173007820905 28.388712544764726 28.99708810500931 22.649026342405058 74-75 20.340105450516752 28.742032643179083 29.67459490033162 21.243267005972545 76-77 21.107593622221692 27.491299232204202 30.23097347321769 21.17013367235641 78-79 20.113858900092037 27.49583226767576 31.17015735639759 21.22015147583462 80-81 19.805876168240655 29.32232125398825 30.028153975599885 20.84364860217121 82-83 19.554132784719204 29.082600621391375 29.664040742813324 21.699225851076093 84-85 20.055717715573664 27.66524572492528 29.562261280620827 22.716775278880235 86-87 20.65627137879895 27.979825413669552 30.012217123198816 21.351686084332684 88-89 21.57742692733102 26.548701889645454 30.401870510983652 21.472000672039872 90-91 19.924892208138278 27.14618858523969 31.41077484602996 21.51814436059207 92-93 19.530970341334374 28.4685666868439 30.825522628447843 21.17494034337388 94-95 19.303601188962197 28.9561951716262 29.908214321216743 21.831989318194864 96-97 19.01909689004705 28.08379634339496 29.275940307868638 23.62116645868936 98-99 19.93806107648281 27.594369120889173 29.488051836046665 22.979517966581355 100-101 20.818218215789283 27.46386137551225 30.299888287343247 21.418032121355225 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2021.0 1 1260.0 2 573.0 3 675.5 4 588.5 5 583.0 6 1163.0 7 2238.5 8 3361.5 9 3730.5 10 3018.5 11 2376.5 12 2338.5 13 2403.5 14 2403.5 15 2394.5 16 2447.5 17 2522.5 18 2753.0 19 2975.5 20 3161.5 21 3574.5 22 4107.0 23 4692.5 24 5529.0 25 6664.5 26 8146.5 27 10076.5 28 11897.0 29 14145.0 30 16745.5 31 19111.0 32 21762.5 33 24733.5 34 28272.0 35 30954.5 36 32525.0 37 34219.5 38 34584.5 39 33868.0 40 33400.5 41 33061.0 42 33249.0 43 33960.0 44 35371.5 45 35877.0 46 36870.5 47 45414.5 48 81635.0 49 78667.0 50 42059.5 51 32419.5 52 24746.0 53 21160.5 54 18833.0 55 16470.5 56 14484.0 57 12714.0 58 11061.5 59 9608.5 60 8314.0 61 6993.5 62 5468.0 63 3660.0 64 2302.0 65 1630.0 66 1066.5 67 748.0 68 551.0 69 380.0 70 306.0 71 253.0 72 220.0 73 234.5 74 188.0 75 124.5 76 92.5 77 50.0 78 39.5 79 27.5 80 11.0 81 8.0 82 8.0 83 5.0 84 3.0 85 2.5 86 2.0 87 3.0 88 5.0 89 5.5 90 3.5 91 2.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2935646820538907 2 0.2625405503103345 3 0.26608084162040996 4 0.26757149059307334 5 0.25378298759593726 6 0.19527501541890033 7 0.20216926691746834 8 0.19862897560739287 9 0.19490235317573448 10-11 0.2045915714980463 12-13 0.20524373042358654 14-15 0.19112914796368036 16-17 0.20156369077232386 18-19 0.18777518777518776 20-21 0.19303904195990526 22-23 0.1919676380108035 24-25 0.18684353216727317 26-27 0.1956942604424619 28-29 0.1947626048345473 30-31 0.2022624324782598 32-33 0.2030077569645915 34-35 0.19220055191278212 36-37 0.1899645784537871 38-39 0.18553921431619275 40-41 0.185492631535797 42-43 0.18395539978273792 44-45 0.19872214116818435 46-47 0.18763543943400057 48-49 0.1949489359561302 50-51 0.20123761130955375 52-53 0.20524373042358654 54-55 0.20510398208239936 56-57 0.1794368700843521 58-59 0.17035322790718474 60-61 0.17748039330773144 62-63 0.17794622111168873 64-65 0.17668848604100404 66-67 0.18628453880252444 68-69 0.17729406218614852 70-71 0.1822318369080959 72-73 0.1921539691323864 74-75 0.18213867134730444 76-77 0.19047698903814012 78-79 0.18931241952824687 80-81 0.17966978398633074 82-83 0.1912223135244718 84-85 0.17566366487229795 86-87 0.1780393866724802 88-89 0.18567896265737993 90-91 0.1830237441748233 92-93 0.18805468445756215 94-95 0.18665720104569025 96-97 0.17953003564514355 98-99 0.18446781036709095 100-101 0.17319477751132425 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1073358.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.00996675462645 #Duplication Level Percentage of deduplicated Percentage of total 1 78.16474727268906 43.780048961270914 2 13.12267077533242 14.700007077165536 3 4.271239448741793 7.176959385752305 4 1.7942143393756826 4.019755419964242 5 0.8845915741939232 2.477297233101215 6 0.4774881942958123 1.6046458732941038 7 0.2862942117459923 1.122473049735451 8 0.19177099533944636 0.8592869658771211 9 0.12377942831686649 0.6239593498440923 >10 0.6068064300006326 5.9707206947246325 >50 0.0370421773299142 1.4107414695406761 >100 0.028893657580373346 3.1974161488519774 >500 0.004649553359104771 1.7945819801264067 >1k 0.004649553359104771 5.660722625365819 >5k 8.302773855544235E-4 3.272007984495827 >10k+ 3.3211095422176935E-4 2.3293757808896527 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 13419 1.2501886602606027 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 11626 1.0831428097615148 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9041 0.8423098351155905 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 8722 0.8125900212231147 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6447 0.6006383704225431 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 5616 0.5232177894048398 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 5354 0.49880841247747726 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 4963 0.4623806782080163 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4456 0.41514573888674605 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 4224 0.39353132878312735 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 3702 0.3448989060499852 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3267 0.3043718871057 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2815 0.2622610536279601 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2409 0.22443583594662733 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2400 0.22359734589950417 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2324 0.2165167632793532 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 2310 0.21521244542827278 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2162 0.20142394243113665 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 2151 0.20039912126243062 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 2108 0.19639300214839783 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1922 0.17906420784118626 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1850 0.17235628746420112 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1835 0.17095880405232924 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1644 0.15316418194116035 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1628 0.151673532968497 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1473 0.1372328710458207 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1465 0.136487546559489 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1438 0.1339720764181196 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1359 0.12661199711559423 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1302 0.12130156015048102 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1225 0.11412781196953858 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1185 0.1104011895378802 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1098 0.10229578574902315 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1082 0.1008051367763598 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 4.6582780395730035E-5 0.0 9.316556079146007E-5 0.0 20-21 0.0 1.8633112158292014E-4 0.0 3.726622431658403E-4 0.0 22-23 0.0 2.794966823743802E-4 0.0 6.987417059359505E-4 0.0 24-25 0.0 2.794966823743802E-4 0.0 0.0011645695098932507 0.0 26-27 0.0 2.794966823743802E-4 0.0 0.0020962251178078518 0.0 28-29 0.0 2.794966823743802E-4 0.0 0.004891191941551654 0.0 30-31 0.0 2.794966823743802E-4 0.0 0.010108463345873418 0.0 32-33 0.0 2.794966823743802E-4 0.0 0.018586529377896285 0.0 34-35 0.0 2.794966823743802E-4 0.0 0.02631927092358747 0.0 36-37 0.0 2.794966823743802E-4 0.0 0.03540291310075483 0.0 38-39 0.0 2.794966823743802E-4 0.0 0.044346806936735 0.0 40-41 0.0 2.794966823743802E-4 0.0 0.05864772051822412 0.0 42-43 0.0 2.794966823743802E-4 0.0 0.07634917706860153 0.0 44-45 0.0 2.794966823743802E-4 0.0 0.09423696474056187 0.0 46-47 0.0 2.794966823743802E-4 0.0 0.11519921591864038 0.0 48-49 0.0 2.794966823743802E-4 0.0 0.134763983684847 0.0 50-51 0.0 2.794966823743802E-4 0.0 0.16303973138505512 0.0 52-53 0.0 2.794966823743802E-4 0.0 0.23668710719070432 0.0 54-55 0.0 2.794966823743802E-4 0.0 0.3355357671904434 0.0 56-57 0.0 3.726622431658403E-4 0.0 0.40438511661533244 0.0 58-59 0.0 4.6582780395730035E-4 0.0 0.5336057494330876 0.0 60-61 0.0 4.6582780395730035E-4 0.0 0.7113656394231933 0.0 62-63 0.0 4.6582780395730035E-4 0.0 0.806674008112857 0.0 64-65 0.0 4.6582780395730035E-4 0.0 0.8812996223068166 0.0 66-67 0.0 4.6582780395730035E-4 0.0 1.0994467829000203 0.0 68-69 0.0 5.589933647487604E-4 0.0 1.295327374464065 0.0 70-71 0.0 6.521589255402205E-4 0.0 1.3915208159812478 0.0 72-73 0.0 6.521589255402205E-4 0.0 1.6186118704104315 0.0 74-75 0.0 6.987417059359505E-4 0.0 1.9338841281287324 0.0 76-77 0.0 7.453244863316806E-4 0.0 2.2242811811157135 0.0 78-79 0.0 8.384900471231407E-4 0.0 2.514119240737946 0.0 80-81 0.0 9.316556079146008E-4 0.0 2.916454715015866 0.0 82-83 0.0 0.0010248211687060608 0.0 3.336072400820602 0.0 84-85 0.0 0.0010248211687060608 0.0 3.827380985654367 0.0 86-87 0.0 0.0010248211687060608 0.0 4.506744254945694 0.0 88-89 0.0 0.0011179867294975208 0.0 5.169850133878911 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACAGCG 60 0.0039482177 31.670948 7 TGGGACG 105 6.899049E-5 27.146526 6 CATTCCG 95 0.0012265351 24.999876 9 CATGGGG 2150 0.0 23.437098 4 GAGTACT 5920 0.0 22.946451 12-13 GTACATG 10690 0.0 22.020458 1 CATGGGT 1115 0.0 21.743614 4 AGTACTT 6005 0.0 21.59339 12-13 TACATGG 10755 0.0 21.480381 2 GTATAGC 540 0.0 21.135685 1 GTACTTT 6485 0.0 20.910639 14-15 ACATGGG 10850 0.0 20.765598 3 CGGGAGT 275 1.2460077E-9 20.743643 4 GTGTAAG 380 0.0 20.023281 1 CGGGTCG 190 8.240748E-6 20.002705 6 ATAGCCC 490 0.0 19.40125 3 TAGCCCT 820 0.0 19.13095 4 ATGGGAG 1800 0.0 18.487673 5 GGAACGC 180 1.2319429E-4 18.47472 8 ACTTTTT 7430 0.0 18.442856 16-17 >>END_MODULE