##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937381_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1073358 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04767654407942 31.0 31.0 34.0 31.0 34.0 2 32.23619332971851 33.0 31.0 34.0 30.0 34.0 3 32.32110442182385 34.0 31.0 34.0 30.0 34.0 4 35.93238788922242 37.0 35.0 37.0 35.0 37.0 5 35.83942170273105 37.0 35.0 37.0 35.0 37.0 6 35.95384112290587 37.0 35.0 37.0 35.0 37.0 7 35.90997132364039 37.0 35.0 37.0 35.0 37.0 8 35.91798822014649 37.0 35.0 37.0 35.0 37.0 9 37.563535185837345 39.0 37.0 39.0 35.0 39.0 10-11 37.48900786131002 39.0 37.0 39.0 35.0 39.0 12-13 37.41758667657948 39.0 37.0 39.0 34.0 39.0 14-15 38.47567540373296 40.0 38.0 41.0 34.0 41.0 16-17 38.48300660171164 40.0 38.0 41.0 34.0 41.0 18-19 38.599150050588904 40.0 38.0 41.0 34.0 41.0 20-21 38.53266617475251 40.0 38.0 41.0 34.0 41.0 22-23 38.43120608408378 40.0 38.0 41.0 34.0 41.0 24-25 38.28553287905806 40.0 38.0 41.0 34.0 41.0 26-27 38.14892840971977 40.0 37.5 41.0 33.5 41.0 28-29 38.038306883630625 40.0 37.0 41.0 33.0 41.0 30-31 38.089156646710606 40.0 37.0 41.0 34.0 41.0 32-33 37.97564745406472 40.0 37.0 41.0 33.5 41.0 34-35 37.85970058452072 40.0 37.0 41.0 33.0 41.0 36-37 37.65660804689581 40.0 36.5 41.0 33.0 41.0 38-39 37.466376083282555 40.0 36.0 41.0 33.0 41.0 40-41 37.254805479625624 40.0 36.0 41.0 33.0 41.0 42-43 37.06098105198825 40.0 36.0 41.0 32.0 41.0 44-45 36.72635923894917 40.0 36.0 41.0 31.0 41.0 46-47 36.24426659138889 39.0 35.0 41.0 30.0 41.0 48-49 35.85437198027126 39.0 35.0 41.0 28.5 41.0 50-51 35.61059031562628 39.0 35.0 41.0 28.0 41.0 52-53 35.309193204876664 39.0 35.0 40.0 27.0 41.0 54-55 35.10112189968305 39.0 35.0 40.0 26.0 41.0 56-57 34.93357807926154 38.5 35.0 40.0 26.0 41.0 58-59 34.747578161247226 38.0 34.5 40.0 26.0 41.0 60-61 34.40713769310892 38.0 34.0 40.0 24.5 41.0 62-63 34.004995071541835 38.0 34.0 40.0 23.0 41.0 64-65 33.60411298001226 37.0 33.0 40.0 22.0 41.0 66-67 33.18726836712448 36.5 33.0 39.0 21.5 41.0 68-69 32.849376908729425 36.0 32.5 39.0 20.0 40.0 70-71 32.42669128100783 36.0 32.0 39.0 18.5 40.0 72-73 31.952321592609362 35.0 31.5 38.0 17.5 40.0 74-75 31.509488446538803 35.0 31.0 37.0 13.5 39.0 76-77 30.097581142545174 33.5 29.5 36.0 10.5 38.5 78-79 30.694640092122107 34.0 31.0 36.0 7.5 39.0 80-81 30.584070272919192 34.0 31.0 36.0 7.0 38.0 82-83 30.27172434546535 34.0 31.0 35.5 3.5 37.0 84-85 29.902659690429473 34.0 30.5 35.0 2.0 37.0 86-87 29.49071744935054 34.0 30.0 35.0 2.0 36.0 88-89 29.01100145524606 34.0 29.0 35.0 2.0 36.0 90-91 28.51184926184926 34.0 29.0 35.0 2.0 35.5 92-93 28.207004559522545 34.0 29.0 35.0 2.0 35.0 94-95 27.84282364318336 34.0 28.5 35.0 2.0 35.0 96-97 27.318134303745815 33.0 27.0 35.0 2.0 35.0 98-99 26.81935337510877 33.0 26.5 35.0 2.0 35.0 100-101 24.955156620624244 31.5 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12010125839720587 1101 2 0.2121272752919765 1101 3 0.13270185801338386 1101 4 0.05912433380073878 1101 5 0.02930367567154235 1101 6 0.07611450997134739 1101 7 0.08397284337261368 1101 8 0.04359140257316341 1101 9 0.08903225255762948 1101 10-11 0.10764256776316472 1101 12-13 0.09732723987026048 1101 14-15 0.06024945151190053 1101 16-17 0.07741595302830007 1101 18-19 0.0486808923983304 1101 20-21 0.17366968977400177 1101 22-23 0.08110142073806514 1101 24-25 0.1581760353692303 1101 26-27 0.17939649314761397 1101 28-29 0.17271085707594835 1101 30-31 0.21856417638353065 1101 32-33 0.2664290431557177 1101 34-35 0.18741984035211345 1101 36-37 0.17336903950062066 1101 38-39 0.13351113338909215 1101 40-41 0.14884345841319657 1101 42-43 0.37448182780104133 1101 44-45 0.337802835237639 1101 46-47 0.4428670380856019 1101 48-49 0.4228366423860592 1101 50-51 0.26423719513368127 1101 52-53 0.45753205557598875 1101 54-55 0.637665647838972 1101 56-57 0.5807569901753453 1101 58-59 0.46635924473940094 1101 60-61 0.49482621985539055 1101 62-63 0.615003270888316 1101 64-65 0.6937276813256901 1101 66-67 0.6999375135116566 1101 68-69 0.6700136415056974 1101 70-71 0.572893023829856 1101 72-73 0.5687487546975376 1101 74-75 0.555252659388433 1101 76-77 0.4862683779054109 1101 78-79 0.7200584963353407 1101 80-81 0.8630067431935053 1101 82-83 0.687190988590082 1101 84-85 0.6554878200874725 1101 86-87 0.8397376051429468 1101 88-89 0.8652624801794779 1101 90-91 0.837672937292794 1101 92-93 0.7776561143631469 1101 94-95 0.6318423029750235 1101 96-97 0.6257725905187321 1101 98-99 0.7274922312573793 1101 100-101 1.223937102083056 1102 1 0.1660434708986429 1102 2 0.11735347536104257 1102 3 0.2423714792865539 1102 4 0.16732048897513607 1102 5 0.0634466479289415 1102 6 0.1675499882722562 1102 7 0.1415646472174359 1102 8 0.11118665985415532 1102 9 0.13695443512929018 1102 10-11 0.23741694326678697 1102 12-13 0.1595524039084779 1102 14-15 0.26964855361848805 1102 16-17 0.1622150781578071 1102 18-19 0.21458396595651408 1102 20-21 0.20438800449714734 1102 22-23 0.16469759893537628 1102 24-25 0.14460403122510712 1102 26-27 0.2361306936771399 1102 28-29 0.2840553948910767 1102 30-31 0.47760642341933846 1102 32-33 0.3872014617121806 1102 34-35 0.30569427341301036 1102 36-37 0.24390547325220524 1102 38-39 0.13809269203995456 1102 40-41 0.3198115716859036 1102 42-43 0.23198729574164645 1102 44-45 0.3543505502140434 1102 46-47 0.47395947506038993 1102 48-49 0.6419974573969967 1102 50-51 0.6222894571343716 1102 52-53 0.8393900042349074 1102 54-55 0.7801429127175226 1102 56-57 0.7485421864456328 1102 58-59 0.7618294876310756 1102 60-61 0.7787619859426442 1102 62-63 0.7480814336602677 1102 64-65 0.8124510610885523 1102 66-67 0.8340632185882058 1102 68-69 0.7414364572073211 1102 70-71 0.8332130422481825 1102 72-73 0.7969921068329171 1102 74-75 0.6673799760685277 1102 76-77 0.798850397014423 1102 78-79 0.9470816343576729 1102 80-81 0.7927638515162876 1102 82-83 0.8912372646347464 1102 84-85 0.7957779087256682 1102 86-87 0.8695062249196255 1102 88-89 0.8972873449198921 1102 90-91 0.8235368719077343 1102 92-93 0.8594565057525827 1102 94-95 1.1146959923960011 1102 96-97 1.1022604459348706 1102 98-99 1.27052204592297 1102 100-101 1.703803569217854 1103 1 0.051843274419717034 1103 2 0.14036483297389424 1103 3 0.0956688621589663 1103 4 0.05050245814631893 1103 5 -0.04764734391505954 1103 6 -0.14268811874515563 1103 7 -0.01817625503075959 1103 8 0.05907309786081072 1103 9 -0.06345336466009144 1103 10-11 -0.028162139501731076 1103 12-13 -0.014473859246066922 1103 14-15 0.08737088224716416 1103 16-17 0.023570570733305374 1103 18-19 0.0760903515216782 1103 20-21 0.12049185562548814 1103 22-23 0.02349709855019455 1103 24-25 0.035213327929497495 1103 26-27 0.14941104366406677 1103 28-29 0.1245487831631209 1103 30-31 0.03233145893396028 1103 32-33 0.014089744359132794 1103 34-35 0.11661263571166813 1103 36-37 0.12483252868329231 1103 38-39 0.260995208807806 1103 40-41 0.33376581612547795 1103 42-43 0.43157278174428626 1103 44-45 0.24930323271681942 1103 46-47 0.4438812368685703 1103 48-49 0.36277086347935494 1103 50-51 0.4277915738767746 1103 52-53 0.42951163741299325 1103 54-55 0.2586108011344592 1103 56-57 0.22852571896713414 1103 58-59 0.3615125063389826 1103 60-61 0.23013592492110035 1103 62-63 0.2651273941807304 1103 64-65 0.39102814372984795 1103 66-67 0.6763998336379302 1103 68-69 0.5922442824400278 1103 70-71 0.30146049261605157 1103 72-73 0.5507767998522581 1103 74-75 0.5338050490413657 1103 76-77 0.5355036117065239 1103 78-79 0.5493959158314041 1103 80-81 0.5861860479810801 1103 82-83 0.597857120314611 1103 84-85 0.7690475866557129 1103 86-87 0.4993144812751531 1103 88-89 0.42075809570969724 1103 90-91 0.6749436751673059 1103 92-93 0.5710120110497385 1103 94-95 0.7706306454834717 1103 96-97 0.5546478769375511 1103 98-99 0.847096527692127 1103 100-101 1.0899249479627926 1104 1 0.1138248952021641 1104 2 0.13600864320751782 1104 3 0.12362212315180443 1104 4 0.09723410065448235 1104 5 0.026357066036638344 1104 6 -0.009253202356013901 1104 7 -0.036835329235486824 1104 8 0.11799470706603188 1104 9 0.10484064390352188 1104 10-11 -0.07092755039758458 1104 12-13 0.10480031042660443 1104 14-15 0.07792240606665501 1104 16-17 0.160709537237409 1104 18-19 -0.0989953361671283 1104 20-21 -0.2269789030364393 1104 22-23 -0.05867802101488451 1104 24-25 -0.05998985536630386 1104 26-27 -8.98540866245412E-5 1104 28-29 -0.02055173692837542 1104 30-31 -0.03398094357650194 1104 32-33 -0.03813298029579926 1104 34-35 0.08348129014614614 1104 36-37 -0.0014807723143164253 1104 38-39 0.017945964347127585 1104 40-41 0.048705300806275886 1104 42-43 0.002777888686360086 1104 44-45 -0.02503693157376574 1104 46-47 0.23348205817419654 1104 48-49 0.16299555891430373 1104 50-51 -0.13417568584050343 1104 52-53 0.08275414498752554 1104 54-55 0.26142078288485493 1104 56-57 0.23025862083024862 1104 58-59 0.45194266074621225 1104 60-61 0.34821333916344344 1104 62-63 0.5750053030328885 1104 64-65 0.2433190650326864 1104 66-67 0.4744618999521677 1104 68-69 0.42909337636728395 1104 70-71 0.45345690161330054 1104 72-73 0.4576751190921158 1104 74-75 0.29357241451500826 1104 76-77 0.4037297918546727 1104 78-79 0.40660936839349304 1104 80-81 0.1637722548776317 1104 82-83 0.4233812255466063 1104 84-85 0.3158716555063421 1104 86-87 0.38957348124422 1104 88-89 0.55930371013498 1104 90-91 0.3554887770130364 1104 92-93 0.5388748930724816 1104 94-95 0.919864574715092 1104 96-97 0.7687713585310867 1104 98-99 0.8648817863164986 1104 100-101 1.15452101906995 1105 1 0.1649208343440236 1105 2 0.27247427368037336 1105 3 0.2049863689924365 1105 4 0.14599039443731954 1105 5 0.023343575449949583 1105 6 0.08265141946973387 1105 7 0.1457630453266816 1105 8 0.04979780743818907 1105 9 0.22260963470912287 1105 10-11 0.24434479253149277 1105 12-13 0.19738476341478162 1105 14-15 0.17455762662269336 1105 16-17 0.24686253088889742 1105 18-19 0.2355423102317289 1105 20-21 0.14343245040051045 1105 22-23 0.0906667756685664 1105 24-25 0.2121033747125054 1105 26-27 0.2862242467233571 1105 28-29 0.30313939567889037 1105 30-31 0.17418763402857707 1105 32-33 0.1537521444852885 1105 34-35 0.18040212530376465 1105 36-37 0.18931234693918242 1105 38-39 0.021648692565165106 1105 40-41 0.12225790178044349 1105 42-43 0.1274811573487895 1105 44-45 0.3249469919157235 1105 46-47 0.17448468514442794 1105 48-49 0.3782574692142191 1105 50-51 0.40566327541168334 1105 52-53 0.25019784299541215 1105 54-55 0.1157103037364351 1105 56-57 0.15798529962840746 1105 58-59 0.13666946268359936 1105 60-61 0.1830391655644945 1105 62-63 0.17281718733674012 1105 64-65 0.2285380818343512 1105 66-67 0.16740995572055795 1105 68-69 0.3589595992766519 1105 70-71 0.12215080485322716 1105 72-73 0.2388866380829171 1105 74-75 0.13396430511264512 1105 76-77 0.1320453156068595 1105 78-79 0.1660064308387632 1105 80-81 0.2584988173776317 1105 82-83 -0.017564107476598423 1105 84-85 0.06055272490213781 1105 86-87 0.23696207444693584 1105 88-89 0.07992012459426334 1105 90-91 0.15172670252748333 1105 92-93 0.21839820743325333 1105 94-95 0.3387645651375948 1105 96-97 0.3263979524999989 1105 98-99 0.09797494114355842 1105 100-101 0.4531404914867636 1106 1 -0.1008078714081897 1106 2 -0.03407992985945185 1106 3 -7.831276889760375E-4 1106 4 0.05400339222492789 1106 5 0.02367197699862089 1106 6 -0.3420841308621263 1106 7 -0.10570611949632536 1106 8 -0.05189432475207667 1106 9 -0.2839238276802547 1106 10-11 -0.2622837334640806 1106 12-13 -0.3590614132998198 1106 14-15 -0.3899214248153626 1106 16-17 -0.14543904543853614 1106 18-19 -0.22325625862888643 1106 20-21 -0.06963965966586017 1106 22-23 -0.031978239541615494 1106 24-25 0.006610426703652195 1106 26-27 0.011170665418049452 1106 28-29 -0.003401844641906848 1106 30-31 0.010756010687863693 1106 32-33 0.07455551837909269 1106 34-35 -0.060973012970571006 1106 36-37 0.040110017016615984 1106 38-39 0.1911816709943679 1106 40-41 0.05177687993310087 1106 42-43 -0.4236068888348399 1106 44-45 -0.015406644699325511 1106 46-47 0.034551056825840476 1106 48-49 0.20768311218988345 1106 50-51 0.2718655707656694 1106 52-53 0.38498939443346814 1106 54-55 0.21478818675634415 1106 56-57 0.11947157590053337 1106 58-59 0.01803440322563432 1106 60-61 0.22899809808662042 1106 62-63 0.09713791465974708 1106 64-65 0.15476440235426026 1106 66-67 0.16916863243515934 1106 68-69 0.3217508395974491 1106 70-71 0.2756007495434929 1106 72-73 0.42641688559398006 1106 74-75 0.27170714941574303 1106 76-77 0.36618697766438046 1106 78-79 0.33743636031885416 1106 80-81 0.3363386265590478 1106 82-83 0.33029523295481766 1106 84-85 0.17271049591983711 1106 86-87 0.47052039164826454 1106 88-89 0.5818257177911654 1106 90-91 0.40239646884385394 1106 92-93 0.33489433575183725 1106 94-95 -0.13176139586904156 1106 96-97 0.22079524923672622 1106 98-99 0.3993481781457717 1106 100-101 0.5468559229026937 1107 1 0.09064428533046254 1107 2 -0.05696083729372958 1107 3 -0.043383217166628185 1107 4 -0.03221841413234472 1107 5 -0.03691912106669548 1107 6 -0.06538269158144061 1107 7 -0.06460368941437622 1107 8 0.060648368088621396 1107 9 0.10123652281023254 1107 10-11 -0.0013841011953843463 1107 12-13 -0.03296176575445742 1107 14-15 -0.09591165320159689 1107 16-17 -0.0659144227177606 1107 18-19 0.037622231817735496 1107 20-21 0.18502245309780108 1107 22-23 -0.03730325072631757 1107 24-25 0.010396858837047773 1107 26-27 -0.08207227184939114 1107 28-29 -0.16184343319749672 1107 30-31 -0.3567986579720426 1107 32-33 -0.19751654386242734 1107 34-35 -0.0883223467985772 1107 36-37 -0.40770992013079166 1107 38-39 -0.2229627535661507 1107 40-41 -0.25637199668595656 1107 42-43 -0.4592492728685329 1107 44-45 -0.5255233475571544 1107 46-47 -0.5755923802069915 1107 48-49 -0.586928860355755 1107 50-51 -0.5192341313392461 1107 52-53 -0.4866251860582267 1107 54-55 -0.3746499045326743 1107 56-57 -0.394541360166599 1107 58-59 -0.3007372159924131 1107 60-61 -0.47760115117409896 1107 62-63 -0.5380613095035827 1107 64-65 -0.385737190784333 1107 66-67 -0.4939377968611325 1107 68-69 -0.46956096272241865 1107 70-71 -0.5880979235684762 1107 72-73 -0.5294567217737409 1107 74-75 -0.5810823435078838 1107 76-77 -0.6816985673308729 1107 78-79 -0.8081748702170302 1107 80-81 -0.7902967956772393 1107 82-83 -0.6262569587019371 1107 84-85 -0.5858642179153719 1107 86-87 -0.3769495460447416 1107 88-89 -0.7036400297291046 1107 90-91 -0.48312261062757145 1107 92-93 -0.3538987253657595 1107 94-95 -0.6257618923608632 1107 96-97 -0.5243728214287948 1107 98-99 -0.3696317221193901 1107 100-101 0.2737949689836796 1108 1 -1.5337097750034445E-5 1108 2 0.03596202783933933 1108 3 -0.021377569316250344 1108 4 -0.021992796412959592 1108 5 -0.05189686470716737 1108 6 -0.08226128559034152 1108 7 0.006029767831300603 1108 8 -0.09399515409138814 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-0.35002012542572913 2316 70-71 -0.22094195308327613 2316 72-73 -0.1302565704157601 2316 74-75 -0.16070903731460362 2316 76-77 0.11659082651955543 2316 78-79 -0.22735036066255887 2316 80-81 -0.08887324776786798 2316 82-83 -0.3511997101551678 2316 84-85 -0.4964723247010383 2316 86-87 -0.5561084654009463 2316 88-89 -0.49620783092822407 2316 90-91 -0.2880410926114081 2316 92-93 -0.22289643542389115 2316 94-95 -0.11329965030552813 2316 96-97 -0.3525325823875676 2316 98-99 -0.8109283119979764 2316 100-101 -1.2574787214761969 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 6.0 5 17.0 6 54.0 7 145.0 8 315.0 9 632.0 10 1078.0 11 1810.0 12 2821.0 13 4097.0 14 5319.0 15 7658.0 16 10754.0 17 15682.0 18 13601.0 19 7218.0 20 5792.0 21 5555.0 22 5766.0 23 6136.0 24 7017.0 25 8148.0 26 9709.0 27 12078.0 28 15053.0 29 18929.0 30 23776.0 31 29765.0 32 38180.0 33 50256.0 34 69323.0 35 99126.0 36 146272.0 37 220364.0 38 207189.0 39 23707.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.29068894898204 20.08083677998917 15.054638467009514 19.57383580401928 2 14.34740319632406 23.377288844914744 40.04432817382458 22.23097978493662 3 21.284976680660133 28.019356076910036 30.5779618729259 20.11770536950393 4 11.767835149130113 17.703878854957992 39.577009720894615 30.951276275017282 5 11.732131442660162 41.03175742034555 33.62799781587368 13.608113321120612 6 27.586788378155287 38.36809340406463 18.470910916954082 15.574207300826007 7 25.572362622722334 36.62757439735857 20.650426046109498 17.149636933809596 8 24.946103723082143 34.22353026669573 20.378941601963184 20.45142440825894 9 26.77335986688505 18.312995291412555 21.582920144071224 33.33072469763117 10-11 23.31458842250209 27.959590369662312 27.242401882689656 21.483419325145945 12-13 27.76422699843198 25.58204427533403 27.38102669358603 19.27270203264796 14-15 23.99250762560115 26.37009273699921 28.824539436050227 20.81286020134941 16-17 19.364089148261808 30.2714005951416 29.903396630015333 20.461113626581255 18-19 19.08962340617017 29.555842505482794 32.743036340158646 18.611497748188395 20-21 21.082388168719106 27.753368400850416 31.507754169624675 19.656489260805806 22-23 20.061918322037698 27.68436562368548 30.836088338420993 21.417627715855833 24-25 20.410310828550056 27.799604418660333 31.9415627792058 19.848521973583814 26-27 21.492556920135726 26.670197680896933 32.65228546992303 19.184959929044314 28-29 19.79504933936999 27.260636625311008 33.634296580320246 19.31001745499876 30-31 19.860521838939103 28.95650845291133 32.420869830941776 18.762099877207792 32-33 19.42343561048597 28.791186165286888 31.80341507679637 19.981963147430772 34-35 20.25149111480047 27.354107390078614 31.992587747983432 20.40181374713749 36-37 21.06976423523186 27.54309373014409 32.39315307660631 18.993988958017734 38-39 21.78126962299624 26.486875767451306 32.910874097924456 18.820980511627994 40-41 20.293928027740975 27.57667991480941 33.28102087094893 18.848371186500685 42-43 19.375488314690603 29.028989295163605 31.97357638068722 19.621946009458572 44-45 20.190654003603644 29.026382623504926 30.556859873406633 20.226103499484793 46-47 20.119475515158967 28.71879652455192 29.422196508527442 21.739531451761668 48-49 21.009951945203746 28.31995475880368 29.9943262173478 20.67576707864478 50-51 21.933129487086322 29.193009228980454 29.65562282109045 19.21823846284278 52-53 20.515382565742275 29.605592914945433 30.118981737686774 19.760042781625515 54-55 19.354586261061083 28.987066756850933 30.826248092435144 20.832098889652848 56-57 20.89652287494014 30.240562794519626 28.389456267154106 20.473458063386122 58-59 20.7976742149404 29.132917442269964 29.08246829110138 20.98694005168825 60-61 20.561080273310488 28.397002677578215 30.11609360530224 20.925823443809055 62-63 22.454018137471373 28.105161558398965 29.541727923022886 19.89909238110677 64-65 21.64995276506068 28.636577917153456 30.147816478751732 19.565652839034133 66-67 20.213805645460322 28.95119801594622 30.3216168324082 20.513379506185263 68-69 20.192237818137098 30.063035818431498 29.74659899120331 19.99812737222809 70-71 20.31167606707175 30.173716504651754 28.472047536795735 21.042559891480757 72-73 20.4603312503057 28.855437261117565 28.420586803557995 22.26364468501874 74-75 20.74401085192452 29.71981389247576 28.66368909534377 20.872486160255942 76-77 21.405486333543887 28.339426361009096 29.519601102334914 20.735486203112103 78-79 20.4431326733485 28.085503625071972 30.338386633350662 21.13297706822887 80-81 20.087286813905518 30.215734172568702 29.216999360884255 20.47997965264152 82-83 20.023803811870696 29.93485395126973 28.86456749032825 21.17677474653133 84-85 20.310114786072877 28.479439732536942 28.750135674054324 22.460309807335854 86-87 20.8249717242523 28.796403436691207 29.058245245295605 21.320379593760887 88-89 21.74377048477768 27.531075372801993 29.62967621241003 21.095477930010304 90-91 20.202756655675202 28.033406723645875 30.33793397108055 21.42590264959837 92-93 19.46247478676474 29.348531925895216 29.8792093874756 21.309783899864442 94-95 19.475282245066417 29.751164103682086 28.835579555004013 21.93797409624748 96-97 19.261700196951995 28.91025175197837 28.389130187691336 23.438917863378293 98-99 20.110969861308636 28.243557929835855 28.522869191865315 23.122603016990194 100-101 20.60604027658589 28.88810309822042 28.716424919565164 21.78943170562853 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1362.0 1 957.5 2 658.5 3 796.0 4 686.0 5 665.0 6 1226.0 7 2279.5 8 3237.0 9 3533.5 10 3069.0 11 2526.0 12 2446.0 13 2498.5 14 2536.0 15 2570.0 16 2585.5 17 2704.5 18 2871.0 19 3083.0 20 3418.0 21 3841.5 22 4303.0 23 4911.0 24 5716.0 25 6841.5 26 8340.0 27 10176.5 28 12045.0 29 14264.0 30 16839.0 31 19199.5 32 21733.0 33 24786.5 34 28216.0 35 30670.5 36 32324.5 37 34250.0 38 34542.5 39 33946.5 40 33485.0 41 33002.0 42 33132.0 43 33730.5 44 35275.5 45 35550.5 46 36035.0 47 44097.5 48 84029.5 49 80976.5 50 41624.0 51 32550.5 52 24278.0 53 20740.0 54 18325.0 55 16079.5 56 14201.0 57 12368.5 58 10796.0 59 9346.5 60 8103.5 61 6833.5 62 5295.5 63 3511.5 64 2153.5 65 1569.0 66 1074.0 67 737.0 68 530.0 69 365.5 70 297.5 71 242.0 72 191.5 73 222.0 74 195.0 75 140.0 76 112.5 77 72.5 78 49.0 79 28.0 80 12.5 81 6.5 82 3.0 83 3.0 84 3.0 85 1.0 86 1.0 87 3.0 88 2.5 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05394285969825538 2 0.0 3 0.0 4 0.0 5 0.014906489726633613 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0027018012629523424 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.919072667274106E-4 24-25 9.316556079146007E-5 26-27 0.0011179867294975208 28-29 0.0024688873609736922 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0141145824599062 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 4.6582780395730035E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.6582780395730035E-5 84-85 0.001257735070684711 86-87 0.0 88-89 0.0 90-91 0.009409721639937467 92-93 0.0012111522902889808 94-95 0.0 96-97 0.0 98-99 3.2607946277011027E-4 100-101 0.01201835734209835 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1073358.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.36929051711541 #Duplication Level Percentage of deduplicated Percentage of total 1 77.24314360093348 41.99654914894417 2 13.537898883769486 14.720919148059913 3 4.429845335000898 7.225426438936569 4 1.906295867901728 4.145758154141029 5 0.9548793617877168 2.595805671491706 6 0.5399594900549933 1.7614328629364056 7 0.31022245862546377 1.1806602484559159 8 0.22125789773399196 0.9623707936884496 9 0.14997178002905034 0.7338473349991529 >10 0.6228259923402586 6.025425428567028 >50 0.04185496937311211 1.5697362400902506 >100 0.0303543129550962 3.247817864524834 >500 0.005144798281303494 1.8902479813367796 >1k 0.005316291557346944 6.566963723846038 >5k 6.859731041737992E-4 2.796537646339172 >10k+ 3.429865520868996E-4 2.580501313642614 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 13842 1.2895976924753905 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 13834 1.2888523679890587 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9773 0.9105070256149393 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7785 0.7252938907615166 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 7053 0.6570967002621678 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5382 0.5014170481796381 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 4883 0.45492743334469954 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 4870 0.4537162810544106 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 4543 0.4232511426756031 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4333 0.40368637490939646 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 4032 0.375643541111167 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 3657 0.3407064558143695 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 3581 0.3336258731942185 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 2931 0.27306825867976947 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 2859 0.2663603383027843 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2787 0.25965241792579924 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 2373 0.22108187575813476 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2107 0.19629983658760639 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2068 0.19266637971673942 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2048 0.19080306850091022 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1973 0.1838156514415507 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1804 0.168070671667794 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1752 0.16322606250663807 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1651 0.15381634086670057 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1598 0.1488785661447532 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1568 0.1460835993210094 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1323 0.12325803692710167 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1291 0.12027673898177495 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1284 0.11962458005623473 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1276 0.11887925556990306 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1210 0.11273032855766668 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1163 0.10835154720046808 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1154 0.10751305715334492 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1132 0.1054634148159328 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1105 0.10294794467456338 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 4.6582780395730035E-5 0.0 9.316556079146007E-5 0.0 20-21 0.0 9.316556079146007E-5 0.0 3.726622431658403E-4 0.0 22-23 0.0 1.8633112158292014E-4 0.0 6.987417059359505E-4 0.0 24-25 0.0 1.8633112158292014E-4 0.0 0.0011645695098932507 0.0 26-27 0.0 1.8633112158292014E-4 0.0 0.0020962251178078518 0.0 28-29 0.0 1.8633112158292014E-4 0.0 0.005077523063134574 0.0 30-31 0.0 1.8633112158292014E-4 0.0 0.010481125589039257 0.0 32-33 0.0 1.8633112158292014E-4 0.0 0.019098939962249317 0.0 34-35 0.0 1.8633112158292014E-4 0.0 0.02706459540991915 0.0 36-37 0.0 1.8633112158292014E-4 0.0 0.03652089983025235 0.0 38-39 0.0 1.8633112158292014E-4 0.0 0.045977204250585546 0.0 40-41 0.0 1.8633112158292014E-4 0.0 0.061023442318406354 0.0 42-43 0.0 1.8633112158292014E-4 0.0 0.0790975611119496 0.0 44-45 0.0 1.8633112158292014E-4 0.0 0.09731142824668004 0.0 46-47 0.0 2.794966823743802E-4 0.0 0.1186463416679244 0.0 48-49 0.0 2.794966823743802E-4 0.0 0.13844402333610967 0.0 50-51 0.0 2.794966823743802E-4 0.0 0.1671855988402751 0.0 52-53 0.0 2.794966823743802E-4 0.0 0.24120563688909014 0.0 54-55 0.0 2.794966823743802E-4 0.0 0.3409859524967439 0.0 56-57 0.0 2.794966823743802E-4 0.0 0.40997505026282005 0.0 58-59 0.0 2.794966823743802E-4 0.0 0.5402205042492813 0.0 60-61 0.0 2.794966823743802E-4 0.0 0.7200766193571949 0.0 62-63 0.0 2.794966823743802E-4 0.0 0.8153849880468585 0.0 64-65 0.0 3.726622431658403E-4 0.0 0.8915012512134814 0.0 66-67 0.0 3.726622431658403E-4 0.0 1.1111856435597443 0.0 68-69 0.0 3.726622431658403E-4 0.0 1.308603466876848 0.0 70-71 0.0 3.726622431658403E-4 0.0 1.4048434911744265 0.0 72-73 0.0 3.726622431658403E-4 0.0 1.6343102674037926 0.0 74-75 0.0 4.192450235615703E-4 0.0 1.9535420614557304 0.0 76-77 0.0 4.6582780395730035E-4 0.0 2.2458490084389364 0.0 78-79 0.0 4.6582780395730035E-4 0.0 2.538482034884913 0.0 80-81 0.0 4.6582780395730035E-4 0.0 2.9455223699828017 0.0 82-83 0.0 4.6582780395730035E-4 0.0 3.3690995921211746 0.0 84-85 0.0 4.6582780395730035E-4 0.0 3.867908004598652 0.0 86-87 0.0 4.6582780395730035E-4 0.0 4.557472902796643 0.0 88-89 0.0 5.589933647487604E-4 0.0 5.230920158977713 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACCGT 20 5.3892325E-4 47.499996 18-19 TCGACCG 20 5.3892325E-4 47.499996 18-19 TATCACG 50 0.001615399 38.00177 2 CTACGGT 55 0.0025789605 34.547066 3 CGGGAGT 190 3.947207E-10 27.501282 4 CGTATAG 70 0.008368468 27.15804 1 GTTCGAC 35 0.0083941175 27.142227 16-17 TGAGCCG 90 8.9447264E-4 26.39012 5 GTATAGG 355 0.0 22.759201 1 GAGTACT 7235 0.0 22.520054 12-13 CATTCCG 150 3.0318815E-5 22.1677 9 AGTACTT 7410 0.0 21.859993 12-13 GTATAGC 485 0.0 21.558443 1 CTTACAC 445 0.0 21.34931 3 GTACTTT 7795 0.0 20.991646 14-15 GTACACG 115 0.0037110765 20.663727 1 AGCCCGT 115 0.0037241247 20.651213 6 CATGGGT 1050 0.0 19.90569 4 TTCGACC 60 0.0047654626 19.791206 16-17 GCTCCGC 60 0.0047661047 19.790745 34-35 >>END_MODULE