##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937380_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1117216 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.848503780826626 33.0 31.0 34.0 30.0 34.0 2 31.914798033683727 33.0 31.0 34.0 30.0 34.0 3 31.957752126714976 33.0 31.0 34.0 30.0 34.0 4 35.55359303840976 37.0 35.0 37.0 33.0 37.0 5 35.567251095580446 37.0 35.0 37.0 33.0 37.0 6 35.624480852404545 37.0 35.0 37.0 33.0 37.0 7 35.53197233122333 37.0 35.0 37.0 33.0 37.0 8 35.564937308452436 37.0 35.0 37.0 33.0 37.0 9 37.1762022742245 39.0 37.0 39.0 34.0 39.0 10-11 37.06953131713115 39.0 37.0 39.0 33.0 39.0 12-13 36.9348948636611 39.0 37.0 39.0 33.0 39.0 14-15 38.02662869131842 40.0 38.0 41.0 33.0 41.0 16-17 37.99446928794432 40.0 38.0 41.0 33.0 41.0 18-19 38.06193072780913 40.0 38.0 41.0 33.0 41.0 20-21 38.00161875590754 40.0 38.0 41.0 33.0 41.0 22-23 37.87146129307135 40.0 37.5 41.0 33.0 41.0 24-25 37.80997810629279 40.0 37.5 41.0 33.0 41.0 26-27 37.784621774124254 40.0 37.5 41.0 33.0 41.0 28-29 37.70729026437144 40.0 37.0 41.0 33.0 41.0 30-31 37.50823744020852 40.0 37.0 41.0 33.0 41.0 32-33 37.26803903631885 39.0 36.0 41.0 32.0 41.0 34-35 37.288545366339186 40.0 36.0 41.0 32.0 41.0 36-37 37.10851035072895 39.5 36.0 41.0 31.5 41.0 38-39 36.94266730873886 39.0 36.0 41.0 31.0 41.0 40-41 36.698677784779306 39.0 35.5 41.0 30.5 41.0 42-43 36.46490427992438 39.0 35.0 41.0 30.0 41.0 44-45 36.21596271446166 39.0 35.0 41.0 30.0 41.0 46-47 35.77163950390972 39.0 35.0 41.0 28.5 41.0 48-49 35.38096035144502 39.0 35.0 40.5 26.5 41.0 50-51 34.38082116618452 38.0 34.0 39.5 25.0 40.5 52-53 34.44665892719044 38.0 34.5 39.5 24.0 40.5 54-55 34.846678708503994 39.0 35.0 40.0 24.5 41.0 56-57 34.699805588176325 38.5 34.5 40.0 23.5 41.0 58-59 34.4919004024289 38.0 34.0 40.0 23.0 41.0 60-61 34.15925702818434 38.0 34.0 40.0 22.0 41.0 62-63 33.81989203520179 38.0 34.0 40.0 21.5 41.0 64-65 33.36151961661845 37.0 33.0 40.0 19.0 41.0 66-67 33.028865948930196 37.0 33.0 39.5 18.0 41.0 68-69 32.60580720290436 36.0 32.0 39.0 15.0 41.0 70-71 32.11655355813021 36.0 31.5 39.0 10.5 40.0 72-73 31.60492062412282 35.0 31.0 38.0 7.5 40.0 74-75 31.12281152436055 35.0 31.0 37.5 4.5 39.5 76-77 30.58240080700599 35.0 30.0 37.0 2.0 39.0 78-79 30.081650280697737 34.0 29.5 36.0 2.0 39.0 80-81 29.601041786010942 34.0 29.0 36.0 2.0 38.0 82-83 29.122025642310888 34.0 29.0 35.5 2.0 37.0 84-85 28.59856061853751 34.0 28.5 35.0 2.0 37.0 86-87 28.077960304900756 34.0 27.0 35.0 2.0 36.0 88-89 27.54424569644545 33.5 26.0 35.0 2.0 36.0 90-91 26.990277171111046 33.0 25.5 35.0 2.0 35.5 92-93 26.54120420760175 33.0 25.0 35.0 2.0 35.0 94-95 26.05392511385444 32.5 24.5 35.0 2.0 35.0 96-97 25.284028334717725 32.0 19.5 34.5 2.0 35.0 98-99 24.35593161931086 32.0 6.5 34.0 2.0 35.0 100-101 21.843605444247128 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13608072379326686 1101 2 0.13013273084381538 1101 3 0.10596654718492005 1101 4 0.16355190744329207 1101 5 0.002747111321468765 1101 6 0.009039860110625852 1101 7 0.08294297287980612 1101 8 -0.022810829545313993 1101 9 0.09504729291933245 1101 10-11 0.04858280214416055 1101 12-13 0.15067964536973477 1101 14-15 0.21272576186633074 1101 16-17 0.12431236473189955 1101 18-19 0.1719343008241907 1101 20-21 0.1165833621831851 1101 22-23 0.23961548284852796 1101 24-25 0.3367948956730302 1101 26-27 0.2838566384158696 1101 28-29 0.05734537517296445 1101 30-31 0.1410555849724915 1101 32-33 -0.015703195348301335 1101 34-35 0.12245836048330716 1101 36-37 0.10288169985691553 1101 38-39 0.036230359630756936 1101 40-41 0.02963904010293561 1101 42-43 0.08944130557260621 1101 44-45 0.15740087855181883 1101 46-47 0.14919910854696639 1101 48-49 0.12764986071864115 1101 50-51 0.11370568689058302 1101 52-53 0.1482300042318201 1101 54-55 0.12974107811175628 1101 56-57 0.27204801626099595 1101 58-59 0.10941698312911541 1101 60-61 0.2741729171220939 1101 62-63 0.2054097360625562 1101 64-65 0.06736666249471313 1101 66-67 -0.0725535068793377 1101 68-69 -0.06442708054240853 1101 70-71 -0.18808626572445064 1101 72-73 -0.1170118224463117 1101 74-75 0.055185922542886345 1101 76-77 0.1062169676844249 1101 78-79 -0.07215020532244054 1101 80-81 0.05982867915685475 1101 82-83 0.27184348150083437 1101 84-85 0.07169513161269236 1101 86-87 0.010431053381289246 1101 88-89 -0.04632690730830902 1101 90-91 0.008139652695504651 1101 92-93 0.18076802728245056 1101 94-95 0.18260058631035392 1101 96-97 0.4710061508529755 1101 98-99 0.6855358370559195 1101 100-101 0.9707784291127837 1102 1 -0.02522519091922959 1102 2 -0.17572927927504267 1102 3 0.09562935235678083 1102 4 -0.08356401770929267 1102 5 0.08512444026460741 1102 6 -0.01409397381163302 1102 7 0.13646449552677353 1102 8 -0.01009901618199649 1102 9 0.011172714538346895 1102 10-11 -0.049216246201481795 1102 12-13 0.027191199738510363 1102 14-15 0.20711697412139074 1102 16-17 0.1724738046612302 1102 18-19 0.26860702408149706 1102 20-21 0.11571101235985992 1102 22-23 -0.026990785220853297 1102 24-25 0.1779795447253889 1102 26-27 0.033494246832191266 1102 28-29 0.03028722186426336 1102 30-31 0.05977973571132367 1102 32-33 0.011164978439033746 1102 34-35 0.1450681821986919 1102 36-37 0.004857286108112646 1102 38-39 0.13266667508208485 1102 40-41 0.24156910153884326 1102 42-43 0.14145303059670056 1102 44-45 0.39317048383611564 1102 46-47 0.39694921897099533 1102 48-49 0.1454037857909114 1102 50-51 0.015552026835969457 1102 52-53 0.11029935052157214 1102 54-55 0.2613336435630842 1102 56-57 0.19013647594618988 1102 58-59 -0.11918158537392998 1102 60-61 -0.022380096450028475 1102 62-63 0.10575456018720075 1102 64-65 0.14160323091746108 1102 66-67 0.18888943401368863 1102 68-69 0.04259563669177879 1102 70-71 -0.1945026546579527 1102 72-73 -0.13549529899626478 1102 74-75 -0.1299888987709572 1102 76-77 -0.05189719068370735 1102 78-79 0.034856701198602735 1102 80-81 0.028328468347346814 1102 82-83 0.008955993547090202 1102 84-85 0.13828533800524312 1102 86-87 0.26914325824797913 1102 88-89 -0.04614947597197627 1102 90-91 0.09706211495056394 1102 92-93 0.4014456794668355 1102 94-95 0.40983014782657534 1102 96-97 0.3975598671234515 1102 98-99 0.2644568939142573 1102 100-101 0.5105604420424328 1103 1 -0.10173521552837883 1103 2 -0.02682131025361656 1103 3 -0.14075871220604697 1103 4 0.012239760518845344 1103 5 -0.04855209418819584 1103 6 -0.16487024769698166 1103 7 -0.18457632670831714 1103 8 -0.09084778572455576 1103 9 -0.1802222261101747 1103 10-11 0.02799948193980839 1103 12-13 -0.09711440608083421 1103 14-15 -0.14244377066726344 1103 16-17 -0.09947086094386748 1103 18-19 -0.2045083855884542 1103 20-21 -0.14613214050638135 1103 22-23 -0.20541163785548378 1103 24-25 -0.40232137449869754 1103 26-27 -0.42344879022629556 1103 28-29 -0.31351027741194315 1103 30-31 -0.45435921550465963 1103 32-33 -0.39462360002071506 1103 34-35 -0.17938354044171945 1103 36-37 -0.2926962749804787 1103 38-39 -0.38410735502792903 1103 40-41 -0.19986429090469926 1103 42-43 0.07055696806051515 1103 44-45 -0.12408080090621354 1103 46-47 0.17454177470347787 1103 48-49 0.3127165934052769 1103 50-51 0.07345816826619966 1103 52-53 0.05785110211392919 1103 54-55 0.12978552905816088 1103 56-57 0.10960484595197784 1103 58-59 -0.1175869668157361 1103 60-61 0.1169940892766661 1103 62-63 0.20358949794052705 1103 64-65 -0.10364231772179977 1103 66-67 0.2603916777890234 1103 68-69 0.264211426355935 1103 70-71 0.1454044277103108 1103 72-73 0.10746123690413967 1103 74-75 0.12042049975249469 1103 76-77 0.27639066397755485 1103 78-79 0.10839462823971147 1103 80-81 0.08974050873530004 1103 82-83 0.1785600757706014 1103 84-85 0.22384284379562658 1103 86-87 0.1930878695681848 1103 88-89 0.24879614866612698 1103 90-91 0.2582248972319796 1103 92-93 0.06487259391385436 1103 94-95 -0.02436752154227051 1103 96-97 0.024683763330749287 1103 98-99 0.40655663334679915 1103 100-101 1.2359716500621225 1104 1 0.02089036380203879 1104 2 -0.025703067825329384 1104 3 -0.10026460687697636 1104 4 0.021566787726207792 1104 5 0.06166118665681353 1104 6 0.11464545004010063 1104 7 0.11786237846575176 1104 8 0.01900942825430718 1104 9 0.12674975559760782 1104 10-11 0.0416201623033956 1104 12-13 0.1447303795604995 1104 14-15 0.13201802303722587 1104 16-17 -0.01656855029444415 1104 18-19 0.14667170340901947 1104 20-21 0.0566986231576081 1104 22-23 -0.09811938843673573 1104 24-25 0.02903727401261591 1104 26-27 0.07005635766655871 1104 28-29 0.07127065485452277 1104 30-31 -0.010887877925036094 1104 32-33 0.06245815293175383 1104 34-35 0.17440096917400894 1104 36-37 0.06769151070704282 1104 38-39 0.06611706333203671 1104 40-41 0.21597144614730013 1104 42-43 -0.012670293701887658 1104 44-45 0.07217780243914973 1104 46-47 0.09633794278244068 1104 48-49 0.017608374740440524 1104 50-51 -0.07846209071951904 1104 52-53 -0.16260304673870252 1104 54-55 -0.26301524800162923 1104 56-57 -0.16891803471237665 1104 58-59 -0.015288956170941503 1104 60-61 0.027526675489760066 1104 62-63 0.10356550352468474 1104 64-65 0.22666968667162024 1104 66-67 0.10943440324670917 1104 68-69 -0.14365931944696797 1104 70-71 -0.16615279704679153 1104 72-73 -0.08890129947242187 1104 74-75 0.04645450510055227 1104 76-77 -0.04270715881791176 1104 78-79 -0.038210290230598076 1104 80-81 0.011503636375330473 1104 82-83 0.090902828943765 1104 84-85 0.19441504004172216 1104 86-87 0.4331224882494418 1104 88-89 0.19155374860864072 1104 90-91 0.3276736543982217 1104 92-93 0.3379867868032953 1104 94-95 0.5788161653344481 1104 96-97 0.76181403119568 1104 98-99 0.6101389868012248 1104 100-101 0.8978274577372538 1105 1 0.0928008906798965 1105 2 0.07808101058569861 1105 3 0.02342943254767249 1105 4 0.14626357186980243 1105 5 0.05964182799736051 1105 6 0.06069625854637195 1105 7 0.15395980142569243 1105 8 0.03217478682222463 1105 9 0.11642203976131782 1105 10-11 2.8329416779371286E-4 1105 12-13 0.16462751154417532 1105 14-15 0.0943356947832612 1105 16-17 0.07080373151148933 1105 18-19 0.12191514292048566 1105 20-21 0.14575081442526283 1105 22-23 0.1293313376863594 1105 24-25 0.17465577888279427 1105 26-27 -0.003836080972540401 1105 28-29 0.002051103690270395 1105 30-31 0.11819990176515205 1105 32-33 0.19527397937786617 1105 34-35 0.19470585627161086 1105 36-37 0.12314863331512527 1105 38-39 0.3285133735643271 1105 40-41 0.33300444518616246 1105 42-43 0.26792971656134057 1105 44-45 0.23704089241849857 1105 46-47 0.38315968583846427 1105 48-49 0.3818758509997977 1105 50-51 0.2649609857234694 1105 52-53 0.18605774122725194 1105 54-55 0.38674009893001227 1105 56-57 0.3107919876032028 1105 58-59 0.32919858302694394 1105 60-61 0.3755005902578503 1105 62-63 0.42374445809050343 1105 64-65 0.308619102846734 1105 66-67 0.38782886055101073 1105 68-69 0.32222898339026074 1105 70-71 0.09477927011907639 1105 72-73 0.31595376022764654 1105 74-75 0.3843947315294649 1105 76-77 0.3150861648162113 1105 78-79 0.37448469368803927 1105 80-81 0.6458710819837101 1105 82-83 0.5955670481551358 1105 84-85 0.39398793736371474 1105 86-87 0.4172415360996453 1105 88-89 0.5298601224867951 1105 90-91 0.8005929591064067 1105 92-93 1.0206593441906655 1105 94-95 1.0871522609544115 1105 96-97 1.0174373070780796 1105 98-99 0.8485644279464886 1105 100-101 1.3208900315041738 1106 1 -0.23544077800581675 1106 2 -0.0655895190124518 1106 3 -0.09200698011787622 1106 4 0.07221076623619638 1106 5 -0.14090441547745058 1106 6 -0.19568103962516403 1106 7 -0.41444334891398427 1106 8 -0.22595357410122574 1106 9 -0.6011691397910539 1106 10-11 -0.7652772504394747 1106 12-13 -0.32801267573930204 1106 14-15 -0.37502464026285054 1106 16-17 -0.32568375932414284 1106 18-19 -0.5400691746055557 1106 20-21 -0.278855943764448 1106 22-23 -0.509601253477463 1106 24-25 -0.2763490762651415 1106 26-27 -0.4931464171199451 1106 28-29 -0.19656157633251325 1106 30-31 -0.30567764026507405 1106 32-33 -0.4627448811255235 1106 34-35 -0.29300051898129453 1106 36-37 -0.5329107045849213 1106 38-39 -0.4217053126194301 1106 40-41 -0.584084385456336 1106 42-43 -0.5676316461525062 1106 44-45 -0.273610226027607 1106 46-47 -0.2046632696030457 1106 48-49 -0.09366040213496518 1106 50-51 -0.2827181088213351 1106 52-53 -0.027983327723404727 1106 54-55 -0.3323079396594508 1106 56-57 -0.2880061823552893 1106 58-59 -0.35124347772061526 1106 60-61 -0.5311904203240445 1106 62-63 -0.5167044621661319 1106 64-65 -0.20254018565226062 1106 66-67 -0.20080590834960788 1106 68-69 -0.48250706710579294 1106 70-71 -0.23607561979839176 1106 72-73 -0.19483108717974318 1106 74-75 -0.10622614843856582 1106 76-77 -0.10030430801792889 1106 78-79 -0.0332108573854093 1106 80-81 -0.24261155055742378 1106 82-83 -0.32057388014411714 1106 84-85 -0.42443409235872664 1106 86-87 -0.3285428550559928 1106 88-89 -0.15514209584068084 1106 90-91 0.19625762350964848 1106 92-93 0.3897308246803952 1106 94-95 0.2547461150860073 1106 96-97 0.5260636117220905 1106 98-99 0.7947408809509859 1106 100-101 0.974199272647386 1107 1 0.07931784697387911 1107 2 -0.018581090153197977 1107 3 0.07449841276234537 1107 4 0.001075202947177445 1107 5 0.1580885449217675 1107 6 0.09014161219970873 1107 7 0.12951527744806413 1107 8 0.01954256742343574 1107 9 0.11785851751136533 1107 10-11 0.03700051140291549 1107 12-13 0.10771338460704527 1107 14-15 0.10851420195263728 1107 16-17 0.15255119123968086 1107 18-19 0.16756276175119922 1107 20-21 0.06919045470390728 1107 22-23 0.2268906269633746 1107 24-25 0.3276146302211771 1107 26-27 0.186954355957468 1107 28-29 0.1509258356724743 1107 30-31 0.15262819012789208 1107 32-33 0.3059885091144139 1107 34-35 0.44624482337415117 1107 36-37 0.47156118712204176 1107 38-39 0.32252425370383975 1107 40-41 0.4240377656134342 1107 42-43 0.48580987885240745 1107 44-45 0.4689734837072237 1107 46-47 0.47475669770445705 1107 48-49 0.3600448055108032 1107 50-51 0.3932727746019111 1107 52-53 0.5147476019393409 1107 54-55 0.3961862009183008 1107 56-57 0.38650559005513685 1107 58-59 0.3469349348615012 1107 60-61 0.4047222568323292 1107 62-63 0.6288134513196937 1107 64-65 0.7524134538939151 1107 66-67 0.540579479554161 1107 68-69 0.5027631644027224 1107 70-71 0.6358758736544701 1107 72-73 0.9044801585565772 1107 74-75 1.0097855738618016 1107 76-77 0.8128696292166175 1107 78-79 0.8573884936027945 1107 80-81 1.091050666104696 1107 82-83 1.230799308549667 1107 84-85 1.1059196501722788 1107 86-87 1.0724405118568434 1107 88-89 1.2407628747326136 1107 90-91 1.149769302611677 1107 92-93 1.1929805490750134 1107 94-95 1.5823173664208348 1107 96-97 1.773434561014156 1107 98-99 1.6998678322198089 1107 100-101 1.9307659380468998 1108 1 0.1220341025973326 1108 2 0.18822090571438466 1108 3 0.15185843093324536 1108 4 0.35918836072622184 1108 5 0.1782908426606653 1108 6 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3998.0 3 816.0 4 523.0 5 521.0 6 678.0 7 779.0 8 1027.0 9 1349.0 10 1845.0 11 2600.0 12 3729.0 13 5505.0 14 6147.0 15 8499.0 16 11024.0 17 15123.0 18 12805.0 19 7898.0 20 7347.0 21 7566.0 22 8180.0 23 9109.0 24 10078.0 25 11640.0 26 13473.0 27 15851.0 28 18763.0 29 22712.0 30 27739.0 31 34532.0 32 43207.0 33 56551.0 34 76171.0 35 106301.0 36 149416.0 37 207064.0 38 181639.0 39 25005.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.840259878906195 19.073216988300604 14.618995884891802 20.4675272479014 2 13.851078638543973 21.965981537755038 41.286132507686624 22.896807316014364 3 20.568710348031505 27.578487803740583 30.755757326813832 21.097044521414084 4 11.260516260964716 16.758480276965575 40.52379500242165 31.45720845964805 5 11.854104706519673 39.894382240144076 34.46649398545603 13.785019067880219 6 27.593822945242962 37.48441293040497 19.539143056554824 15.382621067797247 7 25.602780379449307 35.96990314604887 21.117319237656414 17.30999723684541 8 24.441408559103632 33.19713709832949 21.387353753499465 20.974100589067405 9 26.400879757241537 18.017801450056645 22.13377010242606 33.44754869027576 10-11 23.09824244751205 26.886731519324826 28.320080521596225 21.694945511566907 12-13 27.461283756446726 24.852450810208545 27.78051390806423 19.9057515252805 14-15 23.86935146640547 25.583857834418218 29.326584558365155 21.22020614081116 16-17 19.563258449897173 29.437286523896987 30.316241788949366 20.683213237256474 18-19 19.01892191291166 29.37287246209114 32.7299759159345 18.878229709062694 20-21 21.059483788564272 27.452300312496636 31.112296078902713 20.37591982003638 22-23 19.91377906974137 27.496655609136567 30.449585690177784 22.139979630944282 24-25 20.29211220803914 27.27648191787764 31.924716307206612 20.50668956687661 26-27 21.50407818490612 26.164604664742118 32.43671716317807 19.894599987173684 28-29 19.623918513435328 26.874195472669616 33.60634561821338 19.895540395681678 30-31 19.82570395687003 28.475829094790857 32.466158938615145 19.23230800972398 32-33 19.22315126039113 28.291589408785466 31.632628346757592 20.852630984065815 34-35 20.11395906606136 27.022194790788724 31.769532299541087 21.094313843608834 36-37 21.05807444586257 26.94207085460894 32.32886160668239 19.670993092846103 38-39 21.79231732031376 26.16261105453091 32.57257251870398 19.472499106451355 40-41 20.178491588670212 27.090970976842556 33.35279601095829 19.377741423528942 42-43 19.28135645516304 28.633983492778764 31.847527953029225 20.237132099028976 44-45 19.938460710393052 28.842729437938164 30.544838010827686 20.673971840841094 46-47 19.83304662986994 28.211845076961378 29.444617637757837 22.510490655410845 48-49 20.56421840978884 27.863100487414517 30.190587777226536 21.38209332557011 50-51 21.7556144179464 28.471878006822752 30.018368953552788 19.75413862167806 52-53 20.162720167969205 28.83740545002647 30.651239580435895 20.34863480156843 54-55 19.26017633219173 28.26352489441824 31.314013497419612 21.162285275970422 56-57 20.606293806335245 29.717652767481834 28.842972352762054 20.833081073420864 58-59 20.33052818865495 28.491589049891758 29.640640161277883 21.53724260017541 60-61 20.370358745520615 27.521011417728147 30.649049240073605 21.45958059667763 62-63 22.215196940325313 27.42945084866153 29.946848510856977 20.408503700156185 64-65 21.34001601619914 27.929031916715765 30.78293745701139 19.948014610073706 66-67 20.03922525195095 28.216533434729502 30.88531232232503 20.858928990994514 68-69 19.80283527979499 29.567623194319577 30.254628124558597 20.37491340132684 70-71 19.97193001336239 29.288654523931235 29.041181271130963 21.698234191575416 72-73 20.0017309207467 28.344455022293452 28.940725829776948 22.713088227182897 74-75 20.33567381846457 28.736936227534976 29.566703826240282 21.360686127760175 76-77 21.162931078556326 27.463546286820335 30.086904109490746 21.28661852513259 78-79 20.127816301847734 27.49014650307926 31.038795579570376 21.343241615502624 80-81 19.827733490939373 29.31131512403628 29.999242163680982 20.86170922134336 82-83 19.56588026596403 29.167526129154343 29.463480168388877 21.803113436492747 84-85 20.11692974663876 27.676422620587726 29.40297041734808 22.803677215425434 86-87 20.761551211356945 27.944262871452302 29.86412819252557 21.430057724665186 88-89 21.619099508745496 26.597473996695076 30.210201322418563 21.57322517214087 90-91 19.985389112422215 27.20306891423795 31.176171965393095 21.635370007946744 92-93 19.58830474811872 28.468443259391595 30.670820928619253 21.27243106387043 94-95 19.41605210976197 28.885923351009975 29.76441910583652 21.933605433391538 96-97 19.106003699758954 28.08009417568249 29.168226918549244 23.645675206009305 98-99 20.012063916332927 27.560981078935686 29.35509621982339 23.071858784907995 100-101 21.05202742906543 27.28221688834444 30.178518502243534 21.4872371803466 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1988.0 1 1281.0 2 604.5 3 676.0 4 605.5 5 583.0 6 1068.0 7 2068.0 8 3186.0 9 3551.5 10 2930.5 11 2385.0 12 2319.0 13 2376.0 14 2433.5 15 2445.5 16 2470.5 17 2546.5 18 2709.0 19 2954.5 20 3215.5 21 3578.5 22 4110.5 23 4777.5 24 5611.0 25 6851.0 26 8474.5 27 10460.5 28 12420.5 29 14574.5 30 17320.5 31 19876.5 32 22596.0 33 25886.0 34 29498.0 35 32111.5 36 33686.0 37 35732.5 38 36317.5 39 35290.5 40 34698.0 41 34366.0 42 34588.5 43 35476.0 44 37062.5 45 37669.0 46 38857.5 47 47937.5 48 84675.5 49 82069.5 50 45034.5 51 34174.0 52 25759.0 53 22029.0 54 19616.0 55 17083.0 56 15032.5 57 13306.0 58 11532.0 59 9883.0 60 8530.0 61 7234.0 62 5695.5 63 3734.0 64 2271.5 65 1659.5 66 1170.5 67 792.5 68 571.5 69 420.5 70 330.0 71 280.0 72 228.0 73 207.5 74 188.5 75 154.5 76 106.5 77 59.0 78 43.5 79 35.0 80 26.5 81 19.0 82 10.0 83 6.0 84 4.0 85 5.5 86 3.5 87 0.0 88 0.0 89 0.5 90 2.0 91 1.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2276193681436714 2 0.20515280840947497 3 0.1970970698593647 4 0.2037206771116776 5 0.2015724801649815 6 0.2246655973419643 7 0.2280669091742331 8 0.2150882192879443 9 0.21150789104345086 10-11 0.22233838398304354 12-13 0.22403903989917795 14-15 0.21007575974565348 16-17 0.2222936298799874 18-19 0.21222395669234956 20-21 0.20801707100506975 22-23 0.20492903789419412 24-25 0.1999165783519033 26-27 0.20770379228367658 28-29 0.21759444905908973 30-31 0.2189370721507748 32-33 0.21866854753243778 34-35 0.22533690888780683 36-37 0.20452625096668864 38-39 0.20403395583307077 40-41 0.2024228081230487 42-43 0.20676395611949702 44-45 0.22206985936470658 46-47 0.2267690401856042 48-49 0.22905149944146877 50-51 0.2294542863689743 52-53 0.24399936986222898 54-55 0.22900674533841262 56-57 0.20537657892475583 58-59 0.1899364133703778 60-61 0.18868329848480508 62-63 0.1965152665196345 64-65 0.1875196918053447 66-67 0.18957838054592843 68-69 0.1932034628934781 70-71 0.19208461031707386 72-73 0.1971418239624209 74-75 0.1973655944777017 76-77 0.20667444791338468 78-79 0.20197526709248703 80-81 0.19687329934408387 82-83 0.19494887291266863 84-85 0.19579920087073582 86-87 0.20300461146277893 88-89 0.1981264142296566 90-91 0.20533182482169965 92-93 0.19839493884799358 94-95 0.195575430355455 96-97 0.20394444762695846 98-99 0.20774854638673274 100-101 0.18989165926732163 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1117216.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.49754973384192 #Duplication Level Percentage of deduplicated Percentage of total 1 77.93187757355008 43.25028251489776 2 13.149802696309468 14.595636582572869 3 4.269379377792941 7.108202830551084 4 1.8840211401792823 4.182342277068372 5 0.9041136832096102 2.5088047049486173 6 0.5027881589891994 1.6742106515453947 7 0.32139012701655606 1.2485455190646966 8 0.20227891964294198 0.8980787522393587 9 0.14040430990516165 0.7012885654626717 >10 0.6138727304777658 5.976651551901342 >50 0.04125366558209005 1.5433010111331102 >100 0.02818706824639659 3.1110164311954387 >500 0.004832067772072399 1.84749820050608 >1k 0.004509929920600906 5.299981411176987 >5k 9.664135544144798E-4 3.696873576462645 >10k+ 3.2213785147149326E-4 2.357285419273555 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 14112 1.2631398046572908 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 12259 1.0972810987311317 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 9147 0.8187315613095408 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8985 0.8042312319193423 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6492 0.5810872740812878 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 5972 0.5345430069028729 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 5618 0.5028571019391058 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 5143 0.460340704035746 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 4507 0.40341348494830004 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4430 0.39652135307765013 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 3835 0.34326397044081003 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3438 0.3077292126142125 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 3047 0.2727315040242889 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2563 0.22940953226591815 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 2460 0.2201901870363475 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2409 0.21562526852461836 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2343 0.20971772692120416 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2318 0.20748002176839572 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 2263 0.2025570704322172 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 2164 0.19369575802709593 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 2144 0.1919055939048492 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2002 0.1791954286368974 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1823 0.1631734597427892 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1651 0.14777804829146737 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1621 0.14509280210809727 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1537 0.137574112794661 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1531 0.13703706355798698 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1522 0.13623148970297597 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1379 0.12343181622891186 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1307 0.11698722538882365 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1279 0.11448099561767824 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1272 0.11385443817489187 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1194 0.10687279809812963 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1182 0.1057986996247816 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 8.950820611233638E-5 0.0 0.0 0.0 3 0.0 8.950820611233638E-5 0.0 0.0 0.0 4 0.0 8.950820611233638E-5 0.0 0.0 0.0 5 0.0 8.950820611233638E-5 0.0 0.0 0.0 6 0.0 8.950820611233638E-5 0.0 0.0 0.0 7 0.0 8.950820611233638E-5 0.0 0.0 0.0 8 0.0 8.950820611233638E-5 0.0 0.0 0.0 9 0.0 8.950820611233638E-5 0.0 0.0 0.0 10-11 0.0 1.3426230916850458E-4 0.0 4.475410305616819E-5 0.0 12-13 0.0 1.7901641222467277E-4 0.0 8.950820611233638E-5 0.0 14-15 0.0 1.7901641222467277E-4 0.0 2.2377051528084093E-4 0.0 16-17 0.0 1.7901641222467277E-4 0.0 2.685246183370091E-4 0.0 18-19 0.0 1.7901641222467277E-4 0.0 4.027869275055137E-4 0.0 20-21 0.0 2.6852461833700916E-4 0.0 4.922951336178501E-4 0.0 22-23 0.0 4.027869275055137E-4 0.0 5.818033397301865E-4 0.0 24-25 0.0 4.475410305616819E-4 0.0 0.0011188525764042048 0.0 26-27 0.0 4.475410305616819E-4 0.0 0.0019691805344714004 0.0 28-29 0.0 4.475410305616819E-4 0.0 0.004296393893392146 0.0 30-31 0.0 4.475410305616819E-4 0.0 0.009801148569300835 0.0 32-33 0.0 4.475410305616819E-4 0.0 0.018483444562197464 0.0 34-35 0.0 4.475410305616819E-4 0.0 0.02716574055509409 0.0 36-37 0.0 4.475410305616819E-4 0.0 0.03822000400996764 0.0 38-39 0.0 4.475410305616819E-4 0.0 0.04958754618623436 0.0 40-41 0.0 6.713115458425228E-4 0.0 0.0656990232864549 0.0 42-43 0.0 7.160656488986911E-4 0.0 0.08646492710451695 0.0 44-45 0.0 7.160656488986911E-4 0.0 0.1049483716667144 0.0 46-47 0.0 7.160656488986911E-4 0.0 0.12956312834760692 0.0 48-49 0.0 7.160656488986911E-4 0.0 0.1542226391315556 0.0 50-51 0.0 7.160656488986911E-4 0.0 0.1874301835992324 0.0 52-53 0.0 7.608197519548593E-4 0.0 0.2630198636611005 0.0 54-55 0.0 8.055738550110274E-4 0.0 0.36922135021338753 0.0 56-57 0.0 8.055738550110274E-4 0.0 0.45425414602010716 0.0 58-59 0.0 9.845902672357002E-4 0.0 0.5968854724601151 0.0 60-61 0.0 9.845902672357002E-4 0.0 0.7820779509065392 0.0 62-63 0.0 9.845902672357002E-4 0.0 0.8979015696159024 0.0 64-65 0.0 0.001163606679460373 0.0 0.9856643657090483 0.0 66-67 0.0 0.001163606679460373 0.0 1.2053175035087216 0.0 68-69 0.0 0.001163606679460373 0.0 1.419868673559992 0.0 70-71 0.0 0.001163606679460373 0.0 1.5453591785294876 0.0 72-73 0.0 0.001163606679460373 0.0 1.793252155357603 0.0 74-75 0.0 0.001432131297797382 0.0 2.125014321312978 0.0 76-77 0.0 0.0017006559161343911 0.0 2.4434845186606706 0.0 78-79 0.0 0.0017006559161343911 0.0 2.762849798069487 0.0 80-81 0.0 0.0017454100191905595 0.0 3.1776308251940537 0.0 82-83 0.0 0.0017901641222467276 0.0 3.6188615272248157 0.0 84-85 0.0 0.0017901641222467276 0.0 4.147720763039556 0.0 86-87 0.0 0.0018796723283590639 0.0 4.851076246670295 0.0 88-89 0.0 0.0018796723283590639 0.0 5.540513204250566 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAATACG 50 0.0016133712 38.011616 4 CGTACAC 55 0.002576864 34.55291 3 TATAACG 80 4.489058E-4 29.692577 2 CATTCCG 130 3.0322008E-7 29.235767 9 ATAACGC 70 0.008382632 27.148716 3 GTAGCCC 520 0.0 26.496103 3 GTGTAGC 665 0.0 25.718681 1 TAGCCCT 795 0.0 23.906673 4 GAGTACT 6240 0.0 22.416674 12-13 AGTACTT 6335 0.0 22.080511 12-13 TACCTGG 1065 0.0 21.858196 2 GTACATG 10825 0.0 20.232096 1 GTACTTT 6945 0.0 20.106918 14-15 TGTAGCC 810 0.0 19.94168 2 TACATGG 10845 0.0 19.931946 2 GTATAGG 340 4.1836756E-11 19.562166 1 ACATGGG 10895 0.0 19.100037 3 ACCTGGG 1145 0.0 19.08709 3 CGTCACT 150 7.7119854E-4 19.003248 1 GTCCTAC 280 3.481182E-8 18.663904 1 >>END_MODULE