##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937380_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1117216 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.154109858791855 33.0 31.0 34.0 31.0 34.0 2 32.33644434021711 34.0 31.0 34.0 31.0 34.0 3 32.38857749978518 34.0 31.0 34.0 30.0 34.0 4 36.0160013820067 37.0 35.0 37.0 35.0 37.0 5 35.95574803798012 37.0 35.0 37.0 35.0 37.0 6 36.034155436370405 37.0 35.0 37.0 35.0 37.0 7 35.98275266376422 37.0 35.0 37.0 35.0 37.0 8 35.982417007991295 37.0 35.0 37.0 35.0 37.0 9 37.653557593160144 39.0 37.0 39.0 35.0 39.0 10-11 37.57653041130811 39.0 37.0 39.0 35.0 39.0 12-13 37.487439313436255 39.0 37.0 39.0 35.0 39.0 14-15 38.558136027410995 40.0 38.0 41.0 34.0 41.0 16-17 38.55596679603586 40.0 38.0 41.0 34.0 41.0 18-19 38.6593617527855 40.0 38.0 41.0 34.0 41.0 20-21 38.62299501618308 40.0 38.0 41.0 34.0 41.0 22-23 38.49293959270186 40.0 38.0 41.0 34.0 41.0 24-25 38.433885211096154 40.0 38.0 41.0 34.0 41.0 26-27 38.29903886088277 40.0 38.0 41.0 34.0 41.0 28-29 38.15914782817289 40.0 37.5 41.0 34.0 41.0 30-31 38.21216711898147 40.0 37.5 41.0 34.0 41.0 32-33 38.121515893077074 40.0 37.0 41.0 34.0 41.0 34-35 37.98671966745911 40.0 37.0 41.0 33.5 41.0 36-37 37.778877137455964 40.0 37.0 41.0 33.0 41.0 38-39 37.59965798914445 40.0 36.5 41.0 33.0 41.0 40-41 37.38005273823504 40.0 36.0 41.0 33.0 41.0 42-43 37.18243741586228 40.0 36.0 41.0 32.5 41.0 44-45 36.869910115859426 40.0 36.0 41.0 31.5 41.0 46-47 36.40033708790422 40.0 36.0 41.0 30.5 41.0 48-49 35.987755277403835 39.0 36.0 41.0 29.5 41.0 50-51 35.720705306762525 39.0 35.0 41.0 28.0 41.0 52-53 35.408572290407584 39.0 35.0 40.5 27.0 41.0 54-55 35.20537031335033 39.0 35.0 40.0 27.0 41.0 56-57 35.025785971557866 39.0 35.0 40.0 26.0 41.0 58-59 34.819475822043366 38.0 35.0 40.0 26.0 41.0 60-61 34.487048610116574 38.0 34.0 40.0 25.0 41.0 62-63 34.07866518202388 38.0 34.0 40.0 23.5 41.0 64-65 33.63542099289663 37.0 33.0 40.0 22.0 41.0 66-67 33.23092222094922 36.5 33.0 39.0 21.5 41.0 68-69 32.86112891329877 36.0 32.5 39.0 20.5 40.0 70-71 32.45643814624925 36.0 32.0 39.0 19.0 40.0 72-73 31.954755839515368 35.0 31.5 38.0 17.5 40.0 74-75 31.480036537249735 35.0 31.0 37.0 13.5 39.0 76-77 30.122022509523674 33.5 29.5 36.0 10.5 38.5 78-79 30.678692840059576 34.0 31.0 36.0 7.5 39.0 80-81 30.524701579640823 34.0 31.0 36.0 7.0 38.0 82-83 30.202104158909286 34.0 31.0 35.5 2.0 37.0 84-85 29.84346491636353 34.0 30.0 35.0 2.0 37.0 86-87 29.404681368687882 34.0 30.0 35.0 2.0 36.0 88-89 28.862970544639534 34.0 29.0 35.0 2.0 36.0 90-91 28.31655069386761 34.0 29.0 35.0 2.0 35.5 92-93 28.056091659983387 34.0 29.0 35.0 2.0 35.0 94-95 27.69155516927792 33.5 27.5 35.0 2.0 35.0 96-97 27.18973188711941 33.0 27.0 35.0 2.0 35.0 98-99 26.474307564517517 33.0 25.5 35.0 2.0 35.0 100-101 24.098999208747458 30.5 13.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.009014016438783301 1101 2 -0.002071889013642192 1101 3 0.004601778484328634 1101 4 0.06218134841259371 1101 5 -0.04452228993695684 1101 6 -0.09216449481336042 1101 7 -0.10131980997535095 1101 8 -0.042680082573802736 1101 9 -0.08409325242762122 1101 10-11 -0.08156967386657499 1101 12-13 -0.05189267710613166 1101 14-15 -0.14838485927479184 1101 16-17 -0.01546192013855574 1101 18-19 -0.036458508759025676 1101 20-21 0.006553002298176125 1101 22-23 0.08230128734420106 1101 24-25 -0.01311390693462755 1101 26-27 0.04799877758100024 1101 28-29 -0.06996154167147495 1101 30-31 -0.09638596351751971 1101 32-33 0.1324734682022708 1101 34-35 0.16633687574532274 1101 36-37 0.30472808356333303 1101 38-39 0.3554722080511965 1101 40-41 0.26363875095777445 1101 42-43 0.1856984008644602 1101 44-45 0.14674153736909545 1101 46-47 0.3800445366109315 1101 48-49 0.30836466980185406 1101 50-51 0.46419959072146355 1101 52-53 0.4970113589820784 1101 54-55 0.47121757767896355 1101 56-57 0.5893438407827176 1101 58-59 0.6190430279739516 1101 60-61 0.6734918871780735 1101 62-63 0.5951182181635417 1101 64-65 0.6429325235494829 1101 66-67 0.6068331306290347 1101 68-69 0.499281195596609 1101 70-71 0.5722780504235416 1101 72-73 0.6169418641297675 1101 74-75 0.5576703867456061 1101 76-77 0.6994972790444685 1101 78-79 0.42208386712897905 1101 80-81 0.5547929601796255 1101 82-83 0.6684304886178936 1101 84-85 0.6282952783949014 1101 86-87 0.6284231211820561 1101 88-89 0.6697065402073257 1101 90-91 0.4329422512842207 1101 92-93 0.5400102773117474 1101 94-95 0.6610910870181925 1101 96-97 0.6079492123431187 1101 98-99 0.6661065539366398 1101 100-101 1.2496985746307203 1102 1 -0.11697257731253785 1102 2 -0.051599073401689566 1102 3 -0.045273743520169774 1102 4 -0.08798980883122454 1102 5 -0.07407376761122464 1102 6 -0.08958709760808858 1102 7 -0.16012060502835368 1102 8 -0.1587000994385619 1102 9 -0.12463443055162315 1102 10-11 -0.17747495012741865 1102 12-13 -0.23189814811480147 1102 14-15 -0.29576730787742633 1102 16-17 -0.25735253008073045 1102 18-19 -0.262369256028542 1102 20-21 -0.037182692770890924 1102 22-23 -0.14273911781159399 1102 24-25 -0.18494649406921582 1102 26-27 -0.129191884561628 1102 28-29 -0.23069124372736383 1102 30-31 -0.3461349998169041 1102 32-33 -0.204301748630364 1102 34-35 -0.297306765838357 1102 36-37 -0.4184383756400152 1102 38-39 -0.3712536693179018 1102 40-41 -0.3205061153287616 1102 42-43 -0.2562313594150112 1102 44-45 -0.4088408991872754 1102 46-47 -0.3188499682878785 1102 48-49 -0.28746080000220076 1102 50-51 -0.35835326223388364 1102 52-53 -0.26408826356851733 1102 54-55 -0.33336326274827854 1102 56-57 -0.41147053642576026 1102 58-59 -0.27900045901608905 1102 60-61 -0.35313159700117325 1102 62-63 -0.24925291699546648 1102 64-65 -0.2999777519082798 1102 66-67 -0.2896031848222975 1102 68-69 -0.2656453724245935 1102 70-71 -0.22943151430501985 1102 72-73 -0.10776626508642195 1102 74-75 -0.2251268044686583 1102 76-77 -0.041847484848883454 1102 78-79 0.019193016261649376 1102 80-81 -0.15895056495689985 1102 82-83 -0.1482161846072927 1102 84-85 -0.2883338273712397 1102 86-87 -0.27794658850313425 1102 88-89 -0.07015000894140755 1102 90-91 -0.05865899751115222 1102 92-93 -0.1638676137900852 1102 94-95 -0.06847193498630233 1102 96-97 -0.06763979946221355 1102 98-99 0.21096982891254612 1102 100-101 0.5396457216753738 1103 1 -0.04278323162637321 1103 2 -0.0821162274260061 1103 3 -0.18883917490406787 1103 4 -0.10047985660192182 1103 5 -0.11922069870560392 1103 6 -0.07021191665846516 1103 7 -0.11085706999650569 1103 8 -0.1836756712393779 1103 9 -0.16689973888977505 1103 10-11 -0.14947299587148422 1103 12-13 -0.1654828506336301 1103 14-15 -0.14017325026306793 1103 16-17 -0.17908050851153945 1103 18-19 -0.18188512531053647 1103 20-21 -0.24750722592086305 1103 22-23 -0.24046322392045738 1103 24-25 -0.205789628840499 1103 26-27 -0.3905849252391036 1103 28-29 -0.39899701240137375 1103 30-31 -0.4105711607896936 1103 32-33 -0.32146662992740715 1103 34-35 -0.2954271516229525 1103 36-37 -0.32564288490463156 1103 38-39 -0.2302961743844918 1103 40-41 -0.43161596984526795 1103 42-43 -0.23806175533279372 1103 44-45 -0.008553189285713358 1103 46-47 0.09854879039770026 1103 48-49 -0.10469715640057586 1103 50-51 0.045062614286088376 1103 52-53 0.056509232088693295 1103 54-55 -0.04938318530183494 1103 56-57 -0.0831216112665274 1103 58-59 -0.19474126677521042 1103 60-61 -0.16764239969370465 1103 62-63 -0.0075381478871818786 1103 64-65 -0.23425232486716396 1103 66-67 -0.06104571522183733 1103 68-69 -0.1281802052271459 1103 70-71 0.06541684991038466 1103 72-73 0.006573540211782358 1103 74-75 0.21197284448907538 1103 76-77 0.1653941190885817 1103 78-79 0.0308212914498327 1103 80-81 -0.19960768351509373 1103 82-83 -0.06886751277752623 1103 84-85 -0.1818834256630737 1103 86-87 0.007028711873243054 1103 88-89 -0.012693979699943014 1103 90-91 0.1610183693099252 1103 92-93 0.0550180984276345 1103 94-95 0.2646246559227876 1103 96-97 0.16095612193327113 1103 98-99 0.20473611437867234 1103 100-101 0.6385233816348403 1104 1 -0.0036017619876531626 1104 2 0.053785834297379154 1104 3 0.0706655649554051 1104 4 -0.08455885597062718 1104 5 -0.08007836434406101 1104 6 -0.04238416833550929 1104 7 -0.06721417875921532 1104 8 -0.09413351548867155 1104 9 0.19986388916854736 1104 10-11 0.0067778060809189355 1104 12-13 0.12004235761737192 1104 14-15 0.04517783808257292 1104 16-17 -0.14971589860684276 1104 18-19 -0.016063181839832907 1104 20-21 -0.030616953539336578 1104 22-23 -0.00876570240246366 1104 24-25 0.016342845997442623 1104 26-27 -0.06116372957298921 1104 28-29 0.057931728032940555 1104 30-31 0.0387566747856809 1104 32-33 0.10971141890315295 1104 34-35 0.20708937501213143 1104 36-37 0.21361525138365067 1104 38-39 0.38511119177286446 1104 40-41 0.38447228597883054 1104 42-43 0.3917239758168307 1104 44-45 0.39449653946190466 1104 46-47 0.19078903480713194 1104 48-49 0.26255213403194233 1104 50-51 0.13136103296427137 1104 52-53 0.399817997877534 1104 54-55 0.6002184689014172 1104 56-57 0.46945021800615194 1104 58-59 0.23421858171352739 1104 60-61 0.42345843074404144 1104 62-63 0.3332127105226661 1104 64-65 0.3699989210460828 1104 66-67 0.5005564188697349 1104 68-69 0.48979693720473705 1104 70-71 0.510469637625242 1104 72-73 0.42692298615891033 1104 74-75 0.5794213398980546 1104 76-77 0.4334633898123137 1104 78-79 0.3895599422194529 1104 80-81 0.5488698900464009 1104 82-83 0.4634803600597657 1104 84-85 0.5103837001908431 1104 86-87 0.4759161665148106 1104 88-89 0.53635374994073 1104 90-91 0.5488771948923343 1104 92-93 0.815484860387631 1104 94-95 0.8782427058824993 1104 96-97 0.6759930502374409 1104 98-99 1.052907919528618 1104 100-101 1.658439388348146 1105 1 -0.058980870224445425 1105 2 -0.02540256659523976 1105 3 0.009168049626445907 1105 4 -0.028004331206439304 1105 5 -0.1830698167428153 1105 6 -0.18804220048713916 1105 7 -0.057953006061055135 1105 8 -0.1157209588502397 1105 9 0.004210087129493445 1105 10-11 -0.051235414061075346 1105 12-13 -0.09938086306551241 1105 14-15 -0.18762328698867492 1105 16-17 -0.13154756509089793 1105 18-19 -0.02986028344991354 1105 20-21 -0.033243437372675544 1105 22-23 -0.13478665047471594 1105 24-25 -0.19634374704215674 1105 26-27 -0.23649426779586946 1105 28-29 -0.26872935396050934 1105 30-31 -0.43214951241274235 1105 32-33 -0.31434419112762413 1105 34-35 -0.30569319304067477 1105 36-37 -0.33834405354644304 1105 38-39 -0.3470356527711971 1105 40-41 -0.3585546161502364 1105 42-43 -0.43447456763751546 1105 44-45 -0.4176545629331727 1105 46-47 -0.4837981131400184 1105 48-49 -0.33354978255578516 1105 50-51 -0.37518662653524615 1105 52-53 -0.3663340975538034 1105 54-55 -0.296363600136786 1105 56-57 -0.45726308160648443 1105 58-59 -0.3560790656504125 1105 60-61 -0.45847565994125006 1105 62-63 -0.36987603446534223 1105 64-65 -0.3350942731881972 1105 66-67 -0.3301142550773477 1105 68-69 -0.30944781433509405 1105 70-71 -0.2599309471011395 1105 72-73 -0.2917658592422647 1105 74-75 -0.3217427732137672 1105 76-77 -0.055641296458961875 1105 78-79 -0.22862801624500406 1105 80-81 -0.5193493435126797 1105 82-83 -0.5319192191480546 1105 84-85 -0.4483935060209028 1105 86-87 -0.2128165472341621 1105 88-89 -0.16274482000383372 1105 90-91 -0.16776628705895646 1105 92-93 -0.03531525500429211 1105 94-95 0.18200847692106592 1105 96-97 0.0938085507568438 1105 98-99 0.20629284174308893 1105 100-101 0.9172830974772808 1106 1 -0.13461010431934994 1106 2 -0.004905850034560899 1106 3 -0.040859168150383596 1106 4 -0.03595884448021991 1106 5 -0.16783415484739095 1106 6 -0.024535732725261994 1106 7 -0.1116459706590689 1106 8 -0.09325921319957331 1106 9 -0.07799703857887863 1106 10-11 -0.27177807045315916 1106 12-13 -0.24936103971361234 1106 14-15 -0.17223580339014433 1106 16-17 -0.04638581597901492 1106 18-19 -0.09259961895856605 1106 20-21 -0.0650237317022544 1106 22-23 -0.18553128479680225 1106 24-25 -0.003232833814941216 1106 26-27 -0.05222441219167706 1106 28-29 -0.04412999019571373 1106 30-31 -0.07204180553404171 1106 32-33 -0.35565936039701995 1106 34-35 -0.3025308964266955 1106 36-37 -0.3325268437304203 1106 38-39 -0.17976432142858556 1106 40-41 -0.2633639815827138 1106 42-43 -0.5270999660711553 1106 44-45 -0.3564554704021745 1106 46-47 -0.5607953158447998 1106 48-49 -0.5314167269591508 1106 50-51 -0.36605215183833906 1106 52-53 -0.25829997621601564 1106 54-55 -0.49473871459304775 1106 56-57 -0.6111748897164375 1106 58-59 -0.5632393862223424 1106 60-61 -0.6372893739248937 1106 62-63 -0.6049644686408087 1106 64-65 -0.6511091816074597 1106 66-67 -0.353147102056802 1106 68-69 -0.3088712065196617 1106 70-71 -0.36250499361364774 1106 72-73 -0.4104652585632387 1106 74-75 -0.3804049486850438 1106 76-77 -0.14166363076877886 1106 78-79 -0.12731180860954794 1106 80-81 -0.326211684493213 1106 82-83 -0.4718363701233308 1106 84-85 -0.5282108165269648 1106 86-87 -0.36408209519251145 1106 88-89 -0.15622513982058095 1106 90-91 -0.07509290352743747 1106 92-93 0.08306555473243549 1106 94-95 0.12568498499042846 1106 96-97 0.17570652657235541 1106 98-99 0.26943179085371227 1106 100-101 0.9630482945937722 1107 1 -0.020628703794749015 1107 2 0.0343666208756801 1107 3 0.026849903154520405 1107 4 -0.006335153821773076 1107 5 0.08304622723325394 1107 6 -0.07413260585693138 1107 7 -0.01754617213610743 1107 8 -0.022042437039523577 1107 9 0.08303253327378002 1107 10-11 0.09729036607811992 1107 12-13 0.04136548717476529 1107 14-15 0.09855382762695086 1107 16-17 0.12283588824715963 1107 18-19 0.09993190718679301 1107 20-21 0.15724034490740735 1107 22-23 0.21065533892495836 1107 24-25 0.2988384399830224 1107 26-27 0.07143255155282446 1107 28-29 0.09149550442523235 1107 30-31 0.21743369755377984 1107 32-33 0.07656158699431614 1107 34-35 0.15327202239845406 1107 36-37 -0.10655462198817389 1107 38-39 -0.029907567109617617 1107 40-41 -0.04049599224060785 1107 42-43 -0.12866098112836966 1107 44-45 -0.14934882580697462 1107 46-47 -0.08944186015074251 1107 48-49 -0.14299198018779435 1107 50-51 -0.3164008488037453 1107 52-53 -0.3258068793100506 1107 54-55 -0.21577768640354122 1107 56-57 -0.4026020936091257 1107 58-59 -0.3159349623671801 1107 60-61 -0.20295343601990368 1107 62-63 -0.19798430877240492 1107 64-65 -0.30413842169689786 1107 66-67 -0.2724664345215615 1107 68-69 -0.16172730767979004 1107 70-71 0.031956288582073 1107 72-73 0.19176101086305763 1107 74-75 0.1454769021390092 1107 76-77 0.23353810545873088 1107 78-79 0.08958271487824732 1107 80-81 0.26462750365454824 1107 82-83 0.19795382420006646 1107 84-85 0.21023967645244213 1107 86-87 0.1004494793349835 1107 88-89 0.1970041949596073 1107 90-91 0.2997641099815418 1107 92-93 0.46811651148917477 1107 94-95 0.32747909826683497 1107 96-97 0.5151365850110672 1107 98-99 0.5031028199763057 1107 100-101 1.1788334848219613 1108 1 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0.47832262635963474 2316 80-81 0.5967070119347717 2316 82-83 0.5552843427470897 2316 84-85 0.4655077791883713 2316 86-87 0.09185174097427407 2316 88-89 0.18930409421591676 2316 90-91 0.15420079426892386 2316 92-93 0.6015430421707215 2316 94-95 0.713726452329972 2316 96-97 0.815902916242738 2316 98-99 0.764591562717662 2316 100-101 0.4329215280172356 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 16.0 5 21.0 6 52.0 7 106.0 8 306.0 9 582.0 10 968.0 11 1749.0 12 2800.0 13 4297.0 14 5315.0 15 7638.0 16 10790.0 17 16247.0 18 14240.0 19 7809.0 20 6036.0 21 5786.0 22 6074.0 23 6672.0 24 7374.0 25 8686.0 26 10188.0 27 12620.0 28 15520.0 29 19682.0 30 24565.0 31 30740.0 32 39605.0 33 51833.0 34 70845.0 35 102894.0 36 154730.0 37 231800.0 38 215353.0 39 23269.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.218367288440454 19.998548876005934 15.017968856370972 19.76511497918264 2 14.325967404691662 23.320109987683672 40.13422650588606 22.219696101738606 3 21.200197634119096 27.98124982098359 30.555595336980495 20.26295720791682 4 11.655311058917881 17.620227422450093 39.66045957093346 31.064001947698568 5 11.680761477842742 40.96854983369077 33.72255181722813 13.62813687123835 6 27.598781256265575 38.39991550425343 18.441196688912438 15.560106550568555 7 25.64678629736774 36.49535989459514 20.65366052759717 17.20419328043995 8 24.935464583393006 34.11766390742703 20.3774382035345 20.56943330564546 9 26.654559189986536 18.379973075931602 21.58848423223441 33.37698350184745 10-11 23.28697846295407 27.912679257206204 27.292655081007272 21.507687198832453 12-13 27.772471673759043 25.525214785322092 27.356372665736927 19.34594087518193 14-15 24.04539498181193 26.37193702918684 28.725286784292386 20.857381204708847 16-17 19.435498596511326 30.221953498696763 29.89274231661559 20.44980558817632 18-19 19.11555151376278 29.537976541689343 32.667317689685795 18.679154254862084 20-21 21.06830729241257 27.82201472230974 31.367031979491877 19.74264600578581 22-23 20.101986380333834 27.742148283936384 30.633194534947833 21.52267080078195 24-25 20.411356360708606 27.821266704051578 31.878976715584812 19.888400219655004 26-27 21.57806358870437 26.637806108246338 32.59250043187903 19.191629871170264 28-29 19.861794876385183 27.150280169713024 33.55623086700442 19.431694086897366 30-31 19.75896248876668 29.044621790671783 32.38418491745524 18.812230803106303 32-33 19.36304170366339 28.723675636582364 31.795552516254688 20.117730143499557 34-35 20.291510325666657 27.326184014550453 31.90546859336064 20.47683706642225 36-37 21.140012826531677 27.415704490315427 32.34599770590365 19.098284977249243 38-39 21.834887431385845 26.42351577482249 32.863769711535234 18.87782708225643 40-41 20.3275646708846 27.53405903700677 33.194744608130314 18.943631683978325 42-43 19.37090287021761 29.019338586849976 31.95544148965216 19.654317053280256 44-45 20.167568537644293 28.980334573172833 30.52251828095679 20.329578608226086 46-47 20.181952281385158 28.663347105662645 29.415574069830726 21.739126543121472 48-49 20.992807120556815 28.200365909546587 29.981086916048465 20.825740053848136 50-51 21.95685525448973 29.062508950820607 29.7237508234755 19.256884971214163 52-53 20.591810357173546 29.491208503995647 30.14403660527597 19.77294453355484 54-55 19.30280268094979 28.939614183828372 30.817809626786584 20.939773508435252 56-57 20.87304514077851 30.101609715578725 28.460252985993755 20.565092157649012 58-59 20.78787808266262 29.08681042878011 29.023617635264802 21.10169385329247 60-61 20.56477887892762 28.3288996935239 30.130610374359122 20.975711053189354 62-63 22.454834159195716 27.982010640735545 29.642566880531607 19.920588319537135 64-65 21.771886546558587 28.51055659782889 30.138397588290893 19.57915926732163 66-67 20.250381304958037 28.868947455102685 30.366956792598742 20.51371444734053 68-69 20.18481654398075 29.95204150316501 29.758300991034858 20.104840961819377 70-71 20.280277045799558 30.105324306132385 28.5363797152923 21.07801893277576 72-73 20.468915590181307 28.767668919886574 28.390973634462807 22.37244185546931 74-75 20.732696273594364 29.598484089021284 28.660393334860938 21.008426302523414 76-77 21.443078151404922 28.24015230716352 29.47281456763956 20.843954973792 78-79 20.52996018674992 28.04529294245696 30.281476455761464 21.14327041503165 80-81 20.039866972844543 30.14539271966778 29.24775927294242 20.566981034545258 82-83 20.028580098119335 29.83751502625735 28.794157952257898 21.339746923365414 84-85 20.362349390311035 28.425567642222717 28.65820092748984 22.553882039976404 86-87 20.874163993354912 28.700045470168707 28.93643664251139 21.489353893965 88-89 21.802050812018443 27.404816973620143 29.61754933692321 21.175582877438202 90-91 20.233261362293618 27.957615860841667 30.273489950909305 21.535632825955407 92-93 19.46704464030369 29.26228455346046 29.871359769707002 21.399311036528847 94-95 19.45232613926045 29.675998195514563 28.83300991034858 22.03866575487641 96-97 19.25630316787443 28.859325322945605 28.342952481883536 23.541419027296424 98-99 20.19743764284982 28.14068215251606 28.39009732696571 23.27178287766841 100-101 20.683885856696925 28.7399280449956 28.68926054998402 21.88692554832345 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1319.0 1 895.5 2 602.0 3 736.0 4 626.5 5 617.5 6 1188.5 7 2277.0 8 3209.0 9 3519.0 10 3058.5 11 2442.5 12 2373.0 13 2475.0 14 2552.5 15 2630.5 16 2669.0 17 2732.0 18 2935.5 19 3221.0 20 3528.0 21 3878.0 22 4331.5 23 5038.5 24 5939.5 25 7087.5 26 8597.5 27 10520.5 28 12431.5 29 14583.5 30 17376.5 31 19996.0 32 22632.0 33 25917.0 34 29445.5 35 31858.0 36 33684.0 37 35643.0 38 35913.0 39 35099.5 40 34512.5 41 34189.5 42 34489.5 43 35320.5 44 36966.0 45 37181.5 46 37852.0 47 46393.5 48 87387.5 49 84655.0 50 44299.5 51 34434.5 52 25422.0 53 21547.5 54 19176.0 55 16801.5 56 14830.5 57 13064.5 58 11282.0 59 9775.0 60 8460.5 61 6989.5 62 5342.5 63 3605.0 64 2276.5 65 1586.0 66 1130.5 67 782.0 68 543.0 69 404.0 70 334.5 71 258.0 72 180.5 73 199.5 74 195.0 75 139.0 76 94.5 77 59.0 78 42.5 79 28.5 80 18.0 81 16.0 82 13.0 83 4.5 84 3.5 85 4.0 86 1.0 87 1.0 88 0.5 89 0.5 90 1.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07518689313436255 2 0.0 3 0.0 4 0.0 5 0.0017901641222467274 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.3426230916850456E-4 12-13 0.004833443130066164 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.160656488986911E-4 24-25 8.503279580671956E-4 26-27 4.4754103056168185E-4 28-29 0.0032222954200441098 30-31 3.5803282444934553E-4 32-33 0.0 34-35 0.0 36-37 4.475410305616819E-5 38-39 7.608197519548592E-4 40-41 1.3426230916850456E-4 42-43 0.011009509351817375 44-45 1.3426230916850456E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 4.475410305616819E-5 82-83 4.4754103056168185E-4 84-85 6.265574427863546E-4 86-87 0.0 88-89 0.0 90-91 0.008950820611233638 92-93 0.0027300002864262597 94-95 0.0 96-97 0.0 98-99 2.2377051528084093E-4 100-101 0.011546558588491393 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1117216.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.6047066568301 #Duplication Level Percentage of deduplicated Percentage of total 1 76.52814775427548 41.02268911358489 2 13.904125613305526 14.906531496419214 3 4.579800575654251 7.364965992141833 4 1.9830376997876653 4.252006167462117 5 1.020620849827049 2.735504063141181 6 0.5323253617396212 1.7121086917226025 7 0.35031180339076595 1.3144853021431007 8 0.2081982677358656 0.8928325654753002 9 0.1514348969359105 0.7305860905071103 >10 0.6552300147009219 6.137850508448387 >50 0.04464487273731181 1.661850198883761 >100 0.0304216551836992 3.1994834608321128 >500 0.005181709664304254 1.9042964051507398 >1k 0.005181709664304254 5.855982489767645 >5k 8.357596232748796E-4 2.7815827225097434 >10k+ 5.014557739649278E-4 3.527244731810287 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 14711 1.3167552201185806 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 14587 1.3056562025606506 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10068 0.9011686191390027 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8014 0.7173187637842637 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 7253 0.6492030189327758 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5481 0.4905944777017157 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 5253 0.47018660670810297 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 5043 0.4513898834245123 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 4823 0.4316980780797983 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4647 0.4159446338040272 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 4121 0.36886331738893824 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 3811 0.3411157734941139 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 3759 0.3364613467762725 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3073 0.2750587173832097 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 2860 0.25599346948128204 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2837 0.2539347807406983 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 2533 0.22672428608254805 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2270 0.20318362787500355 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2113 0.18913083951536677 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2026 0.1813436255835935 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1958 0.17525706756795464 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1946 0.1741829690946066 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1765 0.15798198378827372 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1724 0.1543121473376679 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1710 0.1530590324520952 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1625 0.1454508349325466 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1410 0.1262065706183943 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1311 0.117345258213273 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1272 0.11385443817489187 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1260 0.11278033970154384 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1251 0.11197476584653282 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1217 0.10893148683871337 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1206 0.10794689657147767 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1191 0.10660427347979262 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1187 0.10624624065534327 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1150 0.10293443702918684 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1144 0.10239738779251283 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.3426230916850458E-4 0.0 16-17 0.0 0.0 0.0 1.7901641222467277E-4 0.0 18-19 0.0 0.0 0.0 2.685246183370091E-4 0.0 20-21 0.0 2.2377051528084095E-4 0.0 3.1327872139317735E-4 0.0 22-23 0.0 3.5803282444934553E-4 0.0 4.027869275055137E-4 0.0 24-25 0.0 3.5803282444934553E-4 0.0 9.398361641795319E-4 0.0 26-27 0.0 3.5803282444934553E-4 0.0 0.0017006559161343911 0.0 28-29 0.0 3.5803282444934553E-4 0.0 0.004251639790335978 0.0 30-31 0.0 4.475410305616819E-4 0.0 0.010293443702918684 0.0 32-33 0.0 4.475410305616819E-4 0.0 0.019244264314152323 0.0 34-35 0.0 4.475410305616819E-4 0.0 0.02797131441010512 0.0 36-37 0.0 4.475410305616819E-4 0.0 0.03929410248331568 0.0 38-39 0.0 4.475410305616819E-4 0.0 0.0510644315870879 0.0 40-41 0.0 4.475410305616819E-4 0.0 0.06722066279036462 0.0 42-43 0.0 4.475410305616819E-4 0.0 0.08825509122676367 0.0 44-45 0.0 4.475410305616819E-4 0.0 0.1067832898920173 0.0 46-47 0.0 5.370492366740182E-4 0.0 0.13130853836679746 0.0 48-49 0.0 5.370492366740182E-4 0.0 0.15619181966602697 0.0 50-51 0.0 5.370492366740182E-4 0.0 0.19029444619482716 0.0 52-53 0.0 5.370492366740182E-4 0.0 0.2661974049780884 0.0 54-55 0.0 6.265574427863547E-4 0.0 0.3729359407670495 0.0 56-57 0.0 7.160656488986911E-4 0.0 0.45823726119210606 0.0 58-59 0.0 7.160656488986911E-4 0.0 0.601092358147395 0.0 60-61 0.0 7.160656488986911E-4 0.0 0.7866876235213245 0.0 62-63 0.0 7.160656488986911E-4 0.0 0.9038538653223728 0.0 64-65 0.0 7.160656488986911E-4 0.0 0.9916166614155186 0.0 66-67 0.0 7.160656488986911E-4 0.0 1.2111802910090796 0.0 68-69 0.0 7.160656488986911E-4 0.0 1.4274321169764845 0.0 70-71 0.0 7.160656488986911E-4 0.0 1.5535939334918225 0.0 72-73 0.0 7.160656488986911E-4 0.0 1.803321828545241 0.0 74-75 0.0 9.398361641795319E-4 0.0 2.139111863775671 0.0 76-77 0.0 0.0010740984733480364 0.0 2.458969438318105 0.0 78-79 0.0 0.0010740984733480364 0.0 2.781422750837797 0.0 80-81 0.0 0.0010740984733480364 0.0 3.1988890241457337 0.0 82-83 0.0 0.0010740984733480364 0.0 3.644774152894337 0.0 84-85 0.0 0.0010740984733480364 0.0 4.178780110560536 0.0 86-87 0.0 0.0010740984733480364 0.0 4.8907731360811155 0.0 88-89 0.0 0.0012531148855727094 0.0 5.58625189757397 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTACG 75 3.0694972E-4 31.682297 1 CATTCCG 110 9.476336E-5 25.909115 9 CATGGGT 1205 0.0 24.439857 4 GTAGCCC 390 0.0 24.358997 3 GAGTACT 7180 0.0 23.4854 12-13 ACCGAGT 125 2.2558335E-4 22.800022 8 AGTACTT 7350 0.0 21.681993 12-13 GTACTTT 7795 0.0 21.661976 14-15 GAACGCA 200 5.385773E-7 21.37502 9 CGGGAGT 250 9.487849E-9 20.90002 4 GTCGGGA 260 1.4899342E-8 20.096172 2 GGGAACG 240 1.4130819E-7 19.791685 7 ACTTTTT 8875 0.0 19.160582 16-17 TACCTGG 880 0.0 18.892063 2 TACTTTT 8510 0.0 18.809399 14-15 GTACATG 11660 0.0 18.748528 1 GTCCATA 280 3.468267E-8 18.669924 1 TACATGG 11735 0.0 18.66001 2 GTGTAGC 515 0.0 18.455706 1 ACATGGG 11495 0.0 18.42977 3 >>END_MODULE