FastQCFastQC Report
Thu 26 May 2016
SRR937379_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937379_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1766065
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80440.45547587433078623No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59640.3376999147822985No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42980.24336590102855782No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32570.18442129819683872No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47350.041.1638681
GTATCAA80450.038.8232231
TCAACGC101150.030.5788124
ATCAACG101600.030.4892753
CAACGCA102550.030.1622125
TATCAAC103100.029.9560762
AACGCAG104200.029.6795376
GTACATG74200.025.7557641
ACGCAGA119600.025.5813357
ATACCGT1301.0016034E-525.5798826
CGCAGAG119850.025.4883338
TACATGG76350.024.5820582
ACATGGG76050.024.0521933
GCAGAGT127300.023.4368789
GAGTACT69200.022.44585612-13
AGTACTT72300.021.45059812-13
AGAGTAC112300.021.25660910-11
GTATAGG5000.020.9172691
GTACTTT74900.020.41973514-15
CATGGGG33600.019.9381734
Produced by FastQC (version 0.11.5)