##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937379_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1766065 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.551250378666698 31.0 31.0 34.0 30.0 34.0 2 31.67846370320458 33.0 31.0 34.0 30.0 34.0 3 31.710228672217614 33.0 31.0 34.0 30.0 34.0 4 35.34126037263634 37.0 35.0 37.0 33.0 37.0 5 35.34177564245937 37.0 35.0 37.0 33.0 37.0 6 35.37081251256324 37.0 35.0 37.0 33.0 37.0 7 35.3003609719914 37.0 35.0 37.0 33.0 37.0 8 35.28079034463624 37.0 35.0 37.0 33.0 37.0 9 36.85447477867462 39.0 37.0 39.0 33.0 39.0 10-11 36.719973500409104 39.0 37.0 39.0 33.0 39.0 12-13 36.5645315999128 39.0 37.0 39.0 32.0 39.0 14-15 37.63001758145934 40.0 37.0 41.0 32.0 41.0 16-17 37.53978873937256 40.0 37.0 41.0 32.0 41.0 18-19 37.45483348574373 40.0 37.0 41.0 32.0 41.0 20-21 37.31324639806576 40.0 37.0 41.0 32.0 41.0 22-23 37.39287172329445 40.0 37.0 41.0 32.0 41.0 24-25 37.349038398926425 40.0 37.0 41.0 32.0 41.0 26-27 37.25819831093419 40.0 37.0 41.0 31.5 41.0 28-29 37.102232080925674 39.5 37.0 41.0 31.0 41.0 30-31 36.82884689974605 39.0 36.0 41.0 30.5 41.0 32-33 36.56882023028598 39.0 35.5 40.5 30.0 41.0 34-35 36.54097895604069 39.0 36.0 41.0 30.0 41.0 36-37 36.373489933835955 39.0 35.0 41.0 30.0 41.0 38-39 36.210380422011646 39.0 35.0 41.0 30.0 41.0 40-41 35.953056937315445 39.0 35.0 40.0 29.0 41.0 42-43 35.660187762058584 39.0 35.0 40.0 27.5 41.0 44-45 35.38157457398227 38.0 35.0 40.0 26.5 41.0 46-47 34.943104302503016 38.0 34.0 40.0 24.5 41.0 48-49 34.56766143941475 38.0 34.0 40.0 24.0 41.0 50-51 33.593717388657836 37.5 32.5 39.5 22.5 40.5 52-53 33.68969403730894 37.5 33.0 39.5 23.0 40.0 54-55 33.95901028557839 38.0 33.0 40.0 22.0 41.0 56-57 33.649541211676805 38.0 33.0 40.0 20.0 41.0 58-59 33.330822195106066 37.5 33.0 40.0 18.5 41.0 60-61 32.95716465701999 37.0 32.0 40.0 16.5 41.0 62-63 32.53136039726737 36.5 31.5 39.5 12.5 41.0 64-65 32.0646751393635 36.0 31.0 39.0 8.0 40.5 66-67 31.595955131889255 35.5 30.5 39.0 7.5 40.0 68-69 31.05523607568238 35.0 30.0 38.5 4.0 40.0 70-71 30.534260630271252 35.0 29.0 38.0 2.0 40.0 72-73 30.000721377752235 34.5 29.0 37.0 2.0 39.0 74-75 29.403124743426773 34.0 28.5 36.5 2.0 39.0 76-77 28.82229589511145 34.0 27.5 36.0 2.0 39.0 78-79 28.214545897234814 34.0 26.0 35.5 2.0 37.5 80-81 27.559709580338208 33.0 26.0 35.0 2.0 37.0 82-83 26.930807756226415 33.0 25.0 35.0 2.0 36.5 84-85 26.26692703835929 32.0 23.5 35.0 2.0 36.0 86-87 25.586803996455394 32.0 20.5 35.0 2.0 36.0 88-89 24.945255412456504 31.0 18.5 34.0 2.0 35.0 90-91 24.286941590485064 31.0 13.5 34.0 2.0 35.0 92-93 23.506093490330198 31.0 4.5 34.0 2.0 35.0 94-95 22.62248643169985 30.0 2.0 34.0 2.0 35.0 96-97 21.667668517296928 29.5 2.0 34.0 2.0 35.0 98-99 20.394451223482715 29.0 2.0 34.0 2.0 35.0 100-101 17.411270819590445 22.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15072164826693069 1101 2 0.24956944159356098 1101 3 0.06849468727201113 1101 4 0.13749455501793761 1101 5 0.06816676290920753 1101 6 0.024580837989269355 1101 7 0.0644974729206993 1101 8 0.010532512157041651 1101 9 0.022954127770830723 1101 10-11 0.1272345149998344 1101 12-13 0.18176744270686385 1101 14-15 0.31986274778772383 1101 16-17 0.24027552680355058 1101 18-19 0.3037076305042561 1101 20-21 0.40479817936547846 1101 22-23 0.35271654647350914 1101 24-25 0.29271950098875976 1101 26-27 0.18651042534417428 1101 28-29 0.16266911485195124 1101 30-31 0.10962950765508594 1101 32-33 0.23124497970322722 1101 34-35 0.37920158397388093 1101 36-37 0.35890987864701884 1101 38-39 0.347409407529355 1101 40-41 0.4808141978849534 1101 42-43 0.5362961830595765 1101 44-45 0.6169557361698494 1101 46-47 0.8249431096951554 1101 48-49 0.7975379385349086 1101 50-51 0.8284368120263039 1101 52-53 0.7090155133787306 1101 54-55 0.7939338394266997 1101 56-57 0.901992822458169 1101 58-59 0.9205458391842143 1101 60-61 0.9530436155875819 1101 62-63 1.1840929238560278 1101 64-65 0.9857951661998143 1101 66-67 1.0988870842466767 1101 68-69 1.23943276602769 1101 70-71 1.176365616669166 1101 72-73 1.2649637712727184 1101 74-75 1.3801797681370616 1101 76-77 1.4941086171599487 1101 78-79 1.4909403958434524 1101 80-81 1.3910971624140842 1101 82-83 1.2283171486022688 1101 84-85 1.4937509837821992 1101 86-87 1.8683907975385736 1101 88-89 1.7298453912122618 1101 90-91 1.8048954176359402 1101 92-93 2.175755347978214 1101 94-95 2.0961122512090995 1101 96-97 1.9303290935574182 1101 98-99 2.1294962143970615 1101 100-101 1.9995417638341664 1102 1 0.19076682863908445 1102 2 0.24642789808201826 1102 3 0.13614880124764994 1102 4 0.11829412258073546 1102 5 0.08062200847788858 1102 6 0.14863258336612262 1102 7 -0.07432982308428393 1102 8 -0.006292457414232899 1102 9 0.2467667334133523 1102 10-11 0.16946670239909878 1102 12-13 0.15972362534538576 1102 14-15 0.485757511595871 1102 16-17 0.5179209258457647 1102 18-19 0.43133482747033014 1102 20-21 0.28363486445485364 1102 22-23 0.21264084544843342 1102 24-25 0.2785356710513014 1102 26-27 0.33672014758172253 1102 28-29 0.3539392275332247 1102 30-31 0.3336164732525475 1102 32-33 0.36704901504835163 1102 34-35 0.5509412366758113 1102 36-37 0.5958537194199138 1102 38-39 0.4794078606593075 1102 40-41 0.5932702401775032 1102 42-43 0.746357641113292 1102 44-45 0.7094587483989514 1102 46-47 0.7151827519783893 1102 48-49 0.8079183312830054 1102 50-51 0.6921887563389788 1102 52-53 0.8108077130843085 1102 54-55 0.8800265952455391 1102 56-57 0.8836090319891952 1102 58-59 1.0285270512096076 1102 60-61 0.9557920065117926 1102 62-63 0.9210042828095482 1102 64-65 0.8055302616412021 1102 66-67 1.0994778856405816 1102 68-69 1.1402335960913916 1102 70-71 1.2870664149864837 1102 72-73 1.2601975239279533 1102 74-75 1.1426558510993061 1102 76-77 1.1881117991525194 1102 78-79 1.3240972241960343 1102 80-81 1.4056422759840075 1102 82-83 1.3366348232472056 1102 84-85 1.4726056258019078 1102 86-87 1.490136946604384 1102 88-89 1.6690948895826558 1102 90-91 1.6465673855806742 1102 92-93 1.6982005915979848 1102 94-95 1.8061255993982677 1102 96-97 1.8773055877517493 1102 98-99 2.131755539437112 1102 100-101 2.0467096556170006 1103 1 0.2415925318767833 1103 2 0.19796468616237206 1103 3 0.1906234265882496 1103 4 0.22331980110827487 1103 5 0.20964617434314192 1103 6 0.17681760567204918 1103 7 0.10141991311810017 1103 8 0.09340008479338735 1103 9 0.21730947196654427 1103 10-11 0.10754522059111338 1103 12-13 0.15492914539400715 1103 14-15 0.0695478993449754 1103 16-17 0.20949893647780726 1103 18-19 0.11702059275056342 1103 20-21 0.06692011315924162 1103 22-23 0.00721596432432392 1103 24-25 -0.007338300471907644 1103 26-27 0.0909726319542159 1103 28-29 -0.05259460534598048 1103 30-31 0.19787152046898626 1103 32-33 0.20959982113372888 1103 34-35 0.1802897233456875 1103 36-37 0.23133341368181704 1103 38-39 0.2542841429160845 1103 40-41 0.36549831993120563 1103 42-43 0.17725163955054057 1103 44-45 0.36032510652258054 1103 46-47 0.331901301625777 1103 48-49 0.31188948718405385 1103 50-51 0.3804795230884608 1103 52-53 0.1517978557353743 1103 54-55 0.49343890925152323 1103 56-57 0.3434185049950145 1103 58-59 0.3024700241753564 1103 60-61 0.31990158937763624 1103 62-63 0.4929690658276584 1103 64-65 0.27836265498682167 1103 66-67 0.660131928422544 1103 68-69 0.6697325976674975 1103 70-71 0.6155587281242738 1103 72-73 0.7343313745049684 1103 74-75 0.754669870773931 1103 76-77 0.5814284113900534 1103 78-79 0.4712829377185024 1103 80-81 0.5035650367095492 1103 82-83 0.6753634856931079 1103 84-85 0.6507249491443226 1103 86-87 0.7401081459151726 1103 88-89 0.8889115585867664 1103 90-91 0.7223826955261785 1103 92-93 1.075953353124941 1103 94-95 1.001730280303331 1103 96-97 1.1444591112865083 1103 98-99 1.209860504175051 1103 100-101 1.0253447617371023 1104 1 0.010725767307192768 1104 2 0.13838516331987094 1104 3 0.12986838719992733 1104 4 0.08887904032759764 1104 5 0.08442342247595036 1104 6 0.14101418270416133 1104 7 0.16996658282862143 1104 8 0.16411038640708142 1104 9 0.08885064230400275 1104 10-11 0.10344028274015926 1104 12-13 0.14578545808822696 1104 14-15 0.38510211280514994 1104 16-17 0.23014458468149002 1104 18-19 0.19971541332243703 1104 20-21 0.12697026853186344 1104 22-23 0.16809818097372187 1104 24-25 0.05338564735559714 1104 26-27 0.24240471776351313 1104 28-29 0.264394504941329 1104 30-31 0.1851569886993758 1104 32-33 0.23988683457209703 1104 34-35 0.25535667667938355 1104 36-37 0.2873659431208395 1104 38-39 0.2732653278367678 1104 40-41 0.3063985867223522 1104 42-43 0.35390941601840353 1104 44-45 0.2967288095700624 1104 46-47 0.31490294905258054 1104 48-49 0.2959987182258672 1104 50-51 0.24648958993957848 1104 52-53 0.3909524378648044 1104 54-55 0.511782291263728 1104 56-57 0.5067465201097718 1104 58-59 0.5962784592732078 1104 60-61 0.5627875847164532 1104 62-63 0.447920162635711 1104 64-65 0.6524658977088436 1104 66-67 0.8128210928858266 1104 68-69 0.8166674808654477 1104 70-71 0.5649442225908281 1104 72-73 0.8225856216973568 1104 74-75 0.8112712054111242 1104 76-77 0.9130299163919666 1104 78-79 0.8913273688366417 1104 80-81 1.0631334858454622 1104 82-83 1.0715253389886463 1104 84-85 1.010476455168419 1104 86-87 1.0982007255095638 1104 88-89 1.1083215089084177 1104 90-91 1.2668864316244175 1104 92-93 1.4089648391141445 1104 94-95 1.4399206833181033 1104 96-97 1.678017553198952 1104 98-99 1.796778027208358 1104 100-101 1.5854820160711824 1105 1 0.08286993624343708 1105 2 -0.09139189678463211 1105 3 -0.035747355620340926 1105 4 0.07762685840500438 1105 5 0.10768115373131337 1105 6 0.03138643149166853 1105 7 0.06363090505381308 1105 8 0.06597077747584024 1105 9 0.08021108170198943 1105 10-11 0.25047343659200294 1105 12-13 0.15253733582095919 1105 14-15 0.2606642954668956 1105 16-17 0.21650010586959212 1105 18-19 0.32467774495192714 1105 20-21 0.3572832145111491 1105 22-23 0.4013478384047673 1105 24-25 0.35913199281293373 1105 26-27 0.4330379443934902 1105 28-29 0.3130407179197121 1105 30-31 0.32443898691403206 1105 32-33 0.2786440342059322 1105 34-35 0.23478832493356094 1105 36-37 0.38631577738000544 1105 38-39 0.36123447673058706 1105 40-41 0.11284712746389403 1105 42-43 0.19186719332868307 1105 44-45 0.2639686988108423 1105 46-47 0.3517639371294763 1105 48-49 0.3300935686042621 1105 50-51 0.3496980822381701 1105 52-53 0.26771858693488326 1105 54-55 0.3721250307919277 1105 56-57 0.45866746486093746 1105 58-59 0.5975961415170516 1105 60-61 0.6111011788760052 1105 62-63 0.63276809613469 1105 64-65 0.653695450180173 1105 66-67 0.83856209369889 1105 68-69 0.5381944571045238 1105 70-71 0.773196296483377 1105 72-73 0.8977418388263736 1105 74-75 0.7762026631912775 1105 76-77 0.609139981949145 1105 78-79 0.685351033102009 1105 80-81 0.7893071754126026 1105 82-83 0.9937863509458644 1105 84-85 0.938087797185851 1105 86-87 1.0078847660606343 1105 88-89 1.1746789632657233 1105 90-91 1.2656611434969207 1105 92-93 1.4419779676073539 1105 94-95 1.3432579588004003 1105 96-97 1.398152931901116 1105 98-99 1.36054418196424 1105 100-101 1.1591144410045189 1106 1 0.009990731673944708 1106 2 -0.6921584967314729 1106 3 -0.1414227898868461 1106 4 -0.058630982078561544 1106 5 -0.027537240689532894 1106 6 -0.11839347444965398 1106 7 -0.04903541987716409 1106 8 -0.07315565388139333 1106 9 -0.03468194960208848 1106 10-11 -0.4393509085502032 1106 12-13 -0.27662212094585925 1106 14-15 -0.25135973813530654 1106 16-17 -0.1426005958638612 1106 18-19 0.04071357928425812 1106 20-21 0.0562710259381447 1106 22-23 0.13972385253708097 1106 24-25 0.15697950998561083 1106 26-27 0.1979213022269235 1106 28-29 0.16807110346628207 1106 30-31 0.10821793274078573 1106 32-33 0.012192014620318048 1106 34-35 0.16000222616290216 1106 36-37 0.10656759499801893 1106 38-39 0.18770269711937715 1106 40-41 0.1513307024531798 1106 42-43 0.09828235427895748 1106 44-45 0.08946007947728418 1106 46-47 0.30755436816848203 1106 48-49 0.14350079550048633 1106 50-51 0.19510212140939842 1106 52-53 0.13140119132248884 1106 54-55 0.21021949720509525 1106 56-57 0.15899543619853063 1106 58-59 -0.05408071393598135 1106 60-61 -0.005129752181979086 1106 62-63 0.16897569333498552 1106 64-65 0.1442551147005915 1106 66-67 0.3088995712555338 1106 68-69 0.2634033633875781 1106 70-71 0.21720046240419322 1106 72-73 0.2941928729836718 1106 74-75 0.25040794689518364 1106 76-77 0.30837211631455475 1106 78-79 0.4404484774976325 1106 80-81 0.1907533818752043 1106 82-83 0.4050548593690202 1106 84-85 0.2681219797956622 1106 86-87 0.39379623753112725 1106 88-89 0.6039718822852116 1106 90-91 0.616285353572156 1106 92-93 0.8426682646642654 1106 94-95 0.7903666759535426 1106 96-97 0.7672475944958812 1106 98-99 0.5956891890972962 1106 100-101 0.01838182929401455 1107 1 0.05303496029564769 1107 2 0.03617697978934231 1107 3 -0.05479947576700184 1107 4 0.1647930231252488 1107 5 0.015778646345687264 1107 6 0.015700972438779104 1107 7 0.08477702865813797 1107 8 0.018593920301093192 1107 9 -0.0074084788791566325 1107 10-11 -0.048366536898925006 1107 12-13 -0.016372420804110277 1107 14-15 -0.17354699256174655 1107 16-17 -0.06818734043726948 1107 18-19 0.011439095247240516 1107 20-21 0.12686931364413567 1107 22-23 0.07676584021186983 1107 24-25 -0.06229864942994112 1107 26-27 -0.09332894687162252 1107 28-29 0.013064128707007683 1107 30-31 -0.05508449357303391 1107 32-33 -0.1282697200831464 1107 34-35 -0.008396380389228852 1107 36-37 -0.022572610823182515 1107 38-39 0.0397130163791104 1107 40-41 0.057458385667516154 1107 42-43 -0.15309967265000068 1107 44-45 -0.0702503928135556 1107 46-47 0.031493020366752944 1107 48-49 -0.0849411353986298 1107 50-51 0.011906356272668006 1107 52-53 -0.14012256797875722 1107 54-55 0.07725562871408442 1107 56-57 0.2872560421565922 1107 58-59 0.2724385991912328 1107 60-61 0.24432152602255996 1107 62-63 0.2329171749167358 1107 64-65 -0.12534353584328528 1107 66-67 0.2559896302495872 1107 68-69 0.31750263244784804 1107 70-71 0.31662180178357247 1107 72-73 0.5411536357328153 1107 74-75 0.4962221513402305 1107 76-77 0.6645506062321722 1107 78-79 0.6433446475839979 1107 80-81 0.48845551829472456 1107 82-83 0.5911465125937632 1107 84-85 0.25546918368799254 1107 86-87 0.362461285527246 1107 88-89 0.27935864302653 1107 90-91 0.49501901176759233 1107 92-93 0.643043980470086 1107 94-95 0.5006086681403055 1107 96-97 0.38303282831011387 1107 98-99 0.6344703076155618 1107 100-101 0.6394030675299831 1108 1 -0.060772661257171734 1108 2 -0.10120766628413236 1108 3 -0.03529335020175495 1108 4 -0.027459748457822286 1108 5 0.03740215810378089 1108 6 -0.007523783154020691 1108 7 0.003650484887899097 1108 8 -0.177241195204914 1108 9 -0.14014006268874368 1108 10-11 -0.13235155202951177 1108 12-13 0.003504954712205688 1108 14-15 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10-11 0.3133948776476103 2315 12-13 0.3769242119482996 2315 14-15 0.3651350447762667 2315 16-17 0.4447894212487924 2315 18-19 0.30504826800050466 2315 20-21 0.5374792854447534 2315 22-23 0.5854971779955562 2315 24-25 0.41746479764480426 2315 26-27 0.390328886313668 2315 28-29 0.5826573709386054 2315 30-31 0.421697556565249 2315 32-33 0.5059498705408458 2315 34-35 0.4388306149987926 2315 36-37 0.5785437281435648 2315 38-39 0.5635763133415708 2315 40-41 0.5286335283129802 2315 42-43 0.45710389048056044 2315 44-45 0.4346937126606889 2315 46-47 0.6203089757132005 2315 48-49 0.737876902901327 2315 50-51 0.8106002435604367 2315 52-53 0.756555470243633 2315 54-55 0.7752220338155809 2315 56-57 0.8709547974270038 2315 58-59 0.9101739011807055 2315 60-61 0.7120978082802125 2315 62-63 1.0510015364740184 2315 64-65 1.23754275118538 2315 66-67 1.214520624365349 2315 68-69 1.6208468007598071 2315 70-71 1.5344111752295966 2315 72-73 1.3031117090202002 2315 74-75 1.5027798669957413 2315 76-77 1.772513151857389 2315 78-79 1.6399065136310824 2315 80-81 1.706611929873933 2315 82-83 1.821757492900737 2315 84-85 2.2624467870889866 2315 86-87 2.398597814015613 2315 88-89 2.5089426923347347 2315 90-91 2.6762951214155066 2315 92-93 2.941705867075182 2315 94-95 3.2099939835118825 2315 96-97 3.3510825709470495 2315 98-99 3.550504395130641 2315 100-101 3.8116035896259675 2316 1 -0.07459427643549787 2316 2 -0.15093256061977556 2316 3 -0.20650400183710715 2316 4 -0.2558637818341012 2316 5 -0.18687602732893538 2316 6 -0.18310153453004574 2316 7 -0.22699360336211072 2316 8 0.02876769426048753 2316 9 -0.27522387189247155 2316 10-11 -0.2104751240498146 2316 12-13 -0.12853077806924063 2316 14-15 -0.13169845425782256 2316 16-17 -0.13838427355466365 2316 18-19 0.17590473881696056 2316 20-21 -0.23143788034491308 2316 22-23 -0.2357844567029872 2316 24-25 0.16584113234416265 2316 26-27 -0.15096394328937635 2316 28-29 0.22290386091168557 2316 30-31 0.1545475286790179 2316 32-33 0.4939971882093914 2316 34-35 0.19644476665135358 2316 36-37 0.10977023703078004 2316 38-39 0.2686986894095327 2316 40-41 0.20141091541418632 2316 42-43 0.12627021362127522 2316 44-45 0.14500535131988812 2316 46-47 0.0651114280843359 2316 48-49 0.08477344905101347 2316 50-51 -0.06515955501210868 2316 52-53 0.10950716645353253 2316 54-55 -0.06274991021798826 2316 56-57 -0.09430563565817351 2316 58-59 0.1305912650029768 2316 60-61 0.01652040177989278 2316 62-63 0.3911053621406637 2316 64-65 0.5245071538139641 2316 66-67 0.4389238995821678 2316 68-69 0.7672333119102497 2316 70-71 0.8985699003845902 2316 72-73 0.6098308745350423 2316 74-75 0.6874422416087675 2316 76-77 0.8426227568429603 2316 78-79 0.8270059017507378 2316 80-81 0.9965983504951872 2316 82-83 1.2147239001042713 2316 84-85 1.2863652865676336 2316 86-87 1.472215425176568 2316 88-89 1.4907085369640107 2316 90-91 1.7716781326427409 2316 92-93 1.9697426364763153 2316 94-95 2.113786510003795 2316 96-97 2.1308884989925083 2316 98-99 2.3521282625053743 2316 100-101 3.094186015646642 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8541.0 3 1760.0 4 1735.0 5 1862.0 6 2210.0 7 2744.0 8 3208.0 9 4218.0 10 5172.0 11 6556.0 12 8620.0 13 10733.0 14 12223.0 15 14401.0 16 17751.0 17 22165.0 18 20069.0 19 15888.0 20 16284.0 21 17652.0 22 19200.0 23 21256.0 24 24257.0 25 27521.0 26 31450.0 27 36227.0 28 42253.0 29 49758.0 30 59071.0 31 70401.0 32 85925.0 33 107351.0 34 137939.0 35 179703.0 36 232778.0 37 267093.0 38 165483.0 39 14607.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.65350011221496 19.419266313071194 15.290153777098492 22.63707979761536 2 14.7457372045148 20.08717257045503 41.23864555973593 23.928444665294244 3 18.598800626945007 24.941223905685664 30.232662471889693 26.227312995479636 4 12.091184619779193 16.73310008285484 38.064558629027815 33.11115666833815 5 13.30764945365913 37.67318588196812 34.44698923332824 14.572175431044517 6 31.128946282364012 36.67720087217999 17.86050229256632 14.333350552889678 7 27.94879684274824 33.10192491535776 21.021247414182653 17.928030827711332 8 26.137113901285215 34.96851261340483 19.990587562070715 18.90378592323924 9 26.19245118261475 16.79290800194628 20.637662102447667 36.3769787129913 10-11 24.453391112875085 27.008557345457046 27.675724813576373 20.8623267280915 12-13 25.4467497060465 24.39033391187243 28.31699059746226 21.845925784618807 14-15 22.332751451157378 25.57596400845858 26.802656788861924 25.28862775152212 16-17 21.563050454327936 29.117178404110323 27.231491820280617 22.08827932128112 18-19 21.43815756725409 27.277407285376203 30.069821712415596 21.214613434954106 20-21 22.138416329737083 26.395194985874788 30.17074567629898 21.29564300808915 22-23 21.848984185960976 26.409178397640126 30.09304746482127 21.64878995157763 24-25 21.62458858251012 26.62040380997358 30.139103598734096 21.615904008782206 26-27 21.522283342712836 26.832784611145122 29.937868244807003 21.70706380133504 28-29 21.249260575893583 26.8312455364898 30.262911395135756 21.656582492480865 30-31 21.438213500661035 26.595361431297977 30.187091076275465 21.77933399176553 32-33 21.25929513127581 26.800635401491764 30.046990869793788 21.893078597438638 34-35 21.76794319219188 26.81538143618205 29.761934499343724 21.65474087228235 36-37 21.772941774491418 26.617938932056003 29.73721024684487 21.87190904660771 38-39 21.472271118641935 26.91171600467552 29.83814189379972 21.77787098288283 40-41 21.658768444948922 26.714869466515324 29.914443813847903 21.711918274687854 42-43 21.53074890783033 26.786019887662547 29.62913769327068 22.05409351123644 44-45 21.619891208071728 26.990893138763774 29.38341307642992 22.005802576734578 46-47 21.72411913904172 27.244614469348967 28.89183638250381 22.139430009105503 48-49 21.85401439134816 27.316339999148443 28.722576250017738 22.10706935948566 50-51 21.853880861122175 27.38745189041533 28.520477500314414 22.23818974814808 52-53 22.028160759782285 27.49971534854825 28.44657539458654 22.025548497082927 54-55 21.812890197661357 27.372552225528217 28.5043489812637 22.31020859554673 56-57 21.860451257544845 27.671066110847832 28.391511749665852 22.076970881941467 58-59 21.98872037196612 27.28651940595964 28.49948941765239 22.22527080442185 60-61 21.953393271511093 27.309410271208623 28.8153271942384 21.921869263041888 62-63 21.844133157472967 27.409841294344396 28.783362580334664 21.962662967847972 64-65 21.733829494140128 27.317057253798172 28.698088608115718 22.251024643945982 66-67 21.66138881322074 27.587226459638607 28.40291019703986 22.34847453010079 68-69 21.672165905626475 27.478885532972164 28.624730571341985 22.224217990059373 70-71 21.68030838948218 27.42606299737665 28.602358322585953 22.291270290555218 72-73 21.796567863776982 27.37351964508372 28.443983602659035 22.38592888848026 74-75 21.652635659398694 27.596966055991977 28.325371424874184 22.42502685973514 76-77 21.732642018813987 27.423668931944047 28.34166256070951 22.502026488532458 78-79 21.82741577778926 27.514121471618836 28.261618603616213 22.3968441469757 80-81 21.713653428490264 27.7740333164667 28.251908563754867 22.260404691288162 82-83 21.804833449015415 27.661260160963625 28.181091739958784 22.35281465006217 84-85 21.82997549466753 27.448582637332148 28.104129362733683 22.617312505266646 86-87 21.832942909610097 27.364759038195697 28.324560149087386 22.47773790310682 88-89 21.903316018507617 27.24648867536617 28.201778967098047 22.648416339028167 90-91 21.741198250731344 27.274816848075773 28.401353353460156 22.582631547732724 92-93 21.877720756584583 26.954716394293037 28.437872818144772 22.729690030977608 94-95 21.723860134113387 26.913993143651037 28.403990541518766 22.95815618071681 96-97 21.72946057337651 26.855998612830806 28.349419066141124 23.06512174765156 98-99 21.520704539255995 26.96021712187506 28.182648697294294 23.33642964157465 100-101 21.965046965727957 26.07548452559848 28.910708367572084 23.048760141101482 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3082.0 1 1749.5 2 419.5 3 444.0 4 391.0 5 357.0 6 588.0 7 1267.5 8 2156.5 9 2517.0 10 2240.0 11 1960.0 12 2023.5 13 2246.0 14 2405.0 15 2533.0 16 2691.5 17 2865.0 18 3041.5 19 3213.0 20 3527.0 21 4042.5 22 4881.5 23 6003.5 24 7259.5 25 8899.0 26 11138.5 27 13806.5 28 16171.0 29 18895.0 30 22380.0 31 25792.0 32 29435.5 33 33210.0 34 37806.5 35 41593.5 36 45351.0 37 50122.0 38 52419.5 39 53328.5 40 54733.0 41 57252.5 42 60065.0 43 62434.0 44 64357.0 45 65988.5 46 66091.0 47 66079.5 48 68870.5 49 68790.0 50 65262.0 51 61460.0 52 57645.0 53 55185.0 54 52705.0 55 48593.5 56 43707.0 57 38879.5 58 34770.5 59 30803.0 60 26438.5 61 21829.0 62 17085.5 63 13247.5 64 10386.0 65 8089.0 66 5984.0 67 4595.0 68 3409.0 69 2241.5 70 1674.5 71 1275.0 72 949.5 73 720.0 74 527.5 75 411.0 76 290.0 77 166.5 78 105.5 79 77.5 80 52.0 81 21.0 82 19.0 83 17.0 84 12.0 85 11.5 86 8.0 87 9.0 88 11.0 89 7.5 90 3.0 91 1.0 92 2.0 93 3.0 94 2.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34256949772516865 2 0.293873668296467 3 0.29132563071008144 4 0.2918352382273586 5 0.2926279610320119 6 0.2549170047535057 7 0.25667231953523795 8 0.2585408804319207 9 0.2692992613522152 10-11 0.2767735049389462 12-13 0.2679403079728096 14-15 0.2570686809375646 16-17 0.2520858518797439 18-19 0.23897761407422718 20-21 0.24472485440796346 22-23 0.24212019376410268 24-25 0.24463991982175062 26-27 0.2403365674536328 28-29 0.258144519029594 30-31 0.2723852179846155 32-33 0.2638068247771175 34-35 0.26142865636315765 36-37 0.24752769575298758 38-39 0.23331530832670372 40-41 0.2301727286368282 42-43 0.2445832967642754 44-45 0.24129915943071176 46-47 0.25440739723622857 48-49 0.2598998338113263 50-51 0.2747067633411001 52-53 0.29101420389396765 54-55 0.2614852794206329 56-57 0.2321545356484614 58-59 0.21864993644061798 60-61 0.22173589307301825 62-63 0.22994623640692724 64-65 0.21898967478546938 66-67 0.22564288403880947 68-69 0.22666209907336365 70-71 0.22691690283200222 72-73 0.23056909003915488 74-75 0.2340797196026194 76-77 0.24897158371860606 78-79 0.24834873008637848 80-81 0.23801502209714817 82-83 0.22943662888965016 84-85 0.21491281464725254 86-87 0.21952759383148412 88-89 0.231446747430021 90-91 0.23054077851041724 92-93 0.23481581934979745 94-95 0.22923844818848682 96-97 0.23801502209714817 98-99 0.24288460504001833 100-101 0.2224719928201963 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1766065.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.91544207984091 #Duplication Level Percentage of deduplicated Percentage of total 1 77.97604965342772 44.38041337664465 2 12.492382188706056 14.220189098010716 3 4.087178095095488 6.978706444242053 4 1.7984922128845136 4.094479174938938 5 0.9667673318724485 2.751199504093434 6 0.5768462801886182 1.9698876629428188 7 0.3881274462304351 1.5463311629867427 8 0.25984776130397985 1.1831480166458392 9 0.19663963000787366 1.0072648325083033 >10 1.1181780033163429 11.788903779106109 >50 0.08939808905053656 3.5145007282156873 >100 0.048300972446853295 4.786687939196503 >500 0.001194890312770209 0.4167172454039149 >1k 3.9829677092340294E-4 0.5676943624657984 >5k 1.9914838546170147E-4 0.7938766725986727 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8044 0.45547587433078623 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5964 0.3376999147822985 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4298 0.24336590102855782 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3257 0.18442129819683872 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 2.8311528737617244E-5 0.0 24-25 0.0 0.0 0.0 1.6986917242570347E-4 0.0 26-27 0.0 0.0 0.0 2.548037586385552E-4 0.0 28-29 0.0 0.0 0.0 8.493458621285174E-4 0.0 30-31 0.0 0.0 0.0 0.0020384300691084416 0.0 32-33 0.0 0.0 0.0 0.0032841373335636007 0.0 34-35 0.0 0.0 0.0 0.005294255873934424 0.0 36-37 0.0 0.0 0.0 0.008040474161483298 0.0 38-39 0.0 0.0 0.0 0.013306418506680105 0.0 40-41 0.0 0.0 0.0 0.018912101196728322 0.0 42-43 0.0 0.0 0.0 0.0234702573234847 0.0 44-45 0.0 0.0 0.0 0.02746218287548873 0.0 46-47 0.0 0.0 0.0 0.032784750278160774 0.0 48-49 0.0 0.0 0.0 0.03810731768083281 0.0 50-51 0.0 0.0 0.0 0.04359975425593056 0.0 52-53 0.0 0.0 0.0 0.05684954970513543 0.0 54-55 0.0 0.0 0.0 0.07952708422396684 0.0 56-57 0.0 0.0 0.0 0.11355754176658277 0.0 58-59 0.0 0.0 0.0 0.15905416844793369 0.0 60-61 0.0 0.0 0.0 0.2113172504975751 0.0 62-63 0.0 0.0 0.0 0.24452667370680015 0.0 64-65 0.0 5.662305747523449E-5 0.0 0.28603137483614705 0.0 66-67 0.0 5.662305747523449E-5 0.0 0.36190627185296126 0.0 68-69 0.0 5.662305747523449E-5 0.0 0.4652150402165266 0.0 70-71 0.0 5.662305747523449E-5 0.0 0.5802164699487278 0.0 72-73 0.0 5.662305747523449E-5 0.0 0.7217458021080765 0.0 74-75 0.0 5.662305747523449E-5 0.0 0.862312542290346 0.0 76-77 0.0 5.662305747523449E-5 0.0 0.9910733749890293 0.0 78-79 0.0 5.662305747523449E-5 0.0 1.1219858838717713 0.0 80-81 0.0 5.662305747523449E-5 0.0 1.2662331227899313 0.0 82-83 0.0 5.662305747523449E-5 0.0 1.4321103696636308 0.0 84-85 0.0 5.662305747523449E-5 0.0 1.6789869002556532 0.0 86-87 0.0 5.662305747523449E-5 0.0 1.9928201963121404 0.0 88-89 0.0 5.662305747523449E-5 0.0 2.353650630073072 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4735 0.0 41.163868 1 GTATCAA 8045 0.0 38.823223 1 TCAACGC 10115 0.0 30.578812 4 ATCAACG 10160 0.0 30.489275 3 CAACGCA 10255 0.0 30.162212 5 TATCAAC 10310 0.0 29.956076 2 AACGCAG 10420 0.0 29.679537 6 GTACATG 7420 0.0 25.755764 1 ACGCAGA 11960 0.0 25.581335 7 ATACCGT 130 1.0016034E-5 25.579882 6 CGCAGAG 11985 0.0 25.488333 8 TACATGG 7635 0.0 24.582058 2 ACATGGG 7605 0.0 24.052193 3 GCAGAGT 12730 0.0 23.436878 9 GAGTACT 6920 0.0 22.445856 12-13 AGTACTT 7230 0.0 21.450598 12-13 AGAGTAC 11230 0.0 21.256609 10-11 GTATAGG 500 0.0 20.917269 1 GTACTTT 7490 0.0 20.419735 14-15 CATGGGG 3360 0.0 19.938173 4 >>END_MODULE