FastQCFastQC Report
Thu 26 May 2016
SRR937379_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937379_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1766065
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94190.5333325783592336No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75390.4268812303057928No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53090.3006118121360199No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18750.10616823276606467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55800.045.8147771
GTATCAA90850.035.6711351
ATCAACG115250.027.7782123
TCAACGC116550.027.4683744
TATCAAC116750.027.4620062
CAACGCA118350.027.0506065
AACGCAG121000.026.4174216
TAGGCCG908.9504535E-426.3883865
TACCGTC1401.778496E-523.7529097
GTACATG77000.023.5738321
ACGCAGA139400.022.9350197
CGCAGAG140150.022.7783878
GAGTACT84100.022.45370112-13
TACATGG80700.022.1309282
ACATGGG77950.021.9367223
GCAGAGT145800.021.4069429
GTACTTT89200.021.05922514-15
AGTACTT87900.020.91553312-13
AGAGTAC129700.020.5663210-11
CATGGGG31850.020.2821884
Produced by FastQC (version 0.11.5)