##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937379_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1766065 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.222012213593494 33.0 31.0 34.0 31.0 34.0 2 32.421847440496244 34.0 31.0 34.0 31.0 34.0 3 32.48664347008746 34.0 31.0 34.0 31.0 34.0 4 36.12339409931118 37.0 35.0 37.0 35.0 37.0 5 36.05173875253742 37.0 35.0 37.0 35.0 37.0 6 36.0888268551837 37.0 35.0 37.0 35.0 37.0 7 36.02955836846322 37.0 35.0 37.0 35.0 37.0 8 35.999631950126414 37.0 35.0 37.0 35.0 37.0 9 37.69757511756362 39.0 37.0 39.0 35.0 39.0 10-11 37.61743225758961 39.0 37.0 39.0 35.0 39.0 12-13 37.504241633235466 39.0 37.0 39.0 35.0 39.0 14-15 38.7222701316203 40.0 38.0 41.0 34.5 41.0 16-17 38.6690620107414 40.0 38.0 41.0 34.5 41.0 18-19 38.65620489619578 40.0 38.0 41.0 34.0 41.0 20-21 38.55908502801426 40.0 38.0 41.0 34.0 41.0 22-23 38.49967951349469 40.0 38.0 41.0 34.0 41.0 24-25 38.536122679516325 40.0 38.0 41.0 34.0 41.0 26-27 38.38382873790036 40.0 38.0 41.0 34.0 41.0 28-29 38.23107416771184 40.0 38.0 41.0 34.0 41.0 30-31 38.27183087825193 40.0 38.0 41.0 34.0 41.0 32-33 38.17238889848335 40.0 38.0 41.0 34.0 41.0 34-35 38.03892297282376 40.0 37.0 41.0 33.5 41.0 36-37 37.86760028651267 40.0 37.0 41.0 33.0 41.0 38-39 37.75535328541135 40.0 37.0 41.0 33.0 41.0 40-41 37.559577648614294 40.0 37.0 41.0 33.0 41.0 42-43 37.35190380874996 40.0 37.0 41.0 32.5 41.0 44-45 37.041136934371046 40.0 36.0 41.0 31.5 41.0 46-47 36.66589395067565 39.0 36.0 41.0 31.0 41.0 48-49 36.32045762755051 39.0 36.0 41.0 30.0 41.0 50-51 36.067517899964045 39.0 35.0 40.5 29.5 41.0 52-53 35.82061617211144 39.0 35.0 40.0 29.0 41.0 54-55 35.59128882572272 39.0 35.0 40.0 28.0 41.0 56-57 35.28854289055046 38.0 35.0 40.0 28.0 41.0 58-59 35.01825470749944 38.0 34.0 40.0 27.0 41.0 60-61 34.670710307944496 38.0 34.0 40.0 26.0 41.0 62-63 34.29123984677801 37.0 34.0 40.0 26.0 41.0 64-65 33.86359618700331 37.0 33.0 39.5 25.5 41.0 66-67 33.41925552004031 36.0 33.0 39.0 24.5 40.0 68-69 32.9607347407938 35.5 32.0 39.0 23.0 40.0 70-71 32.52009750490497 35.0 32.0 38.0 22.0 40.0 72-73 32.035497844077085 35.0 31.0 37.5 21.0 39.5 74-75 31.541268866094963 35.0 31.0 37.0 19.5 39.0 76-77 30.240495112014564 33.5 29.5 35.5 18.5 38.0 78-79 30.893663596753235 34.0 31.0 36.0 19.0 38.0 80-81 30.701344514499752 34.0 31.0 36.0 18.0 37.0 82-83 30.291255418118812 34.0 30.0 35.0 15.0 37.0 84-85 29.84308278574118 34.0 30.0 35.0 9.0 36.5 86-87 29.43598055564206 34.0 29.0 35.0 6.5 36.0 88-89 28.96621387095039 34.0 29.0 35.0 2.0 36.0 90-91 28.39326695223562 33.5 29.0 35.0 2.0 35.0 92-93 27.86446903143429 33.0 27.0 35.0 2.0 35.0 94-95 27.343752919626404 33.0 26.5 35.0 2.0 35.0 96-97 26.63822735856268 32.5 25.0 34.0 2.0 35.0 98-99 25.611034984556063 32.0 24.0 34.0 2.0 35.0 100-101 22.626512047971055 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14673197173953412 1101 2 0.1986760251792603 1101 3 0.1431019460971399 1101 4 0.09875924920063284 1101 5 0.1397957480536931 1101 6 0.1168835112585711 1101 7 0.11347669213050438 1101 8 0.07580476377862055 1101 9 0.16638326989476582 1101 10-11 0.12212118211142808 1101 12-13 0.13568679723556443 1101 14-15 0.2833259335808336 1101 16-17 0.17002745801717367 1101 18-19 0.14565285465261724 1101 20-21 0.12624174320080783 1101 22-23 0.15979866971933632 1101 24-25 0.0613152509093311 1101 26-27 0.09217118612242814 1101 28-29 -0.010858658745711125 1101 30-31 0.21516475923822753 1101 32-33 0.20363799239805758 1101 34-35 0.23982429718473242 1101 36-37 0.23881718842666544 1101 38-39 0.20313920491732773 1101 40-41 0.21972968614554134 1101 42-43 0.26112496815245123 1101 44-45 0.15584899381671846 1101 46-47 0.2286011830099497 1101 48-49 0.2686069581537467 1101 50-51 0.3730717019916199 1101 52-53 0.26627232037296267 1101 54-55 0.1980156615036961 1101 56-57 0.24635733417084538 1101 58-59 0.2502936548324257 1101 60-61 0.27838084815348907 1101 62-63 0.2574850704626428 1101 64-65 0.2954028993055289 1101 66-67 0.2903801437528699 1101 68-69 0.34273671840259823 1101 70-71 0.38102513678794736 1101 72-73 0.4015088378868512 1101 74-75 0.4038953692410949 1101 76-77 0.48412991586550547 1101 78-79 0.4795956608130112 1101 80-81 0.3967846717965351 1101 82-83 0.18486579957301785 1101 84-85 0.3264133921625465 1101 86-87 0.5630269337311127 1101 88-89 0.7681986558517799 1101 90-91 0.8954005959846754 1101 92-93 0.9161665175159968 1101 94-95 0.9333675638901262 1101 96-97 1.1465542714372248 1101 98-99 1.5731184518706094 1101 100-101 2.2706956775497567 1102 1 0.18462232047170346 1102 2 0.20185801774889 1102 3 0.11566890460989043 1102 4 0.07027575793211582 1102 5 0.13152943145524176 1102 6 0.12374085233360432 1102 7 0.15700233009182796 1102 8 0.10648966439038077 1102 9 0.19585286017031933 1102 10-11 0.13680204517175554 1102 12-13 0.09792913367121514 1102 14-15 0.21519531744361586 1102 16-17 0.2571672928624409 1102 18-19 0.2947106980314089 1102 20-21 0.24108397348832256 1102 22-23 0.22356354547331847 1102 24-25 0.0989239883961659 1102 26-27 0.1510175176992945 1102 28-29 0.1122140355660477 1102 30-31 0.1020425332162418 1102 32-33 0.1725945660441468 1102 34-35 0.27640893806451317 1102 36-37 0.3278949008730834 1102 38-39 0.36226059008918554 1102 40-41 0.3693011099891592 1102 42-43 0.30979100724585606 1102 44-45 0.19121342144415365 1102 46-47 0.252081248365819 1102 48-49 0.359020408813862 1102 50-51 0.32235716325183716 1102 52-53 0.3568806619493259 1102 54-55 0.21115425100826712 1102 56-57 0.27552447580750794 1102 58-59 0.40512345622754964 1102 60-61 0.49775784685789404 1102 62-63 0.5166002044164415 1102 64-65 0.44279405763171553 1102 66-67 0.5803931487379117 1102 68-69 0.4974283188492521 1102 70-71 0.43864267500924115 1102 72-73 0.5595650645290462 1102 74-75 0.3192863584166794 1102 76-77 0.3555828092601736 1102 78-79 0.41027210389444235 1102 80-81 0.4573908438325063 1102 82-83 0.5669405543352823 1102 84-85 0.6584137451725063 1102 86-87 0.8140010818800754 1102 88-89 0.9422347118970187 1102 90-91 1.073123951393388 1102 92-93 0.9856607572024032 1102 94-95 1.1562878387966364 1102 96-97 1.2288435900667096 1102 98-99 1.5151528066039468 1102 100-101 2.2036651532196494 1103 1 0.14334926322428032 1103 2 0.11313937293401466 1103 3 0.1059618201412249 1103 4 0.06409821879657329 1103 5 0.026514187960025026 1103 6 0.0687106658721035 1103 7 0.028165994808389883 1103 8 0.05765663492518769 1103 9 0.14388514699513166 1103 10-11 0.16821670998182015 1103 12-13 0.11013332137491005 1103 14-15 0.12705164290976967 1103 16-17 0.17848443603443087 1103 18-19 0.1644639637791272 1103 20-21 0.21571307069941525 1103 22-23 0.1743356543850041 1103 24-25 0.22247331020254535 1103 26-27 0.17790609158934245 1103 28-29 0.15393225129238886 1103 30-31 0.05807285491577119 1103 32-33 0.08810690943519006 1103 34-35 0.22325371821090556 1103 36-37 0.18771881596364182 1103 38-39 0.1833805528903909 1103 40-41 0.3120954279888366 1103 42-43 0.41563834021855683 1103 44-45 0.38082505458515215 1103 46-47 0.43316057297713684 1103 48-49 0.3638833425772816 1103 50-51 0.3806620715571185 1103 52-53 0.28312105337155913 1103 54-55 0.36646071538557123 1103 56-57 0.35361232709243495 1103 58-59 0.3975484575632393 1103 60-61 0.4181677683196625 1103 62-63 0.3890471694081583 1103 64-65 0.4859907087376598 1103 66-67 0.418172640643661 1103 68-69 0.4308440471978159 1103 70-71 0.39325967593086375 1103 72-73 0.4622224916006985 1103 74-75 0.4454630126614809 1103 76-77 0.4132735115628954 1103 78-79 0.46073028529370674 1103 80-81 0.8458912712514284 1103 82-83 0.6832014234649506 1103 84-85 0.7571304031572303 1103 86-87 0.790778126144648 1103 88-89 0.8897341859713883 1103 90-91 1.0254404450889574 1103 92-93 1.1601317845438572 1103 94-95 1.099966630136059 1103 96-97 1.197454804136811 1103 98-99 1.5068304509246175 1103 100-101 2.202872299463703 1104 1 0.009109958721197131 1104 2 0.14916895506343764 1104 3 0.15143216305132512 1104 4 -0.01002406218255203 1104 5 0.015029476489708316 1104 6 -0.0028152568232044928 1104 7 -0.06455798368476451 1104 8 0.03217816561809883 1104 9 0.08407563178496957 1104 10-11 0.04725627037270641 1104 12-13 0.04316827510385224 1104 14-15 0.05785183746628064 1104 16-17 0.17503263391645163 1104 18-19 0.05865980824295747 1104 20-21 0.006694729234006047 1104 22-23 -2.775325255370831E-4 1104 24-25 -0.09708894617590857 1104 26-27 -0.031694045383893865 1104 28-29 -0.031735689134471556 1104 30-31 0.04100252324973752 1104 32-33 0.13095725595702135 1104 34-35 0.058502518843297935 1104 36-37 0.17192749740888047 1104 38-39 0.26826881919088663 1104 40-41 0.23876534294277008 1104 42-43 0.2183289527819241 1104 44-45 0.20030617476724188 1104 46-47 0.40876583622470264 1104 48-49 0.6346067196829921 1104 50-51 0.7413410005082142 1104 52-53 0.5058961327615137 1104 54-55 0.5950328801759568 1104 56-57 0.5923499415775311 1104 58-59 0.5833465140425531 1104 60-61 0.6328852354733456 1104 62-63 0.6538099109853235 1104 64-65 0.5951755391225504 1104 66-67 0.713767349797024 1104 68-69 0.7171466387858842 1104 70-71 0.6872676507032551 1104 72-73 0.8444804544475559 1104 74-75 0.6994934159488402 1104 76-77 0.5662397316468493 1104 78-79 0.5038072168888661 1104 80-81 0.6592976115447335 1104 82-83 0.5513506322912036 1104 84-85 0.6672097760787814 1104 86-87 0.9095764245418572 1104 88-89 0.8880247889943398 1104 90-91 0.9450065772647207 1104 92-93 1.096938187190645 1104 94-95 1.101867474463596 1104 96-97 1.2161308232585455 1104 98-99 1.3416514231619843 1104 100-101 1.9148309229849367 1105 1 0.03385736742159651 1105 2 0.01484496727651674 1105 3 0.06210268847534195 1105 4 0.037028707650911485 1105 5 0.026915624195204657 1105 6 0.012732540785350466 1105 7 -0.05967957434187099 1105 8 0.04077223988591072 1105 9 0.08455608118995173 1105 10-11 0.08054800266288709 1105 12-13 0.05506010860111843 1105 14-15 0.03386063907194625 1105 16-17 -0.013275103877568029 1105 18-19 0.125988111545837 1105 20-21 0.14310255970644192 1105 22-23 0.09650885796327202 1105 24-25 0.012453518159958321 1105 26-27 -0.0300949164471902 1105 28-29 -0.04209235885252127 1105 30-31 0.02736515167756437 1105 32-33 0.08959738513821236 1105 34-35 0.12964598842715702 1105 36-37 0.223311419126901 1105 38-39 0.2361639959337154 1105 40-41 0.2671701372023989 1105 42-43 0.14490690214426394 1105 44-45 0.18827077502765377 1105 46-47 0.1578944886351934 1105 48-49 0.39127046764321705 1105 50-51 0.2965073952045145 1105 52-53 0.3557357934334604 1105 54-55 0.3681058231986327 1105 56-57 0.5246222114024661 1105 58-59 0.3743922448657955 1105 60-61 0.5808502905970983 1105 62-63 0.6033229229198014 1105 64-65 0.5094920785222428 1105 66-67 0.5560107926576592 1105 68-69 0.697084322508033 1105 70-71 0.5296774029733129 1105 72-73 0.3476119042676018 1105 74-75 0.506793404008679 1105 76-77 0.6441151786922283 1105 78-79 0.7190471288728091 1105 80-81 0.5920204803678288 1105 82-83 0.5941148936784231 1105 84-85 0.6065935499662096 1105 86-87 0.8717886524056446 1105 88-89 1.0291404550564174 1105 90-91 1.1809029707669971 1105 92-93 1.0520175892727828 1105 94-95 1.1809355163337685 1105 96-97 1.4706382346881242 1105 98-99 1.4917549045722858 1105 100-101 1.8582700878558427 1106 1 -0.1048036976727218 1106 2 0.031140697026870612 1106 3 -0.024066915490962515 1106 4 0.05015298553085046 1106 5 -0.02715207020991528 1106 6 0.029410463032348844 1106 7 -0.06742400124750247 1106 8 -0.11237484749536719 1106 9 -0.020867571621046466 1106 10-11 -0.24041119879663597 1106 12-13 -0.40508406227395 1106 14-15 -0.1585571228666538 1106 16-17 -0.10524031464170491 1106 18-19 -0.044711013683119916 1106 20-21 0.04456526531289029 1106 22-23 -0.027019780030144602 1106 24-25 -0.018723761298467423 1106 26-27 0.039152781357195465 1106 28-29 -0.002492944504318473 1106 30-31 0.009548901512566488 1106 32-33 0.07127666799953403 1106 34-35 -0.26892145645665977 1106 36-37 0.13767532514777514 1106 38-39 0.13361526243512145 1106 40-41 0.05542566238145241 1106 42-43 0.09335114588679261 1106 44-45 0.112113439191873 1106 46-47 0.25987784393724667 1106 48-49 0.30754160688875487 1106 50-51 0.28821239412867783 1106 52-53 0.3418662288842924 1106 54-55 0.24648328835154132 1106 56-57 0.33603112075932273 1106 58-59 0.24886269015282636 1106 60-61 0.27794070048103947 1106 62-63 0.25804737365651675 1106 64-65 0.14330861075479362 1106 66-67 0.3014904792733688 1106 68-69 0.38148813385546276 1106 70-71 0.37341853185829876 1106 72-73 0.4582132370368015 1106 74-75 0.45100765149402733 1106 76-77 0.3259995933749309 1106 78-79 0.4236794556227572 1106 80-81 0.4922598761646597 1106 82-83 0.49926696606129894 1106 84-85 0.6424995292559466 1106 86-87 0.6693507117881019 1106 88-89 0.71679832700136 1106 90-91 0.7787207442775994 1106 92-93 1.02848754998222 1106 94-95 0.7049309218872395 1106 96-97 0.9938482357910807 1106 98-99 1.2244720513866447 1106 100-101 1.4520685117129695 1107 1 -0.04635201903052888 1107 2 0.0135797195829781 1107 3 -0.007568601841107636 1107 4 -0.01326280412093439 1107 5 -0.047910438981041636 1107 6 -0.016032161582728577 1107 7 0.046278204731613926 1107 8 -0.04106733393124529 1107 9 -6.332497894874223E-4 1107 10-11 -0.12295935471540531 1107 12-13 -0.023452401002856504 1107 14-15 -0.041359077303241065 1107 16-17 -0.1432078679111939 1107 18-19 -0.03327621546233672 1107 20-21 -4.983065158441491E-4 1107 22-23 -0.135077318974119 1107 24-25 -0.18677644987076292 1107 26-27 -0.12210915187311144 1107 28-29 -0.06841687450856426 1107 30-31 -0.060224306011896545 1107 32-33 -0.09257119012897874 1107 34-35 -0.11396594639683855 1107 36-37 -0.11818342563712037 1107 38-39 -0.15423079654389227 1107 40-41 -0.1886676471136255 1107 42-43 -0.0626498730672509 1107 44-45 -0.25670750689380384 1107 46-47 -0.42034095858494425 1107 48-49 -0.36304107690836673 1107 50-51 -0.3067867410763796 1107 52-53 -0.3465455657908265 1107 54-55 -0.36457447807110555 1107 56-57 -0.5551212749106824 1107 58-59 -0.32229113430883416 1107 60-61 -0.3035713934883404 1107 62-63 -0.38896494779152135 1107 64-65 -0.18692720566804155 1107 66-67 -0.2941191823662095 1107 68-69 -0.28164485844785503 1107 70-71 -0.03979694496332087 1107 72-73 -0.11236178580506362 1107 74-75 -0.2184975635955162 1107 76-77 -0.1592934875134766 1107 78-79 -0.10369882448808809 1107 80-81 -0.07116625342674254 1107 82-83 -0.07191505823568178 1107 84-85 -0.003275289419114813 1107 86-87 0.17414985376111147 1107 88-89 0.24182051087180767 1107 90-91 0.3579143417869908 1107 92-93 0.28720881905000795 1107 94-95 0.4936281458954568 1107 96-97 0.6240929351620359 1107 98-99 0.70997146107009 1107 100-101 1.1254032644380167 1108 1 -0.012195608797092916 1108 2 0.03538361000894952 1108 3 0.014389258872604671 1108 4 -0.07858982710896356 1108 5 -0.0021173646730474616 1108 6 -0.030294337378599323 1108 7 -0.068057584423876 1108 8 -0.0022328677884360104 1108 9 -0.040736261335140966 1108 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-0.1524553809712259 2316 66-67 -0.23370920105006832 2316 68-69 -0.148597602928497 2316 70-71 -0.17745646520350533 2316 72-73 -0.09206089736838408 2316 74-75 0.07286230443340713 2316 76-77 0.13192389349481104 2316 78-79 0.029413653963260344 2316 80-81 0.14345983855565336 2316 82-83 0.20741519256154106 2316 84-85 0.44303369468856246 2316 86-87 0.3000567033730839 2316 88-89 0.19368347849514223 2316 90-91 0.571771304886866 2316 92-93 0.7245832649059452 2316 94-95 0.8146463862409554 2316 96-97 1.2237450460771448 2316 98-99 1.4611193846729655 2316 100-101 1.8362077943211546 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 8.0 5 30.0 6 71.0 7 161.0 8 388.0 9 791.0 10 1250.0 11 2164.0 12 3506.0 13 5015.0 14 6543.0 15 9571.0 16 12963.0 17 18468.0 18 16402.0 19 10136.0 20 8923.0 21 9179.0 22 9481.0 23 10670.0 24 12383.0 25 14867.0 26 17844.0 27 22569.0 28 27706.0 29 35045.0 30 43467.0 31 54860.0 32 69963.0 33 93084.0 34 127569.0 35 182650.0 36 271868.0 37 382845.0 38 264590.0 39 19029.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.46650767845105 20.170279777718612 15.61328476828695 21.74992777554338 2 15.602370241185914 20.88626409560237 40.0603601792686 23.451005483943117 3 19.031122863541263 25.251335596368197 30.47668120935526 25.240860330735277 4 12.856718184211793 17.54080399079309 37.45003722966029 32.152440595334824 5 13.429714745823846 38.05334989770894 34.173650751767084 14.343284604700123 6 31.391992933442424 37.25695260366974 17.220996962172965 14.130057500714866 7 28.204624405104 33.45375170223067 20.62925203772228 17.71237185494305 8 26.470033662407673 35.48193299793609 19.473122450192943 18.574910889463297 9 26.440986033922876 16.875992672976363 20.459382865296575 36.22363842780418 10-11 24.765056778770884 27.446583223154303 27.193676336941163 20.59468366113365 12-13 25.769918239497652 24.71040383068495 28.02952291131603 21.490155018501365 14-15 22.558795967305848 25.929793071036457 26.653775483914803 24.857635477742893 16-17 21.757296588743902 29.425191032040154 27.104353463773982 21.71315891544196 18-19 21.628677313688907 27.393046122311464 30.093456356362875 20.88482020763675 20-21 22.298672895211805 26.483349258590792 30.453763669179317 20.764214177018083 22-23 22.03793153140298 26.532168813972195 30.229373395607883 21.200526259016943 24-25 21.988375286300336 26.704651301056302 30.297667413147305 21.009305999496057 26-27 21.84694440905423 26.953015953907787 30.113928172024746 21.086111465013236 28-29 21.444032241487825 27.012481133055736 30.409875958777693 21.133610666678745 30-31 21.623019602957996 26.76851296105455 30.363322876912452 21.245144559075 32-33 21.49854959709772 26.899325674481233 30.251078530130048 21.351046198290998 34-35 22.06753432065071 26.84966295125038 29.987259812068075 21.095542916030837 36-37 22.084470867287123 26.71686113389403 29.821475942696317 21.377192056122528 38-39 21.745899355855734 27.005250092861864 30.054828137598637 21.194022413683765 40-41 21.925042396514286 26.865036111354907 30.01047526563292 21.19944622649789 42-43 21.851734821058972 26.904945136153056 29.71821438946451 21.525105653323457 44-45 21.89763683669627 27.165025069858693 29.439601600167602 21.49773649327743 46-47 22.11246471675731 27.43225758961307 28.918669471395447 21.53660822223418 48-49 22.308493741736573 27.507000025480377 28.653588627825137 21.530917604957914 50-51 22.234232601857805 27.621576782281515 28.485588016296116 21.65860259956457 52-53 22.465515840446372 27.780865308147014 28.267229198027593 21.486389653379025 54-55 22.244509686789556 27.713023020104018 28.323079841342192 21.719387451764234 56-57 22.209091964338796 27.983454742605733 28.266598341510647 21.54085495154482 58-59 22.417408192790187 27.527299391585245 28.350145662815358 21.705146752809213 60-61 22.318912384312014 27.53400922389606 28.758284661096845 21.388793730695077 62-63 22.26693241754975 27.63845045340913 28.687477527724063 21.407139601317052 64-65 22.079764901065364 27.590660592900036 28.532018923425806 21.797555582608794 66-67 22.08525733764046 27.88181635443769 28.28160345174158 21.751322856180266 68-69 22.108699283435207 27.686070444745802 28.46947875644441 21.735751515374577 70-71 22.05131181468406 27.718940129610182 28.440261258787192 21.789486796918574 72-73 22.281434228478066 27.656889088705423 28.205963032494 21.85571365032251 74-75 22.163708583772397 27.9118548864283 28.033056540954043 21.89137998884526 76-77 22.15479045222005 27.757358874107123 28.235795398244118 21.852055275428707 78-79 22.21716075002902 27.833092213480253 28.109809095361722 21.839937941129005 80-81 22.124185689654684 28.1979995073794 28.020995829711815 21.6568189732541 82-83 22.185542866574693 28.081337194669135 27.890091789301074 21.843028149455098 84-85 22.136293513794946 27.90522537836267 27.836569862861673 22.121911244980716 86-87 22.17961966292294 27.74303324056589 28.11532984346556 21.962017253045612 88-89 22.222539940489167 27.67774685529695 28.007547853561448 22.09216535065244 90-91 22.142171737406347 27.772616731586368 28.06003399400702 22.025177537000264 92-93 22.142687675640232 27.534046530116267 28.171065304899834 22.152200489343667 94-95 21.984581541449494 27.532027416884432 28.15202158470951 22.33136945695657 96-97 22.14595725525391 27.447432569016428 28.054290187507252 22.352319988222405 98-99 21.9284258662093 27.53152927680137 27.86492734945701 22.675117507532317 100-101 21.974316153573024 27.457461164748903 28.296232367626594 22.271990314051475 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 547.0 1 395.0 2 281.5 3 358.5 4 329.0 5 297.5 6 563.0 7 1333.0 8 2277.5 9 2719.0 10 2486.5 11 2182.0 12 2214.0 13 2427.0 14 2664.0 15 2850.5 16 2962.5 17 3103.5 18 3290.0 19 3513.0 20 3823.0 21 4272.5 22 5069.5 23 6073.5 24 7288.5 25 8965.5 26 11182.0 27 13662.5 28 16122.0 29 19025.5 30 22303.5 31 25587.5 32 29300.0 33 33329.5 34 37976.5 35 41468.0 36 45462.0 37 50312.0 38 52949.5 39 54021.0 40 55219.5 41 57936.0 42 60567.0 43 62936.5 44 65104.5 45 66764.5 46 66334.0 47 66437.5 48 70006.0 49 69637.0 50 65873.5 51 62032.0 52 57613.0 53 55115.0 54 52902.5 55 48398.5 56 42835.5 57 37663.0 58 33834.5 59 30343.0 60 25922.5 61 20928.0 62 16398.5 63 12791.5 64 9840.0 65 7644.0 66 5599.5 67 4370.5 68 3140.0 69 1901.0 70 1390.0 71 1040.0 72 804.0 73 645.0 74 482.0 75 359.0 76 237.5 77 135.0 78 77.0 79 42.0 80 24.0 81 12.5 82 5.5 83 2.0 84 2.5 85 1.0 86 0.0 87 0.5 88 1.0 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04161794724429735 2 0.0 3 0.0 4 0.0 5 0.0014721994943560966 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006483340080914349 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.4155764368808623E-4 22-23 8.210343333909002E-4 24-25 0.0 26-27 0.0022649222990093797 28-29 0.004501533069281142 30-31 2.8311528737617246E-4 32-33 2.2649222990093795E-4 34-35 0.0 36-37 2.8311528737617244E-5 38-39 5.662305747523449E-5 40-41 0.0 42-43 0.008380212506334705 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.8311528737617244E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 2.8311528737617244E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.379190460147277E-4 84-85 8.493458621285174E-5 86-87 0.0 88-89 0.0 90-91 0.009371116012151308 92-93 0.0014721994943560966 94-95 0.0 96-97 0.0 98-99 4.529844598018759E-4 100-101 0.011777595954848773 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1766065.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.610339791820515 #Duplication Level Percentage of deduplicated Percentage of total 1 76.00060046324425 41.5041861568016 2 13.600897710218932 14.854992908576989 3 4.457198877706672 7.3022743569384705 4 1.9261784029279787 4.207570283342523 5 1.07812180918563 2.943829916829976 6 0.6452981765838566 2.114397161417196 7 0.41992449489719685 1.6052553547271162 8 0.2964389956325076 1.295090742322979 9 0.22099966336281887 1.0861980039109336 >10 1.198447430393504 12.047849350524215 >50 0.10073911204158863 3.779156319440929 >100 0.05255842755607575 5.094939016803683 >500 0.0019732915492506817 0.6715281708593823 >1k 3.115723498816866E-4 0.22981690441910618 >5k 3.115723498816866E-4 1.262915353084955 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9419 0.5333325783592336 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7539 0.4268812303057928 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5309 0.3006118121360199 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1875 0.10616823276606467 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.662305747523449E-5 0.0 5 0.0 0.0 0.0 5.662305747523449E-5 0.0 6 0.0 0.0 0.0 5.662305747523449E-5 0.0 7 0.0 0.0 0.0 5.662305747523449E-5 0.0 8 0.0 0.0 0.0 5.662305747523449E-5 0.0 9 0.0 0.0 0.0 5.662305747523449E-5 0.0 10-11 0.0 0.0 0.0 5.662305747523449E-5 0.0 12-13 0.0 0.0 0.0 5.662305747523449E-5 0.0 14-15 0.0 0.0 0.0 5.662305747523449E-5 0.0 16-17 0.0 0.0 0.0 5.662305747523449E-5 0.0 18-19 0.0 0.0 0.0 5.662305747523449E-5 0.0 20-21 0.0 0.0 0.0 5.662305747523449E-5 0.0 22-23 0.0 0.0 0.0 1.4155764368808623E-4 0.0 24-25 0.0 0.0 0.0 2.8311528737617246E-4 0.0 26-27 0.0 0.0 0.0 3.680498735890242E-4 0.0 28-29 0.0 0.0 0.0 9.625919770789864E-4 0.0 30-31 0.0 0.0 0.0 0.0022366107702717626 0.0 32-33 0.0 0.0 0.0 0.0034540065059893038 0.0 34-35 0.0 0.0 0.0 0.005464125046360128 0.0 36-37 0.0 0.0 0.0 0.008210343333909001 0.0 38-39 0.0 0.0 0.0 0.013617845322793896 0.0 40-41 0.0 0.0 0.0 0.019365085656530195 0.0 42-43 0.0 0.0 0.0 0.024121422484449893 0.0 44-45 0.0 0.0 0.0 0.028226594151404397 0.0 46-47 0.0 0.0 0.0 0.03391721142766546 0.0 48-49 0.0 0.0 0.0 0.03935302494528797 0.0 50-51 0.0 0.0 0.0 0.04490208457786095 0.0 52-53 0.0 0.0 0.0 0.05837837225696676 0.0 54-55 0.0 0.0 0.0 0.08136733359191196 0.0 56-57 0.0 0.0 0.0 0.11579415253685453 0.0 58-59 0.0 0.0 0.0 0.1621684366090716 0.0 60-61 0.0 0.0 0.0 0.21528086452084153 0.0 62-63 0.0 0.0 0.0 0.24916976441976937 0.0 64-65 0.0 0.0 0.0 0.29067446554911625 0.0 66-67 0.0 0.0 0.0 0.36728546231310855 0.0 68-69 0.0 0.0 0.0 0.4716134457112281 0.0 70-71 0.0 0.0 0.0 0.5890213553861268 0.0 72-73 0.0 0.0 0.0 0.7319379524536187 0.0 74-75 0.0 0.0 0.0 0.87474130340616 0.0 76-77 0.0 0.0 0.0 1.0051725163003629 0.0 78-79 0.0 0.0 0.0 1.138208389838426 0.0 80-81 0.0 0.0 0.0 1.2844657472969567 0.0 82-83 0.0 0.0 0.0 1.4522681781248141 0.0 84-85 0.0 0.0 0.0 1.7046088337631966 0.0 86-87 0.0 0.0 0.0 2.0269638999697066 0.0 88-89 0.0 0.0 0.0 2.3982129763060813 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5580 0.0 45.814777 1 GTATCAA 9085 0.0 35.671135 1 ATCAACG 11525 0.0 27.778212 3 TCAACGC 11655 0.0 27.468374 4 TATCAAC 11675 0.0 27.462006 2 CAACGCA 11835 0.0 27.050606 5 AACGCAG 12100 0.0 26.417421 6 TAGGCCG 90 8.9504535E-4 26.388386 5 TACCGTC 140 1.778496E-5 23.752909 7 GTACATG 7700 0.0 23.573832 1 ACGCAGA 13940 0.0 22.935019 7 CGCAGAG 14015 0.0 22.778387 8 GAGTACT 8410 0.0 22.453701 12-13 TACATGG 8070 0.0 22.130928 2 ACATGGG 7795 0.0 21.936722 3 GCAGAGT 14580 0.0 21.406942 9 GTACTTT 8920 0.0 21.059225 14-15 AGTACTT 8790 0.0 20.915533 12-13 AGAGTAC 12970 0.0 20.56632 10-11 CATGGGG 3185 0.0 20.282188 4 >>END_MODULE