FastQCFastQC Report
Thu 26 May 2016
SRR937378_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937378_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1710364
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80610.47130318458526954No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59540.3481130332490628No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42130.24632183558587528No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27050.15815346908611266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47750.043.129311
GTATCAA81750.039.7366261
TATCAAC103450.031.2965342
ATCAACG103150.031.1571033
TCAACGC104100.030.785044
CAACGCA105900.030.1728695
AACGCAG109000.029.2385276
ACGCAGA124100.025.6808977
CGCAGAG123600.025.5933978
GTACATG78950.025.542991
TACATGG79750.024.2629362
GCAGAGT128200.023.9711259
ACATGGG78650.023.5142153
GAGTACT71100.022.61282712-13
AGTACTT73750.021.76809712-13
TAGCGCG1100.002861176621.6126444
GTACTTT75350.021.17855614-15
AGAGTAC115700.020.93673710-11
CATGGGG33250.020.1631344
ATTCGCG1552.6011548E-1019.91282880-81
Produced by FastQC (version 0.11.5)