##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937378_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1710364 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.576425252168544 33.0 31.0 34.0 30.0 34.0 2 31.698551302529754 33.0 31.0 34.0 30.0 34.0 3 31.72625943951112 33.0 31.0 34.0 30.0 34.0 4 35.35202506600934 37.0 35.0 37.0 33.0 37.0 5 35.345194940959935 37.0 35.0 37.0 33.0 37.0 6 35.38467074844887 37.0 35.0 37.0 33.0 37.0 7 35.324234490435956 37.0 35.0 37.0 33.0 37.0 8 35.31409805164281 37.0 35.0 37.0 33.0 37.0 9 36.914377290448115 39.0 37.0 39.0 33.0 39.0 10-11 36.78332623932683 39.0 37.0 39.0 33.0 39.0 12-13 36.64507292015033 39.0 37.0 39.0 32.0 39.0 14-15 37.792638584535226 40.0 38.0 41.0 32.5 41.0 16-17 37.75227027697028 40.0 38.0 41.0 32.5 41.0 18-19 37.72597528947054 40.0 37.5 41.0 32.0 41.0 20-21 37.66539637176648 40.0 37.5 41.0 32.0 41.0 22-23 37.51475095359818 40.0 37.0 41.0 32.0 41.0 24-25 37.34136242343735 40.0 37.0 41.0 32.0 41.0 26-27 37.18209135599206 39.5 37.0 41.0 31.0 41.0 28-29 37.2305193514363 40.0 37.0 41.0 31.0 41.0 30-31 37.0692174297401 39.0 36.5 41.0 31.0 41.0 32-33 36.873367014272986 39.0 36.0 41.0 31.0 41.0 34-35 36.74076570835214 39.0 36.0 41.0 30.0 41.0 36-37 36.73002384287789 39.0 36.0 41.0 30.0 41.0 38-39 36.643329723965195 39.0 36.0 41.0 30.0 41.0 40-41 36.45564511413944 39.0 35.5 41.0 30.0 41.0 42-43 36.210739351389535 39.0 35.0 41.0 30.0 41.0 44-45 35.97391315532834 39.0 35.0 41.0 28.5 41.0 46-47 35.61386698971681 39.0 35.0 41.0 27.0 41.0 48-49 35.28082004766237 39.0 35.0 40.0 26.0 41.0 50-51 34.41050559997755 38.0 34.0 39.5 24.5 40.5 52-53 34.50483259703782 38.0 34.0 39.5 24.0 40.5 54-55 34.81136530001801 38.0 34.0 40.0 24.5 41.0 56-57 34.61119592086831 38.0 34.0 40.0 23.5 41.0 58-59 34.35606864971433 38.0 34.0 40.0 23.0 41.0 60-61 34.070848076783655 38.0 34.0 40.0 22.5 41.0 62-63 33.74015297328522 37.5 33.0 40.0 22.0 41.0 64-65 33.364207268160456 37.0 33.0 40.0 20.5 41.0 66-67 32.99126647894834 36.0 32.5 39.0 19.5 41.0 68-69 32.51068164437511 36.0 32.0 39.0 18.0 40.5 70-71 32.06239519774738 35.0 31.0 39.0 14.0 40.0 72-73 31.67016377800281 35.0 31.0 38.0 11.5 40.0 74-75 31.207007104920358 35.0 31.0 37.0 7.5 39.0 76-77 30.75923546098959 35.0 30.5 37.0 7.0 39.0 78-79 30.282048733485972 34.5 30.0 36.0 2.0 39.0 80-81 29.78193326099006 34.0 29.5 36.0 2.0 37.5 82-83 29.2945770607894 34.0 29.0 35.0 2.0 37.0 84-85 28.846780568346855 34.0 29.0 35.0 2.0 36.5 86-87 28.397780823263354 34.0 28.5 35.0 2.0 36.0 88-89 27.97578322509127 34.0 27.0 35.0 2.0 36.0 90-91 27.539676057260326 33.5 26.5 35.0 2.0 35.0 92-93 27.07020873919236 33.0 25.5 35.0 2.0 35.0 94-95 26.512377189884724 33.0 25.0 35.0 2.0 35.0 96-97 25.851028494519298 32.0 23.5 35.0 2.0 35.0 98-99 25.049825358812512 32.0 19.0 34.0 2.0 35.0 100-101 23.115693501500267 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05127357618151507 1101 2 0.0706629799108427 1101 3 0.1025446738687954 1101 4 0.037243346526729226 1101 5 0.082319663820563 1101 6 -0.03718957810086465 1101 7 0.04998575645831238 1101 8 0.1558021277995465 1101 9 0.22650033646145573 1101 10-11 0.13085017359001228 1101 12-13 0.018352764886294892 1101 14-15 0.07829017646414371 1101 16-17 0.25051953942742955 1101 18-19 0.06922647456593012 1101 20-21 0.15403516538122375 1101 22-23 0.11098868457676758 1101 24-25 0.2555460771924487 1101 26-27 0.03367193524198342 1101 28-29 0.016702954464676623 1101 30-31 0.06001798577695183 1101 32-33 0.04306789998012306 1101 34-35 0.04106013715434642 1101 36-37 0.003652827808600989 1101 38-39 0.01050100478975935 1101 40-41 -0.1353949552236955 1101 42-43 0.1179339397708361 1101 44-45 -0.0026239512448000823 1101 46-47 -0.029626303096797812 1101 48-49 0.04411449976369397 1101 50-51 -0.1901400761464913 1101 52-53 -0.15133789380700335 1101 54-55 0.015804321463129156 1101 56-57 0.011811344782451272 1101 58-59 0.07473210857467194 1101 60-61 -0.055020692863713805 1101 62-63 0.04941546690704968 1101 64-65 0.10983774209056918 1101 66-67 0.18074665936514123 1101 68-69 0.3245912387353016 1101 70-71 0.3354128489586401 1101 72-73 0.2868997660610617 1101 74-75 0.22787601016968395 1101 76-77 0.3185574173731247 1101 78-79 0.30404285114611795 1101 80-81 0.18101331374510465 1101 82-83 0.23099589065038373 1101 84-85 0.2790121650290409 1101 86-87 0.4183107963709709 1101 88-89 0.37938488050314945 1101 90-91 0.45921001702711806 1101 92-93 0.5939135408005001 1101 94-95 0.7058278904576163 1101 96-97 0.7898419148125058 1101 98-99 0.9492959085275352 1101 100-101 1.3454838296080887 1102 1 0.02481746656177819 1102 2 -0.03009869020933209 1102 3 -0.0367431681291599 1102 4 -0.10063045691063621 1102 5 0.014119450676354006 1102 6 -0.0477761756499433 1102 7 0.13977334804742014 1102 8 -0.00535198400859116 1102 9 0.18969913734726163 1102 10-11 0.03786037508012896 1102 12-13 8.010038356758287E-4 1102 14-15 0.11546550537794786 1102 16-17 0.17836707960558584 1102 18-19 0.2732168166172855 1102 20-21 0.17069922115000224 1102 22-23 0.05470009965969069 1102 24-25 0.1628004569736632 1102 26-27 0.1713614923624931 1102 28-29 0.1494617715855142 1102 30-31 0.0942667355128961 1102 32-33 0.05213157265897195 1102 34-35 0.1228438432526886 1102 36-37 0.1482514734646614 1102 38-39 0.15409742168844076 1102 40-41 0.1980472644192801 1102 42-43 0.3211801759241695 1102 44-45 0.1367045376861853 1102 46-47 0.14385480705698228 1102 48-49 0.04878771028988638 1102 50-51 -0.09386737023565672 1102 52-53 -0.11440199609723578 1102 54-55 -0.041834513032050324 1102 56-57 -0.07071498374920537 1102 58-59 -0.04092236045177344 1102 60-61 -0.12151788852202827 1102 62-63 -0.22745927645355835 1102 64-65 -0.15713428095178728 1102 66-67 -0.15276646989092058 1102 68-69 -0.1510956647635382 1102 70-71 -0.02929487289053867 1102 72-73 -0.07314067912076183 1102 74-75 0.03865385598904325 1102 76-77 0.09283995450127946 1102 78-79 0.001978580334299096 1102 80-81 0.07492125823266704 1102 82-83 0.06479562528821248 1102 84-85 0.023672285462637888 1102 86-87 -0.03356471839128261 1102 88-89 -0.04003984375043146 1102 90-91 0.03658931640453744 1102 92-93 0.0849400627759529 1102 94-95 0.0338791993481955 1102 96-97 0.1257974203654122 1102 98-99 0.1752673405575571 1102 100-101 0.620367466636317 1103 1 -0.006356698893114299 1103 2 -0.05857892842749379 1103 3 -0.04941824379595161 1103 4 -0.14389315211675324 1103 5 -0.0820732900482497 1103 6 -0.043302085506603305 1103 7 -0.12846207237890184 1103 8 -0.09283732036070802 1103 9 -0.05861385239489181 1103 10-11 0.08429203656024242 1103 12-13 -0.036517289292028465 1103 14-15 -0.07157731642104181 1103 16-17 -0.14830682612709012 1103 18-19 -0.14934313658534393 1103 20-21 -0.35221277801635864 1103 22-23 -0.3574170863724717 1103 24-25 -0.2787711229426293 1103 26-27 -0.1692055783340578 1103 28-29 -0.21418248634311965 1103 30-31 -0.2649387592302972 1103 32-33 -0.272757038877117 1103 34-35 -0.28861278787387334 1103 36-37 -0.17066056675201224 1103 38-39 -0.2993676989600331 1103 40-41 -0.17127831829790097 1103 42-43 -0.12893756744501417 1103 44-45 0.02238512041125773 1103 46-47 -0.02726226687622102 1103 48-49 -0.11548757789125119 1103 50-51 -0.05942852021300382 1103 52-53 -0.03180835794949388 1103 54-55 -0.11926074481794302 1103 56-57 -0.151585692609288 1103 58-59 -0.09950550866395247 1103 60-61 -0.11851845217449153 1103 62-63 -0.18783802091374469 1103 64-65 0.09603133024800314 1103 66-67 -0.0549084282122152 1103 68-69 0.010852780771365644 1103 70-71 0.02862811513066532 1103 72-73 0.11994542076729431 1103 74-75 0.1843061139934612 1103 76-77 0.2284358709417731 1103 78-79 0.2313709250811975 1103 80-81 0.15736174711040007 1103 82-83 0.15072657549259816 1103 84-85 0.15643138797633682 1103 86-87 0.2962530892374424 1103 88-89 0.3763805209974471 1103 90-91 0.4089346632718467 1103 92-93 0.3597674146040326 1103 94-95 0.23578381577243235 1103 96-97 0.33482170162957203 1103 98-99 0.5107613416848586 1103 100-101 0.8535443350968812 1104 1 0.05288342426533532 1104 2 0.0074251706835859466 1104 3 -0.015594540985929939 1104 4 -0.07185950447530587 1104 5 -0.01723843963602434 1104 6 -0.012772843021203073 1104 7 0.05095170564050733 1104 8 -0.004920321524920723 1104 9 0.05257644720722965 1104 10-11 0.11932543188058986 1104 12-13 0.21727346287836014 1104 14-15 0.21721262486098425 1104 16-17 0.17533521756912762 1104 18-19 0.2593281976861732 1104 20-21 0.2500014099946455 1104 22-23 0.08003293735054484 1104 24-25 0.025685865566899224 1104 26-27 -0.012255721819038001 1104 28-29 -0.07919482673594302 1104 30-31 -0.034110031327010404 1104 32-33 0.01429925126235787 1104 34-35 0.04245733525640816 1104 36-37 0.012029159996714611 1104 38-39 0.14117285571980176 1104 40-41 0.062404314083138956 1104 42-43 0.18514565183409104 1104 44-45 0.3363614955913974 1104 46-47 0.2760006231298888 1104 48-49 0.30052863424197085 1104 50-51 0.3073039900518779 1104 52-53 0.3028483867703784 1104 54-55 0.4634917201418247 1104 56-57 0.4155756830276047 1104 58-59 0.4607188478468416 1104 60-61 0.44349388298679315 1104 62-63 0.23122878139731284 1104 64-65 0.17440781504897274 1104 66-67 0.26790502038128494 1104 68-69 0.31881339051112434 1104 70-71 0.35671555746804984 1104 72-73 0.3810034831908098 1104 74-75 0.30832152851838046 1104 76-77 0.46142079727166063 1104 78-79 0.38603711473892943 1104 80-81 0.39355964250298214 1104 82-83 0.4946852962447821 1104 84-85 0.582959872291184 1104 86-87 0.5439380331533492 1104 88-89 0.467913719732703 1104 90-91 0.4410483574659274 1104 92-93 0.7263989159467279 1104 94-95 0.87358755181479 1104 96-97 1.0807690707495752 1104 98-99 1.038253173443433 1104 100-101 1.2355399575070187 1105 1 0.030049647410660185 1105 2 -0.09544365517479036 1105 3 0.018487369833824374 1105 4 -0.07275520123651802 1105 5 0.05997321740298389 1105 6 0.03604263046487688 1105 7 0.09428265910322153 1105 8 0.14949001144061214 1105 9 0.0832183632194301 1105 10-11 0.07431991490778245 1105 12-13 -0.04759964674713757 1105 14-15 0.06402900702493497 1105 16-17 -0.10232428785048597 1105 18-19 0.023328327497296186 1105 20-21 -0.032973860986075465 1105 22-23 -0.05469802909328081 1105 24-25 0.1258708464197582 1105 26-27 0.2181038549966985 1105 28-29 0.24376876124354396 1105 30-31 0.020611848338084826 1105 32-33 0.1086914106704242 1105 34-35 0.1810436921071883 1105 36-37 0.020359788931614275 1105 38-39 0.22079899968034056 1105 40-41 0.20852620672933853 1105 42-43 0.13536957602438804 1105 44-45 0.11635760125765415 1105 46-47 0.13935494259507664 1105 48-49 0.20214802802401266 1105 50-51 0.14311563410977612 1105 52-53 0.13188713539113195 1105 54-55 0.07318536588726232 1105 56-57 0.15857561812204324 1105 58-59 0.14224997006401452 1105 60-61 0.1627091747372944 1105 62-63 0.20458829330748074 1105 64-65 0.28661330605955726 1105 66-67 0.23517639053770978 1105 68-69 0.22094878351430225 1105 70-71 0.25064364210490453 1105 72-73 0.3055248046680603 1105 74-75 0.3662270260195193 1105 76-77 0.4074550674086126 1105 78-79 0.3934655563563787 1105 80-81 0.5432837938627593 1105 82-83 0.7249215524919421 1105 84-85 0.6360493770617417 1105 86-87 0.5615663839030098 1105 88-89 0.5251506899410536 1105 90-91 0.7433395238188822 1105 92-93 0.7795598168359348 1105 94-95 0.7334629331349696 1105 96-97 0.9243726275743924 1105 98-99 1.1451039555554594 1105 100-101 1.4581887535088391 1106 1 -0.010744466591120272 1106 2 -0.17224443937363532 1106 3 -0.23051041052289634 1106 4 -0.183070345513336 1106 5 -0.05827179966686202 1106 6 0.0053360627995573395 1106 7 -0.0695720838350482 1106 8 -0.18062840709171724 1106 9 -0.03326784006383576 1106 10-11 -0.31363091492331563 1106 12-13 -0.3426262937337228 1106 14-15 -0.3603048183105457 1106 16-17 -0.34161873423540356 1106 18-19 -0.13852141279316754 1106 20-21 -0.07976101184295459 1106 22-23 -0.07240221937417601 1106 24-25 0.05251952308421437 1106 26-27 0.020054774965593936 1106 28-29 0.06962028175553314 1106 30-31 -0.05401774164760553 1106 32-33 0.13192893503579484 1106 34-35 0.1905960452670783 1106 36-37 0.1321346021773664 1106 38-39 -0.026205608614588982 1106 40-41 -0.029808272105178446 1106 42-43 -0.013004000080080402 1106 44-45 0.029370119144374485 1106 46-47 0.15141988687864227 1106 48-49 0.2414103628183355 1106 50-51 0.20985873011780143 1106 52-53 -0.0038288404068680393 1106 54-55 0.021642882816273357 1106 56-57 -0.09809514230295235 1106 58-59 -0.09117143540960626 1106 60-61 0.04811980874403332 1106 62-63 0.08226848439961998 1106 64-65 0.23280836425477247 1106 66-67 0.32956925613098775 1106 68-69 0.16206401342202525 1106 70-71 0.2048744850059876 1106 72-73 0.2174567956993485 1106 74-75 0.16955930774520667 1106 76-77 0.40695311254511424 1106 78-79 0.4000709668777098 1106 80-81 0.21341325427536262 1106 82-83 0.15667878886704756 1106 84-85 -0.007806605271248657 1106 86-87 0.15695245535974678 1106 88-89 0.24339852381708837 1106 90-91 0.4560827550336022 1106 92-93 0.5633029015767299 1106 94-95 0.5133858653175416 1106 96-97 0.4203175533797179 1106 98-99 0.535936378121459 1106 100-101 0.5743611645822675 1107 1 -0.016451805908221928 1107 2 -0.06564824273887027 1107 3 -0.06505233622001327 1107 4 0.027284532890341495 1107 5 -0.05856923116939328 1107 6 0.13051829115271119 1107 7 -0.007156839210239241 1107 8 0.024960234599561204 1107 9 0.21560556364786976 1107 10-11 0.04212145158195568 1107 12-13 0.06490321454188575 1107 14-15 0.050988871237876765 1107 16-17 0.08729949043308949 1107 18-19 -0.004148253121954326 1107 20-21 -0.17690814419538725 1107 22-23 -0.03277256513256788 1107 24-25 0.041450511184663696 1107 26-27 0.07262166841042017 1107 28-29 -0.007956865972602145 1107 30-31 0.10121079711129255 1107 32-33 0.0572471615374468 1107 34-35 0.347927456829332 1107 36-37 0.1768822322349024 1107 38-39 0.2648440633629221 1107 40-41 0.2899969308838024 1107 42-43 0.17213897556399615 1107 44-45 0.20464056870589786 1107 46-47 0.29387685898928595 1107 48-49 0.4430739627477607 1107 50-51 0.3409324165956633 1107 52-53 0.23883780028992163 1107 54-55 0.4139536597815976 1107 56-57 0.38984421650071965 1107 58-59 0.4973653070050972 1107 60-61 0.4979760196316789 1107 62-63 0.5824507700392587 1107 64-65 0.5452932692622952 1107 66-67 0.5481312275198107 1107 68-69 0.5817750374067359 1107 70-71 0.5446135564605896 1107 72-73 0.5351159318647731 1107 74-75 0.5211110844286857 1107 76-77 0.38245992620129954 1107 78-79 0.5862445266378451 1107 80-81 0.7884850342585459 1107 82-83 0.896883943277075 1107 84-85 0.9253757972328565 1107 86-87 0.9161224198192031 1107 88-89 0.768586137101142 1107 90-91 0.8512520137233928 1107 92-93 0.885528573719224 1107 94-95 1.001777558316899 1107 96-97 1.0407642505839085 1107 98-99 1.3156141071929817 1107 100-101 1.4986821477102445 1108 1 -0.17163687560692864 1108 2 -0.19639658628490508 1108 3 -0.15039339216359338 1108 4 -0.1934056614145092 1108 5 -0.19966836930984044 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-1.1962031124589814 2316 58-59 -1.178243877624709 2316 60-61 -1.0924644677160273 2316 62-63 -1.2206771587329968 2316 64-65 -0.9372512480760307 2316 66-67 -1.033230065826011 2316 68-69 -1.066010810765711 2316 70-71 -1.3532894426022075 2316 72-73 -1.5078784448924907 2316 74-75 -1.3486757009508814 2316 76-77 -1.36718862112766 2316 78-79 -1.1804494752365748 2316 80-81 -1.515111498075104 2316 82-83 -1.4679825047079262 2316 84-85 -1.4904512880529914 2316 86-87 -1.5967641781189421 2316 88-89 -1.8471003671162904 2316 90-91 -1.9290314914312496 2316 92-93 -1.7886162247812472 2316 94-95 -1.810663866237924 2316 96-97 -1.7365904823120601 2316 98-99 -1.7904660240160162 2316 100-101 -2.555500139075132 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7676.0 3 1638.0 4 1509.0 5 1668.0 6 1853.0 7 2264.0 8 2628.0 9 3472.0 10 4111.0 11 5212.0 12 6564.0 13 8438.0 14 9561.0 15 11466.0 16 14317.0 17 18502.0 18 15974.0 19 11533.0 20 11530.0 21 11609.0 22 12595.0 23 14079.0 24 15822.0 25 18121.0 26 20763.0 27 24373.0 28 28629.0 29 34308.0 30 42061.0 31 51621.0 32 65148.0 33 84752.0 34 114668.0 35 160525.0 36 234279.0 37 329114.0 38 271685.0 39 36289.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.79843578605976 19.323782073516647 15.225907777580186 22.651874362843404 2 14.765798689528548 20.1589107952579 41.09524675461803 23.98004376059552 3 18.536636582208466 25.030867510486154 30.18863055006637 26.24386535723901 4 12.174058926908014 16.764803878091644 37.92401962509591 33.13711756990443 5 13.316443191990343 37.60809553586408 34.40913639717916 14.666324874966415 6 31.075544031550873 36.74903894450641 17.788449985552663 14.386967038390058 7 27.939308133347712 33.11213415405964 21.01630513766543 17.93225257492722 8 26.143306306755083 34.958170827118785 19.93500728164586 18.963515584480277 9 26.253881616768464 16.7397525024305 20.591277401328263 36.41508847947277 10-11 24.49188808672089 26.98425212080857 27.657670753444613 20.866189039025926 12-13 25.466574185507945 24.43843922513917 28.26020259703269 21.834783992320194 14-15 22.3569692467948 25.570240402717403 26.80883669346273 25.263953657025066 16-17 21.548495381155067 29.12784908862568 27.22003728362808 22.10361824659117 18-19 21.484648318329548 27.209752598657573 30.09836749571334 21.207231587299542 20-21 22.138610473321275 26.33462466853565 30.270184278211204 21.25658057993187 22-23 21.832059860965472 26.3685689498929 30.134226595165874 21.665144593975757 24-25 21.677470271898137 26.594563209073563 30.145383608303568 21.58258291072473 26-27 21.516692642113032 26.84108300033983 29.974219857740486 21.66800449980665 28-29 21.223497476075682 26.872593027129803 30.27569335030112 21.62821614649339 30-31 21.38776907323809 26.654823126436728 30.189217259605634 21.768190540719544 32-33 21.209227253693214 26.818826898298425 30.087796695895115 21.884149152113245 34-35 21.752457193632544 26.831077425703924 29.82955994122312 21.58690543944041 36-37 21.732055776180424 26.60108956620968 29.732061635638395 21.9347930219715 38-39 21.44079438278978 26.88884611587327 29.895818410384816 21.77454109095213 40-41 21.658168608696286 26.706820661925722 29.956130393068747 21.678880336309238 42-43 21.53542349690753 26.744835385701688 29.669189277876907 22.050551839513876 44-45 21.575864655485624 26.94868323898597 29.47851106969985 21.996941035828556 46-47 21.7237980319845 27.198663376287584 28.96640215097495 22.11113644075296 48-49 21.882393002294844 27.323027294601825 28.730058582835177 22.06452112026815 50-51 21.80825670570507 27.428866497219893 28.561334778700814 22.20154201837422 52-53 21.98997983891061 27.508601732599114 28.432295028435988 22.069123400054284 54-55 21.844787085400274 27.383889805793377 28.525797316178885 22.24552579262746 56-57 21.81922361364706 27.729783076187125 28.433538282800974 22.01745502736484 58-59 21.97835289485872 27.301868702506532 28.529840565781008 22.189937836853744 60-61 21.892037797052478 27.297500202846177 28.90541861445372 21.90504338564763 62-63 21.823541801974567 27.405477820343922 28.865997502278447 21.904982875403068 64-65 21.6803873184152 27.307231287304116 28.709028446625375 22.303352947655302 66-67 21.66410606036196 27.556800789711488 28.492884248390038 22.28620890153651 68-69 21.700504231709335 27.462922200693885 28.635323166686437 22.20125040091034 70-71 21.62521761520973 27.449000485981667 28.60563100135366 22.320150897454937 72-73 21.79246366350602 27.336532647771534 28.497490075133697 22.37351361358875 74-75 21.62351954752433 27.630112975365613 28.361817648625316 22.384549828484744 76-77 21.67504296552035 27.518822501568906 28.39524294196476 22.41089159094598 78-79 21.775892326127146 27.541038359110182 28.33546716198836 22.347602152774318 80-81 21.64858418775036 27.815914085349224 28.278393515327068 22.257108211573353 82-83 21.721758199138012 27.7291231355143 28.30031434703784 22.24880431830984 84-85 21.7347916123571 27.553682261933705 28.11140369119555 22.600122434513644 86-87 21.7391559056871 27.42930120637208 28.36950106255062 22.4620418253902 88-89 21.837118493630207 27.33992038127935 28.22483647111551 22.59812465397493 90-91 21.654800225454572 27.354441771435468 28.44462711524783 22.54613088786213 92-93 21.735915909652793 27.109669663476915 28.516467614881957 22.637946811988336 94-95 21.613541032010122 27.100869381825 28.440560997328586 22.845028588836293 96-97 21.61051826812386 27.06189749463219 28.377676947276843 22.949907289967104 98-99 21.422481982773775 27.107605320208588 28.25364738969942 23.216265307318213 100-101 21.4251235746011 26.866360175880722 28.786116061124762 22.922400188393418 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2644.0 1 1526.5 2 405.5 3 434.0 4 386.5 5 354.5 6 617.5 7 1305.0 8 2220.0 9 2624.0 10 2371.0 11 2087.0 12 2098.5 13 2241.5 14 2397.5 15 2542.5 16 2648.0 17 2811.5 18 3026.0 19 3246.5 20 3547.5 21 4026.5 22 4713.5 23 5636.5 24 6794.5 25 8449.0 26 10722.0 27 13466.0 28 15703.5 29 18150.5 30 21625.5 31 24892.5 32 28112.0 33 31914.0 34 36622.0 35 40127.5 36 43872.5 37 48393.5 38 50781.5 39 51739.0 40 53194.0 41 55905.0 42 58461.5 43 60679.5 44 62634.5 45 64392.5 46 63871.5 47 63760.5 48 67247.5 49 67247.5 50 63372.0 51 59547.0 52 56040.5 53 53788.5 54 51529.0 55 47369.0 56 42396.5 57 37564.5 58 33446.0 59 29539.5 60 25116.0 61 20515.0 62 16137.5 63 12528.0 64 9717.0 65 7596.0 66 5571.5 67 4257.5 68 3124.0 69 2043.5 70 1541.0 71 1176.0 72 878.0 73 654.5 74 479.5 75 352.0 76 241.0 77 144.5 78 78.5 79 59.0 80 33.5 81 20.5 82 21.0 83 15.5 84 10.5 85 14.0 86 12.5 87 6.0 88 8.0 89 8.0 90 3.5 91 4.5 92 7.0 93 6.0 94 6.5 95 7.0 96 5.0 97 8.0 98 8.0 99 4.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38032839793166834 2 0.32940356555680544 3 0.3213935747010578 4 0.32788342130680953 5 0.3287019605183458 6 0.24363234960511332 7 0.22848937419169252 8 0.23497922079744432 9 0.22936638048976712 10-11 0.25099920250893965 12-13 0.23799027575416692 14-15 0.23132502788880027 16-17 0.23299133985514195 18-19 0.22837244001861595 20-21 0.21822840050422015 22-23 0.22273036616766956 24-25 0.21068614634077892 26-27 0.21188472161481417 28-29 0.23348831009071752 30-31 0.24731577605702645 32-33 0.23927655165800962 34-35 0.24854358487433084 36-37 0.21758526255229882 38-39 0.21518811200422835 40-41 0.21056921216770233 42-43 0.22211646175901736 44-45 0.22825550584553933 46-47 0.23240666898975892 48-49 0.24249224141761638 50-51 0.2469357399945275 52-53 0.2691239993358139 54-55 0.2420537382685791 56-57 0.20583337815809968 58-59 0.20372856304272072 60-61 0.1991096632061947 62-63 0.21097848177347045 64-65 0.2015945143840726 66-67 0.19946046572542456 68-69 0.19361375707159412 70-71 0.20519024020617832 72-73 0.20697348634559662 74-75 0.21425263861961547 76-77 0.2200116466436384 78-79 0.2180237657013361 80-81 0.21594818412922628 82-83 0.20419629973502718 84-85 0.19463693108601443 86-87 0.1979987885619669 88-89 0.20340699406676005 90-91 0.2098968406725118 92-93 0.20100984351868958 94-95 0.19902196257638727 96-97 0.20048363973984487 98-99 0.21422340507634632 100-101 0.19282445140332702 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1710364.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.076737218336426 #Duplication Level Percentage of deduplicated Percentage of total 1 77.96831788190939 44.50177191101463 2 12.590642804316794 14.37265621503856 3 4.051596779317144 6.937557740632284 4 1.7611809777059153 4.0208985543381335 5 0.9552860295363637 2.7262304838097497 6 0.5789556844331298 1.9826940876871115 7 0.37386408237381347 1.4937259389517243 8 0.2746885202619831 1.2542659590309513 9 0.19948967916744326 1.024759799604936 >10 1.1084181336879892 11.72509130345967 >50 0.09078264780120668 3.6150761202100345 >100 0.04503308646161628 4.571397442827703 >500 0.001128271958803375 0.4046541304536027 >1k 4.1028071229213636E-4 0.5487302032322944 >5k 2.0514035614606818E-4 0.8204901097088024 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8061 0.47130318458526954 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5954 0.3481130332490628 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4213 0.24632183558587528 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2705 0.15815346908611266 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.9233543269152063E-5 0.0 22-23 0.0 0.0 0.0 5.8467086538304125E-5 0.0 24-25 0.0 0.0 0.0 5.8467086538304125E-5 0.0 26-27 0.0 0.0 0.0 3.215689759606727E-4 0.0 28-29 0.0 0.0 0.0 8.770062980745618E-4 0.0 30-31 0.0 0.0 0.0 0.0021048151153789485 0.0 32-33 0.0 0.0 0.0 0.003303390389414183 0.0 34-35 0.0 0.0 0.0 0.005203570701909067 0.0 36-37 0.0 0.0 0.0 0.00792229022594021 0.0 38-39 0.0 0.0 0.0 0.012658124235542842 0.0 40-41 0.0 0.0 0.0 0.017072389269184807 0.0 42-43 0.0 0.0 0.0 0.022217492884555566 0.0 44-45 0.0 0.0 0.0 0.025725518076853818 0.0 46-47 0.0 2.9233543269152063E-5 0.0 0.0312214242114544 0.0 48-49 0.0 5.8467086538304125E-5 0.0 0.036571162629709233 0.0 50-51 0.0 5.8467086538304125E-5 0.0 0.04276867380276947 0.0 52-53 0.0 5.8467086538304125E-5 0.0 0.05680077457196246 0.0 54-55 0.0 5.8467086538304125E-5 0.0 0.07904750099978718 0.0 56-57 0.0 5.8467086538304125E-5 0.0 0.10912881702374466 0.0 58-59 0.0 5.8467086538304125E-5 0.0 0.14836023209094673 0.0 60-61 0.0 5.8467086538304125E-5 0.0 0.19963586698503943 0.0 62-63 0.0 5.8467086538304125E-5 0.0 0.22930791340322879 0.0 64-65 0.0 5.8467086538304125E-5 0.0 0.2685100949271617 0.0 66-67 0.0 5.8467086538304125E-5 0.0 0.3465051883692594 0.0 68-69 0.0 5.8467086538304125E-5 0.0 0.4472439784747574 0.0 70-71 0.0 5.8467086538304125E-5 0.0 0.5559342923494648 0.0 72-73 0.0 5.8467086538304125E-5 0.0 0.6894731180029514 0.0 74-75 0.0 5.8467086538304125E-5 0.0 0.8244151537333573 0.0 76-77 0.0 5.8467086538304125E-5 0.0 0.9428987046032307 0.0 78-79 0.0 5.8467086538304125E-5 0.0 1.0689829767230834 0.0 80-81 0.0 5.8467086538304125E-5 0.0 1.2079300078813633 0.0 82-83 0.0 5.8467086538304125E-5 0.0 1.3805540808857062 0.0 84-85 0.0 5.8467086538304125E-5 0.0 1.6321671878032982 0.0 86-87 0.0 5.8467086538304125E-5 0.0 1.9504035398312873 0.0 88-89 0.0 5.8467086538304125E-5 0.0 2.318249214787028 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4775 0.0 43.12931 1 GTATCAA 8175 0.0 39.736626 1 TATCAAC 10345 0.0 31.296534 2 ATCAACG 10315 0.0 31.157103 3 TCAACGC 10410 0.0 30.78504 4 CAACGCA 10590 0.0 30.172869 5 AACGCAG 10900 0.0 29.238527 6 ACGCAGA 12410 0.0 25.680897 7 CGCAGAG 12360 0.0 25.593397 8 GTACATG 7895 0.0 25.54299 1 TACATGG 7975 0.0 24.262936 2 GCAGAGT 12820 0.0 23.971125 9 ACATGGG 7865 0.0 23.514215 3 GAGTACT 7110 0.0 22.612827 12-13 AGTACTT 7375 0.0 21.768097 12-13 TAGCGCG 110 0.0028611766 21.612644 4 GTACTTT 7535 0.0 21.178556 14-15 AGAGTAC 11570 0.0 20.936737 10-11 CATGGGG 3325 0.0 20.163134 4 ATTCGCG 155 2.6011548E-10 19.912828 80-81 >>END_MODULE