FastQCFastQC Report
Thu 26 May 2016
SRR937378_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937378_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1710364
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92300.5396512087485471No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74250.43411811754690816No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53770.31437752431646127No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18620.10886571513432229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56350.041.9122851
GTATCAA88500.034.7408071
ATCAACG111150.027.3508993
TATCAAC112300.027.1554092
TCAACGC112500.027.0226884
CAACGCA115500.026.3207995
AACGCAG118450.025.7833236
TAACGCG701.7815859E-523.74867276-77
ACGCAGA134900.022.572797
CGCAGAG135050.022.3718418
GAGTACT80250.022.05060212-13
GTACATG74500.021.6871031
CGGTATA1100.002877646821.591282
GCAGAGT142300.020.9983399
ACGCGAA804.994574E-520.78039278-79
GTACTTT84700.020.72020314-15
AGTACTT83050.020.70656812-13
TACATGG76550.020.5392192
TACCGTG1404.849637E-420.3592787
AGAGTAC124600.020.11146210-11
Produced by FastQC (version 0.11.5)