FastQCFastQC Report
Thu 26 May 2016
SRR937377_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937377_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1906423
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86230.45231304909770814No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63810.33471060724718493No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45440.2383521390583307No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30470.15982811789408752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48500.043.0445141
GTATCAA84400.040.2300721
ATCAACG105100.031.8842093
TATCAAC106150.031.7031542
TCAACGC106150.031.6135984
CAACGCA106700.031.3607235
AACGCAG110100.030.5464656
ACGCAGA125550.026.560457
CGCAGAG125000.026.4493038
GTACATG81250.026.0454391
TACATGG83450.025.061992
GCAGAGT129750.024.6363879
ACATGGG82950.024.1816143
GAGTACT69200.022.58301712-13
GTGTAGC8450.022.5111831
CATGGGG36500.021.7476774
AGAGTAC115150.021.2021510-11
AGTACTT74250.021.14302812-13
GTACTTT74300.020.87280514-15
CAGAGTA128000.020.0941310-11
Produced by FastQC (version 0.11.5)