FastQCFastQC Report
Thu 26 May 2016
SRR937377_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937377_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1906423
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101660.5332499660358693No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80610.4228337572511452No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59370.3114209176032811No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19890.10433151509397441No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60950.041.570571
GTATCAA96750.033.558471
CGTCGAT350.00839639827.14124544-45
ATCAACG121700.026.311143
TCAACGC123300.026.0082454
TATCAAC123900.025.997332
CAACGCA126200.025.4105915
AACGCAG129650.024.8018466
GTACATG87700.024.2292751
ATCACGC1601.8577684E-623.7541983
TACATGG89850.022.84212
ACATGGG90150.021.76483
ACGCAGA148850.021.5399917
CGCAGAG148100.021.488718
TACCTGG13650.021.2308582
GAGTACT85900.021.12325312-13
TATCACG1604.9847076E-520.7849242
AGTACTT89500.020.69818312-13
CATGGGG38200.020.645014
AACGCGA704.8565192E-420.35593476-77
Produced by FastQC (version 0.11.5)