##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937377_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1906423 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12097367688074 33.0 31.0 34.0 31.0 34.0 2 32.337530023504755 34.0 31.0 34.0 31.0 34.0 3 32.41883884111763 34.0 31.0 34.0 31.0 34.0 4 36.04898807872125 37.0 35.0 37.0 35.0 37.0 5 35.93368313328154 37.0 35.0 37.0 35.0 37.0 6 36.01470659974203 37.0 35.0 37.0 35.0 37.0 7 35.96523227006808 37.0 35.0 37.0 35.0 37.0 8 35.94189327342358 37.0 35.0 37.0 35.0 37.0 9 37.632640814761466 39.0 37.0 39.0 35.0 39.0 10-11 37.55432372563696 39.0 37.0 39.0 35.0 39.0 12-13 37.473463654183774 39.0 37.0 39.0 35.0 39.0 14-15 38.668400716944774 40.0 38.0 41.0 34.0 41.0 16-17 38.64088373881347 40.0 38.0 41.0 34.0 41.0 18-19 38.647901069175106 40.0 38.0 41.0 34.0 41.0 20-21 38.54439911813905 40.0 38.0 41.0 34.0 41.0 22-23 38.4207416192524 40.0 38.0 41.0 34.0 41.0 24-25 38.27856803028499 40.0 38.0 41.0 34.0 41.0 26-27 38.23733033015233 40.0 38.0 41.0 34.0 41.0 28-29 38.136127711426056 40.0 38.0 41.0 33.5 41.0 30-31 38.20663331275378 40.0 38.0 41.0 34.0 41.0 32-33 38.13611695830359 40.0 37.0 41.0 34.0 41.0 34-35 38.024162790734266 40.0 37.0 41.0 33.0 41.0 36-37 37.86778301562664 40.0 37.0 41.0 33.0 41.0 38-39 37.74353173456258 40.0 37.0 41.0 33.0 41.0 40-41 37.57492041378015 40.0 37.0 41.0 33.0 41.0 42-43 37.36733925262127 40.0 37.0 41.0 32.5 41.0 44-45 37.067908328844126 40.0 36.0 41.0 31.5 41.0 46-47 36.70773249168731 39.5 36.0 41.0 31.0 41.0 48-49 36.39502985433978 39.0 36.0 41.0 30.0 41.0 50-51 36.17858680890862 39.0 35.5 41.0 30.0 41.0 52-53 35.939580303007254 39.0 35.0 40.5 29.0 41.0 54-55 35.70829086724195 39.0 35.0 40.0 28.5 41.0 56-57 35.46371686661355 38.5 35.0 40.0 28.0 41.0 58-59 35.21763559294028 38.0 34.5 40.0 28.0 41.0 60-61 34.90251192940916 38.0 34.0 40.0 27.0 41.0 62-63 34.530331935777106 37.5 34.0 40.0 26.0 41.0 64-65 34.179986026186214 37.0 34.0 40.0 26.0 41.0 66-67 33.75462817013853 36.5 33.0 39.0 25.5 41.0 68-69 33.35483442027294 36.0 33.0 39.0 25.0 40.0 70-71 32.95396063727725 35.5 32.0 38.5 24.0 40.0 72-73 32.51015330805387 35.0 32.0 38.0 22.5 40.0 74-75 32.058311298174644 35.0 31.5 37.0 22.0 39.0 76-77 30.72590605547667 34.0 30.0 35.5 20.5 38.5 78-79 31.37911365945543 34.5 31.0 36.0 21.0 38.5 80-81 31.239878295635336 34.5 31.0 36.0 20.0 37.5 82-83 30.912104239195603 34.0 31.0 35.5 19.5 37.0 84-85 30.544505075736076 34.0 31.0 35.0 18.0 36.5 86-87 30.158311927625718 34.0 30.5 35.0 16.0 36.0 88-89 29.761108893461735 34.0 30.0 35.0 9.0 36.0 90-91 29.313190199656635 34.0 29.5 35.0 4.5 35.0 92-93 28.937053843769196 34.0 29.0 35.0 2.0 35.0 94-95 28.501972804566456 34.0 29.0 35.0 2.0 35.0 96-97 28.001183630285617 33.0 29.0 35.0 2.0 35.0 98-99 27.4716778490398 33.0 27.5 35.0 2.0 35.0 100-101 25.536507899873218 31.5 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24345832756020513 1101 2 0.25422392065172517 1101 3 0.1960793595413577 1101 4 0.09361419310673114 1101 5 0.08840178553006695 1101 6 0.08483482777563722 1101 7 0.1163244114378017 1101 8 0.1328017034392417 1101 9 0.16253524673129505 1101 10-11 0.1262067773255211 1101 12-13 0.074945272913439 1101 14-15 0.08033979518261702 1101 16-17 0.12498501350977875 1101 18-19 0.050020286077014475 1101 20-21 0.17997487612135643 1101 22-23 0.14511960991440986 1101 24-25 0.3047716023918525 1101 26-27 0.20641201768975037 1101 28-29 0.2410729264098137 1101 30-31 0.2593779441793629 1101 32-33 0.1801510447490955 1101 34-35 0.15774484604526862 1101 36-37 0.2528778707281205 1101 38-39 0.1848570669909222 1101 40-41 0.1685908125081923 1101 42-43 0.3332321352883625 1101 44-45 0.35434307763425466 1101 46-47 0.4067560394402321 1101 48-49 0.5148821900721288 1101 50-51 0.29571402736743835 1101 52-53 0.3093744900915354 1101 54-55 0.2711236797458767 1101 56-57 0.4489597027223553 1101 58-59 0.3061568807466841 1101 60-61 0.2640109589237838 1101 62-63 0.40490147147296085 1101 64-65 0.37649953292899596 1101 66-67 0.30585283653828554 1101 68-69 0.3762209343060263 1101 70-71 0.25916160446285375 1101 72-73 0.05651038396715791 1101 74-75 0.2054488755448105 1101 76-77 0.14126439904904586 1101 78-79 0.1359672101333267 1101 80-81 0.3144210560363838 1101 82-83 0.23823993411790667 1101 84-85 0.28262978604428923 1101 86-87 0.274073581556852 1101 88-89 0.3397315621067918 1101 90-91 0.20600682213584065 1101 92-93 0.2848465962651261 1101 94-95 0.20639639996147352 1101 96-97 0.11002218021155485 1101 98-99 0.3711104820303426 1101 100-101 0.8159211565254125 1102 1 0.09251632457027625 1102 2 0.0611871959854966 1102 3 0.05664915820447192 1102 4 0.03328210810035159 1102 5 -0.003965000136744834 1102 6 -0.02555714364937245 1102 7 -0.03144745299363194 1102 8 0.005959707683921067 1102 9 0.03138184010354195 1102 10-11 0.06014150477454905 1102 12-13 0.09308580101687625 1102 14-15 -0.003186943771481765 1102 16-17 -0.06977334053813422 1102 18-19 0.055236302473950616 1102 20-21 0.03720582234173264 1102 22-23 0.0026874230814968314 1102 24-25 0.036741602913416216 1102 26-27 0.03907710072034121 1102 28-29 -0.007415051347244628 1102 30-31 0.08618365476579015 1102 32-33 0.039392350095425854 1102 34-35 7.849449891850213E-4 1102 36-37 -0.05512217712865919 1102 38-39 -0.1526602460446469 1102 40-41 -0.04507125460983019 1102 42-43 0.03734322062244644 1102 44-45 0.14864215603582664 1102 46-47 0.07180738360851535 1102 48-49 0.08258403403543468 1102 50-51 -0.01760896690488778 1102 52-53 0.07587110936439956 1102 54-55 -0.0066897621426207365 1102 56-57 -0.11447924145213761 1102 58-59 0.06839985379003366 1102 60-61 -0.023938962682684917 1102 62-63 -0.09218272264390492 1102 64-65 0.030246018679541464 1102 66-67 -0.10816467942130004 1102 68-69 -0.1431075077890327 1102 70-71 -0.06604707264644105 1102 72-73 -0.13536001834581413 1102 74-75 -0.15576044688340573 1102 76-77 -0.18358628682664246 1102 78-79 -0.05434803339511873 1102 80-81 -0.11903454267544333 1102 82-83 -0.09298271530511215 1102 84-85 -0.08626615964423223 1102 86-87 -0.07743053309904724 1102 88-89 0.02147127986014752 1102 90-91 -0.009698131771152418 1102 92-93 -0.14258157041036057 1102 94-95 -0.052058876277836674 1102 96-97 0.04444837447685401 1102 98-99 0.142114799797195 1102 100-101 0.5174272247104987 1103 1 0.04017272374821346 1103 2 0.03893119381825727 1103 3 -0.008413827174329924 1103 4 0.05880320746292256 1103 5 -0.09738226772904568 1103 6 -0.0665346063369725 1103 7 -0.07236303108158637 1103 8 -8.003177124962235E-4 1103 9 0.017736012056566608 1103 10-11 0.01132742546222687 1103 12-13 -0.04086067665410553 1103 14-15 0.023088454015052662 1103 16-17 -0.08051971992394868 1103 18-19 -0.026352517990972046 1103 20-21 -0.18812787073766657 1103 22-23 -0.10351476467601373 1103 24-25 -0.03666667589514816 1103 26-27 -0.09043568310451633 1103 28-29 -0.13142047090475018 1103 30-31 -0.1263999852827098 1103 32-33 -0.12757741115415655 1103 34-35 -0.15467391562588517 1103 36-37 -0.07347178239984231 1103 38-39 -0.10880149846480691 1103 40-41 -0.019279159116969424 1103 42-43 -0.030429178407992197 1103 44-45 0.02540089262612355 1103 46-47 0.03285659592161494 1103 48-49 0.24979403166592817 1103 50-51 0.11351491584078133 1103 52-53 0.18325741056021627 1103 54-55 -0.009469914090303178 1103 56-57 0.10500973414415427 1103 58-59 0.09303978217185005 1103 60-61 -0.14289649743390243 1103 62-63 -0.05087593895996889 1103 64-65 0.06609518309140583 1103 66-67 0.10795916133776018 1103 68-69 0.15383533822346607 1103 70-71 0.09447683251642047 1103 72-73 0.29716459219194036 1103 74-75 0.1708525961995946 1103 76-77 0.17827323038585874 1103 78-79 0.25057089777935104 1103 80-81 0.13534256053382876 1103 82-83 0.011484878157975942 1103 84-85 0.04768825897094686 1103 86-87 0.08839445433235582 1103 88-89 0.09714555729811636 1103 90-91 0.08666139256081351 1103 92-93 0.13013007765867712 1103 94-95 0.23497175459093356 1103 96-97 0.04505270771319303 1103 98-99 0.23358708371132764 1103 100-101 0.4685335534057131 1104 1 0.05072210191146098 1104 2 0.07191407240038217 1104 3 0.05433793400263198 1104 4 0.008953849709513406 1104 5 0.02946152484516773 1104 6 -0.025650101673292625 1104 7 -0.046214499079049176 1104 8 -0.042865203295860965 1104 9 0.007097847438373606 1104 10-11 0.02293616234411644 1104 12-13 0.013671890291249156 1104 14-15 -0.06369551638258031 1104 16-17 -0.025909602983716695 1104 18-19 -0.10953197936297698 1104 20-21 -0.29125812746683977 1104 22-23 -0.0794615561693135 1104 24-25 -0.06163832685901127 1104 26-27 -0.11677597174522703 1104 28-29 -0.06244315823864355 1104 30-31 0.006846379077742881 1104 32-33 -0.03360063268041813 1104 34-35 -0.025490913111134716 1104 36-37 -0.07914140542213488 1104 38-39 -0.024757013872026334 1104 40-41 0.06155703982069127 1104 42-43 0.024858626017184804 1104 44-45 0.1772949913846702 1104 46-47 0.14323068530784155 1104 48-49 0.12907208358058142 1104 50-51 0.08780736884474294 1104 52-53 0.24390776950064463 1104 54-55 0.15641541949256776 1104 56-57 0.1239580899000643 1104 58-59 0.2489662964598338 1104 60-61 0.19076760535502757 1104 62-63 0.2338813792912049 1104 64-65 0.2040116781411072 1104 66-67 0.27810182385565696 1104 68-69 0.11074019819506731 1104 70-71 0.02082570507579362 1104 72-73 0.11364409504674455 1104 74-75 0.021334364030018094 1104 76-77 0.17261521223215937 1104 78-79 0.21621238175836766 1104 80-81 0.2233907247909812 1104 82-83 0.10669729883040802 1104 84-85 0.220442753739178 1104 86-87 0.3675501209344283 1104 88-89 0.3602960838644229 1104 90-91 0.3752380020087216 1104 92-93 0.29903415070966943 1104 94-95 0.11421370246996787 1104 96-97 0.2235219555482395 1104 98-99 0.2740834699920889 1104 100-101 0.7588262097711862 1105 1 -0.009786135334110213 1105 2 0.06514514294526208 1105 3 0.043443004768619176 1105 4 0.038833020512207383 1105 5 -0.0986065653642072 1105 6 -0.078679841369798 1105 7 0.002500783329843159 1105 8 -0.028776725839747996 1105 9 -0.015442103389560202 1105 10-11 -0.02572325165925804 1105 12-13 -0.06383971912406139 1105 14-15 0.0036683053625807815 1105 16-17 0.09925401865812944 1105 18-19 0.04555832089823042 1105 20-21 0.07927913774323514 1105 22-23 0.041511935059148186 1105 24-25 0.009327039844279739 1105 26-27 0.02122715373796069 1105 28-29 -0.056446648833670565 1105 30-31 0.022746842037975057 1105 32-33 0.017702792957749125 1105 34-35 -0.0940077092390581 1105 36-37 -0.1917208654421927 1105 38-39 -0.19266773616001132 1105 40-41 -0.04980492174263418 1105 42-43 -0.17263520386824638 1105 44-45 -0.03571235373689774 1105 46-47 0.042930766395720354 1105 48-49 0.09231972186177018 1105 50-51 0.18820476238690986 1105 52-53 0.0945954230158037 1105 54-55 0.1409957395713164 1105 56-57 0.21911182047349342 1105 58-59 0.10242022292678854 1105 60-61 0.17873435735871368 1105 62-63 0.22737782476226442 1105 64-65 0.21804409047197026 1105 66-67 0.037502273242225215 1105 68-69 0.18669014797252004 1105 70-71 0.2459669203395194 1105 72-73 0.11954116262430148 1105 74-75 0.1559964998476815 1105 76-77 0.11177038751534596 1105 78-79 0.21893970420208575 1105 80-81 0.14549324961012644 1105 82-83 0.07926030521160854 1105 84-85 -0.02026863204514484 1105 86-87 0.2477906855672849 1105 88-89 0.16716864527737485 1105 90-91 0.10347942197900295 1105 92-93 0.014913604001907999 1105 94-95 0.03162288778665001 1105 96-97 0.10264249635440237 1105 98-99 0.23250832718339254 1105 100-101 0.5130665037176882 1106 1 -0.2947591197011832 1106 2 -0.12135069716349278 1106 3 -0.1529931256291306 1106 4 -0.04088783228700521 1106 5 -0.2081269948611535 1106 6 -0.23510766919245896 1106 7 -0.22193201743416324 1106 8 -0.1828447725406548 1106 9 -0.28891408542502717 1106 10-11 -0.3193170406447976 1106 12-13 -0.35323801903200547 1106 14-15 -0.3362999899853918 1106 16-17 -0.18945942635129143 1106 18-19 -0.25871922601346853 1106 20-21 -0.25576579510484265 1106 22-23 -0.1152544485456346 1106 24-25 -0.11758868256578126 1106 26-27 -0.14428016371292784 1106 28-29 -0.14104214565460182 1106 30-31 -0.07769624875692216 1106 32-33 -0.1758660452839962 1106 34-35 -0.3538473361824188 1106 36-37 -0.3538681135472288 1106 38-39 -0.24740300016469519 1106 40-41 -0.3833124800972669 1106 42-43 -0.6691303771898802 1106 44-45 -0.577977730708227 1106 46-47 -0.30259810333559045 1106 48-49 -0.2913425956321447 1106 50-51 -0.24672488081745314 1106 52-53 -0.20686774452413204 1106 54-55 -0.289090017855834 1106 56-57 -0.30606046871422876 1106 58-59 -0.3505105215517901 1106 60-61 -0.20073429934485887 1106 62-63 -0.29456742986437945 1106 64-65 -0.33531447322555863 1106 66-67 -0.3512441884841948 1106 68-69 -0.2625087395684531 1106 70-71 -0.1382781906052486 1106 72-73 0.02619537542833683 1106 74-75 -0.25256493920797496 1106 76-77 -0.21621563576627878 1106 78-79 -0.17169236696776125 1106 80-81 -0.12697597063611354 1106 82-83 -0.3118065756873101 1106 84-85 -0.3266676374568789 1106 86-87 -0.13636204309767663 1106 88-89 -0.14207990714929863 1106 90-91 -0.18951060202533299 1106 92-93 -0.20598705332113454 1106 94-95 -0.8105394598202302 1106 96-97 -0.41395749160041007 1106 98-99 -0.2666826019306825 1106 100-101 0.21561373805679196 1107 1 0.022918145772479193 1107 2 -0.020704058999250208 1107 3 -0.06873581014821184 1107 4 -0.033748358131198586 1107 5 -0.12528946723342216 1107 6 -0.07228413676881473 1107 7 -0.08051901384149573 1107 8 -0.11035366888227571 1107 9 -0.08776609467551566 1107 10-11 0.00530801162387462 1107 12-13 0.04023479699079502 1107 14-15 -0.10031247473148142 1107 16-17 -0.12464947098234092 1107 18-19 -0.007952263228759193 1107 20-21 0.0740116683497476 1107 22-23 0.09056124144353106 1107 24-25 -0.09268095277563049 1107 26-27 -0.051632339369810154 1107 28-29 -0.043168307299211506 1107 30-31 -0.01777032093463049 1107 32-33 -0.00281673352375833 1107 34-35 0.04357635155385964 1107 36-37 0.034923242707520785 1107 38-39 0.09883984222712172 1107 40-41 0.05128767521537014 1107 42-43 0.15275686328016036 1107 44-45 0.08483588133447029 1107 46-47 0.079422539590432 1107 48-49 0.15041996805030777 1107 50-51 0.17592441558218752 1107 52-53 0.030893598844784265 1107 54-55 0.006339459969446182 1107 56-57 0.03763486251982329 1107 58-59 -0.0806969607110517 1107 60-61 0.040227166908778145 1107 62-63 0.06848629806544437 1107 64-65 -0.0468260619372316 1107 66-67 -0.14777578863097318 1107 68-69 -0.025112423549920493 1107 70-71 -0.0782910337650975 1107 72-73 -0.06962771751177144 1107 74-75 -0.10906236204234432 1107 76-77 -0.09784810378638653 1107 78-79 -0.06427981565912688 1107 80-81 -0.03078646362570936 1107 82-83 0.052746623155524475 1107 84-85 0.09806675234369067 1107 86-87 -5.952296520383982E-4 1107 88-89 0.010340239117649475 1107 90-91 0.12247844502073946 1107 92-93 0.2115708475689253 1107 94-95 0.14055466219405233 1107 96-97 0.13133747439295362 1107 98-99 0.25885000518809775 1107 100-101 0.6304453475906016 1108 1 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96-97 -0.3046439263022549 2316 98-99 -0.31543197844639437 2316 100-101 -0.7157387068615932 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 7.0 4 10.0 5 41.0 6 80.0 7 171.0 8 477.0 9 908.0 10 1524.0 11 2509.0 12 3800.0 13 5561.0 14 7046.0 15 9852.0 16 13491.0 17 19374.0 18 17041.0 19 9893.0 20 8674.0 21 8748.0 22 8951.0 23 9959.0 24 11615.0 25 13798.0 26 16928.0 27 21059.0 28 26629.0 29 33976.0 30 42556.0 31 54378.0 32 69188.0 33 91202.0 34 124941.0 35 179724.0 36 275491.0 37 424912.0 38 356801.0 39 35107.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.45758686655838 20.143804467910346 15.68056514106221 21.718043524469067 2 15.604616603975089 20.95857005501927 39.98367623554688 23.453137105458758 3 19.075409811988212 25.264592380599687 30.44607623806469 25.213921569347413 4 13.002937962876024 17.586181031177237 37.30210976262875 32.10877124331798 5 13.474779907870852 37.96161635239924 34.171414780537454 14.392188959192453 6 31.400743696440927 37.2704273920321 17.202583057380235 14.126245854146745 7 28.127283399329528 33.49728785269586 20.641693894796695 17.733734853177914 8 26.512321766995044 35.473082311742985 19.362282137804673 18.65231378345729 9 26.413550403032275 16.845369574328465 20.418920669756922 36.32215935288233 10-11 24.7817771816643 27.417787133285742 27.209806008425204 20.590629676624758 12-13 25.820818639091588 24.674182564774 28.020456797486727 21.484541998647682 14-15 22.563224426058646 25.83728794711352 26.644060630825372 24.955426996002462 16-17 21.727654355827642 29.39804020408902 27.09325265169377 21.781052788389566 18-19 21.65710338156852 27.360008770351595 30.069139954773938 20.913747893305946 20-21 22.34184123880167 26.43573855330113 30.401358984863275 20.821061223033922 22-23 22.0861343055639 26.443232332663573 30.24247451946896 21.228158842303568 24-25 22.014258145769634 26.627829454070696 30.313535481969073 21.044376918190597 26-27 21.80068385312973 26.90844304471745 30.134412783023112 21.15646031912971 28-29 21.488346792204112 26.95826373092015 30.36173321614687 21.19165626072887 30-31 21.674701784441332 26.66968453485926 30.393202348062314 21.26241133263709 32-33 21.484770558467496 26.837178012743767 30.280276276638574 21.397775152150167 34-35 22.0496448060058 26.816320407380733 29.980308672314592 21.153726114298873 36-37 22.07327544831341 26.591186740822998 29.91093791881445 21.42459989204914 38-39 21.785642535785605 26.920310969811002 30.080522528316116 21.213523966087276 40-41 21.960288980986906 26.786001847438897 30.018442916393685 21.235266255180512 42-43 21.894931504154343 26.83649526857323 29.767107567003954 21.501465660268476 44-45 21.917696125151657 27.095036096396235 29.459149412276286 21.528118366175818 46-47 22.128457325577795 27.355314114443647 28.941478360258976 21.57475019971958 48-49 22.312204584187246 27.441050595801663 28.68930452475657 21.557440295254516 50-51 22.238112947651175 27.50153035291748 28.486883551027237 21.773473148404104 52-53 22.484988903302153 27.709695067673856 28.295687788072215 21.509628240951773 54-55 22.25114783025593 27.617926346881045 28.345938965276858 21.78498685758617 56-57 22.24026357214532 27.909676918501297 28.279007334678607 21.571052174674772 58-59 22.441478097987698 27.445299390534 28.367891071393913 21.74533144008439 60-61 22.289386982846935 27.522118648379713 28.706719337733546 21.481775031039806 62-63 22.224369932591035 27.564003371759572 28.717760958612022 21.493865737037375 64-65 22.128352417065887 27.52463645266554 28.53002717655001 21.816983953718562 66-67 22.144403419388038 27.74423619522005 28.31512208990345 21.79623829548846 68-69 22.087097144757486 27.643550250914934 28.471514454032498 21.79783815029508 70-71 22.099187850755055 27.6143856846041 28.44617904840636 21.840247416234487 72-73 22.264325527086868 27.586179765025793 28.251431215124455 21.89806349276288 74-75 22.154999179090893 27.859268378528796 28.05788641870141 21.927846023678903 76-77 22.159064383927387 27.713812726766307 28.21089548332138 21.916227405984927 78-79 22.3114439974759 27.743764106916462 28.070239395978753 21.874552499628884 80-81 22.120615414312564 28.123847645564492 28.021456937940847 21.734080002182097 82-83 22.2155197058353 28.00531363842605 27.914278895203825 21.864887760534828 84-85 22.132191911959485 27.848583320875264 27.89865164354711 22.120573123618136 86-87 22.16021837755839 27.706810083596352 28.090224467497503 22.042747071347755 88-89 22.25683911702702 27.618477116568567 27.9545253073426 22.170158459061813 90-91 22.19986848307264 27.69758556010237 28.049591376854227 22.052954579970766 92-93 22.184885939287565 27.463928027582924 28.132650229726547 22.218535803402965 94-95 22.06191910189921 27.44765983205196 28.094735533509613 22.39568553253921 96-97 22.179626452261644 27.36813918002458 28.03617035673615 22.416064010977628 98-99 21.909314197965557 27.472602407395026 27.955051818397596 22.663031576241828 100-101 21.809655862735408 27.55367078699667 28.20715806349736 22.429515286770563 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 619.0 1 443.5 2 315.0 3 408.0 4 362.0 5 334.5 6 622.0 7 1460.5 8 2509.0 9 3022.5 10 2771.5 11 2366.0 12 2395.0 13 2596.5 14 2799.5 15 3004.0 16 3183.5 17 3305.5 18 3476.0 19 3746.0 20 4098.0 21 4681.5 22 5541.0 23 6478.0 24 7575.5 25 9348.0 26 11953.0 27 14831.5 28 17097.0 29 19998.5 30 23842.5 31 27487.5 32 31610.0 33 35681.0 34 40414.5 35 44313.5 36 48691.5 37 54090.5 38 57022.0 39 57947.5 40 59193.0 41 62362.5 42 65387.5 43 67878.0 44 70107.0 45 72119.0 46 71600.5 47 71422.0 48 75468.0 49 75282.5 50 71283.0 51 67510.0 52 62718.0 53 59682.5 54 57389.5 55 52354.0 56 46540.5 57 41590.5 58 37432.0 59 33256.5 60 28255.5 61 22759.0 62 17703.0 63 13892.5 64 10833.0 65 8372.0 66 6094.5 67 4812.5 68 3469.5 69 2074.0 70 1455.5 71 1100.5 72 864.5 73 637.0 74 460.0 75 367.0 76 264.5 77 130.0 78 72.0 79 44.5 80 20.5 81 13.5 82 8.5 83 4.0 84 1.0 85 1.0 86 1.0 87 1.0 88 1.0 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.055496602800113086 2 0.0 3 0.0 4 0.0 5 0.021139065149759523 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0033570723811032492 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.654952232531814E-4 24-25 2.0981702381895308E-4 26-27 0.0010228579911173963 28-29 0.0031210282293069273 30-31 0.0 32-33 2.6227127977369135E-5 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.014975690075077775 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 5.245425595473827E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.1963404763790615E-4 84-85 0.0014687191667326716 86-87 0.0 88-89 0.0 90-91 0.010726895342743977 92-93 0.001363810654823195 94-95 0.0 96-97 0.0 98-99 2.884984077510605E-4 100-101 0.013900377828005643 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1906423.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.50158646538973 #Duplication Level Percentage of deduplicated Percentage of total 1 75.09037804564007 40.17454353727614 2 14.021370655991802 15.003311490296475 3 4.626378120788281 7.425557071528242 4 2.0883957869560676 4.469299510791427 5 1.1120513754087908 2.9748256407694496 6 0.6728989782391424 2.1600697720040323 7 0.43445402881489553 1.6270785851513938 8 0.30719924608976423 1.3148517621419247 9 0.2273546033880971 1.09474487743254 >10 1.2589323067127112 12.401161093138462 >50 0.10376524767226102 3.878491998138614 >100 0.05407434482681722 5.216362513016571 >500 0.0020604446035368176 0.7078793753345027 >1k 3.9246563876891747E-4 0.2833617254768739 >5k 1.9623281938445874E-4 0.7348087130836014 >10k+ 9.811640969222937E-5 0.5336523344197666 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10166 0.5332499660358693 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8061 0.4228337572511452 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5937 0.3114209176032811 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1989 0.10433151509397441 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 2.6227127977369135E-5 0.0 24-25 0.0 0.0 0.0 5.245425595473827E-5 0.0 26-27 0.0 0.0 0.0 2.884984077510605E-4 0.0 28-29 0.0 0.0 0.0 7.343595833663359E-4 0.0 30-31 0.0 0.0 0.0 0.0019408074703253161 0.0 32-33 0.0 0.0 0.0 0.004117659092446954 0.0 34-35 0.0 0.0 0.0 0.0063731920985007 0.0 36-37 0.0 0.0 0.0 0.009441766071852889 0.0 38-39 0.0 0.0 0.0 0.014818327307213561 0.0 40-41 0.0 0.0 0.0 0.019775254494936327 0.0 42-43 0.0 0.0 0.0 0.025571449777934907 0.0 44-45 0.0 0.0 0.0 0.030134970045997137 0.0 46-47 0.0 0.0 0.0 0.03629834512067888 0.0 48-49 0.0 0.0 0.0 0.04143886220424323 0.0 50-51 0.0 0.0 0.0 0.0470776947193776 0.0 52-53 0.0 0.0 0.0 0.06121411669917956 0.0 54-55 0.0 0.0 0.0 0.08206468344118803 0.0 56-57 0.0 0.0 0.0 0.11337987424616677 0.0 58-59 0.0 0.0 0.0 0.15127807417346517 0.0 60-61 0.0 0.0 0.0 0.20150302425012706 0.0 62-63 0.0 0.0 0.0 0.2318215841919658 0.0 64-65 0.0 0.0 0.0 0.27027055380678894 0.0 66-67 0.0 0.0 0.0 0.34680131324475205 0.0 68-69 0.0 0.0 0.0 0.45045092301131495 0.0 70-71 0.0 0.0 0.0 0.5550709365130404 0.0 72-73 0.0 0.0 0.0 0.6802005640930685 0.0 74-75 0.0 0.0 0.0 0.8075594975511731 0.0 76-77 0.0 0.0 0.0 0.9256602548332663 0.0 78-79 0.0 0.0 0.0 1.0460952265053454 0.0 80-81 0.0 0.0 0.0 1.1775718190558968 0.0 82-83 0.0 0.0 0.0 1.3399177412358116 0.0 84-85 0.0 0.0 0.0 1.579502555309079 0.0 86-87 0.0 0.0 0.0 1.8751085147420063 0.0 88-89 0.0 0.0 0.0 2.230905732882996 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6095 0.0 41.57057 1 GTATCAA 9675 0.0 33.55847 1 CGTCGAT 35 0.008396398 27.141245 44-45 ATCAACG 12170 0.0 26.31114 3 TCAACGC 12330 0.0 26.008245 4 TATCAAC 12390 0.0 25.99733 2 CAACGCA 12620 0.0 25.410591 5 AACGCAG 12965 0.0 24.801846 6 GTACATG 8770 0.0 24.229275 1 ATCACGC 160 1.8577684E-6 23.754198 3 TACATGG 8985 0.0 22.8421 2 ACATGGG 9015 0.0 21.7648 3 ACGCAGA 14885 0.0 21.539991 7 CGCAGAG 14810 0.0 21.48871 8 TACCTGG 1365 0.0 21.230858 2 GAGTACT 8590 0.0 21.123253 12-13 TATCACG 160 4.9847076E-5 20.784924 2 AGTACTT 8950 0.0 20.698183 12-13 CATGGGG 3820 0.0 20.64501 4 AACGCGA 70 4.8565192E-4 20.355934 76-77 >>END_MODULE