FastQCFastQC Report
Thu 26 May 2016
SRR937376_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937376_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2050129
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91570.4466548202576521No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68580.33451553536387224No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47590.23213173414941204No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32950.16072159361679192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57050.041.6459161
GTATCAA95150.036.855781
ATCAACG118200.029.5823963
TCAACGC119000.029.3079814
TATCAAC120250.028.9983582
CAACGCA120000.028.9442485
AACGCAG123450.028.0549476
GTACATG87550.026.1063981
TACATGG88750.025.3195022
ACGCAGA138750.024.7216387
CGCAGAG139750.024.475558
ACATGGG88150.024.3067683
GCAGAGT146350.022.7869159
GAGTACT77650.022.42031912-13
AGAGTAC127150.020.88382510-11
AGTACTT83200.020.8676412-13
GTACTTT83750.020.7564214-15
CATGGGA49400.020.295154
CGCGGAA1900.019.99460682-83
CATGGGG39350.019.6824724
Produced by FastQC (version 0.11.5)