FastQCFastQC Report
Thu 26 May 2016
SRR937376_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937376_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2050129
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107390.5238206961610709No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86480.42182711429378345No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60600.29559115548338666No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21250.10365201409277172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65650.041.1932141
GTATCAA104800.035.010971
ATCAACG132400.027.5880853
TATCAAC134300.027.3392582
TCAACGC134050.027.2130784
CAACGCA136250.026.8085355
AACGCAG138850.026.3743116
GTACATG91550.025.1266821
GCGTATC1000.001648453623.7639221
TACATGG94900.023.2238942
ACATGGG92750.023.1989083
CGCAGAG157500.023.1664358
ACGCAGA157650.023.1443967
GAGTACT92500.021.87995512-13
GCAGAGT167750.021.4676889
AGTACTT96250.021.34833312-13
GTAAGGT6150.020.853164
GTACTTT98300.020.70474814-15
AGAGTAC145700.020.62431710-11
TAAGGTG7400.019.2562985
Produced by FastQC (version 0.11.5)