##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937376_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2050129 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.203440856648534 33.0 31.0 34.0 31.0 34.0 2 32.403379006882005 34.0 31.0 34.0 31.0 34.0 3 32.45504551177024 34.0 31.0 34.0 31.0 34.0 4 36.10420661333994 37.0 35.0 37.0 35.0 37.0 5 36.024284813297115 37.0 35.0 37.0 35.0 37.0 6 36.06748014393241 37.0 35.0 37.0 35.0 37.0 7 36.012449460497365 37.0 35.0 37.0 35.0 37.0 8 35.98498679839171 37.0 35.0 37.0 35.0 37.0 9 37.69531819705004 39.0 37.0 39.0 35.0 39.0 10-11 37.61441938531673 39.0 37.0 39.0 35.0 39.0 12-13 37.51654505643303 39.0 37.0 39.0 35.0 39.0 14-15 38.710351153512775 40.0 38.0 41.0 34.5 41.0 16-17 38.67586600648057 40.0 38.0 41.0 34.5 41.0 18-19 38.672888145087455 40.0 38.0 41.0 34.0 41.0 20-21 38.581089287552146 40.0 38.0 41.0 34.0 41.0 22-23 38.436601062664835 40.0 38.0 41.0 34.0 41.0 24-25 38.37579074292398 40.0 38.0 41.0 34.0 41.0 26-27 38.334752105843094 40.0 38.0 41.0 34.0 41.0 28-29 38.20890295196058 40.0 38.0 41.0 34.0 41.0 30-31 38.27782007863895 40.0 38.0 41.0 34.0 41.0 32-33 38.22466439916708 40.0 38.0 41.0 34.0 41.0 34-35 38.10313448568358 40.0 37.5 41.0 33.5 41.0 36-37 37.93384123633196 40.0 37.0 41.0 33.0 41.0 38-39 37.8206244582658 40.0 37.0 41.0 33.0 41.0 40-41 37.6453435369189 40.0 37.0 41.0 33.0 41.0 42-43 37.432515466099936 40.0 37.0 41.0 33.0 41.0 44-45 37.15052223543006 40.0 36.5 41.0 32.0 41.0 46-47 36.79383785117912 40.0 36.0 41.0 31.0 41.0 48-49 36.468561734407935 39.0 36.0 41.0 30.0 41.0 50-51 36.22932166707558 39.0 35.5 41.0 30.0 41.0 52-53 35.98748859218128 39.0 35.0 40.5 29.0 41.0 54-55 35.75768671142157 39.0 35.0 40.0 28.5 41.0 56-57 35.49867593697763 38.5 35.0 40.0 28.0 41.0 58-59 35.22858537194489 38.0 34.5 40.0 28.0 41.0 60-61 34.915797493718685 38.0 34.0 40.0 27.0 41.0 62-63 34.55323982051861 37.5 34.0 40.0 26.0 41.0 64-65 34.16045331781561 37.0 34.0 40.0 26.0 41.0 66-67 33.74871410530752 36.0 33.0 39.0 25.5 40.5 68-69 33.32014400069459 36.0 33.0 39.0 25.0 40.0 70-71 32.920495734658644 35.0 32.0 38.5 24.0 40.0 72-73 32.45397704242026 35.0 32.0 38.0 22.5 40.0 74-75 31.975497639416837 35.0 31.0 37.0 21.5 39.0 76-77 30.707072823222344 34.0 29.5 35.5 20.5 38.5 78-79 31.32271457064409 34.0 31.0 36.0 21.0 38.5 80-81 31.137759380019503 34.0 31.0 36.0 20.0 37.5 82-83 30.79486217696545 34.0 31.0 35.0 18.5 37.0 84-85 30.433363705405853 34.0 31.0 35.0 18.0 36.5 86-87 30.015328059844038 34.0 30.0 35.0 13.5 36.0 88-89 29.561859278123475 34.0 30.0 35.0 7.0 36.0 90-91 29.049252754338873 34.0 29.0 35.0 3.5 35.0 92-93 28.69238399144639 34.0 29.0 35.0 2.0 35.0 94-95 28.24609573348799 33.5 29.0 35.0 2.0 35.0 96-97 27.763998997136277 33.0 28.0 35.0 2.0 35.0 98-99 27.01505222354301 33.0 26.5 35.0 2.0 35.0 100-101 24.5329401223045 30.5 21.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.011149889810432967 1101 2 -0.00563112407321853 1101 3 0.043552015842770686 1101 4 0.038978205960781054 1101 5 0.059658721936074244 1101 6 0.021807475585085 1101 7 0.010339859433230458 1101 8 -0.008361438711666835 1101 9 0.06999314103273946 1101 10-11 0.061759481309742625 1101 12-13 0.02631553763711736 1101 14-15 -0.0010278549923654623 1101 16-17 0.012731534210807638 1101 18-19 0.02196927676236271 1101 20-21 0.07474855672028724 1101 22-23 0.03395530292192461 1101 24-25 0.012103911012431467 1101 26-27 -0.04788240422222856 1101 28-29 -0.16168467399608488 1101 30-31 -0.1028514777044478 1101 32-33 0.04287347861986035 1101 34-35 0.08054598093176679 1101 36-37 0.08279700575576499 1101 38-39 0.04848919916950223 1101 40-41 0.018946944536573085 1101 42-43 0.12270914601564442 1101 44-45 0.064913546623238 1101 46-47 0.22007738481742223 1101 48-49 0.21371822434351628 1101 50-51 0.3149519790981046 1101 52-53 0.3454498123366321 1101 54-55 0.2600195477401783 1101 56-57 0.40143631427659443 1101 58-59 0.5322824655419893 1101 60-61 0.2737923657520298 1101 62-63 0.24654612773907303 1101 64-65 0.25519313895398454 1101 66-67 0.15755895209029092 1101 68-69 0.06655204924170022 1101 70-71 0.051597536445434855 1101 72-73 0.20652227330813133 1101 74-75 0.17420501698497404 1101 76-77 0.211096997232616 1101 78-79 0.08318336611079147 1101 80-81 0.0684172132151879 1101 82-83 0.16846651616055652 1101 84-85 0.1251690652711126 1101 86-87 0.20707533053285587 1101 88-89 0.21317937071206927 1101 90-91 0.13134958162938304 1101 92-93 0.1156574343523431 1101 94-95 0.2940262818978141 1101 96-97 0.26080701556658425 1101 98-99 0.41823501533542284 1101 100-101 1.1327812683010308 1102 1 -0.03634920925570384 1102 2 0.00940438743092642 1102 3 0.04379436454793506 1102 4 0.010603623888648883 1102 5 0.03351473712984898 1102 6 -0.017477744111431548 1102 7 -0.030364831250686564 1102 8 -0.03363989242801324 1102 9 -0.06815056680353138 1102 10-11 -0.02813210473888006 1102 12-13 -0.11543867131692309 1102 14-15 -0.04254861784674091 1102 16-17 -0.18029426356156364 1102 18-19 -0.1696543606444081 1102 20-21 -0.027290139938266122 1102 22-23 -0.05741510481740164 1102 24-25 -0.08056232041574418 1102 26-27 0.00938360562998497 1102 28-29 -0.15849457369339603 1102 30-31 -0.06436254661061014 1102 32-33 0.07288020503289516 1102 34-35 -0.02812762976718375 1102 36-37 -0.0338889498894801 1102 38-39 0.07919329634639638 1102 40-41 0.13078592607850226 1102 42-43 0.17348367269378429 1102 44-45 0.13740548481936798 1102 46-47 0.1823995800021052 1102 48-49 0.2893755333826249 1102 50-51 0.20081563074904096 1102 52-53 0.19065575927709943 1102 54-55 0.23933335469212835 1102 56-57 0.15589750880842246 1102 58-59 0.19040510387858234 1102 60-61 0.1532881815274436 1102 62-63 0.11675355844712954 1102 64-65 0.09164980304878867 1102 66-67 0.22187780385200995 1102 68-69 0.30476846168952676 1102 70-71 0.3271282306508212 1102 72-73 0.2258829673156839 1102 74-75 0.27713150124714403 1102 76-77 0.261325002851148 1102 78-79 0.28832659914143477 1102 80-81 0.18579064784038835 1102 82-83 0.23360638598468242 1102 84-85 0.1310076907088238 1102 86-87 0.13144522389942637 1102 88-89 0.21264521438231299 1102 90-91 0.12594619468258017 1102 92-93 0.3852456393892396 1102 94-95 0.3624502239715248 1102 96-97 0.3788605103338334 1102 98-99 0.5645617013899873 1102 100-101 1.126069692935527 1103 1 0.005124411342386281 1103 2 0.014405781228632009 1103 3 -0.0059962605855901074 1103 4 -0.02173604359395398 1103 5 0.021948250944724634 1103 6 -0.0481234895693845 1103 7 -0.023348026323361637 1103 8 -0.030995237591334046 1103 9 -0.06543469602587493 1103 10-11 -0.045486920869500125 1103 12-13 -0.1233644968622798 1103 14-15 -0.10788771763655092 1103 16-17 -0.21444663108464823 1103 18-19 -0.15421372727017513 1103 20-21 -0.08280856146745208 1103 22-23 -0.12126833486727406 1103 24-25 -0.08523815562612214 1103 26-27 -0.12278733094102989 1103 28-29 -0.25878340520258547 1103 30-31 -0.20911810298007083 1103 32-33 -0.2382322286599745 1103 34-35 -0.07259969582302261 1103 36-37 -0.2293062549561924 1103 38-39 -0.10265050471289072 1103 40-41 -0.0869774152106686 1103 42-43 -0.12486432094967626 1103 44-45 -0.23830591156244907 1103 46-47 -0.24744763128338576 1103 48-49 -0.3381238918029936 1103 50-51 -0.3026612332264804 1103 52-53 -0.21903759505476472 1103 54-55 -0.2171341209876303 1103 56-57 -0.26290665345811703 1103 58-59 -0.30859812729038794 1103 60-61 -0.25013155708970203 1103 62-63 -0.18248641485707395 1103 64-65 -0.3075834556095458 1103 66-67 -0.20621876699869546 1103 68-69 -0.2588957917741155 1103 70-71 -0.13331182282217213 1103 72-73 -0.051773997705076624 1103 74-75 0.05883810988299487 1103 76-77 0.14537858369039824 1103 78-79 0.1655119111588199 1103 80-81 3.9749515137188496E-4 1103 82-83 -0.013553878368888661 1103 84-85 0.052017766747098904 1103 86-87 0.033076425027484646 1103 88-89 0.17290366720582284 1103 90-91 0.04657730017780537 1103 92-93 0.14303335687763763 1103 94-95 0.0580170825142865 1103 96-97 0.10191138089784246 1103 98-99 0.36369535946800724 1103 100-101 0.9869396872179053 1104 1 -0.04538040794207632 1104 2 0.0016047158381553572 1104 3 -0.0834634351236545 1104 4 0.0036249703582669213 1104 5 -0.06418641229543454 1104 6 -0.09219038778959288 1104 7 -0.10959307427203413 1104 8 -0.11451017157415322 1104 9 -0.016426428872499343 1104 10-11 -0.12029138224298919 1104 12-13 -0.06289351532349485 1104 14-15 -0.09960937318336249 1104 16-17 -0.28107423072084003 1104 18-19 -0.04978572353439148 1104 20-21 -0.20811936818127919 1104 22-23 -0.23721806048242655 1104 24-25 -0.19755739430736696 1104 26-27 -0.33195465381172085 1104 28-29 -0.3083303700809168 1104 30-31 -0.25258093741521037 1104 32-33 -0.16932012337432667 1104 34-35 -0.1839158071070912 1104 36-37 -0.33294477911772447 1104 38-39 -0.30381162976198794 1104 40-41 -0.29388566669653926 1104 42-43 -0.2669925177824055 1104 44-45 -0.21275871879310415 1104 46-47 -0.27490748075323523 1104 48-49 -0.16423694609192552 1104 50-51 -0.3621975055399602 1104 52-53 -0.12133110443389938 1104 54-55 -0.09605252379720497 1104 56-57 -0.054077860649705656 1104 58-59 -0.12219752337921364 1104 60-61 -0.1277052503280487 1104 62-63 -0.08295908523103179 1104 64-65 -0.05223361238635249 1104 66-67 -0.05316324705110986 1104 68-69 0.05476846168952676 1104 70-71 -0.037198534538013917 1104 72-73 0.11915061920402081 1104 74-75 0.002295704859623271 1104 76-77 0.02015094702192144 1104 78-79 -0.08605763731176808 1104 80-81 -0.17422577252085958 1104 82-83 -0.14529344981170667 1104 84-85 -0.13233385280513588 1104 86-87 -0.169457895969213 1104 88-89 0.11083897464504133 1104 90-91 -0.017321457205760282 1104 92-93 0.055606887336693944 1104 94-95 0.13523347520305506 1104 96-97 0.2655600177229971 1104 98-99 0.3839377276625626 1104 100-101 1.0226214170734593 1105 1 -0.06372090320113699 1105 2 -0.01436824056478514 1105 3 -0.03670040486701964 1105 4 0.016170236694321716 1105 5 -0.07204694473995943 1105 6 -0.046054931188379555 1105 7 -0.08456271281029615 1105 8 -0.04463950002206474 1105 9 -0.07188480246365003 1105 10-11 -0.07561581023718844 1105 12-13 -0.09794919352027165 1105 14-15 -0.08126422284498602 1105 16-17 -0.16720616325459758 1105 18-19 -0.07519831873076299 1105 20-21 -0.042411389237415165 1105 22-23 -0.1268502678382788 1105 24-25 -0.2533219686710524 1105 26-27 -0.1335317257185551 1105 28-29 -0.2740331249913339 1105 30-31 -0.22458727315056848 1105 32-33 -0.19167743484879196 1105 34-35 -0.20814101225816017 1105 36-37 -0.16683844607197074 1105 38-39 -0.17198719728511236 1105 40-41 -0.09086541675671356 1105 42-43 -0.1733321496104523 1105 44-45 -0.23842682612061594 1105 46-47 -0.43103470303636726 1105 48-49 -0.31918350233321036 1105 50-51 -0.3134070158325102 1105 52-53 -0.2882807826817171 1105 54-55 -0.17977421143240946 1105 56-57 -0.3035276130339639 1105 58-59 -0.21425050990568906 1105 60-61 -0.29952871041057705 1105 62-63 -0.18634874157583425 1105 64-65 -0.2789901117391267 1105 66-67 -0.2241728441005435 1105 68-69 -0.10268537199241479 1105 70-71 -0.022581844113197747 1105 72-73 -0.08838031638416055 1105 74-75 -0.08565322581693025 1105 76-77 0.09553064632448738 1105 78-79 -0.07072113369700972 1105 80-81 -0.09899236339593998 1105 82-83 -0.27404096106837983 1105 84-85 -0.1583078160844913 1105 86-87 -0.06401319451841303 1105 88-89 0.19845549817663866 1105 90-91 0.2489795098783425 1105 92-93 0.40514550001271843 1105 94-95 0.4152489093771301 1105 96-97 0.45453847953307047 1105 98-99 0.5680236149103344 1105 100-101 0.876290722997684 1106 1 -0.20293726781001098 1106 2 -0.05435552592545889 1106 3 -0.043866158208487604 1106 4 -0.009316669980918846 1106 5 -0.22534552010584008 1106 6 -0.1537555127390604 1106 7 -0.15590073893731216 1106 8 -0.15144101140825228 1106 9 -0.3170001433179266 1106 10-11 -0.28446816814443565 1106 12-13 -0.23551568231207654 1106 14-15 -0.2535989220476651 1106 16-17 -0.08875636446942536 1106 18-19 -0.11325615939765754 1106 20-21 -0.14988510602447747 1106 22-23 -0.12584159500261904 1106 24-25 -0.17500684804337396 1106 26-27 -0.20256070988050823 1106 28-29 -0.17623380438200087 1106 30-31 -0.22113706859842353 1106 32-33 -0.2825120619252459 1106 34-35 -0.2277279761419777 1106 36-37 -0.1753567255544013 1106 38-39 -0.23801414422337785 1106 40-41 -0.2682232373814486 1106 42-43 -0.38054630319972915 1106 44-45 -0.31220921065529694 1106 46-47 -0.2205181534889462 1106 48-49 -0.1420594764246701 1106 50-51 -0.04305543198272943 1106 52-53 0.008867012964195453 1106 54-55 -0.018571751591032637 1106 56-57 -0.211927317380308 1106 58-59 -0.21000153713438863 1106 60-61 -0.08196531091869019 1106 62-63 0.08419669857919132 1106 64-65 0.12815828024744036 1106 66-67 0.05981095950605919 1106 68-69 0.17192886216666636 1106 70-71 -0.014613675209098176 1106 72-73 -0.03974445019570538 1106 74-75 0.0791594657711876 1106 76-77 0.1489372377671252 1106 78-79 0.19704074510796943 1106 80-81 0.20588625027798813 1106 82-83 0.03757584610915643 1106 84-85 -0.032813487065293856 1106 86-87 0.12963411794567392 1106 88-89 0.26844200118213735 1106 90-91 0.28758381378010967 1106 92-93 0.3966319620767891 1106 94-95 0.47836162888565426 1106 96-97 0.5281098652482115 1106 98-99 0.5852287723269427 1106 100-101 0.8747196297994861 1107 1 -0.04816894092709845 1107 2 -0.0260973226999468 1107 3 -0.05496507610131118 1107 4 -0.054117261724165644 1107 5 -0.05232592962008198 1107 6 -0.15032619342106557 1107 7 -0.1528785789811593 1107 8 -0.025621118034742096 1107 9 -0.15127079531963972 1107 10-11 -0.178633324446416 1107 12-13 -0.06135009310004591 1107 14-15 -0.15107947888193962 1107 16-17 -0.11720685812682774 1107 18-19 -0.15234700351813046 1107 20-21 -0.007341729650804041 1107 22-23 -0.07290472019143124 1107 24-25 -0.09882335688136124 1107 26-27 -0.17408455883894902 1107 28-29 -0.15603416567529393 1107 30-31 -0.008808872775304621 1107 32-33 -0.19104040848316828 1107 34-35 -0.06951680150214656 1107 36-37 -0.1484277614279037 1107 38-39 -0.23539139976401913 1107 40-41 -0.12245430796986057 1107 42-43 -0.14093413835280444 1107 44-45 -0.06328509474811028 1107 46-47 -0.24375571978062993 1107 48-49 -0.16817197593717736 1107 50-51 -0.041843190555518106 1107 52-53 -0.12407191816971164 1107 54-55 -0.11618149102210396 1107 56-57 -0.07397819886540447 1107 58-59 -0.11122685916254937 1107 60-61 -0.021787922295523288 1107 62-63 0.02154836391135717 1107 64-65 0.15299444152211095 1107 66-67 0.145786241462055 1107 68-69 0.2897621249966207 1107 70-71 0.23384459590118922 1107 72-73 0.3959698075287008 1107 74-75 0.2864360492003222 1107 76-77 0.33760391401255063 1107 78-79 0.32146095820047194 1107 80-81 0.36886717928428325 1107 82-83 0.19344380182768361 1107 84-85 0.2705415098082362 1107 86-87 0.3319481900785597 1107 88-89 0.41661722003029666 1107 90-91 0.5826263779809473 1107 92-93 0.6379986255028527 1107 94-95 0.5686368085293303 1107 96-97 0.6234722044253971 1107 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2316 16-17 0.2194088485084862 2316 18-19 0.20726874960597286 2316 20-21 0.27603920744986254 2316 22-23 0.36841706955902964 2316 24-25 0.40032680531916753 2316 26-27 0.5342529792733615 2316 28-29 0.5279469303943642 2316 30-31 0.4267568107856903 2316 32-33 0.19636543753914992 2316 34-35 0.16334017289386082 2316 36-37 0.15325388559063668 2316 38-39 0.18982060613665652 2316 40-41 -0.009106128459620777 2316 42-43 0.09052618799851331 2316 44-45 0.19481501036192128 2316 46-47 0.1673472211457252 2316 48-49 -0.20433685467513385 2316 50-51 -0.1430317162144945 2316 52-53 0.1919441510781823 2316 54-55 0.270923595191924 2316 56-57 0.15607839273509683 2316 58-59 0.09069635949465038 2316 60-61 0.11734481227728111 2316 62-63 0.12485921632305264 2316 64-65 -0.19255079830903554 2316 66-67 -0.10422396339478013 2316 68-69 -0.1258896308623818 2316 70-71 0.26348091748117497 2316 72-73 0.03509970741906443 2316 74-75 0.1983392209832111 2316 76-77 0.17283431133298066 2316 78-79 0.04403852361643956 2316 80-81 0.08102473547052469 2316 82-83 0.046820985808778914 2316 84-85 0.004431725958443877 2316 86-87 -0.07001885276936193 2316 88-89 0.05292037457832066 2316 90-91 0.20719394717271555 2316 92-93 0.37461410536356965 2316 94-95 0.3381527468148917 2316 96-97 0.5125748913641992 2316 98-99 0.33891875109732084 2316 100-101 -0.02331660147851977 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 4.0 4 14.0 5 35.0 6 57.0 7 201.0 8 512.0 9 939.0 10 1540.0 11 2617.0 12 3884.0 13 5633.0 14 7524.0 15 10155.0 16 14128.0 17 20267.0 18 18428.0 19 11261.0 20 9486.0 21 9590.0 22 10052.0 23 11162.0 24 12871.0 25 15690.0 26 18888.0 27 23423.0 28 29632.0 29 36828.0 30 46683.0 31 58613.0 32 75079.0 33 98865.0 34 135566.0 35 196465.0 36 300114.0 37 456288.0 38 372814.0 39 34817.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.47640525125021 20.07151033238881 15.585201022134035 21.866883394226942 2 15.627406860739008 20.888978205761685 39.97348459535961 23.5101303381397 3 19.076653225236072 25.161538615374933 30.436231085946297 25.325577073442695 4 12.896603091805442 17.497630636901384 37.38272079464268 32.22304547665049 5 13.440002653562749 37.91550939136831 34.2744213135721 14.370066641496843 6 31.432802521207204 37.173368114884475 17.25618241583822 14.137646948070099 7 28.193494165489096 33.424969843361076 20.620409740070013 17.76112625107981 8 26.51696551777961 35.43908700379342 19.43628913107419 18.607658347352775 9 26.402387361966 16.89298575845715 20.410081511943883 36.29454536763296 10-11 24.787378714653357 27.376124566123806 27.194647738380702 20.641848980842134 12-13 25.78531707317073 24.619536585365854 28.02248780487805 21.572658536585365 14-15 22.58933462235791 25.875957073920713 26.58840004702143 24.946308256699943 16-17 21.764606032108226 29.39115050808998 27.059980128079747 21.78426333172205 18-19 21.67931871604177 27.361327018933927 30.03286622451563 20.92648804050867 20-21 22.28720729280938 26.46023738018437 30.31467775930198 20.937877567704277 22-23 22.12234154897803 26.466394779214607 30.139509383141323 21.27175428866604 24-25 21.980490348461196 26.642768802800433 30.225302160099915 21.151438688638457 26-27 21.82076304369723 26.985220610418377 30.00702154533994 21.186994800544458 28-29 21.50205701095869 26.98372049235584 30.289693677557068 21.224528819128395 30-31 21.715517836381892 26.72461119987279 30.21503847454895 21.34483248919637 32-33 21.528035552884724 26.891063928172326 30.13666457086359 21.444235948079367 34-35 22.100243448095217 26.817507581230256 29.8726811825012 21.20956778817333 36-37 22.10921567298026 26.593254954449165 29.79522732006359 21.50230205250699 38-39 21.773722293058253 26.905743962054867 29.992371170830168 21.328162574056712 40-41 21.973600646984625 26.76318431159841 29.94654962670587 21.316665414711096 42-43 21.846754937714056 26.89595218796596 29.682946717593712 21.574346156726275 44-45 21.973004599227266 27.083956262221804 29.376448385880483 21.566590752670443 46-47 22.133265760349715 27.384837734601092 28.869817460267132 21.61207904478206 48-49 22.301011302215617 27.427981361172883 28.612272691133096 21.658734645478404 50-51 22.192091326936012 27.549339578143616 28.488231716150548 21.770337378769824 52-53 22.453904120179754 27.62516407504113 28.276293833217323 21.64463797156179 54-55 22.22757689881954 27.593702640175326 28.33875331747417 21.839967143530966 56-57 22.233113135807553 27.846833052944476 28.234296475977853 21.68575733527012 58-59 22.410321496842396 27.46919827971801 28.29200016194103 21.82848006149857 60-61 22.315254308387424 27.488489748693862 28.65419688224497 21.542059060673743 62-63 22.241917459828137 27.569338319686228 28.650562964574423 21.538181255911212 64-65 22.11166275674915 27.49876410185996 28.49762831939559 21.891944821995306 66-67 22.122168897664487 27.772301157634473 28.23063816959811 21.874891775102935 68-69 22.111047646270062 27.59992176102089 28.460989527976043 21.828041064733 70-71 22.056441326375072 27.619652226762316 28.40511499520274 21.91879145165987 72-73 22.25637996438273 27.565533681051292 28.1949330993318 21.983153255234182 74-75 22.1778970981826 27.812005976209303 28.00660348690253 22.003493438705565 76-77 22.174848509532815 27.71957276834775 28.159520693575868 21.94605802854357 78-79 22.190189007618542 27.775837520468222 28.01626141574506 22.017712056168172 80-81 22.10990676925861 28.12279815631069 27.984197088133744 21.783097986296955 82-83 22.197234555496074 27.98591303690389 27.899210900812026 21.91764150678801 84-85 22.192275414592295 27.79283518765885 27.85278300038071 22.16210639736815 86-87 22.17923847718851 27.664820116197564 28.049722724765125 22.1062186818488 88-89 22.241405296934975 27.61096984628772 27.938193157601305 22.209431699176 90-91 22.160993385236786 27.714760288005618 28.009205057659663 22.115041269097937 92-93 22.1554819700103 27.46805536687641 28.102545741719613 22.27391692139368 94-95 22.0689283454846 27.45271151229996 28.049259339290355 22.429100802925085 96-97 22.216626368389502 27.365399933370043 28.02557790265881 22.392395795581642 98-99 21.944569707839694 27.438333623153135 27.868356287630334 22.748740381376837 100-101 21.874447383062293 27.444338287789368 28.19215324803997 22.48906108110837 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 638.0 1 446.0 2 307.5 3 397.0 4 352.0 5 347.0 6 653.5 7 1541.5 8 2639.5 9 3111.5 10 2820.0 11 2456.0 12 2495.0 13 2680.5 14 2929.0 15 3124.5 16 3254.5 17 3461.0 18 3699.0 19 3968.0 20 4286.5 21 4817.5 22 5715.0 23 6831.0 24 8151.5 25 10096.0 26 12746.5 27 15770.0 28 18467.5 29 21486.0 30 25552.5 31 29462.0 32 33638.0 33 37985.0 34 43264.0 35 47465.5 36 52193.0 37 58218.0 38 61378.5 39 62438.5 40 63525.0 41 66854.5 42 70325.0 43 73073.0 44 75203.0 45 77430.5 46 77381.0 47 77043.0 48 81371.0 49 81242.0 50 76842.0 51 72650.5 52 67628.5 53 64683.0 54 62023.5 55 56771.0 56 50691.5 57 44986.0 58 40235.0 59 35886.5 60 30561.5 61 24399.5 62 18997.0 63 14962.0 64 11547.5 65 9027.0 66 6611.0 67 5095.5 68 3755.0 69 2234.5 70 1571.0 71 1209.0 72 934.0 73 712.0 74 557.5 75 450.0 76 285.5 77 153.0 78 93.5 79 61.0 80 41.5 81 22.5 82 11.0 83 8.0 84 7.0 85 5.5 86 2.5 87 1.0 88 0.5 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07189791471658612 2 0.0 3 0.0 4 0.0 5 0.002682758011812915 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.438870919829923E-4 12-13 0.006292286973161201 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.804386943455753E-4 24-25 7.560499851472761E-4 26-27 5.121628931642838E-4 28-29 0.0032680870325720967 30-31 3.6583063797448844E-4 32-33 0.0 34-35 0.0 36-37 9.755483679319692E-5 38-39 5.36551602362583E-4 40-41 1.4633225518979537E-4 42-43 0.013096736839486686 44-45 9.755483679319692E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 2.438870919829923E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.438870919829923E-5 82-83 3.1705321957788997E-4 84-85 6.097177299574807E-4 86-87 0.0 88-89 0.0 90-91 0.008243383709025139 92-93 0.00285347897620101 94-95 0.0 96-97 0.0 98-99 2.1949838278469304E-4 100-101 0.010901753011639755 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2050129.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.533386723158905 #Duplication Level Percentage of deduplicated Percentage of total 1 74.55800460242796 39.167844890864096 2 14.09451596939722 14.808653161921661 3 4.79479543012908 7.556605277682178 4 2.1592468655001187 4.537302024643457 5 1.171103229041245 3.076100941198193 6 0.7152815960469997 2.254569882065722 7 0.4766824227564709 1.7529219441158606 8 0.32315900090935934 1.3581309414272829 9 0.23500396435082596 1.1110998726645651 >10 1.303329229683176 12.575512541515218 >50 0.10663337593867245 3.897992068619 >100 0.05908691685086492 5.529050351539591 >500 0.0025073446509643756 0.8523702658815526 >1k 3.714584668095353E-4 0.28041576741570196 >5k 1.8572923340476764E-4 0.7175287242780348 >10k+ 9.286461670238382E-5 0.5239013441679233 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10739 0.5238206961610709 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8648 0.42182711429378345 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6060 0.29559115548338666 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2125 0.10365201409277172 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.438870919829923E-5 2.438870919829923E-5 16-17 0.0 0.0 0.0 4.877741839659846E-5 4.877741839659846E-5 18-19 0.0 0.0 0.0 4.877741839659846E-5 4.877741839659846E-5 20-21 0.0 0.0 0.0 4.877741839659846E-5 4.877741839659846E-5 22-23 0.0 0.0 0.0 4.877741839659846E-5 4.877741839659846E-5 24-25 0.0 0.0 0.0 1.2194354599149614E-4 4.877741839659846E-5 26-27 0.0 0.0 0.0 3.9021934717278767E-4 4.877741839659846E-5 28-29 0.0 0.0 0.0 9.267709495353706E-4 4.877741839659846E-5 30-31 0.0 0.0 0.0 0.0021705951186486313 4.877741839659846E-5 32-33 0.0 0.0 0.0 0.004292412818900664 4.877741839659846E-5 34-35 0.0 0.0 0.0 0.006243509554764603 4.877741839659846E-5 36-37 0.0 0.0 0.0 0.00914576594936221 4.877741839659846E-5 38-39 0.0 0.0 0.0 0.013877175533832262 4.877741839659846E-5 40-41 0.0 0.0 0.0 0.018730528664293807 4.877741839659846E-5 42-43 0.0 0.0 0.0 0.023510715667160457 4.877741839659846E-5 44-45 0.0 0.0 0.0 0.028047015578044114 4.877741839659846E-5 46-47 0.0 0.0 0.0 0.03448563480639511 4.877741839659846E-5 48-49 0.0 0.0 0.0 0.040973031453142704 4.877741839659846E-5 50-51 0.0 0.0 0.0 0.048362810340227366 4.877741839659846E-5 52-53 0.0 0.0 0.0 0.06487396646747595 4.877741839659846E-5 54-55 0.0 0.0 0.0 0.08760424344029083 4.877741839659846E-5 56-57 0.0 0.0 0.0 0.12206548953748765 4.877741839659846E-5 58-59 0.0 0.0 0.0 0.16586761125763305 4.877741839659846E-5 60-61 0.0 0.0 0.0 0.21588885382334477 4.877741839659846E-5 62-63 0.0 0.0 0.0 0.2503257112113433 4.877741839659846E-5 64-65 0.0 0.0 0.0 0.29183529426684857 4.877741839659846E-5 66-67 0.0 0.0 0.0 0.37087910077853636 4.877741839659846E-5 68-69 0.0 0.0 0.0 0.4738482310137557 4.877741839659846E-5 70-71 0.0 0.0 0.0 0.5819877675990145 4.877741839659846E-5 72-73 0.0 0.0 0.0 0.7099553247624906 4.877741839659846E-5 74-75 0.0 0.0 0.0 0.8433859527863856 4.877741839659846E-5 76-77 0.0 0.0 0.0 0.9674025390597372 4.877741839659846E-5 78-79 0.0 0.0 0.0 1.096491976846335 4.877741839659846E-5 80-81 0.0 0.0 0.0 1.2378245466504791 4.877741839659846E-5 82-83 0.0 0.0 0.0 1.40644808204752 9.755483679319692E-5 84-85 0.0 0.0 0.0 1.6405796903511924 9.755483679319692E-5 86-87 0.0 0.0 0.0 1.935341629721837 9.755483679319692E-5 88-89 0.0 0.0 0.0 2.2706132150708562 9.755483679319692E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6565 0.0 41.193214 1 GTATCAA 10480 0.0 35.01097 1 ATCAACG 13240 0.0 27.588085 3 TATCAAC 13430 0.0 27.339258 2 TCAACGC 13405 0.0 27.213078 4 CAACGCA 13625 0.0 26.808535 5 AACGCAG 13885 0.0 26.374311 6 GTACATG 9155 0.0 25.126682 1 GCGTATC 100 0.0016484536 23.763922 1 TACATGG 9490 0.0 23.223894 2 ACATGGG 9275 0.0 23.198908 3 CGCAGAG 15750 0.0 23.166435 8 ACGCAGA 15765 0.0 23.144396 7 GAGTACT 9250 0.0 21.879955 12-13 GCAGAGT 16775 0.0 21.467688 9 AGTACTT 9625 0.0 21.348333 12-13 GTAAGGT 615 0.0 20.85316 4 GTACTTT 9830 0.0 20.704748 14-15 AGAGTAC 14570 0.0 20.624317 10-11 TAAGGTG 740 0.0 19.256298 5 >>END_MODULE