FastQCFastQC Report
Thu 26 May 2016
SRR937375_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937375_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1383498
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50160.362559251983017No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37990.27459381943450584No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34290.2478500149620744No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27550.19913292249067216No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT24480.1769427928338169No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24030.1736901679655482No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT18770.1356705972831186No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC14890.10762574286337964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG51900.025.8293361
TACATGG50850.025.5111432
GTATCAA88350.024.0509641
ACATGGG52450.024.0072733
GTGTAGC7100.022.7641831
CCGACGT1050.002190686822.6292615
GAGTACT44800.021.57484812-13
GTAAGCG1551.0913936E-1121.44963394-95
AGAGTAC72150.020.87232810-11
TCAACGC100650.020.8208664
CAACGCA101000.020.7024155
ATCAACG101100.020.6796883
AGTACTT47050.020.64405812-13
TATCAAC103350.020.3681452
AACGCAG103250.020.2446656
GGTATCA68050.020.1185111
GTACTTT47300.020.03105214-15
TATCGTA1200.00475852819.795587
CATGGGG29900.019.7071784
TAGTAGT5600.018.6684674
Produced by FastQC (version 0.11.5)