FastQCFastQC Report
Thu 26 May 2016
SRR937375_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937375_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1383498
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55600.40187987261275404No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT47920.3463684081943017No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45930.33198457822129124No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33620.24300721793598543No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT16970.1226600978100438No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16180.11694993415241656No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG1109.481892E-525.9075265
GTATAGG3650.023.4359471
GTACATG50750.022.3802381
TACGTTG1100.002878668721.5896055
TACATGG52250.021.4532472
GAGTACT51300.021.01952612-13
ACATGGG51450.020.8636553
AGTACTT53900.020.57844412-13
GTACTTT52850.020.40084814-15
AGAGTAC81100.020.00250210-11
GGTATAG3506.366463E-1119.0091571
GTATCAA108200.018.3591211
CGCGTTA650.007568388718.26812670-71
GGTATCA89000.017.727641
ACGTATA1350.00940461417.591532
GTGTAAG5700.017.5084321
GGTGTGC12000.017.4175018
TACTTTT63350.017.3943714-15
ACTTTTT64250.016.965916-17
GTACCTG10650.016.5103021
Produced by FastQC (version 0.11.5)