##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937375_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1383498 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.24073833138899 33.0 31.0 34.0 31.0 34.0 2 32.45044011628495 34.0 31.0 34.0 31.0 34.0 3 32.52110592136743 34.0 31.0 34.0 31.0 34.0 4 36.13716319069489 37.0 35.0 37.0 35.0 37.0 5 36.0648580626788 37.0 35.0 37.0 35.0 37.0 6 36.10355634775041 37.0 35.0 37.0 35.0 37.0 7 36.04400512324557 37.0 35.0 37.0 35.0 37.0 8 36.01562705547821 37.0 35.0 37.0 35.0 37.0 9 37.71661831097696 39.0 37.0 39.0 35.0 39.0 10-11 37.63028352769574 39.0 37.0 39.0 35.0 39.0 12-13 37.5248370434941 39.0 37.0 39.0 35.0 39.0 14-15 38.75483303915148 40.0 38.0 41.0 35.0 41.0 16-17 38.71006499467292 40.0 38.0 41.0 35.0 41.0 18-19 38.680905935534426 40.0 38.0 41.0 34.0 41.0 20-21 38.56037558420756 40.0 38.0 41.0 34.0 41.0 22-23 38.507869545167395 40.0 38.0 41.0 34.0 41.0 24-25 38.54848760171681 40.0 38.0 41.0 34.0 41.0 26-27 38.38859109301206 40.0 38.0 41.0 34.0 41.0 28-29 38.2491254053132 40.0 38.0 41.0 34.0 41.0 30-31 38.32010129396645 40.0 38.0 41.0 34.0 41.0 32-33 38.23490745920847 40.0 38.0 41.0 34.0 41.0 34-35 38.09984112734533 40.0 38.0 41.0 34.0 41.0 36-37 37.94275886195716 40.0 37.0 41.0 33.0 41.0 38-39 37.831548003683416 40.0 37.0 41.0 33.0 41.0 40-41 37.66041150764222 40.0 37.0 41.0 33.0 41.0 42-43 37.46698224355944 40.0 37.0 41.0 33.0 41.0 44-45 37.178257576086125 40.0 36.5 41.0 31.5 41.0 46-47 36.861674176616084 39.5 36.0 41.0 31.0 41.0 48-49 36.55330763036882 39.0 36.0 41.0 30.5 41.0 50-51 36.30781902106111 39.0 35.5 40.5 30.0 41.0 52-53 36.07095059045983 39.0 35.0 40.0 29.5 41.0 54-55 35.85169367790918 39.0 35.0 40.0 29.0 41.0 56-57 35.539457230874206 38.0 35.0 40.0 28.0 41.0 58-59 35.26219915026982 38.0 34.0 40.0 28.0 41.0 60-61 34.889664097815825 38.0 34.0 40.0 27.0 41.0 62-63 34.505638244507765 37.0 34.0 40.0 26.0 41.0 64-65 34.084138538689615 37.0 33.0 39.5 26.0 41.0 66-67 33.62646892153079 36.0 33.0 39.0 25.5 40.0 68-69 33.14957087035904 36.0 32.0 39.0 24.5 40.0 70-71 32.692430708248224 35.0 32.0 38.0 23.5 40.0 72-73 32.19020374442175 35.0 31.0 37.5 22.0 39.5 74-75 31.692898363423726 35.0 31.0 37.0 20.5 39.0 76-77 30.397171878817318 33.5 29.5 35.5 20.0 38.0 78-79 31.04701958369293 34.0 31.0 36.0 20.0 38.0 80-81 30.84707097516585 34.0 31.0 35.5 19.5 37.0 82-83 30.426931589348158 34.0 30.0 35.0 18.0 37.0 84-85 29.97011018447443 34.0 30.0 35.0 14.5 36.5 86-87 29.570617015709455 34.0 29.5 35.0 7.5 36.0 88-89 29.09984401856743 34.0 29.0 35.0 4.5 35.5 90-91 28.507881832861344 33.5 29.0 35.0 2.0 35.0 92-93 27.96474985869152 33.0 27.0 35.0 2.0 35.0 94-95 27.41905662313932 33.0 26.5 35.0 2.0 35.0 96-97 26.697741160449816 32.5 25.5 34.0 2.0 35.0 98-99 25.667157451619012 32.0 24.0 34.0 2.0 35.0 100-101 22.670716546030427 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08073429551784983 1101 2 0.0939890238004395 1101 3 0.035587728737851876 1101 4 0.024949825122490665 1101 5 0.027727519130429812 1101 6 0.12054798916051368 1101 7 0.1023850991442572 1101 8 0.027542090565361832 1101 9 0.05118553970926598 1101 10-11 0.04978008042100868 1101 12-13 0.05094133444023896 1101 14-15 0.054986239437816664 1101 16-17 -0.0887421494817886 1101 18-19 0.039360992205516254 1101 20-21 0.06197019804918824 1101 22-23 -2.7608406901435956E-4 1101 24-25 0.14082816993158787 1101 26-27 0.04026480221863693 1101 28-29 -0.10706810065888561 1101 30-31 0.10649663707780377 1101 32-33 0.09801767010837636 1101 34-35 0.08681253180733961 1101 36-37 0.08908605616664289 1101 38-39 0.07333463008652785 1101 40-41 0.14241020351914102 1101 42-43 0.17578949059373628 1101 44-45 -0.01925052194207666 1101 46-47 0.2094686435768125 1101 48-49 0.3349444823876979 1101 50-51 0.25723469792498577 1101 52-53 0.09427333593131948 1101 54-55 0.18720682290300772 1101 56-57 0.23102988772201627 1101 58-59 0.21857352694419063 1101 60-61 0.28787815924518156 1101 62-63 0.342125865581977 1101 64-65 0.40354481222116334 1101 66-67 0.42497217484412175 1101 68-69 0.4743619784516184 1101 70-71 0.5551656253798498 1101 72-73 0.5195374883503945 1101 74-75 0.4415964918479709 1101 76-77 0.5887604743434203 1101 78-79 0.6349794544267446 1101 80-81 0.4729210723992985 1101 82-83 0.3274837740133769 1101 84-85 0.3849860171236905 1101 86-87 0.5289974022365236 1101 88-89 0.8265164803429457 1101 90-91 0.9849585859702401 1101 92-93 0.9629977063594097 1101 94-95 0.938554687449713 1101 96-97 1.071234274376902 1101 98-99 1.614451489642633 1101 100-101 2.4334316068775905 1102 1 0.08374173760503822 1102 2 0.07028912185688085 1102 3 0.05702864831457077 1102 4 0.051478627076818384 1102 5 -0.0014788471698921057 1102 6 0.05609231577348339 1102 7 -0.007556860510902652 1102 8 0.05117397647980937 1102 9 0.04811036499308585 1102 10-11 -0.024775831588918606 1102 12-13 0.003821954973140862 1102 14-15 0.10486140168438851 1102 16-17 0.13818001184088757 1102 18-19 0.20424247427479258 1102 20-21 0.1756544563060558 1102 22-23 0.042685248818436605 1102 24-25 -0.04405484249833336 1102 26-27 0.07251644452234274 1102 28-29 -0.06983811012444363 1102 30-31 -0.04904482719394565 1102 32-33 0.0589221982755177 1102 34-35 0.20249291881854958 1102 36-37 0.12758185334285344 1102 38-39 -0.00525108994816037 1102 40-41 -0.011865737510966312 1102 42-43 0.023085144936281665 1102 44-45 0.08676662943326363 1102 46-47 0.012151450825058419 1102 48-49 0.15337682627674099 1102 50-51 0.13445244557417624 1102 52-53 0.2221746446730819 1102 54-55 0.33097768823694196 1102 56-57 0.33999723436004103 1102 58-59 0.23802511936947468 1102 60-61 0.3672531486176709 1102 62-63 0.25622714711732897 1102 64-65 0.43854628590180056 1102 66-67 0.3616125514261981 1102 68-69 0.3292135051280738 1102 70-71 0.26914376956249697 1102 72-73 0.5191115379680866 1102 74-75 0.3397389457529414 1102 76-77 0.20875406950067443 1102 78-79 0.341028913415915 1102 80-81 0.48343167439783485 1102 82-83 0.5004926047611598 1102 84-85 0.6097320906150117 1102 86-87 0.7080133236553365 1102 88-89 0.7971274626644487 1102 90-91 0.9743913039474421 1102 92-93 1.1026426910274623 1102 94-95 1.0888346867695553 1102 96-97 1.3170449120838548 1102 98-99 1.550345673705344 1102 100-101 2.091693428913647 1103 1 0.028748970274449448 1103 2 -0.02019045976101097 1103 3 0.016266436230445436 1103 4 0.014983689945403 1103 5 -0.0048659618911557345 1103 6 0.0028042329872377536 1103 7 -0.08192984370885625 1103 8 -0.024915931164571248 1103 9 -0.00828108932982019 1103 10-11 -0.025777278122802727 1103 12-13 0.017369301139822824 1103 14-15 0.026277335811464297 1103 16-17 0.0019944104166143006 1103 18-19 -0.008973281817347356 1103 20-21 0.06911779223572267 1103 22-23 -0.005161084774528035 1103 24-25 0.11379204745381344 1103 26-27 -0.08580335073747136 1103 28-29 -0.030628140167841877 1103 30-31 0.05345656370401741 1103 32-33 0.11637240412841265 1103 34-35 0.22891497289666063 1103 36-37 0.17152643996796968 1103 38-39 0.2442031216908731 1103 40-41 0.18702710770991615 1103 42-43 0.32766898826552904 1103 44-45 0.34326323564223316 1103 46-47 0.4116451639662557 1103 48-49 0.3466031531933993 1103 50-51 0.4033430653583139 1103 52-53 0.4292465262798473 1103 54-55 0.4466903843535519 1103 56-57 0.5888138583725038 1103 58-59 0.48875061174735634 1103 60-61 0.5292955431876081 1103 62-63 0.47744334829680213 1103 64-65 0.539983361677919 1103 66-67 0.6491890508142077 1103 68-69 0.7184412785786094 1103 70-71 0.7373611947321805 1103 72-73 0.3943385325157749 1103 74-75 0.4529024040816374 1103 76-77 0.2655143899635668 1103 78-79 0.44285655334025265 1103 80-81 0.6638862198523796 1103 82-83 0.6880129118714287 1103 84-85 0.731363892106053 1103 86-87 0.7824269210738315 1103 88-89 0.9849932688289087 1103 90-91 1.2060937630550441 1103 92-93 1.1341632206814083 1103 94-95 1.1226685579167643 1103 96-97 1.4910896433623684 1103 98-99 1.881691506575045 1103 100-101 2.4112600251081524 1104 1 0.01021977056284129 1104 2 0.08330629582658844 1104 3 0.05716766189735267 1104 4 -0.0015483936883384786 1104 5 -0.003863798949552688 1104 6 -0.013799948699855236 1104 7 0.009750040934257242 1104 8 0.025848308200970394 1104 9 0.0434276345346305 1104 10-11 -0.07186595872843071 1104 12-13 -0.009505248247343445 1104 14-15 -0.02087479108110557 1104 16-17 0.025880495492316413 1104 18-19 -0.02888315925065399 1104 20-21 0.020354273850720972 1104 22-23 -0.018455965812741226 1104 24-25 -0.22637738297156318 1104 26-27 6.746593562567682E-4 1104 28-29 -0.16979901255428587 1104 30-31 -0.05763572180427445 1104 32-33 -0.01387993905832019 1104 34-35 -0.05134458009541021 1104 36-37 -0.0237980012721124 1104 38-39 0.02913102363752529 1104 40-41 0.010732947652236646 1104 42-43 0.026782182209295513 1104 44-45 -0.049638711005208336 1104 46-47 0.05478503418976288 1104 48-49 0.20295138679109215 1104 50-51 0.17309793197691903 1104 52-53 0.037671183814090625 1104 54-55 0.12171561867222636 1104 56-57 0.08576050581302042 1104 58-59 0.017658326239065048 1104 60-61 0.22263007815517 1104 62-63 0.18699273546334894 1104 64-65 -0.009908491242050843 1104 66-67 0.05678458073489878 1104 68-69 0.09443983661754629 1104 70-71 0.13263156243225893 1104 72-73 0.22247479783661106 1104 74-75 0.1536630385445079 1104 76-77 0.15504935751944515 1104 78-79 0.23820983380167604 1104 80-81 0.17196841163320187 1104 82-83 0.031120337970921952 1104 84-85 0.44307261597051095 1104 86-87 0.4974413406844995 1104 88-89 0.47306464590172936 1104 90-91 0.6872869858380284 1104 92-93 0.7339397167160158 1104 94-95 0.9311941527257055 1104 96-97 1.0806822893609258 1104 98-99 1.0617275556017205 1104 100-101 1.8853696141492478 1105 1 -0.004333079828974462 1105 2 -0.00976871763275966 1105 3 0.02338319099946773 1105 4 0.06297035967649833 1105 5 -0.013342174256131045 1105 6 -0.0010282193225776837 1105 7 -0.06033250976263815 1105 8 -0.022908346701228766 1105 9 0.035052627057211794 1105 10-11 -0.05383117377897406 1105 12-13 0.009799202311967292 1105 14-15 0.11882042935317827 1105 16-17 0.07166517575791431 1105 18-19 0.06440869933641125 1105 20-21 0.12210503654737437 1105 22-23 0.0798360423226896 1105 24-25 0.06084789668387458 1105 26-27 -0.00114235326562806 1105 28-29 0.05721316068253657 1105 30-31 0.09170581674929679 1105 32-33 0.13484172152671192 1105 34-35 0.04159539119130784 1105 36-37 0.026484495587375534 1105 38-39 0.05753683807791532 1105 40-41 0.012029980612695113 1105 42-43 -0.1685468241913739 1105 44-45 -0.11974354440832968 1105 46-47 -0.0011047785756801431 1105 48-49 0.11067750391737263 1105 50-51 0.11169516832315196 1105 52-53 0.03056748697853351 1105 54-55 0.07065786309406974 1105 56-57 0.11926203120633261 1105 58-59 0.22419418793673174 1105 60-61 0.16209260133516068 1105 62-63 0.28688715431840706 1105 64-65 0.28012470849474624 1105 66-67 0.19514687660137753 1105 68-69 0.3363118830373537 1105 70-71 0.34332591548603375 1105 72-73 0.30692012295475024 1105 74-75 0.2908419582071282 1105 76-77 0.19315114013594226 1105 78-79 0.22978771858961622 1105 80-81 0.2406766128654816 1105 82-83 0.13400004126696885 1105 84-85 0.25328931154986023 1105 86-87 0.39789118216323516 1105 88-89 0.5301581734485339 1105 90-91 0.6928628965875632 1105 92-93 0.7413339860227097 1105 94-95 1.032676027463996 1105 96-97 1.0254851846172492 1105 98-99 1.0457451723989948 1105 100-101 1.6432360076409047 1106 1 -0.1622040548116317 1106 2 0.008958581065357407 1106 3 -0.03345933848672189 1106 4 0.048650684534678135 1106 5 -0.006853172909359273 1106 6 -0.036743233810597076 1106 7 -0.11798616441716803 1106 8 -0.16737535074154408 1106 9 -0.21693947595058205 1106 10-11 -0.2696287291803472 1106 12-13 -0.39755015286903017 1106 14-15 -0.256868261827492 1106 16-17 -0.1489493918460525 1106 18-19 -0.037887448074101826 1106 20-21 0.035871973249008704 1106 22-23 0.042206721423205806 1106 24-25 -0.032930161104964384 1106 26-27 -0.05238352781568523 1106 28-29 -0.02411744169268104 1106 30-31 -0.054847666497650494 1106 32-33 -0.13193428549283936 1106 34-35 -0.5310309198726486 1106 36-37 -0.02056714094693035 1106 38-39 0.026047071518711107 1106 40-41 -0.03708135269342705 1106 42-43 -0.0839296344595084 1106 44-45 0.009091445184779445 1106 46-47 -0.12240242580255511 1106 48-49 -0.24158548428815152 1106 50-51 -0.04724965475973164 1106 52-53 -0.16133758586968838 1106 54-55 -0.22084343266563167 1106 56-57 -0.2634844692221847 1106 58-59 -0.24153959976273143 1106 60-61 -0.04896318775188746 1106 62-63 0.007399693599310808 1106 64-65 -0.09806877801711522 1106 66-67 -0.15789407756749796 1106 68-69 -0.010440300368756539 1106 70-71 0.19186869596189382 1106 72-73 0.22693629444395924 1106 74-75 0.0963173081642772 1106 76-77 -0.003718885983307274 1106 78-79 0.013119096380091122 1106 80-81 -0.03741050911958155 1106 82-83 0.1752921789643942 1106 84-85 0.2099017671675405 1106 86-87 0.2679249535381665 1106 88-89 0.3372774547610291 1106 90-91 0.40179777190895294 1106 92-93 0.3285465212224814 1106 94-95 -0.09203446716931651 1106 96-97 0.2674466281139658 1106 98-99 0.6856935970816878 1106 100-101 1.1108394643604882 1107 1 -0.010423292727267608 1107 2 -0.026326645923745673 1107 3 -0.03312135074440903 1107 4 0.014494856586040328 1107 5 -0.07253580415259364 1107 6 -0.10338817682066548 1107 7 -0.14525016230950882 1107 8 -0.05400698223437672 1107 9 -0.07891048180209737 1107 10-11 -0.14563125282216305 1107 12-13 0.0033641473056889026 1107 14-15 -0.081962551741249 1107 16-17 -0.02518816025338566 1107 18-19 0.022028592304167205 1107 20-21 -0.0562913909596503 1107 22-23 0.02728932909396775 1107 24-25 -0.1195134343515818 1107 26-27 -0.0865209830973086 1107 28-29 0.06597189106751244 1107 30-31 -0.07032057201361397 1107 32-33 -0.06768506893109105 1107 34-35 -0.12957635929137723 1107 36-37 -0.15961443149852528 1107 38-39 -0.19834021736894414 1107 40-41 -0.2753342853870677 1107 42-43 -0.18992120383192201 1107 44-45 -0.20741847605540897 1107 46-47 -0.15838841404405457 1107 48-49 0.05097219895320393 1107 50-51 0.04567790840064134 1107 52-53 0.03991418206619102 1107 54-55 -0.10365164282121242 1107 56-57 -0.08367325704214323 1107 58-59 -0.006658571448021178 1107 60-61 0.10280405482278354 1107 62-63 -0.04038501340864542 1107 64-65 0.11037848896200586 1107 66-67 0.12972551809516375 1107 68-69 0.14447438805854063 1107 70-71 0.1745656301530829 1107 72-73 0.24290404868007442 1107 74-75 0.20693566973836042 1107 76-77 0.13039101029778521 1107 78-79 0.13529369218141696 1107 80-81 0.06278829700371347 1107 82-83 0.12458483128420639 1107 84-85 0.2850074966664131 1107 86-87 0.388549832209236 1107 88-89 0.6888469311746839 1107 90-91 0.696603680281342 1107 92-93 0.6372477123259515 1107 94-95 0.4731550486242462 1107 96-97 0.6880543474073448 1107 98-99 0.8246916627518388 1107 100-101 1.6994796096778835 1108 1 0.02831773296500728 1108 2 0.07863282318610487 1108 3 0.025804258127500646 1108 4 0.07805230760099136 1108 5 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2316 14-15 -0.02186444721753844 2316 16-17 -0.10531723604648846 2316 18-19 -0.07152583667387091 2316 20-21 0.06470548595982706 2316 22-23 0.028124169176678038 2316 24-25 0.09326956778525641 2316 26-27 -0.10387224746909851 2316 28-29 -0.2297734524280841 2316 30-31 -0.21699072799461305 2316 32-33 -0.15908102536368318 2316 34-35 -0.10809285279000846 2316 36-37 -0.2693980651724317 2316 38-39 -0.1540084787387741 2316 40-41 -0.12794910834040962 2316 42-43 -0.31737014016779597 2316 44-45 -0.3868710285897734 2316 46-47 -0.27135698426145893 2316 48-49 -0.12428452518621924 2316 50-51 -0.5229757930121011 2316 52-53 -0.25003918800586433 2316 54-55 -0.2524902202191015 2316 56-57 0.03434631195543858 2316 58-59 -0.36773166537402346 2316 60-61 -0.1945075870870525 2316 62-63 -0.14078513106485957 2316 64-65 -0.0967881589604076 2316 66-67 -0.08313794013255205 2316 68-69 0.0036814197478634014 2316 70-71 0.1460737925532669 2316 72-73 0.22243965266307697 2316 74-75 -0.018998010219423378 2316 76-77 0.15161152054512428 2316 78-79 0.0012231330548395647 2316 80-81 0.14805531606229394 2316 82-83 0.25066904199264783 2316 84-85 0.24272955115162986 2316 86-87 0.46123542191777744 2316 88-89 0.5777928698723329 2316 90-91 0.7755912312152375 2316 92-93 1.0248107578917818 2316 94-95 1.0465729617835748 2316 96-97 1.0371438093896792 2316 98-99 0.9918833126207716 2316 100-101 1.9965953020760843 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 6.0 4 7.0 5 23.0 6 41.0 7 121.0 8 281.0 9 515.0 10 938.0 11 1573.0 12 2425.0 13 3501.0 14 4643.0 15 6424.0 16 8579.0 17 12103.0 18 10981.0 19 7402.0 20 6798.0 21 7083.0 22 7586.0 23 8490.0 24 9801.0 25 11834.0 26 14465.0 27 17845.0 28 22229.0 29 27427.0 30 34725.0 31 43271.0 32 56283.0 33 73381.0 34 100287.0 35 143549.0 36 215261.0 37 300733.0 38 207755.0 39 15131.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.62886612847709 19.859455582005456 15.509708527248378 22.001969762269074 2 15.672158543055357 20.847156989023475 39.61371827064441 23.866966197276756 3 19.634144754817136 24.420346108198203 30.0803470623015 25.865162074683155 4 13.069335842914121 17.198073289589143 37.32126826348863 32.411322604008106 5 13.544530065826708 37.78794215460856 34.408206545662715 14.25932123390202 6 31.819634000193712 37.10623361941976 17.029442760307568 14.044689620078959 7 28.586091197818863 32.78465165833272 20.53692885714327 18.092328286705147 8 26.369535770922692 35.532035463730345 19.236890837572588 18.86153792777438 9 25.923420200101482 17.141622177986523 20.3372899707842 36.59766765112779 10-11 25.00018070138157 26.99544921640653 27.26216445560456 20.742205626607337 12-13 26.066297096520262 24.008987942105335 28.027105505313322 21.89760945606108 14-15 22.502345503932787 25.844164574144667 26.45721208126069 25.196277840661857 16-17 21.849218430384433 29.148000213950436 27.05121366275918 21.95156769290595 18-19 21.840074940476963 27.7860539010537 29.302030071601116 21.071841086868215 20-21 22.653833963439734 26.593128352691892 29.438672233720865 21.314365450147505 22-23 22.370337924257523 26.359667811235422 29.425391789824364 21.844602474682695 24-25 22.390957400671923 26.612400758657778 29.499940729861162 21.496701110809138 26-27 22.198401342707324 26.739905028900086 29.636201922190775 21.425491706201818 28-29 21.796155277909293 27.35885879916586 29.473564373253918 21.371421549670934 30-31 22.186664141661467 26.676595623187787 29.372303704637996 21.76443653051275 32-33 22.11083371401146 26.79284941915264 29.298169275516518 21.798147591319385 34-35 22.525945104365892 26.758730406549198 29.175177701738637 21.540146787346277 36-37 22.420560058633985 26.627143660489573 29.4088968686619 21.543399412214544 38-39 22.06011288784869 27.009418885621 29.43663773755924 21.493830488971064 40-41 22.15724200540948 27.04915366700928 29.119087992899157 21.67451633468209 42-43 22.165781991960216 26.913279228782443 28.914875329900656 22.00606344935668 44-45 22.34596652832169 26.934010746672566 28.794692872703827 21.92532985230192 46-47 22.63649820961071 27.4860896076467 28.300293892726984 21.577118290015598 48-49 22.63772697900539 27.234733985882166 28.336036626001626 21.79150240911082 50-51 22.34712301716374 27.457394228253314 28.504161191414806 21.69132156316814 52-53 22.736787476382332 27.845866058353536 27.662923979651577 21.754422485612558 54-55 22.45977225843478 27.517423227211026 27.897474372930063 22.12533014142413 56-57 22.57675833286351 27.632891410034567 27.978717714084155 21.81163254301777 58-59 22.907478001413807 27.214639992251527 27.972031762966047 21.905850243368622 60-61 22.491756402972754 27.470910691594785 28.53867515529477 21.498657750137696 62-63 22.211163297670108 27.666935550322442 28.357178687645373 21.764722464362073 64-65 22.206356640920333 27.77568164175156 28.052371597212282 21.96559012011582 66-67 22.368879463504825 27.566863125208712 27.833759065788318 22.23049834549815 68-69 22.230534485774463 27.534878980670737 28.003835206122453 22.230751327432348 70-71 22.578673767508157 27.58457186060262 27.84246887238001 21.994285499509214 72-73 22.56529834564032 27.33625539936509 28.03520935369528 22.06323690129932 74-75 22.223450991616904 28.065635078619554 27.548323163459575 22.162590766303964 76-77 22.343183727045503 27.647998045533857 27.674850270835233 22.33396795658541 78-79 22.531727548576143 27.670766419611738 27.674597288901033 22.122908742911086 80-81 22.578167803639758 27.72150736755673 27.639505080600046 22.060819748203468 82-83 22.496544072338665 27.734881107053344 27.832279538920524 21.936295281687467 84-85 22.456889152311444 27.71183178863713 27.691340244561335 22.139938814490087 86-87 22.27379439652244 27.828518725722766 27.62508511035072 22.272601767404073 88-89 22.300502060718554 27.690860413242373 27.49967835154081 22.508959174498262 90-91 22.467464779263626 27.50560777592318 27.643642556784382 22.383284888028818 92-93 22.58281127911776 27.383324767957834 27.856733433226573 22.177130519697833 94-95 22.348930030979446 27.45457528670081 27.798919839421526 22.39757484289822 96-97 21.986117797062228 27.646805416415493 28.052335456935968 22.31474132958631 98-99 22.16980262660965 27.76397014950186 27.43520122414654 22.631025999741958 100-101 22.12792735676821 27.49061398813063 27.817583713249793 22.563874941851367 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 331.0 1 225.0 2 170.5 3 235.5 4 207.0 5 195.0 6 363.0 7 897.0 8 1477.5 9 1738.0 10 1653.0 11 1475.5 12 1488.5 13 1621.0 14 1820.5 15 1932.0 16 1974.5 17 2087.0 18 2279.5 19 2432.5 20 2577.5 21 2896.0 22 3432.0 23 4188.0 24 5178.0 25 6390.0 26 8163.0 27 10490.5 28 12345.5 29 14547.5 30 17366.5 31 19157.0 32 21101.5 33 23711.0 34 26528.5 35 29185.0 36 32350.5 37 35564.0 38 37185.0 39 38582.0 40 40304.5 41 42615.0 42 45216.0 43 47931.5 44 50570.5 45 52649.0 46 53773.0 47 56679.0 48 63004.0 49 61986.5 50 55097.0 51 50639.0 52 47789.5 53 46181.5 54 44577.5 55 41153.5 56 35827.5 57 31244.0 58 27887.0 59 24632.0 60 20554.0 61 16578.0 62 13093.5 63 9839.0 64 7346.0 65 5542.0 66 4078.5 67 3199.5 68 2442.5 69 1678.5 70 1175.0 71 778.5 72 591.5 73 477.0 74 355.5 75 267.5 76 161.0 77 82.0 78 52.5 79 33.5 80 18.5 81 10.0 82 5.0 83 2.0 84 0.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04264552605063397 2 0.0 3 0.0 4 0.0 5 0.0012287693946792838 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006432969183909193 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.0842082894228975E-4 22-23 5.421041447114488E-4 24-25 1.4456110525638634E-4 26-27 0.0017708735393907327 28-29 0.0031080637630123064 30-31 1.8070138157048294E-4 32-33 1.4456110525638634E-4 34-35 0.0 36-37 0.0 38-39 7.228055262819317E-5 40-41 0.0 42-43 0.009179630183780534 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.4456110525638634E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.9754303945506247E-4 84-85 3.6140276314096586E-5 86-87 0.0 88-89 0.0 90-91 0.01091436344685717 92-93 0.0015178916051920566 94-95 0.0 96-97 0.0 98-99 2.5298193419867615E-4 100-101 0.013697164723042608 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1383498.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.385074746970886 #Duplication Level Percentage of deduplicated Percentage of total 1 77.76828635109017 45.40507211552235 2 12.72561836771956 14.859723592014646 3 4.002545029645754 7.0106667220195265 4 1.8321128151071608 4.278721746196594 5 0.9575590200188789 2.795357747921922 6 0.5936614002630622 2.0796579137250175 7 0.4033808849422599 1.6485996183197176 8 0.2946450589982713 1.3762299034751775 9 0.2128973817372982 1.1187026591549896 >10 1.1141903234921862 11.604016601444505 >50 0.06578811568443835 2.6147763850614045 >100 0.026594046523339288 2.639927389208039 >500 0.0013606023888115125 0.583586841835676 >1k 0.0012369112625559204 1.5834333738508888 >5k 1.2369112625559205E-4 0.40152739024951845 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5560 0.40187987261275404 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4792 0.3463684081943017 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4593 0.33198457822129124 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3362 0.24300721793598543 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1697 0.1226600978100438 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1618 0.11694993415241656 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 7.228055262819317E-5 7 0.0 0.0 0.0 0.0 7.228055262819317E-5 8 0.0 0.0 0.0 0.0 7.228055262819317E-5 9 0.0 0.0 0.0 0.0 7.228055262819317E-5 10-11 0.0 0.0 0.0 0.0 7.228055262819317E-5 12-13 0.0 0.0 0.0 0.0 7.228055262819317E-5 14-15 0.0 0.0 0.0 3.6140276314096586E-5 1.0842082894228975E-4 16-17 0.0 0.0 0.0 2.1684165788457953E-4 1.4456110525638634E-4 18-19 0.0 0.0 0.0 4.3368331576915906E-4 1.4456110525638634E-4 20-21 0.0 0.0 0.0 5.782444210255454E-4 1.4456110525638634E-4 22-23 0.0 0.0 0.0 6.143846973396419E-4 1.4456110525638634E-4 24-25 0.0 0.0 0.0 7.228055262819317E-4 1.4456110525638634E-4 26-27 0.0 0.0 0.0 9.75787460480608E-4 1.4456110525638634E-4 28-29 0.0 0.0 0.0 0.0018070138157048293 1.4456110525638634E-4 30-31 0.0 0.0 0.0 0.003686308184037852 1.4456110525638634E-4 32-33 0.0 0.0 0.0 0.005710163657627261 1.4456110525638634E-4 34-35 0.0 0.0 0.0 0.00751717747333209 1.4456110525638634E-4 36-37 0.0 0.0 0.0 0.009251910736408728 1.4456110525638634E-4 38-39 0.0 0.0 0.0 0.010625241236344396 1.4456110525638634E-4 40-41 0.0 0.0 0.0 0.01250453560467742 1.4456110525638634E-4 42-43 0.0 0.0 0.0 0.014889793841407793 1.4456110525638634E-4 44-45 3.6140276314096586E-5 0.0 0.0 0.01745575345970865 1.4456110525638634E-4 46-47 7.228055262819317E-5 0.0 0.0 0.021467324130573374 1.4456110525638634E-4 48-49 7.228055262819317E-5 0.0 0.0 0.026129419775091832 1.4456110525638634E-4 50-51 7.228055262819317E-5 0.0 0.0 0.03209256536691777 1.4456110525638634E-4 52-53 7.228055262819317E-5 0.0 0.0 0.04998200214239558 1.4456110525638634E-4 54-55 7.228055262819317E-5 0.0 0.0 0.07152160682559715 1.4456110525638634E-4 56-57 7.228055262819317E-5 0.0 0.0 0.09710892245597752 1.4456110525638634E-4 58-59 7.228055262819317E-5 0.0 0.0 0.1323095515859076 1.4456110525638634E-4 60-61 7.228055262819317E-5 0.0 0.0 0.1832673411887838 1.4456110525638634E-4 62-63 7.228055262819317E-5 0.0 0.0 0.21803428700294472 1.4456110525638634E-4 64-65 7.228055262819317E-5 0.0 0.0 0.2550057896722655 1.4456110525638634E-4 66-67 7.228055262819317E-5 0.0 0.0 0.3279368672741124 1.4456110525638634E-4 68-69 7.228055262819317E-5 0.0 0.0 0.4237808800590966 1.4456110525638634E-4 70-71 1.0842082894228975E-4 0.0 0.0 0.5016270352396607 2.1684165788457953E-4 72-73 1.4456110525638634E-4 0.0 0.0 0.5774854752229494 2.1684165788457953E-4 74-75 1.4456110525638634E-4 0.0 0.0 0.7290577940842704 2.1684165788457953E-4 76-77 1.4456110525638634E-4 0.0 0.0 0.8420684381184504 2.1684165788457953E-4 78-79 1.4456110525638634E-4 0.0 0.0 0.9438033159426324 2.1684165788457953E-4 80-81 1.4456110525638634E-4 0.0 0.0 1.0743058537128352 2.1684165788457953E-4 82-83 1.4456110525638634E-4 0.0 0.0 1.2790043787558782 2.1684165788457953E-4 84-85 1.4456110525638634E-4 0.0 0.0 1.4857267592725107 2.1684165788457953E-4 86-87 1.4456110525638634E-4 0.0 0.0 1.764548991035766 2.1684165788457953E-4 88-89 1.4456110525638634E-4 0.0 0.0 2.1346615607684294 2.1684165788457953E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACCG 110 9.481892E-5 25.907526 5 GTATAGG 365 0.0 23.435947 1 GTACATG 5075 0.0 22.380238 1 TACGTTG 110 0.0028786687 21.589605 5 TACATGG 5225 0.0 21.453247 2 GAGTACT 5130 0.0 21.019526 12-13 ACATGGG 5145 0.0 20.863655 3 AGTACTT 5390 0.0 20.578444 12-13 GTACTTT 5285 0.0 20.400848 14-15 AGAGTAC 8110 0.0 20.002502 10-11 GGTATAG 350 6.366463E-11 19.009157 1 GTATCAA 10820 0.0 18.359121 1 CGCGTTA 65 0.0075683887 18.268126 70-71 GGTATCA 8900 0.0 17.72764 1 ACGTATA 135 0.009404614 17.59153 2 GTGTAAG 570 0.0 17.508432 1 GGTGTGC 1200 0.0 17.417501 8 TACTTTT 6335 0.0 17.39437 14-15 ACTTTTT 6425 0.0 16.9659 16-17 GTACCTG 1065 0.0 16.510302 1 >>END_MODULE