FastQCFastQC Report
Thu 26 May 2016
SRR937374_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937374_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1334016
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48970.367087051429668No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36840.2761586067933218No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32650.24474968815966225No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27050.2027711811552485No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT24660.18485535405872192No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19520.14632508155824217No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT17230.129158870658223No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC15390.11536593264248704No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG48000.024.679561
TACATGG47400.024.3732282
GTATCAA87850.022.8533541
GAGTACT42800.022.02728712-13
ACATGGG50000.021.8697073
GTGTAGG5600.021.2388631
GTACTTT46300.020.31163214-15
AGAGTAC70200.020.29836510-11
AGTACTT46550.020.04874212-13
TCAACGC100550.019.9540864
CAACGCA101350.019.843495
ATCAACG100900.019.7898853
TATCAAC102150.019.6408022
CATGGGG28350.019.6215954
AACGCAG103550.019.4509896
CGGTACT1250.00599900219.017854
GGTATCA68300.018.5284981
GTACCTG10800.018.06091
CGCAGAG111650.017.9527388
ACGCAGA112200.017.9084137
Produced by FastQC (version 0.11.5)