##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937374_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1334016 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.526136867923622 31.0 31.0 34.0 30.0 34.0 2 31.641715691565917 33.0 31.0 34.0 30.0 34.0 3 31.675213790539242 33.0 31.0 34.0 30.0 34.0 4 35.2947280992132 37.0 35.0 37.0 33.0 37.0 5 35.28393512521589 37.0 35.0 37.0 33.0 37.0 6 35.320403953175976 37.0 35.0 37.0 33.0 37.0 7 35.255140118259455 37.0 35.0 37.0 33.0 37.0 8 35.24785834652658 37.0 35.0 37.0 33.0 37.0 9 36.84028002662637 39.0 37.0 39.0 33.0 39.0 10-11 36.708180786437346 39.0 37.0 39.0 32.0 39.0 12-13 36.56120878610152 39.0 37.0 39.0 32.0 39.0 14-15 37.70201181994818 40.0 37.5 41.0 32.0 41.0 16-17 37.659705355857795 40.0 37.0 41.0 32.0 41.0 18-19 37.62000868055556 40.0 37.0 41.0 32.0 41.0 20-21 37.54711825045577 40.0 37.0 41.0 32.0 41.0 22-23 37.388385146804836 40.0 37.0 41.0 32.0 41.0 24-25 37.21259377698618 40.0 37.0 41.0 31.0 41.0 26-27 37.04530417926022 39.0 36.5 41.0 31.0 41.0 28-29 37.10179900391 39.0 37.0 41.0 31.0 41.0 30-31 36.95255191841777 39.0 36.5 41.0 31.0 41.0 32-33 36.75676641059778 39.0 36.0 41.0 30.0 41.0 34-35 36.6285201976588 39.0 36.0 41.0 30.0 41.0 36-37 36.635426411677216 39.0 36.0 41.0 30.0 41.0 38-39 36.544860781279986 39.0 36.0 41.0 30.0 41.0 40-41 36.380013433122244 39.0 35.5 41.0 30.0 41.0 42-43 36.14093346706486 39.0 35.0 41.0 29.5 41.0 44-45 35.91997997025523 39.0 35.0 41.0 28.5 41.0 46-47 35.594552838946456 39.0 35.0 40.5 27.0 41.0 48-49 35.2846191499952 39.0 35.0 40.0 26.0 41.0 50-51 34.42250955011035 38.0 34.0 39.5 25.0 40.5 52-53 34.52141803396661 38.0 34.0 39.5 24.5 40.5 54-55 34.834528596358666 38.0 34.0 40.0 25.0 41.0 56-57 34.61240494866628 38.0 34.0 40.0 24.0 41.0 58-59 34.35463442717329 38.0 34.0 40.0 23.0 41.0 60-61 34.04478844331702 38.0 34.0 40.0 22.5 41.0 62-63 33.70634047867492 37.0 33.0 40.0 22.0 41.0 64-65 33.33319877722606 37.0 33.0 40.0 21.0 41.0 66-67 32.93400941218097 36.0 32.0 39.0 19.5 41.0 68-69 32.443624364325466 36.0 32.0 39.0 18.0 40.5 70-71 31.985711565678372 35.0 31.0 38.5 15.5 40.0 72-73 31.581431182234695 35.0 31.0 38.0 12.0 40.0 74-75 31.119790167434275 35.0 31.0 37.0 8.0 39.0 76-77 30.669437248128958 35.0 30.0 36.5 7.0 39.0 78-79 30.16700736722798 34.0 30.0 36.0 3.5 38.5 80-81 29.654500395797353 34.0 29.0 36.0 2.0 37.5 82-83 29.152406717760506 34.0 29.0 35.0 2.0 37.0 84-85 28.693821513385146 34.0 29.0 35.0 2.0 36.5 86-87 28.248436675422184 34.0 27.5 35.0 2.0 36.0 88-89 27.821303117803684 33.5 27.0 35.0 2.0 36.0 90-91 27.368439359048168 33.0 26.0 35.0 2.0 35.0 92-93 26.884201913620224 33.0 25.5 35.0 2.0 35.0 94-95 26.30912035537805 32.5 24.5 35.0 2.0 35.0 96-97 25.63250590697563 32.0 22.5 34.5 2.0 35.0 98-99 24.815385647548453 32.0 17.5 34.0 2.0 35.0 100-101 22.88353175674055 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.11972164159013232 1101 2 -0.10375742024262635 1101 3 -0.07138346171112175 1101 4 -0.08122847925075405 1101 5 -0.0799295755308691 1101 6 -0.2229259156023744 1101 7 -0.28222859837147496 1101 8 -0.11464013398013151 1101 9 -0.0980386413915113 1101 10-11 -0.19783407476055004 1101 12-13 -0.2396724023408794 1101 14-15 -0.2528243050952099 1101 16-17 0.003948958121164026 1101 18-19 -0.05950549308306563 1101 20-21 -0.07946290788350296 1101 22-23 -0.15324382290990712 1101 24-25 -0.1400660363373305 1101 26-27 -0.18851098521916043 1101 28-29 -0.2037738045155777 1101 30-31 -0.14929367965088858 1101 32-33 -0.1339829270748183 1101 34-35 -0.3319377254166085 1101 36-37 -0.24436333561268242 1101 38-39 -0.2970062383448422 1101 40-41 -0.343386411563408 1101 42-43 -0.11424398079238074 1101 44-45 -0.19169087672856477 1101 46-47 0.014352089782683208 1101 48-49 -0.053788934808935096 1101 50-51 -0.1941358859478015 1101 52-53 -0.15682369258487228 1101 54-55 0.004393919119493717 1101 56-57 0.07800236943526784 1101 58-59 0.009309762611870553 1101 60-61 -0.3024739721385572 1101 62-63 0.00951863328282343 1101 64-65 -0.10996598730142892 1101 66-67 -0.12624810705849399 1101 68-69 -0.06469565530025534 1101 70-71 0.10033556197595317 1101 72-73 0.07885330400126733 1101 74-75 0.01137693278088392 1101 76-77 0.08915651705360261 1101 78-79 0.11487769670563353 1101 80-81 -0.001024480304444353 1101 82-83 0.1041419638077592 1101 84-85 0.11238220454874437 1101 86-87 0.15000993281844188 1101 88-89 0.34686054430736846 1101 90-91 0.4972202751143868 1101 92-93 0.6444675807929947 1101 94-95 0.5325146418662889 1101 96-97 0.5287064912707962 1101 98-99 0.7221642539427364 1101 100-101 1.033488168205654 1102 1 0.07797908026186562 1102 2 0.052474091350664054 1102 3 0.12254596163093012 1102 4 -0.05587429588452153 1102 5 -0.06078588181705413 1102 6 -0.13590665455186013 1102 7 0.08561256942560647 1102 8 -0.006402709326700062 1102 9 0.10584888341895748 1102 10-11 0.12368655177402843 1102 12-13 0.16130093236341025 1102 14-15 0.22617717155482353 1102 16-17 0.26319390168492873 1102 18-19 0.33227336686558573 1102 20-21 0.2660126374891618 1102 22-23 0.31121429282435287 1102 24-25 0.2023532525988827 1102 26-27 0.21634454478945742 1102 28-29 0.18669824976140603 1102 30-31 0.19307581339464264 1102 32-33 0.3037438124571423 1102 34-35 0.21127662893525212 1102 36-37 0.25506347331052837 1102 38-39 0.38270874612691586 1102 40-41 0.4315655017562037 1102 42-43 0.5635938331841714 1102 44-45 0.4877657074456039 1102 46-47 0.5899800697975692 1102 48-49 0.41250382303822875 1102 50-51 0.4363137359533411 1102 52-53 0.43909072954323847 1102 54-55 0.503748860459929 1102 56-57 0.5652754818614767 1102 58-59 0.40676898785241633 1102 60-61 0.5222627960166193 1102 62-63 0.46740813270223214 1102 64-65 0.3969277827212281 1102 66-67 0.4924149621018046 1102 68-69 0.40304199087759685 1102 70-71 0.4010166082602922 1102 72-73 0.49275001817231967 1102 74-75 0.6230719418329684 1102 76-77 0.6828161274182563 1102 78-79 0.5467344428106529 1102 80-81 0.6019937966716071 1102 82-83 0.6415415039628947 1102 84-85 0.8118840572250434 1102 86-87 0.8124970195327386 1102 88-89 0.7340647769042405 1102 90-91 0.7152797104128368 1102 92-93 0.963094527745028 1102 94-95 0.8305616201170594 1102 96-97 1.0117823484464914 1102 98-99 1.2222581932175913 1102 100-101 1.4908487545159268 1103 1 0.06177547630112912 1103 2 -0.01620163405200259 1103 3 -0.041126919900278835 1103 4 -0.09300997871529404 1103 5 -0.014745865098532818 1103 6 0.006464660264711597 1103 7 0.02612610366840329 1103 8 0.03677350729012829 1103 9 0.10017406719337885 1103 10-11 0.15341039461996786 1103 12-13 0.15176319074206646 1103 14-15 0.16346334258353323 1103 16-17 -0.06854908989549102 1103 18-19 0.06836074912499868 1103 20-21 -0.17943512145395601 1103 22-23 -0.13298146248205 1103 24-25 -0.07702884092140039 1103 26-27 0.11950371972202589 1103 28-29 -0.002900451968180562 1103 30-31 -0.0974867884030246 1103 32-33 -0.10138560523863305 1103 34-35 -0.057772731624552875 1103 36-37 0.12255979997656397 1103 38-39 -0.08563190873912419 1103 40-41 -0.1137931707590667 1103 42-43 -0.15170339447205095 1103 44-45 -0.20064320033539929 1103 46-47 -0.17776800412584493 1103 48-49 -0.1433292373983477 1103 50-51 -0.03161407280060757 1103 52-53 -0.20753965980862432 1103 54-55 -0.2550052006746881 1103 56-57 -0.20058098665342072 1103 58-59 -0.1692372471203072 1103 60-61 -0.26027233225921975 1103 62-63 -0.38114763303170207 1103 64-65 -0.1842585533381964 1103 66-67 -0.08504552532053111 1103 68-69 -0.029405289778040355 1103 70-71 0.04405310891954883 1103 72-73 0.06005039778606758 1103 74-75 0.07907568841817181 1103 76-77 0.14090834210469438 1103 78-79 0.0527528935039534 1103 80-81 0.08011376064923326 1103 82-83 0.0608549998565131 1103 84-85 -0.15866921581246984 1103 86-87 -0.059275347947160384 1103 88-89 0.04628577587872584 1103 90-91 0.20474903753561335 1103 92-93 0.18625549086949533 1103 94-95 0.09977633456650992 1103 96-97 0.19659305863875431 1103 98-99 0.15960118781423205 1103 100-101 0.27790741582807144 1104 1 0.01514629185158256 1104 2 -0.019784498319289412 1104 3 0.05752741323638588 1104 4 0.0471916909732073 1104 5 0.03915429166355722 1104 6 0.033875954025667454 1104 7 0.05260446920830475 1104 8 -0.017994708765925793 1104 9 0.1475666054902689 1104 10-11 0.1571254192324858 1104 12-13 0.13382814364883444 1104 14-15 0.28615013279365087 1104 16-17 0.2225982664669388 1104 18-19 0.16685344827529747 1104 20-21 0.18430379430365207 1104 22-23 0.18344337677221034 1104 24-25 0.06301900058523557 1104 26-27 -0.009148589288777487 1104 28-29 -0.020326910523131403 1104 30-31 0.14740592754320403 1104 32-33 -0.1376559487801643 1104 34-35 0.14049672151692505 1104 36-37 0.33538519435810343 1104 38-39 0.1271587632706641 1104 40-41 0.20240058976531117 1104 42-43 0.12109439966143043 1104 44-45 0.2395386318430326 1104 46-47 0.2035125730945495 1104 48-49 0.019530260273903366 1104 50-51 -0.0674760920168822 1104 52-53 -0.05955950593199333 1104 54-55 0.15679593757310784 1104 56-57 0.24914734443326836 1104 58-59 0.2435431158442185 1104 60-61 0.35888233141289305 1104 62-63 0.17265670075095585 1104 64-65 0.2642146582174121 1104 66-67 0.35151508648228 1104 68-69 0.3317601329843285 1104 70-71 0.4334095983962527 1104 72-73 0.6073870842059073 1104 74-75 0.5739961284286785 1104 76-77 0.6399369231924688 1104 78-79 0.48802770088899905 1104 80-81 0.5753201891868258 1104 82-83 0.6836291343273793 1104 84-85 0.9057121176558063 1104 86-87 0.651482854069215 1104 88-89 0.5045314509246985 1104 90-91 0.6493632208438953 1104 92-93 0.8199366221405135 1104 94-95 0.8766706956533348 1104 96-97 0.9450749642455811 1104 98-99 1.088918955117279 1104 100-101 1.189570472085851 1105 1 -0.05178069308694333 1105 2 -0.014454903081428938 1105 3 -0.1477788657246073 1105 4 0.029641276547458517 1105 5 -0.13866574881687654 1105 6 -0.2028651608575629 1105 7 -0.09035492816425972 1105 8 0.09679834523092268 1105 9 0.05002815821384843 1105 10-11 0.021983716666710507 1105 12-13 -0.15358977533131934 1105 14-15 -0.01886384994949708 1105 16-17 -0.1446818336525908 1105 18-19 -0.18914339981353834 1105 20-21 -0.07422778590784418 1105 22-23 -0.00893641447688509 1105 24-25 0.047955983430917115 1105 26-27 -0.012526921163676263 1105 28-29 -0.0643956570204196 1105 30-31 -0.1719634373972596 1105 32-33 -0.35991821756626763 1105 34-35 -0.25984010036216887 1105 36-37 -0.17224386334125796 1105 38-39 -0.09910997227078866 1105 40-41 -0.13643969233354625 1105 42-43 -0.29659268185704946 1105 44-45 -0.18398159221796107 1105 46-47 -0.2133833000648977 1105 48-49 -0.10834533193845886 1105 50-51 -0.2808405324473071 1105 52-53 -0.2359402579240566 1105 54-55 -0.32478617013404687 1105 56-57 -0.28246390861996673 1105 58-59 -0.28351767081325363 1105 60-61 -0.30673557099304105 1105 62-63 -0.32959247720982177 1105 64-65 -0.20300827488594564 1105 66-67 -0.29687222777759104 1105 68-69 -0.3315872416169441 1105 70-71 -0.18966550951175876 1105 72-73 -0.24863940083726277 1105 74-75 -0.18548945270143236 1105 76-77 -0.2827002319970191 1105 78-79 -0.22767581158169747 1105 80-81 -0.07899858926500514 1105 82-83 -0.027336257856610757 1105 84-85 -0.033685226816903224 1105 86-87 -0.09605847262694311 1105 88-89 -0.16195112015834212 1105 90-91 -0.04045838154616277 1105 92-93 -0.028624440519873673 1105 94-95 0.055117466419218886 1105 96-97 0.11594549008380994 1105 98-99 0.432130898669417 1105 100-101 0.6690916175611612 1106 1 -0.18727610335897538 1106 2 -0.09519934583786593 1106 3 -0.14078518844489807 1106 4 -0.11768421433652065 1106 5 -0.143692914430801 1106 6 -0.2824067675520965 1106 7 -0.13142332223715414 1106 8 -0.3519725849779647 1106 9 -0.2126245651604819 1106 10-11 -0.6652906536846359 1106 12-13 -0.7144134987667243 1106 14-15 -0.7583424017223521 1106 16-17 -0.6932873739029617 1106 18-19 -0.3543377408457573 1106 20-21 -0.376952961360594 1106 22-23 -0.30650771931907883 1106 24-25 -0.34713039993235384 1106 26-27 -0.4840616804633413 1106 28-29 -0.06407881621921518 1106 30-31 -0.09240898792185703 1106 32-33 -0.02624460274632412 1106 34-35 -0.15081021583756637 1106 36-37 -0.12469503336557608 1106 38-39 -0.3335131196918155 1106 40-41 -0.41347178832237574 1106 42-43 -0.36678771984504976 1106 44-45 -0.1455821472766914 1106 46-47 -0.06758422067302661 1106 48-49 -0.15117741571097554 1106 50-51 -0.15391407616210273 1106 52-53 -0.36728290801622876 1106 54-55 -0.4020478699857222 1106 56-57 -0.40187765133521225 1106 58-59 -0.3501175132542613 1106 60-61 -0.5036866634908179 1106 62-63 -0.5469930357141735 1106 64-65 -0.4288899239616626 1106 66-67 -0.372366165828403 1106 68-69 -0.5138043754528177 1106 70-71 -0.4493280576134566 1106 72-73 -0.21569876424254275 1106 74-75 -0.4848491930893104 1106 76-77 -0.3641431967373201 1106 78-79 -0.16525112587743607 1106 80-81 -0.4112132955020513 1106 82-83 -0.3982226133416553 1106 84-85 -0.6221899982232344 1106 86-87 -0.46421526731942464 1106 88-89 -0.13957429730571036 1106 90-91 -0.21396638714060146 1106 92-93 -0.03147600773068504 1106 94-95 0.06140807975597795 1106 96-97 0.061323106996187704 1106 98-99 0.463377071285791 1106 100-101 0.5740246549884667 1107 1 0.05876164490380731 1107 2 0.07397130615835934 1107 3 0.08967545153743117 1107 4 0.14485442632454948 1107 5 -0.05375588414833743 1107 6 -0.04693983529749346 1107 7 -0.0015880156244136856 1107 8 0.10094782491096055 1107 9 0.11882998194193561 1107 10-11 0.1337166476338041 1107 12-13 0.0340292100019397 1107 14-15 0.09591890498237632 1107 16-17 0.19003704719624182 1107 18-19 0.07949118655428578 1107 20-21 -0.018237215977251253 1107 22-23 0.0685664234196608 1107 24-25 0.14401375973459807 1107 26-27 0.24718662646393597 1107 28-29 0.2803434511404177 1107 30-31 0.24493921457618484 1107 32-33 0.10739980280430217 1107 34-35 0.3167544549250394 1107 36-37 0.22491294581974586 1107 38-39 0.21886010251888166 1107 40-41 0.2212917655744917 1107 42-43 0.27199892731204045 1107 44-45 0.35057954985438045 1107 46-47 0.45625424332104814 1107 48-49 0.5059422509211089 1107 50-51 0.3017043405753128 1107 52-53 0.32593818823220744 1107 54-55 0.3166299908498189 1107 56-57 0.23715907546134218 1107 58-59 0.2104049484290087 1107 60-61 0.3617023479762622 1107 62-63 0.348364579848635 1107 64-65 0.5716839164996159 1107 66-67 0.4882803957779629 1107 68-69 0.5758949952528241 1107 70-71 0.533990791241429 1107 72-73 0.5384721109791322 1107 74-75 0.46536129340454124 1107 76-77 0.5224065803561224 1107 78-79 0.7359836968899458 1107 80-81 0.6571087976745993 1107 82-83 0.8294257216482457 1107 84-85 0.7700117465605913 1107 86-87 0.7757646793145838 1107 88-89 0.6247190967986569 1107 90-91 0.7440665569076899 1107 92-93 1.0053498136466565 1107 94-95 1.0365812137506616 1107 96-97 1.2816523326455602 1107 98-99 1.2700257322184143 1107 100-101 1.3646878561219822 1108 1 -0.1680156012595866 1108 2 -0.10965485510526207 1108 3 -0.2367435367880084 1108 4 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-1.0688531410543831 2316 60-61 -1.2085167322081602 2316 62-63 -1.4009372721395223 2316 64-65 -1.5353496459065639 2316 66-67 -1.3818980892185486 2316 68-69 -1.723715893179559 2316 70-71 -1.8605804945776896 2316 72-73 -1.4279866726373633 2316 74-75 -1.4684718960612315 2316 76-77 -1.7307551602406264 2316 78-79 -1.5304222237620735 2316 80-81 -1.7468211287856548 2316 82-83 -1.792102776396714 2316 84-85 -1.8260834201584935 2316 86-87 -2.398589979875428 2316 88-89 -2.28671982417233 2316 90-91 -2.3267263206442905 2316 92-93 -2.8659996863856847 2316 94-95 -2.4496359392775844 2316 96-97 -2.3626222960311445 2316 98-99 -2.8169366324735847 2316 100-101 -3.529935846560644 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6224.0 3 1434.0 4 1359.0 5 1396.0 6 1675.0 7 1868.0 8 2311.0 9 2867.0 10 3382.0 11 4302.0 12 5439.0 13 6603.0 14 7453.0 15 8521.0 16 10427.0 17 12991.0 18 11239.0 19 8808.0 20 9070.0 21 9485.0 22 10304.0 23 11733.0 24 13040.0 25 14844.0 26 17151.0 27 19837.0 28 23587.0 29 27896.0 30 34085.0 31 41985.0 32 52513.0 33 67482.0 34 91037.0 35 126725.0 36 183726.0 37 254169.0 38 201455.0 39 25583.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.926439120110516 19.004932750271617 15.107563450096157 22.961064679521712 2 14.775796033687561 19.835547911673558 40.87944416577866 24.50921188886023 3 19.118196659304896 23.995388929369856 29.957671695079796 26.928742716245452 4 12.275092008669194 16.385589645095454 37.773713570658295 33.56560477557706 5 13.382104552297957 37.40060131784567 34.59111740136838 14.626176728487996 6 31.618065834654484 36.33731807560241 17.646121826419517 14.398494263323592 7 28.159500814346927 32.383286956626264 20.930894699833523 18.526317529193285 8 25.98001687423884 34.92772100857761 19.775015157514183 19.31724695966937 9 25.65323645127387 16.877882477914934 20.5775210483336 36.8913600224776 10-11 24.69073971865721 26.4931941134501 27.716408932545285 21.0996572353474 12-13 25.644824648267218 23.745287314178555 28.22382271135147 22.386065326202758 14-15 22.36289661871825 25.411358038418584 26.612854452711527 25.612890890151636 16-17 21.70804655904664 28.723608063162594 27.168058684894003 22.400286692896763 18-19 21.680983627617028 27.502275350674456 29.31752251028932 21.499218511419198 20-21 22.39477151480643 26.344154273515187 29.344839345996153 21.916234865682224 22-23 22.039266856420415 26.17666479617247 29.359847776327957 22.424220571079154 24-25 22.048147206472805 26.36405254949001 29.441595007190045 22.146205236847134 26-27 21.887966337472548 26.43198691235544 29.61669215492454 22.063354595247468 28-29 21.58345591936519 26.98668257405123 29.50997642441463 21.919885082168953 30-31 21.920572919112594 26.474215897363262 29.312700691315484 22.29251049220866 32-33 21.61460886682482 26.55344118680138 29.377917274774646 22.45403267159915 34-35 22.218890994074798 26.55404610845552 29.148158626071268 22.078904271398414 36-37 22.011906814211706 26.403478823921343 29.414773809215294 22.169840552651653 38-39 21.713498074225416 26.76730896710503 29.36735816778182 22.151834790887733 40-41 21.885212840190153 26.801947795885532 29.184227149714836 22.128612214209475 42-43 21.80025630701554 26.584378493046955 28.997134950301078 22.618230249636422 44-45 21.962299473677092 26.61431484116407 28.8526521235469 22.570733561611934 46-47 22.214442170949848 27.055620813657296 28.510348490784832 22.21958852460802 48-49 22.14956645091584 26.84754722906892 28.583734649402093 22.419151670613143 50-51 21.910680332398734 27.110994562585493 28.667042610800518 22.311282494215256 52-53 22.139457629284184 27.44265048786308 27.987764435317043 22.430127447535693 54-55 22.065018855137396 27.053046087080006 28.206181058908125 22.675753998874477 56-57 22.10577389981121 27.189721469149124 28.224011404566557 22.480493226473104 58-59 22.399648485690626 26.974517090317775 28.19415156801295 22.431682855978654 60-61 22.238524103522746 26.97297096341615 28.707249964419173 22.081254968641932 62-63 21.916549801736487 27.294114289019305 28.51971255358448 22.269623355659732 64-65 21.73636290236268 27.411603667478452 28.37165984757019 22.48037358258868 66-67 21.880301888946384 27.260731079555654 28.049287899282227 22.80967913221573 68-69 21.731014539338524 27.131737244236025 28.38901695836843 22.748231258057018 70-71 22.102302985145254 27.233375821247314 28.062340811156115 22.601980382451316 72-73 22.095873531633053 26.96330880593915 28.327581732630748 22.613235929797046 74-75 21.746331606957085 27.69387931390628 27.970675389936943 22.589113689199696 76-77 21.934492074515294 27.19479944878604 27.97977980737559 22.89092866932308 78-79 22.069777294360847 27.281253004663235 28.03553615210807 22.613433548867842 80-81 22.129200080523763 27.35746091743009 27.912977246027143 22.600361756019 82-83 22.004396898586666 27.228699565042024 28.21030304350406 22.556600492867247 84-85 22.049778071512794 27.20126022335544 28.059796772085825 22.68916493304594 86-87 21.945076568355752 27.28424546936139 28.04444644721327 22.726231515069585 88-89 21.973199083403244 27.090038131446466 27.897881027183207 23.038881757967083 90-91 21.878779002080563 26.969963270916423 28.140749754012784 23.010507972990226 92-93 22.146900208303837 26.8698808871558 28.250612012225602 22.732606892314763 94-95 21.92836234526293 26.82748870158863 28.1682397760408 23.075909177107647 96-97 21.505341618358838 27.017710011840222 28.516419904639847 22.960528465161097 98-99 21.69782727654104 27.100397618420768 27.966730301137645 23.235044803900546 100-101 21.698502527330124 26.662891599110356 28.405681076134577 23.232924797424943 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1872.0 1 1071.0 2 292.5 3 308.5 4 266.5 5 260.0 6 409.5 7 827.5 8 1389.5 9 1685.5 10 1544.0 11 1370.5 12 1438.0 13 1504.5 14 1589.0 15 1732.5 16 1812.5 17 1878.5 18 2015.5 19 2164.0 20 2369.0 21 2754.0 22 3267.5 23 3961.5 24 4922.5 25 6132.0 26 7905.5 27 10230.5 28 11924.0 29 13731.5 30 16253.0 31 18376.5 32 20393.0 33 22685.0 34 25460.5 35 28022.5 36 30801.0 37 33719.0 38 35518.0 39 36888.0 40 38476.0 41 40905.5 42 43108.0 43 45392.0 44 47959.0 45 49985.5 46 51486.0 47 54235.5 48 59901.0 49 59606.0 50 53084.5 51 48494.5 52 45974.5 53 44492.0 54 42952.5 55 39821.5 56 35195.5 57 31151.0 58 27794.5 59 24192.5 60 20475.0 61 16762.5 62 13124.5 63 9945.0 64 7531.5 65 5715.0 66 4220.5 67 3280.0 68 2520.5 69 1788.0 70 1300.0 71 922.5 72 682.5 73 511.5 74 376.0 75 269.5 76 166.0 77 104.5 78 66.5 79 47.0 80 35.5 81 21.5 82 18.0 83 12.0 84 9.5 85 13.5 86 10.5 87 8.5 88 8.0 89 4.0 90 2.0 91 2.5 92 4.0 93 3.5 94 2.5 95 5.5 96 5.5 97 6.0 98 7.5 99 6.5 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3703104010746498 2 0.31986123104970254 3 0.3128148388025331 4 0.31858688351563996 5 0.32008611590865477 6 0.2328307906351948 7 0.2164891575513337 8 0.2243601276146613 9 0.21836319804260218 10-11 0.24133893446555366 12-13 0.2274335540203416 14-15 0.22244860631356744 16-17 0.22301081846094797 18-19 0.21757610103626943 20-21 0.20764368643254655 22-23 0.21116688255613125 24-25 0.1995103507004414 26-27 0.20119698714258302 28-29 0.2232357033199002 30-31 0.23594169785070046 32-33 0.22814568940702362 34-35 0.23804062320092112 36-37 0.20700651266551523 38-39 0.20490758731529457 40-41 0.19977271636921895 42-43 0.20989253502206867 44-45 0.21693892726923816 46-47 0.22312326089042409 48-49 0.23106919257340242 50-51 0.23571681299174824 52-53 0.2600043777585876 54-55 0.23080682690462484 56-57 0.19636196267511036 58-59 0.1974114253502207 60-61 0.19043999472270196 62-63 0.20292110439455 64-65 0.19366334436768376 66-67 0.18987778257532145 68-69 0.18669191374016503 70-71 0.19917302341201307 72-73 0.19846088802533104 74-75 0.20322095087315298 76-77 0.2078310904816734 78-79 0.20629437727883324 80-81 0.20479514488581846 82-83 0.19662432834388793 84-85 0.1878538188447515 86-87 0.18935305123776627 88-89 0.19257640088274802 90-91 0.19834844559585493 92-93 0.19096472606025713 94-95 0.1891656471886394 96-97 0.19021510986374976 98-99 0.20348331654193053 100-101 0.18534260458645174 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1334016.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.08139825661535 #Duplication Level Percentage of deduplicated Percentage of total 1 79.72180245679638 48.695191655987955 2 11.713595135593538 14.309655389878822 3 3.6583223854871636 6.703663397370975 4 1.6436356105281678 4.015822452617045 5 0.863379885088025 2.6368225303906225 6 0.5247317189208478 1.9230808260769585 7 0.34799087987215843 1.487903866619891 8 0.24622977340651372 1.2032047081663535 9 0.19501807522349093 1.0720779047968159 >10 1.0037380862621383 10.947858033414176 >50 0.05683575310484433 2.3676536645314576 >100 0.022150349149622522 2.2283597908123753 >500 0.0013461331541915872 0.5890954024004232 >1k 0.001223757412901443 1.8196103769361125 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4897 0.367087051429668 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3684 0.2761586067933218 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3265 0.24474968815966225 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2705 0.2027711811552485 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2466 0.18485535405872192 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1952 0.14632508155824217 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1723 0.129158870658223 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1539 0.11536593264248704 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 7.496161965073882E-5 7 0.0 0.0 0.0 0.0 7.496161965073882E-5 8 0.0 0.0 0.0 0.0 1.4992323930147763E-4 9 0.0 0.0 0.0 0.0 1.4992323930147763E-4 10-11 0.0 0.0 0.0 0.0 1.4992323930147763E-4 12-13 0.0 0.0 0.0 7.496161965073882E-5 1.4992323930147763E-4 14-15 0.0 0.0 0.0 7.496161965073882E-5 1.4992323930147763E-4 16-17 0.0 0.0 0.0 1.8740404912684705E-4 1.8740404912684705E-4 18-19 0.0 0.0 0.0 2.9984647860295527E-4 2.2488485895221647E-4 20-21 0.0 0.0 0.0 2.9984647860295527E-4 2.2488485895221647E-4 22-23 0.0 0.0 0.0 3.748080982536941E-4 2.2488485895221647E-4 24-25 0.0 0.0 0.0 6.746545768566494E-4 2.2488485895221647E-4 26-27 0.0 0.0 0.0 7.870970063327576E-4 2.2488485895221647E-4 28-29 0.0 0.0 0.0 0.0013118283438879293 2.2488485895221647E-4 30-31 0.0 0.0 0.0 0.0029609839762041834 2.2488485895221647E-4 32-33 0.0 0.0 0.0 0.0051723517559009785 2.2488485895221647E-4 34-35 0.0 0.0 0.0 0.007046392247169449 2.2488485895221647E-4 36-37 0.0 0.0 0.0 0.008508143830358856 2.2488485895221647E-4 38-39 0.0 0.0 0.0 0.009445164075993091 2.9984647860295527E-4 40-41 0.0 0.0 0.0 0.010906915659182498 2.9984647860295527E-4 42-43 0.0 0.0 0.0 0.013680495586259835 2.9984647860295527E-4 44-45 0.0 0.0 0.0 0.015592016887353673 2.9984647860295527E-4 46-47 0.0 0.0 0.0 0.018028269526002685 2.9984647860295527E-4 48-49 0.0 0.0 0.0 0.021776350508539627 3.748080982536941E-4 50-51 0.0 0.0 0.0 0.027510914411821148 3.748080982536941E-4 52-53 0.0 0.0 0.0 0.04179110295528689 3.748080982536941E-4 54-55 0.0 0.0 0.0 0.058732428996353864 3.748080982536941E-4 56-57 0.0 0.0 0.0 0.07972168249856074 3.748080982536941E-4 58-59 0.0 0.0 0.0 0.10959388792938016 3.748080982536941E-4 60-61 0.0 0.0 0.0 0.16011801957397812 3.748080982536941E-4 62-63 0.0 0.0 0.0 0.1899152633851468 3.748080982536941E-4 64-65 0.0 0.0 0.0 0.219562583957014 3.748080982536941E-4 66-67 0.0 0.0 0.0 0.2906636801957398 3.748080982536941E-4 68-69 0.0 0.0 0.0 0.3797180843408175 3.748080982536941E-4 70-71 0.0 0.0 0.0 0.4487202552293226 3.748080982536941E-4 72-73 0.0 0.0 0.0 0.5240192021684897 3.748080982536941E-4 74-75 0.0 0.0 0.0 0.6728180171752063 3.748080982536941E-4 76-77 0.0 7.496161965073882E-5 0.0 0.7696684297639609 3.748080982536941E-4 78-79 0.0 7.496161965073882E-5 0.0 0.8575609288044521 3.748080982536941E-4 80-81 0.0 7.496161965073882E-5 0.0 0.9812476012281712 4.122889080790635E-4 82-83 0.0 7.496161965073882E-5 0.0 1.184056263193245 5.247313375551717E-4 84-85 0.0 7.496161965073882E-5 0.0 1.3803432642487046 5.247313375551717E-4 86-87 0.0 7.496161965073882E-5 0.0 1.6547402729802343 5.247313375551717E-4 88-89 0.0 7.496161965073882E-5 0.0 2.018341609096143 5.247313375551717E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 4800 0.0 24.67956 1 TACATGG 4740 0.0 24.373228 2 GTATCAA 8785 0.0 22.853354 1 GAGTACT 4280 0.0 22.027287 12-13 ACATGGG 5000 0.0 21.869707 3 GTGTAGG 560 0.0 21.238863 1 GTACTTT 4630 0.0 20.311632 14-15 AGAGTAC 7020 0.0 20.298365 10-11 AGTACTT 4655 0.0 20.048742 12-13 TCAACGC 10055 0.0 19.954086 4 CAACGCA 10135 0.0 19.84349 5 ATCAACG 10090 0.0 19.789885 3 TATCAAC 10215 0.0 19.640802 2 CATGGGG 2835 0.0 19.621595 4 AACGCAG 10355 0.0 19.450989 6 CGGTACT 125 0.005999002 19.01785 4 GGTATCA 6830 0.0 18.528498 1 GTACCTG 1080 0.0 18.0609 1 CGCAGAG 11165 0.0 17.952738 8 ACGCAGA 11220 0.0 17.908413 7 >>END_MODULE