FastQCFastQC Report
Thu 26 May 2016
SRR937374_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937374_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1334016
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54250.40666678660525807No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46650.3496959556706966No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT45700.3425746018038764No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33390.2502968480138169No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16790.12586055939359048No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT15720.11783966609096143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG47900.022.4216521
GAGTACT48350.022.30122212-13
TACATGG47650.022.1295742
AGTACTT51800.021.41195912-13
ACATGGG47200.021.1329573
AGAGTAC74650.020.87110-11
GGACCGA2056.837454E-720.8515836
GTACTTT52250.020.81611314-15
TCTATAC4150.020.6019043
GTATCAA104350.020.5389731
CTACGTC3302.7284841E-1120.1510223
ATACCGT1200.00476663519.78976
CGACAGA2702.302113E-819.3499286
CCTACGT3455.2750693E-1119.274892
ACGTCTA3305.984475E-1018.7116645
TACCTGG9150.018.6880682
TACTTTT59900.018.55408114-15
TCAACGC118100.017.8170934
CAACGCA117800.017.7818225
ATCAACG118700.017.7670463
Produced by FastQC (version 0.11.5)