##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937374_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1334016 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.219951634763 33.0 31.0 34.0 31.0 34.0 2 32.449645281615815 34.0 31.0 34.0 31.0 34.0 3 32.531991370418346 34.0 31.0 34.0 31.0 34.0 4 36.13456660189983 37.0 35.0 37.0 35.0 37.0 5 35.99007583117444 37.0 35.0 37.0 35.0 37.0 6 36.082214905968144 37.0 35.0 37.0 35.0 37.0 7 36.04567786293418 37.0 35.0 37.0 35.0 37.0 8 36.02254320787757 37.0 35.0 37.0 35.0 37.0 9 37.732285070044135 39.0 37.0 39.0 35.0 39.0 10-11 37.648543195883704 39.0 37.0 39.0 35.0 39.0 12-13 37.56652319012666 39.0 37.0 39.0 35.0 39.0 14-15 38.79953388864901 40.0 38.0 41.0 35.0 41.0 16-17 38.781962135386685 40.0 38.0 41.0 35.0 41.0 18-19 38.77651392487047 40.0 38.0 41.0 35.0 41.0 20-21 38.68002595171272 40.0 38.0 41.0 34.0 41.0 22-23 38.55226286641239 40.0 38.0 41.0 34.0 41.0 24-25 38.40364508371714 40.0 38.0 41.0 34.0 41.0 26-27 38.186309984288044 40.0 38.0 41.0 34.0 41.0 28-29 38.189264221718474 40.0 38.0 41.0 34.0 41.0 30-31 38.34084373800614 40.0 38.0 41.0 34.0 41.0 32-33 38.31341902945692 40.0 38.0 41.0 34.0 41.0 34-35 38.20857396013241 40.0 38.0 41.0 34.0 41.0 36-37 38.064146531975624 40.0 38.0 41.0 33.5 41.0 38-39 37.932277049150834 40.0 37.0 41.0 33.0 41.0 40-41 37.79649531939647 40.0 37.0 41.0 33.0 41.0 42-43 37.599886358184605 40.0 37.0 41.0 33.0 41.0 44-45 37.34237220543082 40.0 37.0 41.0 32.5 41.0 46-47 37.045201481841296 40.0 37.0 41.0 31.5 41.0 48-49 36.752682126751104 40.0 36.0 41.0 31.0 41.0 50-51 36.540699661773175 39.0 36.0 41.0 30.5 41.0 52-53 36.32270639932354 39.0 35.5 41.0 30.0 41.0 54-55 36.12424213802534 39.0 35.0 40.0 30.0 41.0 56-57 35.87649885758492 39.0 35.0 40.0 29.0 41.0 58-59 35.62524025199098 38.0 35.0 40.0 28.5 41.0 60-61 35.28570459424775 38.0 34.5 40.0 28.0 41.0 62-63 34.918785082038 38.0 34.0 40.0 27.5 41.0 64-65 34.582554107297064 37.0 34.0 40.0 27.0 41.0 66-67 34.177145926285746 36.5 34.0 39.0 26.0 41.0 68-69 33.73633149827288 36.0 33.0 39.0 26.0 40.0 70-71 33.34032950129534 35.5 33.0 38.5 26.0 40.0 72-73 32.89524188615429 35.0 32.0 38.0 25.0 40.0 74-75 32.45416209400787 35.0 32.0 37.0 24.0 39.0 76-77 31.159332046991942 34.0 30.5 36.0 22.5 38.5 78-79 31.790913677197274 34.5 31.5 36.0 24.0 39.0 80-81 31.611407959124925 35.0 32.0 36.0 23.0 37.5 82-83 31.269529375959507 34.5 31.0 35.5 22.0 37.0 84-85 30.931933350124737 34.0 31.0 35.0 20.5 37.0 86-87 30.610705943556898 34.0 31.0 35.0 20.0 36.0 88-89 30.25580990033103 34.0 31.0 35.0 18.0 36.0 90-91 29.809009037372867 34.0 30.0 35.0 13.5 35.5 92-93 29.399902624856075 34.0 29.5 35.0 7.0 35.0 94-95 28.993133140831894 34.0 29.0 35.0 2.0 35.0 96-97 28.565519828847627 34.0 29.0 35.0 2.0 35.0 98-99 27.93341196807235 33.5 29.0 35.0 2.0 35.0 100-101 26.324932009810976 32.0 25.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.09116662359348737 1101 2 -0.03988150001146806 1101 3 -0.04860956199009081 1101 4 -0.030057149034718122 1101 5 -0.1831965415475949 1101 6 -0.15569574857688906 1101 7 -0.06594581591739512 1101 8 -0.0853451255208384 1101 9 -0.05556310508192297 1101 10-11 -0.07590465373411348 1101 12-13 -0.05915331510600197 1101 14-15 -0.08154354464659974 1101 16-17 -0.10172864306557727 1101 18-19 -0.05380983631439307 1101 20-21 -0.09389788261539422 1101 22-23 -0.20500992751763647 1101 24-25 -0.0021506627199343598 1101 26-27 -0.17661746156445446 1101 28-29 -0.14855657385281518 1101 30-31 -0.2876745005175749 1101 32-33 -0.22406328039119927 1101 34-35 -0.2800573905887589 1101 36-37 -0.16028824166771471 1101 38-39 -0.1377244281045762 1101 40-41 -0.139517912103166 1101 42-43 -0.18126903867316457 1101 44-45 -0.2390245110990037 1101 46-47 -0.2259247826094466 1101 48-49 -0.2838698534927815 1101 50-51 -0.3750397133829111 1101 52-53 -0.27199918747766105 1101 54-55 -0.21540656360638621 1101 56-57 -0.28334745010043605 1101 58-59 -0.29676480473618483 1101 60-61 -0.2682944110537946 1101 62-63 -0.30125009835432337 1101 64-65 -0.3444563848517035 1101 66-67 -0.31013169678202956 1101 68-69 -0.39344001528585437 1101 70-71 -0.5086201616016339 1101 72-73 -0.6641927564068553 1101 74-75 -0.6266535889666045 1101 76-77 -0.506798984127439 1101 78-79 -0.40622763227517567 1101 80-81 -0.5721821626946699 1101 82-83 -0.5616920147342839 1101 84-85 -0.6195380549034191 1101 86-87 -0.6643000749687715 1101 88-89 -0.6678552852137081 1101 90-91 -0.5476494035833817 1101 92-93 -0.5218373676689758 1101 94-95 -0.5716063287554185 1101 96-97 -0.4796948315162872 1101 98-99 -0.6568639361103088 1101 100-101 -0.37568672023287775 1102 1 0.08451731623585346 1102 2 0.0994813171547051 1102 3 0.06643805633974864 1102 4 -0.0176816589489448 1102 5 -0.05066572058293417 1102 6 0.033737240420101955 1102 7 0.06886565684048662 1102 8 0.05103020737142572 1102 9 0.10270357802187391 1102 10-11 0.07660845066013877 1102 12-13 0.0881865345803945 1102 14-15 0.03137119456020088 1102 16-17 0.07100872587029272 1102 18-19 -0.026441611045321167 1102 20-21 0.09731470384777197 1102 22-23 0.07743976652026419 1102 24-25 0.15631641519798478 1102 26-27 0.10221664705949962 1102 28-29 0.09537626790984888 1102 30-31 0.02236319362583572 1102 32-33 0.09851059652304883 1102 34-35 0.2429107621128992 1102 36-37 0.19082487144756044 1102 38-39 0.21030066687099236 1102 40-41 0.2881519416799492 1102 42-43 0.329827504523017 1102 44-45 0.25712411321549666 1102 46-47 0.3840051639932085 1102 48-49 0.618312713854138 1102 50-51 0.5244475504432629 1102 52-53 0.4819979421257017 1102 54-55 0.49666754021562554 1102 56-57 0.4407908521988446 1102 58-59 0.2930895920794683 1102 60-61 0.37006172161968465 1102 62-63 0.44628890797810783 1102 64-65 0.4946129864765183 1102 66-67 0.5667227963953962 1102 68-69 0.5151313648355185 1102 70-71 0.36067154703265913 1102 72-73 0.42409185860092435 1102 74-75 0.36793453980072144 1102 76-77 0.33169988779376425 1102 78-79 0.47256149854697327 1102 80-81 0.47531140825209306 1102 82-83 0.4217318753422319 1102 84-85 0.2648555361231999 1102 86-87 0.38739734581942287 1102 88-89 0.500873918272756 1102 90-91 0.23668580512600101 1102 92-93 0.10987828110688369 1102 94-95 0.3258095606018827 1102 96-97 0.28375624980821 1102 98-99 0.2937546903521415 1102 100-101 0.3338651544343634 1103 1 0.06384538697069075 1103 2 0.06362268341949573 1103 3 0.02962177043586678 1103 4 -0.010381850263939896 1103 5 -0.06914711476937185 1103 6 -0.012662569126689505 1103 7 -0.011205483034899544 1103 8 -0.0678220750508629 1103 9 -0.02732921267780597 1103 10-11 -0.0831083255961147 1103 12-13 -0.009319207023722242 1103 14-15 -0.03418408211401669 1103 16-17 0.00811015297669826 1103 18-19 -0.04311246093200083 1103 20-21 -0.05279996665094444 1103 22-23 0.02708115863843119 1103 24-25 0.014308663493814322 1103 26-27 -0.13297610063739995 1103 28-29 -0.2014773222624271 1103 30-31 -0.09111751113722022 1103 32-33 0.005824624565349268 1103 34-35 0.05020992446348771 1103 36-37 0.07311309997417936 1103 38-39 0.03595195652361127 1103 40-41 -0.00954915450322602 1103 42-43 0.07564383567030575 1103 44-45 0.13831653559073231 1103 46-47 -0.02563809172254139 1103 48-49 -0.062078146115354116 1103 50-51 -0.0568112289326379 1103 52-53 0.06554339882560356 1103 54-55 -0.13181701023724202 1103 56-57 -0.1046176419281295 1103 58-59 -0.13650923891701439 1103 60-61 -0.15133140913807352 1103 62-63 -0.08092931175836071 1103 64-65 -0.17881819829756296 1103 66-67 -0.1955626744376886 1103 68-69 -0.10585193603746035 1103 70-71 -0.12961566244002398 1103 72-73 -0.07863028653992643 1103 74-75 -0.24739575863342367 1103 76-77 -0.3675607056274153 1103 78-79 -0.19765821206755874 1103 80-81 -0.16698988700209227 1103 82-83 -0.32738614747768224 1103 84-85 -0.21213899804203962 1103 86-87 -0.03988033668387203 1103 88-89 -0.05292426595840638 1103 90-91 -0.006222290428080868 1103 92-93 -0.012889710688980927 1103 94-95 -0.1822184515319698 1103 96-97 -0.25473835114491905 1103 98-99 -0.431799056663948 1103 100-101 -0.09294366630694029 1104 1 -0.05528410971103881 1104 2 -0.0305465421154949 1104 3 -0.04641807039031676 1104 4 5.02940368235727E-4 1104 5 -0.044898248495186976 1104 6 -0.04500255551766941 1104 7 -0.07501766568856993 1104 8 -0.1459791730573201 1104 9 -0.12075607928791499 1104 10-11 -0.12177935777109639 1104 12-13 -0.06664732320854938 1104 14-15 -0.06753492018825824 1104 16-17 -0.09174304406548828 1104 18-19 -0.11124488721289794 1104 20-21 -0.10031780156840853 1104 22-23 -0.1115184369670672 1104 24-25 -0.17772408643189408 1104 26-27 -0.062331820300265406 1104 28-29 -0.030360763836249305 1104 30-31 -0.16306899456307633 1104 32-33 -0.06479610377542855 1104 34-35 0.006110089838877286 1104 36-37 0.04740845116000969 1104 38-39 -0.08911856488259673 1104 40-41 -0.06935049947026073 1104 42-43 -0.08543795731760184 1104 44-45 0.06846938939285963 1104 46-47 0.07375765825915437 1104 48-49 0.05745240741846658 1104 50-51 0.1278921397295889 1104 52-53 0.16686691335409165 1104 54-55 0.23400915072813433 1104 56-57 0.155097758439652 1104 58-59 0.0026546625209817876 1104 60-61 -0.031229955212779714 1104 62-63 0.06521096016356154 1104 64-65 0.11654388874845978 1104 66-67 0.009045630292312978 1104 68-69 -0.05869828227495333 1104 70-71 -0.0010387198175365597 1104 72-73 -0.015844163430010383 1104 74-75 -0.10326803844513677 1104 76-77 -0.09373200696807515 1104 78-79 -0.051497483554516776 1104 80-81 0.003190647797797652 1104 82-83 -0.017090210587340948 1104 84-85 -0.1163893662606803 1104 86-87 0.043766126048524256 1104 88-89 0.17092332813114197 1104 90-91 0.09833915255832437 1104 92-93 0.036426285503939226 1104 94-95 0.1073351748833069 1104 96-97 0.21169830448236482 1104 98-99 0.3715680174836571 1104 100-101 0.6389198160939351 1105 1 -0.04563201384461735 1105 2 -0.049317549589346754 1105 3 -0.04355269660652539 1105 4 -0.10849756388136456 1105 5 -0.20067654160325787 1105 6 -0.08464275446450387 1105 7 -0.1316399799273711 1105 8 -0.13126463592053028 1105 9 -0.06408769433337369 1105 10-11 -0.0888951202765611 1105 12-13 -0.06763615808295498 1105 14-15 -0.12417998886592585 1105 16-17 -0.15421999115019247 1105 18-19 -0.09223046342692953 1105 20-21 -0.13628259679952492 1105 22-23 -0.1433257714811944 1105 24-25 -0.12454906968209656 1105 26-27 -0.22154828102990365 1105 28-29 -0.27080705984902664 1105 30-31 -0.3009816821291764 1105 32-33 -0.15658905523684297 1105 34-35 -0.044695234699815956 1105 36-37 -0.18296760105721432 1105 38-39 -0.13827784455010317 1105 40-41 -0.1105151053619764 1105 42-43 -0.4920870290910031 1105 44-45 -0.4076790425875245 1105 46-47 -0.25912545555939204 1105 48-49 -0.29929997231659655 1105 50-51 -0.25379531260197297 1105 52-53 -0.26226298218617927 1105 54-55 -0.13885196713283676 1105 56-57 -0.23255795847265404 1105 58-59 -0.16582594493826264 1105 60-61 -0.352870971633898 1105 62-63 -0.3333980694158001 1105 64-65 -0.12968905771258932 1105 66-67 -0.2319314621679638 1105 68-69 -0.33808542938802333 1105 70-71 -0.33626315302833376 1105 72-73 -0.3139430441082638 1105 74-75 -0.2784384509325477 1105 76-77 -0.37830562108931076 1105 78-79 -0.22735291701955518 1105 80-81 -0.24895620541780872 1105 82-83 -0.3659911852358064 1105 84-85 -0.31068895794491524 1105 86-87 -0.29050908186664515 1105 88-89 -0.296950072913031 1105 90-91 -0.2181954587691699 1105 92-93 -0.24450128093691248 1105 94-95 -0.22033717243370532 1105 96-97 -0.4326375959824169 1105 98-99 -0.5018775523535055 1105 100-101 -0.37162995643190655 1106 1 -0.1496776464377163 1106 2 -0.17761223189496889 1106 3 -0.13143020780088221 1106 4 -0.14119152511182875 1106 5 -0.23506574990846474 1106 6 -0.21377660286643874 1106 7 -0.1524690910659814 1106 8 -0.08550831359178801 1106 9 -0.21260192554414914 1106 10-11 -0.35795153610701647 1106 12-13 -0.49556936309289057 1106 14-15 -0.2796315144160175 1106 16-17 -0.1915320284381039 1106 18-19 -0.23603781544911584 1106 20-21 -0.43139811134867045 1106 22-23 -0.45926789126048817 1106 24-25 -0.20471010562466319 1106 26-27 -0.10121377357403816 1106 28-29 -0.2747546487735377 1106 30-31 -0.29702046732739973 1106 32-33 -0.27397348195545845 1106 34-35 -0.2554410400439835 1106 36-37 -0.2678771769165422 1106 38-39 -0.3876978050507205 1106 40-41 -0.37117672784373923 1106 42-43 -0.6630830180712692 1106 44-45 -0.4267237902816916 1106 46-47 -0.36096996080691923 1106 48-49 -0.4933590898296103 1106 50-51 -0.3529509780694511 1106 52-53 -0.18820571278066467 1106 54-55 -0.34286439643881295 1106 56-57 -0.33737635058781024 1106 58-59 -0.4077284932905485 1106 60-61 -0.2420769275518353 1106 62-63 -0.4030134594526231 1106 64-65 -0.5719902021296619 1106 66-67 -0.4850210123730605 1106 68-69 -0.45449020251717087 1106 70-71 -0.2683032287694189 1106 72-73 -0.4246974635127643 1106 74-75 -0.4895109640924815 1106 76-77 -0.4506651375116242 1106 78-79 -0.4153933122639657 1106 80-81 -0.3181524822643098 1106 82-83 -0.6685541031156212 1106 84-85 -0.640451476132327 1106 86-87 -0.33086109935788954 1106 88-89 -0.6641400567969065 1106 90-91 -0.5178269336059351 1106 92-93 -0.6303815830868267 1106 94-95 -0.8899300092943676 1106 96-97 -0.6697580878922444 1106 98-99 -0.4943241177710327 1106 100-101 -0.42922575652932693 1107 1 -0.04537228207839661 1107 2 -0.01439078989884024 1107 3 -0.0070687920345875455 1107 4 -0.011094725829103425 1107 5 -0.1007377373970968 1107 6 -0.032113717605327 1107 7 -0.09175713950825326 1107 8 -0.03770047142997157 1107 9 -0.10690450713808985 1107 10-11 -0.07038160192328036 1107 12-13 -0.057339537554490505 1107 14-15 0.02949713091432926 1107 16-17 0.023903524696102352 1107 18-19 0.00614652446315489 1107 20-21 -0.026592017058916895 1107 22-23 -0.046639842932343356 1107 24-25 -0.03320632807939461 1107 26-27 0.1006055306271989 1107 28-29 -0.012300766647989292 1107 30-31 0.004106133056410499 1107 32-33 -0.034303197693795084 1107 34-35 -0.06146148078165936 1107 36-37 0.03566176887525785 1107 38-39 0.1522357174032436 1107 40-41 -0.09640986416801667 1107 42-43 -0.0492846977336896 1107 44-45 -0.05696392380072268 1107 46-47 0.051392902400188234 1107 48-49 -0.027323597318734016 1107 50-51 -0.08352541267966984 1107 52-53 0.033537553783084206 1107 54-55 0.15510257357949797 1107 56-57 0.24860354703730536 1107 58-59 0.2349993418488978 1107 60-61 0.1376654803492059 1107 62-63 0.1608844060085559 1107 64-65 0.17614385129876098 1107 66-67 0.16702556219045306 1107 68-69 0.12922169096837877 1107 70-71 -0.13683602663021333 1107 72-73 0.013555355925369383 1107 74-75 0.08628162891784541 1107 76-77 0.02928032162956029 1107 78-79 -0.03544664468425651 1107 80-81 0.16563564355159244 1107 82-83 0.26957820814725864 1107 84-85 0.007996469203284562 1107 86-87 0.1425065939108734 1107 88-89 0.2644551569137654 1107 90-91 0.34622440354807793 1107 92-93 0.3998661367407301 1107 94-95 0.43683713903575594 1107 96-97 0.7191749985168165 1107 98-99 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2316 58-59 -0.41447459358691674 2316 60-61 -0.46349237470813165 2316 62-63 -0.6917137975428531 2316 64-65 -0.4620704957544106 2316 66-67 -0.5568786465491087 2316 68-69 -0.34832437453263054 2316 70-71 -0.2883575655001138 2316 72-73 -0.12587573151264309 2316 74-75 -0.24529573948795047 2316 76-77 -0.420561758628466 2316 78-79 -0.36843982603099334 2316 80-81 -0.5659336950368079 2316 82-83 -0.37283171849143315 2316 84-85 -0.3943051887309572 2316 86-87 -0.3082879119096269 2316 88-89 -0.4802931194636244 2316 90-91 -0.35671807842386727 2316 92-93 -0.5114896979253309 2316 94-95 -0.5587760155895332 2316 96-97 -0.31107789612082826 2316 98-99 -0.3631655249622341 2316 100-101 -0.8135364181496918 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 5.0 5 29.0 6 41.0 7 113.0 8 281.0 9 519.0 10 881.0 11 1473.0 12 2294.0 13 3189.0 14 4259.0 15 5688.0 16 7883.0 17 11257.0 18 9953.0 19 6228.0 20 5591.0 21 5603.0 22 5760.0 23 6428.0 24 7515.0 25 9037.0 26 10951.0 27 13672.0 28 17616.0 29 21780.0 30 27575.0 31 35572.0 32 45787.0 33 60730.0 34 84802.0 35 123623.0 36 195318.0 37 307995.0 38 267058.0 39 27501.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.71189262646959 19.87934277511617 15.42909513081199 21.979669467602246 2 15.74164028017655 20.81774131644598 39.67591093360199 23.764707469775477 3 19.608910237958167 24.417548215313758 30.136220255229322 25.837321291498753 4 13.116259475148723 17.206615213010938 37.29085708117444 32.3862682306659 5 13.568613397528795 37.82311711348337 34.39062918199518 14.217640306992651 6 31.845570068125117 36.986437943772785 17.050170312799846 14.117821675302245 7 28.556554044329303 32.82157035597774 20.508599597006334 18.113276002686625 8 26.393161701208985 35.530233520437534 19.173533151026675 18.90307162732681 9 25.926675542122435 17.11051441661869 20.407551333717137 36.55525870754174 10-11 25.023837795048937 26.98584574697755 27.239778233544428 20.750538224429093 12-13 26.08951842549046 24.0327709210365 27.998394214373878 21.879316439099156 14-15 22.57116856169641 25.785222965841488 26.44863329975053 25.19497517271157 16-17 21.8848949337939 29.065131152849744 27.050050374208407 21.999923539147957 18-19 21.857533942621377 27.75356517463059 29.293839054404145 21.09506182834389 20-21 22.642307138744965 26.563511981865283 29.485328511801956 21.308852367587793 22-23 22.340779610194904 26.306184407796103 29.478073463268366 21.874962518740627 24-25 22.421841823465275 26.57844640536668 29.519429959082 21.480281812086048 26-27 22.131708534765227 26.727241787988078 29.71627994491717 21.42476973232953 28-29 21.80840178143734 27.30249126860318 29.474414514771226 21.41469243518826 30-31 22.181167918722974 26.647004079068637 29.407037677131015 21.76479032507737 32-33 22.03905350460564 26.724604502494724 29.425696543369796 21.810645449529844 34-35 22.55055411629246 26.67096946363462 29.306882376223374 21.47159404384955 36-37 22.450943131738597 26.55926141649294 29.430785157266083 21.55901029450238 38-39 22.10340765428184 26.956664166688217 29.434678786502218 21.505249392527727 40-41 22.166167546841677 27.013564315243833 29.152670697106103 21.667597440808386 42-43 22.151295406708787 26.83425204155647 28.980674327462474 22.033778224272265 44-45 22.394717302759975 26.809845912584972 28.843705339255806 21.951731445399247 46-47 22.692493943101134 27.352145701400882 28.42926921416235 21.526091141335634 48-49 22.61794104109852 27.12306617471781 28.484858479746087 21.774134304437585 50-51 22.366935628957975 27.43572790731146 28.515999808098254 21.681336655632315 52-53 22.724037443427253 27.761434019546293 27.685685104150075 21.82884343287638 54-55 22.473456090481672 27.48224159230474 27.917506236806755 22.126796080406834 56-57 22.630163356361543 27.61571075609288 27.906824206006526 21.84730168153905 58-59 22.947850700441375 27.257769022260604 27.94902759786989 21.84535267942813 60-61 22.594031855689884 27.375083957014006 28.52979274611399 21.501091441182115 62-63 22.262026842256766 27.65780170552677 28.358955214929953 21.72121623728651 64-65 22.239913164459796 27.7373359839762 28.042991988102088 21.979758863461907 66-67 22.39362196555364 27.54239829207446 27.839133863461907 22.22484587891 68-69 22.278930687488003 27.501394286125503 27.988382448186528 22.231292578199962 70-71 22.6714672087891 27.565336547687586 27.7976051261754 21.96559111734792 72-73 22.61682018806371 27.312228638936865 27.997152957685667 22.073798215313758 74-75 22.232192117635773 28.06019567981194 27.60735253550182 22.10025966705047 76-77 22.410863138073307 27.68553750479754 27.59363455910574 22.30996479802341 78-79 22.56363491892151 27.670057930339663 27.671107393014776 22.095199757724046 80-81 22.603289615716754 27.74813045720591 27.67942813279601 21.969151794281327 82-83 22.486329755275538 27.748319822070368 27.766235723032167 21.99911469962193 84-85 22.456323546829342 27.722760031004174 27.761215399538536 22.059701022627948 86-87 22.234890735943196 27.790108964210326 27.651355006236805 22.323645293609673 88-89 22.347782935137207 27.635013373152944 27.509565102667434 22.50763858904241 90-91 22.519497440949163 27.442268607751895 27.671259988619777 22.366973962679165 92-93 22.64493018440702 27.357412432059437 27.82151286546222 22.176144518071318 94-95 22.401305531567836 27.472833909038574 27.748580226923814 22.377280332469777 96-97 21.990890663980043 27.705702180483595 28.024438987238533 22.27896816829783 98-99 22.177744144354854 27.78059513917078 27.417923890762342 22.623736825712026 100-101 21.849236578983177 27.783644335885004 27.65491743147466 22.712201653657154 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 321.0 1 236.5 2 184.0 3 223.0 4 206.5 5 204.0 6 387.5 7 881.5 8 1456.0 9 1757.0 10 1645.0 11 1464.0 12 1544.0 13 1655.5 14 1771.5 15 1890.5 16 1912.0 17 2049.5 18 2213.0 19 2274.5 20 2483.5 21 2908.0 22 3399.5 23 4129.0 24 5066.0 25 6052.5 26 7696.0 27 9953.0 28 11723.0 29 13857.5 30 16490.0 31 18364.5 32 20232.0 33 22694.5 34 25654.0 35 27879.5 36 30903.0 37 34198.0 38 35956.0 39 37092.0 40 38308.0 41 40863.0 42 43597.5 43 46265.0 44 48989.0 45 50461.5 46 51181.0 47 54688.5 48 61198.5 49 60216.0 50 53351.5 51 48995.0 52 46164.5 53 44395.5 54 42897.5 55 39770.0 56 34720.5 57 30429.5 58 27298.0 59 24010.5 60 19959.5 61 16107.5 62 12793.0 63 9602.5 64 7068.5 65 5244.0 66 3831.5 67 3046.0 68 2329.0 69 1562.0 70 1062.0 71 722.5 72 580.0 73 462.0 74 358.5 75 274.5 76 171.0 77 89.0 78 41.5 79 22.5 80 12.5 81 7.0 82 5.0 83 4.5 84 3.0 85 2.0 86 1.5 87 2.0 88 2.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04887497601228171 2 0.0 3 0.0 4 0.0 5 0.012968360199577816 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0067090649587411245 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001199385914411821 24-25 4.8725052772980235E-4 26-27 0.0018740404912684703 28-29 0.0027735799270773363 30-31 7.870970063327575E-4 32-33 0.0 34-35 0.0 36-37 2.248848589522165E-4 38-39 3.373272884283247E-4 40-41 7.496161965073882E-5 42-43 0.012631032911149493 44-45 3.748080982536941E-5 46-47 0.0 48-49 1.4992323930147763E-4 50-51 0.0 52-53 2.623656687775859E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.122889080790635E-4 84-85 1.4992323930147763E-4 86-87 0.0 88-89 0.0 90-91 0.009220279217040874 92-93 0.0028110607369027057 94-95 0.0 96-97 0.0 98-99 0.00191152130109384 100-101 0.013830418825561313 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1334016.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.8399411783721 #Duplication Level Percentage of deduplicated Percentage of total 1 78.18705172941395 46.00521524669055 2 12.507924863544575 14.719311264689214 3 3.9469430476713487 6.9671369027810055 4 1.7450511886956683 4.107148371844058 5 0.9341238969158857 2.748189757392123 6 0.5956013742832764 2.1027089897151345 7 0.3892406268096587 1.6032026909999104 8 0.2953120595847927 1.3900915372186495 9 0.2148047168947678 1.137518721623251 >10 1.0926101668926458 11.539121857199893 >50 0.062139284983801744 2.4688736094225856 >100 0.026531258775748724 2.6298773589026734 >500 0.0012694216828228325 0.5648261758320084 >1k 0.0012694216828228325 1.6115696336619165 >5k 1.2694216828228324E-4 0.40520788202706237 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5425 0.40666678660525807 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4665 0.3496959556706966 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4570 0.3425746018038764 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3339 0.2502968480138169 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1679 0.12586055939359048 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1572 0.11783966609096143 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.496161965073882E-5 2 0.0 0.0 0.0 0.0 7.496161965073882E-5 3 0.0 0.0 0.0 0.0 7.496161965073882E-5 4 0.0 0.0 0.0 0.0 7.496161965073882E-5 5 0.0 0.0 0.0 0.0 7.496161965073882E-5 6 0.0 0.0 0.0 0.0 1.4992323930147763E-4 7 0.0 0.0 0.0 0.0 1.4992323930147763E-4 8 0.0 0.0 0.0 0.0 1.4992323930147763E-4 9 0.0 0.0 0.0 0.0 1.4992323930147763E-4 10-11 0.0 0.0 0.0 7.496161965073882E-5 1.4992323930147763E-4 12-13 0.0 0.0 0.0 7.496161965073882E-5 1.4992323930147763E-4 14-15 0.0 0.0 0.0 7.496161965073882E-5 1.4992323930147763E-4 16-17 0.0 0.0 0.0 1.8740404912684705E-4 1.4992323930147763E-4 18-19 0.0 0.0 0.0 2.9984647860295527E-4 1.4992323930147763E-4 20-21 0.0 0.0 0.0 2.9984647860295527E-4 1.4992323930147763E-4 22-23 0.0 0.0 0.0 3.748080982536941E-4 1.8740404912684705E-4 24-25 0.0 0.0 0.0 7.121353866820188E-4 2.9984647860295527E-4 26-27 0.0 0.0 0.0 7.870970063327576E-4 2.9984647860295527E-4 28-29 0.0 0.0 0.0 0.0013118283438879293 2.9984647860295527E-4 30-31 0.0 0.0 0.0 0.002998464786029553 3.373272884283247E-4 32-33 0.0 0.0 0.0 0.0051723517559009785 3.748080982536941E-4 34-35 0.0 0.0 0.0 0.00700891143734408 3.748080982536941E-4 36-37 0.0 0.0 0.0 0.008508143830358856 3.748080982536941E-4 38-39 0.0 0.0 0.0 0.00952012569564383 3.748080982536941E-4 40-41 0.0 0.0 0.0 0.010869434849357129 4.122889080790635E-4 42-43 0.0 0.0 0.0 0.013605533966609096 4.4976971790443293E-4 44-45 0.0 0.0 0.0 0.015442093648052196 4.872505277298023E-4 46-47 0.0 0.0 0.0 0.018028269526002685 5.247313375551717E-4 48-49 0.0 0.0 0.0 0.021701388888888888 5.247313375551717E-4 50-51 0.0 0.0 0.0 0.027473433601995778 5.247313375551717E-4 52-53 0.0 0.0 0.0 0.04224087267319132 5.996929572059105E-4 54-55 0.0 0.0 0.0 0.059519526002686625 6.746545768566494E-4 56-57 0.0 0.0 0.0 0.08058374112454422 6.746545768566494E-4 58-59 0.0 0.0 0.0 0.11094319708309346 6.746545768566494E-4 60-61 0.0 0.0 0.0 0.16139236710804067 6.746545768566494E-4 62-63 0.0 0.0 0.0 0.1912645725388601 6.746545768566494E-4 64-65 0.0 0.0 0.0 0.2209493739205527 6.746545768566494E-4 66-67 0.0 0.0 0.0 0.2924252782575322 6.746545768566494E-4 68-69 0.0 0.0 0.0 0.38159212483208593 6.746545768566494E-4 70-71 0.0 0.0 0.0 0.4509316230090194 6.746545768566494E-4 72-73 0.0 0.0 0.0 0.5259682042794089 6.746545768566494E-4 74-75 0.0 0.0 0.0 0.6758164819612359 6.746545768566494E-4 76-77 0.0 0.0 0.0 0.7728542985991173 6.746545768566494E-4 78-79 0.0 0.0 0.0 0.8626957997505278 6.746545768566494E-4 80-81 0.0 0.0 0.0 0.9880316278065631 6.746545768566494E-4 82-83 0.0 0.0 0.0 1.1945883707541738 7.496161965073882E-4 84-85 0.0 0.0 0.0 1.3942861255037422 7.496161965073882E-4 86-87 0.0 0.0 0.0 1.6731433506044906 7.496161965073882E-4 88-89 0.0 0.0 0.0 2.0427416162924583 7.496161965073882E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 4790 0.0 22.421652 1 GAGTACT 4835 0.0 22.301222 12-13 TACATGG 4765 0.0 22.129574 2 AGTACTT 5180 0.0 21.411959 12-13 ACATGGG 4720 0.0 21.132957 3 AGAGTAC 7465 0.0 20.871 10-11 GGACCGA 205 6.837454E-7 20.851583 6 GTACTTT 5225 0.0 20.816113 14-15 TCTATAC 415 0.0 20.601904 3 GTATCAA 10435 0.0 20.538973 1 CTACGTC 330 2.7284841E-11 20.151022 3 ATACCGT 120 0.004766635 19.7897 6 CGACAGA 270 2.302113E-8 19.349928 6 CCTACGT 345 5.2750693E-11 19.27489 2 ACGTCTA 330 5.984475E-10 18.711664 5 TACCTGG 915 0.0 18.688068 2 TACTTTT 5990 0.0 18.554081 14-15 TCAACGC 11810 0.0 17.817093 4 CAACGCA 11780 0.0 17.781822 5 ATCAACG 11870 0.0 17.767046 3 >>END_MODULE