FastQCFastQC Report
Thu 26 May 2016
SRR937373_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937373_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1521467
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54170.35603795547323736No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41200.27079128236103706No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT38060.2501533059869192No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29560.1942861724901033No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT26280.17272803156427316No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24250.15938564556444537No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT19560.12856013308208458No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC15840.10411004642230162No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCGG600.00393267531.697325
GTACATG54800.026.2092651
TACATGG54500.025.8214342
ACATGGG53200.024.3961813
GTATCAA97150.023.3998761
GAGTACT48650.023.13965812-13
GCGTATC1050.002180878322.646921
GTACTTT51800.021.54805414-15
ACCGGCG1553.8784325E-521.4723785
GTCTACG1353.7652755E-421.1371251
AGTACTT51450.020.95713412-13
AGAGTAC79600.020.85573810-11
TCGATTG704.8609014E-420.35301884-85
CAACGCA111450.020.3494225
TCAACGC111500.020.34034
ATCAACG111900.020.2230993
AACGCAG113650.019.978896
GGTATCA75200.019.921461
TATCAAC114250.019.891012
TCTACGG1456.110069E-419.67292
Produced by FastQC (version 0.11.5)