##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937373_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1521467 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.404161247007 31.0 31.0 34.0 30.0 34.0 2 31.499793948866454 33.0 31.0 34.0 30.0 34.0 3 31.547959962325834 33.0 31.0 34.0 28.0 34.0 4 35.17292258064092 37.0 35.0 37.0 33.0 37.0 5 35.16223158307081 37.0 35.0 37.0 33.0 37.0 6 35.20705214112432 37.0 35.0 37.0 32.0 37.0 7 35.14056433691957 37.0 35.0 37.0 32.0 37.0 8 35.1238843826386 37.0 35.0 37.0 32.0 37.0 9 36.70503139404272 39.0 37.0 39.0 32.0 39.0 10-11 36.58967036419456 39.0 37.0 39.0 32.0 39.0 12-13 36.45955613891067 39.0 37.0 39.0 32.0 39.0 14-15 37.560158386609764 40.0 37.0 41.0 32.0 41.0 16-17 37.49748795077383 40.0 37.0 41.0 32.0 41.0 18-19 37.441198198843615 40.0 37.0 41.0 32.0 41.0 20-21 37.36526260510415 40.0 37.0 41.0 32.0 41.0 22-23 37.21361127122705 40.0 37.0 41.0 31.0 41.0 24-25 37.04195063054276 39.0 36.0 41.0 31.0 41.0 26-27 36.864340797401454 39.0 36.0 41.0 30.5 41.0 28-29 36.667390091273745 39.0 36.0 40.5 30.0 41.0 30-31 36.68570202311322 39.0 36.0 41.0 30.0 41.0 32-33 36.53965284820505 39.0 36.0 41.0 30.0 41.0 34-35 36.32038683717754 39.0 35.0 40.0 30.0 41.0 36-37 36.32491273225118 39.0 35.0 41.0 30.0 41.0 38-39 36.303781153321104 39.0 35.0 41.0 30.0 41.0 40-41 36.150567182857074 39.0 35.0 41.0 29.5 41.0 42-43 35.91603695643744 39.0 35.0 41.0 28.5 41.0 44-45 35.69934477711314 39.0 35.0 40.0 27.5 41.0 46-47 35.393139318828474 39.0 35.0 40.0 26.5 41.0 48-49 35.094711222786955 38.5 34.5 40.0 25.5 41.0 50-51 34.186585381082864 37.5 33.5 39.5 23.5 40.5 52-53 34.34470941532087 38.0 33.5 39.5 24.0 40.5 54-55 34.692602599990664 38.0 34.0 40.0 24.0 41.0 56-57 34.446929838110194 38.0 34.0 40.0 23.5 41.0 58-59 34.18783910528457 38.0 34.0 40.0 23.0 41.0 60-61 33.88025405743272 38.0 33.0 40.0 22.0 41.0 62-63 33.55133269403806 37.0 33.0 40.0 21.5 41.0 64-65 33.18970966836612 37.0 32.5 39.5 20.0 41.0 66-67 32.772068667936935 36.0 32.0 39.0 18.5 41.0 68-69 32.25156805898517 35.5 31.0 39.0 16.5 40.0 70-71 31.802048943552506 35.0 31.0 38.5 13.0 40.0 72-73 31.379880733528893 35.0 31.0 37.5 9.5 40.0 74-75 30.904901650840934 35.0 30.0 37.0 7.5 39.0 76-77 30.45767407377222 34.5 30.0 36.5 6.0 39.0 78-79 29.9623504814761 34.0 29.5 36.0 2.0 38.5 80-81 29.475031663519484 34.0 29.0 35.5 2.0 37.5 82-83 29.00939159377101 34.0 29.0 35.0 2.0 37.0 84-85 28.51561387792177 34.0 28.0 35.0 2.0 36.5 86-87 28.068970605343395 34.0 27.0 35.0 2.0 36.0 88-89 27.651365754235876 33.5 26.0 35.0 2.0 36.0 90-91 27.173261727004267 33.0 26.0 35.0 2.0 35.0 92-93 26.6895233350444 33.0 25.0 35.0 2.0 35.0 94-95 26.102318683218236 32.5 24.5 35.0 2.0 35.0 96-97 25.439995083692253 32.0 21.5 34.5 2.0 35.0 98-99 24.681735128004746 32.0 10.0 34.0 2.0 35.0 100-101 22.8291290576792 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12249655005737736 1101 2 0.1629663470217757 1101 3 0.23903868898883118 1101 4 0.13246448643023712 1101 5 0.249122267937409 1101 6 0.042951861995533136 1101 7 0.17393246938524953 1101 8 0.17396334615609277 1101 9 0.04638420395045273 1101 10-11 0.12005977869682027 1101 12-13 0.17159067207395395 1101 14-15 0.23177537291584116 1101 16-17 0.18076343525466854 1101 18-19 0.2182393627915289 1101 20-21 0.36997403679381335 1101 22-23 0.20409489036482142 1101 24-25 0.2620415976756547 1101 26-27 0.3936914722805085 1101 28-29 0.25639553504537815 1101 30-31 0.2694424555335786 1101 32-33 0.2258895612214431 1101 34-35 0.1070568563043679 1101 36-37 0.23257548945611717 1101 38-39 0.29080230183116385 1101 40-41 0.061552178527769286 1101 42-43 0.1847158537591227 1101 44-45 0.18336442213519177 1101 46-47 0.1945721709590842 1101 48-49 0.2493334319907774 1101 50-51 0.2066852782156019 1101 52-53 0.1754418574833636 1101 54-55 0.17204507193832796 1101 56-57 0.14100634272199386 1101 58-59 0.1519229989895905 1101 60-61 0.0846453279183379 1101 62-63 0.23856155614144825 1101 64-65 0.1516309556383888 1101 66-67 0.2888480211218294 1101 68-69 0.11976566332649696 1101 70-71 0.11082417864686533 1101 72-73 0.1694557167834354 1101 74-75 0.2751366901547918 1101 76-77 0.32492014818559767 1101 78-79 0.29901960172647435 1101 80-81 0.31668294103447536 1101 82-83 0.3201716667050576 1101 84-85 0.2706986588442071 1101 86-87 0.5524068061600254 1101 88-89 0.484428039108618 1101 90-91 0.5684943910732265 1101 92-93 0.6293470693412928 1101 94-95 0.6950830280825784 1101 96-97 0.5325906658612247 1101 98-99 0.6702825779962502 1101 100-101 0.8598363041982644 1102 1 -0.0025138697931303966 1102 2 -0.21718324601231132 1102 3 -0.10770743611318068 1102 4 -0.20439976118451852 1102 5 -0.0507810975971239 1102 6 -0.05048460984823322 1102 7 -0.01486878984724882 1102 8 0.11651189236825843 1102 9 0.18900134107980193 1102 10-11 -0.054235890864759995 1102 12-13 0.03233104233111561 1102 14-15 0.05617246235571827 1102 16-17 0.10156794033492389 1102 18-19 0.1606116720259365 1102 20-21 0.13786414827848859 1102 22-23 0.23660212251292023 1102 24-25 0.19876107368838802 1102 26-27 0.2345912894056994 1102 28-29 0.26353454768607065 1102 30-31 0.31004282416473217 1102 32-33 0.25820820693932234 1102 34-35 0.24679065356545493 1102 36-37 0.3737651070656156 1102 38-39 0.4971462155578905 1102 40-41 0.447353318382234 1102 42-43 0.22752458749772586 1102 44-45 0.2904399637358708 1102 46-47 0.5206132812571909 1102 48-49 0.4568363932865651 1102 50-51 0.4780533042482489 1102 52-53 0.49745228659596563 1102 54-55 0.5537158345067184 1102 56-57 0.5773299930554785 1102 58-59 0.49886542892960506 1102 60-61 0.5504355436529806 1102 62-63 0.5895811063538119 1102 64-65 0.53635460576605 1102 66-67 0.6062683131246374 1102 68-69 0.6583820152364908 1102 70-71 0.3882604168801542 1102 72-73 0.1930180861493831 1102 74-75 0.2857156685051727 1102 76-77 0.44599598102752935 1102 78-79 0.48830339008567236 1102 80-81 0.4184642980952269 1102 82-83 0.4865952035264911 1102 84-85 0.527918714766642 1102 86-87 0.5723879629538224 1102 88-89 0.7331079340971058 1102 90-91 0.5793046643769024 1102 92-93 0.2950364569422348 1102 94-95 0.16986313064478153 1102 96-97 0.6175747093395323 1102 98-99 0.9011141695422253 1102 100-101 1.1457793855718954 1103 1 -0.023051395148101506 1103 2 -0.06303111619486756 1103 3 0.02267998782190972 1103 4 -0.025831809866062372 1103 5 -0.20381456615696436 1103 6 -0.15377061390502433 1103 7 0.016349262891083072 1103 8 0.024696476546758106 1103 9 -0.02662137697293332 1103 10-11 -0.0017505967471151962 1103 12-13 -0.042149180792613095 1103 14-15 -0.023414251640225814 1103 16-17 -0.0017525464804961643 1103 18-19 -0.04131682695986427 1103 20-21 0.07377388458680656 1103 22-23 -0.003893313592556069 1103 24-25 -0.0834017052162821 1103 26-27 -0.1438691568417667 1103 28-29 -0.2499715375066316 1103 30-31 -0.22438060382715008 1103 32-33 -0.33374108312153083 1103 34-35 -0.34489494481182703 1103 36-37 -0.11399858461795276 1103 38-39 -0.11824982906685477 1103 40-41 0.020899971526247896 1103 42-43 -0.05621121371931537 1103 44-45 -0.17741754133512444 1103 46-47 -0.3176083211768841 1103 48-49 -0.2653268927174892 1103 50-51 -0.24331738540692527 1103 52-53 -0.2362419324709748 1103 54-55 -0.18821114318090792 1103 56-57 -0.24050519964229267 1103 58-59 -0.26560515930569295 1103 60-61 -0.31479924336527176 1103 62-63 -0.20838542508635527 1103 64-65 -0.16758099261122794 1103 66-67 -0.03833614935000895 1103 68-69 -0.06678837015904548 1103 70-71 0.06308597468948207 1103 72-73 -0.1003018935666411 1103 74-75 -0.030118002894422347 1103 76-77 0.24596251246769185 1103 78-79 0.1704002460694447 1103 80-81 -0.027230935170496906 1103 82-83 0.08763627857720024 1103 84-85 -0.024851062109625843 1103 86-87 0.03524800352176882 1103 88-89 0.13118095640948013 1103 90-91 0.2523533459184826 1103 92-93 0.012569418402680554 1103 94-95 0.01794528074620061 1103 96-97 0.06437440507989578 1103 98-99 0.33618110666190404 1103 100-101 0.5498427729958308 1104 1 -0.09547953433115808 1104 2 0.04705108249399004 1104 3 -0.004733527054540332 1104 4 -0.14063463387009278 1104 5 -0.04079868113426954 1104 6 0.08476695543436108 1104 7 -0.009523404784154366 1104 8 0.0017337937429218186 1104 9 0.08062118480215474 1104 10-11 0.0038052529755319142 1104 12-13 0.19078387518317896 1104 14-15 0.08504452294373976 1104 16-17 0.06624896637880795 1104 18-19 0.018188089832875676 1104 20-21 0.13086742972044618 1104 22-23 0.22338442216135235 1104 24-25 0.13094209206057883 1104 26-27 0.12392509429287912 1104 28-29 0.0022037020294263243 1104 30-31 0.01138250762014792 1104 32-33 0.10248483720121015 1104 34-35 0.11545855997889731 1104 36-37 -0.17104976969108066 1104 38-39 -0.037275169460201596 1104 40-41 0.013694474804104573 1104 42-43 0.17313031426858316 1104 44-45 0.25867234772186265 1104 46-47 0.23841323706819395 1104 48-49 0.2464842520127135 1104 50-51 0.17247094036211053 1104 52-53 0.08948865250129501 1104 54-55 0.1326385794615561 1104 56-57 0.12091701417515566 1104 58-59 -0.02833637463599814 1104 60-61 0.15623366132458472 1104 62-63 0.24109642060199832 1104 64-65 0.16756663724252263 1104 66-67 0.13072083501710807 1104 68-69 0.21599226524185866 1104 70-71 0.16944805234959404 1104 72-73 0.28472546397243903 1104 74-75 0.47248461939503983 1104 76-77 0.2702749179139836 1104 78-79 0.2824848401188653 1104 80-81 0.3714252675526488 1104 82-83 0.35963035321159253 1104 84-85 0.4002273544309709 1104 86-87 0.24521270347134205 1104 88-89 0.25536149095309923 1104 90-91 0.4461112884298295 1104 92-93 0.4149616271772629 1104 94-95 0.48136459491665207 1104 96-97 0.5898586461288104 1104 98-99 0.6071957309685097 1104 100-101 0.5003088597330994 1105 1 0.08704794260024329 1105 2 0.1012359919729029 1105 3 0.12289853086826596 1105 4 0.11928077291539552 1105 5 0.11562362368851353 1105 6 -0.06421211500877888 1105 7 0.057076635959518285 1105 8 0.14404526241872162 1105 9 0.21342718814847927 1105 10-11 0.13250933702772016 1105 12-13 0.17273768454955274 1105 14-15 0.32611389405514046 1105 16-17 0.2931194181994172 1105 18-19 0.30104685957435606 1105 20-21 0.12112818922257418 1105 22-23 0.2407518298952951 1105 24-25 0.3644045861744516 1105 26-27 0.1845546842178365 1105 28-29 0.10853508500992604 1105 30-31 0.055499638998455225 1105 32-33 0.11112867405286408 1105 34-35 0.23191741721907988 1105 36-37 0.2512166471701178 1105 38-39 0.3549736808669195 1105 40-41 0.39230504878893413 1105 42-43 0.2426116339628024 1105 44-45 0.39289978758319677 1105 46-47 0.39515823511450066 1105 48-49 0.416751473728425 1105 50-51 0.27020524152464276 1105 52-53 0.3615753964473498 1105 54-55 0.3574179958919359 1105 56-57 0.3951962352362983 1105 58-59 0.4374610658598712 1105 60-61 0.6544077102771055 1105 62-63 0.6411675550460956 1105 64-65 0.6155762921569803 1105 66-67 0.6042466321069426 1105 68-69 0.7074699079866562 1105 70-71 0.6298210740272303 1105 72-73 0.6758388349101772 1105 74-75 0.5229195246994003 1105 76-77 0.7169812762645975 1105 78-79 0.8199615043475923 1105 80-81 0.6040223760877801 1105 82-83 0.6908851748244444 1105 84-85 0.7164303948440143 1105 86-87 0.7364290189743095 1105 88-89 0.818542987314558 1105 90-91 0.8463423083908879 1105 92-93 0.8070092638772941 1105 94-95 0.7006836913422276 1105 96-97 0.4734974735125661 1105 98-99 0.5975541750945723 1105 100-101 0.7346258855786125 1106 1 0.20013762579134564 1106 2 0.1644487932561809 1106 3 0.06518762788982002 1106 4 0.16723779964157615 1106 5 0.17811214578418344 1106 6 0.13597471716458642 1106 7 0.24834162282317607 1106 8 0.11116223772388878 1106 9 0.25681835138021114 1106 10-11 0.03859759227382398 1106 12-13 -0.0511156493834406 1106 14-15 -0.037639633643614445 1106 16-17 -0.07679329350936115 1106 18-19 -0.09532687223433811 1106 20-21 0.0035466633078087284 1106 22-23 0.1504619495653401 1106 24-25 -0.01837977539171476 1106 26-27 0.17529552906655255 1106 28-29 0.289819916935933 1106 30-31 -0.042408758892236165 1106 32-33 0.3329052100307095 1106 34-35 0.31320862055319765 1106 36-37 0.10162535426150043 1106 38-39 0.029240125658660077 1106 40-41 0.2354803337220588 1106 42-43 0.25068083495074944 1106 44-45 0.2619125378952063 1106 46-47 0.35845096009079924 1106 48-49 0.5331753710063296 1106 50-51 0.35886385398187315 1106 52-53 0.4109078977497447 1106 54-55 0.6309767458966249 1106 56-57 0.5879397635722015 1106 58-59 0.4734984060593277 1106 60-61 0.41327333727988247 1106 62-63 0.5846550389770258 1106 64-65 0.533233806483274 1106 66-67 0.49491795364942703 1106 68-69 0.5072048386694732 1106 70-71 0.25611596903017286 1106 72-73 0.5846461822681057 1106 74-75 0.7081892976149184 1106 76-77 0.47327632118302887 1106 78-79 0.44214543565631814 1106 80-81 0.5875269878628941 1106 82-83 0.42023291128801077 1106 84-85 0.3654684059152764 1106 86-87 0.49656096334067357 1106 88-89 0.43013398414009885 1106 90-91 0.5170833968522679 1106 92-93 0.6616886883517452 1106 94-95 0.749504137565669 1106 96-97 0.7343105963645584 1106 98-99 0.9796887467298134 1106 100-101 1.1173516864083943 1107 1 -0.014837701056439556 1107 2 -0.05525585390883592 1107 3 -0.0893227730836692 1107 4 -0.03351706663028153 1107 5 -0.01785183838224924 1107 6 0.1263729531849762 1107 7 -0.050488671951541164 1107 8 0.03927681889904733 1107 9 0.1399462652137018 1107 10-11 0.037933693700146875 1107 12-13 0.0844555955740347 1107 14-15 0.22251548331284 1107 16-17 -0.004348349904020665 1107 18-19 0.06704609407823625 1107 20-21 0.16721977083749806 1107 22-23 0.1402175367663645 1107 24-25 0.13795044829007708 1107 26-27 0.17104635944757973 1107 28-29 0.09101659059939493 1107 30-31 0.1754455971667781 1107 32-33 0.06952341163275122 1107 34-35 0.16541814188061466 1107 36-37 -0.00411592310498321 1107 38-39 -0.02401363359474118 1107 40-41 0.004138789858657788 1107 42-43 8.330988151996621E-4 1107 44-45 0.021313132325836648 1107 46-47 -0.05031525104988788 1107 48-49 -0.09679265748834354 1107 50-51 -0.1754459055615314 1107 52-53 0.010764555657132746 1107 54-55 0.13849509646569658 1107 56-57 0.005535247624415263 1107 58-59 -0.0036681079528477767 1107 60-61 -0.07169308654583517 1107 62-63 0.0032283242234214526 1107 64-65 -0.058477320606819205 1107 66-67 0.052817909181193556 1107 68-69 0.23614812901268323 1107 70-71 0.26643881842222683 1107 72-73 0.20347074585908942 1107 74-75 0.023960959005083993 1107 76-77 -0.06593947538432587 1107 78-79 0.20828470217104922 1107 80-81 0.2037322067400531 1107 82-83 0.298109635838383 1107 84-85 0.4479090151957301 1107 86-87 0.4395108417327016 1107 88-89 0.34363264056188214 1107 90-91 0.4233494806506748 1107 92-93 0.4980297994076466 1107 94-95 0.37475284562748 1107 96-97 0.505994642517777 1107 98-99 0.5916057339396517 1107 100-101 0.8365005587495595 1108 1 -0.06092155460140347 1108 2 -0.07517462416753062 1108 3 -0.20946807595941053 1108 4 -0.22533522672254946 1108 5 -0.05496149098612335 1108 6 0.05616104896513008 1108 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82-83 -0.3212036308665276 2315 84-85 -0.10131225227780405 2315 86-87 -0.3300042603850848 2315 88-89 -0.37755125575093373 2315 90-91 -0.41248235912679476 2315 92-93 -0.41966699815618114 2315 94-95 -0.24489365845172983 2315 96-97 -0.2715017268735309 2315 98-99 -0.512095736805108 2315 100-101 -0.26428821018657445 2316 1 -0.5805910776877852 2316 2 -0.7208670950308438 2316 3 -0.5615157793738561 2316 4 -0.35033974637399723 2316 5 -2.370632026474425 2316 6 -0.5801727303071402 2316 7 -0.4252195201776985 2316 8 -0.5607797139294348 2316 9 -0.4679123822639397 2316 10-11 -0.3972175279640453 2316 12-13 -0.4900658474592845 2316 14-15 -0.5862647807407555 2316 16-17 -0.6190752978032421 2316 18-19 -0.5340165624095974 2316 20-21 -0.4009906656777389 2316 22-23 -0.45354807549731646 2316 24-25 -0.330723133787707 2316 26-27 -0.5965781515507658 2316 28-29 -0.40819640523149303 2316 30-31 -0.3352813974779494 2316 32-33 -0.353249019629466 2316 34-35 -0.4787679606848414 2316 36-37 -0.8335620766814529 2316 38-39 -0.8181797232467574 2316 40-41 -0.8667746316483615 2316 42-43 -1.2092919015499959 2316 44-45 -1.4063924090599897 2316 46-47 -1.5057974746160383 2316 48-49 -1.5045623424529424 2316 50-51 -1.1238213536608939 2316 52-53 -1.1691982816773177 2316 54-55 -1.4357772807470468 2316 56-57 -1.6886758345629218 2316 58-59 -1.6048538365543692 2316 60-61 -1.5662026825187212 2316 62-63 -1.7440071182080494 2316 64-65 -1.7877489820292212 2316 66-67 -1.8595266255404823 2316 68-69 -1.3610635024341775 2316 70-71 -1.235310850707343 2316 72-73 -1.3589381369528866 2316 74-75 -1.7361338759102942 2316 76-77 -1.5755745245693475 2316 78-79 -1.523958219539022 2316 80-81 -1.694687284376844 2316 82-83 -1.6777989066079826 2316 84-85 -1.6566949774535438 2316 86-87 -1.960095911822144 2316 88-89 -1.709427509198985 2316 90-91 -1.7485672890021497 2316 92-93 -1.7310483064650448 2316 94-95 -1.9320732377723182 2316 96-97 -1.9496427906872995 2316 98-99 -2.1364326499518533 2316 100-101 -2.3318357984327385 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7232.0 3 1565.0 4 1585.0 5 1730.0 6 1919.0 7 2272.0 8 2827.0 9 3519.0 10 4216.0 11 5275.0 12 6364.0 13 7809.0 14 8788.0 15 10133.0 16 12078.0 17 14811.0 18 13237.0 19 10705.0 20 10877.0 21 11609.0 22 12749.0 23 14157.0 24 15750.0 25 18050.0 26 20652.0 27 24102.0 28 28299.0 29 33634.0 30 40888.0 31 50327.0 32 62017.0 33 79492.0 34 104926.0 35 145016.0 36 206244.0 37 278139.0 38 219608.0 39 28860.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.80025356585225 19.102334666027254 15.066389529656451 23.03102223846404 2 14.834415252588858 19.87887339731438 40.72306713394149 24.56364421615527 3 19.126852301822744 24.11775605169334 29.825084286203175 26.930307360280743 4 12.310215288809056 16.358032628628287 37.81424721451269 33.51750486804996 5 13.450959398305889 37.43591704553845 34.56194158166225 14.551181974493401 6 31.631721379295808 36.40038946186805 17.563794802607653 14.40409435622849 7 28.201514780175035 32.467170539264906 20.864331248797637 18.466983431762422 8 26.047128783581414 35.04464565129022 19.676566913135172 19.231658651993193 9 25.694890957194506 16.932663827723964 20.508835682136798 36.863609532944736 10-11 24.701502198729287 26.582536597996388 27.64294705162081 21.073014151653517 12-13 25.689784271630568 23.790701282735043 28.18696524568433 22.332549199950062 14-15 22.330177667582713 25.4549388847756 26.598955547533915 25.615927900107767 16-17 21.72707969196044 28.76189917759051 27.160760203365662 22.350260927083383 18-19 21.769668009804445 27.549277478826973 29.204156283533155 21.476898227835427 20-21 22.513207132032452 26.402143048415883 29.25769643375321 21.82695338579845 22-23 22.100327160227486 26.196894925673398 29.339550025410666 22.36322788868845 24-25 22.080647450715503 26.506973222575013 29.32231283022236 22.090066496487122 26-27 21.866864123617887 26.546409410475057 29.473908540891507 22.112817925015545 28-29 21.603409634410983 27.10760151627146 29.39037533911224 21.89861351020532 30-31 21.896299654033495 26.527447708062514 29.246918354936202 22.32933428296779 32-33 21.757555672247452 26.633271114736317 29.225665072438655 22.38350814057757 34-35 22.287484304820477 26.603741019326975 29.004230616700326 22.104544059152218 36-37 22.087825366356224 26.473988477383603 29.26584491501642 22.17234124124375 38-39 21.804083648937922 26.791832702124157 29.253515560678412 22.150568088259508 40-41 21.916606399753398 26.85925074065731 29.11178425630629 22.112358603283 42-43 21.86074497378335 26.634806873468804 28.873126952746137 22.631321200001715 44-45 22.068170191936805 26.658921426437406 28.730207721545547 22.542700660080246 46-47 22.276588891150507 27.118900300180428 28.4020942146267 22.202416594042372 48-49 22.201374974101064 26.887097767701118 28.462609512559787 22.448917745638024 50-51 21.979828407052942 27.187704657729917 28.580003136163523 22.25246379905362 52-53 22.26157816411834 27.49944732924121 27.874472738917678 22.364501767722775 54-55 22.090084664601473 27.114251254122617 28.13787623266738 22.657787848608532 56-57 22.168907119426816 27.34396276438973 28.071603599082284 22.415526517101174 58-59 22.508748223685856 27.00290531899902 28.11133192940312 22.377014527912003 60-61 22.237010820995536 27.08805172687441 28.62664042037964 22.048297031750412 62-63 21.869238401229712 27.35947502789935 28.473967246540692 22.297319324330243 64-65 21.83406588051205 27.44727175019724 28.270778150819982 22.447884218470726 66-67 21.911822628301646 27.36409095160375 27.952363827491773 22.77172259260284 68-69 21.80526294645709 27.232655899822646 28.239750742050816 22.722330411669446 70-71 22.16732806388929 27.259312825244596 27.996580558247576 22.57677855261854 72-73 22.10767230820053 27.02903698697497 28.286352388866327 22.576938315958174 74-75 21.763467064044196 27.70789458518232 27.941183734964557 22.58745461580893 76-77 21.99036082832453 27.276886243875058 27.885320667304974 22.847432260495438 78-79 22.12177252419526 27.322588666555784 27.944345546375693 22.611293262873264 80-81 22.18600259951441 27.420760748188588 27.787735521307354 22.605501130989644 82-83 22.12019464319531 27.294278445159858 28.08480646834578 22.500720443299056 84-85 22.175043481639587 27.246211120894436 27.95571425466773 22.623031142798247 86-87 21.957448938462715 27.367087207020184 27.954627480630467 22.72083637388663 88-89 21.983207768136257 27.256484973672805 27.79155869904005 22.96874855915089 90-91 21.996861533698993 27.090233376090683 27.98408702081169 22.928818069398638 92-93 22.25557714202532 26.909823483129674 28.202980733224937 22.63161864162007 94-95 22.01424372411635 26.97731707517887 28.08272886700643 22.925710333698348 96-97 21.611187713168963 27.128808144062948 28.339958578905406 22.92004556386268 98-99 21.77995366093439 27.168129788905297 27.92289337568731 23.129023174473005 100-101 21.764092459534385 26.730570461721438 28.35538553178655 23.149951546957634 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2250.0 1 1319.5 2 377.5 3 353.5 4 287.5 5 289.0 6 484.5 7 983.5 8 1577.5 9 1883.5 10 1749.0 11 1533.0 12 1554.5 13 1658.0 14 1773.0 15 1868.5 16 1914.0 17 2025.0 18 2215.0 19 2439.0 20 2728.0 21 3197.0 22 3762.5 23 4445.5 24 5536.0 25 6970.5 26 8943.0 27 11457.5 28 13455.5 29 15623.0 30 18455.5 31 20751.0 32 23196.0 33 26087.0 34 29294.0 35 32038.5 36 35058.5 37 38398.0 38 40489.5 39 41982.0 40 43794.0 41 46689.0 42 49388.5 43 51909.5 44 54827.5 45 57169.0 46 58563.0 47 62064.5 48 68615.5 49 67782.5 50 60250.0 51 55441.5 52 52734.5 53 50750.0 54 48997.5 55 45461.5 56 40107.0 57 35579.5 58 31798.5 59 27595.0 60 23160.0 61 19118.5 62 15012.5 63 11258.0 64 8626.5 65 6552.0 66 4923.0 67 3801.5 68 2873.0 69 2023.5 70 1469.5 71 1056.5 72 802.5 73 604.5 74 438.5 75 332.5 76 218.5 77 136.5 78 82.5 79 51.5 80 29.0 81 14.5 82 11.0 83 9.5 84 12.0 85 10.0 86 7.0 87 7.0 88 5.5 89 5.0 90 4.0 91 2.5 92 2.5 93 2.0 94 1.0 95 1.0 96 1.0 97 2.0 98 2.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3608359563500227 2 0.32021726399586714 3 0.3225834014145558 4 0.32508099091206055 5 0.3077950425477516 6 0.228397986942865 7 0.23812544077525177 8 0.23832261889347583 9 0.23207864514971405 10-11 0.24045871517423645 12-13 0.24026153705601236 14-15 0.2202479580562707 16-17 0.23727100226294753 18-19 0.22215401319910325 20-21 0.2262618906621044 22-23 0.2236985751251917 24-25 0.2146941077262931 26-27 0.22997541188865744 28-29 0.2322100972285301 30-31 0.242529085415589 32-33 0.24318634580966925 34-35 0.2290552473369452 36-37 0.22406006834193576 38-39 0.21144066877559617 40-41 0.21170357293322828 42-43 0.21377394317458084 44-45 0.23303167272113032 46-47 0.22215401319910325 48-49 0.23316312479994636 50-51 0.242463359376181 52-53 0.2521579501888638 54-55 0.24795148366675057 56-57 0.20608399656384266 58-59 0.18883091121923776 60-61 0.19914989940629668 62-63 0.2012859956870573 64-65 0.1985583650516245 66-67 0.21091486046033203 68-69 0.19980715980037686 70-71 0.20312632479048184 72-73 0.2202808210759747 74-75 0.20897594229779548 76-77 0.22284413661288743 78-79 0.22014936899715865 80-81 0.20417794142101012 82-83 0.21719169722379783 84-85 0.1978353786181363 86-87 0.20394790028308205 88-89 0.2145955186671811 90-91 0.21094772348003604 92-93 0.21581145039622943 94-95 0.21390539525339688 96-97 0.20759569547022708 98-99 0.21752032742083793 100-101 0.1962908166920479 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1521467.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.64699928647709 #Duplication Level Percentage of deduplicated Percentage of total 1 78.86728817688386 47.04197081612974 2 12.07419168736792 14.403786059224439 3 3.8853640916872565 6.952509276137205 4 1.6959586761536927 4.046353837857358 5 0.9256024543157555 2.7604704466066656 6 0.5520219980726137 1.9755873435094107 7 0.39116262175074085 1.6332173634324063 8 0.2546744266654276 1.215245227647738 9 0.19483590739130183 1.0459239506234186 >10 1.068067000113513 11.414820348120102 >50 0.06315275288279236 2.556355867898831 >100 0.024923349443336348 2.4366042970348256 >500 0.0014335657813341325 0.5778360587252912 >1k 0.00121301719959042 1.5830142772158888 >5k 1.1027429087185635E-4 0.35630482983664225 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5417 0.35603795547323736 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4120 0.27079128236103706 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3806 0.2501533059869192 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2956 0.1942861724901033 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2628 0.17272803156427316 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2425 0.15938564556444537 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1956 0.12856013308208458 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1584 0.10411004642230162 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.57260394080187E-5 0.0 12-13 0.0 0.0 0.0 6.57260394080187E-5 0.0 14-15 0.0 0.0 0.0 6.57260394080187E-5 0.0 16-17 0.0 0.0 0.0 1.314520788160374E-4 0.0 18-19 0.0 0.0 0.0 1.9717811822405612E-4 0.0 20-21 0.0 0.0 0.0 2.629041576320748E-4 3.286301970400935E-5 22-23 0.0 0.0 0.0 3.2863019704009355E-4 6.57260394080187E-5 24-25 0.0 0.0 0.0 4.6008227585613096E-4 6.57260394080187E-5 26-27 0.0 0.0 0.0 6.572603940801871E-4 6.57260394080187E-5 28-29 0.0 0.0 0.0 0.0011830687093443368 6.57260394080187E-5 30-31 0.0 0.0 0.0 0.00253045251720872 6.57260394080187E-5 32-33 0.0 0.0 0.0 0.004666548797969329 6.57260394080187E-5 34-35 0.0 0.0 0.0 0.0063754258225778146 6.57260394080187E-5 36-37 0.0 0.0 0.0 0.007361316413698095 6.57260394080187E-5 38-39 0.0 0.0 0.0 0.008478659083634415 6.57260394080187E-5 40-41 0.0 0.0 0.0 0.009497412694458703 6.57260394080187E-5 42-43 0.0 0.0 0.0 0.01169923501462733 9.858905911202806E-5 44-45 0.0 0.0 0.0 0.013605290157459872 1.314520788160374E-4 46-47 0.0 0.0 0.0 0.016562961930820713 1.314520788160374E-4 48-49 0.0 0.0 0.0 0.020079305039149717 1.314520788160374E-4 50-51 0.0 0.0 0.0 0.024220045521854895 1.314520788160374E-4 52-53 0.0 0.0 0.0 0.041013048590603673 1.6431509852004678E-4 54-55 0.0 0.0 0.0 0.0600736000189291 1.9717811822405612E-4 56-57 0.0 0.0 0.0 0.08169746698416726 1.9717811822405612E-4 58-59 0.0 0.0 0.0 0.11318023986060821 1.9717811822405612E-4 60-61 0.0 0.0 0.0 0.1612259746678699 1.9717811822405612E-4 62-63 0.0 0.0 0.0 0.1911641856182224 1.9717811822405612E-4 64-65 0.0 0.0 0.0 0.22175965696265512 2.629041576320748E-4 66-67 0.0 0.0 0.0 0.29014760096669856 3.2863019704009355E-4 68-69 0.0 0.0 0.0 0.3769059729852833 3.2863019704009355E-4 70-71 0.0 0.0 0.0 0.44585258832429486 3.2863019704009355E-4 72-73 0.0 0.0 0.0 0.5190385332051237 3.2863019704009355E-4 74-75 0.0 0.0 0.0 0.660612422089996 3.9435623644811223E-4 76-77 0.0 0.0 0.0 0.7580841385320878 3.9435623644811223E-4 78-79 0.0 0.0 0.0 0.8404388659103352 4.272192561521216E-4 80-81 0.0 0.0 0.0 0.9591400930812171 4.6008227585613096E-4 82-83 0.0 0.0 0.0 1.1515530734481918 4.6008227585613096E-4 84-85 0.0 0.0 0.0 1.334764408298044 4.6008227585613096E-4 86-87 0.0 0.0 0.0 1.5796267681126177 4.6008227585613096E-4 88-89 0.0 0.0 0.0 1.9164069940393054 4.6008227585613096E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGCGG 60 0.003932675 31.69732 5 GTACATG 5480 0.0 26.209265 1 TACATGG 5450 0.0 25.821434 2 ACATGGG 5320 0.0 24.396181 3 GTATCAA 9715 0.0 23.399876 1 GAGTACT 4865 0.0 23.139658 12-13 GCGTATC 105 0.0021808783 22.64692 1 GTACTTT 5180 0.0 21.548054 14-15 ACCGGCG 155 3.8784325E-5 21.472378 5 GTCTACG 135 3.7652755E-4 21.137125 1 AGTACTT 5145 0.0 20.957134 12-13 AGAGTAC 7960 0.0 20.855738 10-11 TCGATTG 70 4.8609014E-4 20.353018 84-85 CAACGCA 11145 0.0 20.349422 5 TCAACGC 11150 0.0 20.3403 4 ATCAACG 11190 0.0 20.223099 3 AACGCAG 11365 0.0 19.97889 6 GGTATCA 7520 0.0 19.92146 1 TATCAAC 11425 0.0 19.89101 2 TCTACGG 145 6.110069E-4 19.6729 2 >>END_MODULE