FastQCFastQC Report
Thu 26 May 2016
SRR937373_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937373_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1521467
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63010.4141397743099259No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53170.34946535153243546No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT50210.33001044386766193No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36640.24082020839098056No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19110.12560246130872377No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT17860.11738670638272142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG54950.022.4025941
TACATGG55450.022.188912
ACATGGG54800.021.9319743
GAGTACT60750.021.3059512-13
AGTACTT62350.021.02583912-13
GTATCAA118750.020.5729121
AGAGTAC93050.020.46956810-11
GTACTTT63400.020.30235714-15
ACGATCG600.0047660919.79107982-83
GGTATCA95250.018.113731
CATGGGG30800.018.0456664
TCAACGC136600.017.7708424
ATCAACG136950.017.7254243
CAACGCA137000.017.7189565
TACTTTT73400.017.63343214-15
TATCAAC139350.017.4883232
GTCTAGC3007.629751E-817.4276031
TAAGGTG6550.017.4063515
AACGCAG139450.017.404796
ACTTTTT75250.017.26332316-17
Produced by FastQC (version 0.11.5)