##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937373_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1521467 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13184906409406 33.0 31.0 34.0 31.0 34.0 2 32.35524858573995 34.0 31.0 34.0 31.0 34.0 3 32.43923594793709 34.0 31.0 34.0 31.0 34.0 4 36.05779093467029 37.0 35.0 37.0 35.0 37.0 5 35.939942831490924 37.0 35.0 37.0 35.0 37.0 6 36.017964898351394 37.0 35.0 37.0 35.0 37.0 7 35.971383539702146 37.0 35.0 37.0 35.0 37.0 8 35.94846552702096 37.0 35.0 37.0 35.0 37.0 9 37.64104249385626 39.0 37.0 39.0 35.0 39.0 10-11 37.56065658998848 39.0 37.0 39.0 35.0 39.0 12-13 37.48274034205146 39.0 37.0 39.0 35.0 39.0 14-15 38.68496852051342 40.0 38.0 41.0 34.0 41.0 16-17 38.66333939546503 40.0 38.0 41.0 34.0 41.0 18-19 38.653083832906006 40.0 38.0 41.0 34.0 41.0 20-21 38.537860827740595 40.0 38.0 41.0 34.0 41.0 22-23 38.416765200954075 40.0 38.0 41.0 34.0 41.0 24-25 38.27605528085722 40.0 38.0 41.0 34.0 41.0 26-27 38.23330016359211 40.0 38.0 41.0 34.0 41.0 28-29 38.14338365537998 40.0 38.0 41.0 33.5 41.0 30-31 38.24396519937665 40.0 38.0 41.0 34.0 41.0 32-33 38.187801312811914 40.0 38.0 41.0 34.0 41.0 34-35 38.07872270644056 40.0 37.5 41.0 33.5 41.0 36-37 37.93096629765877 40.0 37.0 41.0 33.0 41.0 38-39 37.807676407046614 40.0 37.0 41.0 33.0 41.0 40-41 37.660168442693795 40.0 37.0 41.0 33.0 41.0 42-43 37.47044102829703 40.0 37.0 41.0 32.5 41.0 44-45 37.20398667864633 40.0 36.5 41.0 31.5 41.0 46-47 36.89510058384441 40.0 36.0 41.0 31.0 41.0 48-49 36.61870385621246 39.0 36.0 41.0 30.5 41.0 50-51 36.409148867507476 39.0 36.0 41.0 30.0 41.0 52-53 36.193294366555435 39.0 35.0 40.5 30.0 41.0 54-55 35.96763124011234 39.0 35.0 40.0 29.0 41.0 56-57 35.70744485420978 38.5 35.0 40.0 28.5 41.0 58-59 35.461176943042474 38.0 35.0 40.0 28.0 41.0 60-61 35.11711525783997 38.0 34.0 40.0 28.0 41.0 62-63 34.756331225061075 37.5 34.0 40.0 27.0 41.0 64-65 34.40667263897278 37.0 34.0 40.0 26.0 41.0 66-67 33.96739889856303 36.5 33.0 39.0 26.0 40.5 68-69 33.55678664078813 36.0 33.0 39.0 26.0 40.0 70-71 33.13285237208562 35.5 32.5 38.5 25.0 40.0 72-73 32.67479215783188 35.0 32.0 38.0 24.0 40.0 74-75 32.21849471595506 35.0 31.5 37.0 23.0 39.0 76-77 30.907629938736758 34.0 30.0 35.5 21.5 38.5 78-79 31.56525511233566 34.5 31.0 36.0 23.0 38.5 80-81 31.41253080086522 34.5 31.0 36.0 22.0 37.5 82-83 31.077908360812295 34.0 31.0 35.0 20.5 37.0 84-85 30.701737862207985 34.0 31.0 35.0 20.0 36.5 86-87 30.326619637494602 34.0 30.5 35.0 18.0 36.0 88-89 29.924789693105403 34.0 30.0 35.0 16.0 36.0 90-91 29.459866694446873 34.0 29.5 35.0 8.5 35.0 92-93 29.074907638483122 34.0 29.0 35.0 4.5 35.0 94-95 28.62441380588603 34.0 29.0 35.0 2.0 35.0 96-97 28.117918758671728 33.0 29.0 35.0 2.0 35.0 98-99 27.587278593620496 33.0 28.0 35.0 2.0 35.0 100-101 25.639606708525392 31.5 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11537184409623791 1101 2 0.1631723793167552 1101 3 0.12368328791003336 1101 4 0.1042125174893016 1101 5 -3.5898021165792215E-4 1101 6 -0.020585331912919003 1101 7 -0.016394830234276014 1101 8 -0.0470490829711494 1101 9 0.06429334782078655 1101 10-11 0.00949761658796433 1101 12-13 0.06025747220836308 1101 14-15 0.0448180733493615 1101 16-17 0.057266132711433215 1101 18-19 0.04116328307878803 1101 20-21 0.05769219611890719 1101 22-23 -0.0032296577520085634 1101 24-25 0.05004414219366993 1101 26-27 0.04977476394826397 1101 28-29 -0.08004476193720222 1101 30-31 0.007420606542844155 1101 32-33 0.08062457944475909 1101 34-35 0.04903184317841891 1101 36-37 0.14590114781598373 1101 38-39 -0.08500242248118894 1101 40-41 -0.024304155794702353 1101 42-43 -0.007615347581456433 1101 44-45 -0.03981396456079267 1101 46-47 -0.1942925847092951 1101 48-49 -0.15266273909945483 1101 50-51 -0.21896407254045158 1101 52-53 -0.12039378586229788 1101 54-55 -0.21201550041991624 1101 56-57 -0.1485468274647488 1101 58-59 -0.13776203725058878 1101 60-61 -0.20050293180668888 1101 62-63 -0.13641919072318842 1101 64-65 -0.1346275730240265 1101 66-67 -0.13862917642470762 1101 68-69 0.04852017414466303 1101 70-71 -0.07986398578565712 1101 72-73 -0.14590257395741446 1101 74-75 -0.1777120715156002 1101 76-77 -0.18384383500211143 1101 78-79 -0.20724386511162862 1101 80-81 -0.06058787422553635 1101 82-83 -0.0049564252995999425 1101 84-85 -0.038635336117231134 1101 86-87 0.0371711083509112 1101 88-89 -0.11646201765111641 1101 90-91 -0.08792352491324706 1101 92-93 -0.07649426841357965 1101 94-95 -0.04145333566700771 1101 96-97 0.17622379457883497 1101 98-99 0.2766889011627214 1101 100-101 0.5437127221983467 1102 1 0.09598320374008296 1102 2 0.11879848111946245 1102 3 0.08435671384367538 1102 4 0.08030269113289279 1102 5 -0.059656677141688874 1102 6 0.006294795039458734 1102 7 0.003914101610128284 1102 8 -0.022145640252546173 1102 9 0.11566372539500236 1102 10-11 0.058148155898834375 1102 12-13 0.08609754371169487 1102 14-15 0.09617635875137864 1102 16-17 -0.04147700176484648 1102 18-19 0.0174839798348998 1102 20-21 0.09111676103225363 1102 22-23 0.11547906806236341 1102 24-25 0.025792133092117808 1102 26-27 -0.07815720188903441 1102 28-29 -0.04838822696715539 1102 30-31 0.20202044826402954 1102 32-33 0.16822220443921765 1102 34-35 0.09283946752454142 1102 36-37 0.10429134008666807 1102 38-39 0.06550884485049835 1102 40-41 0.10697579805950852 1102 42-43 -0.012154800480139727 1102 44-45 0.1302476437701472 1102 46-47 0.2455298198731768 1102 48-49 0.323538708256379 1102 50-51 0.39784450991735554 1102 52-53 0.2781681975832626 1102 54-55 0.33528573257000005 1102 56-57 0.269807340424002 1102 58-59 0.3205185536295474 1102 60-61 0.20752040454197385 1102 62-63 0.07500382124651139 1102 64-65 0.23189079730106954 1102 66-67 0.23622661798984979 1102 68-69 0.297976155035947 1102 70-71 0.28041713920830347 1102 72-73 0.23137114033594486 1102 74-75 0.39986744609419844 1102 76-77 0.4098779606494425 1102 78-79 0.32199616502103723 1102 80-81 0.3436508695058258 1102 82-83 0.33654298347036615 1102 84-85 0.2972450717851842 1102 86-87 0.35012990337367 1102 88-89 0.32863154488805435 1102 90-91 0.28562258434029886 1102 92-93 0.22980071060778684 1102 94-95 0.3355412295447877 1102 96-97 0.31146817995173137 1102 98-99 0.35382422440449446 1102 100-101 0.6518899378702159 1103 1 0.06497306175224793 1103 2 0.11884513426348065 1103 3 0.056679229056655345 1103 4 0.09753494265419249 1103 5 -0.06141732622890572 1103 6 -0.04797498183680915 1103 7 -0.029181233075469493 1103 8 0.04953995812473977 1103 9 -0.05632207582203819 1103 10-11 -0.03088886235673982 1103 12-13 0.11187847677457086 1103 14-15 0.1268568861347461 1103 16-17 0.16330494549681873 1103 18-19 0.07657728612292658 1103 20-21 0.02818420525132126 1103 22-23 -0.04087235181593485 1103 24-25 -0.01941780605595511 1103 26-27 0.08868813279656962 1103 28-29 -0.10360780100366185 1103 30-31 -0.049191519281613694 1103 32-33 0.07039106853658694 1103 34-35 -0.029582946268561727 1103 36-37 0.036360305603906795 1103 38-39 0.0767167556010051 1103 40-41 0.055514844712661215 1103 42-43 0.16083353623385932 1103 44-45 6.776640541232837E-4 1103 46-47 0.15923925192186061 1103 48-49 0.25584144659308805 1103 50-51 0.23006053425812922 1103 52-53 0.33483756878001714 1103 54-55 0.27801595569373205 1103 56-57 0.2073722491461112 1103 58-59 0.2455631783759955 1103 60-61 0.12001634774683367 1103 62-63 0.1660104135386007 1103 64-65 0.12059870805157402 1103 66-67 0.23374740906489677 1103 68-69 0.22471449174993552 1103 70-71 0.15851450229146735 1103 72-73 0.2603620125468993 1103 74-75 0.23309665501915333 1103 76-77 0.2907871898583636 1103 78-79 0.21409555650176415 1103 80-81 0.32346476402915414 1103 82-83 0.25387766906874276 1103 84-85 0.33127904744441494 1103 86-87 0.19666489323168435 1103 88-89 0.16429330959393695 1103 90-91 0.23750392308269497 1103 92-93 0.17995436172340717 1103 94-95 0.1258292619991508 1103 96-97 0.21765174993144853 1103 98-99 0.3377963339379626 1103 100-101 0.7031820271197056 1104 1 0.05797760033016175 1104 2 0.07388144288677978 1104 3 0.06134135714540889 1104 4 -0.020750735611066773 1104 5 -0.03324889455971913 1104 6 -0.0028892531429178803 1104 7 -0.09779141966145488 1104 8 0.021188470479756916 1104 9 -0.061461258878011904 1104 10-11 0.018961238500551758 1104 12-13 -0.04010589034594858 1104 14-15 -0.04603292727927055 1104 16-17 -0.1122535517296086 1104 18-19 -0.10727193223308973 1104 20-21 -0.25675112001342626 1104 22-23 -0.129239094125559 1104 24-25 -0.042189616444247235 1104 26-27 -0.045728029583663954 1104 28-29 -0.191935713257827 1104 30-31 -0.0024076060188775728 1104 32-33 0.05687783101767252 1104 34-35 0.03978505675716093 1104 36-37 -0.030677011592260328 1104 38-39 -0.14037376381401856 1104 40-41 -0.07568649164639396 1104 42-43 -0.025730129928518863 1104 44-45 0.0942444820067152 1104 46-47 0.1695285862637661 1104 48-49 0.0320730401382221 1104 50-51 -0.1661863139516484 1104 52-53 -0.06967730766980651 1104 54-55 0.008779445355855842 1104 56-57 0.15827769543960812 1104 58-59 0.1382096735852727 1104 60-61 0.027668642250112896 1104 62-63 -0.07786439735398432 1104 64-65 -0.011435079139552329 1104 66-67 0.08002312767306563 1104 68-69 0.10254253007570924 1104 70-71 -0.15440128187393753 1104 72-73 -0.024467790781393717 1104 74-75 0.013836947505289743 1104 76-77 -0.006167298290900902 1104 78-79 -0.04489587062884581 1104 80-81 0.014755359087146758 1104 82-83 0.0022256267086824266 1104 84-85 0.06236011148878973 1104 86-87 0.12383622454787258 1104 88-89 0.11585974933171173 1104 90-91 0.21469630835752795 1104 92-93 0.12026033751766008 1104 94-95 0.11926244404655506 1104 96-97 0.0828920424175763 1104 98-99 0.13540311659050985 1104 100-101 0.4667614522331718 1105 1 -0.043419874230089306 1105 2 0.059611138678491216 1105 3 0.023114107643849024 1105 4 -0.04265379906766498 1105 5 0.06458267377109195 1105 6 0.0526983073464109 1105 7 -0.013538848969510298 1105 8 -0.08977792266996687 1105 9 -0.022394923504158726 1105 10-11 -0.024320981562041766 1105 12-13 -0.08935772631593153 1105 14-15 -0.03508682247452555 1105 16-17 -0.02677783596013228 1105 18-19 0.045632473760903736 1105 20-21 0.050956280306508006 1105 22-23 0.009803696749187907 1105 24-25 0.06172678555552835 1105 26-27 0.03421848599744948 1105 28-29 -0.1285233639175729 1105 30-31 -0.006316243343420069 1105 32-33 -0.021781668770259444 1105 34-35 -0.13407687562838788 1105 36-37 -0.2066529394292118 1105 38-39 -0.15895763733497148 1105 40-41 -0.0960850449120656 1105 42-43 -0.16278290968225662 1105 44-45 -0.11126935581342678 1105 46-47 -0.048009644325382794 1105 48-49 0.03331330089772422 1105 50-51 0.0017327196885972285 1105 52-53 0.058517480479792994 1105 54-55 0.13417268858556497 1105 56-57 -0.02112554334836858 1105 58-59 -0.033436821624000856 1105 60-61 -0.017777250487156948 1105 62-63 0.2417471685054835 1105 64-65 0.1241461694202215 1105 66-67 0.13963537815981653 1105 68-69 0.13934583832831038 1105 70-71 0.10588315571309437 1105 72-73 0.057784749853745154 1105 74-75 -0.002989437696079733 1105 76-77 0.027369419438937115 1105 78-79 0.03191012603818777 1105 80-81 0.07641564703136439 1105 82-83 0.14545382800914197 1105 84-85 -0.02049523511629303 1105 86-87 0.0724336570285935 1105 88-89 0.1334296230597225 1105 90-91 0.12061098710035267 1105 92-93 -0.013954578673942564 1105 94-95 -0.040014005109018314 1105 96-97 -0.12295200282793672 1105 98-99 0.03627701826466989 1105 100-101 0.5424712103426614 1106 1 -0.22032857944752493 1106 2 -0.033722494485807886 1106 3 -4.7696788731599327E-4 1106 4 0.06099589341819467 1106 5 -0.0802956510732784 1106 6 -0.16577634007110476 1106 7 -0.08659266488078998 1106 8 -0.05064623315992378 1106 9 -0.0922920814813466 1106 10-11 -0.25424214475629014 1106 12-13 -0.32522678638331826 1106 14-15 -0.24964820724386527 1106 16-17 -0.10448933860052279 1106 18-19 -0.11254382310174549 1106 20-21 -0.0576453080480519 1106 22-23 0.009449521415533013 1106 24-25 0.08954172988066489 1106 26-27 -0.11548009018023464 1106 28-29 0.09055844121478174 1106 30-31 0.03533168388759833 1106 32-33 -0.1726144492902364 1106 34-35 -0.18683775668169034 1106 36-37 -0.06059215619575298 1106 38-39 -0.01786983183531987 1106 40-41 -0.08703764538354619 1106 42-43 -0.429139157597497 1106 44-45 -0.2974508022729907 1106 46-47 -0.03429003500513517 1106 48-49 -0.10972434288059674 1106 50-51 -0.22246776228968912 1106 52-53 -0.13154443461556298 1106 54-55 -0.35418777945058366 1106 56-57 -0.29334676613402166 1106 58-59 -0.28817649338405005 1106 60-61 -0.09546158094585877 1106 62-63 -0.07696284622370797 1106 64-65 -0.07630395182392391 1106 66-67 -0.16267435098038163 1106 68-69 -0.05201612511480391 1106 70-71 -0.04889240206620826 1106 72-73 -0.030276074606469194 1106 74-75 -0.12610425047036955 1106 76-77 0.06026837265406115 1106 78-79 -0.048168167323925815 1106 80-81 -0.019781840385103777 1106 82-83 -0.2690278204615346 1106 84-85 -0.4368569174678676 1106 86-87 -0.16433864383679264 1106 88-89 -0.2162351284366224 1106 90-91 -0.35058181545720757 1106 92-93 -0.4453649648187543 1106 94-95 -0.8535018076103569 1106 96-97 -0.5038516456542936 1106 98-99 -0.3241118437643564 1106 100-101 0.3018362433053312 1107 1 -0.05607829109148099 1107 2 0.016762859277285713 1107 3 -0.0025891309654326733 1107 4 0.05618844900427433 1107 5 -0.08064095958064854 1107 6 0.04113876747295819 1107 7 0.04441457034805296 1107 8 -0.07384877738050477 1107 9 -0.0816931415315878 1107 10-11 -0.05881721685701535 1107 12-13 -0.09162511240267435 1107 14-15 -0.10485956886249426 1107 16-17 -0.05513928421052583 1107 18-19 -0.1254332053182594 1107 20-21 -0.010015546267169384 1107 22-23 0.032950812181859135 1107 24-25 -0.06398461997091687 1107 26-27 -0.05922917808140227 1107 28-29 -0.05451738134601669 1107 30-31 -0.16493834424019127 1107 32-33 -0.2569251931972616 1107 34-35 -0.12969904234807217 1107 36-37 -0.31587326700790896 1107 38-39 -0.1685557457794431 1107 40-41 -0.1700529355192586 1107 42-43 -0.20375660733322576 1107 44-45 -0.20731184450468731 1107 46-47 -0.24965707607372423 1107 48-49 -0.2228342850468863 1107 50-51 -0.301484192412218 1107 52-53 -0.19343465650987923 1107 54-55 -0.31222810835927817 1107 56-57 -0.2673248795120884 1107 58-59 -0.15912704801825583 1107 60-61 -0.1555129178522776 1107 62-63 -0.13900822257404144 1107 64-65 -0.09314563209199633 1107 66-67 -0.17534936067557538 1107 68-69 -0.22638379648860507 1107 70-71 -0.27500510013813795 1107 72-73 -0.145296463433219 1107 74-75 -0.2527476299063025 1107 76-77 -0.1311662888826639 1107 78-79 -0.2295046643706833 1107 80-81 -0.23999616915692812 1107 82-83 -0.1804911879163491 1107 84-85 -0.12117884874554008 1107 86-87 -0.1313871498384458 1107 88-89 -0.058991477816331184 1107 90-91 -0.115956872058117 1107 92-93 -0.01917333015952849 1107 94-95 -0.1437864199445258 1107 96-97 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-0.4972857673704034 2316 86-87 -0.32329674697995614 2316 88-89 -0.39460219305156485 2316 90-91 -0.5131971351183644 2316 92-93 -0.46160516208611924 2316 94-95 -0.49705962688974026 2316 96-97 -0.5457900490103498 2316 98-99 -0.4001520787604491 2316 100-101 -0.7214264384887628 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 11.0 5 28.0 6 47.0 7 142.0 8 288.0 9 628.0 10 1086.0 11 1793.0 12 2706.0 13 3811.0 14 5123.0 15 6843.0 16 9078.0 17 12751.0 18 11308.0 19 7402.0 20 6589.0 21 6952.0 22 7174.0 23 7825.0 24 9333.0 25 11232.0 26 13886.0 27 17449.0 28 21863.0 29 27110.0 30 34605.0 31 43437.0 32 55883.0 33 74040.0 34 101271.0 35 145173.0 36 222528.0 37 341111.0 38 283111.0 39 27841.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.57061574721956 19.900646050568714 15.455628741884912 22.073109460326812 2 15.696495553304803 20.847379535671823 39.63779694203029 23.818327968993085 3 19.592932347530375 24.456527811644946 30.17508759637902 25.775452244445656 4 13.207779071120177 17.249404686398062 37.202055647608525 32.34076059487324 5 13.51968051013197 37.81353230233208 34.400644238828534 14.266142948707413 6 31.829017652042403 37.04924260598489 17.016997411051307 14.104742330921408 7 28.54389875035081 32.87373304843286 20.545236932513163 18.037131268703167 8 26.38006608096002 35.51611701075344 19.17852966906282 18.925287239223724 9 25.890078457173242 17.123210690734666 20.331693030476508 36.65501782161559 10-11 24.97283871421464 27.026645993636407 27.27528760071694 20.725227691432018 12-13 26.050073023981575 24.01867862743835 27.97364038022424 21.957607968355834 14-15 22.512022935758715 25.803320085154656 26.465674247288966 25.218982731797666 16-17 21.842866128545673 29.03138878464009 27.100094842674867 22.02565024413937 18-19 21.887165479106677 27.706910501509398 29.307996821488736 21.09792719789519 20-21 22.665427511737025 26.53830152083483 29.470537316944768 21.325733650483382 22-23 22.283932157047037 26.37445734510715 29.493182512792032 21.84842798505378 24-25 22.3615652145908 26.589659772107876 29.51197381734913 21.536801195952194 26-27 22.17966055463447 26.671256578828455 29.687124639119528 21.46195822741755 28-29 21.862935869724893 27.278774229317026 29.50300260972186 21.35528729123622 30-31 22.178068929526567 26.61792861757764 29.371225271399247 21.832777181496542 32-33 22.00675400780957 26.77941749640314 29.368859133980557 21.84496936180673 34-35 22.494211179079137 26.66038763903522 29.280030391720622 21.565370790165016 36-37 22.45300095237031 26.531860368972843 29.424036144063592 21.591102534593258 38-39 22.055358413951797 26.924113372159898 29.472081878870853 21.548446335017456 40-41 22.169031599107967 26.998811015947112 29.162249329101453 21.66990805584347 42-43 22.136023870110627 26.80095685539296 28.971465909561523 22.09155336493489 44-45 22.413170972489052 26.79522460888077 28.82527192505654 21.96633249357364 46-47 22.61978077736816 27.32563374690348 28.414549904795834 21.64003557093253 48-49 22.627306408880376 27.119418298260822 28.434333442657646 21.818941850201153 50-51 22.334924122573803 27.36372198674043 28.52608699367124 21.775266897014525 52-53 22.67403762289948 27.71667081836149 27.79248580481864 21.81680575392039 54-55 22.483366382576815 27.390866841015942 27.926566925211 22.199199851196248 56-57 22.571044919147113 27.52767559204373 28.029592491983067 21.87168699682609 58-59 22.955213619486983 27.22684750967323 27.968171508156274 21.849767362683515 60-61 22.56772575415701 27.37857607164664 28.505383291257715 21.548314882938637 62-63 22.24027862582626 27.60086153692456 28.36436807370781 21.79449176354137 64-65 22.192627247255444 27.73832754834643 28.070145458297812 21.99889974610031 66-67 22.363449223676884 27.47972844629558 27.870568339635366 22.28625399039217 68-69 22.20734988008284 27.429119395951407 28.0847695020661 22.278761221899654 70-71 22.571965083698824 27.540952252004153 27.847892856039596 22.039189808257426 72-73 22.597668233468475 27.288839141498205 28.01442294578677 22.09906967924654 74-75 22.214908374614765 28.009874680160664 27.621203746121342 22.154013199103233 76-77 22.35690948275579 27.55577347389066 27.724327901952524 22.36298914140103 78-79 22.571209234245632 27.613020854215044 27.720943010923012 22.09482690061631 80-81 22.55323316246754 27.688835840672194 27.67237146780048 22.085559529059783 82-83 22.48815777971597 27.696960096959305 27.827328038867854 21.987554084456868 84-85 22.48093095708356 27.61184268902261 27.76429644186941 22.142929912024425 86-87 22.286549757569503 27.73671726038093 27.682953360145174 22.293779621904385 88-89 22.322337586027167 27.598429673466462 27.531454839309692 22.547777901196675 90-91 22.47378571597599 27.40477273504116 27.67569091860425 22.445750630378594 92-93 22.62541999463669 27.282135176489763 27.882944405592568 22.20950042328098 94-95 22.39420901011984 27.335426926775273 27.817921782069543 22.452442281035342 96-97 22.007641309341576 27.56507370846689 28.05568572962805 22.371599252563477 98-99 22.1490215512751 27.6872710470734 27.49360895374161 22.670098447909893 100-101 21.889002647489487 27.593381111945586 27.766264969161924 22.751351271403006 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 429.0 1 309.5 2 217.5 3 268.5 4 240.5 5 217.0 6 421.5 7 969.5 8 1651.5 9 2005.0 10 1845.5 11 1588.5 12 1589.5 13 1735.5 14 1923.0 15 2109.0 16 2215.5 17 2291.0 18 2443.0 19 2602.5 20 2851.0 21 3238.0 22 3768.5 23 4570.0 24 5546.5 25 6899.5 26 8830.5 27 11375.0 28 13556.0 29 15816.5 30 18645.0 31 20740.5 32 22980.5 33 26014.5 34 29357.0 35 32090.5 36 35610.5 37 38946.0 38 40803.5 39 42320.5 40 43950.5 41 47072.5 42 49999.0 43 52476.5 44 55248.0 45 57649.5 46 58811.0 47 62400.5 48 69843.0 49 68641.0 50 60872.0 51 55645.0 52 52421.0 53 50831.5 54 49248.5 55 45504.5 56 39731.0 57 34965.0 58 31004.5 59 27014.5 60 22587.0 61 18442.5 62 14539.0 63 10837.5 64 8119.5 65 6044.0 66 4356.5 67 3467.0 68 2721.0 69 1819.0 70 1258.5 71 885.5 72 661.5 73 489.5 74 388.5 75 289.5 76 177.0 77 100.5 78 57.5 79 36.0 80 17.5 81 6.5 82 6.5 83 4.5 84 2.0 85 3.5 86 1.5 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05540705122095977 2 0.0 3 0.0 4 0.0 5 0.019192003507141463 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003220575930992917 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.887124728962245E-4 24-25 9.858905911202806E-5 26-27 9.530275714162714E-4 28-29 0.002661904596024758 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.01537989322147638 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.6431509852004678E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.629041576320748E-4 84-85 0.0014459728669764117 86-87 0.0 88-89 0.0 90-91 0.010450440265874975 92-93 0.0012487947487523554 94-95 0.0 96-97 0.0 98-99 1.6431509852004678E-4 100-101 0.014098235453020011 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1521467.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.09736531812409 #Duplication Level Percentage of deduplicated Percentage of total 1 76.84257197326984 43.87508403942032 2 13.083916165904983 14.941142822327727 3 4.350154679807976 7.451471128300294 4 1.8703900640956082 4.271773791082259 5 1.0196790560220892 2.911049378446658 6 0.6005327498108399 2.0573302680868286 7 0.42949788164496044 1.7166238216149914 8 0.3028272332783636 1.383250973341721 9 0.21930738381742743 1.126968642970714 >10 1.1791057508510234 12.050877337388417 >50 0.06925816664363454 2.6855294192793657 >100 0.030230241768467392 2.9460160209312667 >500 0.0010344489892305003 0.44928253207098195 >1k 0.001149387765811667 1.041631952823638 >5k 3.4481632974350015E-4 1.0919678719148507 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6301 0.4141397743099259 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5317 0.34946535153243546 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5021 0.33001044386766193 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3664 0.24082020839098056 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1911 0.12560246130872377 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1786 0.11738670638272142 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 1.314520788160374E-4 7 0.0 0.0 0.0 0.0 1.314520788160374E-4 8 0.0 0.0 0.0 0.0 1.9717811822405612E-4 9 0.0 0.0 0.0 6.57260394080187E-5 1.9717811822405612E-4 10-11 0.0 0.0 0.0 6.57260394080187E-5 1.9717811822405612E-4 12-13 0.0 0.0 0.0 1.314520788160374E-4 1.9717811822405612E-4 14-15 0.0 0.0 0.0 1.314520788160374E-4 1.9717811822405612E-4 16-17 0.0 0.0 0.0 1.6431509852004678E-4 1.9717811822405612E-4 18-19 0.0 0.0 0.0 2.629041576320748E-4 1.9717811822405612E-4 20-21 0.0 0.0 0.0 2.629041576320748E-4 1.9717811822405612E-4 22-23 0.0 0.0 0.0 5.586713349681591E-4 1.9717811822405612E-4 24-25 0.0 0.0 0.0 7.558494531922152E-4 1.9717811822405612E-4 26-27 0.0 0.0 0.0 9.858905911202807E-4 1.9717811822405612E-4 28-29 0.0 0.0 0.0 0.0015116989063844303 1.9717811822405612E-4 30-31 0.0 0.0 0.0 0.002891945733952823 1.9717811822405612E-4 32-33 0.0 0.0 0.0 0.00506090503441744 1.9717811822405612E-4 34-35 0.0 0.0 0.0 0.006671192999913899 1.9717811822405612E-4 36-37 0.0 0.0 0.0 0.00765708359103418 1.9717811822405612E-4 38-39 0.0 0.0 0.0 0.008741563241266487 1.9717811822405612E-4 40-41 0.0 0.0 0.0 0.009891768930906816 1.9717811822405612E-4 42-43 0.0 0.0 0.0 0.012126454270779451 1.9717811822405612E-4 44-45 0.0 0.0 0.0 0.014163961492428032 1.9717811822405612E-4 46-47 0.0 0.0 0.0 0.017121633265788874 1.9717811822405612E-4 48-49 0.0 0.0 0.0 0.02070370241352589 1.9717811822405612E-4 50-51 0.0 0.0 0.0 0.024712990817415036 1.9717811822405612E-4 52-53 0.0 0.0 0.0 0.041505993886163814 2.3004113792806546E-4 54-55 0.0 0.0 0.0 0.06023791511744915 2.629041576320748E-4 56-57 0.0 0.0 0.0 0.08199323416150334 2.629041576320748E-4 58-59 0.0 0.0 0.0 0.11390322629409642 2.629041576320748E-4 60-61 0.0 0.0 0.0 0.1620146871407661 3.2863019704009355E-4 62-63 0.0 0.0 0.0 0.19198576111082266 3.2863019704009355E-4 64-65 0.0 0.0 0.0 0.22290986265229545 3.2863019704009355E-4 66-67 0.0 0.0 0.0 0.2923165602671632 3.2863019704009355E-4 68-69 0.0 0.0 0.0 0.3796664666404201 3.2863019704009355E-4 70-71 0.0 0.0 0.0 0.4493032053932159 3.2863019704009355E-4 72-73 0.0 0.0 0.0 0.5228506434907889 3.9435623644811223E-4 74-75 0.0 0.0 0.0 0.6651803818288533 3.9435623644811223E-4 76-77 0.0 0.0 0.0 0.7628821394088732 3.9435623644811223E-4 78-79 0.0 0.0 0.0 0.8452040037674167 3.9435623644811223E-4 80-81 0.0 0.0 0.0 0.9654497928643868 4.272192561521216E-4 82-83 0.0 0.0 0.0 1.15924302005893 4.6008227585613096E-4 84-85 0.0 0.0 0.0 1.3440975058939826 4.6008227585613096E-4 86-87 0.0 0.0 0.0 1.591556044265173 4.6008227585613096E-4 88-89 0.0 0.0 0.0 1.9338901205218386 4.6008227585613096E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 5495 0.0 22.402594 1 TACATGG 5545 0.0 22.18891 2 ACATGGG 5480 0.0 21.931974 3 GAGTACT 6075 0.0 21.30595 12-13 AGTACTT 6235 0.0 21.025839 12-13 GTATCAA 11875 0.0 20.572912 1 AGAGTAC 9305 0.0 20.469568 10-11 GTACTTT 6340 0.0 20.302357 14-15 ACGATCG 60 0.00476609 19.791079 82-83 GGTATCA 9525 0.0 18.11373 1 CATGGGG 3080 0.0 18.045666 4 TCAACGC 13660 0.0 17.770842 4 ATCAACG 13695 0.0 17.725424 3 CAACGCA 13700 0.0 17.718956 5 TACTTTT 7340 0.0 17.633432 14-15 TATCAAC 13935 0.0 17.488323 2 GTCTAGC 300 7.629751E-8 17.427603 1 TAAGGTG 655 0.0 17.406351 5 AACGCAG 13945 0.0 17.40479 6 ACTTTTT 7525 0.0 17.263323 16-17 >>END_MODULE