FastQCFastQC Report
Thu 26 May 2016
SRR937372_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937372_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1643655
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58400.35530570588110033No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43730.26605339928391303No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT38060.23155710900401846No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32790.199494419449337No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT26900.16365964877057534No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26010.15824488715697638No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT20320.12362691684082122No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC16630.10117695015073114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG62550.025.6764471
TACATGG61950.025.4618112
ACATGGG62750.024.1521723
GTATCAA104000.022.204881
GAGTACT50650.021.569912-13
GTATAGC7650.020.4973641
CATGGGG31450.020.2443184
AGTACTT55200.019.70589412-13
GGTATCA82050.019.5741831
AGAGTAC83450.019.52364310-11
GTACTTT54900.019.24912614-15
ATCAACG117500.019.2059153
TCAACGC118400.019.1017994
CAACGCA118000.019.0848545
TATCAAC120000.019.0043262
GAACCGT1759.9273486E-519.0031666
TAGTCGC1507.717488E-419.0020077
AACGCAG120150.018.8212816
ACCGTGC2803.4879122E-818.6621178
CATGGGA33000.018.4295644
Produced by FastQC (version 0.11.5)