FastQCFastQC Report
Thu 26 May 2016
SRR937372_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937372_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1643655
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66070.40196999978705994No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56280.3424076220374714No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT51840.31539465398760685No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40400.24579367324651463No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20560.12508707727594903No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT18170.11054631294280125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG60450.024.2991051
TACATGG60700.023.6321222
ACATGGG60300.022.6073173
GAGTACT62750.021.61504212-13
GTATACG1100.002865053721.6075441
AGTACTT64950.021.06575612-13
GTACTTT66150.020.49907514-15
CCATATA6750.020.4069752
GTATCAA128900.020.3570211
AGAGTAC97850.020.31881310-11
ACCGTGC3557.8216544E-1118.7354188
GTATAGG5000.018.0639061
CAACGCA146250.017.862875
TCAACGC146800.017.7959464
ATCAACG147450.017.749713
CACCGTT2704.868325E-717.5954347
TATCAAC148700.017.568562
GGTATCA103950.017.5146871
AACGCAG149500.017.4734846
TACTTTT78500.017.36481714-15
Produced by FastQC (version 0.11.5)